MC10g1192 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g1192
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionmethylsterol monooxygenase 1-1-like
LocationMC10: 15050223 .. 15055255 (+)
RNA-Seq ExpressionMC10g1192
SyntenyMC10g1192
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCACAACTTTTTAATCTAAAATAGATATCTTAACCTCGATTGATTTAGATTTATTACGAAAGAGAGAGTCGGGAATTGGTGTGTTACTAAGTTGAGTTGGGAGAGAGAAGGCACGTGAAAGATTTGGAAGAGAGAGAGTGGAGAGAGAAGGCATGTGAAAGATTTGGAAGGGAGTGACAGACGGCAATGGAGTGGACAGCATTCTCCTCCGCACATATATATAAACAAACATTGAATCCACAGAATTTCACGGCCTTCCTCTTGTTTTCTTCTCTTCTCTGTCCTTCAAAATTTCCCGCATAATTTTAACTTTCTGGGTTTCTCTCCGATTCCCGATTTCGATTCTCTTCTTCCATTCATATTTCATCCCTTTTGGCCGCTTCTTCCTCCGCCGGCGGCGTAAATTCCATCTGGGTTCTCCTCCGCCGCTCCCCAATGTTGCCGTTTCAGTCCCTCCACGAAGCCCAGTCGGCGCTTGGCCGGAATCTCACCTTCGCCGAGACCCTCTGGTTCAATTACTCCGCCGATAAGTCCGATTACTTCCTCTATTGCCACAACATTCTCTTCCTCTTCCTCATTTTCTCCTTTATTCCCCTCCCCCTCGTCTTCCTCGAGATTCTTCACGCAGCCGGAATTTATAAGTACAAAATTCAGCCAAAAGTCCGATTGCCCTTTTCCGAGATGTTCCGTTGCTACAGAGATGTCATGCGGATGTTCTTCCTCATCGTTGGCCCTCTCCAACTCATCTCCTTCCCTTGCATTAAGGTAAATCTGGGGTTCCCATTTTTATCTTTTAATTCATCGATTCTGCTATGGAGTTTACGAATCAATTCATTTTCATTCGTTTGTTAATTTGTTTTGTTGGGTTTTGTTTTGGTAGATGATTGGGATCAGGACAGGGTTGCCATTGCCTTCAGGATGGGAAATTCTTTCCCAATTACTGGTTTATTTCATGATCGAGGATTATACAAACTATTGGGTTCATAGATTCCTTCACTGCAAATGGGGGTACGAGAAAATCCATCGGGTTCACCATGAATACACAGCTCCTATAGGATTTGCAGCACCATATGCTCATTGGGCTGAGGTTTTGGTTCTTGGAATTCCCTCTTTTCTTGGTCCAGCCATGGTTCCTGGTCATATGATCACCTTCTGGTTGTGGATTGCTTTAAGGCAGATTGAGGCCATTGAAACGCATAGTGGGTAAGCATGCTAAAGCTCTCTCTCTCTCTCGTCTGTTTATCTCTGTTGCTGTCTTTCTAAAAAAATTATTTCCCTTTTTCACTTCTGCACTGTGCCTTTATTCGACCACGACAAAATGATGCCGACTTTGAATTTTTTCTGCATTCTTTTATCTCTTCGCCAATGCCTTTGTGTTAAAGTACTTTTAATCTGAAGGATCTTGAATGGTGGGTTTAACTTTTGTTGACTAGGACTGTGATCTGCTCTTTTCCTTTCTTCTTTTTTTAATCTTGGTTGCCTTTTCCTTATTATATGTTTCTATATATATGTTTGGTCCCAATATGGACTTCATATCATTACTGCATGAACTTATAGCATACTGAATCATTGATCCTTCTGACTTGATAGAATGAAAGTTTGAAATTTTCGTTGAGAAATATTGAAACCATTAAATTTACCCTTCTCAAGTTTTTCACGGTGCCCAGTATCAACTTTCTCAGCTTTCATATTCTTCATACGGTTTCGGGAAAGGACGGATTCCTTTGAACTTTGCTTAAGAAGATGTAAAAATCTTCAATGAGAAGCCTAGCCTAAGCAGCTTGACTTTAATACACTAAAATATTAAGGGCCCACTACCCCTATATGCTTTAAGCTCATGGACATCATTTCAAACTCCATGATCTAACATGGTATGAAAGTAGCAAGTCTTGAATTGCAATCCAATGTTATTTTCTTTTCTTTTTTCTTTTCTCAATTTTGTATCGGGCGTTTTTGCTAACGTTTGTCTCATTTGAGGGGAGTACTGATTATATATGAAAAAGAAAAATTGAATATATCCTAACCTATCTTACTTTAGTTGTGGAAGAACAACTTTCAATTTTACTTTATCTGATTAGTGGTACTTAACTTTATTGGACTATAGTGAATAATGGATTTAATATATGATGAGAGGAAAAACAACGAAATGGTTGGTTGTGTCGATGTATTTGCATCTATGGTAGGCAACGTACAATGGATGCAATGGGGAATATCGTTGTGAAGTGATGCTGCAAGCCTGCAACAACATCTCTTGGGGAAGATAATGAATAACATGATTCTTATCCAAAAAAAGATAATGAATAACATGATAACTGAATCTTAAGGAAAATCTATAGGCTATAGCTCACAACCATTCCCAATATTCCCGATCTTACTCTGTTTGCGGTTGTGTATACGGCCTCAGGTTTACCCCCCGAGGAGGGTAACTCAAGGTTAAAGTTTTGTAGCCTTGGGATTGTTAGAGGATGGGAGAAGGGAGGAAAATGGAACAGATGGGAAACGGGAGGAAAAAGGAACAAGGAGCTGATGTGTAGCAAGACATCACGGGTTTGGCTTCTGTAACCTAGGGTTGAGAAGCCGACCAAATGTTTTGTTACACGGCTTGCTTATCCTAAGCAACAACTTTGCTAATTTCATAGGCGACACAAACCAATATTCTATAAATCTTGAATTATGAAACCCAATAGTTAGACAAATTCAGGTTTCCATGACGTATTCCTTTATACAATCCAAACATAGATTATACAAACTCAAACTTTTCAATAAACCCAACCTCATTAAACCGGGAGCTAAACATCCCCGGAAAATTAATGGTATTGGTGATGACCAAAATCAAGTCCTGTTCTTCATGTTGACTCTGACTGCACTCGTTTTAGAACTGTTATTAAATTAGAGGAGTGAAAGGAAAGAAAAGTAAATTGACATCTGAAAGGGGGCCAGCTTACTCAAATAAGAATCCATTGATTTGACCTCCAAATTGTTTAGTTGGGCCTGAAACCAAAATAATGGATGCCAAGGCTTATGGCTGGGAATAAATGCGGTGTCAGATAATCATTTCTTTGGTCATGTAGGTCATAGCTTGGGCTGTTGGTGGGTTTCCTGTGGCTGAACATCCCCCTAGTATTTGGTTTTGAACATGAATAGCTATATGTATTATCATTTTATATTGCTGAAGTTCAATCGTTTTGTCTATTCCATTCAAAGACTGAGATTCGCATGAACATTGATGGAAAGATGTTGTATACCTTTAGTTGAAAAAATTTTTCAAAATTGAAGTCTTCTGTGTCATAACAGTAATCTGAAGTTGAAGGATATATTCATAGGAGACAGTTACAGACTAATTTTTTACAGTTAATATGTTTCATAATCATGAACATTAGTATTTTGACTATTGTGAATGAACTGTCAACCCCTTCATTCTCAATCTTTTTGTTATTTTCAAGTTAAATTACCATTTTAGTTCTTATAGGCTTAATTTCAATTTGGTCCCTGTAGTCGTAAAAACTTCATAATCATCTTTTTCCTTATGTTAATAGTTTTCTTTACCTGTCATGTTTTTGTTGGACGTGACAGAAAGAGGTGATGAAATTGCCTTTAGTAAGGTTAAACAGGTGGATCTTTGAGTAAAATCCACTATTCTCTCTCCGCCCTGTCTAAAAAAACATGTCACATCCAGAAAAATGTTAACGTTCAGTAATAGCATAGGCGTTCTGGGAGATTTAACCAAACGATAGTGAGCACAACAAAACTTTTAAAACTAATAGAGTTCAAATTGAAACCAAGCGCAAATCACAAGGACTAAAATGGTAATTTAACCTTATTTTGATTACGAATCAACTATTCATGTGAACGACCCTATTGTTTCTCTTTACTTTCTGTGTAGTCTGTTCTTTCTTTTCCACCTGTCTTTTTCTTTTTCTTTTCTTTCTGAGATTGTGCTTTTATGATGAGTGTTGAGATAGATCATCCCTGGTTTTATTTATGGTAATATATTCTTCACATTCAGGTATGATTTCCCCTGGAGTCTAACGAAATTTATACCATTTTACGGTGGTGCGGAGTATCATGATTACCATCACTATGTTGGAGGACAAAGCCAAAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGTAGTTATTGGTCTTGTTTGTTATAATTTTACTTCCCACTTGTTCCAGTTAAATCATGCTCTTAAATTTTACTTATTTTTACTTCCTCAGGGGTATCGCTATCAAAAGAAGATCCTCAAGACGGTAAGTGCTTCATTTTCGTCCCCGTCCCCACCTCTGCCCCGAAATCTGATCGTATATGGTGTCATGAATTTGTATCTAGTTTCATGTTTTTCGATGGCCTGGCCGTTTTAAACTTGATTAAGGTTTAGGATGGCTAGTTTAGTTTAACTATTTTTTGCTCTTGCTTTCTCCTGTTTTTATTTGTTGGATCAAACCTGTTTCATCTGAAACAGTTGCCTCTTTTTGCTCTCAGCATCTGTTCTATTTGTCATTTTGCTTTCTTTTGGTTTTGGGGGGTAGAGATGGTGTAGAAAAAGAAAAAGGAAGGCGGATTTATAACTGTCATGTGCATTTTTCGTTGTTCTGGTCGATATATCGTCTACTTTGCGGTTGGCGACACGATATCGTGTGTTCTAATTATTTGTTGTTGGTGTGTAATCAGTTGAAGGAAGAAGGGAAGAGCAGTGAAGGGTCATACTACAGCACAGCCCAAGGTGTTAAATCGGACTAGACGACGGAGGGGACTCATGTTTTGAAGGATGCATTGTTCGTGGGCGTAGCAACGTTATGTAACCTCTGATTTTTGAAGTTAACTTAATCGTGATCGTATTTCCGACGAGGTAGTTAGGGACTGGAATGGGATGGAAATTAACTTGAGGTCTCAAAGAAGTTGTTTTTGTTAGCTTAATCTTCTTCAGGCTCAGAATTCAGATTCTCCAATGGCTTGTGTGTTTAACCTTATTGTGCAGACAAAACCCCTAATATAAATATCTATT

mRNA sequence

TTCACAACTTTTTAATCTAAAATAGATATCTTAACCTCGATTGATTTAGATTTATTACGAAAGAGAGAGTCGGGAATTGGTGTGTTACTAAGTTGAGTTGGGAGAGAGAAGGCACGTGAAAGATTTGGAAGAGAGAGAGTGGAGAGAGAAGGCATGTGAAAGATTTGGAAGGGAGTGACAGACGGCAATGGAGTGGACAGCATTCTCCTCCGCACATATATATAAACAAACATTGAATCCACAGAATTTCACGGCCTTCCTCTTGTTTTCTTCTCTTCTCTGTCCTTCAAAATTTCCCGCATAATTTTAACTTTCTGGGTTTCTCTCCGATTCCCGATTTCGATTCTCTTCTTCCATTCATATTTCATCCCTTTTGGCCGCTTCTTCCTCCGCCGGCGGCGTAAATTCCATCTGGGTTCTCCTCCGCCGCTCCCCAATGTTGCCGTTTCAGTCCCTCCACGAAGCCCAGTCGGCGCTTGGCCGGAATCTCACCTTCGCCGAGACCCTCTGGTTCAATTACTCCGCCGATAAGTCCGATTACTTCCTCTATTGCCACAACATTCTCTTCCTCTTCCTCATTTTCTCCTTTATTCCCCTCCCCCTCGTCTTCCTCGAGATTCTTCACGCAGCCGGAATTTATAAGTACAAAATTCAGCCAAAAGTCCGATTGCCCTTTTCCGAGATGTTCCGTTGCTACAGAGATGTCATGCGGATGTTCTTCCTCATCGTTGGCCCTCTCCAACTCATCTCCTTCCCTTGCATTAAGATGATTGGGATCAGGACAGGGTTGCCATTGCCTTCAGGATGGGAAATTCTTTCCCAATTACTGGTTTATTTCATGATCGAGGATTATACAAACTATTGGGTTCATAGATTCCTTCACTGCAAATGGGGGTACGAGAAAATCCATCGGGTTCACCATGAATACACAGCTCCTATAGGATTTGCAGCACCATATGCTCATTGGGCTGAGGTTTTGGTTCTTGGAATTCCCTCTTTTCTTGGTCCAGCCATGGTTCCTGGTCATATGATCACCTTCTGGTTGTGGATTGCTTTAAGGCAGATTGAGGCCATTGAAACGCATAGTGGGTATGATTTCCCCTGGAGTCTAACGAAATTTATACCATTTTACGGTGGTGCGGAGTATCATGATTACCATCACTATGTTGGAGGACAAAGCCAAAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGGGTATCGCTATCAAAAGAAGATCCTCAAGACGTTGAAGGAAGAAGGGAAGAGCAGTGAAGGGTCATACTACAGCACAGCCCAAGGTGTTAAATCGGACTAGACGACGGAGGGGACTCATGTTTTGAAGGATGCATTGTTCGTGGGCGTAGCAACGTTATGTAACCTCTGATTTTTGAAGTTAACTTAATCGTGATCGTATTTCCGACGAGGTAGTTAGGGACTGGAATGGGATGGAAATTAACTTGAGGTCTCAAAGAAGTTGTTTTTGTTAGCTTAATCTTCTTCAGGCTCAGAATTCAGATTCTCCAATGGCTTGTGTGTTTAACCTTATTGTGCAGACAAAACCCCTAATATAAATATCTATT

Coding sequence (CDS)

ATGTTGCCGTTTCAGTCCCTCCACGAAGCCCAGTCGGCGCTTGGCCGGAATCTCACCTTCGCCGAGACCCTCTGGTTCAATTACTCCGCCGATAAGTCCGATTACTTCCTCTATTGCCACAACATTCTCTTCCTCTTCCTCATTTTCTCCTTTATTCCCCTCCCCCTCGTCTTCCTCGAGATTCTTCACGCAGCCGGAATTTATAAGTACAAAATTCAGCCAAAAGTCCGATTGCCCTTTTCCGAGATGTTCCGTTGCTACAGAGATGTCATGCGGATGTTCTTCCTCATCGTTGGCCCTCTCCAACTCATCTCCTTCCCTTGCATTAAGATGATTGGGATCAGGACAGGGTTGCCATTGCCTTCAGGATGGGAAATTCTTTCCCAATTACTGGTTTATTTCATGATCGAGGATTATACAAACTATTGGGTTCATAGATTCCTTCACTGCAAATGGGGGTACGAGAAAATCCATCGGGTTCACCATGAATACACAGCTCCTATAGGATTTGCAGCACCATATGCTCATTGGGCTGAGGTTTTGGTTCTTGGAATTCCCTCTTTTCTTGGTCCAGCCATGGTTCCTGGTCATATGATCACCTTCTGGTTGTGGATTGCTTTAAGGCAGATTGAGGCCATTGAAACGCATAGTGGGTATGATTTCCCCTGGAGTCTAACGAAATTTATACCATTTTACGGTGGTGCGGAGTATCATGATTACCATCACTATGTTGGAGGACAAAGCCAAAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGGGTATCGCTATCAAAAGAAGATCCTCAAGACGTTGAAGGAAGAAGGGAAGAGCAGTGAAGGGTCATACTACAGCACAGCCCAAGGTGTTAAATCGGACTAG

Protein sequence

MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLEILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPLPSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEVLVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSEGSYYSTAQGVKSD
Homology
BLAST of MC10g1192 vs. ExPASy Swiss-Prot
Match: Q1EC69 (Methylsterol monooxygenase 1-2 OS=Arabidopsis thaliana OX=3702 GN=SMO1-2 PE=2 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 4.1e-135
Identity = 220/288 (76.39%), Postives = 256/288 (88.89%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           M+P+ ++ EA  AL RNLT+ ETLWF+YSA KSDY+LYCHNILFLFLIFS +PLPLVF+E
Sbjct: 1   MIPYATIEEASIALSRNLTWLETLWFDYSATKSDYYLYCHNILFLFLIFSLVPLPLVFIE 60

Query: 61  ILHAAG--IYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGL 120
              +      +YKIQPKV+  FS MF+CY+DVM+MF L+VGPLQL+S+P I+MI IR+GL
Sbjct: 61  SSQSTSDLFNRYKIQPKVKNSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWA 180
           PLPS  EI++QL+VYF++EDYTNYWVHRF HCKWGYEK H +HHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPSCMEIVAQLVVYFLVEDYTNYWVHRFFHCKWGYEKFHHIHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYH 240
           EVL+LGIP+FLGPA+ PGHMITFWLWIALRQIEAIETHSGYDFPWSLTK+IPFYGGAEYH
Sbjct: 181 EVLLLGIPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSS 287
           DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYR+QKK+L+ +KE+ K S
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQMKEKSKKS 288

BLAST of MC10g1192 vs. ExPASy Swiss-Prot
Match: Q8L7W5 (Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana OX=3702 GN=SMO1-1 PE=1 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 1.3e-133
Identity = 224/302 (74.17%), Postives = 263/302 (87.09%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           M+P+ ++ EA  ALGRNLT  ETLWF+YSA KSDY+LYCHNILFLFL+FS +PLPLVF+E
Sbjct: 1   MIPYATVEEASIALGRNLTRLETLWFDYSATKSDYYLYCHNILFLFLVFSLVPLPLVFVE 60

Query: 61  ILH-AAGIY-KYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGL 120
           +   A+G++ +YKIQPKV    S+MF+CY+DVM MF L+VGPLQL+S+P I+MI IR+GL
Sbjct: 61  LARSASGLFNRYKIQPKVNYSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWA 180
           PLP+  E+LSQL+VYF+IEDYTNYWVHRF H KWGY+KIHRVHHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPTITEMLSQLVVYFLIEDYTNYWVHRFFHSKWGYDKIHRVHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYH 240
           EVL+LGIP+F+GPA+ PGHMITFWLWIALRQ+EAIETHSGYDFPWS TK+IPFYGGAEYH
Sbjct: 181 EVLLLGIPTFMGPAIAPGHMITFWLWIALRQMEAIETHSGYDFPWSPTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSEGSYYSTAQGVK 300
           DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYR+QKK+L+ +KE  K S         G+K
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKSN----KHNGGIK 298

BLAST of MC10g1192 vs. ExPASy Swiss-Prot
Match: Q9AV39 (Very-long-chain aldehyde decarbonylase GL1-10 OS=Oryza sativa subsp. japonica OX=39947 GN=GL1-10 PE=2 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 4.0e-122
Identity = 200/285 (70.18%), Postives = 241/285 (84.56%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           MLP+ +  EA++ALGR +T AE+LWF YSA   DY L+ HNILFLF++F+  PLP+  LE
Sbjct: 1   MLPYATAAEAEAALGRAMTAAESLWFRYSAGIPDYVLFWHNILFLFVVFTLAPLPVALLE 60

Query: 61  ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL 120
           +   A +  +K+QPKVRL   E FRCYRDVMR+FFL++GPLQL+S+P +KM+GI TGLPL
Sbjct: 61  LRAPAAVGPFKLQPKVRLSREEFFRCYRDVMRLFFLVIGPLQLVSYPTVKMVGIHTGLPL 120

Query: 121 PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PS  E+ +QLLVYF++EDY NYW+HR LH +WGYEKIHRVHHE+TAPIGFAAPYAHWAEV
Sbjct: 121 PSLGEMAAQLLVYFLVEDYLNYWIHRLLHGEWGYEKIHRVHHEFTAPIGFAAPYAHWAEV 180

Query: 181 LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY 240
           L+LGIPSF+GPA+ PGHMITFWLWI LRQ+EAIETHSG+DFP++LTK+IPFYGGAEYHDY
Sbjct: 181 LILGIPSFVGPALAPGHMITFWLWIVLRQMEAIETHSGFDFPFNLTKYIPFYGGAEYHDY 240

Query: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKS 286
           HHYVG QSQSNFASVFTYCDY+YGTDKGYRY K     +K  G++
Sbjct: 241 HHYVGRQSQSNFASVFTYCDYLYGTDKGYRYHKAYQAKMKALGQT 285

BLAST of MC10g1192 vs. ExPASy Swiss-Prot
Match: F4JLZ6 (Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana OX=3702 GN=SMO1-3 PE=2 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 9.8e-121
Identity = 194/285 (68.07%), Postives = 245/285 (85.96%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           M+P+ ++ +A  ALGRNLT+ ET+WF+YSA KS++ +YCH IL LFL+FS  P PLV +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL 120
                   ++KIQ KV+   S+MF+CY++VM++F L+VG LQ++S+P I+M+GIR+GLPL
Sbjct: 61  --WTGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLPL 120

Query: 121 PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PS  EI++QL+VYF+IEDYTNYW+HR++HCKWGYEKIHR+HHEYT+PIG+A+PYAHWAE+
Sbjct: 121 PSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAEI 180

Query: 181 LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY 240
           L+LGIP+FLGPA+ PGH++TFWLWI+LRQ EAIETHSGYDFPWS+TK IPFYGG EYHDY
Sbjct: 181 LILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHDY 240

Query: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKIL-KTLKEEGK 285
           HHYVGGQSQSNFASVFTYCDYIYGTDKGYR  KK+L   +KEE +
Sbjct: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLLHHQIKEEAE 283

BLAST of MC10g1192 vs. ExPASy Swiss-Prot
Match: Q8VWZ8 (Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana OX=3702 GN=SMO2-2 PE=2 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 4.3e-60
Identity = 119/266 (44.74%), Postives = 161/266 (60.53%), Query Frame = 0

Query: 19  TFAETLWFNYSADKSDYFLYCHNILFLF-LIFSFIPLPLVFLEILHAAGIYKYKIQPKVR 78
           +F E+ W       SD+ L C     L   +F    LP +FLE      + KYKIQ K  
Sbjct: 3   SFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLE--RQGFLSKYKIQTKNN 62

Query: 79  LPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPLPSGWEILSQLLVYFMIE 138
            P ++  +C   ++   F +  PL L S+P  + +G+R+  PLPS  E+ +Q+L YF+IE
Sbjct: 63  TPAAQ-GKCITRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIE 122

Query: 139 DYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEVLVLGIPSFLGPAMVPGH 198
           D+  YW HR LH KW Y+ +H VHHEY  P G  + YAH AE+L LG  + +GPA+   H
Sbjct: 123 DFVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPH 182

Query: 199 MITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDYHHYVGGQSQSNFASVFT 258
           +IT WLW+ LR +E +E H GY FPWSL+ F+P YGGA++HDYHH +      N++S F 
Sbjct: 183 LITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFV 242

Query: 259 YCDYIYGTDKGYRYQKKILKTLKEEG 284
           Y D+I+GTDKGYR     LKTLKE G
Sbjct: 243 YMDWIFGTDKGYRR----LKTLKENG 261

BLAST of MC10g1192 vs. NCBI nr
Match: XP_022152787.1 (methylsterol monooxygenase 1-1-like [Momordica charantia])

HSP 1 Score: 625 bits (1611), Expect = 3.78e-226
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE
Sbjct: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60

Query: 61  ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL 120
           ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL
Sbjct: 61  ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL 120

Query: 121 PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY 240
           LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY
Sbjct: 181 LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY 240

Query: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSEGSYYSTAQGVKSD 300
           HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSEGSYYSTAQGVKSD
Sbjct: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSEGSYYSTAQGVKSD 300

BLAST of MC10g1192 vs. NCBI nr
Match: XP_023552163.1 (methylsterol monooxygenase 1-2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 571 bits (1472), Expect = 5.87e-205
Identity = 268/300 (89.33%), Postives = 287/300 (95.67%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           MLPF SL  AQ ALGRNLTF ETLWFNYS DKSDYFL+CHNILFLFLIFSFIPLPL+FLE
Sbjct: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL 120
           ILHA+GI+KYKIQPKVRLPFSE+FRCY+DVMRMFFL+VGPLQL+SFP I+MIGIRTGLPL
Sbjct: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEI+SQL+VYFM+EDYTNYW+HRFLHCKWGYE IH+VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY 240
           L+LGIPSFLGPAMVPGHMITFWLWIALRQIEAI+THSGYDFPWSLTKFIPFYGGA++HDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSEGSYYSTAQGVKSD 300
           HHYVGGQS SNFASVFTYCDYIYGTDKGYRYQKKILK LKEE KSSEGSYYSTA+ VK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

BLAST of MC10g1192 vs. NCBI nr
Match: XP_022923513.1 (methylsterol monooxygenase 1-2 [Cucurbita moschata] >KAG6577591.1 Methylsterol monooxygenase 1-2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015639.1 Methylsterol monooxygenase 1-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 570 bits (1469), Expect = 1.68e-204
Identity = 267/300 (89.00%), Postives = 287/300 (95.67%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           MLPF SL  AQ ALGRNLTF ETLWFNYS DKSDYFL+CHNILFLFLIFSFIPLPL+FLE
Sbjct: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL 120
           ILHA+GI+KYKIQPKVRLPFSE+FRCY+DVMRMFFL+VGPLQL+SFP I+MIGIRTGLPL
Sbjct: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEI+SQL+VYFM+EDYTNYW+HRFLHCKWGYE IH+VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY 240
           L+LGIPSFLGPAMVPGHMITFWLWIALRQIEAI+THSGYDFPWSLTKFIPFYGG+++HDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGSDHHDY 240

Query: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSEGSYYSTAQGVKSD 300
           HHYVGGQS SNFASVFTYCDYIYGTDKGYRYQKKILK LKEE KSSEGSYYSTA+ VK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

BLAST of MC10g1192 vs. NCBI nr
Match: XP_022965134.1 (methylsterol monooxygenase 1-2 [Cucurbita maxima])

HSP 1 Score: 569 bits (1466), Expect = 4.82e-204
Identity = 266/300 (88.67%), Postives = 287/300 (95.67%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           MLPF S+  AQ ALGRNLTF ETLWFNYS DKSDYFL+CHNILFLFLIFSFIPLPL+FLE
Sbjct: 1   MLPFHSISAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL 120
           ILHA+GI+K+KIQPKVRLPFSE+FRCY+DVMRMFFL+VGPLQL+SFP I+MIGIRTGLPL
Sbjct: 61  ILHASGIHKFKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEI+SQL+VYFM+EDYTNYW+HRFLHCKWGYE IH+VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY 240
           L+LGIPSFLGPAMVPGHMITFWLWIALRQIEAI+THSGYDFPWSLTKFIPFYGGA++HDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSEGSYYSTAQGVKSD 300
           HHYVGGQS SNFASVFTYCDYIYGTDKGYRYQKKILK LKEE KSSEGSYYSTA+ VK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

BLAST of MC10g1192 vs. NCBI nr
Match: XP_038876881.1 (methylsterol monooxygenase 1-2-like [Benincasa hispida])

HSP 1 Score: 568 bits (1465), Expect = 6.85e-204
Identity = 266/300 (88.67%), Postives = 287/300 (95.67%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           MLPF SL  AQ+ALGRNLTF ETLWFNYS DKSDYFL+CHNILFLFLIFSFIPLPL+FLE
Sbjct: 1   MLPFHSLSAAQAALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL 120
           +LHA+GI+KYKIQPKVRLPFSE+FRCY+DVMRMFFL+VGPLQL+SFP I+MIGIRTGLPL
Sbjct: 61  LLHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEI+SQL+VYFM+EDYTNYW+HRFLHCKWGYEKIH VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIISQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY 240
           L+LGIPSFLGPAMVPGHMITFWLWIALRQIEAI+THSGYDFPWSLTKFIPFYGGA++HDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSEGSYYSTAQGVKSD 300
           HHYVGGQS SNFASVFTYCDYIYGTDKGYRYQKKILK LKEE K+ EG Y+STAQ VKSD
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKQLKEEVKNIEGPYHSTAQDVKSD 300

BLAST of MC10g1192 vs. ExPASy TrEMBL
Match: A0A6J1DH80 (methylsterol monooxygenase 1-1-like OS=Momordica charantia OX=3673 GN=LOC111020420 PE=3 SV=1)

HSP 1 Score: 625 bits (1611), Expect = 1.83e-226
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE
Sbjct: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60

Query: 61  ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL 120
           ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL
Sbjct: 61  ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL 120

Query: 121 PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY 240
           LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY
Sbjct: 181 LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY 240

Query: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSEGSYYSTAQGVKSD 300
           HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSEGSYYSTAQGVKSD
Sbjct: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSEGSYYSTAQGVKSD 300

BLAST of MC10g1192 vs. ExPASy TrEMBL
Match: A0A6J1E9U9 (methylsterol monooxygenase 1-2 OS=Cucurbita moschata OX=3662 GN=LOC111431186 PE=3 SV=1)

HSP 1 Score: 570 bits (1469), Expect = 8.14e-205
Identity = 267/300 (89.00%), Postives = 287/300 (95.67%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           MLPF SL  AQ ALGRNLTF ETLWFNYS DKSDYFL+CHNILFLFLIFSFIPLPL+FLE
Sbjct: 1   MLPFHSLSAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL 120
           ILHA+GI+KYKIQPKVRLPFSE+FRCY+DVMRMFFL+VGPLQL+SFP I+MIGIRTGLPL
Sbjct: 61  ILHASGIHKYKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEI+SQL+VYFM+EDYTNYW+HRFLHCKWGYE IH+VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY 240
           L+LGIPSFLGPAMVPGHMITFWLWIALRQIEAI+THSGYDFPWSLTKFIPFYGG+++HDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGSDHHDY 240

Query: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSEGSYYSTAQGVKSD 300
           HHYVGGQS SNFASVFTYCDYIYGTDKGYRYQKKILK LKEE KSSEGSYYSTA+ VK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

BLAST of MC10g1192 vs. ExPASy TrEMBL
Match: A0A6J1HQ57 (methylsterol monooxygenase 1-2 OS=Cucurbita maxima OX=3661 GN=LOC111465081 PE=3 SV=1)

HSP 1 Score: 569 bits (1466), Expect = 2.33e-204
Identity = 266/300 (88.67%), Postives = 287/300 (95.67%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           MLPF S+  AQ ALGRNLTF ETLWFNYS DKSDYFL+CHNILFLFLIFSFIPLPL+FLE
Sbjct: 1   MLPFHSISAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL 120
           ILHA+GI+K+KIQPKVRLPFSE+FRCY+DVMRMFFL+VGPLQL+SFP I+MIGIRTGLPL
Sbjct: 61  ILHASGIHKFKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120

Query: 121 PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSGWEI+SQL+VYFM+EDYTNYW+HRFLHCKWGYE IH+VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY 240
           L+LGIPSFLGPAMVPGHMITFWLWIALRQIEAI+THSGYDFPWSLTKFIPFYGGA++HDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSEGSYYSTAQGVKSD 300
           HHYVGGQS SNFASVFTYCDYIYGTDKGYRYQKKILK LKEE KSSEGSYYSTA+ VK D
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD 300

BLAST of MC10g1192 vs. ExPASy TrEMBL
Match: A0A0A0L5P8 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G036480 PE=3 SV=1)

HSP 1 Score: 564 bits (1453), Expect = 2.24e-202
Identity = 264/300 (88.00%), Postives = 287/300 (95.67%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           MLPF SL  AQ++LGRNLTF ETLWFNYS DKSDYFL+CHNILFLFLIFSFIPLPL+FLE
Sbjct: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSNDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL 120
           +LHA GI+KYKIQPKVRLPF+E+FRCY+DVMRMFFL+VGPLQL+SFP IKMIGIRTGLPL
Sbjct: 61  LLHATGIHKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120

Query: 121 PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSG+EI+SQL+VYFM+EDYTNYW+HRFLHCKWGYEKIH VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY 240
           L+LGIPSFLGPAMVPGHMITFWLWIALRQIEAI+THSGYDFPWSLTKFIPFYGGA++HDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSEGSYYSTAQGVKSD 300
           HHYVGGQS SNFASVFTYCDYIYGTDKGYRYQKKIL+ LKEE K+SE SYY+TAQ VKSD
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESYYNTAQNVKSD 300

BLAST of MC10g1192 vs. ExPASy TrEMBL
Match: A0A1S3BKU6 (methylsterol monooxygenase 1-2-like OS=Cucumis melo OX=3656 GN=LOC103490939 PE=3 SV=1)

HSP 1 Score: 559 bits (1440), Expect = 2.22e-200
Identity = 264/301 (87.71%), Postives = 287/301 (95.35%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           MLPF SL  AQ++LGRNLTF ETLWFNYS DKSDYFL+CHNILFLFLIFSFIPLPL+FLE
Sbjct: 1   MLPFHSLSAAQASLGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60

Query: 61  ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL 120
           +LHA+GI KYKIQPKVRLPF+E+FRCY+DVMRMFFL+VGPLQL+SFP IKMIGIRTGLPL
Sbjct: 61  LLHASGIRKYKIQPKVRLPFNEIFRCYKDVMRMFFLVVGPLQLVSFPSIKMIGIRTGLPL 120

Query: 121 PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PSG+EI+SQL+VYFM+EDYTNYW+HRFLHCKWGYEKIH VHHEYTAPIGFAAPYAHWAEV
Sbjct: 121 PSGFEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYEKIHCVHHEYTAPIGFAAPYAHWAEV 180

Query: 181 LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY 240
           L+LGIPSFLGPAMVPGHMITFWLWIALRQIEAI+THSGYDFPWSLTKFIPFYGGA++HDY
Sbjct: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240

Query: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSE-GSYYSTAQGVKS 300
           HHYVGGQS SNFASVFTYCDYIYGTDKGYRYQKKIL+ LKEE K+SE  SYY+TAQ VKS
Sbjct: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILQKLKEEVKNSEESSYYNTAQNVKS 300

BLAST of MC10g1192 vs. TAIR 10
Match: AT4G22756.1 (sterol C4-methyl oxidase 1-2 )

HSP 1 Score: 482.3 bits (1240), Expect = 2.9e-136
Identity = 220/288 (76.39%), Postives = 256/288 (88.89%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           M+P+ ++ EA  AL RNLT+ ETLWF+YSA KSDY+LYCHNILFLFLIFS +PLPLVF+E
Sbjct: 1   MIPYATIEEASIALSRNLTWLETLWFDYSATKSDYYLYCHNILFLFLIFSLVPLPLVFIE 60

Query: 61  ILHAAG--IYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGL 120
              +      +YKIQPKV+  FS MF+CY+DVM+MF L+VGPLQL+S+P I+MI IR+GL
Sbjct: 61  SSQSTSDLFNRYKIQPKVKNSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWA 180
           PLPS  EI++QL+VYF++EDYTNYWVHRF HCKWGYEK H +HHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPSCMEIVAQLVVYFLVEDYTNYWVHRFFHCKWGYEKFHHIHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYH 240
           EVL+LGIP+FLGPA+ PGHMITFWLWIALRQIEAIETHSGYDFPWSLTK+IPFYGGAEYH
Sbjct: 181 EVLLLGIPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSS 287
           DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYR+QKK+L+ +KE+ K S
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQMKEKSKKS 288

BLAST of MC10g1192 vs. TAIR 10
Match: AT4G12110.1 (sterol-4alpha-methyl oxidase 1-1 )

HSP 1 Score: 477.2 bits (1227), Expect = 9.4e-135
Identity = 224/302 (74.17%), Postives = 263/302 (87.09%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           M+P+ ++ EA  ALGRNLT  ETLWF+YSA KSDY+LYCHNILFLFL+FS +PLPLVF+E
Sbjct: 1   MIPYATVEEASIALGRNLTRLETLWFDYSATKSDYYLYCHNILFLFLVFSLVPLPLVFVE 60

Query: 61  ILH-AAGIY-KYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGL 120
           +   A+G++ +YKIQPKV    S+MF+CY+DVM MF L+VGPLQL+S+P I+MI IR+GL
Sbjct: 61  LARSASGLFNRYKIQPKVNYSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWA 180
           PLP+  E+LSQL+VYF+IEDYTNYWVHRF H KWGY+KIHRVHHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPTITEMLSQLVVYFLIEDYTNYWVHRFFHSKWGYDKIHRVHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYH 240
           EVL+LGIP+F+GPA+ PGHMITFWLWIALRQ+EAIETHSGYDFPWS TK+IPFYGGAEYH
Sbjct: 181 EVLLLGIPTFMGPAIAPGHMITFWLWIALRQMEAIETHSGYDFPWSPTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKILKTLKEEGKSSEGSYYSTAQGVK 300
           DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYR+QKK+L+ +KE  K S         G+K
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKSN----KHNGGIK 298

BLAST of MC10g1192 vs. TAIR 10
Match: AT4G22753.1 (sterol 4-alpha methyl oxidase 1-3 )

HSP 1 Score: 434.5 bits (1116), Expect = 7.0e-122
Identity = 194/285 (68.07%), Postives = 245/285 (85.96%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           M+P+ ++ +A  ALGRNLT+ ET+WF+YSA KS++ +YCH IL LFL+FS  P PLV +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL 120
                   ++KIQ KV+   S+MF+CY++VM++F L+VG LQ++S+P I+M+GIR+GLPL
Sbjct: 61  --WTGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLPL 120

Query: 121 PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PS  EI++QL+VYF+IEDYTNYW+HR++HCKWGYEKIHR+HHEYT+PIG+A+PYAHWAE+
Sbjct: 121 PSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAEI 180

Query: 181 LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY 240
           L+LGIP+FLGPA+ PGH++TFWLWI+LRQ EAIETHSGYDFPWS+TK IPFYGG EYHDY
Sbjct: 181 LILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHDY 240

Query: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKIL-KTLKEEGK 285
           HHYVGGQSQSNFASVFTYCDYIYGTDKGYR  KK+L   +KEE +
Sbjct: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLLHHQIKEEAE 283

BLAST of MC10g1192 vs. TAIR 10
Match: AT4G22753.2 (sterol 4-alpha methyl oxidase 1-3 )

HSP 1 Score: 434.5 bits (1116), Expect = 7.0e-122
Identity = 194/285 (68.07%), Postives = 245/285 (85.96%), Query Frame = 0

Query: 1   MLPFQSLHEAQSALGRNLTFAETLWFNYSADKSDYFLYCHNILFLFLIFSFIPLPLVFLE 60
           M+P+ ++ +A  ALGRNLT+ ET+WF+YSA KS++ +YCH IL LFL+FS  P PLV +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  ILHAAGIYKYKIQPKVRLPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPL 120
                   ++KIQ KV+   S+MF+CY++VM++F L+VG LQ++S+P I+M+GIR+GLPL
Sbjct: 61  --WTGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLPL 120

Query: 121 PSGWEILSQLLVYFMIEDYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEV 180
           PS  EI++QL+VYF+IEDYTNYW+HR++HCKWGYEKIHR+HHEYT+PIG+A+PYAHWAE+
Sbjct: 121 PSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAEI 180

Query: 181 LVLGIPSFLGPAMVPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDY 240
           L+LGIP+FLGPA+ PGH++TFWLWI+LRQ EAIETHSGYDFPWS+TK IPFYGG EYHDY
Sbjct: 181 LILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHDY 240

Query: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRYQKKIL-KTLKEEGK 285
           HHYVGGQSQSNFASVFTYCDYIYGTDKGYR  KK+L   +KEE +
Sbjct: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLLHHQIKEEAE 283

BLAST of MC10g1192 vs. TAIR 10
Match: AT1G07420.1 (sterol 4-alpha-methyl-oxidase 2-1 )

HSP 1 Score: 233.0 bits (593), Expect = 3.1e-61
Identity = 119/266 (44.74%), Postives = 161/266 (60.53%), Query Frame = 0

Query: 19  TFAETLWFNYSADKSDYFLYCHNILFLF-LIFSFIPLPLVFLEILHAAGIYKYKIQPKVR 78
           +F E+ W       SD+ L C     L   +F    LP +FLE      + KYKIQ K  
Sbjct: 3   SFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLE--RQGFLSKYKIQTKNN 62

Query: 79  LPFSEMFRCYRDVMRMFFLIVGPLQLISFPCIKMIGIRTGLPLPSGWEILSQLLVYFMIE 138
            P ++  +C   ++   F +  PL L S+P  + +G+R+  PLPS  E+ +Q+L YF+IE
Sbjct: 63  TPAAQ-GKCITRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIE 122

Query: 139 DYTNYWVHRFLHCKWGYEKIHRVHHEYTAPIGFAAPYAHWAEVLVLGIPSFLGPAMVPGH 198
           D+  YW HR LH KW Y+ +H VHHEY  P G  + YAH AE+L LG  + +GPA+   H
Sbjct: 123 DFVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPH 182

Query: 199 MITFWLWIALRQIEAIETHSGYDFPWSLTKFIPFYGGAEYHDYHHYVGGQSQSNFASVFT 258
           +IT WLW+ LR +E +E H GY FPWSL+ F+P YGGA++HDYHH +      N++S F 
Sbjct: 183 LITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFV 242

Query: 259 YCDYIYGTDKGYRYQKKILKTLKEEG 284
           Y D+I+GTDKGYR     LKTLKE G
Sbjct: 243 YMDWIFGTDKGYRR----LKTLKENG 261

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q1EC694.1e-13576.39Methylsterol monooxygenase 1-2 OS=Arabidopsis thaliana OX=3702 GN=SMO1-2 PE=2 SV... [more]
Q8L7W51.3e-13374.17Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana OX=3702 GN=SMO1-1 PE=1 SV... [more]
Q9AV394.0e-12270.18Very-long-chain aldehyde decarbonylase GL1-10 OS=Oryza sativa subsp. japonica OX... [more]
F4JLZ69.8e-12168.07Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana OX=3702 GN=SMO1-3 PE=2 SV... [more]
Q8VWZ84.3e-6044.74Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana OX=3702 GN=SMO2-2 PE=2 SV... [more]
Match NameE-valueIdentityDescription
XP_022152787.13.78e-226100.00methylsterol monooxygenase 1-1-like [Momordica charantia][more]
XP_023552163.15.87e-20589.33methylsterol monooxygenase 1-2 [Cucurbita pepo subsp. pepo][more]
XP_022923513.11.68e-20489.00methylsterol monooxygenase 1-2 [Cucurbita moschata] >KAG6577591.1 Methylsterol m... [more]
XP_022965134.14.82e-20488.67methylsterol monooxygenase 1-2 [Cucurbita maxima][more]
XP_038876881.16.85e-20488.67methylsterol monooxygenase 1-2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1DH801.83e-226100.00methylsterol monooxygenase 1-1-like OS=Momordica charantia OX=3673 GN=LOC1110204... [more]
A0A6J1E9U98.14e-20589.00methylsterol monooxygenase 1-2 OS=Cucurbita moschata OX=3662 GN=LOC111431186 PE=... [more]
A0A6J1HQ572.33e-20488.67methylsterol monooxygenase 1-2 OS=Cucurbita maxima OX=3661 GN=LOC111465081 PE=3 ... [more]
A0A0A0L5P82.24e-20288.00Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C... [more]
A0A1S3BKU62.22e-20087.71methylsterol monooxygenase 1-2-like OS=Cucumis melo OX=3656 GN=LOC103490939 PE=3... [more]
Match NameE-valueIdentityDescription
AT4G22756.12.9e-13676.39sterol C4-methyl oxidase 1-2 [more]
AT4G12110.19.4e-13574.17sterol-4alpha-methyl oxidase 1-1 [more]
AT4G22753.17.0e-12268.07sterol 4-alpha methyl oxidase 1-3 [more]
AT4G22753.27.0e-12268.07sterol 4-alpha methyl oxidase 1-3 [more]
AT1G07420.13.1e-6144.74sterol 4-alpha-methyl-oxidase 2-1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 131..265
e-value: 6.8E-31
score: 107.4
NoneNo IPR availablePANTHERPTHR11863:SF201STEROL-4ALPHA-METHYL OXIDASE 1-1-RELATEDcoord: 1..300
NoneNo IPR availablePANTHERPTHR11863STEROL DESATURASEcoord: 1..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g1192.1MC10g1192.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016126 sterol biosynthetic process
biological_process GO:0008610 lipid biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0000254 C-4 methylsterol oxidase activity
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity