MC10g0946 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g0946
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	TIR domain-containing protein-like
Location	MC10: 8192206 .. 8194505 (+)
RNA-Seq Expression	MC10g0946
Synteny	MC10g0946

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATCGTGTGGGGGGCGCTGTGCAGAAGATAACGCCAAGCCAGTTTCTAGCTCAGCTGGGTAAGATGTCGCTCGCAGACCAAAGGATCATAAAGCCATGCGATGTATTCATTAACCACAGAGGCGTCGACACGAAGAAGACTCTCGCCGCCCTGCTCTACGACAGGCTTCTCCGCTTCAACCTCCGCCCCTTCCTCGATTACAGGCAGATCATTACGTTATATTCTATTTTAGTCTCTAAACTTTTAAAGTTTATGTTTTATTTTAGTCTATAATTATTTTTTTTTCCTAATGGGTAGGAACCTGAAGCCTGGAGAGAAGTTGTTTGATGAAATTCATGGGGCGATTTGGCAGTGTAAAGTTGGTGTGGCTGTGTTTTCGCCTCGTTATTGCGAGTCTTACTTTTGCCTACATGAGTTGGCTATGATCATGGAGTCTAAGAAGAAGGTGTGGCTATGGATTTTATTGGAATTCCTTTTCTTTTTGCTTATTCACATAATCGCACGTCCTCATTTTCTGAAAAGTTTTAAATGTTTATATCAAATTTATGAAAATATCGATCTGTGAATGAAATTTTTCTAAAAAATTCATAAAAAATATTTATTATTTTTAAATAAATTAAAGATTATTAATATTAATATTATGTCTATATTGGTAATATTTCGATGATTATTTTTATATTTTGTTGTTCTTTTACAAGATGTTGATGGAAATATCGAAGAAATTAATATCTTAATCAACTCACTTAAACTTCTTTTTTTTTTAATTAGGGGTCTTAGGGCATTTAACAATGATTTAATTCAGAAATTTTGAAAATTAAATATTTGTTAATTATTTTATTTTCTTTGTTTTACAATATTACTTTATAAATAAGAGGGTATTTATGTGAATGACCCCGTAAGGAAGGCACATATGTCACCTATTTTATACCTATCATTTTTTTTATAGAAGATATTTATACCTATCCTTTTCCTACGTATTTTATTTTTAAAAAAATATTGTTAAACTTTAGCAAATTTTTAAATATTTAAGAAAATAGATTAAAAAAAGCTACAAATTTTTTTTTGAGTTCTCAAAATTTGCAAATCTTAAACCTTTATTTTGAAAATAGTTATAGACATCATTGTGGACTGAGAAATCTACCGATCATACCGTAGATTATGGGTTTTTACCATCCATTTATTAGTGATTAGGGTTTTTATTATTTTTATTAAAAATACTTTTCAAGTCTTAGTCCAATTTTGAAAACTATTTTTTAATTTCCTAGAATTTCAATAACACTTTTGAAAGTAGATTATAAATCAAAGAAAAATGTTAGTATTATATAAGACTTTTGAGGAAAAAAAAAACGTCATTAAATGAACCTAAGGTTTTCCTAGAAACACATGATACAATATAAATAAATAATGTATACCAGATTTGTTTTAATAGAATAGTATTTGCAGCTTTGGAAAAAAAAGAATAATATTTCCAGACTTTACAAACTTTAATAATAGAAAATTTATCTTCAAAGTATTGTAATTTAATTTATAATTATCTCATTGAATTTTAAGATTATTCTAACACTTGTAATTGTTATAAGATGAAAAATGAAAAAAAAAAATATTTGTCAATAGAAATCTTTATAAGGCTGTTGGTAACTTCTTGTTCTTTTTTCCATTTATTTTTCACTTTTTTAATAAATAAAAATAAAATTATGTTTGGTAACTATTTTTTGTTTCTTATTTATTCTTTTTCATAAAAAAAAGAAACAAAAACTTGTTTAGTAACTATTTATTGTTTCTTAAAATTATAAATTAAAAAAGTAAAAGTTTTATAATTAAAATAATTTACATTTTTATGTTATTTTTATTTTTTGATATTTATCATAGATTTTACAAGTCAAGAATAATAGTAAAATGATGTTTTTCTTAATATATTTTTGGACCAAAAAAAATAGACATGAAAAACAAAGAAACAAAAAATTTGATTTGGTTGTTTTCAAAAAATTATATACAAGTTTGTAACCCGTTATTAAAAGTGAAAAATAAGAAACAACAAGAGTTATCAAATACTTATATTAGACTAGTAACCAAGAGAAAACAAACAAAAAAATTAATGAGATTATAATAAATATTTGATTTAAATTTTCTTTTTGATTATTCTTAAACAGGTAATTCCTATCTTTTGTGATGTCAAGCCATCTCAACTTCGCATAGAGGATACAAACGAATATGCATCAGACGAGCTAAGGCGATTCAATTGGGCTCTCGAAGAAGCTAAACAATTTGTGGGACTCAAATTTGATTCCTCAAAAGAG

