MC10g1199 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g1199
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Calcium-binding EF-hand family protein
Location	MC10: 15146075 .. 15147184 (-)
RNA-Seq Expression	MC10g1199
Synteny	MC10g1199

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCAAAGATCTGAGCGACGACCAGAAATCCTCCATGAAAGAGGCCTTCACTCTCTTCGATACCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATTCTCATGCGCTCCCTCGGCGGCAACCCCACTCAAGCTCAGCTCAAAGCCATCATCGCCGAGGAGAGCCTCACTTCCCCCTTCGATTTCAATCGCTTCCTCGACCTCATGTCCAAGCACATGAAGCCCGAGCCGTTCGATCGCCAGCTCCGCGACGCCTTTAAGGTTCTCGACAAGGACAACACCGGCTTCGTCTTGGTCTCCGAACTCCGCCACATTTTGACCAGTATAGGTGAGAAGCTCGAGCCGTCCGAGTTCGATGAGTGGATTCGAGAAGTCGATGTCGGATCGGACGGTAGAATTCGGTATGAGGATTTCATTGCCAGAATGGTGGCCAAATGAGGAGGGAAGTAATGGCAATTGGTTTCCTCTTTCGTGTGCTTTACTGGAATCCTTTCTGAAGTAATTTGTGGCGATGTTTGCTGTTCCTTTTATTTTCCCCCTTTTTCTTTTCTATTTCTGTTAATTGGTTTCATATTACGAGACTTGCTTGGATTTTTGGTGGAATGGATGCGGGTCATGTTCGCATGCTTTCCCAAGTAATGCTAATATTATTTGAAAAGATTTAGGATTCTGATGGGGTGGATATGTTTGGATGGATTCGAGGTGTATTGAAAACTGTGGTTTTCTGGGTAACGTAGAATCTGGTGAACTGTATGTGTTTCTCAGCTTGAATTCTCCCACAATTTATTGTCTTGATGGTTGCTATTTCTGCAGCCTCGTTTAGTAAGGGAGACAGAGATGGTGTCTTTTTGTTCACAATGCCTTATGAGATAAGAAACCTGATGTGCATGTTTTAACTTTAAATCAAATCCACCTGATATATGTTAGGTGTCCAAGAACTAATCATATAGTATGTGTTTAAGATGATTACAAGGTTCTGTTGCTACAATGTATATATTTTGAATTTGAATGTAGTGAACCGAGGCTAAAATGAGCATCAAAAGCTTGGCTCTAGACTCCATTTTTTTACTCAATCTACTTTCTTTCCAGAAAAAGAATTCCTCA

mRNA sequence

ATGGGCAAAGATCTGAGCGACGACCAGAAATCCTCCATGAAAGAGGCCTTCACTCTCTTCGATACCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATTCTCATGCGCTCCCTCGGCGGCAACCCCACTCAAGCTCAGCTCAAAGCCATCATCGCCGAGGAGAGCCTCACTTCCCCCTTCGATTTCAATCGCTTCCTCGACCTCATGTCCAAGCACATGAAGCCCGAGCCGTTCGATCGCCAGCTCCGCGACGCCTTTAAGGTTCTCGACAAGGACAACACCGGCTTCGTCTTGGTCTCCGAACTCCGCCACATTTTGACCAGTATAGGTGAGAAGCTCGAGCCGTCCGAGTTCGATGAGTGGATTCGAGAAGTCGATGTCGGATCGGACGGTAGAATTCGGTATGAGGATTTCATTGCCAGAATGGTGGCCAAATGAGGAGGGAAGTAATGGCAATTGGTTTCCTCTTTCGTGTGCTTTACTGGAATCCTTTCTGAAGTAATTTGTGGCGATGTTTGCTGTTCCTTTTATTTTCCCCCTTTTTCTTTTCTATTTCTGTTAATTGGTTTCATATTACGAGACTTGCTTGGATTTTTGGTGGAATGGATGCGGGTCATGTTCGCATGCTTTCCCAAGTAATGCTAATATTATTTGAAAAGATTTAGGATTCTGATGGGGTGGATATGTTTGGATGGATTCGAGGTGTATTGAAAACTGTGGTTTTCTGGGTAACGTAGAATCTGGTGAACTGTATGTGTTTCTCAGCTTGAATTCTCCCACAATTTATTGTCTTGATGGTTGCTATTTCTGCAGCCTCGTTTAGTAAGGGAGACAGAGATGGTGTCTTTTTGTTCACAATGCCTTATGAGATAAGAAACCTGATGTGCATGTTTTAACTTTAAATCAAATCCACCTGATATATGTTAGGTGTCCAAGAACTAATCATATAGTATGTGTTTAAGATGATTACAAGGTTCTGTTGCTACAATGTATATATTTTGAATTTGAATGTAGTGAACCGAGGCTAAAATGAGCATCAAAAGCTTGGCTCTAGACTCCATTTTTTTACTCAATCTACTTTCTTTCCAGAAAAAGAATTCCTCA

Coding sequence (CDS)

ATGGGCAAAGATCTGAGCGACGACCAGAAATCCTCCATGAAAGAGGCCTTCACTCTCTTCGATACCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATTCTCATGCGCTCCCTCGGCGGCAACCCCACTCAAGCTCAGCTCAAAGCCATCATCGCCGAGGAGAGCCTCACTTCCCCCTTCGATTTCAATCGCTTCCTCGACCTCATGTCCAAGCACATGAAGCCCGAGCCGTTCGATCGCCAGCTCCGCGACGCCTTTAAGGTTCTCGACAAGGACAACACCGGCTTCGTCTTGGTCTCCGAACTCCGCCACATTTTGACCAGTATAGGTGAGAAGCTCGAGCCGTCCGAGTTCGATGAGTGGATTCGAGAAGTCGATGTCGGATCGGACGGTAGAATTCGGTATGAGGATTTCATTGCCAGAATGGTGGCCAAATGA

Protein sequence

MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSPFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFDEWIREVDVGSDGRIRYEDFIARMVAK

Homology

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BLAST of MC10g1199 vs. ExPASy Swiss-Prot
Match: Q94AZ4 (Probable calcium-binding protein CML13 OS=Arabidopsis thaliana OX=3702 GN=CML13 PE=2 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 9.5e-69
Identity = 129/148 (87.16%), Postives = 141/148 (95.27%), Query Frame = 0


Query: 1   MGKD-LSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLT 60
           MGKD LSDDQ SSMKEAF LFDTDGDG+IAPSELGILMRSLGGNPTQAQLK+IIA E+L+
Sbjct: 1   MGKDGLSDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIIASENLS 60

Query: 61  SPFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSE 120
           SPFDFNRFLDLM+KH+K EPFDRQLRDAFKVLDK+ TGFV V++LRHILTSIGEKLEP+E
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLEPNE 120

Query: 121 FDEWIREVDVGSDGRIRYEDFIARMVAK 148
           FDEWI+EVDVGSDG+IRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

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BLAST of MC10g1199 vs. ExPASy Swiss-Prot
Match: Q8VZ50 (Probable calcium-binding protein CML14 OS=Arabidopsis thaliana OX=3702 GN=CML14 PE=2 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 3.4e-66
Identity = 124/148 (83.78%), Postives = 139/148 (93.92%), Query Frame = 0


Query: 1   MGKD-LSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLT 60
           M KD LS+DQ SSMKEAF LFDTDGDG+IAPSELGILMRSLGGNPT++QLK+II  E+L+
Sbjct: 1   MSKDGLSNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTESQLKSIITTENLS 60

Query: 61  SPFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSE 120
           SPFDFNRFLDLM+KH+K EPFDRQLRDAFKVLDK+ TGFV V++LRHILTSIGEKL+PSE
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLQPSE 120

Query: 121 FDEWIREVDVGSDGRIRYEDFIARMVAK 148
           FDEWI+EVDVGSDG+IRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of MC10g1199 vs. ExPASy Swiss-Prot
Match: Q84VG0 (Probable calcium-binding protein CML7 OS=Oryza sativa subsp. japonica OX=39947 GN=CML7 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 3.6e-60
Identity = 111/146 (76.03%), Postives = 130/146 (89.04%), Query Frame = 0


Query: 2   GKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTSP 61
           GK+LS++Q +SM+EAF+LFDTDGDGRIAPSELG+LMRSLGGNPTQAQL+ I A+E LT+P
Sbjct: 3   GKELSEEQVASMREAFSLFDTDGDGRIAPSELGVLMRSLGGNPTQAQLRDIAAQEKLTAP 62

Query: 62  FDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEFD 121
           FDF RFLDLM  H++PEPFDR LRDAF+VLDKD +G V V++LRH+LTSIGEKLEP EFD
Sbjct: 63  FDFPRFLDLMRAHLRPEPFDRPLRDAFRVLDKDASGTVSVADLRHVLTSIGEKLEPHEFD 122

Query: 122 EWIREVDVGSDGRIRYEDFIARMVAK 148
           EWIREVDV  DG IRY+DFI R+VAK
Sbjct: 123 EWIREVDVAPDGTIRYDDFIRRIVAK 148

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BLAST of MC10g1199 vs. ExPASy Swiss-Prot
Match: Q03509 (Calmodulin-6 OS=Arabidopsis thaliana OX=3702 GN=CAM6 PE=1 SV=2)

HSP 1 Score: 152.5 bits (384), Expect = 3.6e-36
Identity = 76/149 (51.01%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESL-- 60
           M   L+DDQ S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E     
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TSPFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPS 120
               DF  FL+LM++ MK    + +L++AF+V DKD  GF+  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDE 120

Query: 121 EFDEWIREVDVGSDGRIRYEDFIARMVAK 148
           E DE IRE DV  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKVMMAK 149

[Close]

BLAST of MC10g1199 vs. ExPASy Swiss-Prot
Match: P62200 (Calmodulin-1/11/16 OS=Daucus carota OX=4039 GN=CAM-1 PE=2 SV=2)

HSP 1 Score: 152.1 bits (383), Expect = 4.8e-36
Identity = 76/149 (51.01%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESL-- 60
           M   L+DDQ S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E     
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TSPFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPS 120
               DF  FL+LM++ MK    + +L++AF+V DKD  GF+  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVDVGSDGRIRYEDFIARMVAK 148
           E DE IRE DV  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKVMMAK 149

[Close]

BLAST of MC10g1199 vs. NCBI nr
Match: XP_022152721.1 (probable calcium-binding protein CML13 [Momordica charantia])

HSP 1 Score: 293 bits (751), Expect = 4.03e-100
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS 60
           MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS
Sbjct: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS 60

Query: 61  PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVDVGSDGRIRYEDFIARMVAK 147
           DEWIREVDVGSDGRIRYEDFIARMVAK
Sbjct: 121 DEWIREVDVGSDGRIRYEDFIARMVAK 147

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BLAST of MC10g1199 vs. NCBI nr
Match: XP_038876882.1 (probable calcium-binding protein CML13 [Benincasa hispida])

HSP 1 Score: 285 bits (729), Expect = 9.14e-97
Identity = 140/147 (95.24%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS 60
           MGKDLSDDQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LT+
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL++MSKHMKPEPFDRQLRDAFKVLDKDNTG+VLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVDVGSDGRIRYEDFIARMVAK 147
           DEWIREV+VGSDGRIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 147

[Close]

BLAST of MC10g1199 vs. NCBI nr
Match: XP_008448907.1 (PREDICTED: probable calcium-binding protein CML13 [Cucumis melo] >QNH87118.1 CML13 [Cucumis melo subsp. melo] >TYK19478.1 putative calcium-binding protein CML13 [Cucumis melo var. makuwa])

HSP 1 Score: 284 bits (727), Expect = 1.85e-96
Identity = 141/147 (95.92%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS 60
           MGKDLSDDQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTS
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLD+MSKHMKPEPFDRQLRDAFKVLDKDNTGFV VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGFVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVDVGSDGRIRYEDFIARMVAK 147
           DEWIREV+VGSDG IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGSIRYEDFIARMVAK 147

[Close]

BLAST of MC10g1199 vs. NCBI nr
Match: XP_004147942.1 (probable calcium-binding protein CML13 [Cucumis sativus] >KGN55913.1 hypothetical protein Csa_011609 [Cucumis sativus])

HSP 1 Score: 283 bits (724), Expect = 5.29e-96
Identity = 140/147 (95.24%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS 60
           MGKDLSDDQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTS
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLD+MSKHMKPEPFDRQLRDAFKVLDKDNTG+V VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVDVGSDGRIRYEDFIARMVAK 147
           DEWIREV+VGSDG IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGSIRYEDFIARMVAK 147

[Close]

BLAST of MC10g1199 vs. NCBI nr
Match: XP_023005593.1 (probable calcium-binding protein CML13 [Cucurbita maxima])

HSP 1 Score: 278 bits (711), Expect = 5.08e-94
Identity = 137/147 (93.20%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS 60
           MGKDLS+DQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTS
Sbjct: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL++MSKHMKPE FDRQLRDAFKVLDKDNTG+VLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVDVGSDGRIRYEDFIARMVAK 147
           DEWIREV+VG DG+IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of MC10g1199 vs. ExPASy TrEMBL
Match: A0A6J1DIL4 (probable calcium-binding protein CML13 OS=Momordica charantia OX=3673 GN=LOC111020377 PE=4 SV=1)

HSP 1 Score: 293 bits (751), Expect = 1.95e-100
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS 60
           MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS
Sbjct: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS 60

Query: 61  PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVDVGSDGRIRYEDFIARMVAK 147
           DEWIREVDVGSDGRIRYEDFIARMVAK
Sbjct: 121 DEWIREVDVGSDGRIRYEDFIARMVAK 147

[Close]

BLAST of MC10g1199 vs. ExPASy TrEMBL
Match: A0A5D3D7C6 (Putative calcium-binding protein CML13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G001330 PE=4 SV=1)

HSP 1 Score: 284 bits (727), Expect = 8.93e-97
Identity = 141/147 (95.92%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS 60
           MGKDLSDDQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTS
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLD+MSKHMKPEPFDRQLRDAFKVLDKDNTGFV VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGFVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVDVGSDGRIRYEDFIARMVAK 147
           DEWIREV+VGSDG IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGSIRYEDFIARMVAK 147

[Close]

BLAST of MC10g1199 vs. ExPASy TrEMBL
Match: A0A7G7Y0U3 (CML13 OS=Cucumis melo subsp. melo OX=412675 PE=2 SV=1)

HSP 1 Score: 284 bits (727), Expect = 8.93e-97
Identity = 141/147 (95.92%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS 60
           MGKDLSDDQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTS
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLD+MSKHMKPEPFDRQLRDAFKVLDKDNTGFV VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGFVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVDVGSDGRIRYEDFIARMVAK 147
           DEWIREV+VGSDG IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGSIRYEDFIARMVAK 147

[Close]

BLAST of MC10g1199 vs. ExPASy TrEMBL
Match: A0A1S3BLQ3 (probable calcium-binding protein CML13 OS=Cucumis melo OX=3656 GN=LOC103490931 PE=4 SV=1)

HSP 1 Score: 284 bits (727), Expect = 8.93e-97
Identity = 141/147 (95.92%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS 60
           MGKDLSDDQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTS
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLD+MSKHMKPEPFDRQLRDAFKVLDKDNTGFV VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGFVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVDVGSDGRIRYEDFIARMVAK 147
           DEWIREV+VGSDG IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGSIRYEDFIARMVAK 147

[Close]

BLAST of MC10g1199 vs. ExPASy TrEMBL
Match: A0A0A0L2F2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G036390 PE=4 SV=1)

HSP 1 Score: 283 bits (724), Expect = 2.56e-96
Identity = 140/147 (95.24%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS 60
           MGKDLSDDQKSSM+EAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTS
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLD+MSKHMKPEPFDRQLRDAFKVLDKDNTG+V VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVDVGSDGRIRYEDFIARMVAK 147
           DEWIREV+VGSDG IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGSIRYEDFIARMVAK 147

[Close]

BLAST of MC10g1199 vs. TAIR 10
Match: AT1G12310.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 260.8 bits (665), Expect = 6.8e-70
Identity = 129/148 (87.16%), Postives = 141/148 (95.27%), Query Frame = 0


Query: 1   MGKD-LSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLT 60
           MGKD LSDDQ SSMKEAF LFDTDGDG+IAPSELGILMRSLGGNPTQAQLK+IIA E+L+
Sbjct: 1   MGKDGLSDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIIASENLS 60

Query: 61  SPFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSE 120
           SPFDFNRFLDLM+KH+K EPFDRQLRDAFKVLDK+ TGFV V++LRHILTSIGEKLEP+E
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLEPNE 120

Query: 121 FDEWIREVDVGSDGRIRYEDFIARMVAK 148
           FDEWI+EVDVGSDG+IRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of MC10g1199 vs. TAIR 10
Match: AT1G62820.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 252.3 bits (643), Expect = 2.4e-67
Identity = 124/148 (83.78%), Postives = 139/148 (93.92%), Query Frame = 0


Query: 1   MGKD-LSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLT 60
           M KD LS+DQ SSMKEAF LFDTDGDG+IAPSELGILMRSLGGNPT++QLK+II  E+L+
Sbjct: 1   MSKDGLSNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTESQLKSIITTENLS 60

Query: 61  SPFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSE 120
           SPFDFNRFLDLM+KH+K EPFDRQLRDAFKVLDK+ TGFV V++LRHILTSIGEKL+PSE
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLQPSE 120

Query: 121 FDEWIREVDVGSDGRIRYEDFIARMVAK 148
           FDEWI+EVDVGSDG+IRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of MC10g1199 vs. TAIR 10
Match: AT5G21274.1 (calmodulin 6 )

HSP 1 Score: 152.5 bits (384), Expect = 2.6e-37
Identity = 76/149 (51.01%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESL-- 60
           M   L+DDQ S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E     
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TSPFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPS 120
               DF  FL+LM++ MK    + +L++AF+V DKD  GF+  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDE 120

Query: 121 EFDEWIREVDVGSDGRIRYEDFIARMVAK 148
           E DE IRE DV  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKVMMAK 149

[Close]

BLAST of MC10g1199 vs. TAIR 10
Match: AT3G43810.1 (calmodulin 7 )

HSP 1 Score: 152.1 bits (383), Expect = 3.4e-37
Identity = 76/149 (51.01%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESL-- 60
           M   L+DDQ S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E     
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TSPFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPS 120
               DF  FL+LM++ MK    + +L++AF+V DKD  GF+  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVDVGSDGRIRYEDFIARMVAK 148
           E DE IRE DV  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKVMMAK 149

[Close]

BLAST of MC10g1199 vs. TAIR 10
Match: AT2G41110.1 (calmodulin 2 )

HSP 1 Score: 151.0 bits (380), Expect = 7.5e-37
Identity = 75/149 (50.34%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESL-- 60
           M   L+DDQ S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E     
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TSPFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPS 120
               DF  FL+LM++ MK    + +L++AF+V DKD  GF+  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVDVGSDGRIRYEDFIARMVAK 148
           E DE I+E DV  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIKEADVDGDGQINYEEFVKVMMAK 149

The following BLAST results are available for this feature:

BLAST of MC10g1199 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AZ4	9.5e-69	87.16	Probable calcium-binding protein CML13 OS=Arabidopsis thaliana OX=3702 GN=CML13 ...	[more]
Q8VZ50	3.4e-66	83.78	Probable calcium-binding protein CML14 OS=Arabidopsis thaliana OX=3702 GN=CML14 ...	[more]
Q84VG0	3.6e-60	76.03	Probable calcium-binding protein CML7 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q03509	3.6e-36	51.01	Calmodulin-6 OS=Arabidopsis thaliana OX=3702 GN=CAM6 PE=1 SV=2	[more]
P62200	4.8e-36	51.01	Calmodulin-1/11/16 OS=Daucus carota OX=4039 GN=CAM-1 PE=2 SV=2	[more]

BLAST of MC10g1199 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152721.1	4.03e-100	100.00	probable calcium-binding protein CML13 [Momordica charantia]	[more]
XP_038876882.1	9.14e-97	95.24	probable calcium-binding protein CML13 [Benincasa hispida]	[more]
XP_008448907.1	1.85e-96	95.92	PREDICTED: probable calcium-binding protein CML13 [Cucumis melo] >QNH87118.1 CML...	[more]
XP_004147942.1	5.29e-96	95.24	probable calcium-binding protein CML13 [Cucumis sativus] >KGN55913.1 hypothetica...	[more]
XP_023005593.1	5.08e-94	93.20	probable calcium-binding protein CML13 [Cucurbita maxima]	[more]

BLAST of MC10g1199 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DIL4	1.95e-100	100.00	probable calcium-binding protein CML13 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A5D3D7C6	8.93e-97	95.92	Putative calcium-binding protein CML13 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A7G7Y0U3	8.93e-97	95.92	CML13 OS=Cucumis melo subsp. melo OX=412675 PE=2 SV=1	[more]
A0A1S3BLQ3	8.93e-97	95.92	probable calcium-binding protein CML13 OS=Cucumis melo OX=3656 GN=LOC103490931 P...	[more]
A0A0A0L2F2	2.56e-96	95.24	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G036390 PE=4 SV=1	[more]

BLAST of MC10g1199 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12310.1	6.8e-70	87.16	Calcium-binding EF-hand family protein	[more]
AT1G62820.1	2.4e-67	83.78	Calcium-binding EF-hand family protein	[more]
AT5G21274.1	2.6e-37	51.01	calmodulin 6	[more]
AT3G43810.1	3.4e-37	51.01	calmodulin 7	[more]
AT2G41110.1	7.5e-37	50.34	calmodulin 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 12..40 e-value: 8.4E-5 score: 31.9 coord: 83..111 e-value: 5.3E-4 score: 29.3 coord: 119..147 e-value: 0.12 score: 21.5
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 82..144 e-value: 2.1E-14 score: 53.7
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 13..41 e-value: 3.6E-6 score: 26.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 115..147 score: 9.26907
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 8..43 score: 13.062845
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 79..114 score: 12.253878
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 14..55 e-value: 1.85777E-8 score: 46.3869
None	No IPR available	GENE3D	1.10.238.10		coord: 3..80 e-value: 7.3E-20 score: 72.9
None	No IPR available	GENE3D	1.10.238.10		coord: 81..145 e-value: 3.0E-19 score: 70.9
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 1..147
None	No IPR available	PANTHER	PTHR23050:SF412	SUBFAMILY NOT NAMED	coord: 1..147
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 21..33
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 5..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g1199.1	MC10g1199.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding