MC10g0998 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g0998
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	gibberellin 2-beta-dioxygenase 8-like
Location	MC10: 9188769 .. 9196497 (+)
RNA-Seq Expression	MC10g0998
Synteny	MC10g0998

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTATGGAAATTGAACCTCCATTACAAGTTTTCTATAATGCCCTCATGAAGAAAGATGGTGAGGAGGGTGAAAATGGAAGAATATTGGAGGATGAAAGGGAACTAGTGGTGGAGGAATGTGATGAGCTACCACTTATTGATTTAGGGCTTCTAAAAATGGGGAATTTAGAGAGAGAGAAGTGCAAAAGGCAGATTGTTGAAGCAGCAAGAAATTGGGGTTTTTTTCAAGTGGTGAATCATGGGGTTCCAGAGAGGGCATTGAAGGCAGTGATGTTTGAGCAAAAGAAAGTGTTCAATCAGCCTTTTGAGAAGAAAAGTTTGAGAAATTTTATGAATTTGAGTGGGAGTTACCGCTGGGGGAACTCACAGGCCATTTCCCCAACCCAAATCTCTTGGTCTGAGGCTTTCCATATTGCTGTTTTGGAGGTTCCAAGCTTGGAGGACCAATATTTGAGCCTCAGGTATACTCACCCTAGCTAGATTCAGCTCAATTTTTTTCTATTAATTTGGTTTTCTTTTATGGATTTTGCTTGCTTTGGATGACTCTTTCTTCTTTTCTGCAAAAAAAAAAAAAAATTTAAAAATAGTTTTTTTTTTTATAATCCGGAATCGATGCCATGTAGCTTTACTAGTTGGATCCATATAACCAGGGTATCGACGGCCACCTAACACTGCAATAGACGTTGTGGAATTCGTCCCTGAATTTAGAGGGGAGGGACGAAAGCTCGGCTACGCACCTTGCAGGGCTCGAATTGGGGAGGGGTATCCCCATTCGGTCACAAACCCACTATGCCACCACCTACTGGTGGAATTTTTTTTTTTTTAAATATTCAAACTTTTATATTTTTACTAACCTCTCTGGTCAGCTTATGGGAAAATTTTGTGGCTTGATAAAGATTATTTGTAAGTAATAGGGTTGATAAATAGTGAAACAAGTAATATTAGGGTTTTTTTGTTTAAAGAAAATTAAGTCTAATCACAAGAATAAATCATCTTTAAAGTCACCCTTTCATCTTGTGCTATGATATTTTTGTCATGATTTATCAACAGATGAAGAACATAGCCATAGTTAGATTAGGTTTCGCTAATTAGGTATATAATCAGAAGACAAATTAACTTGGGAATTACTCCTCCTGTTTCACATTAAATTATCTAACCACAGTTAATCAGAGAGAGAGAAAAATTGGCCTCAAAGAAATGAAAATATACTAGATCACAAGGTTAATTATATATATATATATAGCACATTTAAACTTTGATTAGAGAATGAAAACTCCAAAATTTCTTTTAATTGACTAGAAGTACAAGATTCTAGATAACTGTAGAAGCTATAGGAGTAATTCATTATGACGAAGAAAATATATGTGGCTAAATGTATAAGATTCTCTACTAATATATAGAATATAAAGTAATTGCATTATGAAGAAGATGGTGTGGTGGTTACACAACCAATTAGATTAAAAGCCTATAAATATGTTATACTTGTATAATGGGGTAACCTTTGGGAAGTTTGAGTATTCTTTTGGTAAATTTATATATATATTGTTGGAATGCTTATGTTTTATGAAGCTATGTTAAAAAGTTTAATGTGCATGATTTCTATTGTGTTTTAGTACAGTAAGTAGGGTGGACGGCTTTGAACTTACGACTTTCTAGTCCACATAGGTGTTTTAATCCGTTGAACTATGCTCAGGTTAGCTTGTTCATATTGTTCAACATTGTTTATTTTTATTTTTATTTTCTTTTTCTTTCTGCCCAATAAAATACTTGAAAAGTTGAAATATTTACTATCCTATGGTTAATAGGCTCTTTGCATTGAAAAGAAAAACAAATTTCATTCCAAAAAATTGAGAACAATTTTGCTACCCAACTTTCCAAATAGTTTCCCTCATAATTATTGTGAAAAATATCGTGTATTAAATTTTATTTAGGTCCTTCATCTTACTAAAATGAAGGTAAGGATGAAACATATTTGTTACCTCTTTAGGAGTACGTGGAAAACACACAAAAAGGAGACTAAACAAAAACAATTCAATTTAGCGAAATGTTAGATACATCATATAATTACGAAAGGGGAAGAATTTATAAAACTAAGAGGAGACTTAGACTTTAATATCTTTCCAAAGTTGAGTATAAGATCTGGATCTATCCATAAAGGAAAGTCAACTCTGATAACCAGTAGGAAACAATCAAAATCTTTTAAGAGTAATTTCTAGATGTATATCATTTAGGAGTATATTTTTATCAACTCACAACTTTAGTTTATTTTTTTTACTTCCTTCTAAGCCATCAGAAGATCTCATGTTCAATTAAAAACTCGATCTTAAAAGAATTATTATTATTTGTTTTTTATTATTACTACTACTACTAGTACAATTTTTGAAAATGATAAATCAAAGTAGGGTATAGCTAGAAGTGAGCAATCCCAATAAGCTACTACTCTATTTCTCTCTCTTGCCTTTTTCCCCTAAAGAAGCAACAAAAACTACATACTCTCTCATATATCAACACACAAGAGTACTTTTTTCTTTCTCTATCTCTTGATTGGAAGTTTTGAATAGTATTTTTTTACCCTTTCACTTGCTTCTTTGGTTTTGAATTCAATGTCTGAATTTACTTCAAGTACCAAAACGAAAAAGTACTTTCATACAGCTCATTTAAACTTACAAAAACATTAATAACAAAATAGAGAAAAATATCACAATCATGCATAGGTTTGGCTATGTTAGACGAATAGTCCAAGTTCATGAGCAATAGAGCGAGTTGAAAAAGCTTCGGTTTGTGTACCTCATGTCTGAGCGAGTTCCAAATGACTCTTGAGAATAGTACTTTTTAAGTGAGAAATTTAAAACCCTTTTAGAAGAAGTATTATTAGAAAAACAATTTTTAATGTTTTTAAGCAATCTTATACTTGACTATATACAAAACTCACCCTAAATTTTCCTTTATAAAAGTTAGCTTTCTCACTCAAGGAGGGGAAAAAACTATTGAACGAGACTGTCCCTAATAACTATTGGATACAAATTCAAAATTTGTATATCCTTAACAACTATTTGACATAAATTCAAAACCACATTTTGTAGTTTTAAAAGTGTAAAACTACGGGTTACATTGTGATGGCTTCCTCTAATAATTTTTCCAAAAAGTGAATTAACTCTAAATCATAGTTTCGTAAAGAACTCTCTTGGATAAGGTTTTATATATATGCACTTTTAGCAGCTTTTATGGATGGATGACATAAGTTACTGTTTTGTCCAAGAAATATACATTTTCTTTTATTTTTGTTGTTGTTTTCTGGTTTTTAACATACCCCACTTGGACTGAAATGGCATGTGAAGTAAGTGAGAAGGGGTGCTTCCACAACAACAAAGTGCATGGCTCCATAGCCAATTCTCTTAGTTTGACACCACCCCATCACGTGACAATCACATGCACAAGTTTCCTAAAGGGCACAGCCTTCCATTATCTTATCGCAACACATTCATTTTGGCTCTTACTGCTCTCTCCTCTTTCATTATTGCTCCCCAGATATACTTTGTTTCCCAAACAGATGACAAAAACAACTTCCCATTTTCCCCATGTGTGTTTTCACATTTATTTTCTCAGACCTAAAAATCTCTTGCTCCCCCCTCTTTATCTGGTTTTTTTAAGATTCATTTGTGTATGTTAGATTCAGAGTAGTGTTTTTATGTACAACATGTTTTTTTAAATGTTTTTTTTTTCCAAAGTTTGGGAGGAAGGATTTTTATAATCTAACCAAACACTAGGAACATACTCATTAGTTGTTTATTATGTGCTAACCTACTTTCGATCTCAATCTTTTTTTTTTTTTGTTACATATATAGGATTCTGAATGGTCTTGTTCTGATATAAGCAACAAGATCTTATGTTAGACAATGAATTCTCTTATAAAGGGAGAAGTGAATTTTTTGAGATACATATGATGTCGGCTCTAAAATGGGACACTTTCAAATATTTCAAAAATATTTAGTAAAATTAATAAAATAACAATTATGAAGTCCAATTTTAAAATGACAACTTTACTCAAATGATATACATGTATTATATATCTGTAATATATCTAACTACTAATATATTTATATGATATATAATATGATACATTACTGATAAGAGTGTTTGATATACATACAATATGTATTTGATATACAACTAATATACTATTGTATGAGTGTCTTACTAGTGAAAATTTTTATTTATATTCTAAAACTTAAAAAAATAGGTGAGATATTTTGAAATAATGTTTTATTATCCACTGCAATTTTCGTAATGATTGTGACAAAAGTAGAGCAGCTTCTTGTGTCGTAGAAACAAAACAAAATTAACCCATTTGTTTGTTTGTGTGACAGATCAACAGTTGAAGGAGTGGTGAAGAAATTTGGGGGCTTAGCAGAGAGCATAGCAGAGATTTTAGGGCAAAGTTTGGGCATGAAATCGAGTTACTTCGAAGAAAGCTGCAAGAGAGGAAGGAGCAGCTTTCGGCTGAACAGATACCCACCTTGTCCCTTCGCCTCCAAAGTCTATGGCCTCGTTCCTCACACAGACACCGATTTTCTCACCATTCTTTATCAACCTCCTCAAATCAACGCTCTTCACCTCATGAAAGATGGCAACTGGCTTGCCGTCAAGCCTAACCCACAAGCCTTGCTTGTCAATATCGGCGACTTACTTCAGGTACTCCCATCCTGCCCCCATTTTTCAGTTTCCTCCAGTTTTAACTACGTTCTTGAACTTTTGATTATGATTCTAAATGGGTTCTTAGTGACCACATGTTGATTGGGCTAATGATGTTAAGGATTTCATGTTAAAAAGATAAAAAGGATATGACAAATCTAACAAGGACTCACAATAAGTTTTGAGATGGAATCTCATAGTATCAAAGTCCGAAAGCTCGAACGAGCATTCGGTTAAAAAATGGGATCCGACTAGTCAAAAGAGCTACGATCTTGAATATAAGTGGATGTTAAGGATCCTACGTTCAAAAGAGAGGGGATCTAACATAGGTTTATAATAGACATAGACTATTCCTCTAATGGAGATTCCAAAATGTTCAAATAGTACAATCAGTCTAAAAATGAGATTTAACCAAGATCGATCAGTCAAAAGAGTCACAATTTTCATTGTCAATTGGTTTTGAAATGGAACTCATGAATATGGATAGTGGACTACTCCTCCCATTAACAATTTATAATAAGTCATGATTTTGTTTGATATGAAAAGAACGATAAAAAAGAATAGGCTCTGGGCAAGCTATAAAAACATTTTGATTTAAAGAATATCTTCGACATAATTAAGTATCTCTTGAAATTTTTGGCCCACCCGCCAATATTTTATTTAGTTGGTGTACTCTTTTATGTTATGTTAGGTTAAATCATTTAGTTTGTTAGTCAAATTAACATGTCATGTTAGTGCATTTAAATAACGATATAATAATTTTACTAGAATCGAAATTGAAAAATTTAGACACATGAAATTTAATGATTAAATAGATACAATAATTAAGGTTCATGGATTAAATTTATTATTTAATTTATTTTTACAAGTATATTAAGCGTATATTTAAAGAATTAAAGATATTATAACCTATTAAATTTTATTTTTCCAGACTTAAGTAGTTTTTTAAACTTGATATCAAATTAAGAGATTCTCATATTTAAACTTTAAAATGTTATTTCTATGGTGAAACCTAACATAGTTCAAATAGTTAAGACATCTATCTTTGACCATGATAGAGTTCAAACTCCCATCTCCACAATGTTGTACTAAAAATTCTAAAAAATAATTAAGCTTCTCTCATGAACAACTTTGTTGGTTATATAATCTCTTTTTTTCGAACACTTTTTTTTTTTTTTTTGAAGTCTTAACCAAACTTTAAAAACTAGAAAAGTAATTTTTAAAAATTATTTTTTTAAAAAGAAGTAAGATTTCAAAAGTATTTTAAAAATATGAAAAATAAAACATACAGAAGATGTTAGAAAAGAAGATCAACGTTTTAAAGCCAAAAAAGGAAAAAAAACAAAAAACAAAATTGCTATCCATTAATACCAAGACTTTTTTCTAATTTTGTTTACAATTTCAGTGACAAACAAAAAAAAAATATTATCAAAAAAAATGAAAACAAAAATCTTAGTCCTTTGGTAACAATTCAATTTTAGTTTTTATTTTAAAAATTACTCTAGTTAATTAACCCTTTCCTTTTTTAATTTTCAAATTCTTAAAAACAATTTAAAAATTTTATTCAAATTTCAAACATTAAATAAAGTTTATTGAAAACTATATATTTTAGGATTTTTAAAATTTGGCTAGGCTTTTAAAAACATTCTTCAAAATAGATTAAAAGAACAAAAAGGAAAATTAATTAGTAACCAAAATATACAATCTATAAAAACATAAGACAAAAACAAAACTTTTAAGGATATATAATGAAATATCAATTGAGCCCTAGACTTATATCATGCTGGGATGTTTAAAAAAGTGAGAGAACAGTTACATAAACTTGAAAAAAATTTGATACTATACTTCACATATAGATTTCAAAGAACTTTATCTCCCTCGAGAATTAGCCAAATTGTATGTTAATAAAGGGTAGAAAAAGCTATGGTCTCCTTTGATAATGATTATATTTTTAGTTTTTTGGCTTAAAAAGTTCTTCATATGTTTATTGTTCATTAGGATCTCATGGAACTTAACAAAGTTTATAAGATGCCTAAGCTAGCTTTTTTTTTTTTTTCGCTAGTCAATTGATTTGAGATAAAGAATCTTGATATGGTATATGAGGAGAGATCCTATATTGTCATTTTCCCTTCTTTAATATTGATTTTGTCTTTCACAAGGGGTGAGTGTTAACATATATCTATGATTAAATTTACTTTAACCCATAAGCTTAAGCTTCTTGACTCATTGGTGATGAAATCTGATTAAGATTGGTGAGTCCAAAAGAACCATCATCTTAAGGGGTATATGTTACAAATCCTACACCAAAAATATCAAAGAGCCTAACAAAAGATATACATGGGTAGATATTTATCCCTTTTTTTTTAGAATTGGAAGTCAGAACTTTGCTCCTCTATACTAGGGACACTGTGCCCGTCCTAAGGCCCTGACCCGAGAGACAATCAAAGATTTTTGGTATTAAGTTCACCAGAAGGTTTGAACTTCAGACCTCTGAGTCAATATAAACCAGATGTTAAAGGATCTCACATTGAAAAAAAAACCTTACAATGATTTATAATAAACATGGGGTACTTCTCTCGTCGTCATTTGATTTTGAAATGGAATCCCATGAATATTGATATCATATACTCCAAGTCCTTATCAATCGGACTGCCCCTTGAGGACGATATTAAAAACACGGATAACAAAACAACAAACGGGATGTATATAATGAAAAAACTTAAATATCACCGACCTTTATGAAGAGAGAGTTCATGTTTTGCTGAAAAGTGGAGTGACAAAAATCTGACATATGCAGGTTGTGAGCAACGATGTGTTCAAGAGCCTGAAGCACAGAGTGATAGCTTCAGAAGCAGTTGAAAGATTTTCGTTTGCATATTTCTACTGCCCATCTGATGATGTGATGATTGAGAGCTGCACGAAACCAAGCATCTACAGGCAGTTCAGCTACAAAGAGTATAGACAACAAATAGAGAAAGATGTAGAGAAAACAGGCGACAAAGTAGGCCTTCCAAGGTTTCTCTTGCACAACATCATG

mRNA sequence

AGTATGGAAATTGAACCTCCATTACAAGTTTTCTATAATGCCCTCATGAAGAAAGATGGTGAGGAGGGTGAAAATGGAAGAATATTGGAGGATGAAAGGGAACTAGTGGTGGAGGAATGTGATGAGCTACCACTTATTGATTTAGGGCTTCTAAAAATGGGGAATTTAGAGAGAGAGAAGTGCAAAAGGCAGATTGTTGAAGCAGCAAGAAATTGGGGTTTTTTTCAAGTGGTGAATCATGGGGTTCCAGAGAGGGCATTGAAGGCAGTGATGTTTGAGCAAAAGAAAGTGTTCAATCAGCCTTTTGAGAAGAAAAGTTTGAGAAATTTTATGAATTTGAGTGGGAGTTACCGCTGGGGGAACTCACAGGCCATTTCCCCAACCCAAATCTCTTGGTCTGAGGCTTTCCATATTGCTGTTTTGGAGGTTCCAAGCTTGGAGGACCAATATTTGAGCCTCAGATCAACAGTTGAAGGAGTGGTGAAGAAATTTGGGGGCTTAGCAGAGAGCATAGCAGAGATTTTAGGGCAAAGTTTGGGCATGAAATCGAGTTACTTCGAAGAAAGCTGCAAGAGAGGAAGGAGCAGCTTTCGGCTGAACAGATACCCACCTTGTCCCTTCGCCTCCAAAGTCTATGGCCTCGTTCCTCACACAGACACCGATTTTCTCACCATTCTTTATCAACCTCCTCAAATCAACGCTCTTCACCTCATGAAAGATGGCAACTGGCTTGCCGTCAAGCCTAACCCACAAGCCTTGCTTGTCAATATCGGCGACTTACTTCAGGTTGTGAGCAACGATGTGTTCAAGAGCCTGAAGCACAGAGTGATAGCTTCAGAAGCAGTTGAAAGATTTTCGTTTGCATATTTCTACTGCCCATCTGATGATGTGATGATTGAGAGCTGCACGAAACCAAGCATCTACAGGCAGTTCAGCTACAAAGAGTATAGACAACAAATAGAGAAAGATGTAGAGAAAACAGGCGACAAAGTAGGCCTTCCAAGGTTTCTCTTGCACAACATCATG

Coding sequence (CDS)

AGTATGGAAATTGAACCTCCATTACAAGTTTTCTATAATGCCCTCATGAAGAAAGATGGTGAGGAGGGTGAAAATGGAAGAATATTGGAGGATGAAAGGGAACTAGTGGTGGAGGAATGTGATGAGCTACCACTTATTGATTTAGGGCTTCTAAAAATGGGGAATTTAGAGAGAGAGAAGTGCAAAAGGCAGATTGTTGAAGCAGCAAGAAATTGGGGTTTTTTTCAAGTGGTGAATCATGGGGTTCCAGAGAGGGCATTGAAGGCAGTGATGTTTGAGCAAAAGAAAGTGTTCAATCAGCCTTTTGAGAAGAAAAGTTTGAGAAATTTTATGAATTTGAGTGGGAGTTACCGCTGGGGGAACTCACAGGCCATTTCCCCAACCCAAATCTCTTGGTCTGAGGCTTTCCATATTGCTGTTTTGGAGGTTCCAAGCTTGGAGGACCAATATTTGAGCCTCAGATCAACAGTTGAAGGAGTGGTGAAGAAATTTGGGGGCTTAGCAGAGAGCATAGCAGAGATTTTAGGGCAAAGTTTGGGCATGAAATCGAGTTACTTCGAAGAAAGCTGCAAGAGAGGAAGGAGCAGCTTTCGGCTGAACAGATACCCACCTTGTCCCTTCGCCTCCAAAGTCTATGGCCTCGTTCCTCACACAGACACCGATTTTCTCACCATTCTTTATCAACCTCCTCAAATCAACGCTCTTCACCTCATGAAAGATGGCAACTGGCTTGCCGTCAAGCCTAACCCACAAGCCTTGCTTGTCAATATCGGCGACTTACTTCAGGTTGTGAGCAACGATGTGTTCAAGAGCCTGAAGCACAGAGTGATAGCTTCAGAAGCAGTTGAAAGATTTTCGTTTGCATATTTCTACTGCCCATCTGATGATGTGATGATTGAGAGCTGCACGAAACCAAGCATCTACAGGCAGTTCAGCTACAAAGAGTATAGACAACAAATAGAGAAAGATGTAGAGAAAACAGGCGACAAAGTAGGCCTTCCAAGGTTTCTCTTGCACAACATCATG

Protein sequence

SMEIEPPLQVFYNALMKKDGEEGENGRILEDERELVVEECDELPLIDLGLLKMGNLEREKCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGSYRWGNSQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILGQSLGMKSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALHLMKDGNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDDVMIESCTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM

Homology

[Close]

BLAST of MC10g0998 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 318.5 bits (815), Expect = 8.9e-86
Identity = 167/342 (48.83%), Postives = 225/342 (65.79%), Query Frame = 0


Query: 4   IEPPLQVFYNALM-----KKDGEEGENGRILEDERELVVEECDELPLIDLG-LLKMGNLE 63
           ++PP    YN L+     KKD +  E           VVEE  ELP+ID+  L+     E
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIP--FSFSVTAVVEEV-ELPVIDVSRLIDGAEEE 60

Query: 64  REKCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGSY 123
           REKCK  I  A+R WGFFQV+NHG+    L+ +  EQ +VF +PF+KKS     + +GSY
Sbjct: 61  REKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEKFS-AGSY 120

Query: 124 RWGNSQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILGQ 183
           RWG   A S  Q+SWSEAFH+ + ++   +D + +L ST+E    +   LA  +AE+L +
Sbjct: 121 RWGTPSATSIRQLSWSEAFHVPMTDISDNKD-FTTLSSTMEKFASESEALAYMLAEVLAE 180

Query: 184 SLGMKSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALHL 243
             G  SS+F+E+C R     R+NRYPPCP  S+VYGL+PHTD+DFLTILYQ  Q+  L L
Sbjct: 181 KSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQ-DQVGGLQL 240

Query: 244 MKDGNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDDV 303
           +KD  W+AVKPNP+AL++NIGDL Q  SN ++KS++HRV+ +  VERFS AYF CPS D 
Sbjct: 241 IKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDA 300

Query: 304 MIESCTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLH 340
           +IE  +    YR FS++E+RQQ+++DV+K G KVGLPRFL H
Sbjct: 301 VIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFLNH 336

[Close]

BLAST of MC10g0998 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 9.9e-77
Identity = 146/299 (48.83%), Postives = 203/299 (67.89%), Query Frame = 0


Query: 42  ELPLIDLGLLKMG-NLEREKCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQ 101
           +LP+IDL  L  G  ++R++C +Q+V AA+ WGFFQ+VNHG+P+   + ++ E+KK+F+Q
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 102 PFEKKSLRNFMNLS-GSYRWGNSQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEG 161
           PF  K    F +LS  SYRWGN  A SP Q S SEAFHI + EV  + D   +LR+ VE 
Sbjct: 99  PFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDRNNLRTIVET 158

Query: 162 VVKKFGGLAESIAEILGQSLGMKSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTD 221
            V++   +A+ I EILG+ + + S YFE   +   S  RLN+Y P  F S+V+GLVPHTD
Sbjct: 159 YVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTD 218

Query: 222 TDFLTILYQPPQINALHLMKDGNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIAS 281
           T FLTIL Q  QI  L L  +G W++VKP  +AL VNIGD+ Q +SN V++S++HRVI+ 
Sbjct: 219 TSFLTILSQ-DQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISP 278

Query: 282 EAVERFSFAYFYCPSDDVMIESCTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLL 339
             +ER S A+F CP  +  I+    P  YR+FS++EY++Q E DV++TGDKVGL RFL+
Sbjct: 279 ANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFLI 336

[Close]

BLAST of MC10g0998 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 5.6e-72
Identity = 145/360 (40.28%), Postives = 207/360 (57.50%), Query Frame = 0


Query: 4   IEPPLQVFYN--ALMKKDGE----------EGENGRILEDERELVVEECDELPLIDLGLL 63
           I+PPL   Y   AL+++D +           G  G +LE           +LP++DL  L
Sbjct: 9   IDPPLADSYRALALLRRDRDGGIAPPAVQMVGSGGAVLE----------RDLPMVDLERL 68

Query: 64  KMGNL-EREKCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNF 123
             G   ER+ C   +  AA  WGFFQ+ NHGV    ++ +  EQ ++F  PFE K     
Sbjct: 69  TRGGAGERKACAGAMARAASEWGFFQLTNHGVGRELMEEMRREQARLFRLPFETKEKAGL 128

Query: 124 MNLSGSYRWGNSQAISPTQISWSEAFHIAVLEVPSLEDQY---LSLRSTVEGVVKKFGGL 183
           +N  GSYRWGN  A S   +SWSEAFH+ +  +   +  +    SLR  ++ V +    +
Sbjct: 129 LN--GSYRWGNPTATSLRHLSWSEAFHVPLASISGADCDFGDLTSLRGVMQEVAEAMSRV 188

Query: 184 AESIAEILGQSL-------GMKSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDT 243
           A ++A  L + L          + +F   C       RLNRYP CPFA+  +GLVPHTD+
Sbjct: 189 ANTVAAALAEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACPFAADTFGLVPHTDS 248

Query: 244 DFLTILYQPPQINALHLMKDGNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASE 303
           DFLT+L Q  Q+  LHLMKD  W+AV+P P AL+VNIGDL Q  SN+ +KS++H+V+A+ 
Sbjct: 249 DFLTVLCQ-DQVGGLHLMKDSRWVAVRPRPDALVVNIGDLFQAWSNNRYKSVEHKVVANA 308

Query: 304 AVERFSFAYFYCPSDDVMIESCTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHN 341
             +R S AYF CPS D ++ +C +PS YR F++ EYR+++++DV  TG K+GLP F  H+
Sbjct: 309 KTDRLSVAYFLCPSYDSLVGTCGEPSPYRAFTFGEYRKKVQEDVRTTGKKIGLPNFFKHS 355

[Close]

BLAST of MC10g0998 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.1e-62
Identity = 145/341 (42.52%), Postives = 197/341 (57.77%), Query Frame = 0


Query: 6   PPLQVFYNALMKKDGEEGENGRILEDERELVVEECDELPLIDLGLLKMGNLEREK-CKRQ 65
           PPL   Y  L        E  R+  D   + V+EC ELP+IDL     G +E E+ C+  
Sbjct: 12  PPLMSTYKHLFV------EQHRLDMDMGAIDVDEC-ELPVIDLA----GLMEAEQVCRAD 71

Query: 66  IVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLS-GSYRWGNSQ 125
           +V AA  WGFFQV NHGVP+  L+ +   Q  VF +PF++K     +  S  SYRWG   
Sbjct: 72  MVRAASEWGFFQVTNHGVPQALLRELHDAQVAVFRRPFQEKVTERLLGFSPESYRWGTPT 131

Query: 126 AISPTQISWSEAFHIAVLEV-PSLEDQYLSLRSTVEGVVKKFGGLAESIAEILGQSL-GM 185
           A    Q+SWSEA+HI +    PS     +  R+ +E V +    LA+ +AEIL + L G 
Sbjct: 132 AKCLEQLSWSEAYHIPMTTPRPSTS---IRARAVIEEVSRAMYELAQKLAEILMRGLPGA 191

Query: 186 KSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQ-INALHLMKD 245
                  + +      RLNRYPPC  A   +GL PHTD+D LTI++Q    +  L L+K 
Sbjct: 192 GEGETMVTTREETCFLRLNRYPPCAMAMGGFGLCPHTDSDLLTIVHQQQDTVGGLQLLKG 251

Query: 246 GNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDDVMIE 305
           G W+AVKP+P  L+VN+GDLLQ  SNDV+KS++HRV+A+  +ERFS A+F CPS   +I 
Sbjct: 252 GRWVAVKPSPSTLIVNVGDLLQAWSNDVYKSVEHRVMANATLERFSMAFFLCPSYHTLII 311

Query: 306 SCTK-----PSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRF 337
             +       + YR F++ EYR+QI +DV  TG K+GL RF
Sbjct: 312 PSSSHVHDDDAHYRSFTFGEYRKQIMEDVRSTGRKIGLHRF 338

[Close]

BLAST of MC10g0998 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.4e-38
Identity = 98/292 (33.56%), Postives = 153/292 (52.40%), Query Frame = 0


Query: 43  LPLIDL-GLLKMGNLEREKCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQP 102
           +P+IDL GL     L  +    +I EA R WGFFQVVNHGV    + A     ++ F+ P
Sbjct: 62  IPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMP 121

Query: 103 FEKKSLRNFMNLSGSYR-WGNSQAISP-TQISWSEAFHIAVLEVPSLED--QYLSLRSTV 162
              K    + N   +Y  +G+   +     + WS+ + + +L    L+D  ++ S   T+
Sbjct: 122 VNAK--ETYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHLLP-HHLKDFNKWPSFPPTI 181

Query: 163 EGVVKKFG----GLAESIAEILGQSLGMKSSYFEESC--KRGRSSFRLNRYPPCPFASKV 222
             V+ ++G     L+  I  +L  +LG+K   F+E+   +   +  R+N YP CP     
Sbjct: 182 REVIDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELA 241

Query: 223 YGLVPHTDTDFLTILYQPPQINALHLMKDGNWLAVKPNPQALLVNIGDLLQVVSNDVFKS 282
            GL PH+D   +TIL    Q+  L + KD  W+ VKP+P A +VNIGD +Q++SN  +KS
Sbjct: 242 LGLSPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKS 301

Query: 283 LKHRVIASEAVERFSFAYFYCPSDDVMIE------SCTKPSIYRQFSYKEYR 318
           ++HRVI +   ER S A+FY P  D+ I+      S   P +Y   ++ +YR
Sbjct: 302 VEHRVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYR 350

[Close]

BLAST of MC10g0998 vs. NCBI nr
Match: XP_022158691.1 (gibberellin 2-beta-dioxygenase 8-like [Momordica charantia])

HSP 1 Score: 689 bits (1778), Expect = 5.88e-250
Identity = 341/341 (100.00%), Postives = 341/341 (100.00%), Query Frame = 0


Query: 2   MEIEPPLQVFYNALMKKDGEEGENGRILEDERELVVEECDELPLIDLGLLKMGNLEREKC 61
           MEIEPPLQVFYNALMKKDGEEGENGRILEDERELVVEECDELPLIDLGLLKMGNLEREKC
Sbjct: 1   MEIEPPLQVFYNALMKKDGEEGENGRILEDERELVVEECDELPLIDLGLLKMGNLEREKC 60

Query: 62  KRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGSYRWGN 121
           KRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGSYRWGN
Sbjct: 61  KRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGSYRWGN 120

Query: 122 SQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILGQSLGM 181
           SQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILGQSLGM
Sbjct: 121 SQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILGQSLGM 180

Query: 182 KSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALHLMKDG 241
           KSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALHLMKDG
Sbjct: 181 KSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALHLMKDG 240

Query: 242 NWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDDVMIES 301
           NWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDDVMIES
Sbjct: 241 NWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDDVMIES 300

Query: 302 CTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM 342
           CTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM
Sbjct: 301 CTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM 341

[Close]

BLAST of MC10g0998 vs. NCBI nr
Match: XP_038906005.1 (gibberellin 2-beta-dioxygenase 8-like [Benincasa hispida])

HSP 1 Score: 542 bits (1396), Expect = 7.73e-192
Identity = 271/348 (77.87%), Postives = 308/348 (88.51%), Query Frame = 0


Query: 1   SMEIEPPLQVFYNALMKKDGEEGENGRIL---EDERELVVEECD---ELPLIDLGLLKMG 60
           SMEIEPPL+V Y ALMK D  EG+NGR+    ED+ + VVEEC+   ELP+IDLGLLKMG
Sbjct: 2   SMEIEPPLEVRYYALMK-DEMEGKNGRLKAEEEDDHDEVVEECEDEIELPMIDLGLLKMG 61

Query: 61  NLEREKCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLS 120
           NLE+EKCK +IVEAARNWGFFQ++NHGVPE+ LKA+++EQKKVFNQPF  KSL NF+NL+
Sbjct: 62  NLEKEKCKNEIVEAARNWGFFQILNHGVPEKVLKAMIYEQKKVFNQPFANKSLSNFLNLA 121

Query: 121 GSYRWGNSQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEI 180
           G+YRWGN  AISPTQISWSEAFHIAVLEV SL D +L+LRST+EGVVKKFG LAE+I+E+
Sbjct: 122 GTYRWGNPVAISPTQISWSEAFHIAVLEVSSLND-HLTLRSTMEGVVKKFGRLAENISEV 181

Query: 181 LGQSLGMKSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINA 240
           LGQSLG+KSSYF+E C++G+SSFRLNRYPPCPFASKVYGL+PHTD+D+LTILYQP Q + 
Sbjct: 182 LGQSLGIKSSYFKERCEKGKSSFRLNRYPPCPFASKVYGLIPHTDSDYLTILYQP-QTSG 241

Query: 241 LHLMKDGNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPS 300
           L LMK G W  VKPNPQALLVNIGDL QVVSNDVF+SLKHRV+ASE VERFSFAYFYCPS
Sbjct: 242 LQLMKAGKWFPVKPNPQALLVNIGDLFQVVSNDVFRSLKHRVVASEGVERFSFAYFYCPS 301

Query: 301 DDVMIESCTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM 342
           DDVMIES  KPSIYRQFSYKEYRQQIEKDVEKTG+KVGL RF LHNI+
Sbjct: 302 DDVMIESWLKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLSRFFLHNIL 346

[Close]

BLAST of MC10g0998 vs. NCBI nr
Match: XP_022985245.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita maxima])

HSP 1 Score: 529 bits (1362), Expect = 1.08e-186
Identity = 264/346 (76.30%), Postives = 307/346 (88.73%), Query Frame = 0


Query: 1   SMEIEPPLQVFYNALMKKDGEEGENGR-ILEDERELVVEECDE---LPLIDLGLLKMGNL 60
           SMEIEPPLQV Y ALMK++ E  +NGR + E+E   VVEEC+E   LP+IDLGLLKMGN 
Sbjct: 2   SMEIEPPLQVRYYALMKEESE-SKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNS 61

Query: 61  EREKCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGS 120
           EREKCK ++VEAARNWGFFQV+NHGVPER LK +M+EQ+KVFNQPF KKSL N +N++G+
Sbjct: 62  EREKCKSEMVEAARNWGFFQVLNHGVPERVLKGMMYEQEKVFNQPFAKKSLSNVLNMAGT 121

Query: 121 YRWGNSQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILG 180
           YRWGN  A+SP+QISWSEAFH+AVLEV +L  ++++LR T+E VVKKFG LAESIAEILG
Sbjct: 122 YRWGNPVAMSPSQISWSEAFHMAVLEVSNL-GEHVTLRPTMEAVVKKFGRLAESIAEILG 181

Query: 181 QSLGMKSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALH 240
           QSLG+KSSYF+E C++GRSSFRLNRYPPCPFAS+VYGL+PHTD+D+LTILYQ  Q++ L 
Sbjct: 182 QSLGIKSSYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQA-QVSGLQ 241

Query: 241 LMKDGNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDD 300
           LMKDG W +VKPNP+ALLVNIGDL QVVSND F+SLKHRV+ASEAVERFSFAYFY PSDD
Sbjct: 242 LMKDGKWFSVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYSPSDD 301

Query: 301 VMIESCTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM 342
           VMIES TKPSIYRQFSYKEYRQQIEKDVEKTG+KVGLPRFLLHN++
Sbjct: 302 VMIESYTKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLPRFLLHNML 344

[Close]

BLAST of MC10g0998 vs. NCBI nr
Match: XP_022932133.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita moschata] >KAG6577443.1 Gibberellin 2-beta-dioxygenase 8, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015515.1 Gibberellin 2-beta-dioxygenase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 528 bits (1360), Expect = 2.17e-186
Identity = 263/346 (76.01%), Postives = 305/346 (88.15%), Query Frame = 0


Query: 1   SMEIEPPLQVFYNALMKKDGEEGENGR-ILEDERELVVEECDE---LPLIDLGLLKMGNL 60
           SMEIEPPL V Y ALMK++ E  +NGR + E+E   VVEEC+E   LP+IDLGLLKMGN 
Sbjct: 2   SMEIEPPLHVRYYALMKEESE-SKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNS 61

Query: 61  EREKCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGS 120
           EREKC  ++VEAARNWGFFQV+NHGVPER LK +M+EQKKVFNQPF KKSL N +NL+G+
Sbjct: 62  EREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGT 121

Query: 121 YRWGNSQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILG 180
           YRWGN  A+SP+QISWSEAFH+AVLEV +LE+ +++LR T+E VVKKFG LAESIAEILG
Sbjct: 122 YRWGNPVAMSPSQISWSEAFHMAVLEVSNLEE-HVTLRPTMEAVVKKFGRLAESIAEILG 181

Query: 181 QSLGMKSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALH 240
           QSLG+K+ YF+E C++GRSSFRLNRYPPCPFAS+VYGL+PHTD+D+LTILYQ  Q++ L 
Sbjct: 182 QSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQA-QVSGLQ 241

Query: 241 LMKDGNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDD 300
           LMKDG W AVKPNP+ALLVNIGDL QVVSND F+SLKHRV+ASEAVERFSFAYFY PSDD
Sbjct: 242 LMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYSPSDD 301

Query: 301 VMIESCTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM 342
           VMIESCTKPSIYRQFSY+EYRQQIEKDVEKTG+KVGLPRF LHN++
Sbjct: 302 VMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFFLHNML 344

[Close]

BLAST of MC10g0998 vs. NCBI nr
Match: KAG6600754.1 (Gibberellin 2-beta-dioxygenase 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 515 bits (1326), Expect = 5.39e-181
Identity = 257/342 (75.15%), Postives = 300/342 (87.72%), Query Frame = 0


Query: 1   SMEIEPPLQVFYNALMKKDGEEGENGRILEDERELVVEECDELPLIDLGLLKMGNLEREK 60
           SM+I+PPLQV YNALMK + E G++GR+ EDE   V E  ++LP+IDLGLLKMGNLEREK
Sbjct: 14  SMDIDPPLQVCYNALMKGEVE-GKSGRV-EDE---VEETYEDLPVIDLGLLKMGNLEREK 73

Query: 61  CKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGSYRWG 120
           CK++IVEAARNWGFFQV+NHGVPERALKA+++EQKKVF  PF  KSL NF+NL G+YRWG
Sbjct: 74  CKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLGNFLNLGGTYRWG 133

Query: 121 NSQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILGQSLG 180
           N  A+SP+QISWSEAFHIAVL+V SLE+ +++LRST+E V KKFG LAESIAEILGQSLG
Sbjct: 134 NPLALSPSQISWSEAFHIAVLDVSSLEE-HVTLRSTMETVAKKFGSLAESIAEILGQSLG 193

Query: 181 MKSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALHLMKD 240
           +K SYF+E C++G+SS RLNRYPPCPFASKVYGL+PHTD+D+LTILYQ    + L LM+D
Sbjct: 194 IKWSYFKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQA-HTSGLQLMRD 253

Query: 241 GNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDDVMIE 300
           G W  VKPNPQ LLVNIGDLLQVVSNDVFKS+KHRV+ASE  ERFSFAYFYCPS+DVMIE
Sbjct: 254 GTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDVMIE 313

Query: 301 SCTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM 342
           SC KPSIY+QFSYKEYRQQIEKDVEKTG+KVGL RF LH+++
Sbjct: 314 SCMKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFLHHML 348

[Close]

BLAST of MC10g0998 vs. ExPASy TrEMBL
Match: A0A6J1E1P5 (gibberellin 2-beta-dioxygenase 8-like OS=Momordica charantia OX=3673 GN=LOC111025151 PE=3 SV=1)

HSP 1 Score: 689 bits (1778), Expect = 2.85e-250
Identity = 341/341 (100.00%), Postives = 341/341 (100.00%), Query Frame = 0


Query: 2   MEIEPPLQVFYNALMKKDGEEGENGRILEDERELVVEECDELPLIDLGLLKMGNLEREKC 61
           MEIEPPLQVFYNALMKKDGEEGENGRILEDERELVVEECDELPLIDLGLLKMGNLEREKC
Sbjct: 1   MEIEPPLQVFYNALMKKDGEEGENGRILEDERELVVEECDELPLIDLGLLKMGNLEREKC 60

Query: 62  KRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGSYRWGN 121
           KRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGSYRWGN
Sbjct: 61  KRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGSYRWGN 120

Query: 122 SQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILGQSLGM 181
           SQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILGQSLGM
Sbjct: 121 SQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILGQSLGM 180

Query: 182 KSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALHLMKDG 241
           KSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALHLMKDG
Sbjct: 181 KSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALHLMKDG 240

Query: 242 NWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDDVMIES 301
           NWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDDVMIES
Sbjct: 241 NWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDDVMIES 300

Query: 302 CTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM 342
           CTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM
Sbjct: 301 CTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM 341

[Close]

BLAST of MC10g0998 vs. ExPASy TrEMBL
Match: A0A6J1JAT6 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111483296 PE=3 SV=1)

HSP 1 Score: 529 bits (1362), Expect = 5.22e-187
Identity = 264/346 (76.30%), Postives = 307/346 (88.73%), Query Frame = 0


Query: 1   SMEIEPPLQVFYNALMKKDGEEGENGR-ILEDERELVVEECDE---LPLIDLGLLKMGNL 60
           SMEIEPPLQV Y ALMK++ E  +NGR + E+E   VVEEC+E   LP+IDLGLLKMGN 
Sbjct: 2   SMEIEPPLQVRYYALMKEESE-SKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNS 61

Query: 61  EREKCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGS 120
           EREKCK ++VEAARNWGFFQV+NHGVPER LK +M+EQ+KVFNQPF KKSL N +N++G+
Sbjct: 62  EREKCKSEMVEAARNWGFFQVLNHGVPERVLKGMMYEQEKVFNQPFAKKSLSNVLNMAGT 121

Query: 121 YRWGNSQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILG 180
           YRWGN  A+SP+QISWSEAFH+AVLEV +L  ++++LR T+E VVKKFG LAESIAEILG
Sbjct: 122 YRWGNPVAMSPSQISWSEAFHMAVLEVSNL-GEHVTLRPTMEAVVKKFGRLAESIAEILG 181

Query: 181 QSLGMKSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALH 240
           QSLG+KSSYF+E C++GRSSFRLNRYPPCPFAS+VYGL+PHTD+D+LTILYQ  Q++ L 
Sbjct: 182 QSLGIKSSYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQA-QVSGLQ 241

Query: 241 LMKDGNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDD 300
           LMKDG W +VKPNP+ALLVNIGDL QVVSND F+SLKHRV+ASEAVERFSFAYFY PSDD
Sbjct: 242 LMKDGKWFSVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYSPSDD 301

Query: 301 VMIESCTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM 342
           VMIES TKPSIYRQFSYKEYRQQIEKDVEKTG+KVGLPRFLLHN++
Sbjct: 302 VMIESYTKPSIYRQFSYKEYRQQIEKDVEKTGNKVGLPRFLLHNML 344

[Close]

BLAST of MC10g0998 vs. ExPASy TrEMBL
Match: A0A6J1F0S8 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111438457 PE=3 SV=1)

HSP 1 Score: 528 bits (1360), Expect = 1.05e-186
Identity = 263/346 (76.01%), Postives = 305/346 (88.15%), Query Frame = 0


Query: 1   SMEIEPPLQVFYNALMKKDGEEGENGR-ILEDERELVVEECDE---LPLIDLGLLKMGNL 60
           SMEIEPPL V Y ALMK++ E  +NGR + E+E   VVEEC+E   LP+IDLGLLKMGN 
Sbjct: 2   SMEIEPPLHVRYYALMKEESE-SKNGRWVGEEEEREVVEECEEEEELPVIDLGLLKMGNS 61

Query: 61  EREKCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGS 120
           EREKC  ++VEAARNWGFFQV+NHGVPER LK +M+EQKKVFNQPF KKSL N +NL+G+
Sbjct: 62  EREKCISEMVEAARNWGFFQVLNHGVPERVLKGMMYEQKKVFNQPFAKKSLSNVLNLAGT 121

Query: 121 YRWGNSQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILG 180
           YRWGN  A+SP+QISWSEAFH+AVLEV +LE+ +++LR T+E VVKKFG LAESIAEILG
Sbjct: 122 YRWGNPVAMSPSQISWSEAFHMAVLEVSNLEE-HVTLRPTMEAVVKKFGRLAESIAEILG 181

Query: 181 QSLGMKSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALH 240
           QSLG+K+ YF+E C++GRSSFRLNRYPPCPFAS+VYGL+PHTD+D+LTILYQ  Q++ L 
Sbjct: 182 QSLGIKACYFKERCEKGRSSFRLNRYPPCPFASQVYGLIPHTDSDYLTILYQA-QVSGLQ 241

Query: 241 LMKDGNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDD 300
           LMKDG W AVKPNP+ALLVNIGDL QVVSND F+SLKHRV+ASEAVERFSFAYFY PSDD
Sbjct: 242 LMKDGKWFAVKPNPKALLVNIGDLFQVVSNDAFRSLKHRVVASEAVERFSFAYFYSPSDD 301

Query: 301 VMIESCTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM 342
           VMIESCTKPSIYRQFSY+EYRQQIEKDVEKTG+KVGLPRF LHN++
Sbjct: 302 VMIESCTKPSIYRQFSYREYRQQIEKDVEKTGNKVGLPRFFLHNML 344

[Close]

BLAST of MC10g0998 vs. ExPASy TrEMBL
Match: A0A6J1JF15 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111484442 PE=3 SV=1)

HSP 1 Score: 514 bits (1323), Expect = 3.37e-181
Identity = 254/341 (74.49%), Postives = 299/341 (87.68%), Query Frame = 0


Query: 2   MEIEPPLQVFYNALMKKDGEEGENGRILEDERELVVEECDELPLIDLGLLKMGNLEREKC 61
           M+I+PPLQV YNALMK D  EG+NGR+ +DE   V E  ++LP+IDLGLLKMGNLEREKC
Sbjct: 1   MDIDPPLQVCYNALMK-DEVEGKNGRV-DDE---VEETYEDLPVIDLGLLKMGNLEREKC 60

Query: 62  KRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGSYRWGN 121
           K++IVEAARNWGFFQV+NHGVPERALKA+++EQKKVF  PF +KSL NF+NL G+YRWGN
Sbjct: 61  KKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAQKSLGNFLNLGGTYRWGN 120

Query: 122 SQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILGQSLGM 181
             A+SP+Q+SWSEAFHI VL+V SLE+ +++LRST+E V KKFG +AESIAEILGQSLG+
Sbjct: 121 PLALSPSQMSWSEAFHIGVLDVSSLEE-HVTLRSTMETVAKKFGSIAESIAEILGQSLGI 180

Query: 182 KSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALHLMKDG 241
           K SYF+E C++G+SS RLNRYPPCPFASKVYGL+PHTD+D+LTILYQ    + L LM+DG
Sbjct: 181 KWSYFKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQA-HTSGLQLMRDG 240

Query: 242 NWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDDVMIES 301
            W  VKPNPQ LLVNIGDLLQVVSNDVFKS+KHRV+ASE  ERFSFAYFYCPS+DVMIES
Sbjct: 241 TWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDVMIES 300

Query: 302 CTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM 342
           C KPSIY+QFSYKEYRQQIEKDVEKTG+KVGL RF LH+++
Sbjct: 301 CMKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFLHHVL 334

[Close]

BLAST of MC10g0998 vs. ExPASy TrEMBL
Match: A0A6J1FQ56 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447223 PE=3 SV=1)

HSP 1 Score: 513 bits (1322), Expect = 7.95e-181
Identity = 257/342 (75.15%), Postives = 298/342 (87.13%), Query Frame = 0


Query: 1   SMEIEPPLQVFYNALMKKDGEEGENGRILEDERELVVEECDELPLIDLGLLKMGNLEREK 60
           SM+I+PPLQV Y ALMK D  EG++GR+ EDE   V E  ++LP+IDLGLLKMGNLEREK
Sbjct: 14  SMDIDPPLQVCYKALMK-DEVEGKSGRV-EDE---VEETYEDLPVIDLGLLKMGNLEREK 73

Query: 61  CKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGSYRWG 120
           CK++IVEAARNWGFFQV+NHGVPERALKA+++EQKKVF  PF  KSL NF+NL G+YRWG
Sbjct: 74  CKKEIVEAARNWGFFQVMNHGVPERALKALLYEQKKVFYHPFAHKSLGNFLNLGGTYRWG 133

Query: 121 NSQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILGQSLG 180
           N  A+SP+QISWSEAFHIAVL+V SLE+ + +LRST+E V KKFG LAESIAEILGQSLG
Sbjct: 134 NPLALSPSQISWSEAFHIAVLDVSSLEE-HATLRSTMETVAKKFGSLAESIAEILGQSLG 193

Query: 181 MKSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALHLMKD 240
           +K SYF+E C++G+SS RLNRYPPCPFASKVYGL+PHTD+D+LTILYQ    + L LM+D
Sbjct: 194 IKWSYFKERCEKGKSSLRLNRYPPCPFASKVYGLIPHTDSDYLTILYQA-HTSGLQLMRD 253

Query: 241 GNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDDVMIE 300
           G W  VKPNPQ LLVNIGDLLQVVSNDVFKS+KHRV+ASE  ERFSFAYFYCPS+DVMIE
Sbjct: 254 GTWFPVKPNPQYLLVNIGDLLQVVSNDVFKSIKHRVVASEEDERFSFAYFYCPSEDVMIE 313

Query: 301 SCTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLHNIM 342
           SC KPSIY+QFSYKEYRQQIEKDVEKTG+KVGL RF LH+++
Sbjct: 314 SCMKPSIYKQFSYKEYRQQIEKDVEKTGNKVGLSRFFLHHML 348

[Close]

BLAST of MC10g0998 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 318.5 bits (815), Expect = 6.4e-87
Identity = 167/342 (48.83%), Postives = 225/342 (65.79%), Query Frame = 0


Query: 4   IEPPLQVFYNALM-----KKDGEEGENGRILEDERELVVEECDELPLIDLG-LLKMGNLE 63
           ++PP    YN L+     KKD +  E           VVEE  ELP+ID+  L+     E
Sbjct: 1   MDPPFNEIYNNLLYNQITKKDNDVSEIP--FSFSVTAVVEEV-ELPVIDVSRLIDGAEEE 60

Query: 64  REKCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPFEKKSLRNFMNLSGSY 123
           REKCK  I  A+R WGFFQV+NHG+    L+ +  EQ +VF +PF+KKS     + +GSY
Sbjct: 61  REKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFREPFDKKSKSEKFS-AGSY 120

Query: 124 RWGNSQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEGVVKKFGGLAESIAEILGQ 183
           RWG   A S  Q+SWSEAFH+ + ++   +D + +L ST+E    +   LA  +AE+L +
Sbjct: 121 RWGTPSATSIRQLSWSEAFHVPMTDISDNKD-FTTLSSTMEKFASESEALAYMLAEVLAE 180

Query: 184 SLGMKSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTDTDFLTILYQPPQINALHL 243
             G  SS+F+E+C R     R+NRYPPCP  S+VYGL+PHTD+DFLTILYQ  Q+  L L
Sbjct: 181 KSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGLMPHTDSDFLTILYQ-DQVGGLQL 240

Query: 244 MKDGNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIASEAVERFSFAYFYCPSDDV 303
           +KD  W+AVKPNP+AL++NIGDL Q  SN ++KS++HRV+ +  VERFS AYF CPS D 
Sbjct: 241 IKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYDA 300

Query: 304 MIESCTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLLH 340
           +IE  +    YR FS++E+RQQ+++DV+K G KVGLPRFL H
Sbjct: 301 VIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPRFLNH 336

[Close]

BLAST of MC10g0998 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 288.5 bits (737), Expect = 7.0e-78
Identity = 146/299 (48.83%), Postives = 203/299 (67.89%), Query Frame = 0


Query: 42  ELPLIDLGLLKMG-NLEREKCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQ 101
           +LP+IDL  L  G  ++R++C +Q+V AA+ WGFFQ+VNHG+P+   + ++ E+KK+F+Q
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 102 PFEKKSLRNFMNLS-GSYRWGNSQAISPTQISWSEAFHIAVLEVPSLEDQYLSLRSTVEG 161
           PF  K    F +LS  SYRWGN  A SP Q S SEAFHI + EV  + D   +LR+ VE 
Sbjct: 99  PFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDRNNLRTIVET 158

Query: 162 VVKKFGGLAESIAEILGQSLGMKSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLVPHTD 221
            V++   +A+ I EILG+ + + S YFE   +   S  RLN+Y P  F S+V+GLVPHTD
Sbjct: 159 YVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPHTD 218

Query: 222 TDFLTILYQPPQINALHLMKDGNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHRVIAS 281
           T FLTIL Q  QI  L L  +G W++VKP  +AL VNIGD+ Q +SN V++S++HRVI+ 
Sbjct: 219 TSFLTILSQ-DQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVISP 278

Query: 282 EAVERFSFAYFYCPSDDVMIESCTKPSIYRQFSYKEYRQQIEKDVEKTGDKVGLPRFLL 339
             +ER S A+F CP  +  I+    P  YR+FS++EY++Q E DV++TGDKVGL RFL+
Sbjct: 279 ANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRFLI 336

[Close]

BLAST of MC10g0998 vs. TAIR 10
Match: AT5G05600.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 161.0 bits (406), Expect = 1.7e-39
Identity = 98/292 (33.56%), Postives = 153/292 (52.40%), Query Frame = 0


Query: 43  LPLIDL-GLLKMGNLEREKCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQP 102
           +P+IDL GL     L  +    +I EA R WGFFQVVNHGV    + A     ++ F+ P
Sbjct: 62  IPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMP 121

Query: 103 FEKKSLRNFMNLSGSYR-WGNSQAISP-TQISWSEAFHIAVLEVPSLED--QYLSLRSTV 162
              K    + N   +Y  +G+   +     + WS+ + + +L    L+D  ++ S   T+
Sbjct: 122 VNAK--ETYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHLLP-HHLKDFNKWPSFPPTI 181

Query: 163 EGVVKKFG----GLAESIAEILGQSLGMKSSYFEESC--KRGRSSFRLNRYPPCPFASKV 222
             V+ ++G     L+  I  +L  +LG+K   F+E+   +   +  R+N YP CP     
Sbjct: 182 REVIDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELA 241

Query: 223 YGLVPHTDTDFLTILYQPPQINALHLMKDGNWLAVKPNPQALLVNIGDLLQVVSNDVFKS 282
            GL PH+D   +TIL    Q+  L + KD  W+ VKP+P A +VNIGD +Q++SN  +KS
Sbjct: 242 LGLSPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKS 301

Query: 283 LKHRVIASEAVERFSFAYFYCPSDDVMIE------SCTKPSIYRQFSYKEYR 318
           ++HRVI +   ER S A+FY P  D+ I+      S   P +Y   ++ +YR
Sbjct: 302 VEHRVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYR 350

[Close]

BLAST of MC10g0998 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 157.1 bits (396), Expect = 2.4e-38
Identity = 90/289 (31.14%), Postives = 141/289 (48.79%), Query Frame = 0


Query: 41  DELPLIDLGLLKMGNLEREKCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQ 100
           D +PLIDL  L   N  R     Q   A  + GFFQ+ NHGVPE  +K +M   ++ F Q
Sbjct: 40  DSIPLIDLHDLHGPN--RADIINQFAHACSSCGFFQIKNHGVPEETIKKMMNAAREFFRQ 99

Query: 101 PFEKKSLRNFMNLSGSYRWGNSQAISPTQIS-WSEAFHIAVLEVPSLEDQY----LSLRS 160
              ++      +   + R   S  +S  ++S W +   +    +    +++    +S R 
Sbjct: 100 SESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPIEDFINEWPSTPISFRE 159

Query: 161 TVEGVVKKFGGLAESIAEILGQSLGMKSSYFEESCKRGRSSFRLNRYPPCPFASKVYGLV 220
                      L  ++ E + +SLG+       +  +      +N YP CP     YGL 
Sbjct: 160 VTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLP 219

Query: 221 PHTDTDFLTILYQPPQINALHLMKDGNWLAVKPNPQALLVNIGDLLQVVSNDVFKSLKHR 280
            H D + +T+L Q  +++ L + KDG W+AV P P   +VN+GD +QV+SN+ +KS+ HR
Sbjct: 220 GHKDANLITVLLQ-DEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHR 279

Query: 281 VIASEAVERFSFAYFYCPSDDVMI--------ESCTKPSIYRQFSYKEY 317
            + +  +ER S   FYCPS+D +I        E    P+IYR F+Y EY
Sbjct: 280 AVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEY 325

[Close]

BLAST of MC10g0998 vs. TAIR 10
Match: AT3G11180.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 152.1 bits (383), Expect = 7.9e-37
Identity = 96/291 (32.99%), Postives = 153/291 (52.58%), Query Frame = 0


Query: 43  LPLIDLGLLKMGNLEREKCKRQIVEAARNWGFFQVVNHGVPERALKAVMFEQKKVFNQPF 102
           +P+IDL  L  GN   E  K++I EA R WGFFQV+NHGV    + A     K  FN P 
Sbjct: 95  IPIIDLDSLFSGN---EDDKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPV 154

Query: 103 EKKSLRNFMNLSGSYR-WGNSQAISPTQI-SWSEAFHIAVLEVPSLED--QYLSLRSTVE 162
           E K +  + N   +Y  +G+   +    I  W++ +++  L + +L+D  ++ SL S + 
Sbjct: 155 EAKEV--YSNSPRTYEGYGSRLGVEKGAILDWNDYYYLHFLPL-ALKDFNKWPSLPSNIR 214

Query: 163 GVVKKFG----GLAESIAEILGQSLGMKSSYFEESC--KRGRSSFRLNRYPPCPFASKVY 222
            +  ++G     L   +  IL  +LG+++   +E+   +   +  R+N YP CP      
Sbjct: 215 EMNDEYGKELVKLGGRLMTILSSNLGLRAEQLQEAFGGEDVGACLRVNYYPKCPQPELAL 274

Query: 223 GLVPHTDTDFLTILYQPPQINALHLMKDGNWLAVKPNPQALLVNIGDLLQVVSNDVFKSL 282
           GL PH+D   +TIL    Q+  L +     W+ V P   A +VNIGD +Q++SN  +KS+
Sbjct: 275 GLSPHSDPGGMTILLPDDQVVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSV 334

Query: 283 KHRVIASEAVERFSFAYFYCPSDDVMIE------SCTKPSIYRQFSYKEYR 318
           +HRVI +   ER S A+FY P  D+ I+      + T P +Y   ++ +YR
Sbjct: 335 EHRVIVNSEKERVSLAFFYNPKSDIPIQPMQQLVTSTMPPLYPPMTFDQYR 379

The following BLAST results are available for this feature:

BLAST of MC10g0998 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49561	8.9e-86	48.83	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
Q9C6I4	9.9e-77	48.83	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ...	[more]
Q7XP65	5.6e-72	40.28	Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q8LGZ9	1.1e-62	42.52	Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q9FFF6	2.4e-38	33.56	Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1	[more]

BLAST of MC10g0998 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158691.1	5.88e-250	100.00	gibberellin 2-beta-dioxygenase 8-like [Momordica charantia]	[more]
XP_038906005.1	7.73e-192	77.87	gibberellin 2-beta-dioxygenase 8-like [Benincasa hispida]	[more]
XP_022985245.1	1.08e-186	76.30	gibberellin 2-beta-dioxygenase 8-like [Cucurbita maxima]	[more]
XP_022932133.1	2.17e-186	76.01	gibberellin 2-beta-dioxygenase 8-like [Cucurbita moschata] >KAG6577443.1 Gibbere...	[more]
KAG6600754.1	5.39e-181	75.15	Gibberellin 2-beta-dioxygenase 6, partial [Cucurbita argyrosperma subsp. sororia...	[more]

BLAST of MC10g0998 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E1P5	2.85e-250	100.00	gibberellin 2-beta-dioxygenase 8-like OS=Momordica charantia OX=3673 GN=LOC11102...	[more]
A0A6J1JAT6	5.22e-187	76.30	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC11148329...	[more]
A0A6J1F0S8	1.05e-186	76.01	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111438...	[more]
A0A6J1JF15	3.37e-181	74.49	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC11148444...	[more]
A0A6J1FQ56	7.95e-181	75.15	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447...	[more]

BLAST of MC10g0998 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21200.1	6.4e-87	48.83	gibberellin 2-oxidase 8	[more]
AT1G50960.1	7.0e-78	48.83	gibberellin 2-oxidase 7	[more]
AT5G05600.1	1.7e-39	33.56	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G10490.1	2.4e-38	31.14	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G11180.1	7.9e-37	32.99	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 40..332 e-value: 3.1E-74 score: 252.1
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 43..141 e-value: 4.4E-16 score: 59.7
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 197..293 e-value: 5.3E-21 score: 75.0
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 32..331
None	No IPR available	PANTHER	PTHR47990:SF19	20-OXIDASE, PUTATIVE-RELATED	coord: 32..331
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 39..320
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 193..293 score: 11.87214

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g0998.1	MC10g0998.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding