Homology
BLAST of MC10g0778 vs. ExPASy Swiss-Prot
Match:
Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)
HSP 1 Score: 343.2 bits (879), Expect = 8.2e-93
Identity = 189/415 (45.54%), Postives = 258/415 (62.17%), Query Frame = 0
Query: 427 GFTTSLFHRDSLLSPLRRPSLSHYDRLHHAFRRSFARSAALFNRASAVASPAAIQSPLTP 486
GFT L HRDS SP P + RL +A RS R +F+ +P Q LT
Sbjct: 30 GFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNR---VFHFTEKDNTPQP-QIDLTS 89
Query: 487 GSGEYLIEVSIGTPPVDFLAIADTGSDLVWTQCLPCRKCFNQSLPIFDPRRSSSFRHVAC 546
SGEYL+ VSIGTPP +AIADTGSDL+WTQC PC C+ Q P+FDP+ SS+++ V+C
Sbjct: 90 NSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKTSSTYKDVSC 149
Query: 547 TSDPCRILD-VSRCGAH-QICDYSYTYGDQSYTKGELGFDKITLGS-----FPLSKTVVG 606
+S C L+ + C + C YS +YGD SYTKG + D +TLGS L ++G
Sbjct: 150 SSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSSDTRPMQLKNIIIG 209
Query: 607 CGHESDGGFGDIASGVIGLGGGPLSLVSQLNKYSNVTRRFSYCLPNVYSQAKLTAKINFG 666
CGH + G F SG++GLGGGP+SL+ QL ++ +FSYCL + S+ T+KINFG
Sbjct: 210 CGHNNAGTFNKKGSGIVGLGGGPVSLIKQLG--DSIDGKFSYCLVPLTSKKDQTSKINFG 269
Query: 667 GNAVVSGSGVVSTPFVPKL-PVTYYYITLEAVSVGNQRHEAAAKMSFAA-GNMIIDSGTT 726
NA+VSGSGVVSTP + K T+YY+TL+++SVG+++ + + S ++ GN+IIDSGTT
Sbjct: 270 TNAIVSGSGVVSTPLIAKASQETFYYLTLKSISVGSKQIQYSGSDSESSEGNIIIDSGTT 329
Query: 727 LTFLPQELYDSVVSSLVRVVRARRVEDPGGVLGLCFAAEEGRGGVDFPTITAHFSGGADV 786
LT LP E Y + ++ + A + +DP L LC++A G + P IT HF GADV
Sbjct: 330 LTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCYSA---TGDLKVPVITMHFD-GADV 389
Query: 787 RLPAVNTFERVADDVSCLTMASSSDFGILGNLAQMNFLIGYDMEAMRLSFKSTVC 833
+L + N F +V++D+ C S F I GN+AQMNFL+GYD + +SFK T C
Sbjct: 390 KLDSSNAFVQVSEDLVCFAFRGSPSFSIYGNVAQMNFLVGYDTVSKTVSFKPTDC 434
BLAST of MC10g0778 vs. ExPASy Swiss-Prot
Match:
Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)
HSP 1 Score: 332.0 bits (850), Expect = 1.9e-89
Identity = 187/442 (42.31%), Postives = 273/442 (61.76%), Query Frame = 0
Query: 413 LSFKPTVSTTYGRGGFTTSLFHRDSLLSPLRRPSLSHYDRLHHAFRRSFARSAALFNRAS 472
L F T+S++ F+ L HRDS LSP+ P ++ DRL+ AF RS +RS R +
Sbjct: 11 LFFSVTLSSSGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRS----RRFN 70
Query: 473 AVASPAAIQSPLTPGSGEYLIEVSIGTPPVDFLAIADTGSDLVWTQCLPCRKCFNQSLPI 532
S +QS L GE+ + ++IGTPP+ AIADTGSDL W QC PC++C+ ++ PI
Sbjct: 71 HQLSQTDLQSGLIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENGPI 130
Query: 533 FDPRRSSSFRHVACTSDPCRILDVSRCG---AHQICDYSYTYGDQSYTKGELGFDKITLG 592
FD ++SS+++ C S C+ L + G ++ IC Y Y+YGDQS++KG++ + +++
Sbjct: 131 FDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVSID 190
Query: 593 S---FPLS--KTVVGCGHESDGGFGDIASGVIGLGGGPLSLVSQLNKYSNVTRRFSYCLP 652
S P+S TV GCG+ + G F + SG+IGLGGG LSL+SQL S+++++FSYCL
Sbjct: 191 SASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLG--SSISKKFSYCLS 250
Query: 653 NVYSQAKLTAKINFGGNAVVSG----SGVVSTPFVPKLPVTYYYITLEAVSVGNQRHEAA 712
+ + T+ IN G N++ S SGVVSTP V K P+TYYY+TLEA+SVG ++
Sbjct: 251 HKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLTLEAISVGKKKIPYT 310
Query: 713 AK---------MSFAAGNMIIDSGTTLTFLPQELYDSVVSSLVR-VVRARRVEDPGGVLG 772
+S +GN+IIDSGTTLT L +D S++ V A+RV DP G+L
Sbjct: 311 GSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQGLLS 370
Query: 773 LCFAAEEGRGGVDFPTITAHFSGGADVRLPAVNTFERVADDVSCLTMASSSDFGILGNLA 832
CF + G + P IT HF+ GADVRL +N F ++++D+ CL+M +++ I GN A
Sbjct: 371 HCF--KSGSAEIGLPEITVHFT-GADVRLSPINAFVKLSEDMVCLSMVPTTEVAIYGNFA 430
BLAST of MC10g0778 vs. ExPASy Swiss-Prot
Match:
Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)
HSP 1 Score: 246.1 bits (627), Expect = 1.4e-63
Identity = 154/413 (37.29%), Postives = 214/413 (51.82%), Query Frame = 0
Query: 427 GFTTSLFHRDSLLSPLRRPSLSHYDRLHHAFRRSFARSAALFNRASAVASPAAIQSPLTP 486
GF L H DS +L+ + L A R R L + + P+ +++ +
Sbjct: 40 GFQIMLEHVDS------GKNLTKFQLLERAIERGSRRLQRL---EAMLNGPSGVETSVYA 99
Query: 487 GSGEYLIEVSIGTPPVDFLAIADTGSDLVWTQCLPCRKCFNQSLPIFDPRRSSSFRHVAC 546
G GEYL+ +SIGTP F AI DTGSDL+WTQC PC +CFNQS PIF+P+ SSSF + C
Sbjct: 100 GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPC 159
Query: 547 TSDPCRILDVSRCGAHQICDYSYTYGDQSYTKGELGFDKITLGSFPLSKTVVGCGHESDG 606
+S C+ L C ++ C Y+Y YGD S T+G +G + +T GS + GCG E++
Sbjct: 160 SSQLCQALSSPTC-SNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGCG-ENNQ 219
Query: 607 GFGD-IASGVIGLGGGPLSLVSQLNKYSNVTRRFSYCLPNVYSQAKLTAKINFGGNAVVS 666
GFG +G++G+G GPLSL SQL+ +FSYC+ + S + N+V +
Sbjct: 220 GFGQGNGAGLVGMGRGPLSLPSQLD-----VTKFSYCMTPIGSSTPSNLLLGSLANSVTA 279
Query: 667 GSGVVSTPFVPKLPVTYYYITLEAVSVGNQR-----HEAAAKMSFAAGNMIIDSGTTLTF 726
GS + ++P T+YYITL +SVG+ R A + G +IIDSGTTLT+
Sbjct: 280 GSPNTTLIQSSQIP-TFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTY 339
Query: 727 LPQELYDSVVSSLVRVVRARRVEDPGGVLGLCFAAEEGRGGVDFPTITAHFSGGADVRLP 786
Y SV + + V LCF + PT HF GG D+ LP
Sbjct: 340 FVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGG-DLELP 399
Query: 787 AVNTFERVADDVSCLTMASSSD-FGILGNLAQMNFLIGYDMEAMRLSFKSTVC 833
+ N F ++ + CL M SSS I GN+ Q N L+ YD +SF S C
Sbjct: 400 SENYFISPSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434
BLAST of MC10g0778 vs. ExPASy Swiss-Prot
Match:
Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)
HSP 1 Score: 233.8 bits (595), Expect = 7.0e-60
Identity = 142/393 (36.13%), Postives = 204/393 (51.91%), Query Frame = 0
Query: 446 SLSHYDRLHHAFRRSFARSAALFNRASAVASPAAIQSPLTPGSGEYLIEVSIGTPPVDFL 505
+L+ Y+ + A +R R ++ + + S + I++P+ G GEYL+ V+IGTP F
Sbjct: 54 NLTKYELIKRAIKRGERRMRSI---NAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFS 113
Query: 506 AIADTGSDLVWTQCLPCRKCFNQSLPIFDPRRSSSFRHVACTSDPCRILDVSRCGAHQIC 565
AI DTGSDL+WTQC PC +CF+Q PIF+P+ SSSF + C S C+ L C ++ C
Sbjct: 114 AIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNNNE-C 173
Query: 566 DYSYTYGDQSYTKGELGFDKITLGSFPLSKTVVGCGHESDGGFGDIASGVIGLGGGPLSL 625
Y+Y YGD S T+G + + T + + GCG ++ G +G+IG+G GPLSL
Sbjct: 174 QYTYGYGDGSTTQGYMATETFTFETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSL 233
Query: 626 VSQLNKYSNVTRRFSYCLPNVYSQAKLTAKINFGGNAVVSGSGVVSTPFVPKLPVTYYYI 685
SQL +FSYC+ + S + T + + V GS +T L TYYYI
Sbjct: 234 PSQLG-----VGQFSYCMTSYGSSSPSTLALGSAASGVPEGS-PSTTLIHSSLNPTYYYI 293
Query: 686 TLEAVSVGNQR----HEAAAKMSFAAGNMIIDSGTTLTFLPQELYDSVVSSLVRVVRARR 745
TL+ ++VG G MIIDSGTTLT+LPQ+ Y++V + +
Sbjct: 294 TLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPT 353
Query: 746 VEDPGGVLGLCFAAEEGRGGVDFPTITAHFSGGADVRLPAVNTFERVADDVSCLTMASSS 805
V++ L CF V P I+ F GG + L N A+ V CL M SSS
Sbjct: 354 VDESSSGLSTCFQQPSDGSTVQVPEISMQFDGGV-LNLGEQNILISPAEGVICLAMGSSS 413
Query: 806 DFG--ILGNLAQMNFLIGYDMEAMRLSFKSTVC 833
G I GN+ Q + YD++ + +SF T C
Sbjct: 414 QLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435
BLAST of MC10g0778 vs. ExPASy Swiss-Prot
Match:
Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)
HSP 1 Score: 203.8 bits (517), Expect = 7.8e-51
Identity = 142/433 (32.79%), Postives = 221/433 (51.04%), Query Frame = 0
Query: 11 SEATTHSTNGFTASLFHRD---SSLSPYDRLNNAVRRSFSRSATLFRRAA--AGVSTTAA 70
S + + S++ T +L H D S+ +P + ++ ++R R ++ AA G + T A
Sbjct: 62 SGSDSESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHA 121
Query: 71 KFP----------IIPANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSG 130
P + +GE+ + +GTP + DTGSD+ W QC PC +C++QS
Sbjct: 122 PRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSD 181
Query: 131 PIFNPRGSSTYRQVSCRSGTCHSLQASGCGPDGRTCGYGYTYGDQSYTYGELGRDKITIG 190
PIF+PR S TY + C S C L ++GC +TC Y +YGD S+T G+ + +T
Sbjct: 182 PIFDPRKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFR 241
Query: 191 SFRLRNTVIGCGHENGGTFGSGASSGIIGLGGGSLSLVSQLNKIGAVRRRFSYCLPNIFR 250
R++ +GCGH+N G F A G++GLG G LS Q ++FSYCL + R
Sbjct: 242 RNRVKGVALGCGHDNEGLFVGAA--GLLGLGKGKLSFPGQTGH--RFNQKFSYCL--VDR 301
Query: 251 NANLTGTINFGGHAVVSGRGAVSTPLVPKNP--DTYYYLTLEAVSVGNTR---HAADMSS 310
+A+ + G+A VS R A TPL+ NP DT+YY+ L +SVG TR A +
Sbjct: 302 SASSKPSSVVFGNAAVS-RIARFTPLL-SNPKLDTFYYVGLLGISVGGTRVPGVTASLFK 361
Query: 311 AVEGGMGNIIIDSGTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYATNEIRDG 370
+ G G +IIDSGT++T L + Y + + + + + C+ + + +
Sbjct: 362 LDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEV 421
Query: 371 HIPTITAHFGGC-----ASVKLLPLNTFGEVAENVSCLTFAPS-SELAIFGNLAQMNFLV 418
+PT+ HF G A+ L+P++T G+ C FA + L+I GN+ Q F V
Sbjct: 422 KVPTVVLHFRGADVSLPATNYLIPVDTNGKF-----CFAFAGTMGGLSIIGNIQQQGFRV 481
BLAST of MC10g0778 vs. NCBI nr
Match:
XP_022136655.1 (aspartic proteinase CDR1-like [Momordica charantia])
HSP 1 Score: 833 bits (2152), Expect = 1.27e-297
Identity = 416/419 (99.28%), Postives = 417/419 (99.52%), Query Frame = 0
Query: 1 VFFILLFFSFSEATTHSTNGFTASLFHRDSSLSPYDRLNNAVRRSFSRSATLFRRAAAGV 60
VFFILLFFSFSEATTHSTNGFTASLFHRDSSLSPYDRLNNAVRRSFSRSATLFRRAAAGV
Sbjct: 6 VFFILLFFSFSEATTHSTNGFTASLFHRDSSLSPYDRLNNAVRRSFSRSATLFRRAAAGV 65
Query: 61 STTAAKFPIIPANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSGPIFNP 120
STTAAKFPIIPANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSGPIFNP
Sbjct: 66 STTAAKFPIIPANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSGPIFNP 125
Query: 121 RGSSTYRQVSCRSGTCHSLQASGCGPDGRTCGYGYTYGDQSYTYGELGRDKITIGSFRLR 180
RGSSTYRQVSCRSGTCHSLQASGCGPDGRTCGYGYTYGDQSYTYGELGRDKITIGSFRLR
Sbjct: 126 RGSSTYRQVSCRSGTCHSLQASGCGPDGRTCGYGYTYGDQSYTYGELGRDKITIGSFRLR 185
Query: 181 NTVIGCGHENGGTFGSGASSGIIGLGGGSLSLVSQLNKIGAVRRRFSYCLPNIFRNANLT 240
NTVIGCGHENGGTFGSGASSGIIGLGGGSLSL SQLNKIGAVRRRFSYCLPNIFRNANLT
Sbjct: 186 NTVIGCGHENGGTFGSGASSGIIGLGGGSLSLASQLNKIGAVRRRFSYCLPNIFRNANLT 245
Query: 241 GTINFGGHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTRHAADMSSAVEGGMGNII 300
GTINFGGHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTRHAADMSSAVEGGMGNII
Sbjct: 246 GTINFGGHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTRHAADMSSAVEGGMGNII 305
Query: 301 IDSGTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYATNEIRDGHIPTITAHFG 360
IDSGTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYATNEIRDGHIPTITAHFG
Sbjct: 306 IDSGTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYATNEIRDGHIPTITAHFG 365
Query: 361 GCASVKLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLVGYDLHARRLSFKPTV 419
G A+VKLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLVGYDLHARRLSFKPTV
Sbjct: 366 GSAAVKLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLVGYDLHARRLSFKPTV 424
BLAST of MC10g0778 vs. NCBI nr
Match:
KAG5589183.1 (hypothetical protein H5410_039697 [Solanum commersonii])
HSP 1 Score: 679 bits (1752), Expect = 2.50e-230
Identity = 391/854 (45.78%), Postives = 527/854 (61.71%), Query Frame = 0
Query: 19 NGFTASLFHRDSSLSPY--------DRLNNAVRRSFSRSATLFRRAAAGVSTTAAKFPII 78
NGFT HRDS LSP+ +RL NA RSFSR A+ F++++ T + I
Sbjct: 35 NGFTLDFIHRDSPLSPFYNPSNTQSNRLRNAFHRSFSR-ASFFKKSSLATPNTI-QSDIS 94
Query: 79 PANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSGPIFNPRGSSTYRQVS 138
P GE+LM++S+GTPPV+ V IADTGSDLTWTQC+PC CF QS P+F+ + SSTY+ V
Sbjct: 95 PIPGEYLMKLSIGTPPVEIVAIADTGSDLTWTQCMPCENCFQQSSPLFDSKKSSTYKTVG 154
Query: 139 CRSGTCHSLQASGCGPDGRTCGYGYTYGDQSYTYGELGRDKITIGSFRLRNTVI-----G 198
C C SL+ S C G C Y +YGDQS+T G+L DK T S N VI G
Sbjct: 155 CNVEVCTSLEGSSC-VKGNVCEYQMSYGDQSHTIGDLAFDKFTFPSTSGENVVIPNVAFG 214
Query: 199 CGHENGGTFGSGASSGIIGLGGGSLSLVSQLNKIGAVRRRFSYCL-PNIFR---NANLTG 258
CGH+NGGTF + +SGIIGLGGG +S+++QL+K + +FSYCL P F N+N+T
Sbjct: 215 CGHDNGGTF-NNYTSGIIGLGGGKVSMINQLDK--EINGKFSYCLIPIPFDSSINSNITS 274
Query: 259 TINFGGHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGN-------TRHAADMSSAVEG 318
INFG A+VSG VSTPL+ K P TYYYL LE VSVGN ++ ++D + +G
Sbjct: 275 HINFGSSAIVSGPNVVSTPLIKKEPSTYYYLNLEGVSVGNKMLKFKSSKTSSDNAGGGDG 334
Query: 319 GMGNIIIDSGTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYATNEIRDGHI-- 378
GNIIIDSGTTLT LP + Y + STL IRA R +DP+G LCY E ++G I
Sbjct: 335 QAGNIIIDSGTTLTLLPNDFYSNLESTLVNSIRANRKDDPSGTFHLCY---ESQNGTIDA 394
Query: 379 PTITAHFGGCASVKLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLVGYDLHARR 438
PTI HF A ++L P +TF E+ + + CLT P+ E+AIFGNLAQ NFL+ YDL A +
Sbjct: 395 PTIVTHFTN-ADLELSPSSTFAEIEQGLVCLTIVPADEIAIFGNLAQGNFLIEYDLVANK 454
Query: 439 LSFKPTVSTTYGRGGFTTSLFHRDSLLSPLRRPSLSHYDRLHHAFRRSFARSAALFNRAS 498
+SF+PT L HRDS LSP PS + ++RL AF RSF+R++ +
Sbjct: 455 ISFQPT-----------DYLIHRDSPLSPYHDPSNTPFERLQKAFHRSFSRASFFGGK-- 514
Query: 499 AVASPAAIQSPLTPGSGEYLIEVSIGTPPVDFLAIADTGSDLVWTQCLPCRKCFNQSLPI 558
S +IQS L+P GE+L+++SIGTPP+D IADTGSDL WTQC PC CF Q PI
Sbjct: 515 ---SVPSIQSTLSPNEGEFLMKISIGTPPIDTFVIADTGSDLTWTQCEPCDNCFKQLTPI 574
Query: 559 FDPRRSSSFRHVACTSDPCRILDVSRCGAHQICDYSYTYGDQSYTKGELGFDKITLGS-- 618
F+P+ SSS++ + C + C+ SRC + C+Y YGD S+T+G+L + S
Sbjct: 575 FNPKNSSSYKTIGCHNKLCQ---RSRCN-NNTCNYLVIYGDLSHTRGDLSSETFRFASSS 634
Query: 619 ---FPLSKTVVGCGHESDGGFGDIASGVIGLGGGPLSLVSQLNKYSNVTRRFSYCL---P 678
+ V GCGH++ G F ++ SG+ GLGGG +S+V+Q+ + + +FSYCL
Sbjct: 635 GKNVSIPNIVFGCGHDNGGSFTNVTSGITGLGGGNVSIVNQM--HHEIKGKFSYCLIPLE 694
Query: 679 NVYSQAKLTAKINFGGNAVVSGSGVVSTPFVPKLPVTYYYITLEAVSVGNQRHE----AA 738
+ + T+ INFG NA+VSG G++STP T+YY+ L+ +S+GN+ E
Sbjct: 695 LLLDSSNATSHINFGDNAIVSGPGIISTPIFKNESPTFYYLDLKGISIGNKTLEFKSSPI 754
Query: 739 AKMSFAAGNMIIDSGTTLTFLPQELYDSVVSSLVRVVRARRVEDPGGVLGLCFAAEEGRG 798
++ GN+IIDSGTTLT++P Y ++ S L+R + A R +DP L LC+ + E G
Sbjct: 755 SQPGDDLGNIIIDSGTTLTYVPDGFYLNLESLLMRSINATRKDDPFKRLDLCYESNE-NG 814
Query: 799 GVDFPTITAHFSGGADVRLPAVNTFERVADDVSCLTMA--SSSDFGILGNLAQMNFLIGY 832
+D P I AHF+ AD+ L N F +V + + CLT+ S+ ILGNLAQ NFLIGY
Sbjct: 815 TIDVPKIVAHFTN-ADLELSTSNIFTQVVEGIVCLTIVPGGSNQISILGNLAQANFLIGY 854
BLAST of MC10g0778 vs. NCBI nr
Match:
KAA3468560.1 (aspartic proteinase CDR1-like [Gossypium australe])
HSP 1 Score: 660 bits (1703), Expect = 2.04e-223
Identity = 381/837 (45.52%), Postives = 521/837 (62.25%), Query Frame = 0
Query: 20 GFTASLFHRDSSLSPY--------DRLNNAVRRSFSRSATLFRRAAAGVSTTAAKFPIIP 79
GF+ LFHRDS SP+ DR+ NA+RRSF+R + R V TTAA+ +
Sbjct: 34 GFSVELFHRDSINSPFYNPLETTSDRVTNALRRSFNR---VHRFKTNSVPTTAAESDLTA 93
Query: 80 ANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSGPIFNPRGSSTYRQVSC 139
+GE+LM++SLGTP V IADTGSDL WTQC PC+QCF Q P F+P SSTYR++SC
Sbjct: 94 DSGEYLMKISLGTPRFDIVAIADTGSDLIWTQCKPCSQCFKQDAPFFDPSKSSTYRKISC 153
Query: 140 RSGTCHSLQASGCGPDGRTCGYGYTYGDQSYTYGELGRDKITIGSFRLR-----NTVIGC 199
+ C L+ + C D +C Y +YGD S++ G+L D +T+ S R TVIGC
Sbjct: 154 SASQCIDLERTSCSTD-HSCQYAVSYGDSSFSDGDLAADTLTLASITGRPVTFPKTVIGC 213
Query: 200 GHENGGTFGSGASSGIIGLGGGSLSLVSQLNKIGAVRRRFSYCLPNIFRNANLTGTINFG 259
G NGGTF +SGIIGLGGG +SL+SQL +V +FSYCL I + N + INFG
Sbjct: 214 GTSNGGTFDE-KTSGIIGLGGGQVSLISQLRT--SVAGKFSYCLLPISQAGN-SSKINFG 273
Query: 260 GHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTRHAADMSSAVEGGMGNIIIDSGTT 319
+A+VSG G VSTPLV K+PDT+Y+LTLEA++VG T+ S++ GNIIIDSGTT
Sbjct: 274 SNAIVSGPGVVSTPLVKKSPDTFYFLTLEAITVG-TKRIKFTGSSLGSEEGNIIIDSGTT 333
Query: 320 LTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCY-ATNEIRDGHIPTITAHFGGCASV 379
LT LP + Y V S +T I AKR E P G L LCY A +E + IP +T HF A +
Sbjct: 334 LTLLPSDFYSEVESAMTSQISAKRIEGPEG-LSLCYNAKDEFK---IPDVTVHFTN-ADM 393
Query: 380 KLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLVGYDLHARRLSFKPTVSTTYGR 439
KL PLNTF V++ C +F+ ++AI+GNL+QM+FL+GYD K TVS
Sbjct: 394 KLKPLNTFIRVSDTAICFSFSSLDDVAIYGNLSQMDFLIGYDTQ------KQTVSV---- 453
Query: 440 GGFTTSLFHRDSLLSPLRRPSLSHYDRLHHAFRRSFARSAALFNRA--SAVASPAAIQSP 499
L HRDS+ SP P + +DR+ +AFRRSF+R + + + A+P I +
Sbjct: 454 -----ELIHRDSIKSPFYNPFETTFDRVTNAFRRSFSRVHRFYPNSITTTEANPDIIVN- 513
Query: 500 LTPGSGEYLIEVSIGTPPVDFLAIADTGSDLVWTQCLPCRKCFNQSLPIFDPRRSSSFRH 559
+GEYL+ +S+GTP +A+ADTGSDL+WTQC PC +CF Q P+FDP +SS++R
Sbjct: 514 ----TGEYLMNISLGTPSFSVVALADTGSDLIWTQCSPCSQCFKQDAPLFDPTKSSTYRK 573
Query: 560 VACTSDPCRILDVSRCGA--HQICDYSYTYGDQSYTKGELGFDKITLGS-----FPLSKT 619
++C+S+ C + C + C YS TYGD S++KG++ +D +TLGS L T
Sbjct: 574 MSCSSNSCENIQGGTCASPTDTSCIYSVTYGDNSFSKGDIAYDTLTLGSTTGQAVALPDT 633
Query: 620 VVGCGHESDGGFGDIASGVIGLGGGPLSLVSQLNKYSNVTRRFSYCLPNVYSQAKLTAKI 679
++GCG+ + G F ASG+IGLGGG +SL++QL S + +FSYCL + +Q ++K+
Sbjct: 634 IIGCGNNNAGTFSGKASGIIGLGGGEISLINQLG--SPINGKFSYCLLPM-TQIGKSSKM 693
Query: 680 NFGGNAVVSGSGVVSTPFVPKLPVTYYYITLEAVSVGNQRHE-AAAKMSFAAGNMIIDSG 739
NFG NA+VSG G VSTP + K P T+Y++TL+A+SVG QR E + + GN++IDSG
Sbjct: 694 NFGSNAIVSGPGTVSTPLIEKSPNTFYFLTLKAISVGTQRIEFKGSSLGTDEGNIVIDSG 753
Query: 740 TTLTFLPQELYDSVVSSLVRVVRARRVEDPGGVLGLCFAAEEGRGGVDFPTITAHFSGGA 799
TTLT +P + Y + S++ R + P G LC+ A + P +T HF+ A
Sbjct: 754 TTLTLIPSDFYSQLESAMDSQFNGIRAQGPQG-FNLCYVAIHE---FEAPEVTVHFAN-A 813
Query: 800 DVRLPAVNTFERVADDVSCLTMASSSDFGILGNLAQMNFLIGYDMEAMRLSFKSTVC 832
DV+L +NTF +V D +C + + + I GNLAQMNFLIGYD ++ +SFK T C
Sbjct: 814 DVKLKTLNTFVKVDDTTACFAFSPAQNIAIYGNLAQMNFLIGYDTKSQAVSFKPTDC 828
BLAST of MC10g0778 vs. NCBI nr
Match:
RDY01103.1 (Aspartic proteinase CDR1, partial [Mucuna pruriens])
HSP 1 Score: 642 bits (1656), Expect = 5.89e-216
Identity = 373/847 (44.04%), Postives = 513/847 (60.57%), Query Frame = 0
Query: 20 GFTASLFHRDSSLSPY--------DRLNNAVRRSFSRSATLFRRAAAGVSTTAAKFPIIP 79
GF+ L HRDS SP+ +LNNA RSFSR + ++ A T + +I
Sbjct: 23 GFSVELIHRDSPKSPFYNPIETPFQQLNNAFHRSFSRVNHFYPKSKASQKTPQS---VIT 82
Query: 80 AN-GEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSGPIFNPRGSSTYRQVS 139
+N GE+L++ S+GTPP + +GIADTGSDL W+QC PC+QC+NQ+ P+F+P SSTY VS
Sbjct: 83 SNQGEYLVKYSIGTPPFEVMGIADTGSDLIWSQCKPCDQCYNQTTPLFDPSKSSTYEPVS 142
Query: 140 CRSGTCHSLQASGC--GPDGRTCGYGYTYGDQSYTYGELGRDKITI-----GSFRLRNTV 199
C S C L + C C Y +YGD S++ G L D T+ S
Sbjct: 143 CYSRVCQLLGKTYCYSANGDPNCEYTVSYGDGSHSQGTLAFDTFTLDSTTGSSVAFTKIS 202
Query: 200 IGCGHENGGTFGSGASSGIIGLGGGSLSLVSQLNKIGAVRRRFSYCLPNIFRNANLTGTI 259
IGCG N GTF S SGI+GLGGG +SL+SQ+ ++ +FSYCL +F + +++ +
Sbjct: 203 IGCGVNNAGTFDS-KGSGIVGLGGGVVSLISQIGP--SIDFKFSYCLVPLFESKSIS-KL 262
Query: 260 NFGGHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTRHAADMSSAVEGGMGNIIIDS 319
NFG +AVV+G G VSTP++P DT+YYL LE +SVG+ R S GNIIIDS
Sbjct: 263 NFGENAVVAGPGTVSTPIIPGPVDTFYYLKLEGMSVGSKRIEFICDSTSNVANGNIIIDS 322
Query: 320 GTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYATNEIRDGHIPTITAHFGGCA 379
GTTLT LP+ Y + + I +R IL LCY + P ITAHF G A
Sbjct: 323 GTTLTILPEKFYTKLELEVAAHINLERVNSTDQILSLCYQSPPNNAIETPIITAHFSG-A 382
Query: 380 SVKLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLVGYDLHARRLSFKPTVSTTY 439
V L LNTF V+ V+C FAP + +IFGNLAQMN+LVGYDL + +SFKPT T
Sbjct: 383 DVVLNSLNTFISVSNYVTCFAFAPMATNSIFGNLAQMNYLVGYDLQRKTVSFKPTDCTKI 442
Query: 440 GR-------GGFTTSLFHRDSLLSPLRRPSLSHYDRLHHAFRRSFARSAALFNRASAVAS 499
G+ GGF+ L HRDS SPL PS S + +L AF+RSF R + ++
Sbjct: 443 GKLESEALKGGFSVQLIHRDSSKSPLYNPSESAFQQLKSAFQRSFNRVNHFYPKSKVSRK 502
Query: 500 PAAIQSPLTPGSGEYLIEVSIGTPPVDFLAIADTGSDLVWTQCLPCRKCFNQSLPIFDPR 559
QS +T GEYL++ SIGTPP + + + DTGSDL+W+QC PC++C+NQ+ P+FD
Sbjct: 503 TKTPQSVITWNHGEYLVKYSIGTPPFEVMGVFDTGSDLIWSQCKPCKECYNQTNPLFDYS 562
Query: 560 RSSSFRHVACTSDPCRILDVSRCGAHQ--ICDYSYTYGDQSYTKGELGFDKITL-----G 619
+SS++ + C S C+ L + C +H C+Y+ YGD S+++G L FD +TL
Sbjct: 563 KSSTYEPIHCKSRVCKSLGEANCYSHSDPTCEYTVIYGDGSHSRGFLAFDTLTLPSTTDS 622
Query: 620 SFPLSKTVVGCGHESDGGFGDIASGVIGLGGGPLSLVSQLNKYSNVTRRFSYCLPNVYSQ 679
S K GCG + G F ASG++G+GGG +SL+SQ+ ++ +FSYCL ++S+
Sbjct: 623 SIAFPKIFFGCGVNNGGIFDPKASGIVGVGGGAVSLISQIGP--SIDFKFSYCLVPLFSE 682
Query: 680 AKLTAKINFGGNAVVSGSGVVSTPFVPKLPVTYYYITLEAVSVGNQRHE--AAAKMSFAA 739
++ T+K+NFG NAVV+G G VSTP +P T+YY+ L+ +SVG++R E + +K +
Sbjct: 683 SESTSKLNFGENAVVAGPGTVSTPIIPGPVNTFYYLKLKGMSVGSKRIELISDSKSNNGK 742
Query: 740 GNMIIDSGTTLTFLPQELYDSVVSSLVRVVRARRVEDPGGVLGLCFAAEEGRGGVDFPTI 799
GN+IIDSGTTLTFLPQ+LY + S + ++ RV P VL LC+ + ++ P I
Sbjct: 743 GNIIIDSGTTLTFLPQKLYTKLESEVAAQIKLERVHSPEHVLSLCYKSPSNNA-IEAPII 802
Query: 800 TAHFSGGADVRLPAVNTFERVADDVSCLTMAS--SSDFGILGNLAQMNFLIGYDMEAMRL 832
TAHFSG ADV L ++NTF V+D+V+C A SD I GN+AQMN L+GYD++ +
Sbjct: 803 TAHFSG-ADVVLNSLNTFVSVSDNVTCFAFAPVMRSD-SIFGNIAQMNHLVGYDLQKKTV 856
BLAST of MC10g0778 vs. NCBI nr
Match:
PHU08739.1 (Aspartic proteinase CDR1 [Capsicum chinense])
HSP 1 Score: 630 bits (1624), Expect = 2.28e-211
Identity = 376/845 (44.50%), Postives = 499/845 (59.05%), Query Frame = 0
Query: 20 GFTASLFHRDSSLSPYDRLNNAVRRSFSRS---ATLFRRAAAGVSTTAAKFPIIPANGEF 79
GFT L HRDS SP+ +N+VR +F RS A+ F++++ + + I P GE+
Sbjct: 40 GFTLDLIHRDSPQSPFYNPSNSVRDAFHRSFSRASFFQKSSFATPDSI-QSSISPIPGEY 99
Query: 80 LMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSGPIFNPRGSSTYRQVSCRSGTC 139
LM++S+GTPPV+ V IADTGSDLTWTQC PC CF QS P+F+ + SSTY+ + C + C
Sbjct: 100 LMKLSIGTPPVEIVAIADTGSDLTWTQCQPCINCFQQSYPLFDSKKSSTYKNIGCDAQEC 159
Query: 140 HSL-QASGCGPDGRTCGYGYTYGDQSYTYGELGRDKITIGSFRLRNTVI-----GCGHEN 199
S+ +S C C Y YGD S+T G+L D T S N I GCGH+N
Sbjct: 160 TSIGSSSSCVSGKNVCEYQINYGDNSHTIGDLAFDVFTFHSTSSENVAIPNVAFGCGHDN 219
Query: 200 GGTFGSGASSGIIGLGGGSLSLVSQLNKIGAVRRRFSYCLPNIFRNA----NLTGTINFG 259
GGTF + +SGIIGLGGG +S++ QL+K + +FSYCL I ++ N+T INFG
Sbjct: 220 GGTF-NNHTSGIIGLGGGEVSIIKQLDK--QINGKFSYCLIPIQLDSTSISNVTSHINFG 279
Query: 260 GHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTR---HAADMSSAVEGG-MGNIIID 319
+A+VSG VSTPL+ T+YYLTLE VSVGN ++ + + GG GNIIID
Sbjct: 280 SNAIVSGPDVVSTPLIRMESSTFYYLTLEGVSVGNKTLEFKSSKIGGSYAGGDEGNIIID 339
Query: 320 SGTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYATNEIRDGHIPTITAHFGGC 379
SGTTLT +P Y+ + S L I A R ED +G LCY ++E PTI AHF
Sbjct: 340 SGTTLTLMPSEFYENLESALVDSINATRKEDSSGTFGLCYDSDENGTIDAPTIVAHFTN- 399
Query: 380 ASVKLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLVGYDLHARRLSFKPTVSTT 439
A ++L P +TF +V + + CLT P+ E AIFGNLAQ NFL+G +
Sbjct: 400 ADLQLSPSSTFAQVEKGLVCLTIVPAKEFAIFGNLAQANFLIGVN--------------- 459
Query: 440 YGRGGFTTSLFHRDSLLSPLRRPSLSHYDRLHHAFRRSFARSAALFNRASAVASPAAIQS 499
GFT L HRDS LSP S + ++RL +AF+RSF+R+ + F S A IQS
Sbjct: 460 ----GFTLDLIHRDSPLSPFYNLSSTPFERLQNAFQRSFSRANSFFKGNSVNA----IQS 519
Query: 500 PLTPGSGEYLIEVSIGTPPVDFLAIADTGSDLVWTQCLPCRKCFNQSLPIFDPRRSSSFR 559
+TP GEYL+++SIGTPPV+ +AIADTGSDL WTQC PC CF QS P+FD +SS++
Sbjct: 520 IITPIPGEYLMKISIGTPPVEIVAIADTGSDLTWTQCKPCILCFQQSRPLFDSNKSSTYT 579
Query: 560 HVACTSDPCRIL-DVSRCGAHQICDYSYTYGDQSYTKGELGFDKITLGSFPLSKTVV--- 619
+C C + S CG IC+Y +Y QS T G+L FDK TL S V+
Sbjct: 580 TRSCKDKECTSIGSFSYCGDGNICEYRISYAGQSRTFGDLAFDKFTLPSTSGKNVVIPNV 639
Query: 620 --GCGHESDGGFGDIASGVIGLGGGPLSLVSQLNKYSNVTRRFSYCLP----NVYSQAKL 679
GCGHE+ G F SG+IGLGGG +S++ QL+K+ N +FSYCL S + +
Sbjct: 640 AFGCGHENSGTFSKYTSGIIGLGGGDVSIIKQLDKHIN--GKFSYCLIPFPLESTSMSNV 699
Query: 680 TAKINFGGNAVVSGSGVVSTPFVPKLPVTYYYITLEAVSVGNQRHE-----AAAKMSFAA 739
T+ INFG A++ G VVSTP + P T+YY+ L+ VSVGN + E +
Sbjct: 700 TSHINFGNRAMLPGPNVVSTPLIKNDPSTFYYLHLKGVSVGNNKLEFKFSKIGSYTGGYE 759
Query: 740 GNMIIDSGTTLTFLPQELYDSVVSSLVRVVRARRVEDPGGVLGLCFAAEEGRGGVDFPTI 799
GN+IIDSGTT T LP + + A R +DP G GL + +++G +D P I
Sbjct: 760 GNIIIDSGTTFTLLPMDS-----------INATRKDDPLGTYGLRYESKDGT--IDAPKI 819
Query: 800 TAHFSGGADVRLPAVNTFERVADDVSCLTMASSSDFGILGNLAQMNFLIGYDMEAMRLSF 832
+HF+ AD+ L NTF +V + CLT+A + D I GNLAQ NFLIGYD+ A ++SF
Sbjct: 820 VSHFTN-ADLELSPSNTFAQVQKGLVCLTIAPTDDIAIFGNLAQANFLIGYDLVANKVSF 840
BLAST of MC10g0778 vs. ExPASy TrEMBL
Match:
A0A6J1C4J6 (aspartic proteinase CDR1-like OS=Momordica charantia OX=3673 GN=LOC111008307 PE=3 SV=1)
HSP 1 Score: 833 bits (2152), Expect = 6.15e-298
Identity = 416/419 (99.28%), Postives = 417/419 (99.52%), Query Frame = 0
Query: 1 VFFILLFFSFSEATTHSTNGFTASLFHRDSSLSPYDRLNNAVRRSFSRSATLFRRAAAGV 60
VFFILLFFSFSEATTHSTNGFTASLFHRDSSLSPYDRLNNAVRRSFSRSATLFRRAAAGV
Sbjct: 6 VFFILLFFSFSEATTHSTNGFTASLFHRDSSLSPYDRLNNAVRRSFSRSATLFRRAAAGV 65
Query: 61 STTAAKFPIIPANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSGPIFNP 120
STTAAKFPIIPANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSGPIFNP
Sbjct: 66 STTAAKFPIIPANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSGPIFNP 125
Query: 121 RGSSTYRQVSCRSGTCHSLQASGCGPDGRTCGYGYTYGDQSYTYGELGRDKITIGSFRLR 180
RGSSTYRQVSCRSGTCHSLQASGCGPDGRTCGYGYTYGDQSYTYGELGRDKITIGSFRLR
Sbjct: 126 RGSSTYRQVSCRSGTCHSLQASGCGPDGRTCGYGYTYGDQSYTYGELGRDKITIGSFRLR 185
Query: 181 NTVIGCGHENGGTFGSGASSGIIGLGGGSLSLVSQLNKIGAVRRRFSYCLPNIFRNANLT 240
NTVIGCGHENGGTFGSGASSGIIGLGGGSLSL SQLNKIGAVRRRFSYCLPNIFRNANLT
Sbjct: 186 NTVIGCGHENGGTFGSGASSGIIGLGGGSLSLASQLNKIGAVRRRFSYCLPNIFRNANLT 245
Query: 241 GTINFGGHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTRHAADMSSAVEGGMGNII 300
GTINFGGHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTRHAADMSSAVEGGMGNII
Sbjct: 246 GTINFGGHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTRHAADMSSAVEGGMGNII 305
Query: 301 IDSGTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYATNEIRDGHIPTITAHFG 360
IDSGTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYATNEIRDGHIPTITAHFG
Sbjct: 306 IDSGTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYATNEIRDGHIPTITAHFG 365
Query: 361 GCASVKLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLVGYDLHARRLSFKPTV 419
G A+VKLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLVGYDLHARRLSFKPTV
Sbjct: 366 GSAAVKLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLVGYDLHARRLSFKPTV 424
BLAST of MC10g0778 vs. ExPASy TrEMBL
Match:
A0A3Q7HJU2 (Uncharacterized protein OS=Solanum lycopersicum OX=4081 PE=3 SV=1)
HSP 1 Score: 677 bits (1746), Expect = 1.49e-224
Identity = 390/850 (45.88%), Postives = 521/850 (61.29%), Query Frame = 0
Query: 19 NGFTASLFHRDSSLSPY--------DRLNNAVRRSFSRSATLFRRAAAGVSTTAAKFPII 78
NGFT L HRDS LSP +RL NA RSFSR++ F + ++ V+ + I
Sbjct: 34 NGFTLHLIHRDSPLSPLYNSSITQSNRLINAFHRSFSRAS--FFKKSSFVTPNTIRSDIS 93
Query: 79 PANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSGPIFNPRGSSTYRQVS 138
P GE++M++S+GTPPV+ V IADTGSDLTWTQC PC CF QS P+F+ + SS+Y+
Sbjct: 94 PIPGEYIMKLSIGTPPVEIVAIADTGSDLTWTQCEPCLNCFEQSSPLFDSKKSSSYKTAG 153
Query: 139 CRSGTCHSLQASGCGPDGRTCGYGYTYGDQSYTYGELGRDKITIGSFR------LRNTVI 198
C + C S+ +S C G C Y +YGDQSYT G+L D T S + N
Sbjct: 154 CDTKECTSIGSSSC-VKGNVCEYQMSYGDQSYTIGDLAFDIFTFPSTNSSENVAIPNVAF 213
Query: 199 GCGHENGGTFGSGASSGIIGLGGGSLSLVSQLNKIGAVRRRFSYCLPNIFRNA---NLTG 258
GCGH NGGTF + +SGIIGLGGG++S+++QL+K + +FSYCL +I + N+T
Sbjct: 214 GCGHHNGGTF-NNHTSGIIGLGGGNVSIINQLDK--EINGKFSYCLISIALGSPISNVTS 273
Query: 259 TINFGGHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTRHAADMSSAVEGGM-GNII 318
INFG A VSG VSTPL+ K P T+YYL LE VSVGN S GG GNII
Sbjct: 274 HINFGSSASVSGPDVVSTPLIKKEPSTFYYLNLEGVSVGNRTLKFKSSKVSSGGEEGNII 333
Query: 319 IDSGTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYATNEIRDGHI--PTITAH 378
IDSGTTLT LP Y + STL I A R EDP+G LCY E ++G I PTIT H
Sbjct: 334 IDSGTTLTLLPNEFYSSLESTLVDSISATRKEDPSGTFRLCY---ESKNGTIDAPTITTH 393
Query: 379 FGGCASVKLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLVGYDLHARRLSFKPT 438
F A ++L P +TF ++ E + CLT P+ E+AIFGNLAQ NFL+GYDL A ++SFKP
Sbjct: 394 FTN-ADLELSPSSTFAQIEEGLVCLTIVPADEIAIFGNLAQGNFLIGYDLVANKISFKPA 453
Query: 439 VSTTYGRGGFTTSLFHRDSLLSPLRRPSLSHYDRLHHAFRRSFARSAALFNRASAVASPA 498
T FT L HRDS LSP PS + Y+RL HA RSF+R++ L + +P
Sbjct: 454 DCTN-----FTLDLIHRDSPLSPFHNPSNTPYERLQHALYRSFSRASFLKKK---YVNP- 513
Query: 499 AIQSPLTPGSGEYLIEVSIGTPPVDFLAIADTGSDLVWTQCLPCRKCFNQSLPIFDPRRS 558
I+S L P GEYL+++SIGTPP+D L IADTGSDL WTQC PC CF Q PIF+P++S
Sbjct: 514 -IESTLIPSGGEYLMKISIGTPPIDTLVIADTGSDLTWTQCKPCVNCFKQLTPIFNPKKS 573
Query: 559 SSFRHVACTSDPCR--ILDVSRCGAHQICDYSYTYGDQSYTKGELGFDKITLGS-----F 618
SS++ + C + C+ + + SRC +Y +YGDQS+T G+L + T S
Sbjct: 574 SSYKTIGCNNKLCQGSLCNNSRC------NYEVSYGDQSHTMGDLSIETFTFSSTSSQNV 633
Query: 619 PLSKTVVGCGHESDGGFGDIASGVIGLGGGPLSLVSQLNKYSNVTRRFSYCL---PNVYS 678
+ V GCGH++ G F ++ SG+IGLGGG +S+V+Q+++ + +FSYCL ++
Sbjct: 634 SIPNIVFGCGHDNGGTFPNVTSGIIGLGGGNVSIVNQMHQ--QIKGKFSYCLIPLESLLD 693
Query: 679 QAKLTAKINFGGNAVVSGSGVVSTPFVPKLPVTYYYITLEAVSVGNQRHEAAAKMSFAAG 738
+ T+ INFG A VSG VVSTP + K P T+YY+ LE +S+GN+ E + G
Sbjct: 694 NSNATSHINFGNCATVSGPNVVSTPLIKKEPSTFYYLNLERISIGNRTVEFNSFPVVVGG 753
Query: 739 -----NMIIDSGTTLTFLPQELYDSVVSSLVRVVRARRVEDPGGVLGLCFAAEEGRGGVD 798
N+IIDSGTTLT++P Y ++ S L+ + A + +DP LC+ + + G +D
Sbjct: 754 DDDPGNIIIDSGTTLTYVPDAFYLNLESMLILSINATKKDDPSSSFRLCYESNK-NGTID 813
Query: 799 FPTITAHFSGGADVRLPAVNTFERVADDVSCLTMA-SSSDFGILGNLAQMNFLIGYDMEA 832
P I AHF+ AD+ L N F +V + + CLT+ + I GNLAQ NFLIGYD++A
Sbjct: 814 VPKIVAHFTN-ADLELSTSNIFTKVVEGIVCLTIVPGGNQISIFGNLAQANFLIGYDLKA 853
BLAST of MC10g0778 vs. ExPASy TrEMBL
Match:
A0A5B6VH54 (Aspartic proteinase CDR1-like OS=Gossypium australe OX=47621 GN=EPI10_014435 PE=3 SV=1)
HSP 1 Score: 660 bits (1703), Expect = 9.90e-224
Identity = 381/837 (45.52%), Postives = 521/837 (62.25%), Query Frame = 0
Query: 20 GFTASLFHRDSSLSPY--------DRLNNAVRRSFSRSATLFRRAAAGVSTTAAKFPIIP 79
GF+ LFHRDS SP+ DR+ NA+RRSF+R + R V TTAA+ +
Sbjct: 34 GFSVELFHRDSINSPFYNPLETTSDRVTNALRRSFNR---VHRFKTNSVPTTAAESDLTA 93
Query: 80 ANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSGPIFNPRGSSTYRQVSC 139
+GE+LM++SLGTP V IADTGSDL WTQC PC+QCF Q P F+P SSTYR++SC
Sbjct: 94 DSGEYLMKISLGTPRFDIVAIADTGSDLIWTQCKPCSQCFKQDAPFFDPSKSSTYRKISC 153
Query: 140 RSGTCHSLQASGCGPDGRTCGYGYTYGDQSYTYGELGRDKITIGSFRLR-----NTVIGC 199
+ C L+ + C D +C Y +YGD S++ G+L D +T+ S R TVIGC
Sbjct: 154 SASQCIDLERTSCSTD-HSCQYAVSYGDSSFSDGDLAADTLTLASITGRPVTFPKTVIGC 213
Query: 200 GHENGGTFGSGASSGIIGLGGGSLSLVSQLNKIGAVRRRFSYCLPNIFRNANLTGTINFG 259
G NGGTF +SGIIGLGGG +SL+SQL +V +FSYCL I + N + INFG
Sbjct: 214 GTSNGGTFDE-KTSGIIGLGGGQVSLISQLRT--SVAGKFSYCLLPISQAGN-SSKINFG 273
Query: 260 GHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTRHAADMSSAVEGGMGNIIIDSGTT 319
+A+VSG G VSTPLV K+PDT+Y+LTLEA++VG T+ S++ GNIIIDSGTT
Sbjct: 274 SNAIVSGPGVVSTPLVKKSPDTFYFLTLEAITVG-TKRIKFTGSSLGSEEGNIIIDSGTT 333
Query: 320 LTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCY-ATNEIRDGHIPTITAHFGGCASV 379
LT LP + Y V S +T I AKR E P G L LCY A +E + IP +T HF A +
Sbjct: 334 LTLLPSDFYSEVESAMTSQISAKRIEGPEG-LSLCYNAKDEFK---IPDVTVHFTN-ADM 393
Query: 380 KLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLVGYDLHARRLSFKPTVSTTYGR 439
KL PLNTF V++ C +F+ ++AI+GNL+QM+FL+GYD K TVS
Sbjct: 394 KLKPLNTFIRVSDTAICFSFSSLDDVAIYGNLSQMDFLIGYDTQ------KQTVSV---- 453
Query: 440 GGFTTSLFHRDSLLSPLRRPSLSHYDRLHHAFRRSFARSAALFNRA--SAVASPAAIQSP 499
L HRDS+ SP P + +DR+ +AFRRSF+R + + + A+P I +
Sbjct: 454 -----ELIHRDSIKSPFYNPFETTFDRVTNAFRRSFSRVHRFYPNSITTTEANPDIIVN- 513
Query: 500 LTPGSGEYLIEVSIGTPPVDFLAIADTGSDLVWTQCLPCRKCFNQSLPIFDPRRSSSFRH 559
+GEYL+ +S+GTP +A+ADTGSDL+WTQC PC +CF Q P+FDP +SS++R
Sbjct: 514 ----TGEYLMNISLGTPSFSVVALADTGSDLIWTQCSPCSQCFKQDAPLFDPTKSSTYRK 573
Query: 560 VACTSDPCRILDVSRCGA--HQICDYSYTYGDQSYTKGELGFDKITLGS-----FPLSKT 619
++C+S+ C + C + C YS TYGD S++KG++ +D +TLGS L T
Sbjct: 574 MSCSSNSCENIQGGTCASPTDTSCIYSVTYGDNSFSKGDIAYDTLTLGSTTGQAVALPDT 633
Query: 620 VVGCGHESDGGFGDIASGVIGLGGGPLSLVSQLNKYSNVTRRFSYCLPNVYSQAKLTAKI 679
++GCG+ + G F ASG+IGLGGG +SL++QL S + +FSYCL + +Q ++K+
Sbjct: 634 IIGCGNNNAGTFSGKASGIIGLGGGEISLINQLG--SPINGKFSYCLLPM-TQIGKSSKM 693
Query: 680 NFGGNAVVSGSGVVSTPFVPKLPVTYYYITLEAVSVGNQRHE-AAAKMSFAAGNMIIDSG 739
NFG NA+VSG G VSTP + K P T+Y++TL+A+SVG QR E + + GN++IDSG
Sbjct: 694 NFGSNAIVSGPGTVSTPLIEKSPNTFYFLTLKAISVGTQRIEFKGSSLGTDEGNIVIDSG 753
Query: 740 TTLTFLPQELYDSVVSSLVRVVRARRVEDPGGVLGLCFAAEEGRGGVDFPTITAHFSGGA 799
TTLT +P + Y + S++ R + P G LC+ A + P +T HF+ A
Sbjct: 754 TTLTLIPSDFYSQLESAMDSQFNGIRAQGPQG-FNLCYVAIHE---FEAPEVTVHFAN-A 813
Query: 800 DVRLPAVNTFERVADDVSCLTMASSSDFGILGNLAQMNFLIGYDMEAMRLSFKSTVC 832
DV+L +NTF +V D +C + + + I GNLAQMNFLIGYD ++ +SFK T C
Sbjct: 814 DVKLKTLNTFVKVDDTTACFAFSPAQNIAIYGNLAQMNFLIGYDTKSQAVSFKPTDC 828
BLAST of MC10g0778 vs. ExPASy TrEMBL
Match:
A0A371HE86 (Aspartic proteinase CDR1 (Fragment) OS=Mucuna pruriens OX=157652 GN=CDR1 PE=3 SV=1)
HSP 1 Score: 642 bits (1656), Expect = 2.85e-216
Identity = 373/847 (44.04%), Postives = 513/847 (60.57%), Query Frame = 0
Query: 20 GFTASLFHRDSSLSPY--------DRLNNAVRRSFSRSATLFRRAAAGVSTTAAKFPIIP 79
GF+ L HRDS SP+ +LNNA RSFSR + ++ A T + +I
Sbjct: 23 GFSVELIHRDSPKSPFYNPIETPFQQLNNAFHRSFSRVNHFYPKSKASQKTPQS---VIT 82
Query: 80 AN-GEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSGPIFNPRGSSTYRQVS 139
+N GE+L++ S+GTPP + +GIADTGSDL W+QC PC+QC+NQ+ P+F+P SSTY VS
Sbjct: 83 SNQGEYLVKYSIGTPPFEVMGIADTGSDLIWSQCKPCDQCYNQTTPLFDPSKSSTYEPVS 142
Query: 140 CRSGTCHSLQASGC--GPDGRTCGYGYTYGDQSYTYGELGRDKITI-----GSFRLRNTV 199
C S C L + C C Y +YGD S++ G L D T+ S
Sbjct: 143 CYSRVCQLLGKTYCYSANGDPNCEYTVSYGDGSHSQGTLAFDTFTLDSTTGSSVAFTKIS 202
Query: 200 IGCGHENGGTFGSGASSGIIGLGGGSLSLVSQLNKIGAVRRRFSYCLPNIFRNANLTGTI 259
IGCG N GTF S SGI+GLGGG +SL+SQ+ ++ +FSYCL +F + +++ +
Sbjct: 203 IGCGVNNAGTFDS-KGSGIVGLGGGVVSLISQIGP--SIDFKFSYCLVPLFESKSIS-KL 262
Query: 260 NFGGHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTRHAADMSSAVEGGMGNIIIDS 319
NFG +AVV+G G VSTP++P DT+YYL LE +SVG+ R S GNIIIDS
Sbjct: 263 NFGENAVVAGPGTVSTPIIPGPVDTFYYLKLEGMSVGSKRIEFICDSTSNVANGNIIIDS 322
Query: 320 GTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYATNEIRDGHIPTITAHFGGCA 379
GTTLT LP+ Y + + I +R IL LCY + P ITAHF G A
Sbjct: 323 GTTLTILPEKFYTKLELEVAAHINLERVNSTDQILSLCYQSPPNNAIETPIITAHFSG-A 382
Query: 380 SVKLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLVGYDLHARRLSFKPTVSTTY 439
V L LNTF V+ V+C FAP + +IFGNLAQMN+LVGYDL + +SFKPT T
Sbjct: 383 DVVLNSLNTFISVSNYVTCFAFAPMATNSIFGNLAQMNYLVGYDLQRKTVSFKPTDCTKI 442
Query: 440 GR-------GGFTTSLFHRDSLLSPLRRPSLSHYDRLHHAFRRSFARSAALFNRASAVAS 499
G+ GGF+ L HRDS SPL PS S + +L AF+RSF R + ++
Sbjct: 443 GKLESEALKGGFSVQLIHRDSSKSPLYNPSESAFQQLKSAFQRSFNRVNHFYPKSKVSRK 502
Query: 500 PAAIQSPLTPGSGEYLIEVSIGTPPVDFLAIADTGSDLVWTQCLPCRKCFNQSLPIFDPR 559
QS +T GEYL++ SIGTPP + + + DTGSDL+W+QC PC++C+NQ+ P+FD
Sbjct: 503 TKTPQSVITWNHGEYLVKYSIGTPPFEVMGVFDTGSDLIWSQCKPCKECYNQTNPLFDYS 562
Query: 560 RSSSFRHVACTSDPCRILDVSRCGAHQ--ICDYSYTYGDQSYTKGELGFDKITL-----G 619
+SS++ + C S C+ L + C +H C+Y+ YGD S+++G L FD +TL
Sbjct: 563 KSSTYEPIHCKSRVCKSLGEANCYSHSDPTCEYTVIYGDGSHSRGFLAFDTLTLPSTTDS 622
Query: 620 SFPLSKTVVGCGHESDGGFGDIASGVIGLGGGPLSLVSQLNKYSNVTRRFSYCLPNVYSQ 679
S K GCG + G F ASG++G+GGG +SL+SQ+ ++ +FSYCL ++S+
Sbjct: 623 SIAFPKIFFGCGVNNGGIFDPKASGIVGVGGGAVSLISQIGP--SIDFKFSYCLVPLFSE 682
Query: 680 AKLTAKINFGGNAVVSGSGVVSTPFVPKLPVTYYYITLEAVSVGNQRHE--AAAKMSFAA 739
++ T+K+NFG NAVV+G G VSTP +P T+YY+ L+ +SVG++R E + +K +
Sbjct: 683 SESTSKLNFGENAVVAGPGTVSTPIIPGPVNTFYYLKLKGMSVGSKRIELISDSKSNNGK 742
Query: 740 GNMIIDSGTTLTFLPQELYDSVVSSLVRVVRARRVEDPGGVLGLCFAAEEGRGGVDFPTI 799
GN+IIDSGTTLTFLPQ+LY + S + ++ RV P VL LC+ + ++ P I
Sbjct: 743 GNIIIDSGTTLTFLPQKLYTKLESEVAAQIKLERVHSPEHVLSLCYKSPSNNA-IEAPII 802
Query: 800 TAHFSGGADVRLPAVNTFERVADDVSCLTMAS--SSDFGILGNLAQMNFLIGYDMEAMRL 832
TAHFSG ADV L ++NTF V+D+V+C A SD I GN+AQMN L+GYD++ +
Sbjct: 803 TAHFSG-ADVVLNSLNTFVSVSDNVTCFAFAPVMRSD-SIFGNIAQMNHLVGYDLQKKTV 856
BLAST of MC10g0778 vs. ExPASy TrEMBL
Match:
F6HJ51 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_02s0087g00230 PE=3 SV=1)
HSP 1 Score: 638 bits (1646), Expect = 1.37e-214
Identity = 378/868 (43.55%), Postives = 508/868 (58.53%), Query Frame = 0
Query: 8 FSFSEATTHSTNGFTASLFHRDSSLSPY--------DRLNNAVRRSFSRSATLFRRAAAG 67
F E GF+ L HRDS SP+ +RL +A RRS SR FR A
Sbjct: 19 FQLLEVALARGGGFSVDLIHRDSPHSPFFDPSKTQAERLTDAFRRSVSRVGR-FRPTA-- 78
Query: 68 VSTTAAKFPIIPANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSGPIFN 127
+++ + I+P+ GE+LM + +GTPPV + I DTGSDLTWTQC PC C+ Q P+F+
Sbjct: 79 MTSDGIQSRIVPSAGEYLMNLYIGTPPVPVIAIVDTGSDLTWTQCRPCTHCYKQVVPLFD 138
Query: 128 PRGSSTYRQVSCRSGTCHSLQASGCGPDGRTCGYGYTYGDQSYTYGELGRDKITIGS--- 187
P+ SSTYR SC + C +L + C + Y+Y D S+T G L + +T+ S
Sbjct: 139 PKNSSTYRDSSCGTSFCLALGKDRSCSKEKKCTFRYSYADGSFTGGNLASETLTVDSTAG 198
Query: 188 --FRLRNTVIGCGHENGGTFGSGASSGIIGLGGGSLSLVSQLNKIGAVRRRFSYCLPNIF 247
GCGH +GG F +SSGI+GLGGG LSL+SQL + FSYCL +
Sbjct: 199 KPVSFPGFAFGCGHSSGGIFDK-SSSGIVGLGGGELSLISQLKS--TINGLFSYCLLPVS 258
Query: 248 RNANLTGTINFGGHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTRHA-ADMSSAVE 307
+++++ INFG VSG G VSTPLV K+PDT+YYLTLE +SVG R S E
Sbjct: 259 TDSSISSRINFGASGRVSGYGTVSTPLVQKSPDTFYYLTLEGISVGKKRLPYKGYSKKTE 318
Query: 308 GGMGNIIIDSGTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYATNEIRDGHIP 367
GNII+DSGTT TFLPQ Y + ++ I+ KR DP GI LCY N + + P
Sbjct: 319 VEEGNIIVDSGTTYTFLPQEFYSKLEKSVANSIKGKRVRDPNGIFSLCY--NTTAEINAP 378
Query: 368 TITAHFGGCASVKLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLVGYDLHARRL 427
ITAHF A+V+L PLNTF + E++ C T AP+S++ + GNLAQ+NFLVG+DL +R+
Sbjct: 379 IITAHFKD-ANVELQPLNTFMRMQEDLVCFTVAPTSDIGVLGNLAQVNFLVGFDLRKKRI 438
Query: 428 S---------FKPTVSTTY----------GRGGFTTSLFHRDSLLSPLRRPSLSHYDRLH 487
S F V + GGF+ L HRDS SP PS + +RL
Sbjct: 439 SSMEVFGVKIFFNVVVVGFLFHLLEVGLASGGGFSVDLIHRDSPHSPFFDPSKTRTERLT 498
Query: 488 HAFRRSFARSAALFNRASAVASPAAIQSPLTPGSGEYLIEVSIGTPPVDFLAIADTGSDL 547
AF RS +R R SA+ S IQS L P +GEY++ +SIGTPPV +AI DTGSDL
Sbjct: 499 DAFHRSASRVGRF--RQSAMTSDG-IQSRLVPSAGEYIMNLSIGTPPVPVIAIVDTGSDL 558
Query: 548 VWTQCLPCRKCFNQSLPIFDPRRSSSFRHVACTSDPCRILDVSR-CGAHQICDYSYTYGD 607
WTQC PC C+ Q +P FDP+ SS++R +C + C L R C + C + Y+Y D
Sbjct: 559 TWTQCRPCTHCYKQVVPFFDPKNSSTYRDSSCGTSFCLALGNDRSCRNGKKCTFMYSYAD 618
Query: 608 QSYTKGELGFDKITLGSF---PLSKT--VVGCGHESDGGFGDIASGVIGLGGGPLSLVSQ 667
S+T G L + +T+ S P+S GC H S G F + +SG++GLG LS++SQ
Sbjct: 619 GSFTGGNLAVETLTVASTAGKPVSFPGFAFGCVHRSGGIFDEHSSGIVGLGVAELSMISQ 678
Query: 668 LNKYSNVTRRFSYCLPNVYSQAKLTAKINFGGNAVVSGSGVVSTPFVPKLPVTYYY-ITL 727
L S + RFSYCL V++ + ++++INFG + +VSG+G VSTP V K P TYYY ITL
Sbjct: 679 LK--STINGRFSYCLLPVFTDSSMSSRINFGRSGIVSGAGTVSTPLVMKGPDTYYYLITL 738
Query: 728 EAVSVGNQRHEA---AAKMSFAAGNMIIDSGTTLTFLPQELYDSVVSSLVRVVRARRVED 787
E SVG +R + K GN+I+DSGTT T+LP E Y + S+ ++ +RV D
Sbjct: 739 EGFSVGKKRLSYKGFSKKAEVEEGNIIVDSGTTYTYLPLEFYVKLEESVAHSIKGKRVRD 798
Query: 788 PGGVLGLCFAAEEGRGGVDFPTITAHFSGGADVRLPAVNTFERVADDVSCLTMASSSDFG 832
P G+ LC+ + +D P ITAHF A+V L NTF R+ +D+ C T+ +SD G
Sbjct: 799 PNGISSLCYNTTVDQ--IDAPIITAHFKD-ANVELQPWNTFLRMQEDLVCFTVLPTSDIG 858
BLAST of MC10g0778 vs. TAIR 10
Match:
AT2G28220.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 388.7 bits (997), Expect = 1.2e-107
Identity = 289/860 (33.60%), Postives = 413/860 (48.02%), Query Frame = 0
Query: 1 VFFILLFFSFSEATT-HSTNGFTASLFHRDSSLSPYDRLNNAVRRSFSRSATLFRRAAAG 60
V F+ + F TT S +GFT L R S+ S + N ++ + + TLF
Sbjct: 24 VLFLQIITCFLFTTTVSSPHGFTIDLIQRRSNSSSFRLSKNQLQGASPYADTLF------ 83
Query: 61 VSTTAAKFPIIPANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSGPIFN 120
+LM++ +GTPP + DTGSDL WTQC+PC C++Q PIF+
Sbjct: 84 ------------DYNIYLMKLQVGTPPFEIAAEIDTGSDLIWTQCMPCPDCYSQFDPIFD 143
Query: 121 PRGSSTYRQVSCRSGTCHSLQASGCGPDGRTCGYGYTYGDQSYTYGELGRDKITIGS--- 180
P SST+ + C G++C Y Y D +Y+ G L + +TI S
Sbjct: 144 PSKSSTFNEQRCH---------------GKSCHYEIIYEDNTYSKGILATETVTIHSTSG 203
Query: 181 --FRLRNTVIGCGHENGGTFGSG---ASSGIIGLGGGSLSLVSQLNKIGAVRRRFSYCLP 240
F + T IGCG N SG +SSGI+GL G SL+SQ++ SYC
Sbjct: 204 EPFVMAETTIGCGLHNTDLDNSGFASSSSGIVGLNMGPRSLISQMDL--PYPGLISYCF- 263
Query: 241 NIFRNANLTGTINFGGHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTRHAADMSSA 300
+ T INFG +A+V+G G V+ + K + +YYL L+AVSV + R + +
Sbjct: 264 ----SGQGTSKINFGTNAIVAGDGTVAADMFIKKDNPFYYLNLDAVSVEDNR-IETLGTP 323
Query: 301 VEGGMGNIIIDSGTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYATNEIRDGH 360
GNI+IDSG+T+T+ P + + V + +V+ A R DP+G LCY + I
Sbjct: 324 FHAEDGNIVIDSGSTVTYFPVSYCNLVRKAVEQVVTAVRVPDPSGNDMLCYFSETI--DI 383
Query: 361 IPTITAHFGGCASVKLLPLNTFGEV-AENVSCLTF---APSSELAIFGNLAQMNFLVGYD 420
P IT HF G A + L N + E + + CL +P+ E AIFGN AQ NFLVGYD
Sbjct: 384 FPVITMHFSGGADLVLDKYNMYMESNSGGLFCLAIICNSPTQE-AIFGNRAQNNFLVGYD 443
Query: 421 LHARRLSFKPTVSTTYGRGGFTTSLFHRDSLLSPLRRPSLSHYDRLHHAFRRSFARSAAL 480
S+ L
Sbjct: 444 -------------------------------------------------------SSSLL 503
Query: 481 FNRASAVASPAAIQSPLTPGSGEYLIEVSIGTPPVDFLAIADTGSDLVWTQCLPCRKCFN 540
AS A S YL+++ +GTPP + +A DTGSD++WTQC+PC C++
Sbjct: 504 LQGASPYADTLYDYS-------IYLMKLQVGTPPFEIVAEIDTGSDIIWTQCMPCPNCYS 563
Query: 541 QSLPIFDPRRSSSFRHVACTSDPCRILDVSRCGAHQICDYSYTYGDQSYTKGELGFDKIT 600
Q PIFDP +SS+FR C + C Y Y D++Y+KG L + +T
Sbjct: 564 QFAPIFDPSKSSTFREQRCNGNSCH--------------YEIIYADKTYSKGILATETVT 623
Query: 601 LGS-----FPLSKTVVGCGHESD----GGFGDIASGVIGLGGGPLSLVSQLN-KYSNVTR 660
+ S F +++T +GCG ++ GF +SG++GL GPLSL+SQ++ Y +
Sbjct: 624 IPSTSGEPFVMAETKIGCGLDNTNLQYSGFASSSSGIVGLNMGPLSLISQMDLPYPGL-- 683
Query: 661 RFSYCLPNVYSQAKLTAKINFGGNAVVSGSGVVSTPFVPKLPVTYYYITLEAVSV-GNQR 720
SYC + T+KINFG NA+V+G G V+ K +YY+ L+AVSV N
Sbjct: 684 -ISYCF-----SGQGTSKINFGTNAIVAGDGTVAADMFIKKDNPFYYLNLDAVSVEDNLI 743
Query: 721 HEAAAKMSFAAGNMIIDSGTTLTFLPQELYDSVVSSLVRVVRARRVEDPGGVLGLCFAAE 780
GN+ IDSGTTLT+ P + V ++ +VV A +V D G LC+ ++
Sbjct: 744 ATLGTPFHAEDGNIFIDSGTTLTYFPMSYCNLVREAVEQVVTAVKVPDMGSDNLLCYYSD 751
Query: 781 EGRGGVD-FPTITAHFSGGADVRLPAVNTF-ERVADDVSCLTMASS--SDFGILGNLAQM 833
+D FP IT HFSGGAD+ L N + E + + CL + + S + GN AQ
Sbjct: 804 T----IDIFPVITMHFSGGADLVLDKYNMYLETITGGIFCLAIGCNDPSMPAVFGNRAQN 751
BLAST of MC10g0778 vs. TAIR 10
Match:
AT1G64830.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 374.0 bits (959), Expect = 3.1e-103
Identity = 213/429 (49.65%), Postives = 267/429 (62.24%), Query Frame = 0
Query: 4 ILLFFSFSEATTHSTNGFTASLFHRDSSLSPY--------DRLNNAVRRSFSRSATLFRR 63
+L S + +GFT L HRDS SP+ R+ NA+RRS +RS F
Sbjct: 9 LLSLLLLSNVNAYPKDGFTIDLIHRDSPKSPFYNSAETSSQRMRNAIRRS-ARSTLQFSN 68
Query: 64 AAAGVSTTAAKFPIIPANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQSG 123
A S + + I GE+LM +S+GTPPV + IADTGSDL WTQC PC C+ Q+
Sbjct: 69 DDA--SPNSPQSFITSNRGEYLMNISIGTPPVPILAIADTGSDLIWTQCNPCEDCYQQTS 128
Query: 124 PIFNPRGSSTYRQVSCRSGTCHSLQASGCGPDGRTCGYGYTYGDQSYTYGELGRDKITIG 183
P+F+P+ SSTYR+VSC S C +L+ + C D TC Y TYGD SYT G++ D +T+G
Sbjct: 129 PLFDPKESSTYRKVSCSSSQCRALEDASCSTDENTCSYTITYGDNSYTKGDVAVDTVTMG 188
Query: 184 S-----FRLRNTVIGCGHENGGTFGSGASSGIIGLGGGSLSLVSQLNKIGAVRRRFSYCL 243
S LRN +IGCGHEN GTF A SGIIGLGGGS SLVSQL K ++ +FSYCL
Sbjct: 189 SSGRRPVSLRNMIIGCGHENTGTF-DPAGSGIIGLGGGSTSLVSQLRK--SINGKFSYCL 248
Query: 244 PNIFRNANLTGTINFGGHAVVSGRGAVSTPLVPKNPDTYYYLTLEAVSVGNTRHAADMSS 303
LT INFG + +VSG G VST +V K+P TYY+L LEA+SVG+ + +S
Sbjct: 249 VPFTSETGLTSKINFGTNGIVSGDGVVSTSMVKKDPATYYFLNLEAISVGSKK--IQFTS 308
Query: 304 AVEG-GMGNIIIDSGTTLTFLPQNLYDGVVSTLTKVIRAKRAEDPAGILELCYATNEIRD 363
+ G G GNI+IDSGTTLT LP N Y + S + I+A+R +DP GIL LCY +
Sbjct: 309 TIFGTGEGNIVIDSGTTLTLLPSNFYYELESVVASTIKAERVQDPDGILSLCY--RDSSS 368
Query: 364 GHIPTITAHFGGCASVKLLPLNTFGEVAENVSCLTFAPSSELAIFGNLAQMNFLVGYDLH 419
+P IT HF G VKL LNTF V+E+VSC FA + +L IFGNLAQMNFLVGYD
Sbjct: 369 FKVPDITVHFKG-GDVKLGNLNTFVAVSEDVSCFAFAANEQLTIFGNLAQMNFLVGYDTV 426
BLAST of MC10g0778 vs. TAIR 10
Match:
AT5G33340.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 343.2 bits (879), Expect = 5.9e-94
Identity = 189/415 (45.54%), Postives = 258/415 (62.17%), Query Frame = 0
Query: 427 GFTTSLFHRDSLLSPLRRPSLSHYDRLHHAFRRSFARSAALFNRASAVASPAAIQSPLTP 486
GFT L HRDS SP P + RL +A RS R +F+ +P Q LT
Sbjct: 30 GFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNR---VFHFTEKDNTPQP-QIDLTS 89
Query: 487 GSGEYLIEVSIGTPPVDFLAIADTGSDLVWTQCLPCRKCFNQSLPIFDPRRSSSFRHVAC 546
SGEYL+ VSIGTPP +AIADTGSDL+WTQC PC C+ Q P+FDP+ SS+++ V+C
Sbjct: 90 NSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKTSSTYKDVSC 149
Query: 547 TSDPCRILD-VSRCGAH-QICDYSYTYGDQSYTKGELGFDKITLGS-----FPLSKTVVG 606
+S C L+ + C + C YS +YGD SYTKG + D +TLGS L ++G
Sbjct: 150 SSSQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSSDTRPMQLKNIIIG 209
Query: 607 CGHESDGGFGDIASGVIGLGGGPLSLVSQLNKYSNVTRRFSYCLPNVYSQAKLTAKINFG 666
CGH + G F SG++GLGGGP+SL+ QL ++ +FSYCL + S+ T+KINFG
Sbjct: 210 CGHNNAGTFNKKGSGIVGLGGGPVSLIKQLG--DSIDGKFSYCLVPLTSKKDQTSKINFG 269
Query: 667 GNAVVSGSGVVSTPFVPKL-PVTYYYITLEAVSVGNQRHEAAAKMSFAA-GNMIIDSGTT 726
NA+VSGSGVVSTP + K T+YY+TL+++SVG+++ + + S ++ GN+IIDSGTT
Sbjct: 270 TNAIVSGSGVVSTPLIAKASQETFYYLTLKSISVGSKQIQYSGSDSESSEGNIIIDSGTT 329
Query: 727 LTFLPQELYDSVVSSLVRVVRARRVEDPGGVLGLCFAAEEGRGGVDFPTITAHFSGGADV 786
LT LP E Y + ++ + A + +DP L LC++A G + P IT HF GADV
Sbjct: 330 LTLLPTEFYSELEDAVASSIDAEKKQDPQSGLSLCYSA---TGDLKVPVITMHFD-GADV 389
Query: 787 RLPAVNTFERVADDVSCLTMASSSDFGILGNLAQMNFLIGYDMEAMRLSFKSTVC 833
+L + N F +V++D+ C S F I GN+AQMNFL+GYD + +SFK T C
Sbjct: 390 KLDSSNAFVQVSEDLVCFAFRGSPSFSIYGNVAQMNFLVGYDTVSKTVSFKPTDC 434
BLAST of MC10g0778 vs. TAIR 10
Match:
AT2G35615.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 332.0 bits (850), Expect = 1.4e-90
Identity = 187/442 (42.31%), Postives = 273/442 (61.76%), Query Frame = 0
Query: 413 LSFKPTVSTTYGRGGFTTSLFHRDSLLSPLRRPSLSHYDRLHHAFRRSFARSAALFNRAS 472
L F T+S++ F+ L HRDS LSP+ P ++ DRL+ AF RS +RS R +
Sbjct: 11 LFFSVTLSSSGHPKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSRS----RRFN 70
Query: 473 AVASPAAIQSPLTPGSGEYLIEVSIGTPPVDFLAIADTGSDLVWTQCLPCRKCFNQSLPI 532
S +QS L GE+ + ++IGTPP+ AIADTGSDL W QC PC++C+ ++ PI
Sbjct: 71 HQLSQTDLQSGLIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKPCQQCYKENGPI 130
Query: 533 FDPRRSSSFRHVACTSDPCRILDVSRCG---AHQICDYSYTYGDQSYTKGELGFDKITLG 592
FD ++SS+++ C S C+ L + G ++ IC Y Y+YGDQS++KG++ + +++
Sbjct: 131 FDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQSFSKGDVATETVSID 190
Query: 593 S---FPLS--KTVVGCGHESDGGFGDIASGVIGLGGGPLSLVSQLNKYSNVTRRFSYCLP 652
S P+S TV GCG+ + G F + SG+IGLGGG LSL+SQL S+++++FSYCL
Sbjct: 191 SASGSPVSFPGTVFGCGYNNGGTFDETGSGIIGLGGGHLSLISQLG--SSISKKFSYCLS 250
Query: 653 NVYSQAKLTAKINFGGNAVVSG----SGVVSTPFVPKLPVTYYYITLEAVSVGNQRHEAA 712
+ + T+ IN G N++ S SGVVSTP V K P+TYYY+TLEA+SVG ++
Sbjct: 251 HKSATTNGTSVINLGTNSIPSSLSKDSGVVSTPLVDKEPLTYYYLTLEAISVGKKKIPYT 310
Query: 713 AK---------MSFAAGNMIIDSGTTLTFLPQELYDSVVSSLVR-VVRARRVEDPGGVLG 772
+S +GN+IIDSGTTLT L +D S++ V A+RV DP G+L
Sbjct: 311 GSSYNPNDDGILSETSGNIIIDSGTTLTLLEAGFFDKFSSAVEESVTGAKRVSDPQGLLS 370
Query: 773 LCFAAEEGRGGVDFPTITAHFSGGADVRLPAVNTFERVADDVSCLTMASSSDFGILGNLA 832
CF + G + P IT HF+ GADVRL +N F ++++D+ CL+M +++ I GN A
Sbjct: 371 HCF--KSGSAEIGLPEITVHFT-GADVRLSPINAFVKLSEDMVCLSMVPTTEVAIYGNFA 430
BLAST of MC10g0778 vs. TAIR 10
Match:
AT1G31450.1 (Eukaryotic aspartyl protease family protein )
HSP 1 Score: 312.4 bits (799), Expect = 1.1e-84
Identity = 190/439 (43.28%), Postives = 251/439 (57.18%), Query Frame = 0
Query: 5 LLFFSFSEATTHSTN--GFTASLFHRDSSLSPY--------DRLNNAVRRSFSRSATLFR 64
LL SF A+ S N T L HRDS SP DRLN A RS SRS
Sbjct: 11 LLAISFFFASNSSANRENLTVELIHRDSPHSPLYNPHHTVSDRLNAAFLRSISRSRRF-- 70
Query: 65 RAAAGVSTTAAKFPIIPANGEFLMEVSLGTPPVKFVGIADTGSDLTWTQCLPCNQCFNQS 124
+ T + +I GE+ M +S+GTPP K IADTGSDLTW QC PC QC+ Q+
Sbjct: 71 -----TTKTDLQSGLISNGGEYFMSISIGTPPSKVFAIADTGSDLTWVQCKPCQQCYKQN 130
Query: 125 GPIFNPRGSSTYRQVSCRSGTCHSL--QASGCGPDGRTCGYGYTYGDQSYTYGELGRDKI 184
P+F+ + SSTY+ SC S TC +L GC C Y Y+YGD S+T G++ + I
Sbjct: 131 SPLFDKKKSSTYKTESCDSKTCQALSEHEEGCDESKDICKYRYSYGDNSFTKGDVATETI 190
Query: 185 TI-----GSFRLRNTVIGCGHENGGTFGSGASSGIIGLGGGSLSLVSQLNKIGAVRRRFS 244
+I S TV GCG+ NGGTF SGIIGLGGG LSLVSQL ++ ++FS
Sbjct: 191 SIDSSSGSSVSFPGTVFGCGYNNGGTF-EETGSGIIGLGGGPLSLVSQLG--SSIGKKFS 250
Query: 245 YCLPNIFRNANLTGTINFGGHAVVSG----RGAVSTPLVPKNPDTYYYLTLEAVSVGNTR 304
YCL + N T IN G +++ S ++TPL+ K+P+TYY+LTLEAV+VG T+
Sbjct: 251 YCLSHTAATTNGTSVINLGTNSIPSNPSKDSATLTTPLIQKDPETYYFLTLEAVTVGKTK 310
Query: 305 -----HAADMSSAVEGGMGNIIIDSGTTLTFLPQNLYDGVVSTLTK-VIRAKRAEDPAGI 364
++ GNIIIDSGTTLT L YD + + + V AKR DP G+
Sbjct: 311 LPYTGGGYGLNGKSSKRTGNIIIDSGTTLTLLDSGFYDDFGTAVEESVTGAKRVSDPQGL 370
Query: 365 LELCYATNEIRDGHIPTITAHFGGCASVKLLPLNTFGEVAENVSCLTFAPSSELAIFGNL 417
L C+ + + G +P IT HF A VKL P+N F ++ E+ CL+ P++E+AI+GN+
Sbjct: 371 LTHCFKSGDKEIG-LPAITMHFTN-ADVKLSPINAFVKLNEDTVCLSMIPTTEVAIYGNM 430
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q6XBF8 | 8.2e-93 | 45.54 | Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1 | [more] |
Q3EBM5 | 1.9e-89 | 42.31 | Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g3561... | [more] |
Q766C3 | 1.4e-63 | 37.29 | Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... | [more] |
Q766C2 | 7.0e-60 | 36.13 | Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... | [more] |
Q9LNJ3 | 7.8e-51 | 32.79 | Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... | [more] |
Match Name | E-value | Identity | Description | |
XP_022136655.1 | 1.27e-297 | 99.28 | aspartic proteinase CDR1-like [Momordica charantia] | [more] |
KAG5589183.1 | 2.50e-230 | 45.78 | hypothetical protein H5410_039697 [Solanum commersonii] | [more] |
KAA3468560.1 | 2.04e-223 | 45.52 | aspartic proteinase CDR1-like [Gossypium australe] | [more] |
RDY01103.1 | 5.89e-216 | 44.04 | Aspartic proteinase CDR1, partial [Mucuna pruriens] | [more] |
PHU08739.1 | 2.28e-211 | 44.50 | Aspartic proteinase CDR1 [Capsicum chinense] | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1C4J6 | 6.15e-298 | 99.28 | aspartic proteinase CDR1-like OS=Momordica charantia OX=3673 GN=LOC111008307 PE=... | [more] |
A0A3Q7HJU2 | 1.49e-224 | 45.88 | Uncharacterized protein OS=Solanum lycopersicum OX=4081 PE=3 SV=1 | [more] |
A0A5B6VH54 | 9.90e-224 | 45.52 | Aspartic proteinase CDR1-like OS=Gossypium australe OX=47621 GN=EPI10_014435 PE=... | [more] |
A0A371HE86 | 2.85e-216 | 44.04 | Aspartic proteinase CDR1 (Fragment) OS=Mucuna pruriens OX=157652 GN=CDR1 PE=3 SV... | [more] |
F6HJ51 | 1.37e-214 | 43.55 | Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_02s0087g00230 PE=3 SV=... | [more] |