mRNA sequence

ATGAATCGTGTGGGGGGCGCTGTGCAGAAGATAACGCCAAGCCAGTTTCTAGCTCAGCTGGGTAAGATGTCGCTCGCAGACCAAAGGATCATAAAGCCATGCGATGTATTCATTAACCACAGAGGCGTCGACACGAAGAAGACTCTCGCCGCCCTGCTCTACGACAGGCTTCTCCGCTTCAACCTCCGCCCCTTCCTCGATTACAGGCAGATCATTACGAACCTGAAGCCTGGAGAGAAGTTGTTTGATGAAATTCATGGGGCGATTTGGCAGTGTAAAGTTGGTGTGGCTGTGTTTTCGCCTCGTTATTGCGAGTCTTACTTTTGCCTACATGAGTTGGCTATGATCATGGAGTCTAAGAAGAAGGTAATTCCTATCTTTTGTGATGTCAAGCCATCTCAACTTCGCATAGAGGATACAAACGAATATGCATCAGACGAGCTAAGGCGATTCAATTGGGCTCTCGAAGAAGCTAAACAATTTGTGGGACTCAAATTTGATTCCTCAAAAGAG

Coding sequence (CDS)

ATGAATCGTGTGGGGGGCGCTGTGCAGAAGATAACGCCAAGCCAGTTTCTAGCTCAGCTGGGTAAGATGTCGCTCGCAGACCAAAGGATCATAAAGCCATGCGATGTATTCATTAACCACAGAGGCGTCGACACGAAGAAGACTCTCGCCGCCCTGCTCTACGACAGGCTTCTCCGCTTCAACCTCCGCCCCTTCCTCGATTACAGGCAGATCATTACGAACCTGAAGCCTGGAGAGAAGTTGTTTGATGAAATTCATGGGGCGATTTGGCAGTGTAAAGTTGGTGTGGCTGTGTTTTCGCCTCGTTATTGCGAGTCTTACTTTTGCCTACATGAGTTGGCTATGATCATGGAGTCTAAGAAGAAGGTAATTCCTATCTTTTGTGATGTCAAGCCATCTCAACTTCGCATAGAGGATACAAACGAATATGCATCAGACGAGCTAAGGCGATTCAATTGGGCTCTCGAAGAAGCTAAACAATTTGTGGGACTCAAATTTGATTCCTCAAAAGAG

Protein sequence

MNRVGGAVQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLRFNLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMESKKKVIPIFCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKE

Homology

[Close]

BLAST of MC10g0946 vs. ExPASy Swiss-Prot
Match: I1GTC2 (TIR-only protein OS=Brachypodium distachyon OX=15368 GN=BRADI_1g24377v3 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 4.0e-34
Identity = 72/139 (51.80%), Postives = 98/139 (70.50%), Query Frame = 0


Query: 35  DVFINHRGVDTKKTLAALLYDRLLRFNLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKV 94
           +VFINHRGVDTK+T+A LLYDRL +  LR FLD      +++PG++L + I  AI +C V
Sbjct: 54  EVFINHRGVDTKRTVARLLYDRLAQAGLRGFLDN----MSMRPGDRLEERIGSAIRECTV 113

Query: 95  GVAVFSPRYCESYFCLHELAMIMESKKKVIPIFCDVKPSQL----RIEDTNEYASDELRR 154
            VA+FSP YC+S +CL ELAM++ES+K +IPIF D+KPS L     + D+  Y   +L R
Sbjct: 114 AVAIFSPSYCDSEYCLRELAMLVESRKAIIPIFYDIKPSDLLLPQAVADSEVYLPRDLER 173

Query: 155 FNWALEEAKQFVGLKFDSS 170
           F +AL EAK  VG+ +DS+
Sbjct: 174 FKFALREAKHTVGITYDSA 188

[Close]

BLAST of MC10g0946 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.3e-13
Identity = 43/114 (37.72%), Postives = 65/114 (57.02%), Query Frame = 0


Query: 35  DVFINHRGVDTKKTLAALLYDRLLRFNLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKV 94
           DVF++ RG DT+KT    L++ L+   +  F+D ++    LK G+ +  E+  AI + + 
Sbjct: 13  DVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKE----LKRGKSISSELMKAIGESRF 72

Query: 95  GVAVFSPRYCESYFCLHELAMIMESKKK----VIPIFCDVKPSQLRIEDTNEYA 145
            V VFS  Y  S +CL EL  I+E  +K    V+P+F DV PS +R +   EYA
Sbjct: 73  AVVVFSKNYASSTWCLEELVKILEIHEKFELIVVPVFYDVDPSTVR-KQNGEYA 121

[Close]

BLAST of MC10g0946 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 9.5e-12
Identity = 51/145 (35.17%), Postives = 74/145 (51.03%), Query Frame = 0


Query: 35  DVFINHRGVDTKKTLAALLYDRLLRFNLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKV 94
           DVF++ RG DT+KT  + LY+ L    ++ F D ++    L+ G  +  E+  AI + + 
Sbjct: 13  DVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKR----LEYGATIPGELCKAIEESQF 72

Query: 95  GVAVFSPRYCESYFCLHELAMIMESKKK----VIPIFCDVKPSQLR----------IEDT 154
            + VFS  Y  S +CL+EL  IME K +    VIPIF DV PS +R           E  
Sbjct: 73  AIVVFSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVRNQKESFAKAFEEHE 132

Query: 155 NEYASD--ELRRFNWALEEAKQFVG 164
            +Y  D   ++R+  AL EA    G
Sbjct: 133 TKYKDDVEGIQRWRIALNEAANLKG 153

[Close]

BLAST of MC10g0946 vs. ExPASy Swiss-Prot
Match: Q9SSN3 (Toll/interleukin-1 receptor-like protein OS=Arabidopsis thaliana OX=3702 GN=TIR PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.1e-10
Identity = 38/106 (35.85%), Postives = 59/106 (55.66%), Query Frame = 0


Query: 35  DVFINHRGVDTKKTLAALLYDRLLRFNLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKV 94
           DVF++ RG DT+    + LY  L+R ++R F D ++    L+ G++   E+   I   + 
Sbjct: 10  DVFLSFRGHDTRHNFISFLYKELVRRSIRTFKDDKE----LENGQRFSPELKSPIEVSRF 69

Query: 95  GVAVFSPRYCESYFCLHELAMIMESKKK----VIPIFCDVKPSQLR 137
            V V S  Y  S +CL EL  IM+ +KK    V+PIF  V+P+ +R
Sbjct: 70  AVVVVSENYAASSWCLDELVTIMDFEKKGSITVMPIFYGVEPNHVR 111

[Close]

BLAST of MC10g0946 vs. ExPASy Swiss-Prot
Match: Q9FHF0 (Disease resistance protein RPS4B OS=Arabidopsis thaliana OX=3702 GN=RPS4B PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 4.4e-09
Identity = 40/105 (38.10%), Postives = 57/105 (54.29%), Query Frame = 0


Query: 36  VFINHRGVDTKKTLAALLYDRLLRFNLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKVG 95
           VFIN RG D +    + L + L   N++ F+D        +P E L  +IH +    K+ 
Sbjct: 16  VFINFRGEDLRLGFVSHLVEALENDNIKVFID--NYADKGEPLETLLTKIHDS----KIA 75

Query: 96  VAVFSPRYCESYFCLHELAMIMESKKK----VIPIFCDVKPSQLR 137
           +A+FS +Y ES +CL ELAMI +  +K     IPIF  V PS +R
Sbjct: 76  LAIFSGKYTESTWCLRELAMIKDCVEKGKLVAIPIFYKVDPSTVR 114

[Close]

BLAST of MC10g0946 vs. NCBI nr
Match: XP_022142877.1 (disease resistance protein LAZ5 [Momordica charantia])

HSP 1 Score: 338 bits (867), Expect = 9.16e-117
Identity = 167/171 (97.66%), Postives = 167/171 (97.66%), Query Frame = 0


Query: 1   MNRVGGAVQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLRF 60
           MNRVGGAVQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLRF
Sbjct: 1   MNRVGGAVQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLRF 60

Query: 61  NLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMESK 120
           NLRPFLDYR    NLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMESK
Sbjct: 61  NLRPFLDYR----NLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMESK 120

Query: 121 KKVIPIFCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKE 171
           KKVIPIFCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKE
Sbjct: 121 KKVIPIFCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKE 167

[Close]

BLAST of MC10g0946 vs. NCBI nr
Match: XP_038904251.1 (TIR-only protein-like [Benincasa hispida])

HSP 1 Score: 268 bits (686), Expect = 1.72e-89
Identity = 136/172 (79.07%), Postives = 148/172 (86.05%), Query Frame = 0


Query: 1   MNRVGGA-VQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLR 60
           MNRV GA V  ITP QFLA LGK  +    I+KPCDVFINHRGVDTKKT+AALLYDRL+R
Sbjct: 1   MNRVVGAGVANITPRQFLAHLGKKEMKSI-IVKPCDVFINHRGVDTKKTVAALLYDRLVR 60

Query: 61  FNLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMES 120
            NLRPFLDY+    NLKPGEKLFDEIHGAI QCKVGVAVFSPRYCESYFCLHELAMIMES
Sbjct: 61  VNLRPFLDYK----NLKPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIMES 120

Query: 121 KKKVIPIFCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKE 171
            KKVIPI+CD+K SQLRIED ++Y  DEL+RFNWALEEAK+F GLKF+SSKE
Sbjct: 121 NKKVIPIYCDIKASQLRIEDNHDYEIDELKRFNWALEEAKRFDGLKFNSSKE 167

[Close]

BLAST of MC10g0946 vs. NCBI nr
Match: KAG7027684.1 (Toll/interleukin-1 receptor-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 263 bits (673), Expect = 2.83e-87
Identity = 131/171 (76.61%), Postives = 146/171 (85.38%), Query Frame = 0


Query: 1   MNRVGGAVQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLRF 60
           MNR+  AV  ITP QFLA LG   +    I+KPCDVFINHRGVDTKKT+AALLYDRL+R 
Sbjct: 2   MNRMS-AVANITPRQFLAHLGNNHMP---IVKPCDVFINHRGVDTKKTVAALLYDRLVRM 61

Query: 61  NLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMESK 120
           NLRPFLDY+    NLKPGEKLFDEIHGAI  CKVGVAVFSPRYCESYFCLHELAMI+ES 
Sbjct: 62  NLRPFLDYK----NLKPGEKLFDEIHGAIRHCKVGVAVFSPRYCESYFCLHELAMIIESN 121

Query: 121 KKVIPIFCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKE 171
           KK+IPIFCD+KPS+LRIED+N+Y  DELRRFNWAL++AKQF GLKF+S KE
Sbjct: 122 KKLIPIFCDIKPSELRIEDSNDYEIDELRRFNWALQQAKQFEGLKFNSFKE 164

[Close]

BLAST of MC10g0946 vs. NCBI nr
Match: XP_022984910.1 (TIR domain-containing protein-like [Cucurbita maxima])

HSP 1 Score: 263 bits (671), Expect = 5.51e-87
Identity = 131/171 (76.61%), Postives = 145/171 (84.80%), Query Frame = 0


Query: 1   MNRVGGAVQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLRF 60
           MNR+  AV  ITP QFLA LG   +    I+KPCDVFINHRGVDTKKT+AALLYDRL+R 
Sbjct: 1   MNRMS-AVANITPRQFLAHLGNNHMP---IVKPCDVFINHRGVDTKKTVAALLYDRLVRM 60

Query: 61  NLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMESK 120
           NLRPFLDY+    NLKPGEKLFDEIHGAI  CKVGVAVFSPRYCESYFCLHELAMI+ES 
Sbjct: 61  NLRPFLDYK----NLKPGEKLFDEIHGAIRHCKVGVAVFSPRYCESYFCLHELAMIIESN 120

Query: 121 KKVIPIFCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKE 171
           KK+IPIFCD+KPS+LRIED+N+Y  DE+RRFNWAL EAKQF GLKF+S KE
Sbjct: 121 KKLIPIFCDIKPSELRIEDSNDYEIDEVRRFNWALHEAKQFEGLKFNSFKE 163

[Close]

BLAST of MC10g0946 vs. NCBI nr
Match: KAA0060682.1 (vesicle-associated protein 1-4 [Cucumis melo var. makuwa])

HSP 1 Score: 261 bits (667), Expect = 1.35e-86
Identity = 131/172 (76.16%), Postives = 145/172 (84.30%), Query Frame = 0


Query: 1   MNRVG-GAVQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLR 60
           MNRV  G V  ITP QFLA +GK  +    I+KPCDVFINHRGVDTKKT+AALLYDRL+R
Sbjct: 1   MNRVAVGGVGNITPRQFLAHMGKKEMKGM-ILKPCDVFINHRGVDTKKTVAALLYDRLVR 60

Query: 61  FNLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMES 120
             LRPFLDY+    NL+PGEKLFDEIHGAI QCKVGVAVFSPRYCESYFCLHELAMI+ES
Sbjct: 61  VRLRPFLDYK----NLRPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIES 120

Query: 121 KKKVIPIFCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKE 171
           KKKVIPIFCD+K SQLRIED + Y +DE+RRFNWAL++AK F GLKFDSS E
Sbjct: 121 KKKVIPIFCDIKASQLRIEDHHGYETDEVRRFNWALQQAKSFDGLKFDSSTE 167

[Close]

BLAST of MC10g0946 vs. ExPASy TrEMBL
Match: A0A6J1CP46 (disease resistance protein LAZ5 OS=Momordica charantia OX=3673 GN=LOC111012886 PE=4 SV=1)

HSP 1 Score: 338 bits (867), Expect = 4.43e-117
Identity = 167/171 (97.66%), Postives = 167/171 (97.66%), Query Frame = 0


Query: 1   MNRVGGAVQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLRF 60
           MNRVGGAVQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLRF
Sbjct: 1   MNRVGGAVQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLRF 60

Query: 61  NLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMESK 120
           NLRPFLDYR    NLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMESK
Sbjct: 61  NLRPFLDYR----NLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMESK 120

Query: 121 KKVIPIFCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKE 171
           KKVIPIFCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKE
Sbjct: 121 KKVIPIFCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKE 167

[Close]

BLAST of MC10g0946 vs. ExPASy TrEMBL
Match: A0A6J1J9V9 (TIR domain-containing protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483045 PE=4 SV=1)

HSP 1 Score: 263 bits (671), Expect = 2.67e-87
Identity = 131/171 (76.61%), Postives = 145/171 (84.80%), Query Frame = 0


Query: 1   MNRVGGAVQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLRF 60
           MNR+  AV  ITP QFLA LG   +    I+KPCDVFINHRGVDTKKT+AALLYDRL+R 
Sbjct: 1   MNRMS-AVANITPRQFLAHLGNNHMP---IVKPCDVFINHRGVDTKKTVAALLYDRLVRM 60

Query: 61  NLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMESK 120
           NLRPFLDY+    NLKPGEKLFDEIHGAI  CKVGVAVFSPRYCESYFCLHELAMI+ES 
Sbjct: 61  NLRPFLDYK----NLKPGEKLFDEIHGAIRHCKVGVAVFSPRYCESYFCLHELAMIIESN 120

Query: 121 KKVIPIFCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKE 171
           KK+IPIFCD+KPS+LRIED+N+Y  DE+RRFNWAL EAKQF GLKF+S KE
Sbjct: 121 KKLIPIFCDIKPSELRIEDSNDYEIDEVRRFNWALHEAKQFEGLKFNSFKE 163

[Close]

BLAST of MC10g0946 vs. ExPASy TrEMBL
Match: A0A5A7UXT6 (Vesicle-associated protein 1-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold22G005810 PE=4 SV=1)

HSP 1 Score: 261 bits (667), Expect = 6.52e-87
Identity = 131/172 (76.16%), Postives = 145/172 (84.30%), Query Frame = 0


Query: 1   MNRVG-GAVQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLR 60
           MNRV  G V  ITP QFLA +GK  +    I+KPCDVFINHRGVDTKKT+AALLYDRL+R
Sbjct: 1   MNRVAVGGVGNITPRQFLAHMGKKEMKGM-ILKPCDVFINHRGVDTKKTVAALLYDRLVR 60

Query: 61  FNLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMES 120
             LRPFLDY+    NL+PGEKLFDEIHGAI QCKVGVAVFSPRYCESYFCLHELAMI+ES
Sbjct: 61  VRLRPFLDYK----NLRPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIES 120

Query: 121 KKKVIPIFCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKE 171
           KKKVIPIFCD+K SQLRIED + Y +DE+RRFNWAL++AK F GLKFDSS E
Sbjct: 121 KKKVIPIFCDIKASQLRIEDHHGYETDEVRRFNWALQQAKSFDGLKFDSSTE 167

[Close]

BLAST of MC10g0946 vs. ExPASy TrEMBL
Match: A0A5D3BWV6 (Vesicle-associated protein 1-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold298G001590 PE=4 SV=1)

HSP 1 Score: 259 bits (662), Expect = 3.77e-86
Identity = 130/172 (75.58%), Postives = 144/172 (83.72%), Query Frame = 0


Query: 1   MNRVG-GAVQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLR 60
           MNRV  G V  ITP QFLA +GK  +    I+KPCDVFINHRGVDTKKT+AALLYDRL+R
Sbjct: 1   MNRVAVGGVGNITPRQFLAHMGKKEMKGM-ILKPCDVFINHRGVDTKKTVAALLYDRLVR 60

Query: 61  FNLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMES 120
             LRPFLDY+    NL+PGEKLFDEIHGAI QCKVGVAVFSPRYCESYFCLHELAMI+ES
Sbjct: 61  VRLRPFLDYK----NLRPGEKLFDEIHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIES 120

Query: 121 KKKVIPIFCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKE 171
            KKVIPIFCD+K SQLRIED + Y +DE+RRFNWAL++AK F GLKFDSS E
Sbjct: 121 NKKVIPIFCDIKASQLRIEDHHGYETDEVRRFNWALQQAKSFDGLKFDSSTE 167

[Close]

BLAST of MC10g0946 vs. ExPASy TrEMBL
Match: A0A6J1ERQ8 (TIR domain-containing protein-like OS=Cucurbita moschata OX=3662 GN=LOC111435930 PE=4 SV=1)

HSP 1 Score: 257 bits (657), Expect = 3.27e-85
Identity = 127/165 (76.97%), Postives = 140/165 (84.85%), Query Frame = 0


Query: 7   AVQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLRFNLRPFL 66
           AV  ITP QFLA LG   +    I+KPCDVFINHRGVDTKKT+AALLYDRL+R NLRPFL
Sbjct: 3   AVANITPRQFLAHLGNNHMP---IVKPCDVFINHRGVDTKKTVAALLYDRLVRMNLRPFL 62

Query: 67  DYRQIITNLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMESKKKVIPI 126
           DY+    NLKPGEKLFDEIHGAI  CKVGVAVFSPRYCESYFCLHELAMI+ES KK+IPI
Sbjct: 63  DYK----NLKPGEKLFDEIHGAIRHCKVGVAVFSPRYCESYFCLHELAMIIESNKKLIPI 122

Query: 127 FCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKE 171
           FCD+KPS+LRIED N+Y  DELRRFNWAL++AKQF  LKF+S KE
Sbjct: 123 FCDIKPSELRIEDDNDYEIDELRRFNWALQQAKQFECLKFNSFKE 160

[Close]

BLAST of MC10g0946 vs. TAIR 10
Match: AT1G52900.1 (Toll-Interleukin-Resistance (TIR) domain family protein )

HSP 1 Score: 181.0 bits (458), Expect = 7.9e-46
Identity = 93/156 (59.62%), Postives = 112/156 (71.79%), Query Frame = 0


Query: 16  FLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLRFNLRPFLDYRQIITNL 75
           FL  L   S A  +I+   DVFINHRG DTK+ +A LLYD L   NLRPFLD +    N+
Sbjct: 22  FLDLLSSSSSAKPKILH--DVFINHRGSDTKRNIATLLYDNLKSRNLRPFLDSK----NM 81

Query: 76  KPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMESKKKVIPIFCDVKPSQL 135
           KPG+KLFD I+ AI   KV V VFSP YC+SYFCLHELA+IMES+K+VIPIFCD+KPSQL
Sbjct: 82  KPGDKLFDHINNAILTSKVAVTVFSPNYCDSYFCLHELALIMESRKRVIPIFCDIKPSQL 141

Query: 136 RIE-DTNEYASDELRRFNWALEEAKQFVGLKFDSSK 171
            +  +    + DE++RF WAL+EAK  VGL FDS K
Sbjct: 142 DVMIERVTCSDDEIQRFRWALQEAKDIVGLTFDSYK 171

[Close]

BLAST of MC10g0946 vs. TAIR 10
Match: AT1G61105.1 (Toll-Interleukin-Resistance (TIR) domain family protein )

HSP 1 Score: 176.0 bits (445), Expect = 2.5e-44
Identity = 84/137 (61.31%), Postives = 106/137 (77.37%), Query Frame = 0


Query: 33  PCDVFINHRGVDTKKTLAALLYDRLLRFNLRPFLDYRQIITNLKPGEKLFDEIHGAIWQC 92
           PCD+FINHRG+DTKKT++ LLYD   R  L  FLD +    +LKPG++LF EI  AI  C
Sbjct: 28  PCDIFINHRGIDTKKTISGLLYDCFTRQRLTAFLDSK----SLKPGDRLFVEIDMAIKAC 87

Query: 93  KVGVAVFSPRYCESYFCLHELAMIMESKKKVIPIFCDVKPSQLRIEDTNEYASDELRRFN 152
            VG+AVFSPRYC+SYFCLHELA+++++KK+VIPIFCDVKPS+L ++D     + E+RRF 
Sbjct: 88  GVGIAVFSPRYCDSYFCLHELALLVKNKKRVIPIFCDVKPSELCVKDDKTRPAAEIRRFQ 147

Query: 153 WALEEAKQFVGLKFDSS 170
            ALEEAK  VGL FD+S
Sbjct: 148 LALEEAKYTVGLTFDTS 160

[Close]

BLAST of MC10g0946 vs. TAIR 10
Match: AT1G17615.1 (Disease resistance protein (TIR-NBS class) )

HSP 1 Score: 75.1 bits (183), Expect = 6.1e-14
Identity = 39/106 (36.79%), Postives = 61/106 (57.55%), Query Frame = 0


Query: 35  DVFINHRGVDTKKTLAALLYDRLLRFNLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKV 94
           DVF++ RG DT++T  + LY  L+   +R F D       L+ G ++  ++  AI   K+
Sbjct: 16  DVFLSFRGKDTRRTFISFLYKELIEMGIRTFKD----DVELQSGRRIASDLLAAIENSKI 75

Query: 95  GVAVFSPRYCESYFCLHELAMIMESKKK----VIPIFCDVKPSQLR 137
            V + S  Y  S +CL EL MIM+ +KK    V+PIF +V+P+ +R
Sbjct: 76  AVVIISKNYSASPWCLQELVMIMDVEKKGSIIVMPIFYNVEPAHVR 117

[Close]

BLAST of MC10g0946 vs. TAIR 10
Match: AT1G72950.1 (Disease resistance protein (TIR-NBS class) )

HSP 1 Score: 70.5 bits (171), Expect = 1.5e-12
Identity = 40/106 (37.74%), Postives = 60/106 (56.60%), Query Frame = 0


Query: 35  DVFINHRGVDTKKTLAALLYDRLLRFNLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKV 94
           DVF++ RG+DT+++  + LY  L+R N+R F D ++    LK G ++  E+  AI   + 
Sbjct: 12  DVFLSFRGLDTRRSFISFLYKELIRRNIRTFKDDKE----LKNGRRITPELIRAIEGSRF 71

Query: 95  GVAVFSPRYCESYFCLHELAMIMESKK----KVIPIFCDVKPSQLR 137
            V V S  Y  S +CL EL  IM+ +     KV+PIF  V P  +R
Sbjct: 72  AVVVVSVNYAASRWCLEELVKIMDFENMGSLKVMPIFYGVDPCHVR 113

[Close]

BLAST of MC10g0946 vs. TAIR 10
Match: AT1G72920.1 (Toll-Interleukin-Resistance (TIR) domain family protein )

HSP 1 Score: 69.7 bits (169), Expect = 2.6e-12
Identity = 40/106 (37.74%), Postives = 59/106 (55.66%), Query Frame = 0


Query: 35  DVFINHRGVDTKKTLAALLYDRLLRFNLRPFLDYRQIITNLKPGEKLFDEIHGAIWQCKV 94
           DVF++ RG+DT++   + LY  L+R  +R F D ++    L+ G ++  E+  AI + K 
Sbjct: 10  DVFLSFRGLDTRRNFISFLYKELVRRKIRTFKDDKE----LENGRRISPELKRAIEESKF 69

Query: 95  GVAVFSPRYCESYFCLHELAMIMESKKK----VIPIFCDVKPSQLR 137
            V V S  Y  S +CL EL  IM+ + K    V+PIF  V P  LR
Sbjct: 70  AVVVVSVNYAASPWCLDELVKIMDFENKGSITVMPIFYGVDPCHLR 111

The following BLAST results are available for this feature:

BLAST of MC10g0946 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
I1GTC2	4.0e-34	51.80	TIR-only protein OS=Brachypodium distachyon OX=15368 GN=BRADI_1g24377v3 PE=1 SV=...	[more]
A0A290U7C4	2.3e-13	37.72	Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV...	[more]
Q40392	9.5e-12	35.17	TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1	[more]
Q9SSN3	3.1e-10	35.85	Toll/interleukin-1 receptor-like protein OS=Arabidopsis thaliana OX=3702 GN=TIR ...	[more]
Q9FHF0	4.4e-09	38.10	Disease resistance protein RPS4B OS=Arabidopsis thaliana OX=3702 GN=RPS4B PE=1 S...	[more]

BLAST of MC10g0946 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142877.1	9.16e-117	97.66	disease resistance protein LAZ5 [Momordica charantia]	[more]
XP_038904251.1	1.72e-89	79.07	TIR-only protein-like [Benincasa hispida]	[more]
KAG7027684.1	2.83e-87	76.61	Toll/interleukin-1 receptor-like protein, partial [Cucurbita argyrosperma subsp....	[more]
XP_022984910.1	5.51e-87	76.61	TIR domain-containing protein-like [Cucurbita maxima]	[more]
KAA0060682.1	1.35e-86	76.16	vesicle-associated protein 1-4 [Cucumis melo var. makuwa]	[more]

BLAST of MC10g0946 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CP46	4.43e-117	97.66	disease resistance protein LAZ5 OS=Momordica charantia OX=3673 GN=LOC111012886 P...	[more]
A0A6J1J9V9	2.67e-87	76.61	TIR domain-containing protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483045 P...	[more]
A0A5A7UXT6	6.52e-87	76.16	Vesicle-associated protein 1-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A5D3BWV6	3.77e-86	75.58	Vesicle-associated protein 1-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A6J1ERQ8	3.27e-85	76.97	TIR domain-containing protein-like OS=Cucurbita moschata OX=3662 GN=LOC111435930...	[more]

BLAST of MC10g0946 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52900.1	7.9e-46	59.62	Toll-Interleukin-Resistance (TIR) domain family protein	[more]
AT1G61105.1	2.5e-44	61.31	Toll-Interleukin-Resistance (TIR) domain family protein	[more]
AT1G17615.1	6.1e-14	36.79	Disease resistance protein (TIR-NBS class)	[more]
AT1G72950.1	1.5e-12	37.74	Disease resistance protein (TIR-NBS class)	[more]
AT1G72920.1	2.6e-12	37.74	Toll-Interleukin-Resistance (TIR) domain family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	SMART	SM00255	till_3	coord: 33..170 e-value: 2.6E-18 score: 76.8
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PFAM	PF01582	TIR	coord: 35..136 e-value: 1.7E-18 score: 67.0
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PROSITE	PS50104	TIR	coord: 32..171 score: 22.734278
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	GENE3D	3.40.50.10140		coord: 31..171 e-value: 1.1E-28 score: 102.1
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	SUPERFAMILY	52200	Toll/Interleukin receptor TIR domain	coord: 33..150
None	No IPR available	PANTHER	PTHR31008	COP1-INTERACTING PROTEIN-RELATED	coord: 29..170
None	No IPR available	PANTHER	PTHR31008:SF16	TOLL-INTERLEUKIN-RESISTANCE (TIR) DOMAIN FAMILY PROTEIN	coord: 29..170

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g0946.1	MC10g0946.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007165	signal transduction
molecular_function	GO:0003953	NAD+ nucleosidase activity