MC10g0745 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g0745
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	F-box/LRR-repeat protein 14 isoform X1
Location	MC10: 6225656 .. 6230969 (+)
RNA-Seq Expression	MC10g0745
Synteny	MC10g0745

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGAAATTAACATAAAATAGAAATTGAGGGCTGTGATGACTAAAAATGTAATTTACTAACAAGTTCATCGTTCCTTCCATGGATAAAAGTAAAACAGAAAAGAGAGCCGGTTCATCGTCCGCCAAATCTAGCGATTGGGAACCAAGAACAAGAACAAGTTTCTCTGATTGACAGGAGAGTTGAAATCCAGGAAACATTCTAAGAGATGGAGAATAAACTGGTGGAATTCTGCATCAATTCGGCCTGTGAGAGCAAGGACAGCGTCGAGAAGTGGCGGAGGCAGAAGCGAAGCCTTGAGCGTTTGCCCTCTCAACTCGCCGATGCTCTCCTTCGCCGTCTTCACGCTCGTCGTCTCCTCTATCCTTCTATTCTCGAGTACGTTTCAACTCCATTTTTGTTTCTGACTATCGAAGTAAGTTTGTTGAACGATCGAAAGCGGATTCAGAATTTTTAGGCTCTCATTTTGTCCATTGGAGACCGAGGTCTCTCATCAAGATGCAATACTTCTATATTGGATCGAAATGCCTGAAATTTGTATGAATTCGGATTTGATTAAGTACGTGGAGTGATCCTGCTGCTTGATGCTTCGTAATAGAATCGTAGCTTAGTTGAGATAAAATTAAGTTTCTCTTGATGGAGATGCTGGTAATCGTCCTCAATCTGGTTTTTACTGGAAACCTTTTAGAAAGTAGCTGAAGAGGTAGCTACAGCTTATACTTTTGGCATTTTTGCCTCCATTATTATGTATCAAAACATCATTCTTCTGTTTTATTAGCTGATATACATCAGGGAGTGCCAACAAAGTTTATGTCCTTTCTTTCTTTCTCACTGTTTTTCAACTATACTTGAGTAGGTTTAGGAAGTTATCCTTGTTGTTCACTAACGTTCTGCTGAACTCAGATGTTTTGATGCAGAGTTTTCAAATTTACCGTAGAGGTGGTTGATCTAAGTGGTGAAAATGCTGTTGATTCAGAATGGATGGCCTACTTAGGAGCATATCGTTACTTACGATCATTAAATCTTTCCAACTGCCACAGGCTTTCTAGTTCTGGTATATGGGCTATTGCAGGTATGTTATAGAAAATGATACAAATGTTAGTTTGAAGGCTGACTTGTAAGTCACAGAAAGTTCTGTGTCGAAGTGACTTAACCCTGTTGTGATTTCTCCACTAGTCCAGTGCCGTGGAAATCTTGCGTGTAGTTTCCACGAAAAGAAATATATTTTTTACTTTCTGTCCTCCTCTTATCAACTGTGGACATCGACAGGCCATCTAAATTTCAGTGGCTGCAATTTAGTTAAGTTTGATAATTATGATGTCCTATGCTTATTTATAGGGATGACTACTTTAAGGGAGTTGAACCTTTCGAGATGTTCGAAAGTTACTGATGCTGGAATTAGGCATCTTTTATCCATACCAACTTTGGAAAAGCTATGCATTGCTGAGACTGGCATCACTGCACATGGAGTTACACTCCTTTCTTCCTTAAAAACATTGGTGTTCTTGGATTTGGGTGGTCTAGCTGTCACCGACCAGGCCCTGAGTTCTCTTCAGGTTTTTCCTGTTTCCCTTTTTGGATTAACTGTGAATCCCATGTTCCTTTGTGGGACTGTGGCTGCATGGTATACACCTATTGTATCTTGCAGTTTCAACGCGTAGGCGTTTGCTTCCCTGCACTTGCACATGTCTACACCATCAAATAATGCTGGATTGATGGTTTGTCAGTCTTATTTGATAAGATGCGAACCTTTACAGATAATGTATTGCACGGCTATTGGATCTGTGAATGGCATAGGTTACTCAAGATGATTGTTTCTGTTGATGAAGTTTTGTCCCAATTGATGATTGGATTGCATTATAGCTTCTACAACAATCTAGTATATAGATGATGATATTGTTATTTCTCCACTCAAACTCATTCTAACTATTTACTTGTTCCAAGTAAATTCTGTCTATTTATTGTTTAATCTATTAAATTTTATTGCTCCTTCCTTGCAATTCATATTTTTGAATTAATATTTTTAATTTAATCCCATTTTCGTCCTACTCTCTTTTAGGTGCTCACAAAATTACAATACCTTGACCTTTGGGGTAGTAAAATATCTAACACTGGATCTGATGTTCTACAAATGTTTCCGAAGTTGAACTTCCTGAATATAGCTTGGACTAATGTCACCAAATTCCCAAACTTGCCTCATCTTGAGTCCCTGAACGTGAGTAACTGCGTAATTGACTCTACTATTAAAGGGTCTGGTGCTAAAGTTCCTCTAAGAAAGCTTATTGCCTCTGGAGCTACATTTTCAAACGAGACTGAAGACCTGGAATTTGTTGGAACCAGTTTCCTAAATCATCTGGACTTCTCGAATGCTTGTCTTCACAGATTCTGCTTCTTACCTCATATGAAAGCATTGGAACATTTGGACCTTAGCTCTACTGCCATAGGAGACAATTCGGCTGGATTAATTGCATCCGTTGGAGAAAATTTGAAACATCTAAACCTCAGCTGCACAACAGTCAGCTCTTCTGGAGTTGGGATTTTAGCTGGAAAAGTTCCAAACCTTGAAACTTTGTCACTATCCCACACCATGATTGATGATTTTGCCATATCATATATAAGTTTGATGCCTGGCTTGAAGAGCATTGACCTCAGCGAAACAGACATTAAAGGTATTGCTTTTGTACTAAAAAATGCTATGATGTACTTAATCAGGTCACGTATGCTGCATATTCCCCTTGCTCATTCTCTAGGGCGCTGTACACACACCAACTCCATCAGCTTCTTATCTAGAACCTTCTTTCAGTCCTTCTATCAGCTTCTTATCTAGCACCTTTCTTTCAGTCATTGTGCTGAACGCTTTTATGCTTCTGCATAGGAAGTTATTGTTTCCTTCTTAGTGCCAGGGCACAATATCCTTGTGCAGGTTTTATGTGAGTGATTTCACATGCCGGTGCATGTAATTCTATTGCCATTTCATTAACTGAATACTATTAAAAATATATATATCCTTTATTCTGTGTTATTTGATAGGAACAAAACAAAATTTATTCGAAACCATGGGCACCTATTGTTGCTGCTATTTTCACCATGGCTCTTTAAATCTGAGAAGTTTGCAGTCTTCTGATATTTTTTTGTCCTAATTTCAGGTTACATTTACCTATCAGCTGCGGAGGGAAATGAGTATCTCTCATTTACAGACTTGCAAAACCTTGATTGTTTAGAAATATTGAATTTGGAGCGCACTCATGTGGACGATGAATCTCTACGCCCATTATCAAGATTCCGAAGGCTGAGTCACTTGCTGCTTAGAAGTCCCTCATTGACAGATGTGTCACTGAGCTACTTGTCGATTTTGCCCAAGCTGACAATCCTCAGTATTCGGGATGCAGTTTTGACAAACCAGGCGTTTGATTTGCTTAAGCCTGCGGCAACTCTACAAAAGATTGACTTGAGAGGTTGTTGGCTGTTAACTGAAGATGGTCTTTTAGAATTTCACCGAAGGTTTCCACAAATTGAAGTTAGACATGAACTTTTTCACTTCTCATCTGATCAAACTTCCTCTGATCGACCTTCAACTCATTTTACTCCAAAGAAATTACAATTGAACCAGAGGAACAAATCTGCCGCCATGTCACCATATTTTGTAGGTATGTAACTCAGACAAATAATTCATATAAGATTTTAAGTCAAACTAAAACATAAATAGACACTTGGGATTAGTTCTTATCTTATATATAACAACTAAGAATCACATTAAGAATCACGGACACTTCAATTTGGTGTCGGTGTCGGATACTTTGATACTTATTGGACACTTGTTAGCTCAATAGATGTGTACAACACATTAGTCGTAGAAAGTGAATATAGATTCGACATTTATTAGGCACGTATCGAATACTTGTTAAGTGTAAGAAATAGACCTAAATAGTAACGTAGGATAAAATAATAAATTGAGAATAAAATACTAACTCATTTTTTAAAGTATAAAATTGCATAAACTTATTGACTTTGAATTTTCTTTCAGAATTAAAATGATATATTTTAATAACGTGTTTTTGTTGTGTTCGTGTCCCAAATTTTTAGAAAATGATGTGTCACCATGTCTGTGTCGTATCCATATCTCGTGTCCATATCCATGCTTCTTAGATATCACATATTCCTATATACGAAATAAAATCTGCTCTATGTAATTGACAAAACAGATATAAATATATCTTCCTTATGATGTAAAGTTTGATGTTCTGGTTAATATTGATTTTGGGCATTCAAATCAATGCAACCAATGTCAGTATTTAATCCTTGACTCTAAGTAGGTTATTGTTTACAAGGCTTATATGATTTCTTTCTTCCTTTCTTACCCTTATGCAATAGATCAAAGGTTGAAGTATAGTAGCGAAGAGCTACTTGCCCTACAGTTTTCTTCTCTGTCCCATGGTTCCACATCTACTCGTGAAATTGGTCAGAAATCTGATACCTGAAGATGCAGTAACACAAAGGTGCTTGAACTATTCTGAGAATTGAATTGTTTGGCAATCCTCTTTTTCTGTTGATGGCTCAGATTCATTATATTTAGATCTTTGAGTTCATTTACTAATTACGCGATCTATATTGGAAATGTAGTGAAGTTTTCGTTCAATGCACGGTTCAAATCACTTGCTTCTGGTTGAGATCAGAACTTGAAAGATAAGGGCCGAAACGTTCGAGATAGCATAGCAGGAGAAGGGAAGGACATTGAGCAAGGGGAGAAATCTTTCAATAGAGGAAAGGGATGAGAGGTTTAAATTTAAACAGAAGTTCTTTCAAATTTAATGGCAGGAGTGAAGTAGAGTTTTGCAGTGGAGTAAACTTGCATGGATTTTAAAAGAGGAGGATGAATAAATTAATCACTGTTGTTCCATAGATTGGTAGTTAGAAGGTGGAGTAAGTTAGAAGACGAAGGTATTTAAGAGGAAATCTTGAAGTTATTCCGTAATCGTAGCAGTTTTTCAGGAAGCTACAAATTGGCAGCCTCGAAGGTATTCTGAGAATTGAATTTTTTGGTAATCATATTTTATTATTGATGACTGAGGTTCATTGTATTTACATCGTTAAGTTTACTTACCAATTATGTGTTATGTATTGAAAATATAGTGAAGTTTTCTTCGAAACATGCAATAGCTTTGATTTTCTTTTTTTTCTTTTTTTCCATATTTATTTAAAGATTGCTAGTCATTTCAGAGAGAGATGATTCGTGATTTTCCGTTGAAGAAGAAAACGGAAAAGAAGAAAGTCAAAGCCACGAATGCCACCTCACTTGAAATAAATACATCAATAAATG

mRNA sequence

CGGAAATTAACATAAAATAGAAATTGAGGGCTGTGATGACTAAAAATGTAATTTACTAACAAGTTCATCGTTCCTTCCATGGATAAAAGTAAAACAGAAAAGAGAGCCGGTTCATCGTCCGCCAAATCTAGCGATTGGGAACCAAGAACAAGAACAAGTTTCTCTGATTGACAGGAGAGTTGAAATCCAGGAAACATTCTAAGAGATGGAGAATAAACTGGTGGAATTCTGCATCAATTCGGCCTGTGAGAGCAAGGACAGCGTCGAGAAGTGGCGGAGGCAGAAGCGAAGCCTTGAGCGTTTGCCCTCTCAACTCGCCGATGCTCTCCTTCGCCGTCTTCACGCTCGTCGTCTCCTCTATCCTTCTATTCTCGAAGTTTTCAAATTTACCGTAGAGGTGGTTGATCTAAGTGGTGAAAATGCTGTTGATTCAGAATGGATGGCCTACTTAGGAGCATATCGTTACTTACGATCATTAAATCTTTCCAACTGCCACAGGCTTTCTAGTTCTGGTATATGGGCTATTGCAGGGATGACTACTTTAAGGGAGTTGAACCTTTCGAGATGTTCGAAAGTTACTGATGCTGGAATTAGGCATCTTTTATCCATACCAACTTTGGAAAAGCTATGCATTGCTGAGACTGGCATCACTGCACATGGAGTTACACTCCTTTCTTCCTTAAAAACATTGGTGTTCTTGGATTTGGGTGGTCTAGCTGTCACCGACCAGGCCCTGAGTTCTCTTCAGGTGCTCACAAAATTACAATACCTTGACCTTTGGGGTAGTAAAATATCTAACACTGGATCTGATGTTCTACAAATGTTTCCGAAGTTGAACTTCCTGAATATAGCTTGGACTAATGTCACCAAATTCCCAAACTTGCCTCATCTTGAGTCCCTGAACGTGAGTAACTGCGTAATTGACTCTACTATTAAAGGGTCTGGTGCTAAAGTTCCTCTAAGAAAGCTTATTGCCTCTGGAGCTACATTTTCAAACGAGACTGAAGACCTGGAATTTGTTGGAACCAGTTTCCTAAATCATCTGGACTTCTCGAATGCTTGTCTTCACAGATTCTGCTTCTTACCTCATATGAAAGCATTGGAACATTTGGACCTTAGCTCTACTGCCATAGGAGACAATTCGGCTGGATTAATTGCATCCGTTGGAGAAAATTTGAAACATCTAAACCTCAGCTGCACAACAGTCAGCTCTTCTGGAGTTGGGATTTTAGCTGGAAAAGTTCCAAACCTTGAAACTTTGTCACTATCCCACACCATGATTGATGATTTTGCCATATCATATATAAGTTTGATGCCTGGCTTGAAGAGCATTGACCTCAGCGAAACAGACATTAAAGGTTACATTTACCTATCAGCTGCGGAGGGAAATGAGTATCTCTCATTTACAGACTTGCAAAACCTTGATTGTTTAGAAATATTGAATTTGGAGCGCACTCATGTGGACGATGAATCTCTACGCCCATTATCAAGATTCCGAAGGCTGAGTCACTTGCTGCTTAGAAGTCCCTCATTGACAGATGTGTCACTGAGCTACTTGTCGATTTTGCCCAAGCTGACAATCCTCAGTATTCGGGATGCAGTTTTGACAAACCAGGCGTTTGATTTGCTTAAGCCTGCGGCAACTCTACAAAAGATTGACTTGAGAGGTTGTTGGCTGTTAACTGAAGATGGTCTTTTAGAATTTCACCGAAGGTTTCCACAAATTGAAGTTAGACATGAACTTTTTCACTTCTCATCTGATCAAACTTCCTCTGATCGACCTTCAACTCATTTTACTCCAAAGAAATTACAATTGAACCAGAGGAACAAATCTGCCGCCATGTCACCATATTTTGTAGATCAAAGGTTGAAGTATAGTAGCGAAGAGCTACTTGCCCTACAGTTTTCTTCTCTGTCCCATGGTTCCACATCTACTCGTGAAATTGGTCAGAAATCTGATACCTGAAGATGCAGTAACACAAAGTGAAGTTTTCGTTCAATGCACGGTTCAAATCACTTGCTTCTGGTTGAGATCAGAACTTGAAAGATAAGGGCCGAAACGTTCGAGATAGCATAGCAGGAGAAGGGAAGGACATTGAGCAAGGGGAGAAATCTTTCAATAGAGGAAAGGGATGAGAGGTTTAAATTTAAACAGAAGTTCTTTCAAATTTAATGGCAGGAGTGAAGTAGAGTTTTGCAGTGGAGTAAACTTGCATGGATTTTAAAAGAGGAGGATGAATAAATTAATCACTGTTGTTCCATAGATTGGTAGTTAGAAGGTGGAGTAAGTTAGAAGACGAAGGTATTTAAGAGGAAATCTTGAAGTTATTCCGTAATCGTAGCAGTTTTTCAGGAAGCTACAAATTGGCAGCCTCGAAGGTATTCTGAGAATTGAATTTTTTGGTAATCATATTTTATTATTGATGACTGAGGTTCATTGTATTTACATCGTTAAGTTTACTTACCAATTATGTGTTATGTATTGAAAATATAGTGAAGTTTTCTTCGAAACATGCAATAGCTTTGATTTTCTTTTTTTTCTTTTTTTCCATATTTATTTAAAGATTGCTAGTCATTTCAGAGAGAGATGATTCGTGATTTTCCGTTGAAGAAGAAAACGGAAAAGAAGAAAGTCAAAGCCACGAATGCCACCTCACTTGAAATAAATACATCAATAAATG

Coding sequence (CDS)

ATGGAGAATAAACTGGTGGAATTCTGCATCAATTCGGCCTGTGAGAGCAAGGACAGCGTCGAGAAGTGGCGGAGGCAGAAGCGAAGCCTTGAGCGTTTGCCCTCTCAACTCGCCGATGCTCTCCTTCGCCGTCTTCACGCTCGTCGTCTCCTCTATCCTTCTATTCTCGAAGTTTTCAAATTTACCGTAGAGGTGGTTGATCTAAGTGGTGAAAATGCTGTTGATTCAGAATGGATGGCCTACTTAGGAGCATATCGTTACTTACGATCATTAAATCTTTCCAACTGCCACAGGCTTTCTAGTTCTGGTATATGGGCTATTGCAGGGATGACTACTTTAAGGGAGTTGAACCTTTCGAGATGTTCGAAAGTTACTGATGCTGGAATTAGGCATCTTTTATCCATACCAACTTTGGAAAAGCTATGCATTGCTGAGACTGGCATCACTGCACATGGAGTTACACTCCTTTCTTCCTTAAAAACATTGGTGTTCTTGGATTTGGGTGGTCTAGCTGTCACCGACCAGGCCCTGAGTTCTCTTCAGGTGCTCACAAAATTACAATACCTTGACCTTTGGGGTAGTAAAATATCTAACACTGGATCTGATGTTCTACAAATGTTTCCGAAGTTGAACTTCCTGAATATAGCTTGGACTAATGTCACCAAATTCCCAAACTTGCCTCATCTTGAGTCCCTGAACGTGAGTAACTGCGTAATTGACTCTACTATTAAAGGGTCTGGTGCTAAAGTTCCTCTAAGAAAGCTTATTGCCTCTGGAGCTACATTTTCAAACGAGACTGAAGACCTGGAATTTGTTGGAACCAGTTTCCTAAATCATCTGGACTTCTCGAATGCTTGTCTTCACAGATTCTGCTTCTTACCTCATATGAAAGCATTGGAACATTTGGACCTTAGCTCTACTGCCATAGGAGACAATTCGGCTGGATTAATTGCATCCGTTGGAGAAAATTTGAAACATCTAAACCTCAGCTGCACAACAGTCAGCTCTTCTGGAGTTGGGATTTTAGCTGGAAAAGTTCCAAACCTTGAAACTTTGTCACTATCCCACACCATGATTGATGATTTTGCCATATCATATATAAGTTTGATGCCTGGCTTGAAGAGCATTGACCTCAGCGAAACAGACATTAAAGGTTACATTTACCTATCAGCTGCGGAGGGAAATGAGTATCTCTCATTTACAGACTTGCAAAACCTTGATTGTTTAGAAATATTGAATTTGGAGCGCACTCATGTGGACGATGAATCTCTACGCCCATTATCAAGATTCCGAAGGCTGAGTCACTTGCTGCTTAGAAGTCCCTCATTGACAGATGTGTCACTGAGCTACTTGTCGATTTTGCCCAAGCTGACAATCCTCAGTATTCGGGATGCAGTTTTGACAAACCAGGCGTTTGATTTGCTTAAGCCTGCGGCAACTCTACAAAAGATTGACTTGAGAGGTTGTTGGCTGTTAACTGAAGATGGTCTTTTAGAATTTCACCGAAGGTTTCCACAAATTGAAGTTAGACATGAACTTTTTCACTTCTCATCTGATCAAACTTCCTCTGATCGACCTTCAACTCATTTTACTCCAAAGAAATTACAATTGAACCAGAGGAACAAATCTGCCGCCATGTCACCATATTTTGTAGATCAAAGGTTGAAGTATAGTAGCGAAGAGCTACTTGCCCTACAGTTTTCTTCTCTGTCCCATGGTTCCACATCTACTCGTGAAATTGGTCAGAAATCTGATACCTGA

Protein sequence

MENKLVEFCINSACESKDSVEKWRRQKRSLERLPSQLADALLRRLHARRLLYPSILEVFKFTVEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSRCSKVTDAGIRHLLSIPTLEKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSLQVLTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVIDSTIKGSGAKVPLRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALEHLDLSSTAIGDNSAGLIASVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMIDDFAISYISLMPGLKSIDLSETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVDDESLRPLSRFRRLSHLLLRSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAATLQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQRNKSAAMSPYFVDQRLKYSSEELLALQFSSLSHGSTSTREIGQKSDT

Homology

[Close]

BLAST of MC10g0745 vs. ExPASy Swiss-Prot
Match: Q17R01 (F-box/LRR-repeat protein 14 OS=Bos taurus OX=9913 GN=FBXL14 PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.2e-05
Identity = 44/165 (26.67%), Postives = 86/165 (52.12%), Query Frame = 0


Query: 63  VEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIA-GMTTLRELNLSRC 122
           + +++LS    +    + +L     LRSLNL +C  +S +GI  +A G   L  L++S C
Sbjct: 230 LRLLNLSFCGGISDAGLLHLSHMGSLRSLNLRSCDNISDTGIMHLAMGSLRLSGLDVSFC 289

Query: 123 SKVTDAGIRHLL-SIPTLEKLCIAETGITAHGVT-LLSSLKTLVFLDLGG-LAVTDQALS 182
            KV D  + ++   +  L+ L +    I+  G+  ++  +  L  L++G  + +TD+ L 
Sbjct: 290 DKVGDQSLAYIAQGLDGLKSLSLCSCHISDDGINRMVRQMHGLRTLNIGQCVRITDKGLE 349

Query: 183 SL-QVLTKLQYLDLWG-SKISNTGSDVLQMFPKLNFLNIAWTNVT 222
            + + L++L  +DL+G ++I+  G + +   P L  LN+    +T
Sbjct: 350 LIAEHLSQLTGIDLYGCTRITKRGLERITQLPCLKVLNLGLWQMT 394

[Close]

BLAST of MC10g0745 vs. ExPASy Swiss-Prot
Match: Q8N1E6 (F-box/LRR-repeat protein 14 OS=Homo sapiens OX=9606 GN=FBXL14 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.2e-05
Identity = 44/165 (26.67%), Postives = 86/165 (52.12%), Query Frame = 0


Query: 63  VEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIA-GMTTLRELNLSRC 122
           + +++LS    +    + +L     LRSLNL +C  +S +GI  +A G   L  L++S C
Sbjct: 230 LRLLNLSFCGGISDAGLLHLSHMGSLRSLNLRSCDNISDTGIMHLAMGSLRLSGLDVSFC 289

Query: 123 SKVTDAGIRHLL-SIPTLEKLCIAETGITAHGVT-LLSSLKTLVFLDLGG-LAVTDQALS 182
            KV D  + ++   +  L+ L +    I+  G+  ++  +  L  L++G  + +TD+ L 
Sbjct: 290 DKVGDQSLAYIAQGLDGLKSLSLCSCHISDDGINRMVRQMHGLRTLNIGQCVRITDKGLE 349

Query: 183 SL-QVLTKLQYLDLWG-SKISNTGSDVLQMFPKLNFLNIAWTNVT 222
            + + L++L  +DL+G ++I+  G + +   P L  LN+    +T
Sbjct: 350 LIAEHLSQLTGIDLYGCTRITKRGLERITQLPCLKVLNLGLWQMT 394

[Close]

BLAST of MC10g0745 vs. ExPASy Swiss-Prot
Match: Q8BID8 (F-box/LRR-repeat protein 14 OS=Mus musculus OX=10090 GN=Fbxl14 PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.2e-05
Identity = 44/165 (26.67%), Postives = 86/165 (52.12%), Query Frame = 0


Query: 63  VEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIA-GMTTLRELNLSRC 122
           + +++LS    +    + +L     LRSLNL +C  +S +GI  +A G   L  L++S C
Sbjct: 230 LRLLNLSFCGGISDAGLLHLSHMGSLRSLNLRSCDNISDTGIMHLAMGSLRLSGLDVSFC 289

Query: 123 SKVTDAGIRHLL-SIPTLEKLCIAETGITAHGVT-LLSSLKTLVFLDLGG-LAVTDQALS 182
            KV D  + ++   +  L+ L +    I+  G+  ++  +  L  L++G  + +TD+ L 
Sbjct: 290 DKVGDQSLAYIAQGLDGLKSLSLCSCHISDDGINRMVRQMHGLRTLNIGQCVRITDKGLE 349

Query: 183 SL-QVLTKLQYLDLWG-SKISNTGSDVLQMFPKLNFLNIAWTNVT 222
            + + L++L  +DL+G ++I+  G + +   P L  LN+    +T
Sbjct: 350 LIAEHLSQLTGIDLYGCTRITKRGLERITQLPCLKVLNLGLWQMT 394

[Close]

BLAST of MC10g0745 vs. ExPASy Swiss-Prot
Match: Q8NEE6 (Dynein regulatory complex subunit 6 OS=Homo sapiens OX=9606 GN=FBXL13 PE=2 SV=3)

HSP 1 Score: 51.2 bits (121), Expect = 4.6e-05
Identity = 54/198 (27.27%), Postives = 97/198 (48.99%), Query Frame = 0


Query: 61  FTVEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRE-LNLS 120
           F++  +DLSG + + +E +  L  ++ L+ L++S C+R++  GI A    + + E L++S
Sbjct: 530 FSLVSIDLSGTD-ISNEGLNVLSRHKKLKELSVSECYRITDDGIQAFCKSSLILEHLDVS 589

Query: 121 RCSKVTDAGIRHL-LSIPTLEKLCIAE-TGITAHGVTLLSS-LKTLVFLDLGG-LAVTDQ 180
            CS+++D  I+ L +    L  L IA    IT   + +LS+    L  LD+ G + +TDQ
Sbjct: 590 YCSQLSDMIIKALAIYCINLTSLSIAGCPKITDSAMEMLSAKCHYLHILDISGCVLLTDQ 649

Query: 181 ALSSLQV------LTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPN-LPH 240
            L  LQ+      + K+QY      K +   S  +Q           W    +  N +  
Sbjct: 650 ILEDLQIGCKQLRILKMQYCTNISKKAAQRMSSKVQQQEYNTNDPPRWFGYDREGNPVTE 709

Query: 241 LESLNVSNCVIDSTIKGS 247
           L+++  S   ++ T+K S
Sbjct: 710 LDNITSSKGALELTVKKS 726

[Close]

BLAST of MC10g0745 vs. ExPASy Swiss-Prot
Match: Q8CDU4 (Dynein regulatory complex subunit 6 OS=Mus musculus OX=10090 GN=Fbxl13 PE=1 SV=2)

HSP 1 Score: 48.5 bits (114), Expect = 3.0e-04
Identity = 45/175 (25.71%), Postives = 81/175 (46.29%), Query Frame = 0


Query: 85  YRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSRCSKVTDAGIRHLLSIPT---LEKL 144
           Y  +  + + +C  L+ S + +++ +  L  LNL+ C ++ D G++H    P    L +L
Sbjct: 476 YPGINHIYMVDCKGLTDSSLKSLSLLKQLTVLNLTNCIRIGDIGLKHFFDGPASIRLREL 535

Query: 145 CIAETGITAHG--VTLLSSLKTLVFLDLGGLA-VTDQALSSLQVLTKLQYLDLWGSKISN 204
            +    +      + L      L +L+L     +TD A+  +  +  L  +DL G+ ISN
Sbjct: 536 NLTNCSLLGDSSVIRLSERCPNLHYLNLRNCEHLTDLAIEYIASMLSLISVDLSGTLISN 595

Query: 205 TGSDVLQMFPKLNFLNIA-WTNVTKFPNLPH------LESLNVSNC--VIDSTIK 245
            G  +L    KL  ++++   N+T F    +      LE L+VS C  + D  IK
Sbjct: 596 EGMTILSRHRKLREVSVSDCVNITDFGIRAYCKTSLLLEHLDVSYCSQLTDDIIK 650

[Close]

BLAST of MC10g0745 vs. NCBI nr
Match: XP_022136501.1 (F-box/LRR-repeat protein 14 isoform X1 [Momordica charantia])

HSP 1 Score: 1142 bits (2954), Expect = 0.0
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 0


Query: 1   MENKLVEFCINSACESKDSVEKWRRQKRSLERLPSQLADALLRRLHARRLLYPSILEVFK 60
           MENKLVEFCINSACESKDSVEKWRRQKRSLERLPSQLADALLRRLHARRLLYPSILEVFK
Sbjct: 1   MENKLVEFCINSACESKDSVEKWRRQKRSLERLPSQLADALLRRLHARRLLYPSILEVFK 60

Query: 61  FTVEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSR 120
           FTVEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSR
Sbjct: 61  FTVEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSR 120

Query: 121 CSKVTDAGIRHLLSIPTLEKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSL 180
           CSKVTDAGIRHLLSIPTLEKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSL
Sbjct: 121 CSKVTDAGIRHLLSIPTLEKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSL 180

Query: 181 QVLTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVID 240
           QVLTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVID
Sbjct: 181 QVLTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVID 240

Query: 241 STIKGSGAKVPLRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALE 300
           STIKGSGAKVPLRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALE
Sbjct: 241 STIKGSGAKVPLRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALE 300

Query: 301 HLDLSSTAIGDNSAGLIASVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMID 360
           HLDLSSTAIGDNSAGLIASVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMID
Sbjct: 301 HLDLSSTAIGDNSAGLIASVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMID 360

Query: 361 DFAISYISLMPGLKSIDLSETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVD 420
           DFAISYISLMPGLKSIDLSETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVD
Sbjct: 361 DFAISYISLMPGLKSIDLSETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVD 420

Query: 421 DESLRPLSRFRRLSHLLLRSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAAT 480
           DESLRPLSRFRRLSHLLLRSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAAT
Sbjct: 421 DESLRPLSRFRRLSHLLLRSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAAT 480

Query: 481 LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQR 540
           LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQR
Sbjct: 481 LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQR 540

Query: 541 NKSAAMSPYFVDQRLKYSSEELLALQFSSLSHGSTSTREIGQKSDT 586
           NKSAAMSPYFVDQRLKYSSEELLALQFSSLSHGSTSTREIGQKSDT
Sbjct: 541 NKSAAMSPYFVDQRLKYSSEELLALQFSSLSHGSTSTREIGQKSDT 586

[Close]

BLAST of MC10g0745 vs. NCBI nr
Match: KAG6600520.1 (hypothetical protein SDJN03_05753, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031160.1 inlI [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 994 bits (2569), Expect = 0.0
Identity = 506/586 (86.35%), Postives = 539/586 (91.98%), Query Frame = 0


Query: 1   MENKLVEFCINSACESKDSVEKWRRQKRSLERLPSQLADALLRRLHARRLLYPSILEVFK 60
           MEN+LVE C+NSACESKDSVEKWRRQKRSLERLPS LADALLRRLHARRLLYPS+LEV K
Sbjct: 3   MENQLVELCLNSACESKDSVEKWRRQKRSLERLPSHLADALLRRLHARRLLYPSLLEVVK 62

Query: 61  FTVEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSR 120
           +T+EVVDLSGENAVDSEWMAY+GA+RYLRSLN+SNCHRLSSSGIWA+AGMTTLRELN+SR
Sbjct: 63  YTIEVVDLSGENAVDSEWMAYIGAFRYLRSLNVSNCHRLSSSGIWAVAGMTTLRELNISR 122

Query: 121 CSKVTDAGIRHLLSIPTLEKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSL 180
           C KVTDAGIRHLLSIPTLEKLCIAETGITA GVTLLSSLKTL+FLDLGGL VTDQALSSL
Sbjct: 123 CLKVTDAGIRHLLSIPTLEKLCIAETGITARGVTLLSSLKTLMFLDLGGLPVTDQALSSL 182

Query: 181 QVLTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVID 240
           QVLTKL +LDLWGSKIS++GSDVLQMFPKLNFLNIAWTNVTKFPNLPHLE LN+SNC+ID
Sbjct: 183 QVLTKLHHLDLWGSKISSSGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLECLNMSNCIID 242

Query: 241 STIKGSGAKVPLRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALE 300
           ST+KG G KVPL+KLIASGATFSNETEDL FVGTSFL HLDFSNA LHRFCFL HMKALE
Sbjct: 243 STLKGPGDKVPLKKLIASGATFSNETEDLRFVGTSFLYHLDFSNASLHRFCFLTHMKALE 302

Query: 301 HLDLSSTAIGDNSAGLIASVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMID 360
           +LDLSST IGDNS  LIASVGENLK+LNLSCTTVSSSGVGILAGKVP LETLSLSHTMID
Sbjct: 303 YLDLSSTTIGDNSIKLIASVGENLKNLNLSCTTVSSSGVGILAGKVPKLETLSLSHTMID 362

Query: 361 DFAISYISLMPGLKSIDLSETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVD 420
           D A+SYIS MP LKSIDLSETDIKGYI+LSAA G+E+LS T L+ LDCLE+LNLE THVD
Sbjct: 363 DVALSYISTMPSLKSIDLSETDIKGYIHLSAAVGSEFLSLTKLRTLDCLEMLNLEHTHVD 422

Query: 421 DESLRPLSRFRRLSHLLLRSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAAT 480
           D SLRPLS+F RLSHLL+RSPS TDVSLSYLS LP L   SIRDAVLTNQAFD LKPAAT
Sbjct: 423 DGSLRPLSKFPRLSHLLIRSPSFTDVSLSYLSSLPNLKTFSIRDAVLTNQAFDFLKPAAT 482

Query: 481 LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQR 540
           LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHF SD TSS RPSTH TPKKL+LNQR
Sbjct: 483 LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFLSDPTSSSRPSTHLTPKKLRLNQR 542

Query: 541 NKSAAMSPYFVDQRLKYSSEELLALQFSSLSHGSTSTREIGQKSDT 586
           N+S   SPYFVDQRLKYS EELLALQFSSL+H STST E  +KSD+
Sbjct: 543 NRSTVTSPYFVDQRLKYSREELLALQFSSLAHSSTSTPETSRKSDS 588

[Close]

BLAST of MC10g0745 vs. NCBI nr
Match: XP_022136502.1 (F-box/LRR-repeat protein 14 isoform X2 [Momordica charantia] >XP_022136504.1 F-box/LRR-repeat protein 14 isoform X2 [Momordica charantia])

HSP 1 Score: 993 bits (2567), Expect = 0.0
Identity = 508/508 (100.00%), Postives = 508/508 (100.00%), Query Frame = 0


Query: 79  MAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSRCSKVTDAGIRHLLSIPTL 138
           MAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSRCSKVTDAGIRHLLSIPTL
Sbjct: 1   MAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSRCSKVTDAGIRHLLSIPTL 60

Query: 139 EKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSLQVLTKLQYLDLWGSKISN 198
           EKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSLQVLTKLQYLDLWGSKISN
Sbjct: 61  EKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSLQVLTKLQYLDLWGSKISN 120

Query: 199 TGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVIDSTIKGSGAKVPLRKLIAS 258
           TGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVIDSTIKGSGAKVPLRKLIAS
Sbjct: 121 TGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVIDSTIKGSGAKVPLRKLIAS 180

Query: 259 GATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALEHLDLSSTAIGDNSAGLIA 318
           GATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALEHLDLSSTAIGDNSAGLIA
Sbjct: 181 GATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALEHLDLSSTAIGDNSAGLIA 240

Query: 319 SVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMIDDFAISYISLMPGLKSIDL 378
           SVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMIDDFAISYISLMPGLKSIDL
Sbjct: 241 SVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMIDDFAISYISLMPGLKSIDL 300

Query: 379 SETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVDDESLRPLSRFRRLSHLLL 438
           SETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVDDESLRPLSRFRRLSHLLL
Sbjct: 301 SETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVDDESLRPLSRFRRLSHLLL 360

Query: 439 RSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAATLQKIDLRGCWLLTEDGLL 498
           RSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAATLQKIDLRGCWLLTEDGLL
Sbjct: 361 RSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAATLQKIDLRGCWLLTEDGLL 420

Query: 499 EFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQRNKSAAMSPYFVDQRLKYS 558
           EFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQRNKSAAMSPYFVDQRLKYS
Sbjct: 421 EFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQRNKSAAMSPYFVDQRLKYS 480

Query: 559 SEELLALQFSSLSHGSTSTREIGQKSDT 586
           SEELLALQFSSLSHGSTSTREIGQKSDT
Sbjct: 481 SEELLALQFSSLSHGSTSTREIGQKSDT 508

[Close]

BLAST of MC10g0745 vs. NCBI nr
Match: XP_022942088.1 (F-box/LRR-repeat protein 14 isoform X1 [Cucurbita moschata] >XP_022942089.1 F-box/LRR-repeat protein 14 isoform X1 [Cucurbita moschata] >XP_022942090.1 F-box/LRR-repeat protein 14 isoform X1 [Cucurbita moschata])

HSP 1 Score: 990 bits (2560), Expect = 0.0
Identity = 505/586 (86.18%), Postives = 539/586 (91.98%), Query Frame = 0


Query: 1   MENKLVEFCINSACESKDSVEKWRRQKRSLERLPSQLADALLRRLHARRLLYPSILEVFK 60
           MEN+LVE C+NSACESKDSVEKWRRQKRSLERLPS LADALLRRLHARRLLYPS+LEV K
Sbjct: 3   MENQLVELCLNSACESKDSVEKWRRQKRSLERLPSHLADALLRRLHARRLLYPSLLEVVK 62

Query: 61  FTVEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSR 120
           +T+EVVDLSGENAVDSEWMAY+GA+RYLRSLN+SNCHRLSSSGIWA+AGMTTLRELN+SR
Sbjct: 63  YTIEVVDLSGENAVDSEWMAYIGAFRYLRSLNVSNCHRLSSSGIWAVAGMTTLRELNISR 122

Query: 121 CSKVTDAGIRHLLSIPTLEKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSL 180
           C KVTDAGIRHLLSIPTLEKLCIAETGITA GVTLLSSLKTL+FLDLGGL VTDQALSSL
Sbjct: 123 CLKVTDAGIRHLLSIPTLEKLCIAETGITARGVTLLSSLKTLMFLDLGGLPVTDQALSSL 182

Query: 181 QVLTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVID 240
           QVLTKL +LDLWGSKIS++GSDVLQMFPKLNFLNIAWTNVTKFPNLPHLE LN+SNC+ID
Sbjct: 183 QVLTKLHHLDLWGSKISSSGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLECLNMSNCIID 242

Query: 241 STIKGSGAKVPLRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALE 300
           ST+KG G KVPL+KLIASGATFSNETEDL FVGTSFL HLDFSNA LHRFCFL  MKALE
Sbjct: 243 STLKGPGDKVPLKKLIASGATFSNETEDLGFVGTSFLYHLDFSNASLHRFCFLTDMKALE 302

Query: 301 HLDLSSTAIGDNSAGLIASVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMID 360
           +LDLSST IGDNS  LIASVGENLK+LNLSCTTVSSSGV ILAGK+P LETLSLSHTMID
Sbjct: 303 YLDLSSTTIGDNSIKLIASVGENLKNLNLSCTTVSSSGVRILAGKLPKLETLSLSHTMID 362

Query: 361 DFAISYISLMPGLKSIDLSETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVD 420
           D A+SYIS MP LKSIDLSETDIKGYI+LSAAEG+E+LS T L+ LDCLE+LNLE THVD
Sbjct: 363 DVALSYISTMPSLKSIDLSETDIKGYIHLSAAEGSEFLSLTKLRTLDCLEMLNLEHTHVD 422

Query: 421 DESLRPLSRFRRLSHLLLRSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAAT 480
           D SLRPLS+F RLSHLL+RSPS TDVSLSYLSILP L   SIRDAVLTNQAFD LKPAAT
Sbjct: 423 DGSLRPLSKFPRLSHLLIRSPSFTDVSLSYLSILPNLKTFSIRDAVLTNQAFDFLKPAAT 482

Query: 481 LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQR 540
           LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHF SD TSS+RPSTH TPKKL+LNQR
Sbjct: 483 LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFLSDPTSSNRPSTHLTPKKLRLNQR 542

Query: 541 NKSAAMSPYFVDQRLKYSSEELLALQFSSLSHGSTSTREIGQKSDT 586
           N+S   SPYFVDQRLKYS EELLALQFSSL+H STST E   KSD+
Sbjct: 543 NRSTVTSPYFVDQRLKYSREELLALQFSSLAHSSTSTPETSHKSDS 588

[Close]

BLAST of MC10g0745 vs. NCBI nr
Match: XP_023533280.1 (F-box/LRR-repeat protein 14 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023533289.1 F-box/LRR-repeat protein 14 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023533296.1 F-box/LRR-repeat protein 14 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 990 bits (2559), Expect = 0.0
Identity = 505/586 (86.18%), Postives = 538/586 (91.81%), Query Frame = 0


Query: 1   MENKLVEFCINSACESKDSVEKWRRQKRSLERLPSQLADALLRRLHARRLLYPSILEVFK 60
           MEN+LVE C+NSACESKDSVEKWRRQKRSLERLPS LADALLRRLHARRLLYPS+LEV K
Sbjct: 3   MENQLVELCLNSACESKDSVEKWRRQKRSLERLPSHLADALLRRLHARRLLYPSLLEVVK 62

Query: 61  FTVEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSR 120
           +T+EVVDLSGENAVDSEWMAY+GA+RYLRSLN+SNCHRLSSSGIWA+AGMTTLRELN+SR
Sbjct: 63  YTIEVVDLSGENAVDSEWMAYIGAFRYLRSLNVSNCHRLSSSGIWAVAGMTTLRELNISR 122

Query: 121 CSKVTDAGIRHLLSIPTLEKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSL 180
           C KVTDAGIRHLLSIPTLEKLCIAETGITA GVTLLSSLKTL+FLDLGGL VTDQALSSL
Sbjct: 123 CLKVTDAGIRHLLSIPTLEKLCIAETGITARGVTLLSSLKTLMFLDLGGLPVTDQALSSL 182

Query: 181 QVLTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVID 240
           QVLTKL +LDLWGSKIS++GSDVLQMFPKLNFLNIAWTNVTKFPNL HLE LN+SNC+ID
Sbjct: 183 QVLTKLHHLDLWGSKISSSGSDVLQMFPKLNFLNIAWTNVTKFPNLSHLECLNMSNCIID 242

Query: 241 STIKGSGAKVPLRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALE 300
           ST+KG G KVPL+KLIASGATFSNETEDL FVGTSFL HLDFSNA LHRFCFL H KALE
Sbjct: 243 STLKGPGDKVPLKKLIASGATFSNETEDLGFVGTSFLYHLDFSNASLHRFCFLSHTKALE 302

Query: 301 HLDLSSTAIGDNSAGLIASVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMID 360
           +LDLSST IGDNS  LIASVGENLK+LNLSCTTVSSSGVGILAGKVP LETLSLSHTMID
Sbjct: 303 YLDLSSTTIGDNSIELIASVGENLKNLNLSCTTVSSSGVGILAGKVPKLETLSLSHTMID 362

Query: 361 DFAISYISLMPGLKSIDLSETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVD 420
           D A+SYIS MP LKSIDLSETDIKGYI+ SAAEG+E+LS T L+ LDCLE+LNLE THVD
Sbjct: 363 DVALSYISTMPSLKSIDLSETDIKGYIHPSAAEGSEFLSLTKLRTLDCLEMLNLEHTHVD 422

Query: 421 DESLRPLSRFRRLSHLLLRSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAAT 480
           D SLRPLS+F RLSHLL+RSPS TDVSLSYLSILP L   SIRDAVLTNQAFD LKPAAT
Sbjct: 423 DGSLRPLSKFPRLSHLLIRSPSFTDVSLSYLSILPNLKTFSIRDAVLTNQAFDFLKPAAT 482

Query: 481 LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQR 540
           LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHF SD TSS+RPSTH TPKKL+LNQR
Sbjct: 483 LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFLSDPTSSNRPSTHLTPKKLRLNQR 542

Query: 541 NKSAAMSPYFVDQRLKYSSEELLALQFSSLSHGSTSTREIGQKSDT 586
           N+S   SPYFVDQRLKYS EELLALQFSSL+H STST E   KSD+
Sbjct: 543 NRSTVTSPYFVDQRLKYSREELLALQFSSLAHSSTSTPETSHKSDS 588

[Close]

BLAST of MC10g0745 vs. ExPASy TrEMBL
Match: A0A6J1C3P0 (F-box/LRR-repeat protein 14 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008197 PE=4 SV=1)

HSP 1 Score: 1142 bits (2954), Expect = 0.0
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 0


Query: 1   MENKLVEFCINSACESKDSVEKWRRQKRSLERLPSQLADALLRRLHARRLLYPSILEVFK 60
           MENKLVEFCINSACESKDSVEKWRRQKRSLERLPSQLADALLRRLHARRLLYPSILEVFK
Sbjct: 1   MENKLVEFCINSACESKDSVEKWRRQKRSLERLPSQLADALLRRLHARRLLYPSILEVFK 60

Query: 61  FTVEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSR 120
           FTVEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSR
Sbjct: 61  FTVEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSR 120

Query: 121 CSKVTDAGIRHLLSIPTLEKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSL 180
           CSKVTDAGIRHLLSIPTLEKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSL
Sbjct: 121 CSKVTDAGIRHLLSIPTLEKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSL 180

Query: 181 QVLTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVID 240
           QVLTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVID
Sbjct: 181 QVLTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVID 240

Query: 241 STIKGSGAKVPLRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALE 300
           STIKGSGAKVPLRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALE
Sbjct: 241 STIKGSGAKVPLRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALE 300

Query: 301 HLDLSSTAIGDNSAGLIASVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMID 360
           HLDLSSTAIGDNSAGLIASVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMID
Sbjct: 301 HLDLSSTAIGDNSAGLIASVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMID 360

Query: 361 DFAISYISLMPGLKSIDLSETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVD 420
           DFAISYISLMPGLKSIDLSETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVD
Sbjct: 361 DFAISYISLMPGLKSIDLSETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVD 420

Query: 421 DESLRPLSRFRRLSHLLLRSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAAT 480
           DESLRPLSRFRRLSHLLLRSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAAT
Sbjct: 421 DESLRPLSRFRRLSHLLLRSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAAT 480

Query: 481 LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQR 540
           LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQR
Sbjct: 481 LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQR 540

Query: 541 NKSAAMSPYFVDQRLKYSSEELLALQFSSLSHGSTSTREIGQKSDT 586
           NKSAAMSPYFVDQRLKYSSEELLALQFSSLSHGSTSTREIGQKSDT
Sbjct: 541 NKSAAMSPYFVDQRLKYSSEELLALQFSSLSHGSTSTREIGQKSDT 586

[Close]

BLAST of MC10g0745 vs. ExPASy TrEMBL
Match: A0A6J1C4H3 (F-box/LRR-repeat protein 14 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008197 PE=4 SV=1)

HSP 1 Score: 993 bits (2567), Expect = 0.0
Identity = 508/508 (100.00%), Postives = 508/508 (100.00%), Query Frame = 0


Query: 79  MAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSRCSKVTDAGIRHLLSIPTL 138
           MAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSRCSKVTDAGIRHLLSIPTL
Sbjct: 1   MAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSRCSKVTDAGIRHLLSIPTL 60

Query: 139 EKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSLQVLTKLQYLDLWGSKISN 198
           EKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSLQVLTKLQYLDLWGSKISN
Sbjct: 61  EKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSLQVLTKLQYLDLWGSKISN 120

Query: 199 TGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVIDSTIKGSGAKVPLRKLIAS 258
           TGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVIDSTIKGSGAKVPLRKLIAS
Sbjct: 121 TGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVIDSTIKGSGAKVPLRKLIAS 180

Query: 259 GATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALEHLDLSSTAIGDNSAGLIA 318
           GATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALEHLDLSSTAIGDNSAGLIA
Sbjct: 181 GATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALEHLDLSSTAIGDNSAGLIA 240

Query: 319 SVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMIDDFAISYISLMPGLKSIDL 378
           SVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMIDDFAISYISLMPGLKSIDL
Sbjct: 241 SVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMIDDFAISYISLMPGLKSIDL 300

Query: 379 SETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVDDESLRPLSRFRRLSHLLL 438
           SETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVDDESLRPLSRFRRLSHLLL
Sbjct: 301 SETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVDDESLRPLSRFRRLSHLLL 360

Query: 439 RSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAATLQKIDLRGCWLLTEDGLL 498
           RSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAATLQKIDLRGCWLLTEDGLL
Sbjct: 361 RSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAATLQKIDLRGCWLLTEDGLL 420

Query: 499 EFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQRNKSAAMSPYFVDQRLKYS 558
           EFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQRNKSAAMSPYFVDQRLKYS
Sbjct: 421 EFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQRNKSAAMSPYFVDQRLKYS 480

Query: 559 SEELLALQFSSLSHGSTSTREIGQKSDT 586
           SEELLALQFSSLSHGSTSTREIGQKSDT
Sbjct: 481 SEELLALQFSSLSHGSTSTREIGQKSDT 508

[Close]

BLAST of MC10g0745 vs. ExPASy TrEMBL
Match: A0A6J1FQB0 (F-box/LRR-repeat protein 14 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447267 PE=4 SV=1)

HSP 1 Score: 990 bits (2560), Expect = 0.0
Identity = 505/586 (86.18%), Postives = 539/586 (91.98%), Query Frame = 0


Query: 1   MENKLVEFCINSACESKDSVEKWRRQKRSLERLPSQLADALLRRLHARRLLYPSILEVFK 60
           MEN+LVE C+NSACESKDSVEKWRRQKRSLERLPS LADALLRRLHARRLLYPS+LEV K
Sbjct: 3   MENQLVELCLNSACESKDSVEKWRRQKRSLERLPSHLADALLRRLHARRLLYPSLLEVVK 62

Query: 61  FTVEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSR 120
           +T+EVVDLSGENAVDSEWMAY+GA+RYLRSLN+SNCHRLSSSGIWA+AGMTTLRELN+SR
Sbjct: 63  YTIEVVDLSGENAVDSEWMAYIGAFRYLRSLNVSNCHRLSSSGIWAVAGMTTLRELNISR 122

Query: 121 CSKVTDAGIRHLLSIPTLEKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSL 180
           C KVTDAGIRHLLSIPTLEKLCIAETGITA GVTLLSSLKTL+FLDLGGL VTDQALSSL
Sbjct: 123 CLKVTDAGIRHLLSIPTLEKLCIAETGITARGVTLLSSLKTLMFLDLGGLPVTDQALSSL 182

Query: 181 QVLTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVID 240
           QVLTKL +LDLWGSKIS++GSDVLQMFPKLNFLNIAWTNVTKFPNLPHLE LN+SNC+ID
Sbjct: 183 QVLTKLHHLDLWGSKISSSGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLECLNMSNCIID 242

Query: 241 STIKGSGAKVPLRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALE 300
           ST+KG G KVPL+KLIASGATFSNETEDL FVGTSFL HLDFSNA LHRFCFL  MKALE
Sbjct: 243 STLKGPGDKVPLKKLIASGATFSNETEDLGFVGTSFLYHLDFSNASLHRFCFLTDMKALE 302

Query: 301 HLDLSSTAIGDNSAGLIASVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMID 360
           +LDLSST IGDNS  LIASVGENLK+LNLSCTTVSSSGV ILAGK+P LETLSLSHTMID
Sbjct: 303 YLDLSSTTIGDNSIKLIASVGENLKNLNLSCTTVSSSGVRILAGKLPKLETLSLSHTMID 362

Query: 361 DFAISYISLMPGLKSIDLSETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVD 420
           D A+SYIS MP LKSIDLSETDIKGYI+LSAAEG+E+LS T L+ LDCLE+LNLE THVD
Sbjct: 363 DVALSYISTMPSLKSIDLSETDIKGYIHLSAAEGSEFLSLTKLRTLDCLEMLNLEHTHVD 422

Query: 421 DESLRPLSRFRRLSHLLLRSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAAT 480
           D SLRPLS+F RLSHLL+RSPS TDVSLSYLSILP L   SIRDAVLTNQAFD LKPAAT
Sbjct: 423 DGSLRPLSKFPRLSHLLIRSPSFTDVSLSYLSILPNLKTFSIRDAVLTNQAFDFLKPAAT 482

Query: 481 LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQR 540
           LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHF SD TSS+RPSTH TPKKL+LNQR
Sbjct: 483 LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFLSDPTSSNRPSTHLTPKKLRLNQR 542

Query: 541 NKSAAMSPYFVDQRLKYSSEELLALQFSSLSHGSTSTREIGQKSDT 586
           N+S   SPYFVDQRLKYS EELLALQFSSL+H STST E   KSD+
Sbjct: 543 NRSTVTSPYFVDQRLKYSREELLALQFSSLAHSSTSTPETSHKSDS 588

[Close]

BLAST of MC10g0745 vs. ExPASy TrEMBL
Match: A0A6J1IUQ8 (F-box/LRR-repeat protein 14 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479466 PE=4 SV=1)

HSP 1 Score: 986 bits (2550), Expect = 0.0
Identity = 503/586 (85.84%), Postives = 538/586 (91.81%), Query Frame = 0


Query: 1   MENKLVEFCINSACESKDSVEKWRRQKRSLERLPSQLADALLRRLHARRLLYPSILEVFK 60
           MEN+LVE C+NSACESKDSVEKWRRQKRSLERLPS LADALLRRLHARRLLYPS+LEV K
Sbjct: 3   MENQLVELCLNSACESKDSVEKWRRQKRSLERLPSHLADALLRRLHARRLLYPSLLEVVK 62

Query: 61  FTVEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSR 120
           +T+EVVDLSGENAVDSEWMAY+GA+RYLRSLN+SNCHRLSSSGIWA+AGMTTLRELN+SR
Sbjct: 63  YTIEVVDLSGENAVDSEWMAYIGAFRYLRSLNISNCHRLSSSGIWAVAGMTTLRELNISR 122

Query: 121 CSKVTDAGIRHLLSIPTLEKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSL 180
           C KVTDAGIRHLLSIPTLEKLCIAETGITA GVTLLSSLKTL+FLDLGGL VTDQALSSL
Sbjct: 123 CLKVTDAGIRHLLSIPTLEKLCIAETGITARGVTLLSSLKTLMFLDLGGLPVTDQALSSL 182

Query: 181 QVLTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVID 240
           QVLTKL +LDLWGSKIS++GSDVLQMFPKLNFLNIAWTNVTKFPNLPHLE LN+SNC+ID
Sbjct: 183 QVLTKLHHLDLWGSKISSSGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLECLNMSNCIID 242

Query: 241 STIKGSGAKVPLRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALE 300
           ST+KG G  VPL+KLIASGATFSNETEDL FVGTSFL HLDFSNA LHRF FL HMKALE
Sbjct: 243 STLKGPGDNVPLKKLIASGATFSNETEDLGFVGTSFLYHLDFSNASLHRFGFLTHMKALE 302

Query: 301 HLDLSSTAIGDNSAGLIASVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMID 360
           +LDLSST IGDNS  L+ASVGENLK+LNLSCTTVSSSGVGILAGKVP LETLSLSHTMID
Sbjct: 303 YLDLSSTTIGDNSIELLASVGENLKNLNLSCTTVSSSGVGILAGKVPKLETLSLSHTMID 362

Query: 361 DFAISYISLMPGLKSIDLSETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVD 420
           D A+SYIS MP LKSIDLSETDIKGYI+LSAAEG+E+LS T L+ LDCLE+LNLE THVD
Sbjct: 363 DVALSYISTMPSLKSIDLSETDIKGYIHLSAAEGSEFLSLTKLRTLDCLEMLNLEHTHVD 422

Query: 421 DESLRPLSRFRRLSHLLLRSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAAT 480
           D SLRPLS+F RLSHLL+RSPS TDVSLSYLSILPKL   SIRDAVLTNQAFD LKPAAT
Sbjct: 423 DGSLRPLSKFPRLSHLLIRSPSFTDVSLSYLSILPKLKTFSIRDAVLTNQAFDFLKPAAT 482

Query: 481 LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQR 540
           LQ+IDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHF SD TSS+RPSTH TPKKL+LNQR
Sbjct: 483 LQRIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFLSDPTSSNRPSTHLTPKKLRLNQR 542

Query: 541 NKSAAMSPYFVDQRLKYSSEELLALQFSSLSHGSTSTREIGQKSDT 586
           N+S   SPYFVDQRLKYS EELLALQFSSL+  ST T E   KSD+
Sbjct: 543 NRSTVTSPYFVDQRLKYSREELLALQFSSLAQSSTYTPETSHKSDS 588

[Close]

BLAST of MC10g0745 vs. ExPASy TrEMBL
Match: A0A5A7TU43 (F-box/LRR-repeat protein 14 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G001990 PE=4 SV=1)

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 490/586 (83.62%), Postives = 529/586 (90.27%), Query Frame = 0


Query: 1   MENKLVEFCINSACESKDSVEKWRRQKRSLERLPSQLADALLRRLHARRLLYPSILEVFK 60
           MEN LVE CINSAC  K+SVEKWRRQKRSLERLPS LADALLRRLHARRLLYPS+LEVFK
Sbjct: 1   MENHLVELCINSACRCKESVEKWRRQKRSLERLPSHLADALLRRLHARRLLYPSLLEVFK 60

Query: 61  FTVEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSR 120
           +T+EVVDLSGENAVDSEWMAYLG++RYL++LN+SNCHRLSSSGIWAI+GMTTLRELN+SR
Sbjct: 61  YTIEVVDLSGENAVDSEWMAYLGSFRYLQALNVSNCHRLSSSGIWAISGMTTLRELNVSR 120

Query: 121 CSKVTDAGIRHLLSIPTLEKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSL 180
           C KVTDAGIRHLLSIPTLEKLCIAETGITAHGV L+SSLKTLVFLDLGGL VTDQALSSL
Sbjct: 121 CLKVTDAGIRHLLSIPTLEKLCIAETGITAHGVALISSLKTLVFLDLGGLPVTDQALSSL 180

Query: 181 QVLTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVID 240
           QVLTKLQYLDLWGSKISN+GSDVLQMFPKL+FLNIAWTNVTKFPNLPHLE LN+SNC+ID
Sbjct: 181 QVLTKLQYLDLWGSKISNSGSDVLQMFPKLSFLNIAWTNVTKFPNLPHLECLNMSNCIID 240

Query: 241 STIKGSGAKVPLRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALE 300
           ST+KG GAKVPLRKLIASGATFSNETEDL FV    L +LDFSNA LHRFCFL HMKA+E
Sbjct: 241 STLKGLGAKVPLRKLIASGATFSNETEDLGFVAMDALYYLDFSNASLHRFCFLSHMKAVE 300

Query: 301 HLDLSSTAIGDNSAGLIASVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMID 360
           HLDLSST IGD+S  LIAS+GENLK+LNLSCT VSSSG+G LAGKV NL TLSLSHTM+D
Sbjct: 301 HLDLSSTTIGDSSVELIASIGENLKYLNLSCTAVSSSGIGSLAGKVSNLATLSLSHTMVD 360

Query: 361 DFAISYISLMPGLKSIDLSETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHVD 420
           D A+SY+S+MP LK IDLSETDIKGYI+LS  E  +  S T+LQNLDCLE+LNLE THVD
Sbjct: 361 DDALSYMSMMPSLKCIDLSETDIKGYIHLSTPETVKIFSLTELQNLDCLEMLNLEHTHVD 420

Query: 421 DESLRPLSRFRRLSHLLLRSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAAT 480
           DESLR LSRFR+LSHL+LRSPS TD  LSYLSILP L  LSIRDAVLTNQAFD LKP AT
Sbjct: 421 DESLRSLSRFRKLSHLMLRSPSFTDTVLSYLSILPNLKTLSIRDAVLTNQAFDTLKPVAT 480

Query: 481 LQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFSSDQTSSDRPSTHFTPKKLQLNQR 540
           LQKIDLRGCWLLTE GL  FHRRFPQIEVRHELFHFSS+ TS+DRPSTHF PKK+QLNQ 
Sbjct: 481 LQKIDLRGCWLLTEGGLSVFHRRFPQIEVRHELFHFSSNPTSTDRPSTHFIPKKVQLNQT 540

Query: 541 NKSAAMSPYFVDQRLKYSSEELLALQFSSLSHGSTSTREIGQKSDT 586
           ++S  MSPYFVDQRLKYS EELLALQFSSLSHGSTS  E G+KSDT
Sbjct: 541 SRSTGMSPYFVDQRLKYSKEELLALQFSSLSHGSTSVPETGRKSDT 586

[Close]

BLAST of MC10g0745 vs. TAIR 10
Match: AT4G23840.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 531.2 bits (1367), Expect = 1.1e-150
Identity = 294/577 (50.95%), Postives = 396/577 (68.63%), Query Frame = 0


Query: 1   MENKLVEFCINSACESKDSVEKWRRQKRSLERLPSQLADALLRRLHARRLLYPSILEVFK 60
           ME+ LV  C+  AC+S D+VE+WR Q+RSLE LP  LADALLRRL  +RLL+PS+LE FK
Sbjct: 1   MESPLVRLCLKEACKSGDAVERWRLQRRSLESLPPHLADALLRRLLKKRLLFPSLLEGFK 60

Query: 61  FTVEVVDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSR 120
           ++VE +DL G+++V++EWMAY+G +  L +LNLS+C R++SS +W I G+T+L EL+LSR
Sbjct: 61  YSVENIDLRGKSSVNAEWMAYIGGFVNLITLNLSDCQRINSSTLWPITGLTSLTELDLSR 120

Query: 121 CSKVTDAGIRHLLSIPTLEKLCIAETGITAHGVTLLSSLKTLVFLDLGGLAVTDQALSSL 180
           C KVTDAG++HL S+  L+KL I++TG+T  G++LL+SLK L  LDLGGL VTDQ L SL
Sbjct: 121 CFKVTDAGMKHLQSVVNLKKLWISQTGVTEVGISLLASLKKLSLLDLGGLPVTDQNLISL 180

Query: 181 QVLTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVID 240
           Q LTKL+YLD+WGS ++N G+  +  F  L+FLN++WT++T+ PN+PHLE L+++ C I 
Sbjct: 181 QALTKLEYLDIWGSNVTNQGAVSILKFSNLSFLNLSWTSITQTPNIPHLECLHMNTCTIV 240

Query: 241 STIKGSGAKVPLRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKALE 300
           S  K   +   L+KL+ SGA FS ETE L F   S + +LD S   L  F FL  M  LE
Sbjct: 241 SEPKTHSSLASLKKLVLSGANFSAETESLSFTNKSCITYLDVSKTSLKNFSFLETMFNLE 300

Query: 301 HLDLSSTAIGDNSAGLIASVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSHTMID 360
           HLDLSSTA GD+S G +A VGENLK+LN+S T ++ SGVG LAG VP LETLS+S T +D
Sbjct: 301 HLDLSSTAFGDDSVGFVACVGENLKNLNVSDTQITPSGVGNLAGHVPQLETLSMSQTFVD 360

Query: 361 DFAISYIS-LMPGLKSIDLSETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLERTHV 420
           D +I  IS  MP +K++DL      G+ YL + +  +  S   LQ+L  LE L+LE  ++
Sbjct: 361 DLSILLISTTMPCIKALDLGMNSTLGFYYLISPQEEKEKSLAALQSLTSLETLSLEHPYL 420

Query: 421 DDESLRPLSRFRRLSHLLLRSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLKPAA 480
            D++L  LS    L+HL L S SLTD +L +LS LP L  L +RD VLT+   +  +P  
Sbjct: 421 GDKALSGLSSLTGLTHLSLTSTSLTDSTLHHLSSLPNLVSLGVRDGVLTSNGLEKFRPPN 480

Query: 481 TLQKIDLRGCWLLTEDGLLEFHRRFPQIEVRHELFHFSS-DQTS-SDRPST--HFTPKKL 540
            L+ +DL+GCWLLT+D +    +R+P I+VRHE    SS DQ     R ST   F     
Sbjct: 481 RLRTLDLQGCWLLTKDDIAGLCKRYPHIKVRHEHADSSSLDQNQLLPRSSTPQSFGKVAR 540

Query: 541 QLNQRNKSAAMSP-YFVDQRLKYSSEELLALQFSSLS 572
           + NQR +S+   P  F+DQR+KY+ EEL+ALQ S LS
Sbjct: 541 RNNQRPESSVAVPRSFLDQRVKYNREELVALQNSPLS 577

[Close]

BLAST of MC10g0745 vs. TAIR 10
Match: AT1G15740.1 (Leucine-rich repeat family protein )

HSP 1 Score: 118.6 bits (296), Expect = 1.7e-26
Identity = 117/439 (26.65%), Postives = 206/439 (46.92%), Query Frame = 0


Query: 66  VDLSGENAVDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSRCSKVT 125
           VD SG +  DS  ++  G    L SLN + C ++S+ G+  ++G++ L  L+  R + +T
Sbjct: 143 VDFSGSDITDSGLVSLKGCTN-LESLNFNFCDQISNRGLVHLSGLSNLTSLSFRRNAAIT 202

Query: 126 DAGIRHLLSIPTLEKLCIAETGITAHGVTLLSSLKTLVFLDLGGL-AVTDQALSSLQVLT 185
             G+R L ++  L+KL + +      G+  L +L  L  L++     +TD  +  L VLT
Sbjct: 203 AQGMRALSNLVNLKKLDLEKCPGIDGGLVHLRALTKLESLNIKWCNCITDADMEPLSVLT 262

Query: 186 KLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTN------VTKFPNLPHLESLNVSNCV 245
            L+ L +  SKI++ G   L+   KLN LN+          +     L  L  LN++ C 
Sbjct: 263 NLRSLQICCSKITDIGISYLKGLNKLNLLNLEGCRHVTAACLDTLTALAGLMYLNLNRCN 322

Query: 246 IDSTIKGSGAKVPLRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACL--HRFCFLPHM 305
              +     + +   K++  G      +  +   G + L  L+  +  +       L  M
Sbjct: 323 FSDSGCEKFSDLINLKILNLGMNNITNSCLVHLKGLTKLESLNLDSCRIGDEGLVHLSGM 382

Query: 306 KALEHLDLSSTAIGDNSAGLIASVGENLKHLNLSCTTVSSSGVGILAGKVPNLETLSLSH 365
             L+ L+LS T +G N    ++ +  NL+ +NLS T V+ SG+  L+G + +L TL+L  
Sbjct: 383 LELKSLELSDTEVGSNGLRHLSGL-SNLESINLSFTVVTDSGLRKLSG-LTSLRTLNLDA 442

Query: 366 TMIDDFAISYISLMPGLKSIDLSETDIKGYIYLSAAEGNEYLSFTDLQNLDCLEILNLER 425
             + D  +S ++ + GL  +DL    I          G  +L   +L+ L  LEI     
Sbjct: 443 RHVTDAGLSALTSLTGLTHLDLFGARI-------TDSGTNHL--RNLKKLQSLEICGGGL 502

Query: 426 THVDDESLRPLSRFRRLSHLLLRSPSLTDVSLSYLSILPKLTILSIRDAVLTNQAFDLLK 485
           T    ++++ LS    L+  L ++ +LTD +L  +S L  L  L++ ++ +++     LK
Sbjct: 503 TDTGVKNIKDLSSLTLLN--LSQNSNLTDKTLELISGLTGLVSLNVSNSRVSSSGLRHLK 562

Query: 486 PAATLQKIDLRGCWLLTED 496
           P   L+ + L  C L   D
Sbjct: 563 PLKNLRSLTLESCKLSAND 567

[Close]

BLAST of MC10g0745 vs. TAIR 10
Match: AT5G27920.1 (F-box family protein )

HSP 1 Score: 50.8 bits (120), Expect = 4.2e-06
Identity = 80/339 (23.60%), Postives = 145/339 (42.77%), Query Frame = 0


Query: 74  VDSEWMAYLGAYRYLRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSRCSKVTDAGI---- 133
           V   ++ Y+   ++L+++ +   H   SS +   +   +L E+ LSRC  VTD G+    
Sbjct: 288 VSGSFLKYIKGLKHLKTIWIDGAHVSDSSLVSLSSSCRSLMEIGLSRCVDVTDIGMISLA 347

Query: 134 RHLLSIPTLEKLC---IAETGITAHGVTLLSSLKTLVFLDLGGL-AVTDQALSSL----Q 193
           R+ L++ TL   C   + +  I+A    +  S + L  L L     +T++ L SL     
Sbjct: 348 RNCLNLKTLNLACCGFVTDVAISA----VAQSCRNLGTLKLESCHLITEKGLQSLGCYSM 407

Query: 194 VLTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESLNVSNCVIDS 253
           ++ +L   D +G  +++ G +                 ++K  NL  L+ L +   + D 
Sbjct: 408 LVQELDLTDCYG--VNDRGLEY----------------ISKCSNLQRLK-LGLCTNISDK 467

Query: 254 TIKGSGAKVP--LRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACLHRFCFLPHMKAL 313
            I   G+K    L   +   A F ++       G   LN L  S  C      +  ++ L
Sbjct: 468 GIFHIGSKCSKLLELDLYRCAGFGDDGLAALSRGCKSLNRLILSYCCELTDTGVEQIRQL 527

Query: 314 E---HLDL----SSTAIGDNSAGLIASVGENLKHLNLS-CTTVSSSGVGILAGKVPNLET 373
           E   HL+L    + T +G      IAS  + L +L++  C  +  SG   LA    NL  
Sbjct: 528 ELLSHLELRGLKNITGVG---LAAIASGCKKLGYLDVKLCENIDDSGFWALAYFSKNLRQ 587

Query: 374 LSLSHTMIDDFAI----SYISLMPGLKSIDLSETDIKGY 387
           ++L +  + D A+    S +S +  +  + LS   ++G+
Sbjct: 588 INLCNCSVSDTALCMLMSNLSRVQDVDLVHLSRVTVEGF 600

[Close]

BLAST of MC10g0745 vs. TAIR 10
Match: AT5G23340.1 (RNI-like superfamily protein )

HSP 1 Score: 48.5 bits (114), Expect = 2.1e-05
Identity = 94/371 (25.34%), Postives = 153/371 (41.24%), Query Frame = 0


Query: 26  QKRSLERLPSQLADALLRRLHAR--------------RLLYPS--------ILEVFKFTV 85
           Q    ++L ++    +LRRL +R              R  YP         I E FKF +
Sbjct: 42  QSTDRKKLAARAGPHMLRRLASRFTQIVELDLSQSISRSFYPGVTDSDLAVISEGFKF-L 101

Query: 86  EVVDLSGENAVDSEWMAYLG-AYRYLRSLNLSNCHRLSSSGIWAIA-GMTTLRELNLSRC 145
            V++L     +    +A +G     L+ L++S C +LS  G+ A+A G   LR L+L+ C
Sbjct: 102 RVLNLHNCKGITDTGLASIGRCLSLLQFLDVSYCRKLSDKGLSAVAEGCHDLRALHLAGC 161

Query: 146 SKVTDAGIRHLLS-IPTLEKLCIAE-TGITAHGVT-LLSSLKTLVFLDL------GGLAV 205
             +TD  ++ L      LE L +   T IT  G+  L+   + +  LD+      G   V
Sbjct: 162 RFITDESLKSLSERCRDLEALGLQGCTNITDSGLADLVKGCRKIKSLDINKCSNVGDAGV 221

Query: 206 TDQALSSLQVLTKLQYLDLWGSKISNTGSDVLQMFPKLNFLNIAWTNVTKFPNLPHLESL 265
           +  A +    L  L+ LD +  K+ N     L  F K                  +LE+L
Sbjct: 222 SSVAKACASSLKTLKLLDCY--KVGNESISSLAQFCK------------------NLETL 281

Query: 266 NVSNC--VIDSTIKGSGAKVPLRKLIASGATFSNETEDLEFVGTSFLNHLDFSNACLHRF 325
            +  C  + D +I           L+A     S +   +++     LN  D S +C+ + 
Sbjct: 282 IIGGCRDISDESI----------MLLADSCKDSLKNLRMDWC----LNISDSSLSCILKQ 341

Query: 326 CFLPHMKALEHLDL------SSTAIGDNSAGLIASVGENLKHLNLS-CTTVSSSGVGILA 355
           C     K LE LD+      + TA  D  +  +      LK L +S CT ++ +G+G L 
Sbjct: 342 C-----KNLEALDIGCCEEVTDTAFRDLGSDDVL----GLKVLKVSNCTKITVTGIGKLL 368

[Close]

BLAST of MC10g0745 vs. TAIR 10
Match: AT3G58530.1 (RNI-like superfamily protein )

HSP 1 Score: 46.2 bits (108), Expect = 1.0e-04
Identity = 40/134 (29.85%), Postives = 58/134 (43.28%), Query Frame = 0


Query: 88  LRSLNLSNCHRLSSSGIWAIAGMTTLRELNLSRCSKVTDAGIRHLLSIPTLEKLCIAETG 147
           LR L++     +S  GI  IA    L  LNL+ C ++TDAG+                  
Sbjct: 242 LRFLDICGAQNISDEGIGHIAKCNKLESLNLTWCVRITDAGVN----------------- 301

Query: 148 ITAHGVTLLSSLKTLVFLDLGGL-AVTDQALSSLQ-----VLTKLQYLDLWGSKISNTGS 207
                 T+ +S  +L FL L G+  VTD+ L +L       LT L      G K   +  
Sbjct: 302 ------TIANSCTSLEFLSLFGIVGVTDRCLETLSQTCSTTLTTLDVNGCTGIK-RRSRE 351

Query: 208 DVLQMFPKLNFLNI 216
           ++LQMFP+L    +
Sbjct: 362 ELLQMFPRLTCFKV 351

The following BLAST results are available for this feature:

BLAST of MC10g0745 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q17R01	1.2e-05	26.67	F-box/LRR-repeat protein 14 OS=Bos taurus OX=9913 GN=FBXL14 PE=2 SV=1	[more]
Q8N1E6	1.2e-05	26.67	F-box/LRR-repeat protein 14 OS=Homo sapiens OX=9606 GN=FBXL14 PE=1 SV=1	[more]
Q8BID8	1.2e-05	26.67	F-box/LRR-repeat protein 14 OS=Mus musculus OX=10090 GN=Fbxl14 PE=2 SV=1	[more]
Q8NEE6	4.6e-05	27.27	Dynein regulatory complex subunit 6 OS=Homo sapiens OX=9606 GN=FBXL13 PE=2 SV=3	[more]
Q8CDU4	3.0e-04	25.71	Dynein regulatory complex subunit 6 OS=Mus musculus OX=10090 GN=Fbxl13 PE=1 SV=2	[more]

BLAST of MC10g0745 vs. NCBI nr


Match Name	Identity	Description
XP_022136501.1	100.00	F-box/LRR-repeat protein 14 isoform X1 [Momordica charantia]	[more]
KAG6600520.1	86.35	hypothetical protein SDJN03_05753, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022136502.1	100.00	F-box/LRR-repeat protein 14 isoform X2 [Momordica charantia] >XP_022136504.1 F-b...	[more]
XP_022942088.1	86.18	F-box/LRR-repeat protein 14 isoform X1 [Cucurbita moschata] >XP_022942089.1 F-bo...	[more]
XP_023533280.1	86.18	F-box/LRR-repeat protein 14 isoform X1 [Cucurbita pepo subsp. pepo] >XP_02353328...	[more]

BLAST of MC10g0745 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1C3P0	100.00	F-box/LRR-repeat protein 14 isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1C4H3	100.00	F-box/LRR-repeat protein 14 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1FQB0	86.18	F-box/LRR-repeat protein 14 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1IUQ8	85.84	F-box/LRR-repeat protein 14 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114794...	[more]
A0A5A7TU43	83.62	F-box/LRR-repeat protein 14 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of MC10g0745 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23840.1	1.1e-150	50.95	Leucine-rich repeat (LRR) family protein	[more]
AT1G15740.1	1.7e-26	26.65	Leucine-rich repeat family protein	[more]
AT5G27920.1	4.2e-06	23.60	F-box family protein	[more]
AT5G23340.1	2.1e-05	25.34	RNI-like superfamily protein	[more]
AT3G58530.1	1.0e-04	29.85	RNI-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006553	Leucine-rich repeat, cysteine-containing subtype	SMART	SM00367	LRR_CC_2	coord: 430..454 e-value: 350.0 score: 2.5 coord: 478..503 e-value: 200.0 score: 4.4 coord: 111..135 e-value: 0.022 score: 23.9 coord: 321..345 e-value: 90.0 score: 7.1 coord: 85..110 e-value: 5.1 score: 16.1 coord: 346..370 e-value: 520.0 score: 1.1
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 391..510 e-value: 5.8E-10 score: 40.7 coord: 8..147 e-value: 1.3E-19 score: 72.2
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 149..252 e-value: 8.6E-15 score: 56.2
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 253..390 e-value: 3.3E-15 score: 57.8
IPR001611	Leucine-rich repeat	PFAM	PF13516	LRR_6	coord: 86..107 e-value: 1.8 score: 8.8 coord: 111..132 e-value: 0.06 score: 13.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 519..541
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 518..541
None	No IPR available	PANTHER	PTHR48062:SF19	F-BOX/LRR-REPEAT PROTEIN 13	coord: 2..581
None	No IPR available	PANTHER	PTHR48062	RECEPTOR-LIKE PROTEIN 14	coord: 2..581
None	No IPR available	SUPERFAMILY	52058	L domain-like	coord: 62..310
None	No IPR available	SUPERFAMILY	52047	RNI-like	coord: 252..502

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g0745.1	MC10g0745.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031146	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
cellular_component	GO:0019005	SCF ubiquitin ligase complex
molecular_function	GO:0005515	protein binding