MC10g0856 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g0856
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	9-cis-epoxycarotenoid dioxygenase
Location	MC10: 7267201 .. 7268961 (-)
RNA-Seq Expression	MC10g0856
Synteny	MC10g0856

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTGCTCCTTCTTCTCCATGGCTCAAATCTGGGTTCTCCCCTTCAATGCCGGAACTCCTCTCTGTAAATTCAAATTACGTCAAGAACAGGGTTTCCTGTTCTCTCCACACCCCCTCCATTGTTCAAATTCCCAAGCATTCTCAGACATTTCGCCCCCCGCCACTCAAAACCCCAGTAGAGAAACCCGTTTCTCCGTCCACGTCGGCGCCGGAGGACTGGAATTTCTTGCAGCGGGCGGCGTCCATGGCGATCGACGCTGTGGAGAATGCTCTGCTTTCGGCCGAGCGGAAGCGTCCCCTGCCCAAGACGGCCGACCCAGAAGTTCAAATTGCTGGCAATTTTGCTCCGGTGCCGGAGCAGCCCGTCCGGAAGGCTTTGCCGGTGACCGGGAGGATCCCGGAGTGCATTCGGGGGGTTTATGTTCGGAACGGCGCCAACCCGCTCCACGAACCGGCCGCCGGCCACCACTTGTTCGACGGCGATGGGATGGTCCACGCTGTGGAATTCTCCGACGGCGGCGGCGTCAGCTACGCCTGCCGGTTCACTGAGACGCAGCGGCTGGTTCAGGAGCGGGCTCATGGGCGGCCGGTTTTCCCCAAGGCCATCGGAGAACTCCACGGCCACTCCGGTATAGCGCGGCTGCTGCTCTTCTACGCCCGGGGGCTCTTTGGGCTCGTCGATCATAGCCACGGCATCGGTGTTGCCAACGCCGGGCTTGTTTACTTCAACGGCCGGCTGCTCGCCATGTCGGAGGACGATTTGCCTTACCAAATCAGGGTGACTCCGGCCGGGGATTTGAAAACGATCGGCCGGTTCGATTTTGACGGCCAGCTGAAATCCACAATGATTGCCCACCCAAAACTCGACCCTGTTTCCGGCGAGATGTTCGCTCTAAGCTACGACGTAATCCAAAAGCCCTATTTGAAATACTTCAAATTCTCGCCGGAGGGGGAGAAATCGCCGGACGTCGAGATTCCCCTGCCTCAGCCGACGATGATGCACGACTTCGCAATCACAGAGAAATTCGTCGTAATTCCCGACCAGCAGGTGGTCTTCAAGCTGCCGGAGATGATCCGCGGAGGATCTCCGGTGATCTACGACAAAAACAAAATCTCCCGATTCGGAATTTTGCCGAAAAATGCCACCGGCGCCGGCGACATTAAATGGATTGAAGCTCCCGATTGCTTCTGCTTCCACCTCTGGAATGCCTGGGAAGAACCGGAAACCAACGAAGTCGTCGTGATCGGGTCCTGCATGACGCCGCCGGACTCCATTTTCAACGAATGTGAGGAGAATCTGAAATCCGTCTTGTCGGAAATCCGCCTGAACCTCACCACCGGAAAATCGACGCGTCGGCCGATCATCCCGGAAACAGAGCAAGTGAATTTAGAAGCCGGAATGGTGAACCGAAATCGACTGGGGAGAAAAACCCAGTTCGCGTATCTTGCTCTGGCGGAGCCATGGCCGAAGGTTTCTGGATTCGCGAAGGTGGATCTGTCCACCGGAGAAATCAGAAAGTACATCTACGGCGAACAGAGGTACGGCGGCGAACCTCTGTTTCTTCCGAGAGAAGGGGCGGAGGCGGAAGACGACGGCCACATCTTGGCCTTCGTTCACGACGAGAAGGAATGGAAATCGGAGCTCCAAATCGTGAACGCCATAACTTTGAAGCTCGAAGCTTCTGTAAAGCTTCCGTCTCGAGTTCCTTATGGCTTCCATGGCACTTTCATAGGCTCCAAAGGTTTGCAGAAGCAG

mRNA sequence

ATGGCTTCTGCTCCTTCTTCTCCATGGCTCAAATCTGGGTTCTCCCCTTCAATGCCGGAACTCCTCTCTGTAAATTCAAATTACGTCAAGAACAGGGTTTCCTGTTCTCTCCACACCCCCTCCATTGTTCAAATTCCCAAGCATTCTCAGACATTTCGCCCCCCGCCACTCAAAACCCCAGTAGAGAAACCCGTTTCTCCGTCCACGTCGGCGCCGGAGGACTGGAATTTCTTGCAGCGGGCGGCGTCCATGGCGATCGACGCTGTGGAGAATGCTCTGCTTTCGGCCGAGCGGAAGCGTCCCCTGCCCAAGACGGCCGACCCAGAAGTTCAAATTGCTGGCAATTTTGCTCCGGTGCCGGAGCAGCCCGTCCGGAAGGCTTTGCCGGTGACCGGGAGGATCCCGGAGTGCATTCGGGGGGTTTATGTTCGGAACGGCGCCAACCCGCTCCACGAACCGGCCGCCGGCCACCACTTGTTCGACGGCGATGGGATGGTCCACGCTGTGGAATTCTCCGACGGCGGCGGCGTCAGCTACGCCTGCCGGTTCACTGAGACGCAGCGGCTGGTTCAGGAGCGGGCTCATGGGCGGCCGGTTTTCCCCAAGGCCATCGGAGAACTCCACGGCCACTCCGGTATAGCGCGGCTGCTGCTCTTCTACGCCCGGGGGCTCTTTGGGCTCGTCGATCATAGCCACGGCATCGGTGTTGCCAACGCCGGGCTTGTTTACTTCAACGGCCGGCTGCTCGCCATGTCGGAGGACGATTTGCCTTACCAAATCAGGGTGACTCCGGCCGGGGATTTGAAAACGATCGGCCGGTTCGATTTTGACGGCCAGCTGAAATCCACAATGATTGCCCACCCAAAACTCGACCCTGTTTCCGGCGAGATGTTCGCTCTAAGCTACGACGTAATCCAAAAGCCCTATTTGAAATACTTCAAATTCTCGCCGGAGGGGGAGAAATCGCCGGACGTCGAGATTCCCCTGCCTCAGCCGACGATGATGCACGACTTCGCAATCACAGAGAAATTCGTCGTAATTCCCGACCAGCAGGTGGTCTTCAAGCTGCCGGAGATGATCCGCGGAGGATCTCCGGTGATCTACGACAAAAACAAAATCTCCCGATTCGGAATTTTGCCGAAAAATGCCACCGGCGCCGGCGACATTAAATGGATTGAAGCTCCCGATTGCTTCTGCTTCCACCTCTGGAATGCCTGGGAAGAACCGGAAACCAACGAAGTCGTCGTGATCGGGTCCTGCATGACGCCGCCGGACTCCATTTTCAACGAATGTGAGGAGAATCTGAAATCCGTCTTGTCGGAAATCCGCCTGAACCTCACCACCGGAAAATCGACGCGTCGGCCGATCATCCCGGAAACAGAGCAAGTGAATTTAGAAGCCGGAATGGTGAACCGAAATCGACTGGGGAGAAAAACCCAGTTCGCGTATCTTGCTCTGGCGGAGCCATGGCCGAAGGTTTCTGGATTCGCGAAGGTGGATCTGTCCACCGGAGAAATCAGAAAGTACATCTACGGCGAACAGAGGTACGGCGGCGAACCTCTGTTTCTTCCGAGAGAAGGGGCGGAGGCGGAAGACGACGGCCACATCTTGGCCTTCGTTCACGACGAGAAGGAATGGAAATCGGAGCTCCAAATCGTGAACGCCATAACTTTGAAGCTCGAAGCTTCTGTAAAGCTTCCGTCTCGAGTTCCTTATGGCTTCCATGGCACTTTCATAGGCTCCAAAGGTTTGCAGAAGCAG

Coding sequence (CDS)

ATGGCTTCTGCTCCTTCTTCTCCATGGCTCAAATCTGGGTTCTCCCCTTCAATGCCGGAACTCCTCTCTGTAAATTCAAATTACGTCAAGAACAGGGTTTCCTGTTCTCTCCACACCCCCTCCATTGTTCAAATTCCCAAGCATTCTCAGACATTTCGCCCCCCGCCACTCAAAACCCCAGTAGAGAAACCCGTTTCTCCGTCCACGTCGGCGCCGGAGGACTGGAATTTCTTGCAGCGGGCGGCGTCCATGGCGATCGACGCTGTGGAGAATGCTCTGCTTTCGGCCGAGCGGAAGCGTCCCCTGCCCAAGACGGCCGACCCAGAAGTTCAAATTGCTGGCAATTTTGCTCCGGTGCCGGAGCAGCCCGTCCGGAAGGCTTTGCCGGTGACCGGGAGGATCCCGGAGTGCATTCGGGGGGTTTATGTTCGGAACGGCGCCAACCCGCTCCACGAACCGGCCGCCGGCCACCACTTGTTCGACGGCGATGGGATGGTCCACGCTGTGGAATTCTCCGACGGCGGCGGCGTCAGCTACGCCTGCCGGTTCACTGAGACGCAGCGGCTGGTTCAGGAGCGGGCTCATGGGCGGCCGGTTTTCCCCAAGGCCATCGGAGAACTCCACGGCCACTCCGGTATAGCGCGGCTGCTGCTCTTCTACGCCCGGGGGCTCTTTGGGCTCGTCGATCATAGCCACGGCATCGGTGTTGCCAACGCCGGGCTTGTTTACTTCAACGGCCGGCTGCTCGCCATGTCGGAGGACGATTTGCCTTACCAAATCAGGGTGACTCCGGCCGGGGATTTGAAAACGATCGGCCGGTTCGATTTTGACGGCCAGCTGAAATCCACAATGATTGCCCACCCAAAACTCGACCCTGTTTCCGGCGAGATGTTCGCTCTAAGCTACGACGTAATCCAAAAGCCCTATTTGAAATACTTCAAATTCTCGCCGGAGGGGGAGAAATCGCCGGACGTCGAGATTCCCCTGCCTCAGCCGACGATGATGCACGACTTCGCAATCACAGAGAAATTCGTCGTAATTCCCGACCAGCAGGTGGTCTTCAAGCTGCCGGAGATGATCCGCGGAGGATCTCCGGTGATCTACGACAAAAACAAAATCTCCCGATTCGGAATTTTGCCGAAAAATGCCACCGGCGCCGGCGACATTAAATGGATTGAAGCTCCCGATTGCTTCTGCTTCCACCTCTGGAATGCCTGGGAAGAACCGGAAACCAACGAAGTCGTCGTGATCGGGTCCTGCATGACGCCGCCGGACTCCATTTTCAACGAATGTGAGGAGAATCTGAAATCCGTCTTGTCGGAAATCCGCCTGAACCTCACCACCGGAAAATCGACGCGTCGGCCGATCATCCCGGAAACAGAGCAAGTGAATTTAGAAGCCGGAATGGTGAACCGAAATCGACTGGGGAGAAAAACCCAGTTCGCGTATCTTGCTCTGGCGGAGCCATGGCCGAAGGTTTCTGGATTCGCGAAGGTGGATCTGTCCACCGGAGAAATCAGAAAGTACATCTACGGCGAACAGAGGTACGGCGGCGAACCTCTGTTTCTTCCGAGAGAAGGGGCGGAGGCGGAAGACGACGGCCACATCTTGGCCTTCGTTCACGACGAGAAGGAATGGAAATCGGAGCTCCAAATCGTGAACGCCATAACTTTGAAGCTCGAAGCTTCTGTAAAGCTTCCGTCTCGAGTTCCTTATGGCTTCCATGGCACTTTCATAGGCTCCAAAGGTTTGCAGAAGCAG

Protein sequence

MASAPSSPWLKSGFSPSMPELLSVNSNYVKNRVSCSLHTPSIVQIPKHSQTFRPPPLKTPVEKPVSPSTSAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPVPEQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYACRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVIYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILAFVHDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ

Homology

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BLAST of MC10g0856 vs. ExPASy Swiss-Prot
Match: O24023 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED1 PE=1 SV=2)

HSP 1 Score: 881.3 bits (2276), Expect = 5.9e-255
Identity = 431/590 (73.05%), Postives = 493/590 (83.56%), Query Frame = 0


Query: 3   SAPSSPWLKSGFSPSMPELLSVNSNYVKNRVSCSLHTPSIVQIPKHSQTFRPPPLKT--- 62
           S PSS   + GF+ +   LL    N     ++ SL  P I+  PK S  ++ P   T   
Sbjct: 18  SMPSSK--EFGFASNSISLLKNQHNRQSLNINSSLQAPPILHFPKQSSNYQTPKNNTISH 77

Query: 63  PVEKPVSPSTSAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPV 122
           P ++  + S+S+   WN +Q+AA+MA+DAVE+AL   E + PLPKTADP VQI+GNFAPV
Sbjct: 78  PKQENNNSSSSSTSKWNLVQKAAAMALDAVESALTKHELEHPLPKTADPRVQISGNFAPV 137

Query: 123 PEQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSY 182
           PE PV ++LPVTG+IP+C++GVYVRNGANPL EP AGHH FDGDGMVHAV+F + G  SY
Sbjct: 138 PENPVCQSLPVTGKIPKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKN-GSASY 197

Query: 183 ACRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANA 242
           ACRFTET+RLVQE+A GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDHS G GVANA
Sbjct: 198 ACRFTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSKGTGVANA 257

Query: 243 GLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGEMFA 302
           GLVYFN RLLAMSEDDLPY ++VTP GDLKT GRFDFDGQLKSTMIAHPKLDPVSGE+FA
Sbjct: 258 GLVYFNNRLLAMSEDDLPYHVKVTPTGDLKTEGRFDFDGQLKSTMIAHPKLDPVSGELFA 317

Query: 303 LSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEM 362
           LSYDVIQKPYLKYF+FS  GEKS DVEIP+  PTMMHDFAITE FVVIPDQQVVFK+ EM
Sbjct: 318 LSYDVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITENFVVIPDQQVVFKMSEM 377

Query: 363 IRGGSPVIYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGS 422
           IRGGSPV+YDKNK+SRFGIL K A    D+KW+E PDCFCFHLWNAWEE ET+E+VVIGS
Sbjct: 378 IRGGSPVVYDKNKVSRFGILDKYAKDGSDLKWVEVPDCFCFHLWNAWEEAETDEIVVIGS 437

Query: 423 CMTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPE-TEQVNLEAGMVNRNRLGRK 482
           CMTPPDSIFNEC+E LKSVLSEIRLNL TGKSTR+ II    EQVNLEAGMVNRN+LGRK
Sbjct: 438 CMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRKSIIENPDEQVNLEAGMVNRNKLGRK 497

Query: 483 TQFAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPRE-GAEAEDDGHIL 542
           T++AYLA+AEPWPKVSGFAKV+L TGE+ K+IYG+ +YGGEPLFLPR+  ++ EDDG+IL
Sbjct: 498 TEYAYLAIAEPWPKVSGFAKVNLFTGEVEKFIYGDNKYGGEPLFLPRDPNSKEEDDGYIL 557

Query: 543 AFVHDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 588
           AFVHDEKEWKSELQIVNA++LKLEA+VKLPSRVPYGFHGTFI +  L  Q
Sbjct: 558 AFVHDEKEWKSELQIVNAMSLKLEATVKLPSRVPYGFHGTFINANDLANQ 604

[Close]

BLAST of MC10g0856 vs. ExPASy Swiss-Prot
Match: Q9M6E8 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=3885 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 864.4 bits (2232), Expect = 7.5e-250
Identity = 432/604 (71.52%), Postives = 492/604 (81.46%), Query Frame = 0


Query: 1   MASAPSSPWLKSGFSPSMPELL---SVNSNYVKNRVSCS---LHTPSIVQIPKHSQTFRP 60
           + S+ SSP+     + S P  L      S+   N ++CS   LH P   Q    S T  P
Sbjct: 14  LPSSCSSPFKDLASTSSSPTTLLPFKKRSSSNTNTITCSLQTLHYPKQYQPTSTSTTTTP 73

Query: 61  PPLK--------TP--VEKPVSPSTSA-PEDWNFLQRAASMAIDAVENALLSAERKRPLP 120
            P+K        TP    KP+S +    P+ WNFLQ+AA+  +D VE AL+S E K PLP
Sbjct: 74  TPIKPTTTTTTTTPHRETKPLSDTKQPFPQKWNFLQKAAATGLDMVETALVSHESKHPLP 133

Query: 121 KTADPEVQIAGNFAPVPEQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGD 180
           KTADP+VQIAGNFAPVPE    +ALPV G+IP+CI GVYVRNGANPL+EP AGHH FDGD
Sbjct: 134 KTADPKVQIAGNFAPVPEHAADQALPVVGKIPKCIDGVYVRNGANPLYEPVAGHHFFDGD 193

Query: 181 GMVHAVEFSDGGGVSYACRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARG 240
           GMVHAV+F++ G  SYACRFTETQRL QE++ GRPVFPKAIGELHGHSGIARLLLFYAR 
Sbjct: 194 GMVHAVKFTN-GAASYACRFTETQRLAQEKSLGRPVFPKAIGELHGHSGIARLLLFYARS 253

Query: 241 LFGLVDHSHGIGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKST 300
           LF LVD SHG+GVANAGLVYFN  LLAMSEDDLPY +R+T  GDL T+GR+DF+GQL ST
Sbjct: 254 LFQLVDGSHGMGVANAGLVYFNNHLLAMSEDDLPYHVRITSNGDLTTVGRYDFNGQLNST 313

Query: 301 MIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEK 360
           MIAHPKLDPV+G++ ALSYDV+QKPYLKYF+FS +G KSPDVEIPL +PTMMHDFAITE 
Sbjct: 314 MIAHPKLDPVNGDLHALSYDVVQKPYLKYFRFSADGVKSPDVEIPLKEPTMMHDFAITEN 373

Query: 361 FVVIPDQQVVFKLPEMIRGGSPVIYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLW 420
           FVV+PDQQVVFKL EMI GGSPV+YDKNK SRFGIL KNA  A  ++WI+AP+CFCFHLW
Sbjct: 374 FVVVPDQQVVFKLTEMITGGSPVVYDKNKTSRFGILDKNAKDANAMRWIDAPECFCFHLW 433

Query: 421 NAWEEPETNEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQV 480
           NAWEEPET+E+VVIGSCMTP DSIFNEC+E+LKSVLSEIRLNL TGKSTRRPII + EQV
Sbjct: 434 NAWEEPETDEIVVIGSCMTPADSIFNECDESLKSVLSEIRLNLRTGKSTRRPIISDAEQV 493

Query: 481 NLEAGMVNRNRLGRKTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFL 540
           NLEAGMVNRN+LGRKTQFAYLALAEPWPKVSGFAKVDL +GE++KY+YGE+++GGEPLFL
Sbjct: 494 NLEAGMVNRNKLGRKTQFAYLALAEPWPKVSGFAKVDLFSGEVQKYMYGEEKFGGEPLFL 553

Query: 541 PREGAEAEDDGHILAFVHDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKG 588
           P    E E DG+ILAFVHDEKEWKSELQIVNA  LKLEAS+KLPSRVPYGFHGTFI SK 
Sbjct: 554 P--NGEEEGDGYILAFVHDEKEWKSELQIVNAQNLKLEASIKLPSRVPYGFHGTFIHSKD 613

[Close]

BLAST of MC10g0856 vs. ExPASy Swiss-Prot
Match: K4CJJ1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 1.3e-249
Identity = 428/588 (72.79%), Postives = 488/588 (82.99%), Query Frame = 0


Query: 1   MASAPSSPWLKSGFSPSMPELLSVNSNYVKNRVSCSLHTPSIVQIPKHSQTFRPPPLKTP 60
           M S  ++  +   FSPS     S++       +S   HT +  +I     T  P    TP
Sbjct: 1   MTSTIANYRVSHSFSPSTS--YSLDFTLPSKSISMKNHTTTKTKIHSALLTL-PKQNNTP 60

Query: 61  VEKPVSPSTSAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPVP 120
             +P   ++     WNF Q+AA+ A+D VE+AL+S E + PLPKTADP VQIAGNFAPVP
Sbjct: 61  KNQPQFQTS----HWNFFQKAAAKALDIVESALVSRELQNPLPKTADPRVQIAGNFAPVP 120

Query: 121 EQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYA 180
           EQ VR  LPVTG IP+CI GVYVRNGANPL EP AGHHLFDGDGMVHAV   + G VSY+
Sbjct: 121 EQSVRHNLPVTGTIPDCINGVYVRNGANPLFEPVAGHHLFDGDGMVHAVTV-ENGSVSYS 180

Query: 181 CRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAG 240
           CRFTET+RLVQER  G PVFPKAIGELHGHSGIARLLLFYARG+FGLVDHSHG GVANAG
Sbjct: 181 CRFTETERLVQERELGHPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHSHGTGVANAG 240

Query: 241 LVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGEMFAL 300
           LV+FN RLLAMSEDD+PY ++V P+GDL+T+GR++FD QLKSTMIAHPK+DPVSGE+FAL
Sbjct: 241 LVFFNNRLLAMSEDDVPYHVQVLPSGDLQTVGRYNFDDQLKSTMIAHPKIDPVSGELFAL 300

Query: 301 SYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMI 360
           SYDV+QKPYLK FKFSP+GEKSPDVEIPL  PTMMHDFAITE +VVIPDQQVVFKL EMI
Sbjct: 301 SYDVVQKPYLKSFKFSPDGEKSPDVEIPLDVPTMMHDFAITENYVVIPDQQVVFKLQEMI 360

Query: 361 RGGSPVIYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSC 420
           +GGSPVIYDKNK SRFGILPKNA  + +I W+E+ + FCFHLWNAWEEPET+EV+VIGSC
Sbjct: 361 KGGSPVIYDKNKKSRFGILPKNAENSENIIWVESAETFCFHLWNAWEEPETDEVIVIGSC 420

Query: 421 MTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQ 480
           MTPPDSIFNEC ENLKSVLSEIRLNL TG+STRR ++  ++QVNLEAGMVNRN+LGRKTQ
Sbjct: 421 MTPPDSIFNECNENLKSVLSEIRLNLKTGESTRRQLLSPSDQVNLEAGMVNRNKLGRKTQ 480

Query: 481 FAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPRE-GAEAEDDGHILAF 540
           FAYLA+AEPWPKVSGFAKVDLSTGEI+K+IYG++RYGGEPLFLPR   +E EDDG+ILAF
Sbjct: 481 FAYLAIAEPWPKVSGFAKVDLSTGEIKKHIYGDKRYGGEPLFLPRNVNSEKEDDGYILAF 540

Query: 541 VHDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 588
            HDEK WKSELQIVNA+TL+LEA+VKLPSRVPYGFHGTFI SK LQ Q
Sbjct: 541 CHDEKTWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQNQ 580

[Close]

BLAST of MC10g0856 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 6.4e-249
Identity = 418/559 (74.78%), Postives = 478/559 (85.51%), Query Frame = 0


Query: 30  KNRVSCSLHTPSIVQIPKHSQTFRPPPLKTPVEKPVSPSTSAPEDWNFLQRAASMAIDAV 89
           K  VS +LHTP  +  PK  Q+   P +   V KP +  ++  +  N  QRAA+ A+DA 
Sbjct: 47  KLNVSSALHTPPALHFPK--QSSNSPAI---VVKPKAKESNT-KQMNLFQRAAAAALDAA 106

Query: 90  ENALLSAERKRPLPKTADPEVQIAGNFAPVPEQPVRKALPVTGRIPECIRGVYVRNGANP 149
           E  L+S E+  PLPKTADP VQIAGNFAPV EQPVR+ LPV G++P+ I+GVYVRNGANP
Sbjct: 107 EGFLVSHEKLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANP 166

Query: 150 LHEPAAGHHLFDGDGMVHAVEFSDGGGVSYACRFTETQRLVQERAHGRPVFPKAIGELHG 209
           LHEP  GHH FDGDGMVHAV+F + G  SYACRFT+T R VQER  GRPVFPKAIGELHG
Sbjct: 167 LHEPVTGHHFFDGDGMVHAVKF-EHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHG 226

Query: 210 HSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLK 269
           H+GIARL+LFYAR   G+VD +HG GVANAGLVYFNGRLLAMSEDDLPYQ+++TP GDLK
Sbjct: 227 HTGIARLMLFYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLK 286

Query: 270 TIGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPL 329
           T+GRFDFDGQL+STMIAHPK+DP SGE+FALSYDV+ KPYLKYF+FSP+G KSPDVEI L
Sbjct: 287 TVGRFDFDGQLESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQL 346

Query: 330 PQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVIYDKNKISRFGILPKNATGAGDI 389
            QPTMMHDFAITE FVV+PDQQVVFKLPEMIRGGSPV+YDKNK++RFGIL K A  + +I
Sbjct: 347 DQPTMMHDFAITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNI 406

Query: 390 KWIEAPDCFCFHLWNAWEEPETNEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLTTG 449
           KWI+APDCFCFHLWNAWEEPET+EVVVIGSCMTPPDSIFNE +ENLKSVLSEIRLNL TG
Sbjct: 407 KWIDAPDCFCFHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTG 466

Query: 450 KSTRRPIIP-ETEQVNLEAGMVNRNRLGRKTQFAYLALAEPWPKVSGFAKVDLSTGEIRK 509
           +STRRPII  E +QVNLEAGMVNRN LGRKT+FAYLALAEPWPKVSGFAKVDL+TGE++K
Sbjct: 467 ESTRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKK 526

Query: 510 YIYGEQRYGGEPLFLPREGAEAEDDGHILAFVHDEKEWKSELQIVNAITLKLEASVKLPS 569
           ++YG+ RYGGEPLFLP EG E ED+G+IL FVHDEK WKSELQIVNA++L++EA+VKLPS
Sbjct: 527 HLYGDNRYGGEPLFLPGEGGE-EDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPS 586

Query: 570 RVPYGFHGTFIGSKGLQKQ 588
           RVPYGFHGTFIG+  L KQ
Sbjct: 587 RVPYGFHGTFIGADDLAKQ 597

[Close]

BLAST of MC10g0856 vs. ExPASy Swiss-Prot
Match: Q9M9F5 (9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED9 PE=2 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 1.6e-239
Identity = 416/585 (71.11%), Postives = 480/585 (82.05%), Query Frame = 0


Query: 15  SPSMPELLSVN-SNYVKNR-------VSCSLHTPSIVQIPKHSQTFRPPPLK---TPVEK 74
           S S P+L S++ S+ ++N+       VS S++  S V     S TF+PP  K     V  
Sbjct: 74  SSSRPKLQSLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPTFKPPSWKKLCNDVTN 133

Query: 75  PVSPSTSAPEDWNFLQRAASMAIDAVENALLSAERKR-PLPKTADPEVQIAGNFAPVPEQ 134
            +  +T+     N +QR A+M +DAVENA++S ER+R P PKTADP VQIAGNF PVPE+
Sbjct: 134 LIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGNFFPVPEK 193

Query: 135 PVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYACR 194
           PV   LPVTG +PECI+GVYVRNGANPLH+P +GHHLFDGDGMVHAV F D G VSYACR
Sbjct: 194 PVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRF-DNGSVSYACR 253

Query: 195 FTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLV 254
           FTET RLVQER  GRPVFPKAIGELHGH GIA+L+LF  RGLFGLVD + G+GVANAGLV
Sbjct: 254 FTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANAGLV 313

Query: 255 YFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGEMFALSY 314
           YFNG LLAMSEDDLPY ++VT  GDL+T GR+DFDGQLKSTMIAHPK+DP + E+FALSY
Sbjct: 314 YFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDPETRELFALSY 373

Query: 315 DVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRG 374
           DV+ KPYLKYF+F+ +GEKSPDVEIPL QPTM+HDFAITE FVVIPDQQVVF+LPEMIRG
Sbjct: 374 DVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLPEMIRG 433

Query: 375 GSPVIYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSCMT 434
           GSPV+YD+ K SRFGIL KNA  A  I+WIE PDCFCFHLWN+WEEPET+EVVVIGSCMT
Sbjct: 434 GSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETDEVVVIGSCMT 493

Query: 435 PPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQFA 494
           PPDSIFNE +E L+SVLSEIRLNL TG+STRRP+I  +EQVNLEAGMVNRN LGRKT++A
Sbjct: 494 PPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVI--SEQVNLEAGMVNRNLLGRKTRYA 553

Query: 495 YLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILAFVHD 554
           YLAL EPWPKVSGFAKVDLSTGEIRKYIYGE +YGGEPLFLP  G   ED G+I+ FVHD
Sbjct: 554 YLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP-SGDGEEDGGYIMVFVHD 613

Query: 555 EKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 588
           E++ KSELQ++NA+ +KLEA+V LPSRVPYGFHGTFI  + L KQ
Sbjct: 614 EEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQ 654

[Close]

BLAST of MC10g0856 vs. NCBI nr
Match: AFU91491.1 (9-cis-epoxycarotenoid dioxygenase [Momordica charantia])

HSP 1 Score: 1183 bits (3061), Expect = 0.0
Identity = 582/587 (99.15%), Postives = 586/587 (99.83%), Query Frame = 0


Query: 1   MASAPSSPWLKSGFSPSMPELLSVNSNYVKNRVSCSLHTPSIVQIPKHSQTFRPPPLKTP 60
           MASAPSSPWLKSGFSPSMPELLSVNSNYVKNRVSCSLHTPSIVQIPKHSQTFRPPPLKTP
Sbjct: 1   MASAPSSPWLKSGFSPSMPELLSVNSNYVKNRVSCSLHTPSIVQIPKHSQTFRPPPLKTP 60

Query: 61  VEKPVSPSTSAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPVP 120
           VEKPVSPSTSAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPVP
Sbjct: 61  VEKPVSPSTSAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPVP 120

Query: 121 EQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYA 180
           EQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYA
Sbjct: 121 EQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYA 180

Query: 181 CRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAG 240
           CRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAG
Sbjct: 181 CRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAG 240

Query: 241 LVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGEMFAL 300
           LVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGE+FA+
Sbjct: 241 LVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGELFAI 300

Query: 301 SYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMI 360
           SYDVIQKP+LKYFKFSPEGEKSPDVE+PLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMI
Sbjct: 301 SYDVIQKPHLKYFKFSPEGEKSPDVEVPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMI 360

Query: 361 RGGSPVIYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSC 420
           RGGSPVIYDKNKISRFGILPKNAT AGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSC
Sbjct: 361 RGGSPVIYDKNKISRFGILPKNATDAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSC 420

Query: 421 MTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQ 480
           MTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQ
Sbjct: 421 MTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQ 480

Query: 481 FAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILAFV 540
           FAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILAFV
Sbjct: 481 FAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILAFV 540

Query: 541 HDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587
           HDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ
Sbjct: 541 HDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587

[Close]

BLAST of MC10g0856 vs. NCBI nr
Match: APO14273.1 (9-cis-epoxycarotenoid dioxygenase NCED1 [Luffa aegyptiaca])

HSP 1 Score: 1059 bits (2738), Expect = 0.0
Identity = 521/587 (88.76%), Postives = 554/587 (94.38%), Query Frame = 0


Query: 6   SSPWLKSGFSPSMPELLSVNSNY-VKNRVSCSLHTPSIVQIPKHSQTFRPPPLKTPVEKP 65
           SS W+KS FS SMPEL+SVN NY ++NRV+CSLHTPSI+QIPKHSQT    P+KT V KP
Sbjct: 8   SSSWVKSRFSSSMPELVSVNRNYGLQNRVACSLHTPSIIQIPKHSQTAFRSPVKTAVPKP 67

Query: 66  VSPS----TSAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPVP 125
           V  S    +S  E+WNFLQRAA++A+DAVE+AL+SAERK  LPKTADP VQIAGNFAPVP
Sbjct: 68  VPVSPPSVSSGSEEWNFLQRAAAVALDAVEDALVSAERKHSLPKTADPAVQIAGNFAPVP 127

Query: 126 EQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYA 185
           EQPVRKALPVTG++P+CIRGVYVRNGANPLHEP +GHHLFDGDGMVHAVEFS+GGGVSYA
Sbjct: 128 EQPVRKALPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGGVSYA 187

Query: 186 CRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAG 245
           CRFTETQRLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDHSHG+GVANAG
Sbjct: 188 CRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGLGVANAG 247

Query: 246 LVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGEMFAL 305
           LVYFNGRLLAMSEDDLPYQIRVTPAGDLKT+GRFDFDGQLKSTMIAHPKLDPVSGEMFAL
Sbjct: 248 LVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFAL 307

Query: 306 SYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMI 365
           SYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMI
Sbjct: 308 SYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMI 367

Query: 366 RGGSPVIYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSC 425
           RGGSPV+YDK K SRFGIL KNAT A  IKWIEAPDCFCFHLWNAWEEPET+E+VVIGSC
Sbjct: 368 RGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETDEIVVIGSC 427

Query: 426 MTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQ 485
           MTPPDSIFNECEENLKSVLSEIRLNL+TGKSTRRPII ETEQVNLEAGMVNRN+LGRKTQ
Sbjct: 428 MTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQ 487

Query: 486 FAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILAFV 545
           FAYLALAEPWPKVSGFAKVDL+TGEIRKY+YGEQRYGGEPLFLPREGA AEDDGHILAFV
Sbjct: 488 FAYLALAEPWPKVSGFAKVDLATGEIRKYLYGEQRYGGEPLFLPREGATAEDDGHILAFV 547

Query: 546 HDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587
           HDEKEWKSELQIVNA+TL+LEA+VKLPSRVPYGFHGTFI SK LQKQ
Sbjct: 548 HDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 594

[Close]

BLAST of MC10g0856 vs. NCBI nr
Match: XP_038878047.1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic [Benincasa hispida])

HSP 1 Score: 1037 bits (2682), Expect = 0.0
Identity = 517/589 (87.78%), Postives = 546/589 (92.70%), Query Frame = 0


Query: 2   ASAPSSPWLKSGFSPSMPELLSVNSNYVKNRVS--CSLHTPSIVQIPKHSQTFRPPPLKT 61
           +S+ SS  LKS  S  M + LS+NSNY +NRV+  CSLHTPSI+QIPKHS T  P PLKT
Sbjct: 4   SSSSSSSLLKSPLSSPMLQNLSLNSNYGRNRVAPTCSLHTPSIIQIPKHSHTAFPSPLKT 63

Query: 62  PVEKPVSPSTSAPE-DWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAP 121
            V  P  PS S+P  DWNFLQRAA++A+DAVENAL+SAERK  LPKTADP VQIAGNFAP
Sbjct: 64  TVP-PSPPSLSSPSHDWNFLQRAAAIALDAVENALVSAERKHSLPKTADPAVQIAGNFAP 123

Query: 122 VPEQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVS 181
           VPEQPVRK LPVTG++P+ IRGVYVRNGANPLHEP +GHHLFDGDGMVHAVEFSDGG VS
Sbjct: 124 VPEQPVRKGLPVTGKVPDSIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSDGGAVS 183

Query: 182 YACRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVAN 241
           YACRFTETQRLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+HGIGVAN
Sbjct: 184 YACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVAN 243

Query: 242 AGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGEMF 301
           AGLV+FNGRLLAMSEDDLPYQI VTPAGDLKT+GRFDF GQL STMIAHPKLDPVSGEMF
Sbjct: 244 AGLVFFNGRLLAMSEDDLPYQITVTPAGDLKTVGRFDFHGQLTSTMIAHPKLDPVSGEMF 303

Query: 302 ALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPE 361
           ALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPE
Sbjct: 304 ALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPE 363

Query: 362 MIRGGSPVIYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIG 421
           MIRGGSPV+YDK KISRFGIL KNAT A  IKWIEAPDCFCFHLWNAWEEPETNE+VVIG
Sbjct: 364 MIRGGSPVVYDKEKISRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIG 423

Query: 422 SCMTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRK 481
           SCMTPPDSIFNECEENLKSVLSEIRLNL+TGKSTRRPII ETEQVNLEAGMVNRNRLGRK
Sbjct: 424 SCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNRLGRK 483

Query: 482 TQFAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILA 541
           TQFAYLALAEPWPKVSGFAKVDL TGEIRKY+YG+QRYGGEPLFLPREGAEAEDDGHILA
Sbjct: 484 TQFAYLALAEPWPKVSGFAKVDLFTGEIRKYLYGDQRYGGEPLFLPREGAEAEDDGHILA 543

Query: 542 FVHDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587
           FVHDEKEWKSELQIVNA+TL+LEA+VKLPSRVPYGFHGTFI SK LQKQ
Sbjct: 544 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 591

[Close]

BLAST of MC10g0856 vs. NCBI nr
Match: XP_008465499.1 (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1031 bits (2667), Expect = 0.0
Identity = 513/589 (87.10%), Postives = 540/589 (91.68%), Query Frame = 0


Query: 6   SSPWLKSGFSPSMPELLSVNSNYVKNR---VSCSLHTPSIVQIPKHSQTFRPPPLKTPVE 65
           SS +LKS FS SMP+     SNY   R   VS SLHTPSI+QIPKHS T  P PLKT + 
Sbjct: 3   SSSFLKSPFSSSMPD--KTLSNYTSRRLLSVSSSLHTPSIIQIPKHSHTTFPSPLKTSIP 62

Query: 66  KPVSPSTSAP----EDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAP 125
           KP   ST+ P    EDWNFLQRAA+MA+DAVENAL+SAERK  LPKTADP VQIAGNFAP
Sbjct: 63  KPPPLSTTPPLSGSEDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFAP 122

Query: 126 VPEQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVS 185
           VPEQPVRK LPV G++PECIRGVYVRNGANPLHEP +GHHLFDGDGMVHAVEFS+ GGVS
Sbjct: 123 VPEQPVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEAGGVS 182

Query: 186 YACRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVAN 245
           YACRFTETQRLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+ GIGVAN
Sbjct: 183 YACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNQGIGVAN 242

Query: 246 AGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGEMF 305
           AGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKT+GRFDFDGQLKSTMIAHPKLDPVSGEMF
Sbjct: 243 AGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMF 302

Query: 306 ALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPE 365
           ALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEK+VVIPDQQVVFKLPE
Sbjct: 303 ALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLPE 362

Query: 366 MIRGGSPVIYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIG 425
           MIRGGSPV+YDK K SRFGIL KNA  A  IKWIEAPDCFCFHLWNAWEEPETNE+VVIG
Sbjct: 363 MIRGGSPVVYDKEKTSRFGILDKNAADANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIG 422

Query: 426 SCMTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRK 485
           SCMTPPDSIFNECEENLKSVLSEIRLNL+TGKSTRRPII ETEQVNLEAGMVNRN LGRK
Sbjct: 423 SCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIISETEQVNLEAGMVNRNLLGRK 482

Query: 486 TQFAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILA 545
           TQF+YLALAEPWPKVSGFAKVDL TGEI+KY+YGEQRYGGEPLFLPREGAEAEDDGHILA
Sbjct: 483 TQFSYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHILA 542

Query: 546 FVHDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587
           FVHDEKEWKSELQIVNA+TL+LEA+VKLPSRVPYGFHGTFI S+ LQKQ
Sbjct: 543 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQ 589

[Close]

BLAST of MC10g0856 vs. NCBI nr
Match: XP_023529573.1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1030 bits (2663), Expect = 0.0
Identity = 508/581 (87.44%), Postives = 542/581 (93.29%), Query Frame = 0


Query: 12  SGFSPSMPELLSVNSNY-VKNRVSCSLHTPSIVQIPKHSQTFRPPPLKTPVEKP---VSP 71
           S  S SMP++LSVN NY V+NRVSCSLHTPSI+QIPK SQT    P+K  V KP    +P
Sbjct: 4   SSSSSSMPKILSVNRNYGVRNRVSCSLHTPSIIQIPKRSQTAFRSPIKIAVPKPEPVAAP 63

Query: 72  STSAP-EDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPVPEQPVRK 131
           S S+P ++WNFLQ+AA+MA+DAVE+AL+S E+K  LPKTADP VQIAGNFAPVPEQ VRK
Sbjct: 64  SVSSPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFAPVPEQSVRK 123

Query: 132 ALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYACRFTET 191
            LPVTG++P+CIRGVYVRNGANPLHEP +GHHLFDGDGMVHAVEF++GG VSYACRFTET
Sbjct: 124 VLPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEGGRVSYACRFTET 183

Query: 192 QRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNG 251
           +RLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDHSHGIGVANAGLVYFNG
Sbjct: 184 ERLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVANAGLVYFNG 243

Query: 252 RLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQ 311
           RLLAMSEDDLPYQIRVTP+GDLKT+GRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQ
Sbjct: 244 RLLAMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQ 303

Query: 312 KPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPV 371
           KPYLKYFKFS EGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPV
Sbjct: 304 KPYLKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPV 363

Query: 372 IYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSCMTPPDS 431
           +YDK K SRFGIL KNAT A  IKWIEAPDCFCFHLWNAWEEPETNE+VVIGSCMTPPDS
Sbjct: 364 VYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDS 423

Query: 432 IFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQFAYLAL 491
           IFNECEENLKSVLSEIRLNL+TGKSTRRPII ETEQVNLEAGMVNRN+LGRKTQFAYLAL
Sbjct: 424 IFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFAYLAL 483

Query: 492 AEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILAFVHDEKEW 551
           AEPWPKVSGFAKVDLSTGEIRKY+YGE RYGGEPLFLPREGA AEDDGHILAFVHDEKEW
Sbjct: 484 AEPWPKVSGFAKVDLSTGEIRKYLYGEDRYGGEPLFLPREGATAEDDGHILAFVHDEKEW 543

Query: 552 KSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587
           KSELQIVNA+TL+LEA+VKLPSRVPYGFHGTFI SK LQKQ
Sbjct: 544 KSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 584

[Close]

BLAST of MC10g0856 vs. ExPASy TrEMBL
Match: K4KBB8 (9-cis-epoxycarotenoid dioxygenase OS=Momordica charantia OX=3673 GN=NCED PE=2 SV=1)

HSP 1 Score: 1183 bits (3061), Expect = 0.0
Identity = 582/587 (99.15%), Postives = 586/587 (99.83%), Query Frame = 0


Query: 1   MASAPSSPWLKSGFSPSMPELLSVNSNYVKNRVSCSLHTPSIVQIPKHSQTFRPPPLKTP 60
           MASAPSSPWLKSGFSPSMPELLSVNSNYVKNRVSCSLHTPSIVQIPKHSQTFRPPPLKTP
Sbjct: 1   MASAPSSPWLKSGFSPSMPELLSVNSNYVKNRVSCSLHTPSIVQIPKHSQTFRPPPLKTP 60

Query: 61  VEKPVSPSTSAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPVP 120
           VEKPVSPSTSAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPVP
Sbjct: 61  VEKPVSPSTSAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPVP 120

Query: 121 EQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYA 180
           EQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYA
Sbjct: 121 EQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYA 180

Query: 181 CRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAG 240
           CRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAG
Sbjct: 181 CRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAG 240

Query: 241 LVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGEMFAL 300
           LVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGE+FA+
Sbjct: 241 LVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGELFAI 300

Query: 301 SYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMI 360
           SYDVIQKP+LKYFKFSPEGEKSPDVE+PLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMI
Sbjct: 301 SYDVIQKPHLKYFKFSPEGEKSPDVEVPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMI 360

Query: 361 RGGSPVIYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSC 420
           RGGSPVIYDKNKISRFGILPKNAT AGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSC
Sbjct: 361 RGGSPVIYDKNKISRFGILPKNATDAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSC 420

Query: 421 MTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQ 480
           MTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQ
Sbjct: 421 MTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQ 480

Query: 481 FAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILAFV 540
           FAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILAFV
Sbjct: 481 FAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILAFV 540

Query: 541 HDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587
           HDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ
Sbjct: 541 HDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587

[Close]

BLAST of MC10g0856 vs. ExPASy TrEMBL
Match: A0A1L5JHV0 (9-cis-epoxycarotenoid dioxygenase NCED1 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 1059 bits (2738), Expect = 0.0
Identity = 521/587 (88.76%), Postives = 554/587 (94.38%), Query Frame = 0


Query: 6   SSPWLKSGFSPSMPELLSVNSNY-VKNRVSCSLHTPSIVQIPKHSQTFRPPPLKTPVEKP 65
           SS W+KS FS SMPEL+SVN NY ++NRV+CSLHTPSI+QIPKHSQT    P+KT V KP
Sbjct: 8   SSSWVKSRFSSSMPELVSVNRNYGLQNRVACSLHTPSIIQIPKHSQTAFRSPVKTAVPKP 67

Query: 66  VSPS----TSAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPVP 125
           V  S    +S  E+WNFLQRAA++A+DAVE+AL+SAERK  LPKTADP VQIAGNFAPVP
Sbjct: 68  VPVSPPSVSSGSEEWNFLQRAAAVALDAVEDALVSAERKHSLPKTADPAVQIAGNFAPVP 127

Query: 126 EQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYA 185
           EQPVRKALPVTG++P+CIRGVYVRNGANPLHEP +GHHLFDGDGMVHAVEFS+GGGVSYA
Sbjct: 128 EQPVRKALPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEGGGVSYA 187

Query: 186 CRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAG 245
           CRFTETQRLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDHSHG+GVANAG
Sbjct: 188 CRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGLGVANAG 247

Query: 246 LVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGEMFAL 305
           LVYFNGRLLAMSEDDLPYQIRVTPAGDLKT+GRFDFDGQLKSTMIAHPKLDPVSGEMFAL
Sbjct: 248 LVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFAL 307

Query: 306 SYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMI 365
           SYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMI
Sbjct: 308 SYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMI 367

Query: 366 RGGSPVIYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSC 425
           RGGSPV+YDK K SRFGIL KNAT A  IKWIEAPDCFCFHLWNAWEEPET+E+VVIGSC
Sbjct: 368 RGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETDEIVVIGSC 427

Query: 426 MTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQ 485
           MTPPDSIFNECEENLKSVLSEIRLNL+TGKSTRRPII ETEQVNLEAGMVNRN+LGRKTQ
Sbjct: 428 MTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQ 487

Query: 486 FAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILAFV 545
           FAYLALAEPWPKVSGFAKVDL+TGEIRKY+YGEQRYGGEPLFLPREGA AEDDGHILAFV
Sbjct: 488 FAYLALAEPWPKVSGFAKVDLATGEIRKYLYGEQRYGGEPLFLPREGATAEDDGHILAFV 547

Query: 546 HDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587
           HDEKEWKSELQIVNA+TL+LEA+VKLPSRVPYGFHGTFI SK LQKQ
Sbjct: 548 HDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 594

[Close]

BLAST of MC10g0856 vs. ExPASy TrEMBL
Match: A0A1S3CQH4 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103503120 PE=3 SV=1)

HSP 1 Score: 1031 bits (2667), Expect = 0.0
Identity = 513/589 (87.10%), Postives = 540/589 (91.68%), Query Frame = 0


Query: 6   SSPWLKSGFSPSMPELLSVNSNYVKNR---VSCSLHTPSIVQIPKHSQTFRPPPLKTPVE 65
           SS +LKS FS SMP+     SNY   R   VS SLHTPSI+QIPKHS T  P PLKT + 
Sbjct: 3   SSSFLKSPFSSSMPD--KTLSNYTSRRLLSVSSSLHTPSIIQIPKHSHTTFPSPLKTSIP 62

Query: 66  KPVSPSTSAP----EDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAP 125
           KP   ST+ P    EDWNFLQRAA+MA+DAVENAL+SAERK  LPKTADP VQIAGNFAP
Sbjct: 63  KPPPLSTTPPLSGSEDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFAP 122

Query: 126 VPEQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVS 185
           VPEQPVRK LPV G++PECIRGVYVRNGANPLHEP +GHHLFDGDGMVHAVEFS+ GGVS
Sbjct: 123 VPEQPVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEAGGVS 182

Query: 186 YACRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVAN 245
           YACRFTETQRLVQERA+GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+ GIGVAN
Sbjct: 183 YACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNQGIGVAN 242

Query: 246 AGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGEMF 305
           AGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKT+GRFDFDGQLKSTMIAHPKLDPVSGEMF
Sbjct: 243 AGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMF 302

Query: 306 ALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPE 365
           ALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEK+VVIPDQQVVFKLPE
Sbjct: 303 ALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLPE 362

Query: 366 MIRGGSPVIYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIG 425
           MIRGGSPV+YDK K SRFGIL KNA  A  IKWIEAPDCFCFHLWNAWEEPETNE+VVIG
Sbjct: 363 MIRGGSPVVYDKEKTSRFGILDKNAADANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIG 422

Query: 426 SCMTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRK 485
           SCMTPPDSIFNECEENLKSVLSEIRLNL+TGKSTRRPII ETEQVNLEAGMVNRN LGRK
Sbjct: 423 SCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIISETEQVNLEAGMVNRNLLGRK 482

Query: 486 TQFAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILA 545
           TQF+YLALAEPWPKVSGFAKVDL TGEI+KY+YGEQRYGGEPLFLPREGAEAEDDGHILA
Sbjct: 483 TQFSYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHILA 542

Query: 546 FVHDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587
           FVHDEKEWKSELQIVNA+TL+LEA+VKLPSRVPYGFHGTFI S+ LQKQ
Sbjct: 543 FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQ 589

[Close]

BLAST of MC10g0856 vs. ExPASy TrEMBL
Match: A0A6J1ETU1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111437497 PE=3 SV=1)

HSP 1 Score: 1026 bits (2653), Expect = 0.0
Identity = 502/588 (85.37%), Postives = 539/588 (91.67%), Query Frame = 0


Query: 1   MASAPSSPWLKSGFSPSMPELLSVNSNYVKNRVSCSLHTPSIVQIPKHSQTFRPPPLKTP 60
           + S+ SS W+KSGFS SM E  SVN +  +NRV+CSLHTPSI+QIPK SQT    P+KT 
Sbjct: 4   LVSSSSSSWVKSGFSSSMSECFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTT 63

Query: 61  VEKPVSPST-SAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPV 120
           V KPV  S  S  E+WNFLQRAA+MA+DAVE+AL+SAE+K  LPKTADP VQIAGNFAPV
Sbjct: 64  VPKPVPVSAPSVSEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAGNFAPV 123

Query: 121 PEQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSY 180
           PEQ VRK LPV G++PECIRGVYVRNGANPLHEP +GHHLFDGDGMVHAVEF++ GGVSY
Sbjct: 124 PEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGGVSY 183

Query: 181 ACRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANA 240
           ACRFTETQRLVQERA+GRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANA
Sbjct: 184 ACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANA 243

Query: 241 GLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGEMFA 300
           GLVYFNG+LLAMSEDDLPY+IRVTPAGDLKT+GRFDFDGQLKSTMIAHPKLDPVSGEMFA
Sbjct: 244 GLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFA 303

Query: 301 LSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEM 360
           LSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFV+IPDQQVVFKLPEM
Sbjct: 304 LSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEM 363

Query: 361 IRGGSPVIYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGS 420
           IRGGSPVIYDK K SRFG++ KNAT A  IKWI+ PDCFCFHLWNAWEEPET E+VVIGS
Sbjct: 364 IRGGSPVIYDKEKTSRFGVMDKNATDADAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGS 423

Query: 421 CMTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKT 480
           CMTPPDSIFNECEENL S+LSEIRLNL+TGKSTRRPII ETEQVNLEAGMVNRN+LGRKT
Sbjct: 424 CMTPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKT 483

Query: 481 QFAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILAF 540
           QF Y+ALAEPWPKVSGF KV+L TGE RKY+YGEQRYGGEPLFLPREGAEAEDDGHIL F
Sbjct: 484 QFVYVALAEPWPKVSGFVKVNLFTGESRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTF 543

Query: 541 VHDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587
           VHDEKEWKSELQIVNA+TL+LEA+VKLPSRVPYGFHGTFI S  LQKQ
Sbjct: 544 VHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 591

[Close]

BLAST of MC10g0856 vs. ExPASy TrEMBL
Match: A0A6J1FPE2 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111447037 PE=3 SV=1)

HSP 1 Score: 1025 bits (2651), Expect = 0.0
Identity = 505/578 (87.37%), Postives = 540/578 (93.43%), Query Frame = 0


Query: 15  SPSMPELLSVNSNY-VKNRVSCSLHTPSIVQIPKHSQTFRPPPLKTPVEKP---VSPSTS 74
           S SMP++LSVN NY V+NRVSCSLHTPSI+QIPK SQT    P+K  V KP    +PS S
Sbjct: 4   SSSMPKILSVNRNYGVRNRVSCSLHTPSIIQIPKRSQTAFRSPIKIAVPKPEPVAAPSVS 63

Query: 75  AP-EDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPVPEQPVRKALP 134
           +P ++WNFLQ+AA+MA+DAVE+AL+S E+K  LPKTADP VQIAGNFAPVPEQPVRK LP
Sbjct: 64  SPAKEWNFLQQAAAMALDAVEDALVSMEKKHSLPKTADPSVQIAGNFAPVPEQPVRKVLP 123

Query: 135 VTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYACRFTETQRL 194
           VTG++P+CIRGVYVRNGANPLHEP +GHHLFDGDGMVHAVEF++ G VSYACRFTET+RL
Sbjct: 124 VTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTECGRVSYACRFTETERL 183

Query: 195 VQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGRLL 254
           VQERA+GRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDHSHGIGVANAGLVYFNGRLL
Sbjct: 184 VQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSHGIGVANAGLVYFNGRLL 243

Query: 255 AMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPY 314
           AMSEDDLPYQIRVTP+GDLKT+GRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPY
Sbjct: 244 AMSEDDLPYQIRVTPSGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPY 303

Query: 315 LKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVIYD 374
           LKYFKFS EGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPV+YD
Sbjct: 304 LKYFKFSAEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVVYD 363

Query: 375 KNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSCMTPPDSIFN 434
           K K SRFGIL KNAT A  IKWIEAPDCFCFHLWNAWEEPE+NE+VVIGSCMTPPDSIFN
Sbjct: 364 KEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPESNEIVVIGSCMTPPDSIFN 423

Query: 435 ECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQFAYLALAEP 494
           ECEENLKSVLSEIRLNL+TGKSTRRPII ETEQVNLEAGMVNRN+LGRKTQFAYLALAEP
Sbjct: 424 ECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQLGRKTQFAYLALAEP 483

Query: 495 WPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILAFVHDEKEWKSE 554
           WPKVSGFAKVDLSTGEIRKY+YG  RYGGEPLFLPREGA AEDDGHILAFVHDEKEWKSE
Sbjct: 484 WPKVSGFAKVDLSTGEIRKYLYGADRYGGEPLFLPREGATAEDDGHILAFVHDEKEWKSE 543

Query: 555 LQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 587
           LQIVNA+TL+LEA+VKLPSRVPYGFHGTFI SK LQKQ
Sbjct: 544 LQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQ 581

[Close]

BLAST of MC10g0856 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 861.3 bits (2224), Expect = 4.5e-250
Identity = 418/559 (74.78%), Postives = 478/559 (85.51%), Query Frame = 0


Query: 30  KNRVSCSLHTPSIVQIPKHSQTFRPPPLKTPVEKPVSPSTSAPEDWNFLQRAASMAIDAV 89
           K  VS +LHTP  +  PK  Q+   P +   V KP +  ++  +  N  QRAA+ A+DA 
Sbjct: 47  KLNVSSALHTPPALHFPK--QSSNSPAI---VVKPKAKESNT-KQMNLFQRAAAAALDAA 106

Query: 90  ENALLSAERKRPLPKTADPEVQIAGNFAPVPEQPVRKALPVTGRIPECIRGVYVRNGANP 149
           E  L+S E+  PLPKTADP VQIAGNFAPV EQPVR+ LPV G++P+ I+GVYVRNGANP
Sbjct: 107 EGFLVSHEKLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANP 166

Query: 150 LHEPAAGHHLFDGDGMVHAVEFSDGGGVSYACRFTETQRLVQERAHGRPVFPKAIGELHG 209
           LHEP  GHH FDGDGMVHAV+F + G  SYACRFT+T R VQER  GRPVFPKAIGELHG
Sbjct: 167 LHEPVTGHHFFDGDGMVHAVKF-EHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHG 226

Query: 210 HSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLK 269
           H+GIARL+LFYAR   G+VD +HG GVANAGLVYFNGRLLAMSEDDLPYQ+++TP GDLK
Sbjct: 227 HTGIARLMLFYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLK 286

Query: 270 TIGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPL 329
           T+GRFDFDGQL+STMIAHPK+DP SGE+FALSYDV+ KPYLKYF+FSP+G KSPDVEI L
Sbjct: 287 TVGRFDFDGQLESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQL 346

Query: 330 PQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVIYDKNKISRFGILPKNATGAGDI 389
            QPTMMHDFAITE FVV+PDQQVVFKLPEMIRGGSPV+YDKNK++RFGIL K A  + +I
Sbjct: 347 DQPTMMHDFAITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNI 406

Query: 390 KWIEAPDCFCFHLWNAWEEPETNEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLTTG 449
           KWI+APDCFCFHLWNAWEEPET+EVVVIGSCMTPPDSIFNE +ENLKSVLSEIRLNL TG
Sbjct: 407 KWIDAPDCFCFHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTG 466

Query: 450 KSTRRPIIP-ETEQVNLEAGMVNRNRLGRKTQFAYLALAEPWPKVSGFAKVDLSTGEIRK 509
           +STRRPII  E +QVNLEAGMVNRN LGRKT+FAYLALAEPWPKVSGFAKVDL+TGE++K
Sbjct: 467 ESTRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKK 526

Query: 510 YIYGEQRYGGEPLFLPREGAEAEDDGHILAFVHDEKEWKSELQIVNAITLKLEASVKLPS 569
           ++YG+ RYGGEPLFLP EG E ED+G+IL FVHDEK WKSELQIVNA++L++EA+VKLPS
Sbjct: 527 HLYGDNRYGGEPLFLPGEGGE-EDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPS 586

Query: 570 RVPYGFHGTFIGSKGLQKQ 588
           RVPYGFHGTFIG+  L KQ
Sbjct: 587 RVPYGFHGTFIGADDLAKQ 597

[Close]

BLAST of MC10g0856 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 830.1 bits (2143), Expect = 1.1e-240
Identity = 416/585 (71.11%), Postives = 480/585 (82.05%), Query Frame = 0


Query: 15  SPSMPELLSVN-SNYVKNR-------VSCSLHTPSIVQIPKHSQTFRPPPLK---TPVEK 74
           S S P+L S++ S+ ++N+       VS S++  S V     S TF+PP  K     V  
Sbjct: 74  SSSRPKLQSLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPTFKPPSWKKLCNDVTN 133

Query: 75  PVSPSTSAPEDWNFLQRAASMAIDAVENALLSAERKR-PLPKTADPEVQIAGNFAPVPEQ 134
            +  +T+     N +QR A+M +DAVENA++S ER+R P PKTADP VQIAGNF PVPE+
Sbjct: 134 LIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGNFFPVPEK 193

Query: 135 PVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYACR 194
           PV   LPVTG +PECI+GVYVRNGANPLH+P +GHHLFDGDGMVHAV F D G VSYACR
Sbjct: 194 PVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRF-DNGSVSYACR 253

Query: 195 FTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLV 254
           FTET RLVQER  GRPVFPKAIGELHGH GIA+L+LF  RGLFGLVD + G+GVANAGLV
Sbjct: 254 FTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANAGLV 313

Query: 255 YFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGEMFALSY 314
           YFNG LLAMSEDDLPY ++VT  GDL+T GR+DFDGQLKSTMIAHPK+DP + E+FALSY
Sbjct: 314 YFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDPETRELFALSY 373

Query: 315 DVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRG 374
           DV+ KPYLKYF+F+ +GEKSPDVEIPL QPTM+HDFAITE FVVIPDQQVVF+LPEMIRG
Sbjct: 374 DVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLPEMIRG 433

Query: 375 GSPVIYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSCMT 434
           GSPV+YD+ K SRFGIL KNA  A  I+WIE PDCFCFHLWN+WEEPET+EVVVIGSCMT
Sbjct: 434 GSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETDEVVVIGSCMT 493

Query: 435 PPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQFA 494
           PPDSIFNE +E L+SVLSEIRLNL TG+STRRP+I  +EQVNLEAGMVNRN LGRKT++A
Sbjct: 494 PPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVI--SEQVNLEAGMVNRNLLGRKTRYA 553

Query: 495 YLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAEAEDDGHILAFVHD 554
           YLAL EPWPKVSGFAKVDLSTGEIRKYIYGE +YGGEPLFLP  G   ED G+I+ FVHD
Sbjct: 554 YLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP-SGDGEEDGGYIMVFVHD 613

Query: 555 EKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 588
           E++ KSELQ++NA+ +KLEA+V LPSRVPYGFHGTFI  + L KQ
Sbjct: 614 EEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQ 654

[Close]

BLAST of MC10g0856 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 810.1 bits (2091), Expect = 1.2e-234
Identity = 394/582 (67.70%), Postives = 465/582 (79.90%), Query Frame = 0


Query: 7   SPWLKSGFSPSMPELLSVNSNYVKNRVSCSLHTPSIVQIPKHSQTFRPPPLKTPVEKPVS 66
           SP     F+ + P    +++N V +       TP+++  P +           P   P+ 
Sbjct: 27  SPSSSVSFTNTKPRRRKLSANSVSD-------TPNLLNFPNY-----------PSPNPII 86

Query: 67  PSTSAPEDWNFLQRAASMAIDAVENALLSAERKRPLPKTADPEVQIAGNFAPVPEQPVRK 126
           P       WN LQRAAS A+D  E ALL  ER +PLPKT DP  QI+GN+APVPEQ V+ 
Sbjct: 87  PEKDTSR-WNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKS 146

Query: 127 ALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSDGGGVSYACRFTET 186
           +L V G+IP+CI GVY+RNGANPL EP +GHHLFDGDGMVHAV+ ++ G  SY+CRFTET
Sbjct: 147 SLSVDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITN-GDASYSCRFTET 206

Query: 187 QRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNG 246
           +RLVQE+  G P+FPKAIGELHGHSGIARL+LFYARGLFGL++H +G GVANAGLVYF+ 
Sbjct: 207 ERLVQEKQLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHD 266

Query: 247 RLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQ 306
           RLLAMSEDDLPYQ+RVT  GDL+TIGRFDFDGQL S MIAHPK+DPV+ E+FALSYDV++
Sbjct: 267 RLLAMSEDDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVK 326

Query: 307 KPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPV 366
           KPYLKYFKFSPEGEKSPDVEIPL  PTMMHDFAITE FVVIPDQQVVFKL +M  G SPV
Sbjct: 327 KPYLKYFKFSPEGEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPV 386

Query: 367 IYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNEVVVIGSCMTPPDS 426
            YD  KISRFGILP+NA  A ++ W+E+P+ FCFHLWNAWE PET+EVVVIGSCMTP DS
Sbjct: 387 KYDGEKISRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADS 446

Query: 427 IFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGMVNRNRLGRKTQFAYLAL 486
           IFNEC+E L SVLSEIRLNL TGKSTRR IIP + Q+NLEAGMVNRN LGRKT++AYLA+
Sbjct: 447 IFNECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAI 506

Query: 487 AEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPRE-GAEAEDDGHILAFVHDEKE 546
           AEPWPKVSGFAKVDLSTGE++ + YG ++YGGEP FLPR   ++ EDDG+I++FVHDE+ 
Sbjct: 507 AEPWPKVSGFAKVDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYIMSFVHDEES 566

Query: 547 WKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 588
           W+SEL IVNA+TL+LEA+VKLPSRVPYGFHGTF+ S  +  Q
Sbjct: 567 WESELHIVNAVTLELEATVKLPSRVPYGFHGTFVNSADMLNQ 588

[Close]

BLAST of MC10g0856 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 770.0 bits (1987), Expect = 1.4e-222
Identity = 372/558 (66.67%), Postives = 448/558 (80.29%), Query Frame = 0


Query: 33  VSCSLHTPSIVQIPKHSQTFRPPPLKTPVEKPVSPSTSAPEDWNFLQRAASMAIDAVENA 92
           + C++    + ++ K  Q F P    TP +         P   N  Q+AA++AIDA E A
Sbjct: 35  IKCTVQI-DVTELTKKRQLFTPRTTATPPQ-------HNPLRLNIFQKAAAIAIDAAERA 94

Query: 93  LLSAERKRPLPKTADPEVQIAGNFAPVPEQPVRKALPVTGRIPECIRGVYVRNGANPLHE 152
           L+S E+  PLPKTADP VQIAGN++PVPE  VR+ L V G IP+CI GVY+RNGANP+ E
Sbjct: 95  LISHEQDSPLPKTADPRVQIAGNYSPVPESSVRRNLTVEGTIPDCIDGVYIRNGANPMFE 154

Query: 153 PAAGHHLFDGDGMVHAVEFSDGGGVSYACRFTETQRLVQERAHGRPVFPKAIGELHGHSG 212
           P AGHHLFDGDGMVHAV+ ++ G  SYACRFT+T+RLVQE+  GRPVFPKAIGELHGHSG
Sbjct: 155 PTAGHHLFDGDGMVHAVKITN-GSASYACRFTKTERLVQEKRLGRPVFPKAIGELHGHSG 214

Query: 213 IARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIG 272
           IARL+LFYARGL GL+++ +G+GVANAGLVYFN RLLAMSEDDLPYQ+++T  GDL+T+G
Sbjct: 215 IARLMLFYARGLCGLINNQNGVGVANAGLVYFNNRLLAMSEDDLPYQLKITQTGDLQTVG 274

Query: 273 RFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQP 332
           R+DFDGQLKS MIAHPKLDPV+ E+ ALSYDV++KPYLKYF+FSP+G KSP++EIPL  P
Sbjct: 275 RYDFDGQLKSAMIAHPKLDPVTKELHALSYDVVKKPYLKYFRFSPDGVKSPELEIPLETP 334

Query: 333 TMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVIYDKNKISRFGILPKNATGAGDIKWI 392
           TM+HDFAITE FVVIPDQQVVFKL EMI G SPV++D  K+SR GI+PK+AT A  I W+
Sbjct: 335 TMIHDFAITENFVVIPDQQVVFKLGEMISGKSPVVFDGEKVSRLGIMPKDATEASQIIWV 394

Query: 393 EAPDCFCFHLWNAWEEPETNEVVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLTTGKST 452
            +P+ FCFHLWNAWE PET E+VVIGSCM+P DSIFNE +E+L+SVLSEIR+NL T K+T
Sbjct: 395 NSPETFCFHLWNAWESPETEEIVVIGSCMSPADSIFNERDESLRSVLSEIRINLRTRKTT 454

Query: 453 RRPIIPETEQVNLEAGMVNRNRLGRKTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYIYG 512
           RR ++   E VNLE GMVNRNRLGRKT+FA+LA+A PWPKVSGFAKVDL TGE++KYIYG
Sbjct: 455 RRSLL-VNEDVNLEIGMVNRNRLGRKTRFAFLAIAYPWPKVSGFAKVDLCTGEMKKYIYG 514

Query: 513 EQRYGGEPLFLP---REGAEAEDDGHILAFVHDEKEWKSELQIVNAITLKLEASVKLPSR 572
            ++YGGEP FLP     G E EDDG+I   VHDE+   SELQI+NA+ LKLEA++KLPSR
Sbjct: 515 GEKYGGEPFFLPGNSGNGEENEDDGYIFCHVHDEETKTSELQIINAVNLKLEATIKLPSR 574

Query: 573 VPYGFHGTFIGSKGLQKQ 588
           VPYGFHGTF+ S  L  Q
Sbjct: 575 VPYGFHGTFVDSNELVDQ 582

[Close]

BLAST of MC10g0856 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 647.9 bits (1670), Expect = 7.8e-186
Identity = 312/538 (57.99%), Postives = 404/538 (75.09%), Query Frame = 0


Query: 55  PPLKTPVEKPVSPSTSAPEDWNFLQRAASMAIDAVENAL-LSAERKRPLPKTADPEVQIA 114
           P L +PV  PV    + P + N LQ+ A+  +D +E+++ +  E+ RPLPK  DP VQ++
Sbjct: 44  PDLTSPVPSPVKLKPTYP-NLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQLS 103

Query: 115 GNFAPVPEQPVRKALPVTGRIPECIRGVYVRNGANPLHEPAAGHHLFDGDGMVHAVEFSD 174
           GNFAPV E PV+  L V G+IP C++GVY+RNGANP+  P AGHHLFDGDGM+HAV    
Sbjct: 104 GNFAPVNECPVQNGLEVVGQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIGF 163

Query: 175 GGGVSYACRFTETQRLVQERAHGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHG 234
              VSY+CR+T+T RLVQE A GR VFPK IGELHGHSG+ARL LF AR   GLVD + G
Sbjct: 164 DNQVSYSCRYTKTNRLVQETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRG 223

Query: 235 IGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTIGRFDFDGQLKSTMIAHPKLDPV 294
           +GVANAG+V+FNGRLLAMSEDDLPYQ+++   GDL+TIGRF FD Q+ S++IAHPK+D  
Sbjct: 224 MGVANAGVVFFNGRLLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDAT 283

Query: 295 SGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVV 354
           +G++  LSY+V++KP+L+Y KF+  G+K+ DVEI LP+PTM+HDFAITE FVVIPDQQ+V
Sbjct: 284 TGDLHTLSYNVLKKPHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQMV 343

Query: 355 FKLPEMIRGGSPVIYDKNKISRFGILPKNATGAGDIKWIEAPDCFCFHLWNAWEEPETNE 414
           FKL EMIRGGSPVIY K K++RFG+L K      DI W++ PDCFCFHLWNAWEE  T E
Sbjct: 344 FKLSEMIRGGSPVIYVKEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEE-RTEE 403

Query: 415 ----VVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLTTGKSTRRPIIPETEQVNLEAGM 474
               +VVIGSCM+PPD+IF+E  E  +  LSEIRLN+ T +S R+ I+     VNLEAG 
Sbjct: 404 GDPVIVVIGSCMSPPDTIFSESGEPTRVELSEIRLNMRTKESNRKVIV---TGVNLEAGH 463

Query: 475 VNRNRLGRKTQFAYLALAEPWPKVSGFAKVDLSTGEIRKYIYGEQRYGGEPLFLPREGAE 534
           +NR+ +GRK+QF Y+A+A+PWPK SG AKVD+  G + ++ YG  R+GGEP F+P EG  
Sbjct: 464 INRSYVGRKSQFVYIAIADPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVP-EGEG 523

Query: 535 AEDDGHILAFVHDEKEWKSELQIVNAITLKLEASVKLPSRVPYGFHGTFIGSKGLQKQ 588
            ED G+++ FV DE++ +SE  +V+A  +K  A+V+LP RVPYGFHGTF+    L++Q
Sbjct: 524 EEDKGYVMGFVRDEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKEQ 575

The following BLAST results are available for this feature:

BLAST of MC10g0856 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24023	5.9e-255	73.05	9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum O...	[more]
Q9M6E8	7.5e-250	71.52	9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=...	[more]
K4CJJ1	1.3e-249	72.79	9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum O...	[more]
Q9LRR7	6.4e-249	74.78	9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O...	[more]
Q9M9F5	1.6e-239	71.11	9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana O...	[more]

BLAST of MC10g0856 vs. NCBI nr


Match Name	Identity	Description
AFU91491.1	99.15	9-cis-epoxycarotenoid dioxygenase [Momordica charantia]	[more]
APO14273.1	88.76	9-cis-epoxycarotenoid dioxygenase NCED1 [Luffa aegyptiaca]	[more]
XP_038878047.1	87.78	9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic [Benincasa hispida]	[more]
XP_008465499.1	87.10	PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis ...	[more]
XP_023529573.1	87.44	9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucurbita pepo subs...	[more]

BLAST of MC10g0856 vs. ExPASy TrEMBL


Match Name	Identity	Description
K4KBB8	99.15	9-cis-epoxycarotenoid dioxygenase OS=Momordica charantia OX=3673 GN=NCED PE=2 SV...	[more]
A0A1L5JHV0	88.76	9-cis-epoxycarotenoid dioxygenase NCED1 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
A0A1S3CQH4	87.10	9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucumis melo OX=3...	[more]
A0A6J1ETU1	85.37	9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschat...	[more]
A0A6J1FPE2	87.37	9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschat...	[more]

BLAST of MC10g0856 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14440.1	4.5e-250	74.78	nine-cis-epoxycarotenoid dioxygenase 3	[more]
AT1G78390.1	1.1e-240	71.11	nine-cis-epoxycarotenoid dioxygenase 9	[more]
AT1G30100.1	1.2e-234	67.70	nine-cis-epoxycarotenoid dioxygenase 5	[more]
AT4G18350.1	1.4e-222	66.67	nine-cis-epoxycarotenoid dioxygenase 2	[more]
AT3G24220.1	7.8e-186	57.99	nine-cis-epoxycarotenoid dioxygenase 6	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004294	Carotenoid oxygenase	PFAM	PF03055	RPE65	coord: 117..579 e-value: 8.8E-117 score: 391.1
IPR004294	Carotenoid oxygenase	PANTHER	PTHR10543	BETA-CAROTENE DIOXYGENASE	coord: 52..587
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 47..72
None	No IPR available	PANTHER	PTHR10543:SF123	9-CIS-EPOXYCAROTENOID DIOXYGENASE NCED5, CHLOROPLASTIC-RELATED	coord: 52..587

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g0856.1	MC10g0856.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016121	carotene catabolic process
biological_process	GO:0010162	seed dormancy process
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0045549	9-cis-epoxycarotenoid dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen