MC10g0912 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0912
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionHomogentisate 1,2-dioxygenase
LocationMC10: 7813923 .. 7818043 (-)
RNA-Seq ExpressionMC10g0912
SyntenyMC10g0912
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCAAAAAAAAAAAGAAATTAAAACTTTAATTGGGGGATTAGAGGATAATTAAACTGTTTCCCAATTTCGTCAAAATGTCACCATCAAGTTCTGAGTTCATCTCTATTTTTCTTTACGGCGATGGTGAGCTCCCCAAACGCTATATGACGTACTGGTTTTGATCGTGAATTCAGTTACGTGGGGAAACTTTCCGACGACCGGGACCGCCGCCAGTTCTCCGGCGCCGCCAAATCTCTGCAATCGATCTTCGTCGTCATCTCCAATGGCTGCTCAATCGGTCAACGAATCGGACGGCAGAGATTTCCCTTCCGACCTCGCCTACCAGTCCGGCTTCAACAATCATTTCTCGTCGGAGGCGATTCCCGGCGCTCTTCCTGAATCCCAAAATAGTCCTCTCATCTGCCCCTACGGCCTCTATGCTGAGCAGATCTCCGGCACTTCATTCACCTCGCCTCGAAAAGCAAACTTGCGCAGGTTCTCCTCCTTCATCTTCTCGCGTAATCGGTATGGATTTTTCTAGTTTCGTCGTCTTTGTTGTGTGAGCATTTCTGTCTTGTTGCGCAGTTGGCTGTATCGGATAAAGCCCTCGGTCACGCATGAACCGTTTAAGCCACGCTTGCCTAAGAACCAGAAGTTGATCAGTGAATTTAATGATTCGAGTTGTTCCTCGACTCCGACTCAGCTCAGGTGGAAACCGGCGGATTTTCCTGATTCGCCGGTAGATTTCGTTGATGGACTGTACACTGTCTGTGGAGCCGGCAGTTCGTTTCTTCGGCATGGGTTTGCTGTTCACATGTAATTCAGTATTCCTTTTACCTTTTCACTGCCTTTTTAGCTTGGTACTTTTTCCGACAATCAGTAAATTTTGTGGGGGAAGTTTTGTTTGTCAAATTTCTTGGTGGGGTAATTTATTTTGTCAGATTAGATGTGATGTGCCTGAGAGTTTGACTCTCTTCAATTTCAACGGGGTTTGTTCCTTACTAACAGAAAACTTAAAACACCCAAAAAATGATATTCTGGATACTATTTTTCAGCATTAGTTTGGGAAGAAAGCAAATAAAGATGATTTTTACACTGGTACTTGGTTGAATGCTATGACTGAAACAGGATTGACCATTTCCATAGGTACACAGCCAATAAGTCGATGGAGAACTGTGCGTTCTGTAATGCTGACGGCGACTTCTTGATAGTCCCTCAGAGTGGAAGTGAGTTACTACTGCAAATTATTTTTCATTGAAAATTATGAACATCGTGGTCTTGGGGTTGAAGCTGATTCTATTATTATTATTTTAGATTTGGAAAATATGGTTCCTTTTAGGTTCATAGTATGTACATTGAGTGCGTTATGTAAAGCTGATTCCATTTGGTGAAAGAGATGTTTGAGGGTAATTGGTTGATCCTTTTTATAGTTCAACTTTTGGCGACATAATCGTAGAACTGTCTTTGCATTATCTTGTCATTGAGCTCTGAAAGTCTCTAATCCGAACGGAATTATGTTATTGTTTTATAAGATATCAGGATTAATCTCTTAGAGTACTCTTATCCTGTAGGGCTGTGGATTATTACAGAGTGTGGAAGGCTGGAAGTTTCTCCAGGTGAAATAGTGGTTTTACCTCAAGGTTTTCGCTTTGTTGTTCATCTACCCGATGGGCCATCACGTGGCTACATAGCTGAGATTTTTGGTAGTCATTTTCAGCTTCCTGATCTTGGACCAATAGGTATCTAACCTCTTGTTATCTTCTTGACAGCAAGTAATTAAGGTTTTGACCATATTCCTTTCTTCCTTTTTTCCCCAAACGTAGGAAGTGGTTGTTCAGTTTGCATTTTCACCTAATGTCAGCATTGAGGGGTAAATTTTATTGGCATGTAGGTGCAAATGGGCTTGCTGCACCAAAGGATTTCCTTGCCCCTGTAGCCTGGTTCGAAAAGAGTTTTCGTTCTGGTTATACAATTGTTCAAAAGTTTGGCGGGGAACTGTTTACTGCAATACAGGACTTTTCTCCCTTTAATGTAGTTGCTTGGCATGGTAGTTATGTTCCCTATAAGGTGGGTCACTTATGCTGTAGCAGCTAGGCTTCACTCTTGTTTCTTCTTGTGAAATTCTAGAAATAGGCTTCTGCCTATACTTTCTTACAGGCTTTTAGCTGTTTATGTAATATTATAGATTAATGTTAAGTGCTGATCCCACCTCAATAGACCTGAAAGTTGATCCTTCTCCTTGCAGTACGATCTTAGTAAGTTCTGTCCTTACAATACTGTGTTGTTTGATCACGGTGATCCATCGATAAATACAGGTACGTATATATTAATTCCTTCATTCATTTTGCTTAAACGTTCTCCATTGAATTTTACTGCTTTAAAATCCTTGATCTTTTCTTGAATTTCAACTGTTTATTCAACTGTTTGAATTATCATACTGCTGCCCTGTTGCAGTGTTAACAGCACCAACTGATAAACCTGGAGTAGCATTACTCGACTTTGTCATTTTCCCTCCAAGATGGCTTGTTGCTGAACACACATTCCGTCCTCCATATTACCATCGCAATTGCATGAGTGAGTTCATGGGTCTGATTTATGGTGGCTACGAGGTAAATTTCGCAATTTCGTTTTAGATAATGAGGTTGGACCTAAATGTTTAGAGAAGAAAGAGAAGTTGCTCGCTTTATTATTACCCTACTTTGATTTTCATTCTATTCTAATTTCGGATTAGTATCTTAGATGCTCTTAAAAGTCTCTCTTTGCCTGTTACCTTAATTAAAATGTTGTTTTTAGCAGAATATTGGATCTGACTCCCCTTTATATGATACTACTTGTGATGGTTCTGGTTCTAGATATCAAGGGTTATTTTTCTTTTTATAGCTGAGTTTTTTTTCCCCTATCAAGATCTTCAGAATTTCTGATCCATTTTTCAGGCAAAAGCTGATGGGTTCCTTCCTGGCGGTGCCAGCCTTCATAATTGCATGACTCCCCATGGTCCTGATACAAAAACATATGAGGTATGCATTGCTTCTTATAAGTTCCATAGTTCATTCAATTTTCACAACATTCAAACTTTCTACCATACAATTTTCCTTGGTAGGAATTTTTTATTACAAATTCAACCTCCCATAGAAAAATATCGCATGTTTTTGAAGTTGAAGGTATGAACCGTAGAAGTATTGTTTTTCTTCTATAATTGGTATGATTCGTGAAATTAGATGTTTGTGTTTGGTTCATCTTTTTCAATTCTATAAATAGCGATGAACTTTCTTTACTTGGCAAGCTACAAAATTGGTCACCTCATTCTTAAAACTGTTCATGGTTTTAGTTTTAGTCAAATCCAACATTTATGTTTTGGTGAAAAGTCAATGATCTGGTAAATGTTCTCTTGATTCAGGCTACAATTGCTCGAGGGAATGAGGCTGGACCATATAGAATCACCGACACGATGGCATTTATGTTTGAATCGAGTCTGATCCCTCGCGTATGCGCTTGGGCTCTCGAGTCTCCATTCATGGATCGCGACTATTACCGATGCTGGATAGGACTGAAATCTCATTTTACGAACAAAGAAACTAGAGACACTGATCCAAAGAAGGTAAAAACTGAGGCTGGAAACGGAAGACAAATTGGGTAAATATATATTGGATGGAGATCGTGGATTTCATGAAAAAGGGCTCCATCCAAAGTGTATATAGGAATAAAATGATGAATAAGTGACCACACAAGAAAGAATGTGCTTTTTGCTGCTATGGCTGTGGCCTGTTTATAGCCCGAGGAGATCCCCAACGTCCTGATTTCGTGCCTCTCCTCCCTCAACCCCAACAACAATTTATTAGAAATGAAAATAGTCCCGCTTTTATTGACCCCTTCCTGATAAGAAAATACATCTTCAATATCGTCAAATGATTCCATTTAAAAGAAAGTACAACCCTCTTTGATTAGTCTTGAATCGGACGTTATTACTTATATCCCATGATATGTTTGGTCATTTACCTGTATTTCCTCATTTGTTTCTGTTTATAGATTACTTTCGTGACATGTATTTTATATCTTTGAAGATAAAAGAAAAAGTTTAGAAGTTTAGGAGTTGGCTCATACACTACAGATATCAATATATTTTATTATTATTATTGTAAAG

mRNA sequence

ATCAAAAAAAAAAAGAAATTAAAACTTTAATTGGGGGATTAGAGGATAATTAAACTGTTTCCCAATTTCGTCAAAATGTCACCATCAAGTTCTGAGTTCATCTCTATTTTTCTTTACGGCGATGGTGAGCTCCCCAAACGCTATATGACGTACTGGTTTTGATCGTGAATTCAGTTACGTGGGGAAACTTTCCGACGACCGGGACCGCCGCCAGTTCTCCGGCGCCGCCAAATCTCTGCAATCGATCTTCGTCGTCATCTCCAATGGCTGCTCAATCGGTCAACGAATCGGACGGCAGAGATTTCCCTTCCGACCTCGCCTACCAGTCCGGCTTCAACAATCATTTCTCGTCGGAGGCGATTCCCGGCGCTCTTCCTGAATCCCAAAATAGTCCTCTCATCTGCCCCTACGGCCTCTATGCTGAGCAGATCTCCGGCACTTCATTCACCTCGCCTCGAAAAGCAAACTTGCGCAGTTGGCTGTATCGGATAAAGCCCTCGGTCACGCATGAACCGTTTAAGCCACGCTTGCCTAAGAACCAGAAGTTGATCAGTGAATTTAATGATTCGAGTTGTTCCTCGACTCCGACTCAGCTCAGGTGGAAACCGGCGGATTTTCCTGATTCGCCGGTAGATTTCGTTGATGGACTGTACACTGTCTGTGGAGCCGGCAGTTCGTTTCTTCGGCATGGGTTTGCTGTTCACATGTACACAGCCAATAAGTCGATGGAGAACTGTGCGTTCTGTAATGCTGACGGCGACTTCTTGATAGTCCCTCAGAGTGGAAGGCTGTGGATTATTACAGAGTGTGGAAGGCTGGAAGTTTCTCCAGGTGAAATAGTGGTTTTACCTCAAGGTTTTCGCTTTGTTGTTCATCTACCCGATGGGCCATCACGTGGCTACATAGCTGAGATTTTTGGTAGTCATTTTCAGCTTCCTGATCTTGGACCAATAGGTGCAAATGGGCTTGCTGCACCAAAGGATTTCCTTGCCCCTGTAGCCTGGTTCGAAAAGAGTTTTCGTTCTGGTTATACAATTGTTCAAAAGTTTGGCGGGGAACTGTTTACTGCAATACAGGACTTTTCTCCCTTTAATGTAGTTGCTTGGCATGGTAGTTATGTTCCCTATAAGTACGATCTTAGTAAGTTCTGTCCTTACAATACTGTGTTGTTTGATCACGGTGATCCATCGATAAATACAGTGTTAACAGCACCAACTGATAAACCTGGAGTAGCATTACTCGACTTTGTCATTTTCCCTCCAAGATGGCTTGTTGCTGAACACACATTCCGTCCTCCATATTACCATCGCAATTGCATGAGTGAGTTCATGGGTCTGATTTATGGTGGCTACGAGGCAAAAGCTGATGGGTTCCTTCCTGGCGGTGCCAGCCTTCATAATTGCATGACTCCCCATGGTCCTGATACAAAAACATATGAGGCTACAATTGCTCGAGGGAATGAGGCTGGACCATATAGAATCACCGACACGATGGCATTTATGTTTGAATCGAGTCTGATCCCTCGCGTATGCGCTTGGGCTCTCGAGTCTCCATTCATGGATCGCGACTATTACCGATGCTGGATAGGACTGAAATCTCATTTTACGAACAAAGAAACTAGAGACACTGATCCAAAGAAGGTAAAAACTGAGGCTGGAAACGGAAGACAAATTGGGTAAATATATATTGGATGGAGATCGTGGATTTCATGAAAAAGGGCTCCATCCAAAGTGTATATAGGAATAAAATGATGAATAAGTGACCACACAAGAAAGAATGTGCTTTTTGCTGCTATGGCTGTGGCCTGTTTATAGCCCGAGGAGATCCCCAACGTCCTGATTTCGTGCCTCTCCTCCCTCAACCCCAACAACAATTTATTAGAAATGAAAATAGTCCCGCTTTTATTGACCCCTTCCTGATAAGAAAATACATCTTCAATATCGTCAAATGATTCCATTTAAAAGAAAGTACAACCCTCTTTGATTAGTCTTGAATCGGACGTTATTACTTATATCCCATGATATGTTTGGTCATTTACCTGTATTTCCTCATTTGTTTCTGTTTATAGATTACTTTCGTGACATGTATTTTATATCTTTGAAGATAAAAGAAAAAGTTTAGAAGTTTAGGAGTTGGCTCATACACTACAGATATCAATATATTTTATTATTATTATTGTAAAG

Coding sequence (CDS)

ATGGCTGCTCAATCGGTCAACGAATCGGACGGCAGAGATTTCCCTTCCGACCTCGCCTACCAGTCCGGCTTCAACAATCATTTCTCGTCGGAGGCGATTCCCGGCGCTCTTCCTGAATCCCAAAATAGTCCTCTCATCTGCCCCTACGGCCTCTATGCTGAGCAGATCTCCGGCACTTCATTCACCTCGCCTCGAAAAGCAAACTTGCGCAGTTGGCTGTATCGGATAAAGCCCTCGGTCACGCATGAACCGTTTAAGCCACGCTTGCCTAAGAACCAGAAGTTGATCAGTGAATTTAATGATTCGAGTTGTTCCTCGACTCCGACTCAGCTCAGGTGGAAACCGGCGGATTTTCCTGATTCGCCGGTAGATTTCGTTGATGGACTGTACACTGTCTGTGGAGCCGGCAGTTCGTTTCTTCGGCATGGGTTTGCTGTTCACATGTACACAGCCAATAAGTCGATGGAGAACTGTGCGTTCTGTAATGCTGACGGCGACTTCTTGATAGTCCCTCAGAGTGGAAGGCTGTGGATTATTACAGAGTGTGGAAGGCTGGAAGTTTCTCCAGGTGAAATAGTGGTTTTACCTCAAGGTTTTCGCTTTGTTGTTCATCTACCCGATGGGCCATCACGTGGCTACATAGCTGAGATTTTTGGTAGTCATTTTCAGCTTCCTGATCTTGGACCAATAGGTGCAAATGGGCTTGCTGCACCAAAGGATTTCCTTGCCCCTGTAGCCTGGTTCGAAAAGAGTTTTCGTTCTGGTTATACAATTGTTCAAAAGTTTGGCGGGGAACTGTTTACTGCAATACAGGACTTTTCTCCCTTTAATGTAGTTGCTTGGCATGGTAGTTATGTTCCCTATAAGTACGATCTTAGTAAGTTCTGTCCTTACAATACTGTGTTGTTTGATCACGGTGATCCATCGATAAATACAGTGTTAACAGCACCAACTGATAAACCTGGAGTAGCATTACTCGACTTTGTCATTTTCCCTCCAAGATGGCTTGTTGCTGAACACACATTCCGTCCTCCATATTACCATCGCAATTGCATGAGTGAGTTCATGGGTCTGATTTATGGTGGCTACGAGGCAAAAGCTGATGGGTTCCTTCCTGGCGGTGCCAGCCTTCATAATTGCATGACTCCCCATGGTCCTGATACAAAAACATATGAGGCTACAATTGCTCGAGGGAATGAGGCTGGACCATATAGAATCACCGACACGATGGCATTTATGTTTGAATCGAGTCTGATCCCTCGCGTATGCGCTTGGGCTCTCGAGTCTCCATTCATGGATCGCGACTATTACCGATGCTGGATAGGACTGAAATCTCATTTTACGAACAAAGAAACTAGAGACACTGATCCAAAGAAGGTAAAAACTGAGGCTGGAAACGGAAGACAAATTGGGTAA

Protein sequence

MAAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTSFTSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPDSPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIITECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKDFLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNTVLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIPRVCAWALESPFMDRDYYRCWIGLKSHFTNKETRDTDPKKVKTEAGNGRQIG
Homology
BLAST of MC10g0912 vs. ExPASy Swiss-Prot
Match: Q9ZRA2 (Homogentisate 1,2-dioxygenase OS=Arabidopsis thaliana OX=3702 GN=HGO PE=2 SV=2)

HSP 1 Score: 785.8 bits (2028), Expect = 2.7e-226
Identity = 356/449 (79.29%), Postives = 393/449 (87.53%), Query Frame = 0

Query: 17  DLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTSFTSPRKANLRSWLYRI 76
           +L YQSGF NHFSSEAI GALP  QNSPL+CPYGLYAEQISGTSFTSPRK N RSWLYR+
Sbjct: 10  ELKYQSGFGNHFSSEAIAGALPLDQNSPLLCPYGLYAEQISGTSFTSPRKLNQRSWLYRV 69

Query: 77  KPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPDSPVDFVDGLYTVCGAG 136
           KPSVTHEPFKPR+P ++KL+SEF+ S+  + PTQLRW+P D PDS +DFVDGL+T+CGAG
Sbjct: 70  KPSVTHEPFKPRVPAHKKLVSEFDASNSRTNPTQLRWRPEDIPDSEIDFVDGLFTICGAG 129

Query: 137 SSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIITECGRLEVSPGEIVVLP 196
           SSFLRHGFA+HMY AN  M++ AFCNADGDFL+VPQ+GRLWI TECGRL V+PGEI V+P
Sbjct: 130 SSFLRHGFAIHMYVANTGMKDSAFCNADGDFLLVPQTGRLWIETECGRLLVTPGEIAVIP 189

Query: 197 QGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKDFLAPVAWFEKSFRSGY 256
           QGFRF + LPDG SRGY+AEI+G+HFQLPDLGPIGANGLAA +DFLAP AWFE   R  Y
Sbjct: 190 QGFRFSIDLPDGKSRGYVAEIYGAHFQLPDLGPIGANGLAASRDFLAPTAWFEDGLRPEY 249

Query: 257 TIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNTVLFDHGDPSINTVLTA 316
           TIVQKFGGELFTA QDFSPFNVVAWHG+YVPYKYDL KFCPYNTVL DHGDPSINTVLTA
Sbjct: 250 TIVQKFGGELFTAKQDFSPFNVVAWHGNYVPYKYDLKKFCPYNTVLLDHGDPSINTVLTA 309

Query: 317 PTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFLPGGAS 376
           PTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYG YEAKADGFLPGGAS
Sbjct: 310 PTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGAYEAKADGFLPGGAS 369

Query: 377 LHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIPRVCAWALESPFMDRDY 436
           LH+CMTPHGPDT TYEATIAR N   P ++T TMAFMFES+LIPRVC WALESPF+D DY
Sbjct: 370 LHSCMTPHGPDTTTYEATIARVNAMAPSKLTGTMAFMFESALIPRVCHWALESPFLDHDY 429

Query: 437 YRCWIGLKSHFTNKETRDTDPKKVKTEAG 466
           Y+CWIGLKSHF+      T+ +  + E G
Sbjct: 430 YQCWIGLKSHFSRISLDKTNVESTEKEPG 458

BLAST of MC10g0912 vs. ExPASy Swiss-Prot
Match: Q5VRH4 (Homogentisate 1,2-dioxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=HGO PE=2 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 3.8e-212
Identity = 339/452 (75.00%), Postives = 386/452 (85.40%), Query Frame = 0

Query: 2   AAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTSF 61
           AAQ+  +  G     +  Y SG  N  SSEA+ G LP  QNSPL+CP GLYAEQ+SGT F
Sbjct: 9   AAQNEQQEKG---GLEYVYLSGLGNSLSSEAVAGTLPRGQNSPLVCPLGLYAEQLSGTPF 68

Query: 62  TSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCS--STPTQLRWKPADFP 121
           T+PR  NLR+WLYRIKPSVTHEPF PR P + +LI +F+ ++    +TPTQLRW+PAD P
Sbjct: 69  TAPRARNLRTWLYRIKPSVTHEPFHPRRPAHPRLIGDFDRTTTDTVATPTQLRWRPADVP 128

Query: 122 --DSPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLW 181
               P+DF+DGLYTVCGAGSSFLRHG+A+HMY ANKSM+ CAFCNADGDFLIVPQ G+L 
Sbjct: 129 PHHPPLDFIDGLYTVCGAGSSFLRHGYAIHMYAANKSMDGCAFCNADGDFLIVPQQGKLL 188

Query: 182 IITECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAA 241
           I TECG+L V PGEIVV+PQGFRF V LPDGPSRGY++EIFG+HFQLPDLGPIGANGLA+
Sbjct: 189 ITTECGKLLVPPGEIVVIPQGFRFAVDLPDGPSRGYVSEIFGTHFQLPDLGPIGANGLAS 248

Query: 242 PKDFLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCP 301
            +DFL+P AWFE+  R GYTIVQK+GGELFTA QDFSPFNVVAWHG+YVPYKYDLSKFCP
Sbjct: 249 ARDFLSPTAWFEQVHRPGYTIVQKYGGELFTATQDFSPFNVVAWHGNYVPYKYDLSKFCP 308

Query: 302 YNTVLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMG 361
           +NTVLFDH DPS+NTVLTAPTDKPGVALLDFVIFPPRWLVAE+TFRPPYYHRNCMSEFMG
Sbjct: 309 FNTVLFDHADPSVNTVLTAPTDKPGVALLDFVIFPPRWLVAENTFRPPYYHRNCMSEFMG 368

Query: 362 LIYGGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESS 421
           LIYG YEAKADGFLPGGASLH+CMTPHGPDTKTYEATI+R +   P R++ T+AFMFES+
Sbjct: 369 LIYGIYEAKADGFLPGGASLHSCMTPHGPDTKTYEATISRPDANEPSRLSGTLAFMFESA 428

Query: 422 LIPRVCAWALESPFMDRDYYRCWIGLKSHFTN 450
           LIPRVC WAL+SP  D DYY+CWIGLKSHF++
Sbjct: 429 LIPRVCQWALDSPSRDLDYYQCWIGLKSHFSH 457

BLAST of MC10g0912 vs. ExPASy Swiss-Prot
Match: Q54QI4 (Homogentisate 1,2-dioxygenase OS=Dictyostelium discoideum OX=44689 GN=hgd PE=2 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 6.0e-149
Identity = 263/434 (60.60%), Postives = 320/434 (73.73%), Query Frame = 0

Query: 17  DLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTSFTSPRKANLRSWLYRI 76
           D  YQSGF N F SEAI G LP+ +N+P  CP  LYAEQ+SG +FT+PR    RSWLYRI
Sbjct: 7   DYEYQSGFGNSFESEAIKGTLPKGRNAPQNCPLDLYAEQLSGNAFTAPRHTQQRSWLYRI 66

Query: 77  KPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFP---DSPVDFVDGLYTVC 136
           +PSV H P K   P +  L+ + N+      P QLRWKP  FP   D P DFV+GL T+ 
Sbjct: 67  RPSVCHTPLK---PIDSGLVCDLNNLHVD--PNQLRWKP--FPITEDKPHDFVEGLITIA 126

Query: 137 GAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIITECGRLEVSPGEIV 196
           GAG + +RHG A+H+YTA KSMEN +F N+DGDFLIVPQ G L I TE G ++V  GEI 
Sbjct: 127 GAGHASVRHGLAIHIYTATKSMENKSFYNSDGDFLIVPQQGTLDIQTEFGFMKVKSGEIC 186

Query: 197 VLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKDFLAPVAWFEKSFR 256
           V+ +G  F V++ +GP+RGYI E+FGSHF+LPDLGPIGANGLA P+DFL+PVA +EK   
Sbjct: 187 VIQRGITFSVNV-EGPTRGYICEVFGSHFKLPDLGPIGANGLANPRDFLSPVAAYEKKEG 246

Query: 257 SGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNTVLFDHGDPSINTV 316
             +T + KF G+LF+A Q +SPFNVVAWHG+Y PYKYDLS FC  N+V FDH DPSI TV
Sbjct: 247 IEHTKINKFLGKLFSATQTYSPFNVVAWHGNYCPYKYDLSLFCVVNSVSFDHLDPSIFTV 306

Query: 317 LTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFLPG 376
           LTAPT++ GVA  DFVIFPPRWLV E+TFRPPY+HRNCMSEFMGLI G YEAK +GFLPG
Sbjct: 307 LTAPTNEVGVAAADFVIFPPRWLVQENTFRPPYFHRNCMSEFMGLIRGVYEAKKEGFLPG 366

Query: 377 GASLHNCMTPHGPDTKTYEATIARGNEAGPYRITD-TMAFMFESSLIPRVCAWALESPFM 436
           G SLH+CMTPHGPD+ T+ A I    E  P +I D  +AFMFESSLI  +  +A ++ F+
Sbjct: 367 GGSLHSCMTPHGPDSDTFYAAIKA--ELKPTKIPDVALAFMFESSLILGISDYAKKN-FI 426

Query: 437 DRDYYRCWIGLKSH 447
           D DY++CW GLK +
Sbjct: 427 DDDYWKCWQGLKDN 429

BLAST of MC10g0912 vs. ExPASy Swiss-Prot
Match: O09173 (Homogentisate 1,2-dioxygenase OS=Mus musculus OX=10090 GN=Hgd PE=1 SV=2)

HSP 1 Score: 517.3 bits (1331), Expect = 1.8e-145
Identity = 257/451 (56.98%), Postives = 314/451 (69.62%), Query Frame = 0

Query: 16  SDLAYQSGFNNHFSSE--AIPGALPESQNSPLICPYGLYAEQISGTSFTSPRKANLRSWL 75
           ++L Y SGF N  +SE    PG+LP+ QN+P +CPY LYAEQ+SG++FT PR  N RSWL
Sbjct: 2   AELKYISGFGNECASEDPRCPGSLPKGQNNPQVCPYNLYAEQLSGSAFTCPRNTNKRSWL 61

Query: 76  YRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFP---DSPVDFVDGLY 135
           YRI PSV+H+PF+     +Q  ++  N       P QLRWKP + P   +  VDFV GLY
Sbjct: 62  YRILPSVSHKPFE---SIDQGHVTH-NWDEVGPDPNQLRWKPFEIPKASEKKVDFVSGLY 121

Query: 136 TVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIITECGRLEVSPG 195
           T+CGAG     +G AVH++  N SMEN  F N+DGDFLIVPQ G+L I TE G++ + P 
Sbjct: 122 TLCGAGDIKSNNGLAVHIFLCNSSMENRCFYNSDGDFLIVPQKGKLLIYTEFGKMSLQPN 181

Query: 196 EIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKDFLAPVAWFE- 255
           EI V+ +G RF V + +  +RGYI E++G HF+LPDLGPIGANGLA P+DFL PVAW+E 
Sbjct: 182 EICVIQRGMRFSVDVFE-ETRGYILEVYGVHFELPDLGPIGANGLANPRDFLIPVAWYED 241

Query: 256 KSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNTVLFDHGDPS 315
           +    GYT++ KF G+LF   QD SPFNVVAWHG+Y PYKY+L  F   N V FDH DPS
Sbjct: 242 RRVPGGYTVINKFQGKLFACKQDVSPFNVVAWHGNYTPYKYNLENFMVINAVAFDHADPS 301

Query: 316 INTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADG 375
           I TVLTA + +PGVA+ DFVIFPPRW VA+ TFRPPYYHRNCMSEFMGLI G YEAK  G
Sbjct: 302 IFTVLTAKSLRPGVAIADFVIFPPRWGVADKTFRPPYYHRNCMSEFMGLIKGHYEAKQGG 361

Query: 376 FLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITD-TMAFMFESSLIPRVCAWALE 435
           FLPGG SLH+ MTPHGPD   +E   A   +  P RI D TMAFMFESSL   V  W L+
Sbjct: 362 FLPGGGSLHSAMTPHGPDADCFEK--ASKAKLEPERIADGTMAFMFESSLSLAVTKWGLK 421

Query: 436 S-PFMDRDYYRCWIGLKSHFTNKETRDTDPK 459
           +   +D +YY+CW  L+SHFT      T+PK
Sbjct: 422 TCSCLDENYYKCWEPLRSHFTPNSRSPTEPK 445

BLAST of MC10g0912 vs. ExPASy Swiss-Prot
Match: Q93099 (Homogentisate 1,2-dioxygenase OS=Homo sapiens OX=9606 GN=HGD PE=1 SV=2)

HSP 1 Score: 513.5 bits (1321), Expect = 2.6e-144
Identity = 255/450 (56.67%), Postives = 311/450 (69.11%), Query Frame = 0

Query: 16  SDLAYQSGFNNHFSSE--AIPGALPESQNSPLICPYGLYAEQISGTSFTSPRKANLRSWL 75
           ++L Y SGF N  SSE    PG+LPE QN+P +CPY LYAEQ+SG++FT PR  N RSWL
Sbjct: 2   AELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSWL 61

Query: 76  YRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFP---DSPVDFVDGLY 135
           YRI PSV+H+PF+  + + Q      N       P QLRWKP + P      VDFV GL+
Sbjct: 62  YRILPSVSHKPFE-SIDEGQ---VTHNWDEVDPDPNQLRWKPFEIPKASQKKVDFVSGLH 121

Query: 136 TVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIITECGRLEVSPG 195
           T+CGAG     +G A+H++  N SMEN  F N+DGDFLIVPQ G L I TE G++ V P 
Sbjct: 122 TLCGAGDIKSNNGLAIHIFLCNTSMENRCFYNSDGDFLIVPQKGNLLIYTEFGKMLVQPN 181

Query: 196 EIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKDFLAPVAWFE- 255
           EI V+ +G RF + + +  +RGYI E++G HF+LPDLGPIGANGLA P+DFL P+AW+E 
Sbjct: 182 EICVIQRGMRFSIDVFE-ETRGYILEVYGVHFELPDLGPIGANGLANPRDFLIPIAWYED 241

Query: 256 KSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNTVLFDHGDPS 315
           +    GYT++ K+ G+LF A QD SPFNVVAWHG+Y PYKY+L  F   N+V FDH DPS
Sbjct: 242 RQVPGGYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLKNFMVINSVAFDHADPS 301

Query: 316 INTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADG 375
           I TVLTA + +PGVA+ DFVIFPPRW VA+ TFRPPYYHRNCMSEFMGLI G YEAK  G
Sbjct: 302 IFTVLTAKSVRPGVAIADFVIFPPRWGVADKTFRPPYYHRNCMSEFMGLIRGHYEAKQGG 361

Query: 376 FLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITD-TMAFMFESSLIPRVCAWALE 435
           FLPGG SLH+ MTPHGPD   +E   A   +  P RI D TMAFMFESSL   V  W L+
Sbjct: 362 FLPGGGSLHSTMTPHGPDADCFEK--ASKVKLAPERIADGTMAFMFESSLSLAVTKWGLK 421

Query: 436 -SPFMDRDYYRCWIGLKSHFTNKETRDTDP 458
            S  +D +Y++CW  LKSHFT       +P
Sbjct: 422 ASRCLDENYHKCWEPLKSHFTPNSRNPAEP 444

BLAST of MC10g0912 vs. NCBI nr
Match: XP_022142871.1 (homogentisate 1,2-dioxygenase [Momordica charantia])

HSP 1 Score: 1005 bits (2598), Expect = 0.0
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 1   MAAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTS 60
           MAAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTS
Sbjct: 1   MAAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTS 60

Query: 61  FTSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPD 120
           FTSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPD
Sbjct: 61  FTSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPD 120

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT
Sbjct: 121 SPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKD 240
           ECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKD
Sbjct: 181 ECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKD 240

Query: 241 FLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNT 300
           FLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNT
Sbjct: 241 FLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNT 300

Query: 301 VLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY
Sbjct: 301 VLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360

Query: 361 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 420
           GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP
Sbjct: 361 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 420

Query: 421 RVCAWALESPFMDRDYYRCWIGLKSHFTNKETRDTDPKKVKTEAGNGRQIG 471
           RVCAWALESPFMDRDYYRCWIGLKSHFTNKETRDTDPKKVKTEAGNGRQIG
Sbjct: 421 RVCAWALESPFMDRDYYRCWIGLKSHFTNKETRDTDPKKVKTEAGNGRQIG 471

BLAST of MC10g0912 vs. NCBI nr
Match: XP_022980282.1 (homogentisate 1,2-dioxygenase [Cucurbita maxima])

HSP 1 Score: 926 bits (2392), Expect = 0.0
Identity = 429/470 (91.28%), Postives = 450/470 (95.74%), Query Frame = 0

Query: 1   MAAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTS 60
           MAAQSV E DG +FPSDLAYQSGFNNHFSSEAIPGALP+ QNSPLICPYGLYAEQISGTS
Sbjct: 58  MAAQSVGEMDGENFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTS 117

Query: 61  FTSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPD 120
           FTSPRK N+ SWLYRIKPSVTHEPF+PRLPKN+KLISEFN S+CSSTPTQLRW+PAD PD
Sbjct: 118 FTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPADVPD 177

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SP+DFVDGLYTVCGAGSSFLRHGFA+HMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT
Sbjct: 178 SPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 237

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKD 240
           ECGRLEVSPGEIVVLPQGFRFVV+LPDGPSRGY+AEIFGSHFQLPDLGPIGANGLAAP+D
Sbjct: 238 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 297

Query: 241 FLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNT 300
           FLAPVAWFE   R GYTIVQK+GGELFTAIQDFSPFNVVAWHG+YVPYKYDLSKFCPYNT
Sbjct: 298 FLAPVAWFENISRPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 357

Query: 301 VLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDH DPSINTVLTAPTDKPGVALLDFVIFPPRWLV+EHTFRPPYYHRNCMSEFMGLIY
Sbjct: 358 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIY 417

Query: 361 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 420
           GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP
Sbjct: 418 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 477

Query: 421 RVCAWALESPFMDRDYYRCWIGLKSHFTNK-ETRDTDPKKVKTEAGNGRQ 469
           RVC+WALESP MD DYY+CWIGLKSHFT++  TRD DP+KVKT+  NGRQ
Sbjct: 478 RVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPRKVKTDVENGRQ 527

BLAST of MC10g0912 vs. NCBI nr
Match: KAG6600393.1 (Homogentisate 1,2-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 923 bits (2385), Expect = 0.0
Identity = 428/470 (91.06%), Postives = 450/470 (95.74%), Query Frame = 0

Query: 1   MAAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTS 60
           MAAQSV E DG +FPSDLAYQSGFNNHFSSEAIPGALP+ QNSPLICPYGLYAEQISGTS
Sbjct: 1   MAAQSVGEMDGGNFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTS 60

Query: 61  FTSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPD 120
           FTSPRK N+ SWLYRIKPSVTHEPF+PRLPKN+KLISEFN S+CSSTPTQLRW+PA+ PD
Sbjct: 61  FTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPD 120

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SP+DFVDGLYTVCGAGSSFLRHGFA+HMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT
Sbjct: 121 SPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKD 240
           ECGRLEVSPGEIVVLPQGFRFVV+LPDGPSRGY+AEIFGSHFQLPDLGPIGANGLAAP+D
Sbjct: 181 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240

Query: 241 FLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNT 300
           FLAPVAWFE   R GYTIVQK+GGELFTAIQDFSPFNVVAWHG+YVPYKYDLSKFCPYNT
Sbjct: 241 FLAPVAWFENISRPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 300

Query: 301 VLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDH DPSINTVLTAPTDKPGVALLDFVIFPPRWLV+EHTFRPPYYHRNCMSEFMGLIY
Sbjct: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIY 360

Query: 361 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 420
           GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP
Sbjct: 361 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 420

Query: 421 RVCAWALESPFMDRDYYRCWIGLKSHFTNK-ETRDTDPKKVKTEAGNGRQ 469
           RVC+WALESP MD DYY+CWIGLKSHFT++  TRD DP+KVKT+  NGRQ
Sbjct: 421 RVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPQKVKTDVENGRQ 470

BLAST of MC10g0912 vs. NCBI nr
Match: XP_004137214.1 (homogentisate 1,2-dioxygenase [Cucumis sativus])

HSP 1 Score: 922 bits (2382), Expect = 0.0
Identity = 424/471 (90.02%), Postives = 451/471 (95.75%), Query Frame = 0

Query: 1   MAAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTS 60
           MAAQSV E+DG DFPSDL Y SGFNNHFSSEAIPGALP+SQNSPLICP+GLYAEQISGTS
Sbjct: 1   MAAQSVGETDGTDFPSDLPYLSGFNNHFSSEAIPGALPQSQNSPLICPFGLYAEQISGTS 60

Query: 61  FTSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPD 120
           FTSPRKANL SWLYRIKPSVTHEPF+ RLPKN+KLISEFN S+CSSTPTQLRWKPADFPD
Sbjct: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCSSTPTQLRWKPADFPD 120

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SPVDFVDGLYTVCGAGSSFLRHGFA+HMYTANKSMENCAFCNADGDFLIVPQSG+LWIIT
Sbjct: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGKLWIIT 180

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKD 240
           ECGRLEVSPGE+VVLPQGFRFVV+LPDGPSRGY+AEIFGSHFQLPDLGPIGANGLAAP+D
Sbjct: 181 ECGRLEVSPGEVVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240

Query: 241 FLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNT 300
           FLAPVAWFE S R GYTI+QKFGGELFTAIQDFSPFNVVAWHG+YVPYKYDL KFCPYNT
Sbjct: 241 FLAPVAWFENSPRPGYTIIQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLCKFCPYNT 300

Query: 301 VLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDH DPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY
Sbjct: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360

Query: 361 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 420
           GGYEAKADGF+PGGASLH+CMTPHGPDTKTYEATIARGN+AGP++I+ TMAFMFESSLIP
Sbjct: 361 GGYEAKADGFVPGGASLHSCMTPHGPDTKTYEATIARGNDAGPHKISGTMAFMFESSLIP 420

Query: 421 RVCAWALESPFMDRDYYRCWIGLKSHFTNKETRDTDPKKVKTEAGNGRQIG 471
           RVC+WALESPF+D DYY+CWIGLKSHF N+   DTDP+KV+ E+ NGRQIG
Sbjct: 421 RVCSWALESPFIDHDYYQCWIGLKSHFKNEAIGDTDPQKVRIESENGRQIG 471

BLAST of MC10g0912 vs. NCBI nr
Match: XP_022942212.1 (homogentisate 1,2-dioxygenase [Cucurbita moschata])

HSP 1 Score: 921 bits (2381), Expect = 0.0
Identity = 427/470 (90.85%), Postives = 449/470 (95.53%), Query Frame = 0

Query: 1   MAAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTS 60
           MAAQSV E DG +FPSDLAYQSGFNNHFSSEAIPGALP+ QNSPLICPYGLYAEQISGTS
Sbjct: 1   MAAQSVGEMDGGNFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTS 60

Query: 61  FTSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPD 120
           FTSPRK N+ SWLYRIKPSVTHEPF+PRLPKN+KLISEFN S+CSSTPTQLRW+PA+ PD
Sbjct: 61  FTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPD 120

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SP+DFVDGLYTVCGAGSSFLRHGFA+HMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT
Sbjct: 121 SPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKD 240
           ECGRLEVSPGEIVVLPQGFRFVV+LPDGPSRGY+AEIFGSHFQLPDLGPIGANGLAAP+D
Sbjct: 181 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240

Query: 241 FLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNT 300
           FLAPVAWFE   R GYTIVQK+GGE FTAIQDFSPFNVVAWHG+YVPYKYDLSKFCPYNT
Sbjct: 241 FLAPVAWFENLSRPGYTIVQKYGGEFFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 300

Query: 301 VLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDH DPSINTVLTAPTDKPGVALLDFVIFPPRWLV+EHTFRPPYYHRNCMSEFMGLIY
Sbjct: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIY 360

Query: 361 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 420
           GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP
Sbjct: 361 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 420

Query: 421 RVCAWALESPFMDRDYYRCWIGLKSHFTNK-ETRDTDPKKVKTEAGNGRQ 469
           RVC+WALESP MD DYY+CWIGLKSHFT++  TRD DP+KVKT+  NGRQ
Sbjct: 421 RVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPQKVKTDVENGRQ 470

BLAST of MC10g0912 vs. ExPASy TrEMBL
Match: A0A6J1CNH2 (Homogentisate 1,2-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111012880 PE=3 SV=1)

HSP 1 Score: 1005 bits (2598), Expect = 0.0
Identity = 471/471 (100.00%), Postives = 471/471 (100.00%), Query Frame = 0

Query: 1   MAAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTS 60
           MAAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTS
Sbjct: 1   MAAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTS 60

Query: 61  FTSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPD 120
           FTSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPD
Sbjct: 61  FTSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPD 120

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT
Sbjct: 121 SPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKD 240
           ECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKD
Sbjct: 181 ECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKD 240

Query: 241 FLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNT 300
           FLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNT
Sbjct: 241 FLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNT 300

Query: 301 VLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY
Sbjct: 301 VLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360

Query: 361 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 420
           GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP
Sbjct: 361 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 420

Query: 421 RVCAWALESPFMDRDYYRCWIGLKSHFTNKETRDTDPKKVKTEAGNGRQIG 471
           RVCAWALESPFMDRDYYRCWIGLKSHFTNKETRDTDPKKVKTEAGNGRQIG
Sbjct: 421 RVCAWALESPFMDRDYYRCWIGLKSHFTNKETRDTDPKKVKTEAGNGRQIG 471

BLAST of MC10g0912 vs. ExPASy TrEMBL
Match: A0A6J1IQY7 (Homogentisate 1,2-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111479699 PE=3 SV=1)

HSP 1 Score: 926 bits (2392), Expect = 0.0
Identity = 429/470 (91.28%), Postives = 450/470 (95.74%), Query Frame = 0

Query: 1   MAAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTS 60
           MAAQSV E DG +FPSDLAYQSGFNNHFSSEAIPGALP+ QNSPLICPYGLYAEQISGTS
Sbjct: 58  MAAQSVGEMDGENFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTS 117

Query: 61  FTSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPD 120
           FTSPRK N+ SWLYRIKPSVTHEPF+PRLPKN+KLISEFN S+CSSTPTQLRW+PAD PD
Sbjct: 118 FTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPADVPD 177

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SP+DFVDGLYTVCGAGSSFLRHGFA+HMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT
Sbjct: 178 SPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 237

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKD 240
           ECGRLEVSPGEIVVLPQGFRFVV+LPDGPSRGY+AEIFGSHFQLPDLGPIGANGLAAP+D
Sbjct: 238 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 297

Query: 241 FLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNT 300
           FLAPVAWFE   R GYTIVQK+GGELFTAIQDFSPFNVVAWHG+YVPYKYDLSKFCPYNT
Sbjct: 298 FLAPVAWFENISRPGYTIVQKYGGELFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 357

Query: 301 VLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDH DPSINTVLTAPTDKPGVALLDFVIFPPRWLV+EHTFRPPYYHRNCMSEFMGLIY
Sbjct: 358 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIY 417

Query: 361 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 420
           GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP
Sbjct: 418 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 477

Query: 421 RVCAWALESPFMDRDYYRCWIGLKSHFTNK-ETRDTDPKKVKTEAGNGRQ 469
           RVC+WALESP MD DYY+CWIGLKSHFT++  TRD DP+KVKT+  NGRQ
Sbjct: 478 RVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPRKVKTDVENGRQ 527

BLAST of MC10g0912 vs. ExPASy TrEMBL
Match: A0A0A0KYZ6 (Homogentisate 1,2-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_4G088750 PE=3 SV=1)

HSP 1 Score: 922 bits (2382), Expect = 0.0
Identity = 424/471 (90.02%), Postives = 451/471 (95.75%), Query Frame = 0

Query: 1   MAAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTS 60
           MAAQSV E+DG DFPSDL Y SGFNNHFSSEAIPGALP+SQNSPLICP+GLYAEQISGTS
Sbjct: 1   MAAQSVGETDGTDFPSDLPYLSGFNNHFSSEAIPGALPQSQNSPLICPFGLYAEQISGTS 60

Query: 61  FTSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPD 120
           FTSPRKANL SWLYRIKPSVTHEPF+ RLPKN+KLISEFN S+CSSTPTQLRWKPADFPD
Sbjct: 61  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCSSTPTQLRWKPADFPD 120

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SPVDFVDGLYTVCGAGSSFLRHGFA+HMYTANKSMENCAFCNADGDFLIVPQSG+LWIIT
Sbjct: 121 SPVDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGKLWIIT 180

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKD 240
           ECGRLEVSPGE+VVLPQGFRFVV+LPDGPSRGY+AEIFGSHFQLPDLGPIGANGLAAP+D
Sbjct: 181 ECGRLEVSPGEVVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240

Query: 241 FLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNT 300
           FLAPVAWFE S R GYTI+QKFGGELFTAIQDFSPFNVVAWHG+YVPYKYDL KFCPYNT
Sbjct: 241 FLAPVAWFENSPRPGYTIIQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLCKFCPYNT 300

Query: 301 VLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDH DPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY
Sbjct: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360

Query: 361 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 420
           GGYEAKADGF+PGGASLH+CMTPHGPDTKTYEATIARGN+AGP++I+ TMAFMFESSLIP
Sbjct: 361 GGYEAKADGFVPGGASLHSCMTPHGPDTKTYEATIARGNDAGPHKISGTMAFMFESSLIP 420

Query: 421 RVCAWALESPFMDRDYYRCWIGLKSHFTNKETRDTDPKKVKTEAGNGRQIG 471
           RVC+WALESPF+D DYY+CWIGLKSHF N+   DTDP+KV+ E+ NGRQIG
Sbjct: 421 RVCSWALESPFIDHDYYQCWIGLKSHFKNEAIGDTDPQKVRIESENGRQIG 471

BLAST of MC10g0912 vs. ExPASy TrEMBL
Match: A0A6J1FPL5 (Homogentisate 1,2-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111447335 PE=3 SV=1)

HSP 1 Score: 921 bits (2381), Expect = 0.0
Identity = 427/470 (90.85%), Postives = 449/470 (95.53%), Query Frame = 0

Query: 1   MAAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTS 60
           MAAQSV E DG +FPSDLAYQSGFNNHFSSEAIPGALP+ QNSPLICPYGLYAEQISGTS
Sbjct: 1   MAAQSVGEMDGGNFPSDLAYQSGFNNHFSSEAIPGALPQLQNSPLICPYGLYAEQISGTS 60

Query: 61  FTSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPD 120
           FTSPRK N+ SWLYRIKPSVTHEPF+PRLPKN+KLISEFN S+CSSTPTQLRW+PA+ PD
Sbjct: 61  FTSPRKVNMCSWLYRIKPSVTHEPFRPRLPKNEKLISEFNASNCSSTPTQLRWRPAEVPD 120

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SP+DFVDGLYTVCGAGSSFLRHGFA+HMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT
Sbjct: 121 SPLDFVDGLYTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKD 240
           ECGRLEVSPGEIVVLPQGFRFVV+LPDGPSRGY+AEIFGSHFQLPDLGPIGANGLAAP+D
Sbjct: 181 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGSHFQLPDLGPIGANGLAAPRD 240

Query: 241 FLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNT 300
           FLAPVAWFE   R GYTIVQK+GGE FTAIQDFSPFNVVAWHG+YVPYKYDLSKFCPYNT
Sbjct: 241 FLAPVAWFENLSRPGYTIVQKYGGEFFTAIQDFSPFNVVAWHGNYVPYKYDLSKFCPYNT 300

Query: 301 VLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDH DPSINTVLTAPTDKPGVALLDFVIFPPRWLV+EHTFRPPYYHRNCMSEFMGLIY
Sbjct: 301 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVSEHTFRPPYYHRNCMSEFMGLIY 360

Query: 361 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 420
           GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP
Sbjct: 361 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 420

Query: 421 RVCAWALESPFMDRDYYRCWIGLKSHFTNK-ETRDTDPKKVKTEAGNGRQ 469
           RVC+WALESP MD DYY+CWIGLKSHFT++  TRD DP+KVKT+  NGRQ
Sbjct: 421 RVCSWALESPCMDHDYYQCWIGLKSHFTSEGTTRDMDPQKVKTDVENGRQ 470

BLAST of MC10g0912 vs. ExPASy TrEMBL
Match: A0A5A7UWB4 (Homogentisate 1,2-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold233G00260 PE=3 SV=1)

HSP 1 Score: 917 bits (2369), Expect = 0.0
Identity = 421/471 (89.38%), Postives = 450/471 (95.54%), Query Frame = 0

Query: 1   MAAQSVNESDGRDFPSDLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTS 60
           MAAQSV E++GRDFPSDL Y SGFNNHFSSEAIPGALP+SQNSPLICP+GLYAEQISGTS
Sbjct: 22  MAAQSVGETEGRDFPSDLPYLSGFNNHFSSEAIPGALPQSQNSPLICPFGLYAEQISGTS 81

Query: 61  FTSPRKANLRSWLYRIKPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPD 120
           FTSPRKANL SWLYRIKPSVTHEPF+ RLPKN+KLISEFN S+CSSTPTQLRWKPADFPD
Sbjct: 82  FTSPRKANLCSWLYRIKPSVTHEPFRQRLPKNEKLISEFNASNCSSTPTQLRWKPADFPD 141

Query: 121 SPVDFVDGLYTVCGAGSSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIIT 180
           SPVDFVDGL+TVCGAGSSFLRHGFA+HMYTANKSMENCAFCNADGDFLIVPQ+GRLWI T
Sbjct: 142 SPVDFVDGLHTVCGAGSSFLRHGFAIHMYTANKSMENCAFCNADGDFLIVPQTGRLWITT 201

Query: 181 ECGRLEVSPGEIVVLPQGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKD 240
           ECGRLEVSPGEIVVLPQGFRFVV+LPDGPSRGY+AEIFG HFQLPDLGPIGANGLAAP+D
Sbjct: 202 ECGRLEVSPGEIVVLPQGFRFVVYLPDGPSRGYVAEIFGCHFQLPDLGPIGANGLAAPRD 261

Query: 241 FLAPVAWFEKSFRSGYTIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNT 300
           FLAPVAWFE S R GYT++QKFGGELFTAIQDFSPFNVVAWHG+YVPYKYDL KFCPYNT
Sbjct: 262 FLAPVAWFENSPRPGYTVIQKFGGELFTAIQDFSPFNVVAWHGNYVPYKYDLCKFCPYNT 321

Query: 301 VLFDHGDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 360
           VLFDH DPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY
Sbjct: 322 VLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIY 381

Query: 361 GGYEAKADGFLPGGASLHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIP 420
           GGYEAKADGF+PGGASLH+CMTPHGPDTKTYEATIARGN+AGP++I+ TMAFMFESSLIP
Sbjct: 382 GGYEAKADGFVPGGASLHSCMTPHGPDTKTYEATIARGNDAGPHKISGTMAFMFESSLIP 441

Query: 421 RVCAWALESPFMDRDYYRCWIGLKSHFTNKETRDTDPKKVKTEAGNGRQIG 471
           RVC+WALESPFMD DYY+CWIGLKSHF N+   DTDP+KV+ ++ NGRQIG
Sbjct: 442 RVCSWALESPFMDHDYYQCWIGLKSHFKNEAIGDTDPQKVRIKSENGRQIG 492

BLAST of MC10g0912 vs. TAIR 10
Match: AT5G54080.1 (homogentisate 1,2-dioxygenase )

HSP 1 Score: 785.8 bits (2028), Expect = 1.9e-227
Identity = 356/449 (79.29%), Postives = 393/449 (87.53%), Query Frame = 0

Query: 17  DLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTSFTSPRKANLRSWLYRI 76
           +L YQSGF NHFSSEAI GALP  QNSPL+CPYGLYAEQISGTSFTSPRK N RSWLYR+
Sbjct: 10  ELKYQSGFGNHFSSEAIAGALPLDQNSPLLCPYGLYAEQISGTSFTSPRKLNQRSWLYRV 69

Query: 77  KPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPDSPVDFVDGLYTVCGAG 136
           KPSVTHEPFKPR+P ++KL+SEF+ S+  + PTQLRW+P D PDS +DFVDGL+T+CGAG
Sbjct: 70  KPSVTHEPFKPRVPAHKKLVSEFDASNSRTNPTQLRWRPEDIPDSEIDFVDGLFTICGAG 129

Query: 137 SSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIITECGRLEVSPGEIVVLP 196
           SSFLRHGFA+HMY AN  M++ AFCNADGDFL+VPQ+GRLWI TECGRL V+PGEI V+P
Sbjct: 130 SSFLRHGFAIHMYVANTGMKDSAFCNADGDFLLVPQTGRLWIETECGRLLVTPGEIAVIP 189

Query: 197 QGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKDFLAPVAWFEKSFRSGY 256
           QGFRF + LPDG SRGY+AEI+G+HFQLPDLGPIGANGLAA +DFLAP AWFE   R  Y
Sbjct: 190 QGFRFSIDLPDGKSRGYVAEIYGAHFQLPDLGPIGANGLAASRDFLAPTAWFEDGLRPEY 249

Query: 257 TIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNTVLFDHGDPSINTVLTA 316
           TIVQKFGGELFTA QDFSPFNVVAWHG+YVPYKYDL KFCPYNTVL DHGDPSINTVLTA
Sbjct: 250 TIVQKFGGELFTAKQDFSPFNVVAWHGNYVPYKYDLKKFCPYNTVLLDHGDPSINTVLTA 309

Query: 317 PTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFLPGGAS 376
           PTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYG YEAKADGFLPGGAS
Sbjct: 310 PTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGAYEAKADGFLPGGAS 369

Query: 377 LHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIPRVCAWALESPFMDRDY 436
           LH+CMTPHGPDT TYEATIAR N   P ++T TMAFMFES+LIPRVC WALESPF+D DY
Sbjct: 370 LHSCMTPHGPDTTTYEATIARVNAMAPSKLTGTMAFMFESALIPRVCHWALESPFLDHDY 429

Query: 437 YRCWIGLKSHFTNKETRDTDPKKVKTEAG 466
           Y+CWIGLKSHF+      T+ +  + E G
Sbjct: 430 YQCWIGLKSHFSRISLDKTNVESTEKEPG 458

BLAST of MC10g0912 vs. TAIR 10
Match: AT5G54080.2 (homogentisate 1,2-dioxygenase )

HSP 1 Score: 785.8 bits (2028), Expect = 1.9e-227
Identity = 356/449 (79.29%), Postives = 393/449 (87.53%), Query Frame = 0

Query: 17  DLAYQSGFNNHFSSEAIPGALPESQNSPLICPYGLYAEQISGTSFTSPRKANLRSWLYRI 76
           +L YQSGF NHFSSEAI GALP  QNSPL+CPYGLYAEQISGTSFTSPRK N RSWLYR+
Sbjct: 10  ELKYQSGFGNHFSSEAIAGALPLDQNSPLLCPYGLYAEQISGTSFTSPRKLNQRSWLYRV 69

Query: 77  KPSVTHEPFKPRLPKNQKLISEFNDSSCSSTPTQLRWKPADFPDSPVDFVDGLYTVCGAG 136
           KPSVTHEPFKPR+P ++KL+SEF+ S+  + PTQLRW+P D PDS +DFVDGL+T+CGAG
Sbjct: 70  KPSVTHEPFKPRVPAHKKLVSEFDASNSRTNPTQLRWRPEDIPDSEIDFVDGLFTICGAG 129

Query: 137 SSFLRHGFAVHMYTANKSMENCAFCNADGDFLIVPQSGRLWIITECGRLEVSPGEIVVLP 196
           SSFLRHGFA+HMY AN  M++ AFCNADGDFL+VPQ+GRLWI TECGRL V+PGEI V+P
Sbjct: 130 SSFLRHGFAIHMYVANTGMKDSAFCNADGDFLLVPQTGRLWIETECGRLLVTPGEIAVIP 189

Query: 197 QGFRFVVHLPDGPSRGYIAEIFGSHFQLPDLGPIGANGLAAPKDFLAPVAWFEKSFRSGY 256
           QGFRF + LPDG SRGY+AEI+G+HFQLPDLGPIGANGLAA +DFLAP AWFE   R  Y
Sbjct: 190 QGFRFSIDLPDGKSRGYVAEIYGAHFQLPDLGPIGANGLAASRDFLAPTAWFEDGLRPEY 249

Query: 257 TIVQKFGGELFTAIQDFSPFNVVAWHGSYVPYKYDLSKFCPYNTVLFDHGDPSINTVLTA 316
           TIVQKFGGELFTA QDFSPFNVVAWHG+YVPYKYDL KFCPYNTVL DHGDPSINTVLTA
Sbjct: 250 TIVQKFGGELFTAKQDFSPFNVVAWHGNYVPYKYDLKKFCPYNTVLLDHGDPSINTVLTA 309

Query: 317 PTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGGYEAKADGFLPGGAS 376
           PTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYG YEAKADGFLPGGAS
Sbjct: 310 PTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIYGAYEAKADGFLPGGAS 369

Query: 377 LHNCMTPHGPDTKTYEATIARGNEAGPYRITDTMAFMFESSLIPRVCAWALESPFMDRDY 436
           LH+CMTPHGPDT TYEATIAR N   P ++T TMAFMFES+LIPRVC WALESPF+D DY
Sbjct: 370 LHSCMTPHGPDTTTYEATIARVNAMAPSKLTGTMAFMFESALIPRVCHWALESPFLDHDY 429

Query: 437 YRCWIGLKSHFTNKETRDTDPKKVKTEAG 466
           Y+CWIGLKSHF+      T+ +  + E G
Sbjct: 430 YQCWIGLKSHFSRISLDKTNVESTEKEPG 458

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZRA22.7e-22679.29Homogentisate 1,2-dioxygenase OS=Arabidopsis thaliana OX=3702 GN=HGO PE=2 SV=2[more]
Q5VRH43.8e-21275.00Homogentisate 1,2-dioxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=HGO PE... [more]
Q54QI46.0e-14960.60Homogentisate 1,2-dioxygenase OS=Dictyostelium discoideum OX=44689 GN=hgd PE=2 S... [more]
O091731.8e-14556.98Homogentisate 1,2-dioxygenase OS=Mus musculus OX=10090 GN=Hgd PE=1 SV=2[more]
Q930992.6e-14456.67Homogentisate 1,2-dioxygenase OS=Homo sapiens OX=9606 GN=HGD PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_022142871.10.0100.00homogentisate 1,2-dioxygenase [Momordica charantia][more]
XP_022980282.10.091.28homogentisate 1,2-dioxygenase [Cucurbita maxima][more]
KAG6600393.10.091.06Homogentisate 1,2-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_004137214.10.090.02homogentisate 1,2-dioxygenase [Cucumis sativus][more]
XP_022942212.10.090.85homogentisate 1,2-dioxygenase [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1CNH20.0100.00Homogentisate 1,2-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111012880 PE=... [more]
A0A6J1IQY70.091.28Homogentisate 1,2-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111479699 PE=3 S... [more]
A0A0A0KYZ60.090.02Homogentisate 1,2-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_4G088750 PE=3 SV... [more]
A0A6J1FPL50.090.85Homogentisate 1,2-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111447335 PE=3... [more]
A0A5A7UWB40.089.38Homogentisate 1,2-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
Match NameE-valueIdentityDescription
AT5G54080.11.9e-22779.29homogentisate 1,2-dioxygenase [more]
AT5G54080.21.9e-22779.29homogentisate 1,2-dioxygenase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 280..418
e-value: 5.4E-63
score: 212.8
IPR005708Homogentisate 1,2-dioxygenaseTIGRFAMTIGR01015TIGR01015coord: 18..447
e-value: 6.8E-194
score: 642.4
IPR005708Homogentisate 1,2-dioxygenasePFAMPF04209HgmAcoord: 19..447
e-value: 1.4E-213
score: 709.2
IPR005708Homogentisate 1,2-dioxygenasePANTHERPTHR11056HOMOGENTISATE 1,2-DIOXYGENASEcoord: 15..450
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 451..471
NoneNo IPR availablePANTHERPTHR11056:SF0HOMOGENTISATE 1,2-DIOXYGENASEcoord: 15..450
NoneNo IPR availableCDDcd07000cupin_HGO_Ncoord: 111..219
e-value: 3.50809E-69
score: 213.924
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 17..450

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0912.1MC10g0912.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006559 L-phenylalanine catabolic process
biological_process GO:0006570 tyrosine metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005525 GTP binding
molecular_function GO:0004411 homogentisate 1,2-dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0043022 ribosome binding