MC10g1010 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g1010
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	21.7 kDa class VI heat shock protein
Location	MC10: 9397517 .. 9400785 (-)
RNA-Seq Expression	MC10g1010
Synteny	MC10g1010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCACTTCAAAACAACTAGAAGTTCACAGAGAAGACCATACTCCATCAAAATGGAGTGTTTCTTTGGGAGAAGAAGCTTTCAGAAGATTTCTAGGGCAAGCTAATCCGGTTGTGCAAAAGGTATTCGGCGATGGCTCGTTGTTCAGTCCATTGCTGTTCGGAAAGTTCTTCGATCCAGCTGATGCTTTTCCGCTGTGGGAGTTTGAGTCGGATTTGTTATTGTCCAACCTCCGCAACTCAGGAAAATCAACGATCGATTGGTTTCAGATTGATCAAGAATATGTTCTCCAGGCAGAATTACCAGGTACTTTTCTTTGCCACTTGAGAGATTATTTTAGAAGAGTATGTTTTATGTATAATTAGAAGAGTTTTTGGACTGACTTTGGATAATTAAAAATAAAATCATTGAAATTATAAAATAAAAATGAATAAATGAATGAAATAAGTGAAAGAAAATAGGTATTGAGAGACGTTAGTGAAAAAAAAAATTGCATAAAAAGATCACTAACTATAGATGAATCAAGATTAGTGAAAGAAAATGTCATTTGAAGAGAGTGACAATAAAAAGAGATCTATAAGAGAGAAACTAAGAAGAGAGTGTGTGTAGGAGGGATTGATGAGAAAAAACATGTGCAATAAGAAGTGGCAAAATATATCAACTAAATAAAATAAGAGTGTGGTTTAGGAAAAGAAAAAATCAATTTAAATTTTATTCATCTAGCTTACTCCCACCTAAAAAAAATCTTTAAGTGTAATTAGAAACTTAACAAAGATTATTTAAAAGTATTTTAAAAAGTAAAATAACAAACGAGGAATAAACAAAGTTTTAAATTTCGATAGTTAATTTCGAGATTTCAATTTCATGAAAATGTCAATAAAAACATCATAAAATGTAGATATGGATAAGTATTTCTAAAAGAGTTGTAAAAATAAAAAATTCGTAAAAACTATTTAAATCAACAAATAACCATTTCATCATTTCTAAATCAGTTAAAAATAATTAATATTAATATCATGTCTATATTGATGAGATTTTGTTAATACTTTTCATATTTTGTCCATTTTTTTTGTAATATTATAGTTATGTCGATTTATCATACATGAAAATATCGATGGAAATGTCGACAGAAATTTAATACCACGGGTATAAATAGTTTTTTATAAAATGTAATTTTTAAAGAAAAAACCAAAAAGTAAAATTGTTGGGCTTAGTTTGCAAAAATTAGTTAAAGATCTCAAACATATCTTTTTTTTTTTTTTTTGGTATGAGATAGAAATAGTAAGAGGAGTCAAATTTGTTTTACATTAAAAAAGAACAAATAAATATCGAAGGAATCTGATTCACAGCACGGGCATTTCCCACATCGAAAGAACCACAACTCGGTAAGCTTTTTAAATTGGAGGAGAATCAATTTTGAAAGCAACTTCGGGGGTGGCGGGAAGGGAACTGCACGCGCTTACCCAAACCCTAAACCTGTTTAAAATTTGGGGTATTTTGCCAGCGTTTCCCCCGTGTGGACCTCAACAAAGAAACGCTGATGCACTGCGCGTGATTCAGTTTCCCGATTGCTTTACCGAACGCCCATGAGAGGATTGTAGGTGGTGCTGCCTGCCCCGTGCGTAGGGGTTGTTTGGCGGTCACTCTCGATCTCCCTCCTCGCCTCTCAACCACCCCATTTTTGGATCTTATAAGAGTCATTCCTATTTGATTAATTTAGCTTCTCTTTTTCCCTTTCTTATGAATGTTGCCAACACCTGTCATCTCTCTCTATATCGTCACCCTCACAGACAAACTTTCATTTCAAATCTACAAGGTCCGATGAGGAATGGTACTTGTTTCAAATCTAAAATATTCGATGAAGAGCGTTTAAAACGGGGTGTTCCCCTTTGAATATGGAATAAATAAGTACCCATCATTTCTCTTTTATGAGAGAAACCCTGATTCCATATCTAGAAGCGATTCAATTAGGAATATTACTCGTTCTAAATCTAGAAGATTCAGTGAAGAGTGTTTGAAACGAGGGTGTTCCTCATTGAATTTGGGATGAGAAGCATCCCTTATATCTGATTTGACGAGGAACAAGCATCCTTTGACGATACACCAATAATAACGATAGCATAGCAAAGAGGGGGTGACGAGCCAGGAGAGAAACGATTGGCAGTGTGGTAGGAGAAAGAGAGAGGAAGAGAAGAAGAAAAGAGAAAGAAAGTCCTGTCACCACAAATATATTATTAAATTAATATATTTTGTCAAATCAATAGGTACAATCAACAATTTAACCGCTACGTCATCTTAACCTTAATCAACAAACGGTTAGGGGAGCTCAATAATAACTACTTAGAATCATTTCAAATCTCAATGACCTAGGTCTTTTTGAAACTTCAATAACCACGTTCCTACTTCAAGTCTCAAGAGCCCAAAACTATTGTTATTACTCTTACTAATCTCTCACTTAATTTGTTTGGTTTGTATTTATAATTATTTTCATTTTTAGTGATTTATCCATTGGAGAAATACTTGTTACGTAAATATTAATCTTTCAAAAATAGTTCTAGCTGGATCAAAAGCATTTTTGAAATACCAGAATTAAACAATTGAGACTTTTATATTAAGCATTCTTTAATAATTTTAACAGCAGTACTGTTTCTAGTGAAAGCACCCTCCACCCAAAATCTTTTAATTTTTTTTAAGCATTTTAAGTATAGATTTTTGTAATGGATGATAAATAATAGTCAATACAGATATATATTTTTCGTAGGAAGTGCTTTTTAACTTCTATCATAGTTAAAAGCATTCTCAAACATTTATTTTGCAGAGAGTGTTGAAGTTGAAAGGATGATAAGAGAGAATAGATTTTAATGGTGAATTATGTATCAACTACATTTAAAAAGACATTCCCAAGGCCTTCAATTCTGTTGCTACTTATGGTACAAAACTTGGGTGATATGTTTCATTGACACAAAGCAAAAATTTGACAAAAACAGAAGCTGTGAGAAACACAATGCAGATCTTCATAGAGAATGGGAAGGTCTTAGAGATAAGTGGTCAGTTGAAGCAGCAGCAGCAGAGGGAAAGCAAGACGATCATCGACTGGCGAAGTGTCAACTGGTGGGAGCATGGATATGTTCGGCGGCTCGAGTTGCCCGACGATGCTGATTGGCGGAGAATGGAGGCGCGTGTCAAAAACGACATGGTTATCGAGGTAAAAATTCCCAAGTTGGAAGCCAATCAAAGTGCTGAATCAAAGAACAAAGATTCTGAAGAT

mRNA sequence

ATGGCCACTTCAAAACAACTAGAAGTTCACAGAGAAGACCATACTCCATCAAAATGGAGTGTTTCTTTGGGAGAAGAAGCTTTCAGAAGATTTCTAGGGCAAGCTAATCCGGTTGTGCAAAAGGTATTCGGCGATGGCTCGTTGTTCAGTCCATTGCTGTTCGGAAAGTTCTTCGATCCAGCTGATGCTTTTCCGCTGTGGGAGTTTGAGTCGGATTTGTTATTGTCCAACCTCCGCAACTCAGGAAAATCAACGATCGATTGGTTTCAGATTGATCAAGAATATGTTCTCCAGGCAGAATTACCAGAAGCTGTGAGAAACACAATGCAGATCTTCATAGAGAATGGGAAGGTCTTAGAGATAAGTGGTCAGTTGAAGCAGCAGCAGCAGAGGGAAAGCAAGACGATCATCGACTGGCGAAGTGTCAACTGGTGGGAGCATGGATATGTTCGGCGGCTCGAGTTGCCCGACGATGCTGATTGGCGGAGAATGGAGGCGCGTGTCAAAAACGACATGGTTATCGAGGTAAAAATTCCCAAGTTGGAAGCCAATCAAAGTGCTGAATCAAAGAACAAAGATTCTGAAGAT

Coding sequence (CDS)

ATGGCCACTTCAAAACAACTAGAAGTTCACAGAGAAGACCATACTCCATCAAAATGGAGTGTTTCTTTGGGAGAAGAAGCTTTCAGAAGATTTCTAGGGCAAGCTAATCCGGTTGTGCAAAAGGTATTCGGCGATGGCTCGTTGTTCAGTCCATTGCTGTTCGGAAAGTTCTTCGATCCAGCTGATGCTTTTCCGCTGTGGGAGTTTGAGTCGGATTTGTTATTGTCCAACCTCCGCAACTCAGGAAAATCAACGATCGATTGGTTTCAGATTGATCAAGAATATGTTCTCCAGGCAGAATTACCAGAAGCTGTGAGAAACACAATGCAGATCTTCATAGAGAATGGGAAGGTCTTAGAGATAAGTGGTCAGTTGAAGCAGCAGCAGCAGAGGGAAAGCAAGACGATCATCGACTGGCGAAGTGTCAACTGGTGGGAGCATGGATATGTTCGGCGGCTCGAGTTGCCCGACGATGCTGATTGGCGGAGAATGGAGGCGCGTGTCAAAAACGACATGGTTATCGAGGTAAAAATTCCCAAGTTGGAAGCCAATCAAAGTGCTGAATCAAAGAACAAAGATTCTGAAGAT

Protein sequence

MATSKQLEVHREDHTPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFFDPADAFPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIENGKVLEISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKNDMVIEVKIPKLEANQSAESKNKDSED

Homology

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BLAST of MC10g1010 vs. ExPASy Swiss-Prot
Match: Q9FIT9 (21.7 kDa class VI heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP21.7 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 8.3e-52
Identity = 99/193 (51.30%), Postives = 138/193 (71.50%), Query Frame = 0


Query: 4   SKQLEVHREDHTPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFFDPADA 63
           S +LE+H +D TP KWSV LG++ FRRFL       + VFG+GSLFSP LFGK+FDP+DA
Sbjct: 7   SLKLEIHTDDKTPGKWSVPLGDDVFRRFLSGGGGSEKAVFGEGSLFSPFLFGKYFDPSDA 66

Query: 64  FPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIE-NGKVLEIS 123
           FPLWEFE+++LL++LR+ G+  +DW Q DQ YVL++++P   +N +Q++++ NG+V+EIS
Sbjct: 67  FPLWEFEAEVLLASLRSLGQCRVDWSQTDQAYVLKSDIPVVGKNNVQVYVDINGRVMEIS 126

Query: 124 GQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKND---MVIEVKIP 183
           GQ      +++ T  DWRS  WWEHGYVRRLELP DAD +  EA + N+     +E++IP
Sbjct: 127 GQ--WNSNKKAATNSDWRSGRWWEHGYVRRLELPSDADAKYSEAFLSNNDDYSFLEIRIP 186

Query: 184 KLEANQSAESKNK 193
           K+       SKNK
Sbjct: 187 KI------NSKNK 191

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BLAST of MC10g1010 vs. ExPASy Swiss-Prot
Match: Q6AUW3 (22.3 kDa class VI heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP22.3 PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 5.2e-38
Identity = 79/185 (42.70%), Postives = 119/185 (64.32%), Query Frame = 0


Query: 13  DHTPSKWSVSLGEEAFRRFL-------GQANPVVQKVFGDGSLFSPLLFGKFFDPADAFP 72
           D    +W +SL E  F  FL              + VFG+GSLFSP LFGKFFDPADAFP
Sbjct: 15  DGAAPRWRMSLLENTFSSFLQSIGGGVAADGAAARAVFGEGSLFSPFLFGKFFDPADAFP 74

Query: 73  LWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIENG-KVLEISGQ 132
           LWEFE ++LL+ LR   ++T+DW + D EY L+A++P   +  +++  ++  +V+++SG 
Sbjct: 75  LWEFEPEVLLAALRRGARTTVDWAETDSEYYLRADIPGGRKCDVEVSGDDAMRVVDVSGL 134

Query: 133 LKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKN-DMVIEVKIPKL-E 188
            +           DWR+  WWEHG+VRR+ELP+DADWR++EA   + + ++E+K+PK  +
Sbjct: 135 WRAAPPPPPPDGRDWRAGRWWEHGFVRRVELPEDADWRKVEAFFDDGEGLLEIKVPKSGD 194

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BLAST of MC10g1010 vs. ExPASy Swiss-Prot
Match: P27396 (17.8 kDa class I heat shock protein OS=Daucus carota OX=4039 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.5e-08
Identity = 43/145 (29.66%), Postives = 75/145 (51.72%), Query Frame = 0


Query: 51  PLLFG----KFFDPADAFPLWEFESD--LLLSNLRNSGKST-------IDWFQIDQEYVL 110
           P  FG      FDP  +  +W+   D  L+ S+    GK T       IDW +  Q +V 
Sbjct: 5   PSFFGGRRSNVFDPF-SLDVWDPFKDFPLVTSSASEFGKETAAFVNTHIDWKETPQAHVF 64

Query: 111 QAELPEAVRNTMQIFIENGKVLEISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPD 170
           +A+LP   +  +++ +E GKVL+ISG+  ++++ ++     W  V      ++RR  LP+
Sbjct: 65  KADLPGLKKEEVKVELEEGKVLQISGERNKEKEEKND---KWHRVERSSGKFLRRFRLPE 124

Query: 171 DADWRRMEARVKNDMVIEVKIPKLE 183
           +A    ++A + N  V+ V +PK+E
Sbjct: 125 NAKVDEVKAAMANG-VVTVTVPKVE 144

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BLAST of MC10g1010 vs. ExPASy Swiss-Prot
Match: P27397 (18.0 kDa class I heat shock protein OS=Daucus carota OX=4039 PE=3 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 9.6e-08
Identity = 30/97 (30.93%), Postives = 60/97 (61.86%), Query Frame = 0


Query: 86  IDWFQIDQEYVLQAELPEAVRNTMQIFIENGKVLEISGQLKQQQQRESKTIIDWRSVNWW 145
           IDW +  Q +V +A+LP   +  +++ +E GKVL+ISG+  ++++ ++     W  V + 
Sbjct: 54  IDWKETPQAHVFKADLPGLKKEEVKVEVEEGKVLQISGERNKEKEEKNN---KWHRVEFS 113

Query: 146 EHGYVRRLELPDDADWRRMEARVKNDMVIEVKIPKLE 183
              ++RR  LP++A+   ++A ++N  V+ V +PK+E
Sbjct: 114 SGKFLRRFRLPENANVDEVKAGMENG-VLTVTVPKVE 146

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BLAST of MC10g1010 vs. ExPASy Swiss-Prot
Match: P31673 (17.4 kDa class I heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP17.4 PE=2 SV=2)

HSP 1 Score: 58.2 bits (139), Expect = 1.2e-07
Identity = 36/129 (27.91%), Postives = 69/129 (53.49%), Query Frame = 0


Query: 58  FDPADAFPLWEFESDLL---LSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIE 117
           +DP D FP       L     S+      + IDW +  + +V +A++P   +  +++ +E
Sbjct: 18  WDPFDGFPFGSGSGSLFPRANSDAAAFAGARIDWKETPEAHVFKADVPGLKKEEVKVEVE 77

Query: 118 NGKVLEISGQ-LKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKNDMV 177
           +G VL+ISG+ +K+Q+++  K    W  V      ++RR  LP++    +++A ++N  V
Sbjct: 78  DGNVLQISGERIKEQEEKTDK----WHRVERSSGKFLRRFRLPENTKPEQIKASMENG-V 137

Query: 178 IEVKIPKLE 183
           + V +PK E
Sbjct: 138 LTVTVPKEE 141

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BLAST of MC10g1010 vs. NCBI nr
Match: XP_022154944.1 (21.7 kDa class VI heat shock protein [Momordica charantia])

HSP 1 Score: 395 bits (1015), Expect = 9.47e-139
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 0


Query: 1   MATSKQLEVHREDHTPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFFDP 60
           MATSKQLEVHREDHTPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFFDP
Sbjct: 1   MATSKQLEVHREDHTPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFFDP 60

Query: 61  ADAFPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIENGKVLE 120
           ADAFPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIENGKVLE
Sbjct: 61  ADAFPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIENGKVLE 120

Query: 121 ISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKNDMVIEVKIPK 180
           ISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKNDMVIEVKIPK
Sbjct: 121 ISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKNDMVIEVKIPK 180

Query: 181 LEANQSAESKNKDSED 196
           LEANQSAESKNKDSED
Sbjct: 181 LEANQSAESKNKDSED 196

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BLAST of MC10g1010 vs. NCBI nr
Match: XP_004152915.1 (21.7 kDa class VI heat shock protein isoform X1 [Cucumis sativus] >KGN56157.1 hypothetical protein Csa_011572 [Cucumis sativus])

HSP 1 Score: 317 bits (813), Expect = 6.50e-108
Identity = 161/198 (81.31%), Postives = 177/198 (89.39%), Query Frame = 0


Query: 1   MATSKQLEVHREDH-TPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFFD 60
           MATSKQLEVH+EDH TPSKWSVSLGEE FRRFLGQAN  +QKVFGDGSLFSPLLFGKFFD
Sbjct: 1   MATSKQLEVHKEDHPTPSKWSVSLGEEVFRRFLGQANSAIQKVFGDGSLFSPLLFGKFFD 60

Query: 61  PADAFPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIE-NGKV 120
           PADAFPLWEFESDLLLSNLR SGKS+IDW Q DQEYVLQAEL EA RN +QI IE +GKV
Sbjct: 61  PADAFPLWEFESDLLLSNLRISGKSSIDWSQTDQEYVLQAELLEAWRNALQISIEEDGKV 120

Query: 121 LEISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKNDMVIEVKI 180
           LEISGQLK+Q QRE KT +DWR V+WWEHGYVRRLELP+DAD  RMEAR+KND+V+E+KI
Sbjct: 121 LEISGQLKEQ-QREGKTTVDWRRVHWWEHGYVRRLELPEDADSSRMEARIKNDLVLEIKI 180

Query: 181 PKLEANQSAESKNKDSED 196
           PKLE NQ +ESK+KD+ D
Sbjct: 181 PKLETNQGSESKSKDNSD 197

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BLAST of MC10g1010 vs. NCBI nr
Match: XP_008463443.1 (PREDICTED: 21.7 kDa class VI heat shock protein [Cucumis melo] >KAA0025459.1 21.7 kDa class VI heat shock protein [Cucumis melo var. makuwa] >TYK04722.1 21.7 kDa class VI heat shock protein [Cucumis melo var. makuwa])

HSP 1 Score: 315 bits (808), Expect = 3.76e-107
Identity = 159/198 (80.30%), Postives = 176/198 (88.89%), Query Frame = 0


Query: 1   MATSKQLEVHREDH-TPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFFD 60
           MAT KQLEVH+EDH TPSKWSVSLGEE FRRFLGQAN  +QKVFGDGSLFSPLLFGKFFD
Sbjct: 1   MATPKQLEVHKEDHSTPSKWSVSLGEEVFRRFLGQANSAIQKVFGDGSLFSPLLFGKFFD 60

Query: 61  PADAFPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIENG-KV 120
           PADAFPLWEFESDLLLSNLR SGKS+IDW Q DQEY+LQAEL EA RN +QI IE G KV
Sbjct: 61  PADAFPLWEFESDLLLSNLRISGKSSIDWSQTDQEYILQAELLEAWRNALQISIEEGGKV 120

Query: 121 LEISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKNDMVIEVKI 180
           LEISGQL++Q QRE KT +DWR VNWWEHG+VRRLELP+DAD  RMEAR+KND+V+E+KI
Sbjct: 121 LEISGQLREQ-QREGKTTVDWRRVNWWEHGFVRRLELPEDADSSRMEARIKNDLVLEIKI 180

Query: 181 PKLEANQSAESKNKDSED 196
           PKLE NQS+ESK+KD+ D
Sbjct: 181 PKLETNQSSESKSKDNSD 197

[Close]

BLAST of MC10g1010 vs. NCBI nr
Match: XP_038905887.1 (21.7 kDa class VI heat shock protein [Benincasa hispida])

HSP 1 Score: 313 bits (803), Expect = 2.17e-106
Identity = 158/198 (79.80%), Postives = 175/198 (88.38%), Query Frame = 0


Query: 1   MATSKQ-LEVHREDH-TPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFF 60
           MATSKQ LEV +EDH TPSKW+V+LGE+ FRRFLGQ+N  VQKVF DGSLFSPLLFGKFF
Sbjct: 1   MATSKQQLEVRKEDHHTPSKWTVALGEDVFRRFLGQSNTAVQKVFSDGSLFSPLLFGKFF 60

Query: 61  DPADAFPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIENGKV 120
           DPADAFPLWEFESDLLLSNLR+SGKS+IDWFQ DQEYVLQAEL EA RN +QI IE+GKV
Sbjct: 61  DPADAFPLWEFESDLLLSNLRSSGKSSIDWFQTDQEYVLQAELLEAWRNALQISIEDGKV 120

Query: 121 LEISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKNDMVIEVKI 180
           LEISGQLK+Q QRE KTI+DWR VNWWEHGYVRRLELP+DADW RMEAR+KND V+E+KI
Sbjct: 121 LEISGQLKEQ-QREGKTIVDWRHVNWWEHGYVRRLELPEDADWSRMEARMKNDNVLEIKI 180

Query: 181 PKLEANQSAESKNKDSED 196
            K E NQ  ESK+KD+ D
Sbjct: 181 QKSETNQGVESKSKDNSD 197

[Close]

BLAST of MC10g1010 vs. NCBI nr
Match: ADU55792.1 (HSP23.0 [Citrullus lanatus])

HSP 1 Score: 312 bits (799), Expect = 8.83e-106
Identity = 157/198 (79.29%), Postives = 173/198 (87.37%), Query Frame = 0


Query: 1   MATSKQ-LEVHREDH-TPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFF 60
           MATSKQ LEVH+EDH TPSKWSVSLGEE FR+F+GQAN  VQ VFGDGSLFSPLLFGKFF
Sbjct: 1   MATSKQQLEVHKEDHHTPSKWSVSLGEEVFRKFVGQANSAVQTVFGDGSLFSPLLFGKFF 60

Query: 61  DPADAFPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIENGKV 120
           DPADAFPLWEFESDLLLSNLR SGKS++DWFQ DQEY LQAEL EA RN +QI IE+GKV
Sbjct: 61  DPADAFPLWEFESDLLLSNLRISGKSSVDWFQTDQEYGLQAELLEAWRNALQISIEDGKV 120

Query: 121 LEISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKNDMVIEVKI 180
           LEISGQLK+Q QRE +T  DWRSVNWWEHGYVRRLELP+DADW RMEAR+KND V+E+KI
Sbjct: 121 LEISGQLKEQ-QREGRTTFDWRSVNWWEHGYVRRLELPEDADWSRMEARMKNDHVLEIKI 180

Query: 181 PKLEANQSAESKNKDSED 196
           PK E N+  ES +KD+ D
Sbjct: 181 PKSETNEGVESNSKDNSD 197

[Close]

BLAST of MC10g1010 vs. ExPASy TrEMBL
Match: A0A6J1DNR7 (21.7 kDa class VI heat shock protein OS=Momordica charantia OX=3673 GN=LOC111022088 PE=3 SV=1)

HSP 1 Score: 395 bits (1015), Expect = 4.59e-139
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 0


Query: 1   MATSKQLEVHREDHTPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFFDP 60
           MATSKQLEVHREDHTPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFFDP
Sbjct: 1   MATSKQLEVHREDHTPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFFDP 60

Query: 61  ADAFPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIENGKVLE 120
           ADAFPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIENGKVLE
Sbjct: 61  ADAFPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIENGKVLE 120

Query: 121 ISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKNDMVIEVKIPK 180
           ISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKNDMVIEVKIPK
Sbjct: 121 ISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKNDMVIEVKIPK 180

Query: 181 LEANQSAESKNKDSED 196
           LEANQSAESKNKDSED
Sbjct: 181 LEANQSAESKNKDSED 196

[Close]

BLAST of MC10g1010 vs. ExPASy TrEMBL
Match: A0A0A0L6D5 (SHSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G081340 PE=3 SV=1)

HSP 1 Score: 317 bits (813), Expect = 3.15e-108
Identity = 161/198 (81.31%), Postives = 177/198 (89.39%), Query Frame = 0


Query: 1   MATSKQLEVHREDH-TPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFFD 60
           MATSKQLEVH+EDH TPSKWSVSLGEE FRRFLGQAN  +QKVFGDGSLFSPLLFGKFFD
Sbjct: 1   MATSKQLEVHKEDHPTPSKWSVSLGEEVFRRFLGQANSAIQKVFGDGSLFSPLLFGKFFD 60

Query: 61  PADAFPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIE-NGKV 120
           PADAFPLWEFESDLLLSNLR SGKS+IDW Q DQEYVLQAEL EA RN +QI IE +GKV
Sbjct: 61  PADAFPLWEFESDLLLSNLRISGKSSIDWSQTDQEYVLQAELLEAWRNALQISIEEDGKV 120

Query: 121 LEISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKNDMVIEVKI 180
           LEISGQLK+Q QRE KT +DWR V+WWEHGYVRRLELP+DAD  RMEAR+KND+V+E+KI
Sbjct: 121 LEISGQLKEQ-QREGKTTVDWRRVHWWEHGYVRRLELPEDADSSRMEARIKNDLVLEIKI 180

Query: 181 PKLEANQSAESKNKDSED 196
           PKLE NQ +ESK+KD+ D
Sbjct: 181 PKLETNQGSESKSKDNSD 197

[Close]

BLAST of MC10g1010 vs. ExPASy TrEMBL
Match: A0A5D3C0M7 (21.7 kDa class VI heat shock protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G00120 PE=3 SV=1)

HSP 1 Score: 315 bits (808), Expect = 1.82e-107
Identity = 159/198 (80.30%), Postives = 176/198 (88.89%), Query Frame = 0


Query: 1   MATSKQLEVHREDH-TPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFFD 60
           MAT KQLEVH+EDH TPSKWSVSLGEE FRRFLGQAN  +QKVFGDGSLFSPLLFGKFFD
Sbjct: 1   MATPKQLEVHKEDHSTPSKWSVSLGEEVFRRFLGQANSAIQKVFGDGSLFSPLLFGKFFD 60

Query: 61  PADAFPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIENG-KV 120
           PADAFPLWEFESDLLLSNLR SGKS+IDW Q DQEY+LQAEL EA RN +QI IE G KV
Sbjct: 61  PADAFPLWEFESDLLLSNLRISGKSSIDWSQTDQEYILQAELLEAWRNALQISIEEGGKV 120

Query: 121 LEISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKNDMVIEVKI 180
           LEISGQL++Q QRE KT +DWR VNWWEHG+VRRLELP+DAD  RMEAR+KND+V+E+KI
Sbjct: 121 LEISGQLREQ-QREGKTTVDWRRVNWWEHGFVRRLELPEDADSSRMEARIKNDLVLEIKI 180

Query: 181 PKLEANQSAESKNKDSED 196
           PKLE NQS+ESK+KD+ D
Sbjct: 181 PKLETNQSSESKSKDNSD 197

[Close]

BLAST of MC10g1010 vs. ExPASy TrEMBL
Match: A0A1S3CJA6 (21.7 kDa class VI heat shock protein OS=Cucumis melo OX=3656 GN=LOC103501614 PE=3 SV=1)

HSP 1 Score: 315 bits (808), Expect = 1.82e-107
Identity = 159/198 (80.30%), Postives = 176/198 (88.89%), Query Frame = 0


Query: 1   MATSKQLEVHREDH-TPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFFD 60
           MAT KQLEVH+EDH TPSKWSVSLGEE FRRFLGQAN  +QKVFGDGSLFSPLLFGKFFD
Sbjct: 1   MATPKQLEVHKEDHSTPSKWSVSLGEEVFRRFLGQANSAIQKVFGDGSLFSPLLFGKFFD 60

Query: 61  PADAFPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIENG-KV 120
           PADAFPLWEFESDLLLSNLR SGKS+IDW Q DQEY+LQAEL EA RN +QI IE G KV
Sbjct: 61  PADAFPLWEFESDLLLSNLRISGKSSIDWSQTDQEYILQAELLEAWRNALQISIEEGGKV 120

Query: 121 LEISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKNDMVIEVKI 180
           LEISGQL++Q QRE KT +DWR VNWWEHG+VRRLELP+DAD  RMEAR+KND+V+E+KI
Sbjct: 121 LEISGQLREQ-QREGKTTVDWRRVNWWEHGFVRRLELPEDADSSRMEARIKNDLVLEIKI 180

Query: 181 PKLEANQSAESKNKDSED 196
           PKLE NQS+ESK+KD+ D
Sbjct: 181 PKLETNQSSESKSKDNSD 197

[Close]

BLAST of MC10g1010 vs. ExPASy TrEMBL
Match: H6TB45 (HSP23.0 OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 312 bits (799), Expect = 4.27e-106
Identity = 157/198 (79.29%), Postives = 173/198 (87.37%), Query Frame = 0


Query: 1   MATSKQ-LEVHREDH-TPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFF 60
           MATSKQ LEVH+EDH TPSKWSVSLGEE FR+F+GQAN  VQ VFGDGSLFSPLLFGKFF
Sbjct: 1   MATSKQQLEVHKEDHHTPSKWSVSLGEEVFRKFVGQANSAVQTVFGDGSLFSPLLFGKFF 60

Query: 61  DPADAFPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIENGKV 120
           DPADAFPLWEFESDLLLSNLR SGKS++DWFQ DQEY LQAEL EA RN +QI IE+GKV
Sbjct: 61  DPADAFPLWEFESDLLLSNLRISGKSSVDWFQTDQEYGLQAELLEAWRNALQISIEDGKV 120

Query: 121 LEISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKNDMVIEVKI 180
           LEISGQLK+Q QRE +T  DWRSVNWWEHGYVRRLELP+DADW RMEAR+KND V+E+KI
Sbjct: 121 LEISGQLKEQ-QREGRTTFDWRSVNWWEHGYVRRLELPEDADWSRMEARMKNDHVLEIKI 180

Query: 181 PKLEANQSAESKNKDSED 196
           PK E N+  ES +KD+ D
Sbjct: 181 PKSETNEGVESNSKDNSD 197

[Close]

BLAST of MC10g1010 vs. TAIR 10
Match: AT5G54660.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 204.9 bits (520), Expect = 5.9e-53
Identity = 99/193 (51.30%), Postives = 138/193 (71.50%), Query Frame = 0


Query: 4   SKQLEVHREDHTPSKWSVSLGEEAFRRFLGQANPVVQKVFGDGSLFSPLLFGKFFDPADA 63
           S +LE+H +D TP KWSV LG++ FRRFL       + VFG+GSLFSP LFGK+FDP+DA
Sbjct: 7   SLKLEIHTDDKTPGKWSVPLGDDVFRRFLSGGGGSEKAVFGEGSLFSPFLFGKYFDPSDA 66

Query: 64  FPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYVLQAELPEAVRNTMQIFIE-NGKVLEIS 123
           FPLWEFE+++LL++LR+ G+  +DW Q DQ YVL++++P   +N +Q++++ NG+V+EIS
Sbjct: 67  FPLWEFEAEVLLASLRSLGQCRVDWSQTDQAYVLKSDIPVVGKNNVQVYVDINGRVMEIS 126

Query: 124 GQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWRRMEARVKND---MVIEVKIP 183
           GQ      +++ T  DWRS  WWEHGYVRRLELP DAD +  EA + N+     +E++IP
Sbjct: 127 GQ--WNSNKKAATNSDWRSGRWWEHGYVRRLELPSDADAKYSEAFLSNNDDYSFLEIRIP 186

Query: 184 KLEANQSAESKNK 193
           K+       SKNK
Sbjct: 187 KI------NSKNK 191

[Close]

BLAST of MC10g1010 vs. TAIR 10
Match: AT1G59860.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 57.4 bits (137), Expect = 1.5e-08
Identity = 41/158 (25.95%), Postives = 81/158 (51.27%), Query Frame = 0


Query: 38  VVQKVFGDGSLFSPLLFGKF-FDPADAFPLWEFESDLLLSNLRNSGKSTIDWFQIDQEYV 97
           ++   FG+    +  +F  F  D  D F   +F S    S+      + +DW +  + +V
Sbjct: 3   LIPSFFGNNRRINNNIFDPFSLDVWDPFKELQFPS----SSSSAIANARVDWKETAEAHV 62

Query: 98  LQAELPEAVRNTMQIFIENGKVLEISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELP 157
            +A+LP   +  +++ IE+  VL+ISG+   +++ +  T   W  V     G+ R+  LP
Sbjct: 63  FKADLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDT---WHRVERSSGGFSRKFRLP 122

Query: 158 DDADWRRMEARVKNDMVIEVKIPKLEAN-QSAESKNKD 194
           ++    +++A ++N  V+ V +PK+E N + A+ K+ D
Sbjct: 123 ENVKMDQVKASMENG-VLTVTVPKVETNKKKAQVKSID 152

[Close]

BLAST of MC10g1010 vs. TAIR 10
Match: AT3G46230.1 (heat shock protein 17.4 )

HSP 1 Score: 57.4 bits (137), Expect = 1.5e-08
Identity = 36/147 (24.49%), Postives = 74/147 (50.34%), Query Frame = 0


Query: 51  PLLFG----KFFDPADAFPLWEFESDLLLSNLRNS--------GKSTIDWFQIDQEYVLQ 110
           P  FG      FDP  +  +W+     L   L N+          + +DW +  + +V +
Sbjct: 5   PSFFGGRRTNVFDPF-SLDVWDPFEGFLTPGLTNAPAKDVAAFTNAKVDWRETPEAHVFK 64

Query: 111 AELPEAVRNTMQIFIENGKVLEISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDD 170
           A++P   +  +++ +E+G +L+ISG+   + + +S T   W  V      ++RR  LP++
Sbjct: 65  ADVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDT---WHRVERSSGKFMRRFRLPEN 124

Query: 171 ADWRRMEARVKNDMVIEVKIPKLEANQ 186
           A    ++A ++N  V+ V +PK++ ++
Sbjct: 125 AKVEEVKASMENG-VLSVTVPKVQESK 146

[Close]

BLAST of MC10g1010 vs. TAIR 10
Match: AT2G29500.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 54.3 bits (129), Expect = 1.3e-07
Identity = 38/144 (26.39%), Postives = 74/144 (51.39%), Query Frame = 0


Query: 47  SLFSPLLFGKFFDPADAFPLWEFESDLLLSNL--RNSG--KSTIDWFQIDQEYVLQAELP 106
           S F+       FDP  +  +W+   +L  S+L   NS    + +DW +  + +V +A+LP
Sbjct: 6   SFFNNNRRSNIFDPF-SLDVWDPFKELTSSSLSRENSAIVNARVDWRETPEAHVFKADLP 65

Query: 107 EAVRNTMQIFIENGKVLEISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLELPDDADWR 166
              +  +++ IE   VL+ISG+   +++ ++ T   W  V      + RR  LP++    
Sbjct: 66  GLKKEEVKVEIEEDSVLKISGERHVEKEDKNDT---WHRVERSSGQFTRRFRLPENVKMD 125

Query: 167 RMEARVKNDMVIEVKIPKLEANQS 187
           +++A ++N  V+ V +PK E  ++
Sbjct: 126 QVKAAMENG-VLTVTVPKAETKKA 144

[Close]

BLAST of MC10g1010 vs. TAIR 10
Match: AT1G07400.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 8.3e-07
Identity = 40/159 (25.16%), Postives = 81/159 (50.94%), Query Frame = 0


Query: 38  VVQKVFGDGSLFSPLLFGKFFDPADAFPLWEFESDLL--LSNLRNSGKSTIDWFQIDQEY 97
           ++   FG+    + +      D  D F   +F S L    S + N   + +DW +  + +
Sbjct: 3   LIPSFFGNNRRSNSIFDPFSLDVWDPFKELQFPSSLSGETSAITN---ARVDWKETAEAH 62

Query: 98  VLQAELPEAVRNTMQIFIENGKVLEISGQLKQQQQRESKTIIDWRSVNWWEHGYVRRLEL 157
           V +A+LP   +  +++ IE+  VL+ISG+   +++ +  T   W  V      + R+ +L
Sbjct: 63  VFKADLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDT---WHRVERSSGQFSRKFKL 122

Query: 158 PDDADWRRMEARVKNDMVIEVKIPKL-EANQSAESKNKD 194
           P++    +++A ++N  V+ V +PK+ EA + A+ K+ D
Sbjct: 123 PENVKMDQVKASMENG-VLTVTVPKVEEAKKKAQVKSID 154

The following BLAST results are available for this feature:

BLAST of MC10g1010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FIT9	8.3e-52	51.30	21.7 kDa class VI heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP21.7 ...	[more]
Q6AUW3	5.2e-38	42.70	22.3 kDa class VI heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]
P27396	2.5e-08	29.66	17.8 kDa class I heat shock protein OS=Daucus carota OX=4039 PE=3 SV=1	[more]
P27397	9.6e-08	30.93	18.0 kDa class I heat shock protein OS=Daucus carota OX=4039 PE=3 SV=1	[more]
P31673	1.2e-07	27.91	17.4 kDa class I heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]

BLAST of MC10g1010 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154944.1	9.47e-139	100.00	21.7 kDa class VI heat shock protein [Momordica charantia]	[more]
XP_004152915.1	6.50e-108	81.31	21.7 kDa class VI heat shock protein isoform X1 [Cucumis sativus] >KGN56157.1 hy...	[more]
XP_008463443.1	3.76e-107	80.30	PREDICTED: 21.7 kDa class VI heat shock protein [Cucumis melo] >KAA0025459.1 21....	[more]
XP_038905887.1	2.17e-106	79.80	21.7 kDa class VI heat shock protein [Benincasa hispida]	[more]
ADU55792.1	8.83e-106	79.29	HSP23.0 [Citrullus lanatus]	[more]

BLAST of MC10g1010 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DNR7	4.59e-139	100.00	21.7 kDa class VI heat shock protein OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A0A0L6D5	3.15e-108	81.31	SHSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G081340 PE=3 S...	[more]
A0A5D3C0M7	1.82e-107	80.30	21.7 kDa class VI heat shock protein OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3CJA6	1.82e-107	80.30	21.7 kDa class VI heat shock protein OS=Cucumis melo OX=3656 GN=LOC103501614 PE=...	[more]
H6TB45	4.27e-106	79.29	HSP23.0 OS=Citrullus lanatus OX=3654 PE=2 SV=1	[more]

BLAST of MC10g1010 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54660.1	5.9e-53	51.30	HSP20-like chaperones superfamily protein	[more]
AT1G59860.1	1.5e-08	25.95	HSP20-like chaperones superfamily protein	[more]
AT3G46230.1	1.5e-08	24.49	heat shock protein 17.4	[more]
AT2G29500.1	1.3e-07	26.39	HSP20-like chaperones superfamily protein	[more]
AT1G07400.1	8.3e-07	25.16	HSP20-like chaperones superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 184..196
None	No IPR available	PANTHER	PTHR47838:SF1	21.7 KDA CLASS VI HEAT SHOCK PROTEIN	coord: 1..184
None	No IPR available	PANTHER	PTHR47838	21.7 KDA CLASS VI HEAT SHOCK PROTEIN	coord: 1..184
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 88..188 e-value: 5.0E-8 score: 33.0
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 77..195 score: 11.517023
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 49..191 e-value: 2.7E-16 score: 61.6
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 51..186

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g1010.1	MC10g1010.1	mRNA