MC10g0801 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g0801
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	nine-cis-epoxycarotenoid dioxygenase 4
Location	MC10: 6748030 .. 6749817 (-)
RNA-Seq Expression	MC10g0801
Synteny	MC10g0801

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACGCCATCTCATCCCCTTTCCTCTCCGGCGGAAACCTGCTCCTCTCTCCGGCAATCTCCATCTCCAGACCTTCCATTGCCACCGTCATATCCTCCGTTCTCACAGAAGAAACCCCCAAAACTGTCAAGAAAACAGGACCCCGGCCCTCCGATGCCGACTCCTCACCACTGAGAGCTACTCCTCCGCCGCCTGCTAAAGTTCCGGCAATGGCGAAGAGCTCTTCCACCAGACGAGTTGAGCCGTCGCTGCCGGCGAGGTTGTTCAATGCTTTCGACGACTTGATCAACAACTTCATAAATCCGCCGGTGAATCCGTCAGTGGACCCGCGGTATGTGCTCGCCGATAACTTCGCGCCGGTGGACGAGCTTCCGCCGACGGAGTGCGAGGTGATTCAGGGATCGTTGCCTCCATCTCTGAACGGCGCTTATATTCGTAATGGACCGAATCCGCAGTACCTTCCCCGTGGGCCCTACCACTTGTTCGACGGCGACGGAATGCTTCACTCTCTCCGGATCTCCGACGGCCGCGCTGTCCTCTGCAGCCGTTACGTCAAGACTTACAAATACACTCTGGAGCGTGACGTTGGGCATCCGGTTATCCCCAATGTATTCTCTGGCTTCAATGGCCTCACCGCCTCCGCCGCACGCAGCGCCGTCACCGCCGGCCGGATGTTAACCGGCCAGTTTGATCCGGCGAATGGGATTGGTCTTGCAAACACTAGTCTGGCTTACTTCGGCGACCGTCTTTACGCCCTTGGCGAGTCCGATTTGCCGTATCCGATTCGCTTGACTCCGACCGGCGACATAGAGACTCTCGACCGCCATGATTTCGACGGAAAACTCTCTATCAGTATGACTGCTCATCCGAAAGTTGATCCAGTCACCGGCGAGGCTTTCGCTTTCCGATACGGTCCTCTGCCTCCATTCTTAACCTTCTTCCGATTCGACAGAAGCGGTGCCAAACAATCCGATGTGCCGATTTTTTCAATGAGTCGCCCTTCCTTCCTCCACGATTTTGCAATCACCGAAAAGTACGCTGTATTTGGAGAAATTCAAATCGGATTCAATCCTATGCAGATGATTACAGAAGGACGGTCTCCAGTCGGATCCGATCCGTCGAAAGTTTGCAGAGTAGGAATAATTCCTCGATACGCAACCGACGAATCGAAAATGAAGTGGTTCGATGTGCCAGGATTCAACCTGATTCACGCCATTAACGCTTGGGATGAAGACGACGCCGTGGTAATGGTGGCGCCGAACATTCTGTCCGTCGAACACACGATGGAGAGAATGGATCTGGTTCACGCCTTAGTGGAGAAAGTCAGAATCGACCTGAAAACAGGGATAGTCACGCGGCGGCCATTATCGACCAGAAACCTGGACTTCGGAGTGATAAATCCGTCGTACGTCGGGAGGAAGAACAGGTTCGTATACGCCGGCGTCGGCGATCCGATGCCGAAGATTTCTGGAGTGGTGAAGCTTGACGTGTCTCAAGAAGAATGCCGAGACTGCATCGTTGCGAGCAGGATCTTCGGACCTGGTTGCTACGGCGGCGAGCCGTTCTTCGTGCCGAGGGAAGGGGAAAATGCCGGCGAAACGGCGGCGGAGGAGGACGACGGATACGTGGTCTCGTACGTTCACAACGAGAACACCGGCGAGTCGAGGTTCATCGTCATGGACGCGAAGTCGCCGGATCTCGCCATTGTCGCGGCGGTGAAGCTTCCGCGGCGGGTCCCTTACGGCTTCCACGGACTCTTTGTGAAAGAAAGTGACCTCAATAAGCTA

mRNA sequence

ATGGACGCCATCTCATCCCCTTTCCTCTCCGGCGGAAACCTGCTCCTCTCTCCGGCAATCTCCATCTCCAGACCTTCCATTGCCACCGTCATATCCTCCGTTCTCACAGAAGAAACCCCCAAAACTGTCAAGAAAACAGGACCCCGGCCCTCCGATGCCGACTCCTCACCACTGAGAGCTACTCCTCCGCCGCCTGCTAAAGTTCCGGCAATGGCGAAGAGCTCTTCCACCAGACGAGTTGAGCCGTCGCTGCCGGCGAGGTTGTTCAATGCTTTCGACGACTTGATCAACAACTTCATAAATCCGCCGGTGAATCCGTCAGTGGACCCGCGGTATGTGCTCGCCGATAACTTCGCGCCGGTGGACGAGCTTCCGCCGACGGAGTGCGAGGTGATTCAGGGATCGTTGCCTCCATCTCTGAACGGCGCTTATATTCGTAATGGACCGAATCCGCAGTACCTTCCCCGTGGGCCCTACCACTTGTTCGACGGCGACGGAATGCTTCACTCTCTCCGGATCTCCGACGGCCGCGCTGTCCTCTGCAGCCGTTACGTCAAGACTTACAAATACACTCTGGAGCGTGACGTTGGGCATCCGGTTATCCCCAATGTATTCTCTGGCTTCAATGGCCTCACCGCCTCCGCCGCACGCAGCGCCGTCACCGCCGGCCGGATGTTAACCGGCCAGTTTGATCCGGCGAATGGGATTGGTCTTGCAAACACTAGTCTGGCTTACTTCGGCGACCGTCTTTACGCCCTTGGCGAGTCCGATTTGCCGTATCCGATTCGCTTGACTCCGACCGGCGACATAGAGACTCTCGACCGCCATGATTTCGACGGAAAACTCTCTATCAGTATGACTGCTCATCCGAAAGTTGATCCAGTCACCGGCGAGGCTTTCGCTTTCCGATACGGTCCTCTGCCTCCATTCTTAACCTTCTTCCGATTCGACAGAAGCGGTGCCAAACAATCCGATGTGCCGATTTTTTCAATGAGTCGCCCTTCCTTCCTCCACGATTTTGCAATCACCGAAAAGTACGCTGTATTTGGAGAAATTCAAATCGGATTCAATCCTATGCAGATGATTACAGAAGGACGGTCTCCAGTCGGATCCGATCCGTCGAAAGTTTGCAGAGTAGGAATAATTCCTCGATACGCAACCGACGAATCGAAAATGAAGTGGTTCGATGTGCCAGGATTCAACCTGATTCACGCCATTAACGCTTGGGATGAAGACGACGCCGTGGTAATGGTGGCGCCGAACATTCTGTCCGTCGAACACACGATGGAGAGAATGGATCTGGTTCACGCCTTAGTGGAGAAAGTCAGAATCGACCTGAAAACAGGGATAGTCACGCGGCGGCCATTATCGACCAGAAACCTGGACTTCGGAGTGATAAATCCGTCGTACGTCGGGAGGAAGAACAGGTTCGTATACGCCGGCGTCGGCGATCCGATGCCGAAGATTTCTGGAGTGGTGAAGCTTGACGTGTCTCAAGAAGAATGCCGAGACTGCATCGTTGCGAGCAGGATCTTCGGACCTGGTTGCTACGGCGGCGAGCCGTTCTTCGTGCCGAGGGAAGGGGAAAATGCCGGCGAAACGGCGGCGGAGGAGGACGACGGATACGTGGTCTCGTACGTTCACAACGAGAACACCGGCGAGTCGAGGTTCATCGTCATGGACGCGAAGTCGCCGGATCTCGCCATTGTCGCGGCGGTGAAGCTTCCGCGGCGGGTCCCTTACGGCTTCCACGGACTCTTTGTGAAAGAAAGTGACCTCAATAAGCTA

Coding sequence (CDS)

ATGGACGCCATCTCATCCCCTTTCCTCTCCGGCGGAAACCTGCTCCTCTCTCCGGCAATCTCCATCTCCAGACCTTCCATTGCCACCGTCATATCCTCCGTTCTCACAGAAGAAACCCCCAAAACTGTCAAGAAAACAGGACCCCGGCCCTCCGATGCCGACTCCTCACCACTGAGAGCTACTCCTCCGCCGCCTGCTAAAGTTCCGGCAATGGCGAAGAGCTCTTCCACCAGACGAGTTGAGCCGTCGCTGCCGGCGAGGTTGTTCAATGCTTTCGACGACTTGATCAACAACTTCATAAATCCGCCGGTGAATCCGTCAGTGGACCCGCGGTATGTGCTCGCCGATAACTTCGCGCCGGTGGACGAGCTTCCGCCGACGGAGTGCGAGGTGATTCAGGGATCGTTGCCTCCATCTCTGAACGGCGCTTATATTCGTAATGGACCGAATCCGCAGTACCTTCCCCGTGGGCCCTACCACTTGTTCGACGGCGACGGAATGCTTCACTCTCTCCGGATCTCCGACGGCCGCGCTGTCCTCTGCAGCCGTTACGTCAAGACTTACAAATACACTCTGGAGCGTGACGTTGGGCATCCGGTTATCCCCAATGTATTCTCTGGCTTCAATGGCCTCACCGCCTCCGCCGCACGCAGCGCCGTCACCGCCGGCCGGATGTTAACCGGCCAGTTTGATCCGGCGAATGGGATTGGTCTTGCAAACACTAGTCTGGCTTACTTCGGCGACCGTCTTTACGCCCTTGGCGAGTCCGATTTGCCGTATCCGATTCGCTTGACTCCGACCGGCGACATAGAGACTCTCGACCGCCATGATTTCGACGGAAAACTCTCTATCAGTATGACTGCTCATCCGAAAGTTGATCCAGTCACCGGCGAGGCTTTCGCTTTCCGATACGGTCCTCTGCCTCCATTCTTAACCTTCTTCCGATTCGACAGAAGCGGTGCCAAACAATCCGATGTGCCGATTTTTTCAATGAGTCGCCCTTCCTTCCTCCACGATTTTGCAATCACCGAAAAGTACGCTGTATTTGGAGAAATTCAAATCGGATTCAATCCTATGCAGATGATTACAGAAGGACGGTCTCCAGTCGGATCCGATCCGTCGAAAGTTTGCAGAGTAGGAATAATTCCTCGATACGCAACCGACGAATCGAAAATGAAGTGGTTCGATGTGCCAGGATTCAACCTGATTCACGCCATTAACGCTTGGGATGAAGACGACGCCGTGGTAATGGTGGCGCCGAACATTCTGTCCGTCGAACACACGATGGAGAGAATGGATCTGGTTCACGCCTTAGTGGAGAAAGTCAGAATCGACCTGAAAACAGGGATAGTCACGCGGCGGCCATTATCGACCAGAAACCTGGACTTCGGAGTGATAAATCCGTCGTACGTCGGGAGGAAGAACAGGTTCGTATACGCCGGCGTCGGCGATCCGATGCCGAAGATTTCTGGAGTGGTGAAGCTTGACGTGTCTCAAGAAGAATGCCGAGACTGCATCGTTGCGAGCAGGATCTTCGGACCTGGTTGCTACGGCGGCGAGCCGTTCTTCGTGCCGAGGGAAGGGGAAAATGCCGGCGAAACGGCGGCGGAGGAGGACGACGGATACGTGGTCTCGTACGTTCACAACGAGAACACCGGCGAGTCGAGGTTCATCGTCATGGACGCGAAGTCGCCGGATCTCGCCATTGTCGCGGCGGTGAAGCTTCCGCGGCGGGTCCCTTACGGCTTCCACGGACTCTTTGTGAAAGAAAGTGACCTCAATAAGCTA

Protein sequence

MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDADSSPLRATPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLADNFAPVDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGIGLANTSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVTGEAFAFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGFNPMQMITEGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWDEDDAVVMVAPNILSVEHTMERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVINPSYVGRKNRFVYAGVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREGENAGETAAEEDDGYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESDLNKL

Homology

[Close]

BLAST of MC10g0801 vs. ExPASy Swiss-Prot
Match: O49675 (Probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CCD4 PE=1 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 5.7e-229
Identity = 389/604 (64.40%), Postives = 480/604 (79.47%), Query Frame = 0


Query: 1   MDAISSPFLSGGNLLLSPAI---SISRPSIATVISSVLTEETPKTVKKTGPRPSDADSSP 60
           MD++SS         L  ++     S P++  + S+V+ E +P T       PSD +   
Sbjct: 1   MDSVSSSSFLSSTFSLHHSLLRRRSSSPTLLRINSAVVEERSPIT------NPSDNNDRR 60

Query: 61  LRATPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLADN 120
            +           +  S    R E +L   LF   +D+IN FI+PP  PSVDP++VL+DN
Sbjct: 61  NKPKTLHNRTNHTLVSSPPKLRPEMTLATALFTTVEDVINTFIDPPSRPSVDPKHVLSDN 120

Query: 121 FAPV-DELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDG 180
           FAPV DELPPT+CE+I G+LP SLNGAYIRNGPNPQ+LPRGPYHLFDGDGMLH+++I +G
Sbjct: 121 FAPVLDELPPTDCEIIHGTLPLSLNGAYIRNGPNPQFLPRGPYHLFDGDGMLHAIKIHNG 180

Query: 181 RAVLCSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGI 240
           +A LCSRYVKTYKY +E+  G PV+PNVFSGFNG+TAS AR A+TA R+LTGQ++P NGI
Sbjct: 181 KATLCSRYVKTYKYNVEKQTGAPVMPNVFSGFNGVTASVARGALTAARVLTGQYNPVNGI 240

Query: 241 GLANTSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVT 300
           GLANTSLA+F +RL+ALGESDLPY +RLT +GDIET+ R+DFDGKL++SMTAHPK DP+T
Sbjct: 241 GLANTSLAFFSNRLFALGESDLPYAVRLTESGDIETIGRYDFDGKLAMSMTAHPKTDPIT 300

Query: 301 GEAFAFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIG- 360
           GE FAFRYGP+PPFLT+FRFD +G KQ DVPIFSM+ PSFLHDFAIT+++A+F EIQ+G 
Sbjct: 301 GETFAFRYGPVPPFLTYFRFDSAGKKQRDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGM 360

Query: 361 -FNPMQMITEGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWDEDD- 420
             N + ++ EG SPVG+D  K  R+G+IP+YA DES+MKWF+VPGFN+IHAINAWDEDD 
Sbjct: 361 RMNMLDLVLEGGSPVGTDNGKTPRLGVIPKYAGDESEMKWFEVPGFNIIHAINAWDEDDG 420

Query: 421 -AVVMVAPNILSVEHTMERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVINPSYVG 480
            +VV++APNI+S+EHT+ERMDLVHALVEKV+IDL TGIV R P+S RNLDF VINP+++G
Sbjct: 421 NSVVLIAPNIMSIEHTLERMDLVHALVEKVKIDLVTGIVRRHPISARNLDFAVINPAFLG 480

Query: 481 RKNRFVYAGVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREGENAG 540
           R +R+VYA +GDPMPKISGVVKLDVS+ +  DC VA R++G GCYGGEPFFV R+    G
Sbjct: 481 RCSRYVYAAIGDPMPKISGVVKLDVSKGDRDDCTVARRMYGSGCYGGEPFFVARD---PG 540

Query: 541 ETAAEEDDGYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESD 597
              AEEDDGYVV+YVH+E TGES+F+VMDAKSP+L IVAAV+LPRRVPYGFHGLFVKESD
Sbjct: 541 NPEAEEDDGYVVTYVHDEVTGESKFLVMDAKSPELEIVAAVRLPRRVPYGFHGLFVKESD 595

[Close]

BLAST of MC10g0801 vs. ExPASy Swiss-Prot
Match: Q6YVJ0 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 4.4e-197
Identity = 351/592 (59.29%), Postives = 439/592 (74.16%), Query Frame = 0


Query: 14  LLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDADSSPLRATPPP--PAKVPAM 73
           +L SP ++ +R    T       +E  + V KT    +   ++  RA P    P + PA 
Sbjct: 57  VLTSPLVTTTRTPKQT------EQEDEQLVAKTKTTRTVIATTNGRAAPSQSRPRRRPAP 116

Query: 74  AKSSSTRRVEPSLPARLFNAFDDLINNFINPP-VNPSVDPRYVLADNFAPVDELPPTECE 133
           A ++S      SLP    NA +++IN FI+PP + P+VDPR VL  NF PVDELPPT C 
Sbjct: 117 AAAASA----ASLPMTFCNALEEVINTFIDPPALRPAVDPRNVLTSNFVPVDELPPTPCP 176

Query: 134 VIQGSLPPSL-NGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRI-----SDGRAVLCSRY 193
           V++G++P  L  GAYIRNGPNPQ+LPRGP+HLFDGDGMLHSL +     S    VLCSRY
Sbjct: 177 VVRGAIPRCLAGGAYIRNGPNPQHLPRGPHHLFDGDGMLHSLLLPSPASSGDDPVLCSRY 236

Query: 194 VKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGIGLANTSLA 253
           V+TYKY +ERD G PV+PNVFSGF+G+ A  AR AV A R+LTGQ +P  G+GLANTSLA
Sbjct: 237 VQTYKYLVERDAGAPVLPNVFSGFHGV-AGMARGAVVAARVLTGQMNPLEGVGLANTSLA 296

Query: 254 YFGDRLYALGESDLPYPIRLTP-TGDIETLDRHDFDGKLSISMTAHPKVDPVTGEAFAFR 313
           YF  RLYALGESDLPY +R+ P TG++ T  R DF G+L + MTAHPK DPVTGE FAFR
Sbjct: 297 YFAGRLYALGESDLPYAVRVHPDTGEVTTHGRCDFGGRLVMGMTAHPKKDPVTGELFAFR 356

Query: 314 YGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGFNPMQMIT 373
           YGP+PPF+T+FRFD +G K +DVPIFS+ +PSFLHDFAITE+YA+F EIQI   PM M+ 
Sbjct: 357 YGPVPPFVTYFRFDPAGNKGADVPIFSVQQPSFLHDFAITERYAIFPEIQIVMKPMDMVV 416

Query: 374 EGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWDE--DDAVVMVAPN 433
            G SPVGSDP KV R+G+IPRYATDES+M+WF+VPGFN++H++NAW+E   + +V+VAPN
Sbjct: 417 GGGSPVGSDPGKVPRLGVIPRYATDESEMRWFEVPGFNIMHSVNAWEEAGGEELVLVAPN 476

Query: 434 ILSVEHTMERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVINPSYVGRKNRFVYAG 493
           +LS+EH +E M+LVH+ VEKVRI+L+TG+VTR PL+  N DF VINP+++GR+NR+ Y G
Sbjct: 477 VLSIEHALEHMELVHSCVEKVRINLRTGVVTRTPLAAGNFDFPVINPAFLGRRNRYGYFG 536

Query: 494 VGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREGENAGETAAEEDDG 553
           VGDP PKI GV KLD  +    DC VA R FGPGC+ GEPFFV  + E  G     EDDG
Sbjct: 537 VGDPAPKIGGVAKLDFDRAGEGDCTVAQRDFGPGCFAGEPFFVADDVEGNG----NEDDG 596

Query: 554 YVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESDL 594
           Y+V YVH+E TGE+RF+VMDA+SPDL IVA V+LP RVPYGFHGLFV +++L
Sbjct: 597 YLVCYVHDEATGENRFVVMDARSPDLEIVAEVQLPGRVPYGFHGLFVTQAEL 633

[Close]

BLAST of MC10g0801 vs. ExPASy Swiss-Prot
Match: Q5MBR6 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 8.9e-166
Identity = 296/523 (56.60%), Postives = 367/523 (70.17%), Query Frame = 0


Query: 83  SLPARLFNAFDDLINNFIN-PPVNPSVDPRYVLADNFAPVDELPPTECEVIQGSLPPSL- 142
           SLP  + N  D +IN FI+ P   P+VDPR VL+ NFAPVDELPPT C VI+GS+P  L 
Sbjct: 55  SLPMAICNTVDKVINRFIDLPEQRPTVDPRRVLSGNFAPVDELPPTSCHVIRGSIPSCLA 114

Query: 143 NGAYIRNGPNPQY-LPRGPYHLFDGDGMLHSLRISDGRA------VLCSRYVKTYKYTLE 202
            G YIRNGPNPQ+ LP+  +HLFDGDGMLHSL I    +      VLCSRYV TYKY LE
Sbjct: 115 GGVYIRNGPNPQHRLPQRTHHLFDGDGMLHSLLIPSASSTLLSEPVLCSRYVHTYKYLLE 174

Query: 203 RDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGIGLANTSLAYFGDRLYAL 262
           R+ G PV+PN F+GF+G+ A  AR+ V   R+L GQ +   G GLANTS+  F D LYAL
Sbjct: 175 RETGGPVLPNFFAGFHGV-AGLARAVVMIARVLAGQINLNKGFGLANTSITLFADCLYAL 234

Query: 263 GESDLPYPIRLTP-TGDIETLDRHDFDGKLSISMTAHPKVDPVTGEAFAFRYGPLPPFLT 322
            ESDLPY + + P  G++ TL R DF G LS  MTAHPK DPVT E FAFRY    PF+T
Sbjct: 235 CESDLPYSMHINPANGEVTTLGRCDFGGDLSFRMTAHPKKDPVTMELFAFRYNVFQPFIT 294

Query: 323 FFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGFNPMQMITEGRSPVGSD 382
           +F FDR+G+K +DVPI S+ +PS +HDFAITE+YA+F E Q+  NPM M+  G S VG D
Sbjct: 295 YFWFDRAGSKVADVPILSLQKPSVMHDFAITERYAIFPESQLIVNPMDMVMRGSSLVGLD 354

Query: 383 PSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWDEDDA--VVMVAPNILSVEHTME 442
            + V R+G++PRYA DES M+WF+VP FN++H  N W+E D   +V+VAPNILS+EH + 
Sbjct: 355 RTMVPRIGVLPRYAKDESDMRWFEVPRFNMLHTTNGWEEADGEEIVLVAPNILSIEHMLG 414

Query: 443 RMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVINPSYVGRKNRFVYAGVGDPMPKIS 502
            M+L+ A V+ VRI+L TG V+   LS  +L+FGVI+  YVGRKNR+ Y GV  P+PKI 
Sbjct: 415 NMELMRARVDMVRINLCTGDVSCTALSPESLEFGVIHQGYVGRKNRYGYFGVSGPLPKIK 474

Query: 503 GVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREGENAGETAAEEDDGYVVSYVHNE 562
           G+ KLD       DC V  R FG GC+ GEPFFVP   +  G     ED GYVV Y H E
Sbjct: 475 GIRKLDFDLVGSGDCTVGRRDFGLGCFAGEPFFVPDNIDGYG----NEDSGYVVCYTHEE 534

Query: 563 NTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESDL 594
           +TGES F+VMDAKSP+L IVA V+LP R+PYGFHG+FVK+++L
Sbjct: 535 DTGESWFVVMDAKSPELDIVAEVQLPSRIPYGFHGIFVKQAEL 572

[Close]

BLAST of MC10g0801 vs. ExPASy Swiss-Prot
Match: Q84K96 (Zeaxanthin 7,8(7',8')-cleavage dioxygenase, chromoplastic OS=Crocus sativus OX=82528 GN=ZCD PE=1 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 2.5e-120
Identity = 213/369 (57.72%), Postives = 270/369 (73.17%), Query Frame = 0


Query: 229 QFDPANGIGLANTSLAYFGDRLYALGESDLPYPIRLTP-TGDIETLDRHDFDGKLSISMT 288
           Q DP  GIGLANTSL +   RL+AL E DLPY +RL+P  GDI T+ R + +   + S T
Sbjct: 2   QVDPTKGIGLANTSLQFSNGRLHALCEYDLPYVVRLSPEDGDISTVGRIE-NNVSTKSTT 61

Query: 289 AHPKVDPVTGEAFAFRYGPLPPFLTFFRFDRSGAKQS-DVPIFSMSRPSFLHDFAITEKY 348
           AHPK DPVTGE F+F YGP+ P++T+ R+D  G K   DVPIFS   PSF+HDFAITE Y
Sbjct: 62  AHPKTDPVTGETFSFSYGPIQPYVTYSRYDCDGKKSGPDVPIFSFKEPSFVHDFAITEHY 121

Query: 349 AVFGEIQIGFNPMQMITEGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAI 408
           AVF +IQI   P + I  GR  +G D  KV R+G++PRYAT +S+M+WFDVPGFN++H +
Sbjct: 122 AVFPDIQIVMKPAE-IVRGRRMIGPDLEKVPRLGLLPRYATSDSEMRWFDVPGFNMVHVV 181

Query: 409 NAWDED--DAVVMVAPNILSVEHTMERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFG 468
           NAW+E+  + VV+VAPN+  +E+ ++R DL+H  VE  RI+LK+G V+R  LS  NLDFG
Sbjct: 182 NAWEEEGGEVVVIVAPNVSPIENAIDRFDLLHVSVEMARIELKSGSVSRTLLSAENLDFG 241

Query: 469 VINPSYVGRKNRFVYAGVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFV 528
           VI+  Y GRK+R+ Y GVGDPMPKI GVVK+D       +C+VA R FG GC+GGEPFFV
Sbjct: 242 VIHRGYSGRKSRYAYLGVGDPMPKIRGVVKVDFELAGRGECVVARREFGVGCFGGEPFFV 301

Query: 529 PREGENAGETAAEEDDGYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFH 588
           P   + +G    EEDDGYVVSY+H+E  GES F+VMDA+SP+L I+A V LPRRVPYGFH
Sbjct: 302 PASSKKSG---GEEDDGYVVSYLHDEGKGESSFVVMDARSPELEILAEVVLPRRVPYGFH 361

Query: 589 GLFVKESDL 594
           GLFV E++L
Sbjct: 362 GLFVTEAEL 365

[Close]

BLAST of MC10g0801 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 2.5e-107
Identity = 230/607 (37.89%), Postives = 339/607 (55.85%), Query Frame = 0


Query: 3   AISSPFLSGGNLLLSPAISISRPSIATVISS--VLTEETPKTVKKTGPRPSDADSSPLRA 62
           A+S  +L G +    P +S S+ S  +  SS  + +  T K    +      A   P ++
Sbjct: 9   AVSGRWLGGNH--TQPPLSSSQSSDLSYCSSLPMASRVTRKLNVSSALHTPPALHFPKQS 68

Query: 63  TPPPPAKVPAMAKSSSTRRV---EPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLADN 122
           +  P   V   AK S+T+++   + +  A L  A   L+++    P+  + DP   +A N
Sbjct: 69  SNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGN 128

Query: 123 FAPVDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGR 182
           FAPV+E P      + G LP S+ G Y+RNG NP + P   +H FDGDGM+H+++   G 
Sbjct: 129 FAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKFEHGS 188

Query: 183 AVLCSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGIG 242
           A    R+ +T ++  ER +G PV P      +G T   AR  +   R   G  DPA+G G
Sbjct: 189 ASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHT-GIARLMLFYARAAAGIVDPAHGTG 248

Query: 243 LANTSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVTG 302
           +AN  L YF  RL A+ E DLPY +++TP GD++T+ R DFDG+L  +M AHPKVDP +G
Sbjct: 249 VANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESG 308

Query: 303 EAFAFRYGPL-PPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGF 362
           E FA  Y  +  P+L +FRF   G K  DV I  + +P+ +HDFAITE + V  + Q+ F
Sbjct: 309 ELFALSYDVVSKPYLKYFRFSPDGTKSPDVEI-QLDQPTMMHDFAITENFVVVPDQQVVF 368

Query: 363 NPMQMITEGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWD--EDDA 422
              +MI  G SPV  D +KV R GI+ +YA D S +KW D P     H  NAW+  E D 
Sbjct: 369 KLPEMI-RGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDE 428

Query: 423 VVMVAPNILSVEHTMERMD-LVHALVEKVRIDLKTGIVTRRPLSTR-----NLDFGVINP 482
           VV++   +   +      D  + +++ ++R++LKTG  TRRP+ +      NL+ G++N 
Sbjct: 429 VVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNR 488

Query: 483 SYVGRKNRFVYAGVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREG 542
           + +GRK +F Y  + +P PK+SG  K+D++  E     V   ++G   YGGEP F+P EG
Sbjct: 489 NMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGE-----VKKHLYGDNRYGGEPLFLPGEG 548

Query: 543 ENAGETAAEEDDGYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFV 596
                   EED+GY++ +VH+E T +S   +++A S  L + A VKLP RVPYGFHG F+
Sbjct: 549 -------GEEDEGYILCFVHDEKTWKSELQIVNAVS--LEVEATVKLPSRVPYGFHGTFI 596

[Close]

BLAST of MC10g0801 vs. NCBI nr
Match: AFU91490.1 (carotenoid cleavage dioxygenase 4 [Momordica charantia])

HSP 1 Score: 1170 bits (3028), Expect = 0.0
Identity = 586/596 (98.32%), Postives = 588/596 (98.66%), Query Frame = 0


Query: 1   MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDADSSPLRA 60
           MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDADSSPLRA
Sbjct: 1   MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDADSSPLRA 60

Query: 61  TPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLADNFAP 120
           TPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLADNFAP
Sbjct: 61  TPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLADNFAP 120

Query: 121 VDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVL 180
           VDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVL
Sbjct: 121 VDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVL 180

Query: 181 CSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGIGLAN 240
           CSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGIGLAN
Sbjct: 181 CSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGIGLAN 240

Query: 241 TSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVTGEAF 300
           TSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVTGEAF
Sbjct: 241 TSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVTGEAF 300

Query: 301 AFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGFNPMQ 360
           AFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGE QIGFNPMQ
Sbjct: 301 AFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGETQIGFNPMQ 360

Query: 361 MITEGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWDEDDAVVMVAP 420
           MITEGRSPVGS+PSKVCR GIIPRYATDESKMKWFDVPGFNLIHAINAWDE DAVVMVAP
Sbjct: 361 MITEGRSPVGSNPSKVCRAGIIPRYATDESKMKWFDVPGFNLIHAINAWDEYDAVVMVAP 420

Query: 421 NILSVEHTMERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVINPSYVGRKNRFVYA 480
           NILSVEHTMERMDLVHALVEK RIDLKTGIVTRR LSTRNLDFGVINPSYVGRKNRFVYA
Sbjct: 421 NILSVEHTMERMDLVHALVEKARIDLKTGIVTRRSLSTRNLDFGVINPSYVGRKNRFVYA 480

Query: 481 GVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREGENAGETAAEEDD 540
           GVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPF VPREGENAGETAAEE D
Sbjct: 481 GVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFLVPREGENAGETAAEEGD 540

Query: 541 GYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESDLNKL 596
           GYVVSYVHNENTGESRFIVMDAKSP+LAIVAAVKLPRRVPYGF GLFVKESDLNKL
Sbjct: 541 GYVVSYVHNENTGESRFIVMDAKSPNLAIVAAVKLPRRVPYGFLGLFVKESDLNKL 596

[Close]

BLAST of MC10g0801 vs. NCBI nr
Match: XP_038897022.1 (probable carotenoid cleavage dioxygenase 4, chloroplastic [Benincasa hispida])

HSP 1 Score: 1041 bits (2693), Expect = 0.0
Identity = 525/597 (87.94%), Postives = 554/597 (92.80%), Query Frame = 0


Query: 1   MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDADSS---- 60
           MDAISS FLSGGNLLLSP ISISRP I+T I SVLTEET   VKKT PRP DADS     
Sbjct: 1   MDAISSSFLSGGNLLLSPPISISRPPISTAIYSVLTEET---VKKTAPRP-DADSPSPSL 60

Query: 61  PLRATPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLAD 120
           P RA+PPP     A+AK SSTRR+EPSLPAR FNAFDDLINNFINPPV+PSVDPRYVLAD
Sbjct: 61  PRRASPPP-----AIAKGSSTRRIEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLAD 120

Query: 121 NFAPVDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDG 180
           NFAPVDELPPTECEVI GSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+G
Sbjct: 121 NFAPVDELPPTECEVIHGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNG 180

Query: 181 RAVLCSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGI 240
           RAVLCSRYVKTYKYTLERD GHPV PNVFSGFNGLTASAAR AV AGR+LTGQF+PANGI
Sbjct: 181 RAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQFNPANGI 240

Query: 241 GLANTSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVT 300
           GLANTSLA+FGDRLYALGESDLPYPIRLTP GDIETL RHDFDGKL++SMTAHPKVD  T
Sbjct: 241 GLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLTMSMTAHPKVDSDT 300

Query: 301 GEAFAFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGF 360
           GEAFAFRYGPLPPFLTFFRFD++GAKQSDVPIFSM+RPSFLHDFAIT+KYAVF EIQIGF
Sbjct: 301 GEAFAFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGF 360

Query: 361 NPMQMITEGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWDEDDAVV 420
           NPMQMI EGRSPVGSDPSK+ RVG+IPRYA DESKMKWFDVPG NL+HAINAWDEDDAVV
Sbjct: 361 NPMQMI-EGRSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLVHAINAWDEDDAVV 420

Query: 421 MVAPNILSVEHTMERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVINPSYVGRKNR 480
           ++APNILSVEHT+ERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVI+PSYVG+KNR
Sbjct: 421 LIAPNILSVEHTLERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVIHPSYVGKKNR 480

Query: 481 FVYAGVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREGENAGETAA 540
           FVYAGVGDPMPKISGVVKLDVSQEE RDCIVA RIFGPGCYGGEPFFVPRE E++ ET A
Sbjct: 481 FVYAGVGDPMPKISGVVKLDVSQEERRDCIVACRIFGPGCYGGEPFFVPRERESSEETPA 540

Query: 541 EEDDGYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESDL 593
           EEDDGYVVSYVH+EN+GESRFIVMDAKSP+L I+AAVKLPRRVPYGFHGLFVKESDL
Sbjct: 541 EEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDL 587

[Close]

BLAST of MC10g0801 vs. NCBI nr
Match: XP_022931473.1 (probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita moschata] >AXM43901.1 carotenoid cleavage dioxygenase [Cucurbita moschata])

HSP 1 Score: 1036 bits (2678), Expect = 0.0
Identity = 512/600 (85.33%), Postives = 551/600 (91.83%), Query Frame = 0


Query: 1   MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDAD----SS 60
           MDAISSPFLSGGNLLLSP+IS SR +++T I SVL EETP+TVKK   RP DAD    S 
Sbjct: 1   MDAISSPFLSGGNLLLSPSISKSRTAVSTAICSVLAEETPRTVKKPASRP-DADFPSPSL 60

Query: 61  PLRATPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLAD 120
             RATPPP     A+AK SSTRRVEPS+PA +FNAFDDLINNFINPP NPS DPRYVLAD
Sbjct: 61  SQRATPPP-----AVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLAD 120

Query: 121 NFAPVDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDG 180
           NFAPVDELPPT+CEVIQGSLP SLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+G
Sbjct: 121 NFAPVDELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNG 180

Query: 181 RAVLCSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGI 240
           RAVLCSRYVKTYKYTLERD GHPVIPNVFSGFNGL ASAAR AVTAGRM TGQF+P NGI
Sbjct: 181 RAVLCSRYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGI 240

Query: 241 GLANTSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVT 300
           GLANTSLA+FGDRLYALGESDLPYPIRLTP GDIETL RHDFDGKLS+SMTAHPK+D  T
Sbjct: 241 GLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDT 300

Query: 301 GEAFAFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGF 360
           GEAFAFRYGPLPPFLTFFRFD++GAKQ DVPIFSM+RPSFLHDFAIT+KYAVF EIQIGF
Sbjct: 301 GEAFAFRYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGF 360

Query: 361 NPMQMITEGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWDEDDAVV 420
           NPMQMI EGRSPVG+DPSK+ R+G+IPRYA DESKMKWFDVPG NL+HAINAWDEDDAV+
Sbjct: 361 NPMQMIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVI 420

Query: 421 MVAPNILSVEHTMERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVINPSYVGRKNR 480
           +VAPN+LSVEHTMERMDLVHALVEK+RIDLKTGI+TRRPLSTRNLDFGVINP YVG+KNR
Sbjct: 421 LVAPNVLSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLYVGKKNR 480

Query: 481 FVYAGVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREGENAGETAA 540
           FVYAGVGDPMPKISGVVKLDVSQEE RDCIVA+R+FGP CYGGEPFFVPRE EN+ E AA
Sbjct: 481 FVYAGVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDEMAA 540

Query: 541 EEDDGYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESDLNKL 596
           EEDDGYVVSYVH+EN+G+SRF+VMDAKSP+L I+A VKLPRRVPYGFHGLFVKESDL+KL
Sbjct: 541 EEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

[Close]

BLAST of MC10g0801 vs. NCBI nr
Match: AXM43896.1 (carotenoid cleavage dioxygenase [Cucurbita pepo])

HSP 1 Score: 1035 bits (2676), Expect = 0.0
Identity = 513/600 (85.50%), Postives = 553/600 (92.17%), Query Frame = 0


Query: 1   MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDAD----SS 60
           MDAISSPFLSGGNLLLS  IS SR +++T I SVL EETP+TVKK   RP DAD    S 
Sbjct: 1   MDAISSPFLSGGNLLLSTPISKSRTAVSTAICSVLAEETPRTVKKPASRP-DADFPSPSL 60

Query: 61  PLRATPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLAD 120
             RATPPP     A+AK SSTRRVEPS+PA +FNAFDDLINNFINPPV+PSVDPRYVLAD
Sbjct: 61  SQRATPPP-----AVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPVSPSVDPRYVLAD 120

Query: 121 NFAPVDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDG 180
           NFAPVDELPPTECEVIQGSLP SLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+G
Sbjct: 121 NFAPVDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNG 180

Query: 181 RAVLCSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGI 240
           RAVLCSRYVKTYKYTLERD GHPV PNVFSGFNGLTASAAR AV AGR+LTGQ++PANGI
Sbjct: 181 RAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGI 240

Query: 241 GLANTSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVT 300
           GLANTS+A+FGDRLYALGESDLPYPIRLTP GDIETL RHDFDGKLS+SMTAHPK+D  T
Sbjct: 241 GLANTSVAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDT 300

Query: 301 GEAFAFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGF 360
           GEAFAFRYGPLPPFLTFFRFD++GAKQ DVPIFSM+RPSFLHDFAIT+KYAVF EIQIGF
Sbjct: 301 GEAFAFRYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGF 360

Query: 361 NPMQMITEGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWDEDDAVV 420
           NPMQMI EGRSPVG+DPSK+ R+G+IPRYA DESKMKWFDVPG NL+HAINAWDEDDAV+
Sbjct: 361 NPMQMIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVI 420

Query: 421 MVAPNILSVEHTMERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVINPSYVGRKNR 480
           +VAPNILSVEHTMERMDLVHALVEK+RIDLKTGI+TRRPLSTRNLDFGVINP YVG+KNR
Sbjct: 421 LVAPNILSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLYVGKKNR 480

Query: 481 FVYAGVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREGENAGETAA 540
           FVYAGVGDPMPKISGVVKLDVSQEE RDCIVA+R+FGP CYGGEPFFVPRE EN+ ETAA
Sbjct: 481 FVYAGVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDETAA 540

Query: 541 EEDDGYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESDLNKL 596
           EEDDGYVVSYVH+EN+G+SRF+VMDAKSP+L I+A VKLPRRVPYGFHGLFVKESDL+KL
Sbjct: 541 EEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

[Close]

BLAST of MC10g0801 vs. NCBI nr
Match: KAG7015104.1 (putative carotenoid cleavage dioxygenase 4, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1030 bits (2664), Expect = 0.0
Identity = 510/600 (85.00%), Postives = 550/600 (91.67%), Query Frame = 0


Query: 1   MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDAD----SS 60
           MDA+SSPFLSGGNLLLSP IS SR +++T I SVL EETP+TVKK   RP DAD    S 
Sbjct: 1   MDAMSSPFLSGGNLLLSPPISKSRTAVSTAICSVLAEETPRTVKKPASRP-DADFPSPSL 60

Query: 61  PLRATPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLAD 120
             RATPPP     A+AK SSTRRVEPS+PA +FNAFDDLINNFINPP NPS DPRYVLAD
Sbjct: 61  SQRATPPP-----AVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLAD 120

Query: 121 NFAPVDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDG 180
           NFAPVDELPPTECEVIQGSLP SLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+G
Sbjct: 121 NFAPVDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNG 180

Query: 181 RAVLCSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGI 240
           RAVLCSRYVKTYKYTLERD GHPVIPNVFSGFNGL ASAAR AVTAGRM TGQF+P NGI
Sbjct: 181 RAVLCSRYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGI 240

Query: 241 GLANTSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVT 300
           GLANTSLA+FGDRLYALGESDLPYPIRLTP GDIETL RHDFDGKLS+SMTAHPK+D  T
Sbjct: 241 GLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDT 300

Query: 301 GEAFAFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGF 360
           GEAFAFRYGPLPPFLTFFRFD++GAKQ DV IFSM+RPSFLHDFAIT+KYAVF EIQIGF
Sbjct: 301 GEAFAFRYGPLPPFLTFFRFDKNGAKQPDVRIFSMNRPSFLHDFAITKKYAVFTEIQIGF 360

Query: 361 NPMQMITEGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWDEDDAVV 420
           NPMQMI EGRSPVG+DPSK+ R+G+IPRYA DESKMKWFDVPG NL+HAINAWDEDDAV+
Sbjct: 361 NPMQMIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVI 420

Query: 421 MVAPNILSVEHTMERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVINPSYVGRKNR 480
           +VAPN+LSVEHTMERMDLVHALVEK+RIDLKTGI+TRRPLSTRNLDFGVINP +VG+KNR
Sbjct: 421 LVAPNVLSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLHVGKKNR 480

Query: 481 FVYAGVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREGENAGETAA 540
           FVYAGVGDPMPKISGVVKLDVSQEE RDCIVA+R+FGP CYGGEPFFVPRE EN+ +TAA
Sbjct: 481 FVYAGVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDKTAA 540

Query: 541 EEDDGYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESDLNKL 596
           EEDDGYVVSYVH+EN+G+SRF+VMDAKSP+L I+A VKLPRRVPYGFHGLFVKESDL+KL
Sbjct: 541 EEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

[Close]

BLAST of MC10g0801 vs. ExPASy TrEMBL
Match: K4JYD3 (Carotenoid cleavage dioxygenase 4 OS=Momordica charantia OX=3673 GN=CCD4 PE=2 SV=1)

HSP 1 Score: 1170 bits (3028), Expect = 0.0
Identity = 586/596 (98.32%), Postives = 588/596 (98.66%), Query Frame = 0


Query: 1   MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDADSSPLRA 60
           MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDADSSPLRA
Sbjct: 1   MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDADSSPLRA 60

Query: 61  TPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLADNFAP 120
           TPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLADNFAP
Sbjct: 61  TPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLADNFAP 120

Query: 121 VDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVL 180
           VDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVL
Sbjct: 121 VDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVL 180

Query: 181 CSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGIGLAN 240
           CSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGIGLAN
Sbjct: 181 CSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGIGLAN 240

Query: 241 TSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVTGEAF 300
           TSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVTGEAF
Sbjct: 241 TSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVTGEAF 300

Query: 301 AFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGFNPMQ 360
           AFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGE QIGFNPMQ
Sbjct: 301 AFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGETQIGFNPMQ 360

Query: 361 MITEGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWDEDDAVVMVAP 420
           MITEGRSPVGS+PSKVCR GIIPRYATDESKMKWFDVPGFNLIHAINAWDE DAVVMVAP
Sbjct: 361 MITEGRSPVGSNPSKVCRAGIIPRYATDESKMKWFDVPGFNLIHAINAWDEYDAVVMVAP 420

Query: 421 NILSVEHTMERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVINPSYVGRKNRFVYA 480
           NILSVEHTMERMDLVHALVEK RIDLKTGIVTRR LSTRNLDFGVINPSYVGRKNRFVYA
Sbjct: 421 NILSVEHTMERMDLVHALVEKARIDLKTGIVTRRSLSTRNLDFGVINPSYVGRKNRFVYA 480

Query: 481 GVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREGENAGETAAEEDD 540
           GVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPF VPREGENAGETAAEE D
Sbjct: 481 GVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFLVPREGENAGETAAEEGD 540

Query: 541 GYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESDLNKL 596
           GYVVSYVHNENTGESRFIVMDAKSP+LAIVAAVKLPRRVPYGF GLFVKESDLNKL
Sbjct: 541 GYVVSYVHNENTGESRFIVMDAKSPNLAIVAAVKLPRRVPYGFLGLFVKESDLNKL 596

[Close]

BLAST of MC10g0801 vs. ExPASy TrEMBL
Match: A0A346DA10 (Carotenoid cleavage dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111437641 PE=2 SV=1)

HSP 1 Score: 1036 bits (2678), Expect = 0.0
Identity = 512/600 (85.33%), Postives = 551/600 (91.83%), Query Frame = 0


Query: 1   MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDAD----SS 60
           MDAISSPFLSGGNLLLSP+IS SR +++T I SVL EETP+TVKK   RP DAD    S 
Sbjct: 1   MDAISSPFLSGGNLLLSPSISKSRTAVSTAICSVLAEETPRTVKKPASRP-DADFPSPSL 60

Query: 61  PLRATPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLAD 120
             RATPPP     A+AK SSTRRVEPS+PA +FNAFDDLINNFINPP NPS DPRYVLAD
Sbjct: 61  SQRATPPP-----AVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLAD 120

Query: 121 NFAPVDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDG 180
           NFAPVDELPPT+CEVIQGSLP SLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+G
Sbjct: 121 NFAPVDELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNG 180

Query: 181 RAVLCSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGI 240
           RAVLCSRYVKTYKYTLERD GHPVIPNVFSGFNGL ASAAR AVTAGRM TGQF+P NGI
Sbjct: 181 RAVLCSRYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPTNGI 240

Query: 241 GLANTSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVT 300
           GLANTSLA+FGDRLYALGESDLPYPIRLTP GDIETL RHDFDGKLS+SMTAHPK+D  T
Sbjct: 241 GLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDT 300

Query: 301 GEAFAFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGF 360
           GEAFAFRYGPLPPFLTFFRFD++GAKQ DVPIFSM+RPSFLHDFAIT+KYAVF EIQIGF
Sbjct: 301 GEAFAFRYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGF 360

Query: 361 NPMQMITEGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWDEDDAVV 420
           NPMQMI EGRSPVG+DPSK+ R+G+IPRYA DESKMKWFDVPG NL+HAINAWDEDDAV+
Sbjct: 361 NPMQMIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVI 420

Query: 421 MVAPNILSVEHTMERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVINPSYVGRKNR 480
           +VAPN+LSVEHTMERMDLVHALVEK+RIDLKTGI+TRRPLSTRNLDFGVINP YVG+KNR
Sbjct: 421 LVAPNVLSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLYVGKKNR 480

Query: 481 FVYAGVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREGENAGETAA 540
           FVYAGVGDPMPKISGVVKLDVSQEE RDCIVA+R+FGP CYGGEPFFVPRE EN+ E AA
Sbjct: 481 FVYAGVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDEMAA 540

Query: 541 EEDDGYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESDLNKL 596
           EEDDGYVVSYVH+EN+G+SRF+VMDAKSP+L I+A VKLPRRVPYGFHGLFVKESDL+KL
Sbjct: 541 EEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

[Close]

BLAST of MC10g0801 vs. ExPASy TrEMBL
Match: A0A346DA05 (Carotenoid cleavage dioxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 1035 bits (2676), Expect = 0.0
Identity = 513/600 (85.50%), Postives = 553/600 (92.17%), Query Frame = 0


Query: 1   MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDAD----SS 60
           MDAISSPFLSGGNLLLS  IS SR +++T I SVL EETP+TVKK   RP DAD    S 
Sbjct: 1   MDAISSPFLSGGNLLLSTPISKSRTAVSTAICSVLAEETPRTVKKPASRP-DADFPSPSL 60

Query: 61  PLRATPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLAD 120
             RATPPP     A+AK SSTRRVEPS+PA +FNAFDDLINNFINPPV+PSVDPRYVLAD
Sbjct: 61  SQRATPPP-----AVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPVSPSVDPRYVLAD 120

Query: 121 NFAPVDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDG 180
           NFAPVDELPPTECEVIQGSLP SLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+G
Sbjct: 121 NFAPVDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNG 180

Query: 181 RAVLCSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGI 240
           RAVLCSRYVKTYKYTLERD GHPV PNVFSGFNGLTASAAR AV AGR+LTGQ++PANGI
Sbjct: 181 RAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGI 240

Query: 241 GLANTSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVT 300
           GLANTS+A+FGDRLYALGESDLPYPIRLTP GDIETL RHDFDGKLS+SMTAHPK+D  T
Sbjct: 241 GLANTSVAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDT 300

Query: 301 GEAFAFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGF 360
           GEAFAFRYGPLPPFLTFFRFD++GAKQ DVPIFSM+RPSFLHDFAIT+KYAVF EIQIGF
Sbjct: 301 GEAFAFRYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGF 360

Query: 361 NPMQMITEGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWDEDDAVV 420
           NPMQMI EGRSPVG+DPSK+ R+G+IPRYA DESKMKWFDVPG NL+HAINAWDEDDAV+
Sbjct: 361 NPMQMIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVI 420

Query: 421 MVAPNILSVEHTMERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVINPSYVGRKNR 480
           +VAPNILSVEHTMERMDLVHALVEK+RIDLKTGI+TRRPLSTRNLDFGVINP YVG+KNR
Sbjct: 421 LVAPNILSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLYVGKKNR 480

Query: 481 FVYAGVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREGENAGETAA 540
           FVYAGVGDPMPKISGVVKLDVSQEE RDCIVA+R+FGP CYGGEPFFVPRE EN+ ETAA
Sbjct: 481 FVYAGVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDETAA 540

Query: 541 EEDDGYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESDLNKL 596
           EEDDGYVVSYVH+EN+G+SRF+VMDAKSP+L I+A VKLPRRVPYGFHGLFVKESDL+KL
Sbjct: 541 EEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

[Close]

BLAST of MC10g0801 vs. ExPASy TrEMBL
Match: A0A346DA08 (Carotenoid cleavage dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111483168 PE=2 SV=1)

HSP 1 Score: 1027 bits (2655), Expect = 0.0
Identity = 511/600 (85.17%), Postives = 549/600 (91.50%), Query Frame = 0


Query: 1   MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDAD----SS 60
           MDAISSPFLSGGNLLLSP  S  R +++T I SVLTEETP+TVKK   RP DAD    S 
Sbjct: 1   MDAISSPFLSGGNLLLSPPTSKYRTAVSTAICSVLTEETPRTVKKPASRP-DADFPSPSL 60

Query: 61  PLRATPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLAD 120
             RAT PP     A+AK SSTRRVEPS+PA +FNAFDDLINNFINPP NPS DPRYVLAD
Sbjct: 61  SQRATHPP-----AVAKGSSTRRVEPSVPASIFNAFDDLINNFINPPANPSTDPRYVLAD 120

Query: 121 NFAPVDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDG 180
           NFAPVDELPPTECEVIQGSLP SLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+G
Sbjct: 121 NFAPVDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNG 180

Query: 181 RAVLCSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGI 240
           RAVLCSRYVKTYKYTLERD GHPVIPNVFSGFNGL ASAAR AVTAGRM TGQF+PANGI
Sbjct: 181 RAVLCSRYVKTYKYTLERDAGHPVIPNVFSGFNGLIASAARGAVTAGRMFTGQFNPANGI 240

Query: 241 GLANTSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVT 300
           GLANTSLA+FGDRLYALGESDLPYPIRLTP GDIETL RHDFDGKLS+SMTAHPK+D  T
Sbjct: 241 GLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDT 300

Query: 301 GEAFAFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGF 360
           GEAFAFRYGPLPPFLTFFRFD++GAKQ DVPIFSM+RPSFLHDFAIT+KYAVF EIQIGF
Sbjct: 301 GEAFAFRYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGF 360

Query: 361 NPMQMITEGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWDEDDAVV 420
           NPMQMI EGRSPVG+DPSK+ R+G+IPRYA DESKMKWFDVPG NL+HAINAWDE+DAV+
Sbjct: 361 NPMQMIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEEDAVI 420

Query: 421 MVAPNILSVEHTMERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVINPSYVGRKNR 480
           +VAPNILSVEHTMERMDLVHALVEK+RIDLKTGI+TRRPLSTRNLDFGVINP YVG+KNR
Sbjct: 421 LVAPNILSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPVYVGKKNR 480

Query: 481 FVYAGVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREGENAGETAA 540
           FVYAGVGDPMPKISGVVKLDVSQEE RDCIVA+R+FGP  YGGEPFFVPRE E++ ETAA
Sbjct: 481 FVYAGVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNYYGGEPFFVPRERESSDETAA 540

Query: 541 EEDDGYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESDLNKL 596
           EEDDGYVVSYVH+EN+G+SRF+VMDAKSP+L I+A VKLPRRVPYGFHGLFVKESDL+KL
Sbjct: 541 EEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

[Close]

BLAST of MC10g0801 vs. ExPASy TrEMBL
Match: A0A6J1FTM6 (probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448011 PE=3 SV=1)

HSP 1 Score: 1013 bits (2618), Expect = 0.0
Identity = 504/596 (84.56%), Postives = 539/596 (90.44%), Query Frame = 0


Query: 1   MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDADSSPLRA 60
           MDA+SSPFL+GGNLLLSP IS SRP  +  I+SV TEETPKTVK+T     +ADS   R 
Sbjct: 1   MDALSSPFLTGGNLLLSPPISTSRPPFSMAITSVFTEETPKTVKRT-----NADSPSPRR 60

Query: 61  TPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLADNFAP 120
            PPP     AMAK  ST R+EPSLPAR FNAFDDLINNFINPPV+PSVDPRYVLADNFAP
Sbjct: 61  PPPP-----AMAKGPSTTRLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAP 120

Query: 121 VDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVL 180
           VDELPPTECEVIQGSLP SLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+GRAVL
Sbjct: 121 VDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVL 180

Query: 181 CSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGIGLAN 240
           CSRYVKTYKYTLERD GHPV PNVFSGFNGLTASAAR AV AGR+LTGQ++PANGIGLAN
Sbjct: 181 CSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLAN 240

Query: 241 TSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVTGEAF 300
           TS+A+FGD LYALGESDLPY IRL P G+IETL R DFDG L +SMTAHPK DP TGE F
Sbjct: 241 TSVAFFGDSLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETF 300

Query: 301 AFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGFNPMQ 360
           AFRYGPLPPFLTFFRFD++GAKQSDVPIFSM+RPSFLHDFAIT+KYAVF EIQIGFNPM 
Sbjct: 301 AFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPML 360

Query: 361 MITEGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWDEDDAVVMVAP 420
           MI EGRSPVG+DPS V RVG+IPRYA DESKMKWFDVPGFNLIHAINAWDEDDAVV++AP
Sbjct: 361 MIMEGRSPVGTDPSTVSRVGLIPRYADDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAP 420

Query: 421 NILSVEHTMERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVINPSYVGRKNRFVYA 480
           NILSVEHT+ERMDLVHAL+E+VRIDLKTGIVTRRPLSTRNLDFGVINPSY+G+KNRFVYA
Sbjct: 421 NILSVEHTLERMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFVYA 480

Query: 481 GVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREGENAGETAAEEDD 540
           GVGDPMPKISGVVKLDVSQEE RDCIVASRIFGPGCYGGEPFFVPRE E++ ETA EEDD
Sbjct: 481 GVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDD 540

Query: 541 GYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESDLNKL 596
           GYVVSYVH+EN+GES+FIVMDAKSP L I+A VKLPRRV YGFHGLFVKESDL KL
Sbjct: 541 GYVVSYVHDENSGESKFIVMDAKSPKLEIIAVVKLPRRVSYGFHGLFVKESDLRKL 586

[Close]

BLAST of MC10g0801 vs. TAIR 10
Match: AT4G19170.1 (nine-cis-epoxycarotenoid dioxygenase 4 )

HSP 1 Score: 795.0 bits (2052), Expect = 4.0e-230
Identity = 389/604 (64.40%), Postives = 480/604 (79.47%), Query Frame = 0


Query: 1   MDAISSPFLSGGNLLLSPAI---SISRPSIATVISSVLTEETPKTVKKTGPRPSDADSSP 60
           MD++SS         L  ++     S P++  + S+V+ E +P T       PSD +   
Sbjct: 1   MDSVSSSSFLSSTFSLHHSLLRRRSSSPTLLRINSAVVEERSPIT------NPSDNNDRR 60

Query: 61  LRATPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLADN 120
            +           +  S    R E +L   LF   +D+IN FI+PP  PSVDP++VL+DN
Sbjct: 61  NKPKTLHNRTNHTLVSSPPKLRPEMTLATALFTTVEDVINTFIDPPSRPSVDPKHVLSDN 120

Query: 121 FAPV-DELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDG 180
           FAPV DELPPT+CE+I G+LP SLNGAYIRNGPNPQ+LPRGPYHLFDGDGMLH+++I +G
Sbjct: 121 FAPVLDELPPTDCEIIHGTLPLSLNGAYIRNGPNPQFLPRGPYHLFDGDGMLHAIKIHNG 180

Query: 181 RAVLCSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGI 240
           +A LCSRYVKTYKY +E+  G PV+PNVFSGFNG+TAS AR A+TA R+LTGQ++P NGI
Sbjct: 181 KATLCSRYVKTYKYNVEKQTGAPVMPNVFSGFNGVTASVARGALTAARVLTGQYNPVNGI 240

Query: 241 GLANTSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVT 300
           GLANTSLA+F +RL+ALGESDLPY +RLT +GDIET+ R+DFDGKL++SMTAHPK DP+T
Sbjct: 241 GLANTSLAFFSNRLFALGESDLPYAVRLTESGDIETIGRYDFDGKLAMSMTAHPKTDPIT 300

Query: 301 GEAFAFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIG- 360
           GE FAFRYGP+PPFLT+FRFD +G KQ DVPIFSM+ PSFLHDFAIT+++A+F EIQ+G 
Sbjct: 301 GETFAFRYGPVPPFLTYFRFDSAGKKQRDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGM 360

Query: 361 -FNPMQMITEGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWDEDD- 420
             N + ++ EG SPVG+D  K  R+G+IP+YA DES+MKWF+VPGFN+IHAINAWDEDD 
Sbjct: 361 RMNMLDLVLEGGSPVGTDNGKTPRLGVIPKYAGDESEMKWFEVPGFNIIHAINAWDEDDG 420

Query: 421 -AVVMVAPNILSVEHTMERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVINPSYVG 480
            +VV++APNI+S+EHT+ERMDLVHALVEKV+IDL TGIV R P+S RNLDF VINP+++G
Sbjct: 421 NSVVLIAPNIMSIEHTLERMDLVHALVEKVKIDLVTGIVRRHPISARNLDFAVINPAFLG 480

Query: 481 RKNRFVYAGVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREGENAG 540
           R +R+VYA +GDPMPKISGVVKLDVS+ +  DC VA R++G GCYGGEPFFV R+    G
Sbjct: 481 RCSRYVYAAIGDPMPKISGVVKLDVSKGDRDDCTVARRMYGSGCYGGEPFFVARD---PG 540

Query: 541 ETAAEEDDGYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESD 597
              AEEDDGYVV+YVH+E TGES+F+VMDAKSP+L IVAAV+LPRRVPYGFHGLFVKESD
Sbjct: 541 NPEAEEDDGYVVTYVHDEVTGESKFLVMDAKSPELEIVAAVRLPRRVPYGFHGLFVKESD 595

[Close]

BLAST of MC10g0801 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 391.0 bits (1003), Expect = 1.8e-108
Identity = 230/607 (37.89%), Postives = 339/607 (55.85%), Query Frame = 0


Query: 3   AISSPFLSGGNLLLSPAISISRPSIATVISS--VLTEETPKTVKKTGPRPSDADSSPLRA 62
           A+S  +L G +    P +S S+ S  +  SS  + +  T K    +      A   P ++
Sbjct: 9   AVSGRWLGGNH--TQPPLSSSQSSDLSYCSSLPMASRVTRKLNVSSALHTPPALHFPKQS 68

Query: 63  TPPPPAKVPAMAKSSSTRRV---EPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLADN 122
           +  P   V   AK S+T+++   + +  A L  A   L+++    P+  + DP   +A N
Sbjct: 69  SNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGN 128

Query: 123 FAPVDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGR 182
           FAPV+E P      + G LP S+ G Y+RNG NP + P   +H FDGDGM+H+++   G 
Sbjct: 129 FAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKFEHGS 188

Query: 183 AVLCSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGIG 242
           A    R+ +T ++  ER +G PV P      +G T   AR  +   R   G  DPA+G G
Sbjct: 189 ASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHT-GIARLMLFYARAAAGIVDPAHGTG 248

Query: 243 LANTSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVTG 302
           +AN  L YF  RL A+ E DLPY +++TP GD++T+ R DFDG+L  +M AHPKVDP +G
Sbjct: 249 VANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESG 308

Query: 303 EAFAFRYGPL-PPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGF 362
           E FA  Y  +  P+L +FRF   G K  DV I  + +P+ +HDFAITE + V  + Q+ F
Sbjct: 309 ELFALSYDVVSKPYLKYFRFSPDGTKSPDVEI-QLDQPTMMHDFAITENFVVVPDQQVVF 368

Query: 363 NPMQMITEGRSPVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWD--EDDA 422
              +MI  G SPV  D +KV R GI+ +YA D S +KW D P     H  NAW+  E D 
Sbjct: 369 KLPEMI-RGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDE 428

Query: 423 VVMVAPNILSVEHTMERMD-LVHALVEKVRIDLKTGIVTRRPLSTR-----NLDFGVINP 482
           VV++   +   +      D  + +++ ++R++LKTG  TRRP+ +      NL+ G++N 
Sbjct: 429 VVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNR 488

Query: 483 SYVGRKNRFVYAGVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREG 542
           + +GRK +F Y  + +P PK+SG  K+D++  E     V   ++G   YGGEP F+P EG
Sbjct: 489 NMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGE-----VKKHLYGDNRYGGEPLFLPGEG 548

Query: 543 ENAGETAAEEDDGYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFV 596
                   EED+GY++ +VH+E T +S   +++A S  L + A VKLP RVPYGFHG F+
Sbjct: 549 -------GEEDEGYILCFVHDEKTWKSELQIVNAVS--LEVEATVKLPSRVPYGFHGTFI 596

[Close]

BLAST of MC10g0801 vs. TAIR 10
Match: AT3G63520.1 (carotenoid cleavage dioxygenase 1 )

HSP 1 Score: 382.1 bits (980), Expect = 8.2e-106
Identity = 210/542 (38.75%), Postives = 304/542 (56.09%), Query Frame = 0


Query: 71  MAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLADNFAPV-DELPPTEC 130
           ++  SS   V P       +   DL+   +   ++ +  P + L+ NFAP+ DE PP + 
Sbjct: 5   LSDGSSIISVHPRPSKGFSSKLLDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKD 64

Query: 131 EVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYVKTYK 190
             + G LP  LNG ++R GPNP++     YH FDGDGM+H +RI DG+A   SRYVKT +
Sbjct: 65  LPVHGFLPECLNGEFVRVGPNPKFDAVAGYHWFDGDGMIHGVRIKDGKATYVSRYVKTSR 124

Query: 191 YTLERDVGHPVIPNV--FSGFNGLTASAARSAVTAGRMLTGQFDPANGIGLANTSLAYFG 250
              E   G      +    GF GL     +   T  ++L    D   G G ANT+L Y  
Sbjct: 125 LKQEEFFGAAKFMKIGDLKGFFGLLMVNVQQLRTKLKIL----DNTYGNGTANTALVYHH 184

Query: 251 DRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVTGEAFAFRYGPL 310
            +L AL E+D PY I++   GD++TL   D+D +L+ S TAHPKVDPVTGE F F Y   
Sbjct: 185 GKLLALQEADKPYVIKVLEDGDLQTLGIIDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHT 244

Query: 311 PPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGFNPMQMITEGRS 370
           PP+LT+    + G     VPI ++S P  +HDFAITE YA+F ++ + F P +M+ E + 
Sbjct: 245 PPYLTYRVISKDGIMHDPVPI-TISEPIMMHDFAITETYAIFMDLPMHFRPKEMVKEKKM 304

Query: 371 PVGSDPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWDEDDAVVMVAPNILSVEH 430
               DP+K  R G++PRYA DE  ++WF++P   + H  NAW+E+D VV++   + + + 
Sbjct: 305 IYSFDPTKKARFGVLPRYAKDELMIRWFELPNCFIFHNANAWEEEDEVVLITCRLENPDL 364

Query: 431 TM------ERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVINPSYVGRKNRFVYAG 490
            M      E+++     + ++R ++KTG  +++ LS   +DF  IN  Y G+K R+VY  
Sbjct: 365 DMVSGKVKEKLENFGNELYEMRFNMKTGSASQKKLSASAVDFPRINECYTGKKQRYVYGT 424

Query: 491 VGDPMPKISGVVKLDVSQE--------ECRDCIVASRIFGPGCYGGEPFFVPREGENAGE 550
           + D + K++G++K D+  E        E    I      G G YG E  +VPRE      
Sbjct: 425 ILDSIAKVTGIIKFDLHAEAETGKRMLEVGGNIKGIYDLGEGRYGSEAIYVPRE------ 484

Query: 551 TAAEEDDGYVVSYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESDL 596
             AEEDDGY++ +VH+ENTG+S   V+DAK+     VA V+LP RVPYGFH LFV E  L
Sbjct: 485 -TAEEDDGYLIFFVHDENTGKSCVTVIDAKTMSAEPVAVVELPHRVPYGFHALFVTEEQL 534

[Close]

BLAST of MC10g0801 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 379.8 bits (974), Expect = 4.0e-105
Identity = 220/589 (37.35%), Postives = 332/589 (56.37%), Query Frame = 0


Query: 13  NLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDADSSPLRATPPPPAKVPAMA 72
           NL  +P+  +  PS ++ +S   T   P+  K +    SD  +       P P  +    
Sbjct: 15  NLSFAPS-DLDAPSPSSSVS--FTNTKPRRRKLSANSVSDTPNLLNFPNYPSPNPIIPEK 74

Query: 73  KSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLADNFAPVDELPPTECEVI 132
            +S    ++ +  A L  A   L+    + P+  +VDPR+ ++ N+APV E        +
Sbjct: 75  DTSRWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSV 134

Query: 133 QGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYVKTYKYTL 192
            G +P  ++G Y+RNG NP + P   +HLFDGDGM+H+++I++G A    R+ +T +   
Sbjct: 135 DGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQ 194

Query: 193 ERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGIGLANTSLAYFGDRLYA 252
           E+ +G P+ P      +G  +  AR  +   R L G  +  NG G+AN  L YF DRL A
Sbjct: 195 EKQLGSPIFPKAIGELHG-HSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLA 254

Query: 253 LGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVTGEAFAFRYGPL-PPFL 312
           + E DLPY +R+T  GD+ET+ R DFDG+LS +M AHPK+DPVT E FA  Y  +  P+L
Sbjct: 255 MSEDDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYL 314

Query: 313 TFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGEIQIGFNPMQMITEGRSPVGS 372
            +F+F   G K  DV I  ++ P+ +HDFAITE + V  + Q+ F    M   G+SPV  
Sbjct: 315 KYFKFSPEGEKSPDVEI-PLASPTMMHDFAITENFVVIPDQQVVFKLSDMFL-GKSPVKY 374

Query: 373 DPSKVCRVGIIPRYATDESKMKWFDVPGFNLIHAINAWD--EDDAVVMVAPNILSVEHTM 432
           D  K+ R GI+PR A D S+M W + P     H  NAW+  E D VV++   +   +   
Sbjct: 375 DGEKISRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIF 434

Query: 433 ERMD-LVHALVEKVRIDLKTGIVTRRPL----STRNLDFGVINPSYVGRKNRFVYAGVGD 492
              D  +++++ ++R++LKTG  TRR +       NL+ G++N + +GRK R+ Y  + +
Sbjct: 435 NECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAE 494

Query: 493 PMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFFVPREGENAGETAAEEDDGYVV 552
           P PK+SG  K+D+S  E     V +  +G   YGGEPFF+PR  E+ G     EDDGY++
Sbjct: 495 PWPKVSGFAKVDLSTGE-----VKNHFYGGKKYGGEPFFLPRGLESDG-----EDDGYIM 554

Query: 553 SYVHNENTGESRFIVMDAKSPDLAIVAAVKLPRRVPYGFHGLFVKESDL 594
           S+VH+E + ES   +++A +  L + A VKLP RVPYGFHG FV  +D+
Sbjct: 555 SFVHDEESWESELHIVNAVT--LELEATVKLPSRVPYGFHGTFVNSADM 585

[Close]

BLAST of MC10g0801 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 372.1 bits (954), Expect = 8.4e-103
Identity = 196/506 (38.74%), Postives = 302/506 (59.68%), Query Frame = 0


Query: 95  LINNFINPPVNPSVDPRYVLADNFAPVDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYL 154
           LI++  + P+  + DPR  +A N++PV E        ++G++P  ++G YIRNG NP + 
Sbjct: 87  LISHEQDSPLPKTADPRVQIAGNYSPVPESSVRRNLTVEGTIPDCIDGVYIRNGANPMFE 146

Query: 155 PRGPYHLFDGDGMLHSLRISDGRAVLCSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTAS 214
           P   +HLFDGDGM+H+++I++G A    R+ KT +   E+ +G PV P      +G  + 
Sbjct: 147 PTAGHHLFDGDGMVHAVKITNGSASYACRFTKTERLVQEKRLGRPVFPKAIGELHG-HSG 206

Query: 215 AARSAVTAGRMLTGQFDPANGIGLANTSLAYFGDRLYALGESDLPYPIRLTPTGDIETLD 274
            AR  +   R L G  +  NG+G+AN  L YF +RL A+ E DLPY +++T TGD++T+ 
Sbjct: 207 IARLMLFYARGLCGLINNQNGVGVANAGLVYFNNRLLAMSEDDLPYQLKITQTGDLQTVG 266

Query: 275 RHDFDGKLSISMTAHPKVDPVTGEAFAFRYGPL-PPFLTFFRFDRSGAKQSDVPIFSMSR 334
           R+DFDG+L  +M AHPK+DPVT E  A  Y  +  P+L +FRF   G K  ++ I  +  
Sbjct: 267 RYDFDGQLKSAMIAHPKLDPVTKELHALSYDVVKKPYLKYFRFSPDGVKSPELEI-PLET 326

Query: 335 PSFLHDFAITEKYAVFGEIQIGFNPMQMITEGRSPVGSDPSKVCRVGIIPRYATDESKMK 394
           P+ +HDFAITE + V  + Q+ F   +MI+ G+SPV  D  KV R+GI+P+ AT+ S++ 
Sbjct: 327 PTMIHDFAITENFVVIPDQQVVFKLGEMIS-GKSPVVFDGEKVSRLGIMPKDATEASQII 386

Query: 395 WFDVPGFNLIHAINAWD--EDDAVVMVAPNILSVEHTM-ERMDLVHALVEKVRIDLKTGI 454
           W + P     H  NAW+  E + +V++   +   +    ER + + +++ ++RI+L+T  
Sbjct: 387 WVNSPETFCFHLWNAWESPETEEIVVIGSCMSPADSIFNERDESLRSVLSEIRINLRTRK 446

Query: 455 VTRRPLSTR---NLDFGVINPSYVGRKNRFVYAGVGDPMPKISGVVKLDVSQEECRDCIV 514
            TRR L      NL+ G++N + +GRK RF +  +  P PK+SG  K+D+   E +    
Sbjct: 447 TTRRSLLVNEDVNLEIGMVNRNRLGRKTRFAFLAIAYPWPKVSGFAKVDLCTGEMK---- 506

Query: 515 ASRIFGPGCYGGEPFFVPREGENAGETAAEEDDGYVVSYVHNENTGESRFIVMDAKSPDL 574
              I+G   YGGEPFF+P    N+G     EDDGY+  +VH+E T  S   +++A   +L
Sbjct: 507 -KYIYGGEKYGGEPFFLP---GNSGNGEENEDDGYIFCHVHDEETKTSELQIINA--VNL 566

Query: 575 AIVAAVKLPRRVPYGFHGLFVKESDL 594
            + A +KLP RVPYGFHG FV  ++L
Sbjct: 567 KLEATIKLPSRVPYGFHGTFVDSNEL 579

The following BLAST results are available for this feature:

BLAST of MC10g0801 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49675	5.7e-229	64.40	Probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Arabidopsis thalian...	[more]
Q6YVJ0	4.4e-197	59.29	9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Oryza sativa subsp. ja...	[more]
Q5MBR6	8.9e-166	56.60	9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Oryza sativa subsp. ja...	[more]
Q84K96	2.5e-120	57.72	Zeaxanthin 7,8(7',8')-cleavage dioxygenase, chromoplastic OS=Crocus sativus OX=8...	[more]
Q9LRR7	2.5e-107	37.89	9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O...	[more]

BLAST of MC10g0801 vs. NCBI nr


Match Name	Identity	Description
AFU91490.1	98.32	carotenoid cleavage dioxygenase 4 [Momordica charantia]	[more]
XP_038897022.1	87.94	probable carotenoid cleavage dioxygenase 4, chloroplastic [Benincasa hispida]	[more]
XP_022931473.1	85.33	probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita moschata] >...	[more]
AXM43896.1	85.50	carotenoid cleavage dioxygenase [Cucurbita pepo]	[more]
KAG7015104.1	85.00	putative carotenoid cleavage dioxygenase 4, chloroplastic, partial [Cucurbita ar...	[more]

BLAST of MC10g0801 vs. ExPASy TrEMBL


Match Name	Identity	Description
K4JYD3	98.32	Carotenoid cleavage dioxygenase 4 OS=Momordica charantia OX=3673 GN=CCD4 PE=2 SV...	[more]
A0A346DA10	85.33	Carotenoid cleavage dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111437641 PE...	[more]
A0A346DA05	85.50	Carotenoid cleavage dioxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1	[more]
A0A346DA08	85.17	Carotenoid cleavage dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111483168 PE=2...	[more]
A0A6J1FTM6	84.56	probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Cucurbita moschata ...	[more]

BLAST of MC10g0801 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19170.1	4.0e-230	64.40	nine-cis-epoxycarotenoid dioxygenase 4	[more]
AT3G14440.1	1.8e-108	37.89	nine-cis-epoxycarotenoid dioxygenase 3	[more]
AT3G63520.1	8.2e-106	38.75	carotenoid cleavage dioxygenase 1	[more]
AT1G30100.1	4.0e-105	37.35	nine-cis-epoxycarotenoid dioxygenase 5	[more]
AT4G18350.1	8.4e-103	38.74	nine-cis-epoxycarotenoid dioxygenase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004294	Carotenoid oxygenase	PFAM	PF03055	RPE65	coord: 119..588 e-value: 2.3E-109 score: 366.6
IPR004294	Carotenoid oxygenase	PANTHER	PTHR10543	BETA-CAROTENE DIOXYGENASE	coord: 49..595
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 37..79
None	No IPR available	PANTHER	PTHR10543:SF46	CAROTENOID CLEAVAGE DIOXYGENASE 4, CHLOROPLASTIC-RELATED	coord: 49..595

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g0801.1	MC10g0801.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016121	carotene catabolic process
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0010436	carotenoid dioxygenase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen