MC10g1194 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g1194
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionIRK-interacting protein
LocationMC10: 15077848 .. 15080087 (-)
RNA-Seq ExpressionMC10g1194
SyntenyMC10g1194
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTGGAATTCCCACGTTGTACTTAAAAAAAGAACTTCTCTTATTTATATTATGATAAATAAAATATAAGCATAAATAGGAATAAAAATAAAAAATAATTTAAGAAAACAATTTCGAAAGAGTAAGAAAAATATATTTCGGTCATTGTCATTATTTTGCTACTTTTAATATGTGCATTCAAATAAGTCGAAACTTATCTTAACTCACCAGCGTGACACCGTTACACAATCATATAAATACATTAATACAAATATTTGAAGTCATATTACGGACAAAGAATAAAAGTAGACATTTTGAAAATTTTCAAGTAAAAATAATCCCTATTAAAAATAATCCCTATTTTTTTAGGCAGCCCCATATTTTTACATACCTTTTTTATTTTCTTGGACAATGTTTCACTATTTTATTGAATATATAACAATTTATGTAAGAAAAAATATTTTAAAAAATGAATGAAGATGAAAAAAAACAGAGAAAAAAACAAAAAATCACCAACAAACTCAAACTCTCTCTCTCTCATATATATATATATATATCTAACACCAATGCTCAGACTGGAAAAAAAAAAGCAAAAATTAAGAAACTAAAATTAAAAAAAAATGAAAAATATCACTCCTTCTTCTCCCTCTCCTCTCCACCATTACCACTCTCCCCATTTCTTCCCTGTAATTCGCTTTCTCTTTCAGTTTAATTCAGCTTCAATTTCTGTTCAAAATCATCAAAAAGATGAACCCGATTGAATAATTTGTTTAATTTTTTTTGTTACAGATCATTCAGGACAGTGAGAGAACAGAGGAAGAAGATGAGTTCACTAACCGTACGGACGCCGGAGCGAAGCACCACCCGACGCCGCTCCACCACCATTCGCACGGCGGCGACGGAGTTCTTCCCAAACGCTGCAGCAAGAAACGCCCGGAGTCGGCCGGCTATGTCCGCGGCGGCGGAGGAGCGGTCTCCTGCAGCAAGTGCCGACCACACGCTCGAGAAAAATTCTCCGTTGTTCCGTTGGACAACAATGGCGTCGCCAAGCAATCTTTCTCCATGGCGAGCCCTAACGGAATTTTCAAGTCGATTGTGTCGTCGCTGACGCGGAGAAGCCCTAAATCGATGAACGAAGCGGTGGATTGGGCGGCGAGAGAAGAGCATTGGAAGATAGCATTGGGGGAAATTTCACACAAATTGATTCAGGCGACGAGGAAGAGAGATGAAGCGGTTCTCGAAGCTTCGAGGCTGAAATATTCCATGGCGGAATTGGAGAACAAACTCAATAAACTCGAAATTTACTGCCATACCTTGAAATCTGGACTCGACGAATGCGCTGCAAATCCGCGGAAATCGAAACCAGAAGTTCTAATCAGCGATTCCGTTTCGGACAAAATAATCGAGAATTTCTTGGCTTCGGTTTCCGAATCTCGATCGTCAGTTCGGCAATTAAGCCGGTCACTGACGATGCAACTCCGTCACATGGGAGGCAAAATCCACGAGAGGCTCCAATTTCTCCTCCAACCTAACGAAATCAAAATCCCCCAATCCAAAAACCCTAGAATCTCACTCGCCTTCCACCTCGAGGCGCTGCTTAACAGAGCATTCTTCGAGGACTTCGAATCGATAGGGTTTCAGAAGAACTCACCGAATCAGATCCTGAACCCTTCGGATCGGACCGAATCGAACATCGCGGCGTTCGGTCTCCTCCACCGCCTGACCTGGGAGGAGGTTCTGAGCAAAGGAACGAGGCATTTCAGCGAGGAGTTCAGCAGATTCTGCGATCGGAAAATGAGCGACATCGTAGCAATGCTGGAGTGGAACAGAGCCTGGCCGGAGCCGCTGCTGCAGGCGTTCTTCGCGGCGGCGAAGAGCGTGTGGCTGGTTCATCTTCTCGCCACCGCCGTCCACCCGAGCTTGCCGATCTTCAGAGTGGATTCCGGAGCGAGGTTCGACGGCGTTTACATGGAGGACATCGCCGGCGACGAAACAGCTCCGGCGACGGTGAGAATCATGGTATCGCCGGGATTTTATGTGTTTGATAATTTGATAAAGTGTAAGGTTCTGTGTAGGTACCACCACCAATCATAATAATTAATTCATGTTTTAATATGAAATTATTTTTTTACTTAAATTTCTTTTTCTTTTTCCTCTCAAATTTAATTCCCATTATTTTATGTTTAATTTCAATTCAGTGTCTATAATTTTAAAATTGTATATTTAAGTTTCCATATTTTTATTTAACGTACCAAATCTTCT

mRNA sequence

GTTGGAATTCCCACGTTGTACTTAAAAAAAGAACTTCTCTTATTTATATTATGATAAATAAAATATAAGCATAAATAGGAATAAAAATAAAAAATAATTTAAGAAAACAATTTCGAAAGAGTAAGAAAAATATATTTCGGTCATTGTCATTATTTTGCTACTTTTAATATGTGCATTCAAATAAGTCGAAACTTATCTTAACTCACCAGCGTGACACCGTTACACAATCATATAAATACATTAATACAAATATTTGAAGTCATATTACGGACAAAGAATAAAAGTAGACATTTTGAAAATTTTCAAGTAAAAATAATCCCTATTAAAAATAATCCCTATTTTTTTAGGCAGCCCCATATTTTTACATACCTTTTTTATTTTCTTGGACAATGTTTCACTATTTTATTGAATATATAACAATTTATGTAAGAAAAAATATTTTAAAAAATGAATGAAGATGAAAAAAAACAGAGAAAAAAACAAAAAATCACCAACAAACTCAAACTCTCTCTCTCTCATATATATATATATATATCTAACACCAATGCTCAGACTGGAAAAAAAAAAGCAAAAATTAAGAAACTAAAATTAAAAAAAAATGAAAAATATCACTCCTTCTTCTCCCTCTCCTCTCCACCATTACCACTCTCCCCATTTCTTCCCTATCATTCAGGACAGTGAGAGAACAGAGGAAGAAGATGAGTTCACTAACCGTACGGACGCCGGAGCGAAGCACCACCCGACGCCGCTCCACCACCATTCGCACGGCGGCGACGGAGTTCTTCCCAAACGCTGCAGCAAGAAACGCCCGGAGTCGGCCGGCTATGTCCGCGGCGGCGGAGGAGCGGTCTCCTGCAGCAAGTGCCGACCACACGCTCGAGAAAAATTCTCCGTTGTTCCGTTGGACAACAATGGCGTCGCCAAGCAATCTTTCTCCATGGCGAGCCCTAACGGAATTTTCAAGTCGATTGTGTCGTCGCTGACGCGGAGAAGCCCTAAATCGATGAACGAAGCGGTGGATTGGGCGGCGAGAGAAGAGCATTGGAAGATAGCATTGGGGGAAATTTCACACAAATTGATTCAGGCGACGAGGAAGAGAGATGAAGCGGTTCTCGAAGCTTCGAGGCTGAAATATTCCATGGCGGAATTGGAGAACAAACTCAATAAACTCGAAATTTACTGCCATACCTTGAAATCTGGACTCGACGAATGCGCTGCAAATCCGCGGAAATCGAAACCAGAAGTTCTAATCAGCGATTCCGTTTCGGACAAAATAATCGAGAATTTCTTGGCTTCGGTTTCCGAATCTCGATCGTCAGTTCGGCAATTAAGCCGGTCACTGACGATGCAACTCCGTCACATGGGAGGCAAAATCCACGAGAGGCTCCAATTTCTCCTCCAACCTAACGAAATCAAAATCCCCCAATCCAAAAACCCTAGAATCTCACTCGCCTTCCACCTCGAGGCGCTGCTTAACAGAGCATTCTTCGAGGACTTCGAATCGATAGGGTTTCAGAAGAACTCACCGAATCAGATCCTGAACCCTTCGGATCGGACCGAATCGAACATCGCGGCGTTCGGTCTCCTCCACCGCCTGACCTGGGAGGAGGTTCTGAGCAAAGGAACGAGGCATTTCAGCGAGGAGTTCAGCAGATTCTGCGATCGGAAAATGAGCGACATCGTAGCAATGCTGGAGTGGAACAGAGCCTGGCCGGAGCCGCTGCTGCAGGCGTTCTTCGCGGCGGCGAAGAGCGTGTGGCTGGTTCATCTTCTCGCCACCGCCGTCCACCCGAGCTTGCCGATCTTCAGAGTGGATTCCGGAGCGAGGTTCGACGGCGTTTACATGGAGGACATCGCCGGCGACGAAACAGCTCCGGCGACGGTGAGAATCATGGTATCGCCGGGATTTTATGTGTTTGATAATTTGATAAAGTGTAAGGTTCTGTGTAGGTACCACCACCAATCATAATAATTAATTCATGTTTTAATATGAAATTATTTTTTTACTTAAATTTCTTTTTCTTTTTCCTCTCAAATTTAATTCCCATTATTTTATGTTTAATTTCAATTCAGTGTCTATAATTTTAAAATTGTATATTTAAGTTTCCATATTTTTATTTAACGTACCAAATCTTCT

Coding sequence (CDS)

ATGAAAAATATCACTCCTTCTTCTCCCTCTCCTCTCCACCATTACCACTCTCCCCATTTCTTCCCTATCATTCAGGACAGTGAGAGAACAGAGGAAGAAGATGAGTTCACTAACCGTACGGACGCCGGAGCGAAGCACCACCCGACGCCGCTCCACCACCATTCGCACGGCGGCGACGGAGTTCTTCCCAAACGCTGCAGCAAGAAACGCCCGGAGTCGGCCGGCTATGTCCGCGGCGGCGGAGGAGCGGTCTCCTGCAGCAAGTGCCGACCACACGCTCGAGAAAAATTCTCCGTTGTTCCGTTGGACAACAATGGCGTCGCCAAGCAATCTTTCTCCATGGCGAGCCCTAACGGAATTTTCAAGTCGATTGTGTCGTCGCTGACGCGGAGAAGCCCTAAATCGATGAACGAAGCGGTGGATTGGGCGGCGAGAGAAGAGCATTGGAAGATAGCATTGGGGGAAATTTCACACAAATTGATTCAGGCGACGAGGAAGAGAGATGAAGCGGTTCTCGAAGCTTCGAGGCTGAAATATTCCATGGCGGAATTGGAGAACAAACTCAATAAACTCGAAATTTACTGCCATACCTTGAAATCTGGACTCGACGAATGCGCTGCAAATCCGCGGAAATCGAAACCAGAAGTTCTAATCAGCGATTCCGTTTCGGACAAAATAATCGAGAATTTCTTGGCTTCGGTTTCCGAATCTCGATCGTCAGTTCGGCAATTAAGCCGGTCACTGACGATGCAACTCCGTCACATGGGAGGCAAAATCCACGAGAGGCTCCAATTTCTCCTCCAACCTAACGAAATCAAAATCCCCCAATCCAAAAACCCTAGAATCTCACTCGCCTTCCACCTCGAGGCGCTGCTTAACAGAGCATTCTTCGAGGACTTCGAATCGATAGGGTTTCAGAAGAACTCACCGAATCAGATCCTGAACCCTTCGGATCGGACCGAATCGAACATCGCGGCGTTCGGTCTCCTCCACCGCCTGACCTGGGAGGAGGTTCTGAGCAAAGGAACGAGGCATTTCAGCGAGGAGTTCAGCAGATTCTGCGATCGGAAAATGAGCGACATCGTAGCAATGCTGGAGTGGAACAGAGCCTGGCCGGAGCCGCTGCTGCAGGCGTTCTTCGCGGCGGCGAAGAGCGTGTGGCTGGTTCATCTTCTCGCCACCGCCGTCCACCCGAGCTTGCCGATCTTCAGAGTGGATTCCGGAGCGAGGTTCGACGGCGTTTACATGGAGGACATCGCCGGCGACGAAACAGCTCCGGCGACGGTGAGAATCATGGTATCGCCGGGATTTTATGTGTTTGATAATTTGATAAAGTGTAAGGTTCTGTGTAGGTACCACCACCAATCATAA

Protein sequence

MKNITPSSPSPLHHYHSPHFFPIIQDSERTEEEDEFTNRTDAGAKHHPTPLHHHSHGGDGVLPKRCSKKRPESAGYVRGGGGAVSCSKCRPHAREKFSVVPLDNNGVAKQSFSMASPNGIFKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVLEASRLKYSMAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLISDSVSDKIIENFLASVSESRSSVRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEALLNRAFFEDFESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEEFSRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDGVYMEDIAGDETAPATVRIMVSPGFYVFDNLIKCKVLCRYHHQS
Homology
BLAST of MC10g1194 vs. ExPASy Swiss-Prot
Match: Q9LXU9 (IRK-interacting protein OS=Arabidopsis thaliana OX=3702 GN=IRKI PE=1 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.1e-62
Identity = 135/351 (38.46%), Postives = 203/351 (57.83%), Query Frame = 0

Query: 118 NGIFKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVLEASRL 177
           N IF S   S+T +S +      D  +  E       ++  +L++A R RD A+ + S +
Sbjct: 222 NSIFNS--PSITEKSEEVSEVLKDSGSGVE-------KLKRELMEANRSRDAALTQVSEM 281

Query: 178 KYSMAELENKLNKLEIYCHTLKSGLDEC----------AANPRKSKPEVLISDSVSDKII 237
           K S+ EL  KL  LE YC  LK  L E             +  K   E+ +S+ V   ++
Sbjct: 282 KSSLGELSEKLQYLESYCDNLKKALREATEVVSQENSGGRSSGKKNSEMPVSEEV---MV 341

Query: 238 ENFLASVSESRSSVRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFH 297
           E FL  VSE+R S++Q  ++L  ++      +   +  LLQP+ +    SK  +I + +H
Sbjct: 342 EGFLQIVSEARLSIKQFLKTLVSEIDEEDSTLIGNINTLLQPHNLSF-TSKYSKI-IQYH 401

Query: 298 LEALLNRAFFEDFESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFS 357
           LEA+++++ ++DFE+  FQKN   ++L+P    ++N ++F  L  L+W EVL KGT+++S
Sbjct: 402 LEAIISQSVYQDFENCVFQKNGKPKLLDPEQDRQANFSSFASLRNLSWNEVLKKGTKYYS 461

Query: 358 EEFSRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDS 417
           +EFSRFCD KMS I+  L W R W E +LQAFF AAK VWL+HLLA + +P+L I RV+ 
Sbjct: 462 DEFSRFCDEKMSLIITTLNWTRPWSEQMLQAFFVAAKCVWLLHLLAFSFNPALGILRVEE 521

Query: 418 GARFDGVYMEDIAGD------ETAPATVRIMVSPGFYVFDNLIKCKVLCRY 453
              F+  +MED+  D         PA V++MV PGFYV D +++CKVLCRY
Sbjct: 522 NREFESSFMEDMGADRQRSALSRGPARVKVMVMPGFYVLDRVLRCKVLCRY 558

BLAST of MC10g1194 vs. ExPASy Swiss-Prot
Match: F4KGE8 (Protein GRAVITROPIC IN THE LIGHT 1 OS=Arabidopsis thaliana OX=3702 GN=GIL1 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 2.9e-07
Identity = 55/183 (30.05%), Postives = 74/183 (40.44%), Query Frame = 0

Query: 276 QSKNPRISLAFHLEALLNRAFFEDFESIGF-QKNSPNQILNPSDRTESNIAAFGLLHRLT 335
           QS  P     F LE+ + R  F+ F+   F    S + ++NP        A F  +  + 
Sbjct: 360 QSSVPNQHAKFALESYICRKIFQGFDHETFYMDGSLSSLINPDQYRRDCFAQFKDMKAMD 419

Query: 336 WEEVLS-KGTRHFSEEFSRFCDRKMSDIV------------AMLEWNRAWPEPLLQ---A 395
             E+L    T H    F +FC +K   I+               E   A   P  Q    
Sbjct: 420 PMELLGILPTCH----FGKFCSKKYLSIIHQKMEESLFGDSEQRELVVAGNHPRSQFYGE 479

Query: 396 FFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDGVYMEDIA--GDETAPA--TVRIMVS 438
           F   AK+VWL+HLLA ++ PS   F  + GA F   YME +    D   PA   V   V 
Sbjct: 480 FLGLAKAVWLLHLLAFSLDPSPSHFEANRGAEFHSQYMESVVRFSDGRVPAGQVVGFPVC 538

BLAST of MC10g1194 vs. NCBI nr
Match: XP_022152726.1 (IRK-interacting protein [Momordica charantia])

HSP 1 Score: 920 bits (2377), Expect = 0.0
Identity = 456/456 (100.00%), Postives = 456/456 (100.00%), Query Frame = 0

Query: 1   MKNITPSSPSPLHHYHSPHFFPIIQDSERTEEEDEFTNRTDAGAKHHPTPLHHHSHGGDG 60
           MKNITPSSPSPLHHYHSPHFFPIIQDSERTEEEDEFTNRTDAGAKHHPTPLHHHSHGGDG
Sbjct: 1   MKNITPSSPSPLHHYHSPHFFPIIQDSERTEEEDEFTNRTDAGAKHHPTPLHHHSHGGDG 60

Query: 61  VLPKRCSKKRPESAGYVRGGGGAVSCSKCRPHAREKFSVVPLDNNGVAKQSFSMASPNGI 120
           VLPKRCSKKRPESAGYVRGGGGAVSCSKCRPHAREKFSVVPLDNNGVAKQSFSMASPNGI
Sbjct: 61  VLPKRCSKKRPESAGYVRGGGGAVSCSKCRPHAREKFSVVPLDNNGVAKQSFSMASPNGI 120

Query: 121 FKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVLEASRLKYS 180
           FKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVLEASRLKYS
Sbjct: 121 FKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVLEASRLKYS 180

Query: 181 MAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLISDSVSDKIIENFLASVSESRSS 240
           MAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLISDSVSDKIIENFLASVSESRSS
Sbjct: 181 MAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLISDSVSDKIIENFLASVSESRSS 240

Query: 241 VRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEALLNRAFFEDF 300
           VRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEALLNRAFFEDF
Sbjct: 241 VRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEALLNRAFFEDF 300

Query: 301 ESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEEFSRFCDRKMSD 360
           ESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEEFSRFCDRKMSD
Sbjct: 301 ESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEEFSRFCDRKMSD 360

Query: 361 IVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDGVYMEDIA 420
           IVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDGVYMEDIA
Sbjct: 361 IVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDGVYMEDIA 420

Query: 421 GDETAPATVRIMVSPGFYVFDNLIKCKVLCRYHHQS 456
           GDETAPATVRIMVSPGFYVFDNLIKCKVLCRYHHQS
Sbjct: 421 GDETAPATVRIMVSPGFYVFDNLIKCKVLCRYHHQS 456

BLAST of MC10g1194 vs. NCBI nr
Match: XP_038876802.1 (IRK-interacting protein-like [Benincasa hispida])

HSP 1 Score: 739 bits (1909), Expect = 2.50e-266
Identity = 386/463 (83.37%), Postives = 408/463 (88.12%), Query Frame = 0

Query: 1   MKNITPSSPSPLHHYHSPHFFPIIQDSERTEEEDEFTNR------TDAGAKHHPTPLHHH 60
           MKNITPSS        SP F   I  +ERTEEE++  NR      T AGAKHHPTPLHHH
Sbjct: 1   MKNITPSS--------SP-FCSFIHKTERTEEEEDEFNRATPSSGTYAGAKHHPTPLHHH 60

Query: 61  SHGGDGVLPKRCSKKRPESAGYVRGGGGAVSCSKCRPHAREKFSVVPLDNNGVAKQSFSM 120
             GGDG+LPKR +KKRPESA  V   GG+VSCSKCRPHAREKFSVVPLDNNGVAKQ FS+
Sbjct: 61  --GGDGILPKRSTKKRPESAASVS--GGSVSCSKCRPHAREKFSVVPLDNNGVAKQFFSI 120

Query: 121 ASPNGIFKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVLEA 180
           ASPNGI KSIVSSLTR+SPKSMNE  DWAAREEHWKIALGEISHKLIQATRKRDEA+LEA
Sbjct: 121 ASPNGILKSIVSSLTRKSPKSMNEGTDWAAREEHWKIALGEISHKLIQATRKRDEAILEA 180

Query: 181 SRLKYSMAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLI-SDSVSDKIIENFLAS 240
           SRLKYSMAELE KLNKLEIYCH+LKS LDECA+NPRK K EVL  SDS+SDKIIENFL+S
Sbjct: 181 SRLKYSMAELEKKLNKLEIYCHSLKSELDECASNPRKPKQEVLTNSDSISDKIIENFLSS 240

Query: 241 VSESRSSVRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEALLN 300
           VSESRSSVRQLSRSL MQLR MGGKI+ER+QFLLQ +EIKIP SKNPR SL FHLEA+LN
Sbjct: 241 VSESRSSVRQLSRSLAMQLRQMGGKIYERIQFLLQSHEIKIPLSKNPRTSLIFHLEAILN 300

Query: 301 RAFFEDFESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEEFSRF 360
           RAFFEDFESIGFQKNSPNQILNPSDRTESNIA+F  LHRL+WEEVL+KGTRHFSE+FSRF
Sbjct: 301 RAFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLNKGTRHFSEDFSRF 360

Query: 361 CDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDG 420
           CDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSG RFDG
Sbjct: 361 CDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRFDG 420

Query: 421 VYMEDIAGD---ETAPATVRIMVSPGFYVFDNLIKCKVLCRYH 453
           VYMEDIAGD   E +PA VRIMVSPGFYVFDNLIKCKVLCRYH
Sbjct: 421 VYMEDIAGDKARELSPAAVRIMVSPGFYVFDNLIKCKVLCRYH 450

BLAST of MC10g1194 vs. NCBI nr
Match: XP_022923173.1 (IRK-interacting protein-like [Cucurbita moschata])

HSP 1 Score: 720 bits (1858), Expect = 2.19e-258
Identity = 367/462 (79.44%), Postives = 396/462 (85.71%), Query Frame = 0

Query: 1   MKNITPSSPSPLHHYHSPHFFPIIQDSERTEEEDEFTNRT-----DAGAKHHPTPLHHHS 60
           MKNITPS   PLHHY S  FF I+ +SERTEEEDEF   T     DAGAKHHPTPLH HS
Sbjct: 1   MKNITPSPSPPLHHYQSSPFFSIVPNSERTEEEDEFNRATPGSCTDAGAKHHPTPLHLHS 60

Query: 61  HGGDGVLPKRCSKKRPESAGYVRGGGGAVSCSKCRPHAREKFSVVPLDNNGVAKQSFSMA 120
           HGGD +LPKR +KKR E    V GGGG+VSCSKCRPHAREKFSVVP+D NGVAKQ FSMA
Sbjct: 61  HGGDEILPKRSTKKRLEPVASV-GGGGSVSCSKCRPHAREKFSVVPVDYNGVAKQFFSMA 120

Query: 121 SPNGIFKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVLEAS 180
           SPNGI KSIVSSLT +SPKSM E  DWA REE WKIA GE+SHKLI+ATRKRDEA+LEAS
Sbjct: 121 SPNGILKSIVSSLTGKSPKSMKEGADWAVREEQWKIAFGEVSHKLIEATRKRDEAILEAS 180

Query: 181 RLKYSMAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLI-SDSVSDKIIENFLASV 240
           RLKYSMAELE KLNKLEIYCH+LKS L +C +NPR    EV   SDS+SDKIIENFL+SV
Sbjct: 181 RLKYSMAELEKKLNKLEIYCHSLKSELYKCVSNPRNPNLEVPTNSDSISDKIIENFLSSV 240

Query: 241 SESRSSVRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEALLNR 300
           SESRSSVRQLSRSL MQL H+GGKI+ER+QFLL+ +EIKI  SKN + SL FHLEA+LNR
Sbjct: 241 SESRSSVRQLSRSLAMQLHHIGGKIYERIQFLLRSHEIKISLSKNSKTSLIFHLEAILNR 300

Query: 301 AFFEDFESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEEFSRFC 360
           AFFEDFESIGFQKNSPNQILNPSDRTESNIA+F  LHRL+WEEVL KGTRHFSE+FSRFC
Sbjct: 301 AFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLGKGTRHFSEDFSRFC 360

Query: 361 DRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDGV 420
           DRKMSDI+AMLEWNRAWPEPLLQAFF AAKSVWLVHLLA AVHPSLPIFRVDSG RFDGV
Sbjct: 361 DRKMSDIIAMLEWNRAWPEPLLQAFFMAAKSVWLVHLLANAVHPSLPIFRVDSGVRFDGV 420

Query: 421 YMEDIAGD---ETAPATVRIMVSPGFYVFDNLIKCKVLCRYH 453
           YMEDIAG+   E APATVRIMV+PGFY FD LIKCKV+CRYH
Sbjct: 421 YMEDIAGEKARELAPATVRIMVAPGFYAFDGLIKCKVMCRYH 461

BLAST of MC10g1194 vs. NCBI nr
Match: KAG6577593.1 (IRK-interacting protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 719 bits (1857), Expect = 3.22e-258
Identity = 369/463 (79.70%), Postives = 398/463 (85.96%), Query Frame = 0

Query: 1   MKNITPSSPSP-LHHYHSPHFFPIIQDSERTEEEDEFTNRT-----DAGAKHHPTPLHHH 60
           MKNITPSSPSP LHHY S  FF I+ +SERTEEEDEF   T     DAGAKHHPTPLH H
Sbjct: 1   MKNITPSSPSPPLHHYQSSPFFSIVPNSERTEEEDEFNRATPSSCTDAGAKHHPTPLHLH 60

Query: 61  SHGGDGVLPKRCSKKRPESAGYVRGGGGAVSCSKCRPHAREKFSVVPLDNNGVAKQSFSM 120
           SHGGD +LPKR +KKR E    V GGGG+VSCSKCRPHAREKFSVVP+D NGVAKQ FSM
Sbjct: 61  SHGGDEILPKRSTKKRLEPVASV-GGGGSVSCSKCRPHAREKFSVVPVDYNGVAKQFFSM 120

Query: 121 ASPNGIFKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVLEA 180
           ASPNGI KSIVSSLT +SPKSM E  DWA REE WKIA  E+SHKLI+ATRKRDEA+LEA
Sbjct: 121 ASPNGILKSIVSSLTGKSPKSMKEGADWAVREEQWKIAFDEVSHKLIEATRKRDEAILEA 180

Query: 181 SRLKYSMAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLI-SDSVSDKIIENFLAS 240
           SRLKYSMAELE KLNKLEIYCH+LKS L +C +NPR    EV   SDS+SDKIIENFL+S
Sbjct: 181 SRLKYSMAELEKKLNKLEIYCHSLKSELYKCVSNPRNPNLEVPTNSDSISDKIIENFLSS 240

Query: 241 VSESRSSVRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEALLN 300
           VSESRSSVRQLSRSL MQL H+GGKI+ER+QFLL+ +EIKI  SKN + SL FHLEA+LN
Sbjct: 241 VSESRSSVRQLSRSLAMQLHHIGGKIYERIQFLLRSHEIKISLSKNSKTSLIFHLEAILN 300

Query: 301 RAFFEDFESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEEFSRF 360
           RAFFEDFESIGFQKNSPNQILNPSDRTESNIA+F  LHRL+WEEVL KGTRHFSE+FSRF
Sbjct: 301 RAFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLGKGTRHFSEDFSRF 360

Query: 361 CDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDG 420
           CDRKMSDI+AMLEWNRAWPEPLLQAFF AAKSVWLVHLLA AVHPSLPIFRVDSG RFDG
Sbjct: 361 CDRKMSDIIAMLEWNRAWPEPLLQAFFMAAKSVWLVHLLANAVHPSLPIFRVDSGVRFDG 420

Query: 421 VYMEDIAGD---ETAPATVRIMVSPGFYVFDNLIKCKVLCRYH 453
           VYMEDIAG+   E APATVRIMV+PGFY FD LIKCKV+CRYH
Sbjct: 421 VYMEDIAGEKARELAPATVRIMVAPGFYAFDGLIKCKVMCRYH 462

BLAST of MC10g1194 vs. NCBI nr
Match: XP_023552161.1 (IRK-interacting protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 718 bits (1853), Expect = 1.36e-257
Identity = 370/464 (79.74%), Postives = 400/464 (86.21%), Query Frame = 0

Query: 1   MKNITPSS-PSP-LHHYHSPHFFPIIQDSERTEEEDEFTNRT-----DAGAKHHPTPLHH 60
           MKNITPSS PSP LHHY S  FF I+ +SERTEEEDEF   T     DAGAKHHPTPLH 
Sbjct: 1   MKNITPSSSPSPPLHHYQSSPFFSIVPNSERTEEEDEFNRATPSSCTDAGAKHHPTPLHL 60

Query: 61  HSHGGDGVLPKRCSKKRPESAGYVRGGGGAVSCSKCRPHAREKFSVVPLDNNGVAKQSFS 120
           HSHGGD +LPKR +KKR ES   + GGGG+VSCSKCRPHAREKFSVVP+D NGVAKQ FS
Sbjct: 61  HSHGGDEILPKRSTKKRLESVASI-GGGGSVSCSKCRPHAREKFSVVPVDYNGVAKQFFS 120

Query: 121 MASPNGIFKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVLE 180
           MASPNGI KSIVSSLT +SPKSM E  DWA REE WKIA GE+SHKLI+ATRKRDEA+LE
Sbjct: 121 MASPNGILKSIVSSLTGKSPKSMKEGGDWAVREEQWKIAFGEVSHKLIEATRKRDEAILE 180

Query: 181 ASRLKYSMAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLI-SDSVSDKIIENFLA 240
           ASRLKYSMAELE KLNKLEIYCH+LKS L +C +NPR    EV   SDS+SDKIIENFL+
Sbjct: 181 ASRLKYSMAELEKKLNKLEIYCHSLKSELYKCVSNPRNPNLEVPTNSDSISDKIIENFLS 240

Query: 241 SVSESRSSVRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEALL 300
           SVSESRSSVRQLSRSL MQL H+GGKI+ER+QFLL+ +EIKI  SKN + SL FHLEA+L
Sbjct: 241 SVSESRSSVRQLSRSLAMQLHHIGGKIYERIQFLLRSHEIKISLSKNSKTSLIFHLEAIL 300

Query: 301 NRAFFEDFESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEEFSR 360
           NRAFFEDFESIGFQKNSPNQILNPSDRTESNIA+F  LHRL+WEEVL KGTRHFSE+FSR
Sbjct: 301 NRAFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLGKGTRHFSEDFSR 360

Query: 361 FCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFD 420
           FCDRKMSDI+AMLEWNRAWPEPLLQAFF AAKSVWLVHLLA AVHPSLPIFRVDSG RFD
Sbjct: 361 FCDRKMSDIIAMLEWNRAWPEPLLQAFFMAAKSVWLVHLLANAVHPSLPIFRVDSGVRFD 420

Query: 421 GVYMEDIAGD---ETAPATVRIMVSPGFYVFDNLIKCKVLCRYH 453
           GVYMEDIAG+   E APATVRIMV+PGFY FD LIKCKV+CRYH
Sbjct: 421 GVYMEDIAGEKARELAPATVRIMVAPGFYAFDGLIKCKVMCRYH 463

BLAST of MC10g1194 vs. ExPASy TrEMBL
Match: A0A6J1DIL9 (IRK-interacting protein OS=Momordica charantia OX=3673 GN=LOC111020381 PE=4 SV=1)

HSP 1 Score: 920 bits (2377), Expect = 0.0
Identity = 456/456 (100.00%), Postives = 456/456 (100.00%), Query Frame = 0

Query: 1   MKNITPSSPSPLHHYHSPHFFPIIQDSERTEEEDEFTNRTDAGAKHHPTPLHHHSHGGDG 60
           MKNITPSSPSPLHHYHSPHFFPIIQDSERTEEEDEFTNRTDAGAKHHPTPLHHHSHGGDG
Sbjct: 1   MKNITPSSPSPLHHYHSPHFFPIIQDSERTEEEDEFTNRTDAGAKHHPTPLHHHSHGGDG 60

Query: 61  VLPKRCSKKRPESAGYVRGGGGAVSCSKCRPHAREKFSVVPLDNNGVAKQSFSMASPNGI 120
           VLPKRCSKKRPESAGYVRGGGGAVSCSKCRPHAREKFSVVPLDNNGVAKQSFSMASPNGI
Sbjct: 61  VLPKRCSKKRPESAGYVRGGGGAVSCSKCRPHAREKFSVVPLDNNGVAKQSFSMASPNGI 120

Query: 121 FKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVLEASRLKYS 180
           FKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVLEASRLKYS
Sbjct: 121 FKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVLEASRLKYS 180

Query: 181 MAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLISDSVSDKIIENFLASVSESRSS 240
           MAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLISDSVSDKIIENFLASVSESRSS
Sbjct: 181 MAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLISDSVSDKIIENFLASVSESRSS 240

Query: 241 VRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEALLNRAFFEDF 300
           VRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEALLNRAFFEDF
Sbjct: 241 VRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEALLNRAFFEDF 300

Query: 301 ESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEEFSRFCDRKMSD 360
           ESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEEFSRFCDRKMSD
Sbjct: 301 ESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEEFSRFCDRKMSD 360

Query: 361 IVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDGVYMEDIA 420
           IVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDGVYMEDIA
Sbjct: 361 IVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDGVYMEDIA 420

Query: 421 GDETAPATVRIMVSPGFYVFDNLIKCKVLCRYHHQS 456
           GDETAPATVRIMVSPGFYVFDNLIKCKVLCRYHHQS
Sbjct: 421 GDETAPATVRIMVSPGFYVFDNLIKCKVLCRYHHQS 456

BLAST of MC10g1194 vs. ExPASy TrEMBL
Match: A0A6J1E8U8 (IRK-interacting protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430947 PE=4 SV=1)

HSP 1 Score: 720 bits (1858), Expect = 1.06e-258
Identity = 367/462 (79.44%), Postives = 396/462 (85.71%), Query Frame = 0

Query: 1   MKNITPSSPSPLHHYHSPHFFPIIQDSERTEEEDEFTNRT-----DAGAKHHPTPLHHHS 60
           MKNITPS   PLHHY S  FF I+ +SERTEEEDEF   T     DAGAKHHPTPLH HS
Sbjct: 1   MKNITPSPSPPLHHYQSSPFFSIVPNSERTEEEDEFNRATPGSCTDAGAKHHPTPLHLHS 60

Query: 61  HGGDGVLPKRCSKKRPESAGYVRGGGGAVSCSKCRPHAREKFSVVPLDNNGVAKQSFSMA 120
           HGGD +LPKR +KKR E    V GGGG+VSCSKCRPHAREKFSVVP+D NGVAKQ FSMA
Sbjct: 61  HGGDEILPKRSTKKRLEPVASV-GGGGSVSCSKCRPHAREKFSVVPVDYNGVAKQFFSMA 120

Query: 121 SPNGIFKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVLEAS 180
           SPNGI KSIVSSLT +SPKSM E  DWA REE WKIA GE+SHKLI+ATRKRDEA+LEAS
Sbjct: 121 SPNGILKSIVSSLTGKSPKSMKEGADWAVREEQWKIAFGEVSHKLIEATRKRDEAILEAS 180

Query: 181 RLKYSMAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLI-SDSVSDKIIENFLASV 240
           RLKYSMAELE KLNKLEIYCH+LKS L +C +NPR    EV   SDS+SDKIIENFL+SV
Sbjct: 181 RLKYSMAELEKKLNKLEIYCHSLKSELYKCVSNPRNPNLEVPTNSDSISDKIIENFLSSV 240

Query: 241 SESRSSVRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEALLNR 300
           SESRSSVRQLSRSL MQL H+GGKI+ER+QFLL+ +EIKI  SKN + SL FHLEA+LNR
Sbjct: 241 SESRSSVRQLSRSLAMQLHHIGGKIYERIQFLLRSHEIKISLSKNSKTSLIFHLEAILNR 300

Query: 301 AFFEDFESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEEFSRFC 360
           AFFEDFESIGFQKNSPNQILNPSDRTESNIA+F  LHRL+WEEVL KGTRHFSE+FSRFC
Sbjct: 301 AFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLGKGTRHFSEDFSRFC 360

Query: 361 DRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDGV 420
           DRKMSDI+AMLEWNRAWPEPLLQAFF AAKSVWLVHLLA AVHPSLPIFRVDSG RFDGV
Sbjct: 361 DRKMSDIIAMLEWNRAWPEPLLQAFFMAAKSVWLVHLLANAVHPSLPIFRVDSGVRFDGV 420

Query: 421 YMEDIAGD---ETAPATVRIMVSPGFYVFDNLIKCKVLCRYH 453
           YMEDIAG+   E APATVRIMV+PGFY FD LIKCKV+CRYH
Sbjct: 421 YMEDIAGEKARELAPATVRIMVAPGFYAFDGLIKCKVMCRYH 461

BLAST of MC10g1194 vs. ExPASy TrEMBL
Match: A0A1S3BLQ8 (IRK-interacting protein-like OS=Cucumis melo OX=3656 GN=LOC103490937 PE=4 SV=1)

HSP 1 Score: 717 bits (1850), Expect = 1.25e-257
Identity = 376/466 (80.69%), Postives = 402/466 (86.27%), Query Frame = 0

Query: 1   MKNITPSSPSPLHHYHSPHFFPIIQDSERTEEE----DEFTNRT-----DAGAKHHPTPL 60
           MKNITPSSP          FF II  ++RTEEE    DEF   T     DAGAKHHPTPL
Sbjct: 1   MKNITPSSP----------FFSIIHKTDRTEEEEEEDDEFNKATPCSGTDAGAKHHPTPL 60

Query: 61  HHHSHGGDGVLPKRCSKKRPESAGYVRGGGGAVSCSKCRPHAREKFSVVPLDNNGVAKQS 120
           H   HG DGVLPKR +KK P+SA  V    G+VSCSKCRPHAREKFSVVPLDNNGVAKQ 
Sbjct: 61  H--LHGSDGVLPKRSTKKHPDSAASVS--TGSVSCSKCRPHAREKFSVVPLDNNGVAKQF 120

Query: 121 FSMASPNGIFKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAV 180
           FSMASPNG+ KSIVSSLTR+SPK +NE  D AAREEHWKIALGEISHKLIQATRKRDEA+
Sbjct: 121 FSMASPNGMLKSIVSSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAI 180

Query: 181 LEASRLKYSMAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLI-SDSVSDKIIENF 240
           LEASRLKYSMAELE KLNKLEIYCHTLKS LDECA+NPRK K EV   SDS+SDKIIENF
Sbjct: 181 LEASRLKYSMAELEKKLNKLEIYCHTLKSELDECASNPRKPKSEVQKNSDSISDKIIENF 240

Query: 241 LASVSESRSSVRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEA 300
           L+SVSESRSSVRQLSRSL MQLR +GGK++ER+QFLLQ ++IKIP SKN + SL FHLEA
Sbjct: 241 LSSVSESRSSVRQLSRSLAMQLRQIGGKVYERIQFLLQSHDIKIPLSKNLKTSLIFHLEA 300

Query: 301 LLNRAFFEDFESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEEF 360
           +LNRAFFEDFE+IGFQKNSPNQILNPSDRTESNIA+F  LHRL+WEEVLSKGTRHFSE+F
Sbjct: 301 ILNRAFFEDFETIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLSKGTRHFSEDF 360

Query: 361 SRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGAR 420
           SRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSG R
Sbjct: 361 SRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVR 420

Query: 421 FDGVYMEDIAGD---ETAPATVRIMVSPGFYVFDNLIKCKVLCRYH 453
           FDGVYMEDIAG+   E APATVRIMVSPGFYVFDNLIKCKV+CRYH
Sbjct: 421 FDGVYMEDIAGEKARELAPATVRIMVSPGFYVFDNLIKCKVVCRYH 452

BLAST of MC10g1194 vs. ExPASy TrEMBL
Match: A0A0A0L1R1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G036460 PE=4 SV=1)

HSP 1 Score: 712 bits (1837), Expect = 1.19e-255
Identity = 375/465 (80.65%), Postives = 405/465 (87.10%), Query Frame = 0

Query: 1   MKNITPSSPSPLHHYHSPHFFPIIQDSERTEEE---DEF-----TNRTDAGAKHHPTPLH 60
           MKNITPSS        SP F  II +++RTEEE   DEF     T+ TDAG KHHPTPLH
Sbjct: 1   MKNITPSS--------SP-FSSIIHNTDRTEEEEEEDEFNRATPTSGTDAGPKHHPTPLH 60

Query: 61  HHSHGGDGVLPKRCSKKRPESAGYVRGGGGAVSCSKCRPHAREKFSVVPLDNNGVAKQSF 120
              +GGDGVLPKR  KK P+SA  V    G+VSCSKCRPHAREKFSVVPLDNNGVAKQ F
Sbjct: 61  --LYGGDGVLPKRSIKKHPDSAASVS--TGSVSCSKCRPHAREKFSVVPLDNNGVAKQFF 120

Query: 121 SMASPNGIFKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVL 180
           SMASPNG+ KSIVSSLTR+SPK +NE  D AAREEHWKIALGEISHKLIQATRKRDEA+L
Sbjct: 121 SMASPNGMLKSIVSSLTRKSPKPINEGTDLAAREEHWKIALGEISHKLIQATRKRDEAIL 180

Query: 181 EASRLKYSMAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLI-SDSVSDKIIENFL 240
           EASRLKYSM+ELE KLNKLEIYCHTLKS LDECA+NPRK+KPEV   SDS+SDKIIENFL
Sbjct: 181 EASRLKYSMSELEKKLNKLEIYCHTLKSELDECASNPRKTKPEVQKNSDSISDKIIENFL 240

Query: 241 ASVSESRSSVRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEAL 300
           +SVSESRSSVRQLSRSL MQLR +GGKI+ER+QFLLQ  +IKIP SKN + SL FHLEA+
Sbjct: 241 SSVSESRSSVRQLSRSLAMQLRQIGGKIYERIQFLLQSQDIKIPLSKNLKTSLIFHLEAI 300

Query: 301 LNRAFFEDFESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEEFS 360
           LNRAFFEDFE+IGFQKNSPNQILNPSDRTE+NIA+F  LHRL+WEEVLSKGTRHFSE+FS
Sbjct: 301 LNRAFFEDFETIGFQKNSPNQILNPSDRTEANIASFNRLHRLSWEEVLSKGTRHFSEDFS 360

Query: 361 RFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARF 420
           RFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSG RF
Sbjct: 361 RFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGVRF 420

Query: 421 DGVYMEDIAGD---ETAPATVRIMVSPGFYVFDNLIKCKVLCRYH 453
           DGVYMEDIAG+   E APATVRIM+SPGFYVFDNLIKCKV+CRYH
Sbjct: 421 DGVYMEDIAGEKARELAPATVRIMLSPGFYVFDNLIKCKVVCRYH 452

BLAST of MC10g1194 vs. ExPASy TrEMBL
Match: A0A6J1HMP3 (IRK-interacting protein-like OS=Cucurbita maxima OX=3661 GN=LOC111465023 PE=4 SV=1)

HSP 1 Score: 711 bits (1836), Expect = 2.54e-255
Identity = 367/464 (79.09%), Postives = 396/464 (85.34%), Query Frame = 0

Query: 1   MKNITPSS-PSP-LHHYHSPHFFPIIQDSERTEEEDEFTNRT-----DAGAKHHPTPLHH 60
           MKNITPSS PSP LHHY S  FF I+ +SERTEEEDEF   T     D GAKHHPTPLH 
Sbjct: 1   MKNITPSSSPSPPLHHYQSSPFFSIVPNSERTEEEDEFNRATPSSCTDDGAKHHPTPLHL 60

Query: 61  HSHGGDGVLPKRCSKKRPESAGYVRGGGGAVSCSKCRPHAREKFSVVPLDNNGVAKQSFS 120
           HSHGGD +LPKR +KKR E    V G GG+VSCSKCRPHAREKFSVVP+D NGVAKQ FS
Sbjct: 61  HSHGGDEILPKRSTKKRLEPVASV-GDGGSVSCSKCRPHAREKFSVVPVDYNGVAKQFFS 120

Query: 121 MASPNGIFKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVLE 180
           MASPNGI KSIVSS T +SPKSM E  DWA REEHWKIA GE+SHKLI+ATRKRDEA+LE
Sbjct: 121 MASPNGILKSIVSSFTGKSPKSMKEGADWAVREEHWKIAFGEVSHKLIEATRKRDEAILE 180

Query: 181 ASRLKYSMAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLI-SDSVSDKIIENFLA 240
           ASRLKYSMAELE KLNKLEIYCH+LKS L +C +NPR    EV   SDS+SDKIIENFL+
Sbjct: 181 ASRLKYSMAELEKKLNKLEIYCHSLKSELYKCVSNPRNPNLEVPTNSDSISDKIIENFLS 240

Query: 241 SVSESRSSVRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEALL 300
           SVSESRSS+RQLSRSL MQL H+G KI+ER+QFLL+ ++IKI  SKN + SL FHLEA+L
Sbjct: 241 SVSESRSSMRQLSRSLAMQLHHIGDKIYERIQFLLRSHDIKISLSKNSKTSLIFHLEAIL 300

Query: 301 NRAFFEDFESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEEFSR 360
           NRAFFEDFESIGFQKNSPNQILNPSDRTESNIA+F  LHRL+WEEVL KGTRHFSE+FSR
Sbjct: 301 NRAFFEDFESIGFQKNSPNQILNPSDRTESNIASFNRLHRLSWEEVLGKGTRHFSEDFSR 360

Query: 361 FCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFD 420
           FCDRKMSDI+AMLEWNRAWPEPLLQAFF AAKSVWLVHLLATAVHPSLPIFRVDSG RFD
Sbjct: 361 FCDRKMSDIIAMLEWNRAWPEPLLQAFFMAAKSVWLVHLLATAVHPSLPIFRVDSGVRFD 420

Query: 421 GVYMEDIAGD---ETAPATVRIMVSPGFYVFDNLIKCKVLCRYH 453
           GVYMEDIAGD   E APATVRIMV+PGFY FD LIKCKV CRYH
Sbjct: 421 GVYMEDIAGDKARELAPATVRIMVAPGFYAFDGLIKCKVTCRYH 463

BLAST of MC10g1194 vs. TAIR 10
Match: AT1G12330.1 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 12 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G12900.1); Has 249 Blast hits to 249 proteins in 27 species: Archae - 0; Bacteria - 0; Metazoa - 7; Fungi - 14; Plants - 217; Viruses - 0; Other Eukaryotes - 11 (source: NCBI BLink). )

HSP 1 Score: 464.2 bits (1193), Expect = 1.2e-130
Identity = 263/471 (55.84%), Postives = 330/471 (70.06%), Query Frame = 0

Query: 8   SPSPLHHYHSPHFFPIIQDSERTEEEDEFTNR----TDAGAKHHPTPLHH--------HS 67
           SP P  H  S HF PI++  E   +E+ + NR    +D G+   P   HH        H 
Sbjct: 12  SPVPSLH-QSLHFTPILECEEDDLQEERYKNRATPSSDGGSSATPNHQHHRRTNSNNNHQ 71

Query: 68  HGGDGVLPKRCSKKRPESAGYVRGGGGAVSCSKCRPH--AREKFSVVPLDNNGVAKQSFS 127
           H    +      + R          GGAVSC+KCRPH   R+KFSVVPL+++     SF 
Sbjct: 72  HTLTPLHHNGKPQTRKRHDDNDEDDGGAVSCNKCRPHHSHRDKFSVVPLESHN--NPSF- 131

Query: 128 MASPNGIFKSIVSSLTRRSPK------------SMNEAVDWAAREEHWKIALGEISHKLI 187
           ++SPN I KSI  SLTRRSPK            S + A D A+REE W++A+ E+SHKLI
Sbjct: 132 ISSPNLIIKSIFQSLTRRSPKPSSATAALPPRSSSSSAAD-ASREEQWRLAVAELSHKLI 191

Query: 188 QATRKRDEAVLEASRLKYSMAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLISDS 247
           QAT+K+++AV+EASRLK SMAELE KLNKLEIYCH LKSGLDEC +N ++S P  +  D 
Sbjct: 192 QATKKKEDAVIEASRLKSSMAELEKKLNKLEIYCHNLKSGLDEC-SNKKQSVP--IRKDG 251

Query: 248 VSDKIIENFLASVSESRSSVRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQ-SKNP 307
            +D+II+ FL SVSESRSS+R LSRSL  QLR +GGK++ERL  LLQP ++KI   +KNP
Sbjct: 252 FNDRIIQQFLVSVSESRSSIRALSRSLASQLRTVGGKVYERLSLLLQPFDVKINSFAKNP 311

Query: 308 RISLAFHLEALLNRAFFEDFESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLS 367
           + SL F+LEA+L+RAFFEDFE+ GFQKN   +ILNP DR ESN A+F +L  LTW+EVLS
Sbjct: 312 K-SLIFYLEAILSRAFFEDFEAPGFQKNGSTRILNPIDRCESNYASFNVLMELTWDEVLS 371

Query: 368 KGTRHFSEEFSRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSL 427
           +GT+HFSEEFSRFCDRKMSD+V+ML WNRAWPEPLLQAFF A+KSVWLVHLLA +V+P L
Sbjct: 372 RGTKHFSEEFSRFCDRKMSDVVSMLSWNRAWPEPLLQAFFGASKSVWLVHLLANSVNPGL 431

Query: 428 PIFRVDSGARFDGVYMEDIAGDETAPATVRIMVSPGFYVFDNLIKCKVLCR 452
            IFRV+   RFD +YME+  G E   + VR MV PGFYV+ +++KCKV+C+
Sbjct: 432 QIFRVEKDDRFDPIYMEE-TGGERFKSLVRAMVQPGFYVYGSVVKCKVVCK 472

BLAST of MC10g1194 vs. TAIR 10
Match: AT5G12900.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G12330.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 242.3 bits (617), Expect = 7.7e-64
Identity = 135/351 (38.46%), Postives = 203/351 (57.83%), Query Frame = 0

Query: 118 NGIFKSIVSSLTRRSPKSMNEAVDWAAREEHWKIALGEISHKLIQATRKRDEAVLEASRL 177
           N IF S   S+T +S +      D  +  E       ++  +L++A R RD A+ + S +
Sbjct: 222 NSIFNS--PSITEKSEEVSEVLKDSGSGVE-------KLKRELMEANRSRDAALTQVSEM 281

Query: 178 KYSMAELENKLNKLEIYCHTLKSGLDEC----------AANPRKSKPEVLISDSVSDKII 237
           K S+ EL  KL  LE YC  LK  L E             +  K   E+ +S+ V   ++
Sbjct: 282 KSSLGELSEKLQYLESYCDNLKKALREATEVVSQENSGGRSSGKKNSEMPVSEEV---MV 341

Query: 238 ENFLASVSESRSSVRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFH 297
           E FL  VSE+R S++Q  ++L  ++      +   +  LLQP+ +    SK  +I + +H
Sbjct: 342 EGFLQIVSEARLSIKQFLKTLVSEIDEEDSTLIGNINTLLQPHNLSF-TSKYSKI-IQYH 401

Query: 298 LEALLNRAFFEDFESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFS 357
           LEA+++++ ++DFE+  FQKN   ++L+P    ++N ++F  L  L+W EVL KGT+++S
Sbjct: 402 LEAIISQSVYQDFENCVFQKNGKPKLLDPEQDRQANFSSFASLRNLSWNEVLKKGTKYYS 461

Query: 358 EEFSRFCDRKMSDIVAMLEWNRAWPEPLLQAFFAAAKSVWLVHLLATAVHPSLPIFRVDS 417
           +EFSRFCD KMS I+  L W R W E +LQAFF AAK VWL+HLLA + +P+L I RV+ 
Sbjct: 462 DEFSRFCDEKMSLIITTLNWTRPWSEQMLQAFFVAAKCVWLLHLLAFSFNPALGILRVEE 521

Query: 418 GARFDGVYMEDIAGD------ETAPATVRIMVSPGFYVFDNLIKCKVLCRY 453
              F+  +MED+  D         PA V++MV PGFYV D +++CKVLCRY
Sbjct: 522 NREFESSFMEDMGADRQRSALSRGPARVKVMVMPGFYVLDRVLRCKVLCRY 558

BLAST of MC10g1194 vs. TAIR 10
Match: AT2G45260.1 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 60.1 bits (144), Expect = 5.4e-09
Identity = 58/245 (23.67%), Postives = 102/245 (41.63%), Query Frame = 0

Query: 224 DKIIENFLASVSESRSSVRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRIS 283
           D   E ++++   +  +V   S+ L   ++  G  +      +    E  +  +K P   
Sbjct: 181 DLTTELYVSTYEAAAKAVHDFSKPLINMMKAAGWDLDSAANSI----EPDVVYAKRPHKK 240

Query: 284 LAFHLEALLNRAFFEDFESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGT 343
            AF  E+ + +  F  F+   F  NS +  +  +D T++    F  L  +   + L  GT
Sbjct: 241 YAF--ESYICQRMFSGFQQKNFSVNSESAAVMANDDTDTFFRQFLALKDMDPLDAL--GT 300

Query: 344 RHFSEEFSRFCDRKMSDIV------------AMLEWNRAWPEP---LLQAFFAAAKSVWL 403
              S  F  FC  K   +V               ++      P     QAF   AKS+W+
Sbjct: 301 NPDS-NFGIFCRSKYLLLVHPKMEASFFGNLDQRDYVTGGGHPRTAFYQAFLKLAKSIWI 360

Query: 404 VHLLATAVHPSLPIFRVDSGARFDGVYMED-----IAGDETAPATVRIMVSPGFYVFDNL 449
           +H LA +  P+  IF+V  G+ F   YME      +  ++     V +MV PGF++  ++
Sbjct: 361 LHRLAYSFDPAAKIFQVKKGSEFSDSYMESVVKNIVVDEKEENPRVGLMVMPGFWIGGSV 416

BLAST of MC10g1194 vs. TAIR 10
Match: AT3G60680.1 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 60.1 bits (144), Expect = 5.4e-09
Identity = 79/356 (22.19%), Postives = 144/356 (40.45%), Query Frame = 0

Query: 121 FKSIVSSLTRRSPKSMNEAVDWAAREEHWKI-ALGEISHKLIQATRKRDEAVLEASRLKY 180
           +++   SL   S  ++   ++   +E   K+ AL  +S++L        +A ++A  L+ 
Sbjct: 172 YRNYRQSLDFESDLAIGSCLESRVQENQSKLRALETVSNRL--------QAEMDAKDLQ- 231

Query: 181 SMAELENKLNKLEIYCHTLKSGLDECAANPRKSKPEVLISDSVSDKIIENFLASVSESRS 240
            +  L NKL +++     L   L         S  +VL+S  V + ++ +   +  +   
Sbjct: 232 -VWSLRNKLGEIQKSTSKLSKRLSS------NSSLDVLLSVRVFESLLYDAFKATQKFTK 291

Query: 241 SVRQLSRSLTMQLRHMGGKIHERLQFLLQPNEIKIPQSKNPRISLAFHLEALLNRAFFED 300
            + +L       L  +   +H          E+   + ++ R +L     + +    F  
Sbjct: 292 ILIELMEKAGWDLDLVAKSVHP---------EVDYAKERHNRYALL----SYVCLGMFRG 351

Query: 301 FESIGFQKNSPNQILNPSDRTESNIAAFGLLHRLTWEEVLSKGTRHFSEE----FSRFCD 360
           F+  GF        LN +D  ES  ++     R   + V S        +    FSRFCD
Sbjct: 352 FDGEGFD-------LNENDYEESERSSVDSSLRELMQHVSSNPMELLDRDKDCAFSRFCD 411

Query: 361 RK---------MSDIVAMLEWNRA----WP--EPLLQAFFAAAKSVWLVHLLATAVHPSL 420
           +K          S I + ++ N A    W       ++F   A S+W +H LA +  P++
Sbjct: 412 KKYHELIHPNMASSIFSNMDENEAVLSSWRSLSTFYESFVTMASSIWTLHKLALSFDPAV 471

Query: 421 PIFRVDSGARFDGVYMEDIA--------GDETAPATVRIMVSPGFYVFDNLIKCKV 449
            IF+V+SG  F  V+ME++               A V   V PGF +   +I+C+V
Sbjct: 472 EIFQVESGVEFSIVFMENVLKRKQDKKFSMSPTRAKVGFTVVPGFKIGCTVIQCQV 491

BLAST of MC10g1194 vs. TAIR 10
Match: AT5G58960.1 (Plant protein of unknown function (DUF641) )

HSP 1 Score: 58.2 bits (139), Expect = 2.1e-08
Identity = 55/183 (30.05%), Postives = 74/183 (40.44%), Query Frame = 0

Query: 276 QSKNPRISLAFHLEALLNRAFFEDFESIGF-QKNSPNQILNPSDRTESNIAAFGLLHRLT 335
           QS  P     F LE+ + R  F+ F+   F    S + ++NP        A F  +  + 
Sbjct: 360 QSSVPNQHAKFALESYICRKIFQGFDHETFYMDGSLSSLINPDQYRRDCFAQFKDMKAMD 419

Query: 336 WEEVLS-KGTRHFSEEFSRFCDRKMSDIV------------AMLEWNRAWPEPLLQ---A 395
             E+L    T H    F +FC +K   I+               E   A   P  Q    
Sbjct: 420 PMELLGILPTCH----FGKFCSKKYLSIIHQKMEESLFGDSEQRELVVAGNHPRSQFYGE 479

Query: 396 FFAAAKSVWLVHLLATAVHPSLPIFRVDSGARFDGVYMEDIA--GDETAPA--TVRIMVS 438
           F   AK+VWL+HLLA ++ PS   F  + GA F   YME +    D   PA   V   V 
Sbjct: 480 FLGLAKAVWLLHLLAFSLDPSPSHFEANRGAEFHSQYMESVVRFSDGRVPAGQVVGFPVC 538

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LXU91.1e-6238.46IRK-interacting protein OS=Arabidopsis thaliana OX=3702 GN=IRKI PE=1 SV=1[more]
F4KGE82.9e-0730.05Protein GRAVITROPIC IN THE LIGHT 1 OS=Arabidopsis thaliana OX=3702 GN=GIL1 PE=1 ... [more]
Match NameE-valueIdentityDescription
XP_022152726.10.0100.00IRK-interacting protein [Momordica charantia][more]
XP_038876802.12.50e-26683.37IRK-interacting protein-like [Benincasa hispida][more]
XP_022923173.12.19e-25879.44IRK-interacting protein-like [Cucurbita moschata][more]
KAG6577593.13.22e-25879.70IRK-interacting protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023552161.11.36e-25779.74IRK-interacting protein-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1DIL90.0100.00IRK-interacting protein OS=Momordica charantia OX=3673 GN=LOC111020381 PE=4 SV=1[more]
A0A6J1E8U81.06e-25879.44IRK-interacting protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430947 PE=4 ... [more]
A0A1S3BLQ81.25e-25780.69IRK-interacting protein-like OS=Cucumis melo OX=3656 GN=LOC103490937 PE=4 SV=1[more]
A0A0A0L1R11.19e-25580.65Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G036460 PE=4 SV=1[more]
A0A6J1HMP32.54e-25579.09IRK-interacting protein-like OS=Cucurbita maxima OX=3661 GN=LOC111465023 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT1G12330.11.2e-13055.84unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplas... [more]
AT5G12900.17.7e-6438.46unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT2G45260.15.4e-0923.67Plant protein of unknown function (DUF641) [more]
AT3G60680.15.4e-0922.19Plant protein of unknown function (DUF641) [more]
AT5G58960.12.1e-0830.05Plant protein of unknown function (DUF641) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 167..194
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..72
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 29..88
NoneNo IPR availablePANTHERPTHR31029:SF4CYCLIN-DEPENDENT KINASE-LIKE PROTEINcoord: 13..454
IPR042316IRK-interacting protein-likePANTHERPTHR31029CYCLIN-DEPENDENT KINASE-LIKE PROTEINcoord: 13..454

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g1194.1MC10g1194.1mRNA