MC10g1000 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g1000
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionULP_PROTEASE domain-containing protein
LocationMC10: 9217855 .. 9218175 (-)
RNA-Seq ExpressionMC10g1000
SyntenyMC10g1000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTTACAACAATATGCATTTCTTCAACCATCATTGATTTCAACCGATTCAGGAAATGAGGAGAAACGATCTTTAATATTATGCAATCAATTAAGAGAGATCAACCATCGATTAGTTATCATTCCTTGTAATCCTGGGTAAGTATATATTTGAGTTATTAGTACATTTTTCTATTTGAATCGAGTATAAGTTTAATAGGTGATCATTGTATATAGATGTCATTGGATGCTGCTGGTTATATCGTTAGGGACTAGTACAATCTTTTCAATAGACTCTCTTGGACATGACATCCGCAATGAATTAAAGAATATAGTTAATACG

mRNA sequence

CTCTTACAACAATATGCATTTCTTCAACCATCATTGATTTCAACCGATTCAGGAAATGAGGAGAAACGATCTTTAATATTATGCAATCAATTAAGAGAGATCAACCATCGATTAGTTATCATTCCTTGTAATCCTGGATGTCATTGGATGCTGCTGGTTATATCGTTAGGGACTAGTACAATCTTTTCAATAGACTCTCTTGGACATGACATCCGCAATGAATTAAAGAATATAGTTAATACG

Coding sequence (CDS)

CTCTTACAACAATATGCATTTCTTCAACCATCATTGATTTCAACCGATTCAGGAAATGAGGAGAAACGATCTTTAATATTATGCAATCAATTAAGAGAGATCAACCATCGATTAGTTATCATTCCTTGTAATCCTGGATGTCATTGGATGCTGCTGGTTATATCGTTAGGGACTAGTACAATCTTTTCAATAGACTCTCTTGGACATGACATCCGCAATGAATTAAAGAATATAGTTAATACG

Protein sequence

LLQQYAFLQPSLISTDSGNEEKRSLILCNQLREINHRLVIIPCNPGCHWMLLVISLGTSTIFSIDSLGHDIRNELKNIVNT
Homology
BLAST of MC10g1000 vs. NCBI nr
Match: RVW70666.1 (hypothetical protein CK203_059004 [Vitis vinifera])

HSP 1 Score: 60.1 bits (144), Expect = 4.59e-09
Identity = 29/84 (34.52%), Postives = 50/84 (59.52%), Query Frame = 0

Query: 1  LLQQYAFLQPSLIST---DSGNEEKRSLILCNQLREINHR-LVIIPCNPGCHWMLLVISL 60
          ++ ++AF+ P+L+S       ++E RS ++ N+L   NH   + IP NPG HW+L+ +  
Sbjct: 6  MVGRFAFVNPTLVSKFGMGEASKESRSKVIANRLMNANHADFIFIPYNPGYHWVLVALET 65

Query: 61 GTSTIFSIDSLGHDIRNELKNIVN 80
           T   + +DSL +   ++LK IVN
Sbjct: 66 RTMIAYYLDSLQNQPSDDLKEIVN 89

BLAST of MC10g1000 vs. NCBI nr
Match: TYK02903.1 (transposase [Cucumis melo var. makuwa])

HSP 1 Score: 60.8 bits (146), Expect = 1.77e-08
Identity = 31/81 (38.27%), Postives = 47/81 (58.02%), Query Frame = 0

Query: 2   LQQYAFLQPSLISTDSGNEEKRSLILCNQLREIN-HRLVIIPCNPGCHWMLLVISLGTST 61
           L  Y F+ PSLIS     +E R+  LCN+L     ++LV+ P NPG HW LL I++   T
Sbjct: 615 LSNYIFIDPSLISVGHNTQEVRARNLCNRLMVSKPNQLVLAPFNPGGHWALLAINIYDET 674

Query: 62  IFSIDSLGHDIRNELKNIVNT 81
           ++ +DSL    R +++ +  T
Sbjct: 675 VYYLDSLRTTSRVDIRYVTYT 695

BLAST of MC10g1000 vs. NCBI nr
Match: KAA0051623.1 (uncharacterized protein E6C27_scaffold4784G00030 [Cucumis melo var. makuwa] >TYK27633.1 uncharacterized protein E5676_scaffold3445G00180 [Cucumis melo var. makuwa])

HSP 1 Score: 59.7 bits (143), Expect = 1.94e-08
Identity = 29/81 (35.80%), Postives = 48/81 (59.26%), Query Frame = 0

Query: 2   LQQYAFLQPSLISTDSGNEEKRSLILCNQLREI-NHRLVIIPCNPGCHWMLLVISLGTST 61
           L  Y F+ P LI+ +   +E R+  LC++L    +++LV+ P NPGCHW LL I+    T
Sbjct: 113 LSNYIFVDPFLINVEHNTQEVRARNLCSRLMASKSNQLVLAPFNPGCHWALLAINAYNET 172

Query: 62  IFSIDSLGHDIRNELKNIVNT 81
           ++ +DSL    R +++   +T
Sbjct: 173 VYYLDSLRTASRVDIRYSTDT 193

BLAST of MC10g1000 vs. NCBI nr
Match: ONH90473.1 (hypothetical protein PRUPE_8G055800 [Prunus persica])

HSP 1 Score: 60.1 bits (144), Expect = 2.06e-08
Identity = 30/78 (38.46%), Postives = 52/78 (66.67%), Query Frame = 0

Query: 7   FLQPSLISTDSGNEEKRSLILCNQLREIN-HRLVIIPCNPGCHWMLLVISLGTSTIFSID 66
           F+ P+ +S +SG   +RS ++  +L++ +  +L ++P NPGCHW+LL++     TI+ +D
Sbjct: 83  FIDPATVSANSGTITERSRLVAARLQKTDGEQLFMMPYNPGCHWILLIVRAKKETIYFLD 142

Query: 67  SL-GHDIRNE-LKNIVNT 81
            L GH + +E  KNIVN+
Sbjct: 143 PLPGHRVVDEEAKNIVNS 160

BLAST of MC10g1000 vs. NCBI nr
Match: XP_010647538.1 (PREDICTED: uncharacterized protein LOC104878639 [Vitis vinifera])

HSP 1 Score: 58.9 bits (141), Expect = 2.68e-08
Identity = 29/81 (35.80%), Postives = 47/81 (58.02%), Query Frame = 0

Query: 4   QYAFLQPSLIST---DSGNEEKRSLILCNQLREINHR-LVIIPCNPGCHWMLLVISLGTS 63
           ++AF+ P+L+S       ++E RS ++ N+L   NH   + IP NPG HW+L+ +   T 
Sbjct: 107 RFAFVNPALVSKAGMGEASKESRSRVIANRLMNANHADFIFIPYNPGYHWVLVALETRTM 166

Query: 64  TIFSIDSLGHDIRNELKNIVN 80
             + +DSL     ++LK IVN
Sbjct: 167 IAYYLDSLEDQPSDDLKEIVN 187

BLAST of MC10g1000 vs. ExPASy TrEMBL
Match: A0A438GEN0 (ULP_PROTEASE domain-containing protein OS=Vitis vinifera OX=29760 GN=CK203_059004 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.22e-09
Identity = 29/84 (34.52%), Postives = 50/84 (59.52%), Query Frame = 0

Query: 1  LLQQYAFLQPSLIST---DSGNEEKRSLILCNQLREINHR-LVIIPCNPGCHWMLLVISL 60
          ++ ++AF+ P+L+S       ++E RS ++ N+L   NH   + IP NPG HW+L+ +  
Sbjct: 6  MVGRFAFVNPTLVSKFGMGEASKESRSKVIANRLMNANHADFIFIPYNPGYHWVLVALET 65

Query: 61 GTSTIFSIDSLGHDIRNELKNIVN 80
           T   + +DSL +   ++LK IVN
Sbjct: 66 RTMIAYYLDSLQNQPSDDLKEIVN 89

BLAST of MC10g1000 vs. ExPASy TrEMBL
Match: A0A5A7U6T9 (ULP_PROTEASE domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3445G00180 PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 9.38e-09
Identity = 29/81 (35.80%), Postives = 48/81 (59.26%), Query Frame = 0

Query: 2   LQQYAFLQPSLISTDSGNEEKRSLILCNQLREI-NHRLVIIPCNPGCHWMLLVISLGTST 61
           L  Y F+ P LI+ +   +E R+  LC++L    +++LV+ P NPGCHW LL I+    T
Sbjct: 113 LSNYIFVDPFLINVEHNTQEVRARNLCSRLMASKSNQLVLAPFNPGCHWALLAINAYNET 172

Query: 62  IFSIDSLGHDIRNELKNIVNT 81
           ++ +DSL    R +++   +T
Sbjct: 173 VYYLDSLRTASRVDIRYSTDT 193

BLAST of MC10g1000 vs. ExPASy TrEMBL
Match: M5VIC6 (ULP_PROTEASE domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_8G055800 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 9.99e-09
Identity = 30/78 (38.46%), Postives = 52/78 (66.67%), Query Frame = 0

Query: 7   FLQPSLISTDSGNEEKRSLILCNQLREIN-HRLVIIPCNPGCHWMLLVISLGTSTIFSID 66
           F+ P+ +S +SG   +RS ++  +L++ +  +L ++P NPGCHW+LL++     TI+ +D
Sbjct: 83  FIDPATVSANSGTITERSRLVAARLQKTDGEQLFMMPYNPGCHWILLIVRAKKETIYFLD 142

Query: 67  SL-GHDIRNE-LKNIVNT 81
            L GH + +E  KNIVN+
Sbjct: 143 PLPGHRVVDEEAKNIVNS 160

BLAST of MC10g1000 vs. ExPASy TrEMBL
Match: A0A5A7VCM5 (ULP_PROTEASE domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold548G00390 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.53e-08
Identity = 29/80 (36.25%), Postives = 46/80 (57.50%), Query Frame = 0

Query: 2   LQQYAFLQPSLISTDSGNEEKRSLILCNQLREINH-RLVIIPCNPGCHWMLLVISLGTST 61
           + +Y F+ PSLIS      E R+  LC++L      +LV+ P NPG HW L+VI+     
Sbjct: 245 VSKYVFIDPSLISAGHSTREIRARNLCSRLMTSKQDQLVVAPYNPGDHWSLVVINSYDDV 304

Query: 62  IFSIDSLGHDIRNELKNIVN 80
           ++ +DSL    R+++K + N
Sbjct: 305 VYHLDSLRTSFRDDIKYVTN 324

BLAST of MC10g1000 vs. ExPASy TrEMBL
Match: A0A5D3DYN7 (ULP_PROTEASE domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2030G00320 PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.55e-08
Identity = 29/80 (36.25%), Postives = 46/80 (57.50%), Query Frame = 0

Query: 2   LQQYAFLQPSLISTDSGNEEKRSLILCNQLREINH-RLVIIPCNPGCHWMLLVISLGTST 61
           + +Y F+ PSLIS      E R+  LC++L      +LV+ P NPG HW L+VI+     
Sbjct: 284 VSKYVFIDPSLISAGHSTREIRARNLCSRLMTSKQDQLVVAPYNPGDHWSLVVINSYDDV 343

Query: 62  IFSIDSLGHDIRNELKNIVN 80
           ++ +DSL    R+++K + N
Sbjct: 344 VYHLDSLRTSFRDDIKYVTN 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
RVW70666.14.59e-0934.52hypothetical protein CK203_059004 [Vitis vinifera][more]
TYK02903.11.77e-0838.27transposase [Cucumis melo var. makuwa][more]
KAA0051623.11.94e-0835.80uncharacterized protein E6C27_scaffold4784G00030 [Cucumis melo var. makuwa] >TYK... [more]
ONH90473.12.06e-0838.46hypothetical protein PRUPE_8G055800 [Prunus persica][more]
XP_010647538.12.68e-0835.80PREDICTED: uncharacterized protein LOC104878639 [Vitis vinifera][more]
Match NameE-valueIdentityDescription
A0A438GEN02.22e-0934.52ULP_PROTEASE domain-containing protein OS=Vitis vinifera OX=29760 GN=CK203_05900... [more]
A0A5A7U6T99.38e-0935.80ULP_PROTEASE domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
M5VIC69.99e-0938.46ULP_PROTEASE domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_8G0558... [more]
A0A5A7VCM51.53e-0836.25ULP_PROTEASE domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A5D3DYN71.55e-0836.25ULP_PROTEASE domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003653Ulp1 protease family, C-terminal catalytic domainPFAMPF02902Peptidase_C48coord: 38..77
e-value: 7.6E-5
score: 22.7
NoneNo IPR availableGENE3D3.40.395.10Adenoviral Proteinase; Chain Acoord: 5..81
e-value: 4.3E-8
score: 34.8
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 4..80

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g1000.1MC10g1000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008234 cysteine-type peptidase activity