MC10g0725 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0725
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptiontranslation initiation factor IF-2
LocationMC10: 6058079 .. 6065051 (-)
RNA-Seq ExpressionMC10g0725
SyntenyMC10g0725
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGAAAATCTGATGCAACTCGAAACCGGTCTAGTTCGACAATAGTTCGGTTCGATGTACGGTCGGTTTTCAGTTCTGACCCAGTAAAACCTGGGCTAAACAAAACCCTACCCTGTCGAATATAGTCTGAACGGTGGGATTCTTCCGAACGACGACGTAGAGTTTCTCTCTCTCCGCCGCAGGTGGCAGCAAGCAGACGCTCTCTCTCTTTCTCTCTCTCGGCAGTCGTCGGCGACCAGCAGAAGGCGGCAATCGGTGGGTATATCGACATTGTTGGCGATCGCTCGTACCTCAAGAAGGTTGAATTCTATTGCTTGCTTGTTTTCTTGGCTTGGTTTTTCAGAAGAGAAAAGAAACCTGAAGAAACAATCTCGATCCAATCTACGAACATTCCATATTTGGCTACTATTGTGCCAAATTTCTGTGCGGAAACGAACCGGTTCGAGCGAATCAAGTCGGTTATATATCAGGGTCGGGTTAATCCATTGTTCTTCGTCTGCCTCTCGATCAATTGGTGCCCTGCTACGCCTTCCTCCATCTCCATCGGAATTCTACACGGCGAGAGCCAACTTTTATTTTCCTCTCCTTTTTCCTTCTGTTTCCCTGCTCTGTAGACTCACCCTTTTGCATTGAAATCTGAGGTTGTGGTTGTGGTTGTGGCAGAGCTCCAGGTACGTCTAATTTTCCAGTTCCTCTGTTCTTTAAGAGGGACCGAAATCTTGGTATCTATTCGAAGCATTCGAAGAACCAAACAACGATTTATTATAAAAATCAGTTACATGCTCGCACAGGTCTTTGACATTTCTACCGAAGTAGTGATACGGTTCGATTCTTGATTATCGACCAACTGAACTGTGTCAGCCCTTCTCGTGTGGTAATGCGAGCATTGTTCTTGGCCACTCTGAAGAGCAGAAAAACATAAGAGGAGATCATCCATAAGTTCGAGGTGAGTTTGCCTTGTTAGTGAGAAAATCAATCGCATTATAATTCCCTAAAACATGTTCATCATCTCGGCATGCTGTACATCTCATAGTCAAGCTATTGTTGAACTCTGCTACCTTTTACGGTGTTCTGCTATCTGGGTGTTGGTTTTTTCTTTTCCCGTAAGTGTCTAATTTTCTTCTTGACTGCTATCTTCCAAATGATGCAGTTTCACCTGGGTCCATTTTAATGTTTAACTATTTTCTCTTTATTGACGGCGAATTTCATATCTGAATGTAGCAATTCTTGTTTTATTTTATCATTTTGTGAATTATGAATCACTCTTTCATATTCACAAATTGGGCTTTTGCATTCCGATTTCTACTTGCTTGGTTTTTAAAATATTTTCTCAATGGTTTTAGGTTTTAGAATAGTTTATCTTCCGGAGAGTTCGTTTGTTTATTTTTTTTGCTTCCTTGATATTTGAGACCAAAGGGCTGCCTTTGGCTTCTGCTTCAAAATTTAAGAATCTTGTTTTTCCTGATATGCAGGTAGCAGTACTCAGATACTTATGAATGGCTTGGAGAGAGCTGGGGAAAAAGGTGAGCTCATGTTCCTAACAATCATGTATCTAACTGAATGTTCTATTTCGTTTTTATTTATTATGAGATCGGAGAATGCGTCCTTAGTTTAAATTTTGTAATGTGTTTAATGACTTCAACCTAGATCACCAATAGCTCAAAAAAAAAAAAAACAAGAAAAAACTTCTTGCATTTTTCAACAGCTAGTCTCAATCATATATTCATTGCTTTACATGTAGTATGGCCTTATCCTTCAATTTATGTTGTGTGAGTTTCATCGGACATAGGGATAATTAAATGCATGTAGGTATTGGCTCCTTCACTTGTTCAAAATTCAAGGGTTCCTCTACCTTTGTTATCTTGTCCCGTGGTCTACTCTGATTCTTCGCCTTTCTATGCGTCGACATGTACAAGTACTGAATTTATAGGGTGTGAGCGGGTTGTATTTGGCCAAATTGGGTAAACCAATGCCCACTCAGCAACACCTTTTTAGCATTTGTAGCCTTGCATCCTCTTTTTGGAACCTCATCCACATCACATTCAATTGGTCCGCAGTTTTACCTACCGACCCCAAAGCTCTCCTACACTTCACTCTTGAGGGACACCCCTTCCGCTAAGAAAAGGAGCTTCTTTGGAAGAATTTTTCAGAGCTTTCTTTTGGACTACTTGACTTGAGAGGAGTCACAGAATGTTTAAAGACAAGGGGCGCACGTTTCCTTCGTTTTTTTATGATATAGTCATGCTAGTTATATCATGGTGTAAATGCTCCCCCTCTTCCATTATTATAGTTTATCGAATCTCTTTTATCCAATTGAAGACTTATTTTGTAACTCCTTTGGATGAGGTCTCTCCTCCTATTTTTGTAATTTCATACCATCAATGAAATTTTTTCTTATAAAAAAAAATAATGGTATATGAAATCAGACTGACTCACAGAACCTTAATCCTTCTAGGTTATTCACAGATTTTGAATTGGTAGAAAATATAGCTGATCTCGTACTATAAGGTGCTTGTTTCTTGTGAAAATTGTTTACCTGAATTACAATTTTTTCTCATTCAGGGAATGCATGCTGGTTTTCGAAGAACACTTACTTGTTCACGAAGGCATTTAGCCAGGCCAAATTTCATTACTACTGATGAGGTTGTTGTTAAGTTAATCCCTGCTTTAGTAAGATGCACTCAAGGTAACACCTTAATTGAAGACATTTTTTGCCTTACTTTATATGGAGCTTGTGAATTTAATTCAATAATCGTGCTTAAGTGACCATTTGAATTTCAAATTTTGGTACAGAATTCCCTTGTGGTTCGACATATCATGGGTCTGATTATTATATGGCTTCTACAGTAGAACCTTGCAGACGGTAACCCAACCATTTTATTACATTACCTTTCCGTTGTTTAGTAACAACACCTACTTGATGCTCCTATTTTTATCCCTTATCAGTTTTGAATAAGTTTCCAATGTAATTCTGCACCGTGAAATATTGACCATTTATAGGGAGAGGATGCTTATATAGATTCATGCATATGCACTTAAAATAAAGATATTTAAACTGGTGGTTTGCAGTGCAACTTATGGTTCTTTTTTTTTTTTGGAAAAAAAAATGTTTAGGTATTTACATTCAAGTACTGAATTACTAGCTAGGAGAGGACATGATCAGGAATTTGGTTTGAAGACTCCAAAGAAAGAAAAGTTTGTAAGAAGGGATGGCCGGAATCAGCCACCAGTTGAAGCTCCATATGTTCCTCCAAAACCAAAGAGCACCGTTGGATCTGTTCCAGATAAAACAATTGAGATATTTGATGGCATGACAATTGTCGAGCTTGCTAAACGTACTGGCAAGTCAATATCCAGACTGCAGGATATTCTCGTAAATGTTGGTGAAAAGATCAACTCAGAGTTTGATCCTCTCAGCATTGACATTGCAGAGCTGGTTGCCATGGTAGGTCTGTTTTAATAGATAATAATTGTATGCTTCGAAATATCCAATTGGCTTAAGCATGTGTCTAGACATCTTGGTCAAAAACTTTTGGCCTTGGAATTAATAAGAATTAGTAACCGGTATTAATATGAATCTCTTTTAATTATATACATTGTTATAAGTGGTCGGTTAATTTTTGTTCTATAAGTGAAGGTCGCTCTCACAATATTATCATGGTGTATGATGTGGGGAAATTGTGTAATTAACAACTATTCTCAGACAGTCTGGCTAATCATCACCTGTTTGTTTTGTGTGGTGTGTGGCTGGGGTGGTAAGTAGGTCCTATTGATTCTGAACGTACTGATACAAATATGATTTTCTGACTCAAATCAATATCATGCAATACAGAAAATAAGATAGACAGAAGACTTAAAATTTCTCAAAAAAAAAAAAAAAATCATAAAAATATTGTAAATTGTAAATCATTTAAGTTTACATATTATAAAGGGTTAAATTATAACACCAAAAGTAGATCTCTTTAATATTCCACGTGAGAACTAAGATAGCAACATATTGTGGTTACTCTTCATTGTCAAGCATCTAATTAAACATGATATATTATGAGTCACCCACAATTATAGTTTATACACCTCGAGTCACCACCAATATAGTCCAAGTTAGTCCATGTTATTAATTTTTTCAGTTTTCTCCATACTGTCATTTTTTGGATATATATAACATGGATGGGTTTTTGAACTATGCACTTGGACTCTTTTGTTTCTTGTGATGAAAAGTTCCTTTCTTTTTTTGGGTGAAAATCTTCATTGCTCGTGTGTTTTTCACCCTTTTCCTTTCCTTTTTTCGGGCTAGGAAGCTTATTCAGTAGTAGTGTTTGGAAGTATATGGTTACAGATTATTTCATCCTTTCAGGAAGTTGGAGTAAACATTAAGAGATTACACTCTAGTGAAGGTTCTCAAATTCAACCACGGCCACCAGTTATTACAGTCATGGGTCATGTTGATCATGGAAAAACTTCTCTTTTAGATGCACTTCGCCAGACATCAGTGGCAGCTAGAGAAGCTGGGGGTATAACTCAGCACCTGGGTGCATTTGTTGTGGGCATGGCTTCAGGTGCTTCAATCACTTTCCTCGACACTCCAGGGCATGCTGCATTTAGTGCTATGCGAGCAAGAGGTGCAGCAGTTACCGATATAGTTGTCCTGGTAGTGGCTGCTGATGATGGGGTGATGCCTCAAACTCTGGAAGCTATGGCACATGCTAAAGCAGCAAACGTGCCCATCGTGCTTGCAATTAATAAATGTGACAAGCCTGCTGCTGATCCCGAGAGAGTCAAACTGCAGCTTGCTTCAGAGGGCTTGCTGCTTGAGGAGATGGGAGGAGATGTTCAAGTTGTTCACGTGTCTGCACTGAAGAAAACAGGATTGGATACCTTGGAGGAAGCACTGCTTCTCCAGGCTGAAATGATGGATTTGAAAGCTCGTATTGACGGACCAGCCCAAGCTTATGTAGTGGAGGCAAGGCTTGACAAGGGTAGAGGTCCTTTGGCTACTACTATAGTGAAGGCAGGGACCTTAGAAAGTGGTCAGTTTGTGGTTGTGGGCTGTGAGTGGGGCAGAATTAGGGCTATTAGGGATATGTTGGGGAATTTGACGGACCGAGCAGGGCCTGCAATGCCTGTTGAGATTGAAGGGTTGAGGGGGCTTCCTATGGCAGGTGATGATATTATTGTTGTAGAGTCTGAGGAGCGTGCACGAATGCTCAGTGCAGGGAGGAAAAGGAAATTTGAGAAGGACAGGTTGAAGAAGCTGAGTGAGGGGAAAACTGAAACTGAAGAGCAATCTGAGGAGGTAATTCAGAGGGTTGAATTACCGATAATAGTAAAAGCTGACGTTCAGGGCACCGTGCAAGCAGTTACAGATGCATTGAAGACTTTAAATAGTCCTCAGGTATACCTCTCTCTCGCTCTCTCATGGTTTTTCAATTTGTGCTCTTTCAATAGCTGTCTGTCTGCAATTATAATAATAACTTTCGTTTCTGGTTCATACGGTAGTTCTTTATTGATCTGTTAAACAATCCCTATCCTTAGCAAAATAATAATAATAATAATAATAAATAAACAATCTCTATCTGGTTTATCCAATGTACTGAACTTTATTATTACTTATTTCTTTATCCATTCATTGTGATAGATAAACTCAATCTAGAATCTTTGATGACCAGTTTGGGTTATTACTTTAAATCACTGCTTATAGTTTGACTTGCAACCTACTTTTAGTCATTAACTTCAATCTTGTCCGTACCGCCCGAAACAATCAATGCTACTTTATAGAATTATTGGAGTGGTTATCTTATACATGGTTGGTTGTCTCATTTTCCAGTCTTCTATGTACTTGAGCAGTTCTCCATAATGTTTCCCATTTCACATCCAGGTTTTTGTAAATGTTGTCCATGTTGGCGTTGGCCCAGTTTCCCAGTCTGATGTGGACTTGGCTCAAGCATGTGGAGCATATATAGTTGGATTTAATGTGAAGAATCCCCCAAGTAATCTCAGTCAGACAGCTACTCAAGCTGGTATAAAGGTATAGGCTAGCTACCTTTCAATCTTACTATCCCCCATGAAATATTGTTCACGTCATCTTCTAAACCAATAAGAAACAATGAGTAGTTGGTTACTGTTGCACATGTTATATGTACATATATATCGAAAAAGAAAGACTGAAAGGCGTACTAAGTTTGGTTTTTCTTCAATGGTATAGATAATTCTACATCGTGTAATCTATCGTCTTTTGGAGGACATTGGGAATCTGATTGTTGACAAGGCACCAGGAACTTCCGAGACGCAGGTTGCTGGGGAGGCCGAGGTGCTGAACATTTTTGAGCTCAAAGGAAGAAGCAAGTCAAAGGGACCCGATGTTAAGATTGCTGGATGTCGGGTGATTGATGGTTGTGTTTCAAGAGCATCGACCATGAGGCTTCTACGAAGCGGGGAAGTTTTGTTCGAAGGATCATGTGCATCTCTCAAGAGGGAGAAACAAGATGTTGATGCCGTGAAGAAAGGAAGCGAGTGTGGACTCGTGATACACGACTGGGATGATTTTCAGGTTGGAGACGTCGTGCAGTGCTTGGAGCAAGTAGTAAGGAAGCCGAAGTTCATTTCATCCGAGAGCGGTGCCGTTAGAATCGAGTGCTGAAGAAAGGGTCAGTAAAATTCATTCTACATGCACTCATTTTAGAGACTTTATATCCATTGGCTGGTCCTATATGATCTGCCACAGTCCAGATATATAGTTTCAAATTTTCTTCAGCATATTTAAGGATAGTAAATTTTGGGAGTGGAAAGAATCTCCATGAGTTGTGACAGATAACCTATGATGTAGAATTGAGGAAGACAATTGATTATTGTACCAACCAAAAGAGAAAAAAACAAATTTTGTTAACTAATAATGTCAATGTGACGACAGGAAAAGCATATTTGAAAACATAGTTATTGTATGTGATGGGTGTTTGTTATTCTGCAACTATGGCTG

mRNA sequence

ATTGAAAATCTGATGCAACTCGAAACCGGTCTAGTTCGACAATAGTTCGGTTCGATGTACGGTCGGTTTTCAGTTCTGACCCAGTAAAACCTGGGCTAAACAAAACCCTACCCTGTCGAATATAGTCTGAACGGTGGGATTCTTCCGAACGACGACGTAGAGTTTCTCTCTCTCCGCCGCAGGTGGCAGCAAGCAGACGCTCTCTCTCTTTCTCTCTCTCGGCAGTCGTCGGCGACCAGCAGAAGGCGGCAATCGGTGGGTATATCGACATTGTTGGCGATCGCTCGTACCTCAAGAAGGTTGAATTCTATTGCTTGCTTGTTTTCTTGGCTTGGTTTTTCAGAAGAGAAAAGAAACCTGAAGAAACAATCTCGATCCAATCTACGAACATTCCATATTTGGCTACTATTGTGCCAAATTTCTGTGCGGAAACGAACCGGTTCGAGCGAATCAAGTCGGTTATATATCAGGGTCGGGTTAATCCATTGTTCTTCGTCTGCCTCTCGATCAATTGGTGCCCTGCTACGCCTTCCTCCATCTCCATCGGAATTCTACACGGCGAGAGCCAACTTTTATTTTCCTCTCCTTTTTCCTTCTGTTTCCCTGCTCTGTAGACTCACCCTTTTGCATTGAAATCTGAGGTTGTGGTTGTGGTTGTGGCAGAGCTCCAGGTCTTTGACATTTCTACCGAAGTAGTGATACGGTTCGATTCTTGATTATCGACCAACTGAACTGTGTCAGCCCTTCTCGTGTGGTAATGCGAGCATTGTTCTTGGCCACTCTGAAGAGCAGAAAAACATAAGAGGAGATCATCCATAAGTTCGAGGTAGCAGTACTCAGATACTTATGAATGGCTTGGAGAGAGCTGGGGAAAAAGGGAATGCATGCTGGTTTTCGAAGAACACTTACTTGTTCACGAAGGCATTTAGCCAGGCCAAATTTCATTACTACTGATGAGGTTGTTGTTAAGTTAATCCCTGCTTTAGTAAGATGCACTCAAGAATTCCCTTGTGGTTCGACATATCATGGGTCTGATTATTATATGGCTTCTACAGTAGAACCTTGCAGACGGTATTTACATTCAAGTACTGAATTACTAGCTAGGAGAGGACATGATCAGGAATTTGGTTTGAAGACTCCAAAGAAAGAAAAGTTTGTAAGAAGGGATGGCCGGAATCAGCCACCAGTTGAAGCTCCATATGTTCCTCCAAAACCAAAGAGCACCGTTGGATCTGTTCCAGATAAAACAATTGAGATATTTGATGGCATGACAATTGTCGAGCTTGCTAAACGTACTGGCAAGTCAATATCCAGACTGCAGGATATTCTCGTAAATGTTGGTGAAAAGATCAACTCAGAGTTTGATCCTCTCAGCATTGACATTGCAGAGCTGGTTGCCATGGAAGTTGGAGTAAACATTAAGAGATTACACTCTAGTGAAGGTTCTCAAATTCAACCACGGCCACCAGTTATTACAGTCATGGGTCATGTTGATCATGGAAAAACTTCTCTTTTAGATGCACTTCGCCAGACATCAGTGGCAGCTAGAGAAGCTGGGGGTATAACTCAGCACCTGGGTGCATTTGTTGTGGGCATGGCTTCAGGTGCTTCAATCACTTTCCTCGACACTCCAGGGCATGCTGCATTTAGTGCTATGCGAGCAAGAGGTGCAGCAGTTACCGATATAGTTGTCCTGGTAGTGGCTGCTGATGATGGGGTGATGCCTCAAACTCTGGAAGCTATGGCACATGCTAAAGCAGCAAACGTGCCCATCGTGCTTGCAATTAATAAATGTGACAAGCCTGCTGCTGATCCCGAGAGAGTCAAACTGCAGCTTGCTTCAGAGGGCTTGCTGCTTGAGGAGATGGGAGGAGATGTTCAAGTTGTTCACGTGTCTGCACTGAAGAAAACAGGATTGGATACCTTGGAGGAAGCACTGCTTCTCCAGGCTGAAATGATGGATTTGAAAGCTCGTATTGACGGACCAGCCCAAGCTTATGTAGTGGAGGCAAGGCTTGACAAGGGTAGAGGTCCTTTGGCTACTACTATAGTGAAGGCAGGGACCTTAGAAAGTGGTCAGTTTGTGGTTGTGGGCTGTGAGTGGGGCAGAATTAGGGCTATTAGGGATATGTTGGGGAATTTGACGGACCGAGCAGGGCCTGCAATGCCTGTTGAGATTGAAGGGTTGAGGGGGCTTCCTATGGCAGGTGATGATATTATTGTTGTAGAGTCTGAGGAGCGTGCACGAATGCTCAGTGCAGGGAGGAAAAGGAAATTTGAGAAGGACAGGTTGAAGAAGCTGAGTGAGGGGAAAACTGAAACTGAAGAGCAATCTGAGGAGGTAATTCAGAGGGTTGAATTACCGATAATAGTAAAAGCTGACGTTCAGGGCACCGTGCAAGCAGTTACAGATGCATTGAAGACTTTAAATAGTCCTCAGGTTTTTGTAAATGTTGTCCATGTTGGCGTTGGCCCAGTTTCCCAGTCTGATGTGGACTTGGCTCAAGCATGTGGAGCATATATAGTTGGATTTAATGTGAAGAATCCCCCAAGTAATCTCAGTCAGACAGCTACTCAAGCTGGTATAAAGATAATTCTACATCGTGTAATCTATCGTCTTTTGGAGGACATTGGGAATCTGATTGTTGACAAGGCACCAGGAACTTCCGAGACGCAGGTTGCTGGGGAGGCCGAGGTGCTGAACATTTTTGAGCTCAAAGGAAGAAGCAAGTCAAAGGGACCCGATGTTAAGATTGCTGGATGTCGGGTGATTGATGGTTGTGTTTCAAGAGCATCGACCATGAGGCTTCTACGAAGCGGGGAAGTTTTGTTCGAAGGATCATGTGCATCTCTCAAGAGGGAGAAACAAGATGTTGATGCCGTGAAGAAAGGAAGCGAGTGTGGACTCGTGATACACGACTGGGATGATTTTCAGGTTGGAGACGTCGTGCAGTGCTTGGAGCAAGTAGTAAGGAAGCCGAAGTTCATTTCATCCGAGAGCGGTGCCGTTAGAATCGAGTGCTGAAGAAAGGGTCAGTAAAATTCATTCTACATGCACTCATTTTAGAGACTTTATATCCATTGGCTGGTCCTATATGATCTGCCACAGTCCAGATATATAGTTTCAAATTTTCTTCAGCATATTTAAGGATAGTAAATTTTGGGAGTGGAAAGAATCTCCATGAGTTGTGACAGATAACCTATGATGTAGAATTGAGGAAGACAATTGATTATTGTACCAACCAAAAGAGAAAAAAACAAATTTTGTTAACTAATAATGTCAATGTGACGACAGGAAAAGCATATTTGAAAACATAGTTATTGTATGTGATGGGTGTTTGTTATTCTGCAACTATGGCTG

Coding sequence (CDS)

ATGGCTTGGAGAGAGCTGGGGAAAAAGGGAATGCATGCTGGTTTTCGAAGAACACTTACTTGTTCACGAAGGCATTTAGCCAGGCCAAATTTCATTACTACTGATGAGGTTGTTGTTAAGTTAATCCCTGCTTTAGTAAGATGCACTCAAGAATTCCCTTGTGGTTCGACATATCATGGGTCTGATTATTATATGGCTTCTACAGTAGAACCTTGCAGACGGTATTTACATTCAAGTACTGAATTACTAGCTAGGAGAGGACATGATCAGGAATTTGGTTTGAAGACTCCAAAGAAAGAAAAGTTTGTAAGAAGGGATGGCCGGAATCAGCCACCAGTTGAAGCTCCATATGTTCCTCCAAAACCAAAGAGCACCGTTGGATCTGTTCCAGATAAAACAATTGAGATATTTGATGGCATGACAATTGTCGAGCTTGCTAAACGTACTGGCAAGTCAATATCCAGACTGCAGGATATTCTCGTAAATGTTGGTGAAAAGATCAACTCAGAGTTTGATCCTCTCAGCATTGACATTGCAGAGCTGGTTGCCATGGAAGTTGGAGTAAACATTAAGAGATTACACTCTAGTGAAGGTTCTCAAATTCAACCACGGCCACCAGTTATTACAGTCATGGGTCATGTTGATCATGGAAAAACTTCTCTTTTAGATGCACTTCGCCAGACATCAGTGGCAGCTAGAGAAGCTGGGGGTATAACTCAGCACCTGGGTGCATTTGTTGTGGGCATGGCTTCAGGTGCTTCAATCACTTTCCTCGACACTCCAGGGCATGCTGCATTTAGTGCTATGCGAGCAAGAGGTGCAGCAGTTACCGATATAGTTGTCCTGGTAGTGGCTGCTGATGATGGGGTGATGCCTCAAACTCTGGAAGCTATGGCACATGCTAAAGCAGCAAACGTGCCCATCGTGCTTGCAATTAATAAATGTGACAAGCCTGCTGCTGATCCCGAGAGAGTCAAACTGCAGCTTGCTTCAGAGGGCTTGCTGCTTGAGGAGATGGGAGGAGATGTTCAAGTTGTTCACGTGTCTGCACTGAAGAAAACAGGATTGGATACCTTGGAGGAAGCACTGCTTCTCCAGGCTGAAATGATGGATTTGAAAGCTCGTATTGACGGACCAGCCCAAGCTTATGTAGTGGAGGCAAGGCTTGACAAGGGTAGAGGTCCTTTGGCTACTACTATAGTGAAGGCAGGGACCTTAGAAAGTGGTCAGTTTGTGGTTGTGGGCTGTGAGTGGGGCAGAATTAGGGCTATTAGGGATATGTTGGGGAATTTGACGGACCGAGCAGGGCCTGCAATGCCTGTTGAGATTGAAGGGTTGAGGGGGCTTCCTATGGCAGGTGATGATATTATTGTTGTAGAGTCTGAGGAGCGTGCACGAATGCTCAGTGCAGGGAGGAAAAGGAAATTTGAGAAGGACAGGTTGAAGAAGCTGAGTGAGGGGAAAACTGAAACTGAAGAGCAATCTGAGGAGGTAATTCAGAGGGTTGAATTACCGATAATAGTAAAAGCTGACGTTCAGGGCACCGTGCAAGCAGTTACAGATGCATTGAAGACTTTAAATAGTCCTCAGGTTTTTGTAAATGTTGTCCATGTTGGCGTTGGCCCAGTTTCCCAGTCTGATGTGGACTTGGCTCAAGCATGTGGAGCATATATAGTTGGATTTAATGTGAAGAATCCCCCAAGTAATCTCAGTCAGACAGCTACTCAAGCTGGTATAAAGATAATTCTACATCGTGTAATCTATCGTCTTTTGGAGGACATTGGGAATCTGATTGTTGACAAGGCACCAGGAACTTCCGAGACGCAGGTTGCTGGGGAGGCCGAGGTGCTGAACATTTTTGAGCTCAAAGGAAGAAGCAAGTCAAAGGGACCCGATGTTAAGATTGCTGGATGTCGGGTGATTGATGGTTGTGTTTCAAGAGCATCGACCATGAGGCTTCTACGAAGCGGGGAAGTTTTGTTCGAAGGATCATGTGCATCTCTCAAGAGGGAGAAACAAGATGTTGATGCCGTGAAGAAAGGAAGCGAGTGTGGACTCGTGATACACGACTGGGATGATTTTCAGGTTGGAGACGTCGTGCAGTGCTTGGAGCAAGTAGTAAGGAAGCCGAAGTTCATTTCATCCGAGAGCGGTGCCGTTAGAATCGAGTGCTGA

Protein sequence

MAWRELGKKGMHAGFRRTLTCSRRHLARPNFITTDEVVVKLIPALVRCTQEFPCGSTYHGSDYYMASTVEPCRRYLHSSTELLARRGHDQEFGLKTPKKEKFVRRDGRNQPPVEAPYVPPKPKSTVGSVPDKTIEIFDGMTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAELVAMEVGVNIKRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQHLGAFVVGMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAHAKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLEEALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGRIRAIRDMLGNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDRLKKLSEGKTETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGVGPVSQSDVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVDKAPGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVLFEGSCASLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLEQVVRKPKFISSESGAVRIEC
Homology
BLAST of MC10g0725 vs. ExPASy Swiss-Prot
Match: A9HF18 (Translation initiation factor IF-2 OS=Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / CCUG 37298 / CIP 103539 / LMG 7603 / PAl5) OX=272568 GN=infB PE=3 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 1.4e-136
Identity = 276/582 (47.42%), Postives = 378/582 (64.95%), Query Frame = 0

Query: 140 MTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAELVAMEVGVNIKRLHSS--- 199
           +T+ ELA R       +   L+ +G  + +    L  D AELV  E G  ++R+  S   
Sbjct: 333 ITVQELANRMAARQGEVIKALMKMG-VMATVTQSLDADTAELVVQEFGHRVRRVADSDVE 392

Query: 200 ---EGSQIQ-----PRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQHLGAFVVG 259
              EG + Q     PRPPV+TVMGHVDHGKTSLLDALR T VAA EAGGITQH+GA+ V 
Sbjct: 393 IGIEGIEDQPEDLLPRPPVVTVMGHVDHGKTSLLDALRTTDVAAAEAGGITQHIGAYQVT 452

Query: 260 MASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAHAKAANVPI 319
           + SG+ ITF+DTPGH AF+AMRARGA+VTD+VVLVVAADDGVMPQT+EA+ HAKAAN PI
Sbjct: 453 LPSGSKITFIDTPGHEAFTAMRARGASVTDVVVLVVAADDGVMPQTIEAIRHAKAANAPI 512

Query: 320 VLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLEEALLLQAE 379
           ++AINKCDKP A+PERV+ +L S  +++E MGGD Q V VSALK+TGLD LEEA+LLQAE
Sbjct: 513 IVAINKCDKPGANPERVRQELLSHEIVVESMGGDTQDVEVSALKRTGLDKLEEAILLQAE 572

Query: 380 MMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGRIRAIRDML 439
           M+DL+A  D  A+  V+E+RLD+GRGP+AT +V+ GTL  G  VV G EWGR+RA+ D  
Sbjct: 573 MLDLRANPDRVAEGSVIESRLDRGRGPVATVLVQKGTLRRGDIVVAGAEWGRVRAMLDDR 632

Query: 440 GNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDRLKKLSEGK 499
           G     A PAMPVEI G+ G+P AG+  +VV++E RAR +S  R+R   +DR    + G+
Sbjct: 633 GRQIQEAAPAMPVEILGIAGVPGAGEPFVVVDNENRAREISEFRQRVI-RDR---TAAGQ 692

Query: 500 TETEEQSEEVIQRV------ELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGVGP 559
           T      ++++ R+      E+ +++KADVQG+ +A+   +  L   +V V V+  GVG 
Sbjct: 693 TAARGTLDQMLARIQAGAQKEVAVLIKADVQGSAEALQATVLKLEHEEVKVRVLTAGVGQ 752

Query: 560 VSQSDVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVDKA 619
           +++SDV LA+A  A I+ FNV+   +   + A + G+ I  + +IY++ +D+  L+  K 
Sbjct: 753 ITESDVQLAKASDAVIIAFNVR-ATTQARELAQREGVDIRYYSIIYQVADDVEQLVKGKV 812

Query: 620 PGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVLFE 679
                 +  G AE+  +F++           K+AGC V +G V R   +RLLR   V+ E
Sbjct: 813 APKHREKFLGYAEIRKVFDITKVG-------KVAGCYVTEGVVKRGCGVRLLRDNVVVHE 872

Query: 680 GSCASLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLE 705
           G  + LKR K DV  V +G ECGL    ++D + GD+V+C E
Sbjct: 873 GELSQLKRFKDDVKEVARGYECGLSFAGYNDLREGDMVECYE 901

BLAST of MC10g0725 vs. ExPASy Swiss-Prot
Match: A7HZ93 (Translation initiation factor IF-2 OS=Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) OX=402881 GN=infB PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 4.0e-136
Identity = 282/592 (47.64%), Postives = 392/592 (66.22%), Query Frame = 0

Query: 132 KTIEIFDGMTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAELVAMEVGVNIK 191
           + + I + +TI ELA R  +    +  IL+  G  +    D +  D A+LVA E+G  +K
Sbjct: 304 REVVIPEVITIQELANRMAERAVDVMKILMKQGIMLKIT-DVIDSDTAQLVAEELGHTVK 363

Query: 192 RLHSSEGSQ-----------IQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQ 251
           R+  S+  +            Q R PV+TVMGHVDHGKTSLLDALR+T VAA EAGGITQ
Sbjct: 364 RVAESDVEEGLVGEADIEEAKQARAPVVTVMGHVDHGKTSLLDALRKTDVAAGEAGGITQ 423

Query: 252 HLGAFVVGMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH 311
           H+GA+ V ++SG  ITFLDTPGHAAF++MRARGA VTDIVVLVVAADDGVMPQT+EA+ H
Sbjct: 424 HIGAYQVNLSSGERITFLDTPGHAAFTSMRARGAKVTDIVVLVVAADDGVMPQTIEAINH 483

Query: 312 AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLE 371
           AKAA VP+++AINK DKP ADP RVK +L    +++E+ GGDV  V +SA    GLD LE
Sbjct: 484 AKAAGVPMIVAINKMDKPEADPTRVKNELLQHEVVVEDFGGDVLTVPISAKTGMGLDKLE 543

Query: 372 EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGR 431
           E +LLQAE++D++A  D  A+  +VEA+LD+GRGP+ T +V+ GTL+ G  +V G EWGR
Sbjct: 544 ETILLQAELLDIRANPDRAAEGIIVEAKLDRGRGPVGTVLVQRGTLKVGDIIVAGAEWGR 603

Query: 432 IRAIRDMLGNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDR 491
           +RA+ +  G   + AGP++PVE+ GL G P AGD I VVESE RAR ++A R+R  ++D+
Sbjct: 604 VRALINDRGENVESAGPSVPVEVLGLGGAPEAGDVISVVESEGRAREVTAYRQR-LQRDK 663

Query: 492 LKKLSEGKTETEEQSEEVIQ--RVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHV 551
            +  + G+T  ++   ++ +  + ELPI+VKADVQG+ +A+  AL+ L + +V   V+HV
Sbjct: 664 -RVGTGGRTSLDQMLSQLKEQDKKELPIVVKADVQGSAEAIVQALEKLGTDEVTARVLHV 723

Query: 552 GVGPVSQSDVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLI 611
           GVG V++SDV LA A  A I+GFNV+   +     A QAG++I  + VIY L++DI   +
Sbjct: 724 GVGGVTESDVTLATASRAPIIGFNVR-ANTQARDAARQAGVEIRYYSVIYDLVDDIKAAM 783

Query: 612 VDKAPGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGE 671
                        G AE+L IF +   SK+     K+AGCRV +G V R S +RL+R   
Sbjct: 784 SGMLSPELRETFLGNAEILEIFNI---SKTG----KVAGCRVTEGVVRRGSHVRLIRDDV 843

Query: 672 VLFEGSCASLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLE-QVVRK 710
           V+ EG  ++LKR K +V  V+ G ECG+    + D + GDV++C + +VV++
Sbjct: 844 VIHEGKLSTLKRFKDEVKEVQSGQECGMAFEGYQDMRKGDVIECFDVEVVQR 884

BLAST of MC10g0725 vs. ExPASy Swiss-Prot
Match: A4XL70 (Translation initiation factor IF-2 OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903 / Tp8T 6331) OX=351627 GN=infB PE=3 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 2.4e-133
Identity = 284/633 (44.87%), Postives = 401/633 (63.35%), Query Frame = 0

Query: 90  QEFGLKTPKKEKFVRRDGRNQP--PVEAPYVPPKPKSTVGSVPDKTIEIFDGMTIVELAK 149
           QE  ++  KKEK  +R    +    ++   V  + K  +  +P+K       +T+ E A 
Sbjct: 242 QEKHVRRGKKEKLKKRSKEQKEVLKLQTKTVQEEKKEEIVKIPEK-------ITVGEFAN 301

Query: 150 RTGKSISRL--QDILVNVGEKINSEFDPLSIDIAELVAMEVGVNIKR----------LHS 209
             GK  + +  + I++ V   IN E D    D+A L+A + G  +++          L  
Sbjct: 302 LIGKPAAEVIKKLIMLGVMANINQEID---FDVASLIAEDYGFKVEKEIIKSEEEILLED 361

Query: 210 SEGSQ--IQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQHLGAFVVGMASGA 269
            E  +  +QPRPPV+ VMGHVDHGKTSLLDA+R T+V  +EAGGITQH+GA VV + +G 
Sbjct: 362 QEDPEETLQPRPPVVVVMGHVDHGKTSLLDAIRNTNVTEKEAGGITQHIGASVVEV-NGR 421

Query: 270 SITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAHAKAANVPIVLAIN 329
            ITFLDTPGH AF+AMRARGA VTDI VLVVAADDGVMPQT+EA+ HAKAANV I++AIN
Sbjct: 422 KITFLDTPGHEAFTAMRARGAQVTDIAVLVVAADDGVMPQTVEAINHAKAANVTIIVAIN 481

Query: 330 KCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLEEALLLQAEMMDLK 389
           K DKP A+PERVK QL+  GL+ EE GGD   V+VSA KK G+D L E +LL A++++LK
Sbjct: 482 KIDKPEANPERVKQQLSEYGLIPEEWGGDTVFVNVSAKKKIGIDHLLEMILLVADLLELK 541

Query: 390 ARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGRIRAIRDMLGNLTD 449
           A  + PA+  V+EA+LDKGRGP+AT +V+ GTL+ G +VVVG  WGR+RA+ D  G    
Sbjct: 542 ANPNRPARGRVIEAKLDKGRGPVATVLVQKGTLKVGDYVVVGSTWGRVRAMIDDKGQRIK 601

Query: 450 RAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDRLKKLSEGKTETEE 509
            AGP+MPVEI GL  +P AGD+++ V+ E+ A+ ++  R+ + +++   K+ + K   +E
Sbjct: 602 EAGPSMPVEILGLEDVPTAGDELVCVKDEKTAKTIAQIRQERLKEE---KMQQSKISLDE 661

Query: 510 QSEEVI--QRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGVGPVSQSDVDLA 569
             E +   Q  EL +I+KADVQG+V+A+  A++ L++ +V V V+H  VG +++SDV LA
Sbjct: 662 LFERIQKGQLKELRVIIKADVQGSVEALKSAIERLSNDKVTVKVIHAAVGAITESDVTLA 721

Query: 570 QACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVDKAPGTSETQVA 629
            A  A I+GFNV+ P       A +  + I ++R+IY ++ DI   +        +  + 
Sbjct: 722 SASDAIIIGFNVR-PEVGAMSLAEKEKVDIRMYRIIYDVINDIKAAMKGLLEPVYKEVII 781

Query: 630 GEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVLFEGSCASLKRE 689
           G AEV  IF    +S S G    IAGC V+DG ++R S  R++R G V++EG  ASLKR 
Sbjct: 782 GHAEVRQIF----KSSSVG---TIAGCYVLDGKITRTSNARIIRDGVVVYEGKLASLKRF 841

Query: 690 KQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLE 705
           K DV  V  G ECG+    ++D + GD+V+  E
Sbjct: 842 KDDVREVAAGYECGMTFEKFNDIKEGDIVEAFE 852

BLAST of MC10g0725 vs. ExPASy Swiss-Prot
Match: B8EIA7 (Translation initiation factor IF-2 OS=Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2) OX=395965 GN=infB PE=3 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 5.4e-133
Identity = 264/584 (45.21%), Postives = 373/584 (63.87%), Query Frame = 0

Query: 140 MTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAELVAMEVGVNIKRLHSSEGS 199
           +TI ELA R  +    +  +++  G+      D +  D A+L+A E+G  +KR+  S+  
Sbjct: 310 ITIQELANRMSERAVDVIKLMMKQGQMAKIT-DVIDADTAQLIAEELGHTVKRVAESDVE 369

Query: 200 Q-----------IQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQHLGAFVVG 259
           +           +  RPPV+T+MGHVDHGKTSLLDALR  +V + EAGGITQH+GA+ + 
Sbjct: 370 EGLFDSPDVEEHLISRPPVVTIMGHVDHGKTSLLDALRHANVVSGEAGGITQHIGAYQIV 429

Query: 260 MASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAHAKAANVPI 319
            ++G  ITF+DTPGHAAF+AMRARGA VTDIVVLVVAADDGVMPQT EA++HAKAA VPI
Sbjct: 430 ASNGLPITFIDTPGHAAFTAMRARGAKVTDIVVLVVAADDGVMPQTAEAISHAKAAGVPI 489

Query: 320 VLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLEEALLLQAE 379
           ++AINK DKP A PERV+ +L    + +E +GGD   V VSA KK  LD L + + LQAE
Sbjct: 490 IVAINKIDKPDAKPERVRQELLQYEVQVESLGGDTLEVEVSATKKINLDKLADLIALQAE 549

Query: 380 MMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGRIRAIRDML 439
           ++DLKA  D PA+  V+EARLDKGRGP+AT +V+ GTL+ G  +V G +WG++RA+ D  
Sbjct: 550 LLDLKASPDRPAEGTVIEARLDKGRGPVATVLVQRGTLKVGDLIVGGSQWGKVRALIDDK 609

Query: 440 GNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDRLKK--LSE 499
           G     AGP+MPVE+ G  G P AGD + VVE+E RAR ++A R R+  +    +  L+ 
Sbjct: 610 GVNRQEAGPSMPVEVLGFSGSPEAGDRVGVVENEARAREIAAYRDRQKREQAAARGNLAR 669

Query: 500 GKTETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGVGPVSQS 559
           G         +   R E P+++KADVQG+++A+   L+ LN+ +V   ++H GVG +++S
Sbjct: 670 GSLADMMSQLKTAARKEFPLVIKADVQGSLEAIVATLEKLNTDEVAARIIHAGVGGITES 729

Query: 560 DVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVDKAPGTS 619
           DV LA+A GA ++GFNV+       Q A Q G++I  + +IY L++D+   +      T 
Sbjct: 730 DVTLAEASGAVLIGFNVRAHKEG-RQLAEQQGLEIRYYNIIYNLVDDVKAAMSGLLAPTL 789

Query: 620 ETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVLFEGSCA 679
              + G AE+L +F +           K+AGCRV DG V R + +RL+R   V+ EG  +
Sbjct: 790 REDMLGNAEILEVFHISKVG-------KVAGCRVTDGRVERGANVRLIRDNVVVHEGKLS 849

Query: 680 SLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQC--LEQVVR 709
           +LKR K +V  V  G ECG+    + D +VGDV++C  +E++ R
Sbjct: 850 TLKRFKDEVKEVVAGQECGMAFEHYQDMRVGDVIECYRVEEIQR 884

BLAST of MC10g0725 vs. ExPASy Swiss-Prot
Match: Q5FQM3 (Translation initiation factor IF-2 OS=Gluconobacter oxydans (strain 621H) OX=290633 GN=infB PE=3 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 7.1e-133
Identity = 265/581 (45.61%), Postives = 367/581 (63.17%), Query Frame = 0

Query: 140 MTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAELVAMEVGVNIKRLHSSE-- 199
           +T+ ELA R       +   L+ +G    +    +  D AELV  E G  IKR+  S+  
Sbjct: 343 ITVAELANRMAARQGEVIKALMKMGVMATAA-QSIDGDTAELVVEEFGHRIKRVSESDVE 402

Query: 200 ---------GSQIQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQHLGAFVVG 259
                       ++PR PV+TVMGHVDHGKTSLLDALR T VAA EAGGITQH+GA+ + 
Sbjct: 403 IGIEGVEDNADDLKPRAPVVTVMGHVDHGKTSLLDALRTTDVAASEAGGITQHIGAYQIT 462

Query: 260 MASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAHAKAANVPI 319
             SG  ITF+DTPGH AF++MRARGA+VTDIVVLVVAADDGVMPQT+EA+ HAKAAN PI
Sbjct: 463 APSGKKITFIDTPGHEAFTSMRARGASVTDIVVLVVAADDGVMPQTIEAIKHAKAANAPI 522

Query: 320 VLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLEEALLLQAE 379
           ++AINK DKP A+P RV+ +L +  +++EEMGGD Q V VSALK+ GLD LEE +LLQ+E
Sbjct: 523 IVAINKIDKPGANPNRVRQELLNHEIVVEEMGGDTQDVEVSALKRIGLDKLEECILLQSE 582

Query: 380 MMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGRIRAIRDML 439
           M+DLKA  D  A+  V+E+RLD+GRGP+A  +V+ GTL  G  VV G EWGR+RA+ D  
Sbjct: 583 MLDLKANPDRVAEGVVIESRLDRGRGPVAALLVQKGTLRRGDIVVAGAEWGRVRAVLDDR 642

Query: 440 GNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDRLKKLSEGK 499
           G     AGP+MPVE+ GL G+P AG+  +VVE++ RAR +S  R+RK ++         +
Sbjct: 643 GRQVKEAGPSMPVEVLGLTGVPGAGEPFVVVENDARAREISEFRQRKIKEKEAASQVAAR 702

Query: 500 TETEEQSEEVIQRV--ELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGVGPVSQS 559
              ++    +   V  E+ +++KADVQG+ +A++  ++ L   +V V V++  VG +++S
Sbjct: 703 GTLDQMLARIQAGVQKEVALLIKADVQGSAEAISTTVQKLAHEEVAVRVLNASVGQITES 762

Query: 560 DVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVDKAPGTS 619
           D+ LA+A  A IV FNV+   +   + A + G+ I  + +IY++ +D+  L+  K     
Sbjct: 763 DIQLAKASDAIIVAFNVR-ATTQARELAQREGVDIRYYSIIYQVADDVEQLVKGKVAPKH 822

Query: 620 ETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVLFEGSCA 679
             +  G AEV  +F +           K+AGC V +G V R   +RLLR   V+ EG  +
Sbjct: 823 REKFLGYAEVRQVFNITKTG-------KVAGCYVTEGLVKRGCGVRLLRDNVVIHEGELS 882

Query: 680 SLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLEQVV 708
            LKR K DV  V +G ECGL    ++D + GD+V+C E  V
Sbjct: 883 QLKRFKDDVKEVARGYECGLSFAGYNDLREGDMVECYEMEV 914

BLAST of MC10g0725 vs. NCBI nr
Match: XP_022136685.1 (uncharacterized protein LOC111008337 [Momordica charantia])

HSP 1 Score: 1395 bits (3612), Expect = 0.0
Identity = 724/724 (100.00%), Postives = 724/724 (100.00%), Query Frame = 0

Query: 1   MAWRELGKKGMHAGFRRTLTCSRRHLARPNFITTDEVVVKLIPALVRCTQEFPCGSTYHG 60
           MAWRELGKKGMHAGFRRTLTCSRRHLARPNFITTDEVVVKLIPALVRCTQEFPCGSTYHG
Sbjct: 1   MAWRELGKKGMHAGFRRTLTCSRRHLARPNFITTDEVVVKLIPALVRCTQEFPCGSTYHG 60

Query: 61  SDYYMASTVEPCRRYLHSSTELLARRGHDQEFGLKTPKKEKFVRRDGRNQPPVEAPYVPP 120
           SDYYMASTVEPCRRYLHSSTELLARRGHDQEFGLKTPKKEKFVRRDGRNQPPVEAPYVPP
Sbjct: 61  SDYYMASTVEPCRRYLHSSTELLARRGHDQEFGLKTPKKEKFVRRDGRNQPPVEAPYVPP 120

Query: 121 KPKSTVGSVPDKTIEIFDGMTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAE 180
           KPKSTVGSVPDKTIEIFDGMTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAE
Sbjct: 121 KPKSTVGSVPDKTIEIFDGMTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAE 180

Query: 181 LVAMEVGVNIKRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQ 240
           LVAMEVGVNIKRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQ
Sbjct: 181 LVAMEVGVNIKRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQ 240

Query: 241 HLGAFVVGMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH 300
           HLGAFVVGMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH
Sbjct: 241 HLGAFVVGMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH 300

Query: 301 AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLE 360
           AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLE
Sbjct: 301 AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLE 360

Query: 361 EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGR 420
           EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGR
Sbjct: 361 EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGR 420

Query: 421 IRAIRDMLGNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDR 480
           IRAIRDMLGNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDR
Sbjct: 421 IRAIRDMLGNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDR 480

Query: 481 LKKLSEGKTETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV 540
           LKKLSEGKTETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV
Sbjct: 481 LKKLSEGKTETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV 540

Query: 541 GPVSQSDVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVD 600
           GPVSQSDVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVD
Sbjct: 541 GPVSQSDVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVD 600

Query: 601 KAPGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVL 660
           KAPGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVL
Sbjct: 601 KAPGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVL 660

Query: 661 FEGSCASLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLEQVVRKPKFISSESGAV 720
           FEGSCASLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLEQVVRKPKFISSESGAV
Sbjct: 661 FEGSCASLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLEQVVRKPKFISSESGAV 720

Query: 721 RIEC 724
           RIEC
Sbjct: 721 RIEC 724

BLAST of MC10g0725 vs. NCBI nr
Match: XP_008451977.1 (PREDICTED: translation initiation factor IF-2 [Cucumis melo])

HSP 1 Score: 1280 bits (3311), Expect = 0.0
Identity = 657/724 (90.75%), Postives = 692/724 (95.58%), Query Frame = 0

Query: 1   MAWRELGKKGMHAGFRRTLTCSRRHLARPNFITTDEVVVKLIPALVRCTQEFPCGSTYHG 60
           MAWRELGKKGMHAG RR+ TCSRRH+ RPNF+TTDEVV+KLIPA  R   + PCGS YHG
Sbjct: 1   MAWRELGKKGMHAGLRRSFTCSRRHIVRPNFVTTDEVVIKLIPASSRFIPDVPCGSAYHG 60

Query: 61  SDYYMASTVEPCRRYLHSSTELLARRGHDQEFGLKTPKKEKFVRRDGRNQPPVEAPYVPP 120
            D+Y+AST+E  RR  HSS ELLA RGHD+EFGLKT K+EKFVRRDG+NQPPVEAPYVPP
Sbjct: 61  PDFYVASTIEAPRRCFHSSAELLAGRGHDKEFGLKTQKREKFVRRDGKNQPPVEAPYVPP 120

Query: 121 KPKSTVGSVPDKTIEIFDGMTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAE 180
           KPK T+GSVPDKTIEIFDGMTIVELAKR+G+SISRLQ+IL NVGEKINSEFDPLSID+AE
Sbjct: 121 KPKHTIGSVPDKTIEIFDGMTIVELAKRSGESISRLQEILTNVGEKINSEFDPLSIDVAE 180

Query: 181 LVAMEVGVNIKRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQ 240
           LVAMEVGVNIKRLHSSEGS+I PRP V+TVMGHVDHGKTSLLDALRQTSVAAREAGGITQ
Sbjct: 181 LVAMEVGVNIKRLHSSEGSEILPRPAVVTVMGHVDHGKTSLLDALRQTSVAAREAGGITQ 240

Query: 241 HLGAFVVGMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH 300
           HLGAFVV MASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH
Sbjct: 241 HLGAFVVEMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH 300

Query: 301 AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLE 360
           AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVV VSALKKTGLD+LE
Sbjct: 301 AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVCVSALKKTGLDSLE 360

Query: 361 EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGR 420
           EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTL +GQ VVVGCEWGR
Sbjct: 361 EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLGTGQLVVVGCEWGR 420

Query: 421 IRAIRDMLGNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDR 480
           IRAIRDM+G L DRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDR
Sbjct: 421 IRAIRDMVGKLADRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDR 480

Query: 481 LKKLSEGKTETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV 540
           LK+LSEGKTETEEQSEEV+QRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV
Sbjct: 481 LKRLSEGKTETEEQSEEVVQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV 540

Query: 541 GPVSQSDVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVD 600
           GPVSQSDVDLAQAC AYIVGFNVKNPPS++SQ+ATQAGIKIILHRVIYRLLED+GNLIVD
Sbjct: 541 GPVSQSDVDLAQACKAYIVGFNVKNPPSSISQSATQAGIKIILHRVIYRLLEDVGNLIVD 600

Query: 601 KAPGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVL 660
           KAPGTSET+VAGE EVLNIFELKGRSKSKGPD+KIAGCRV DGC SR+STMRLLRSGE+L
Sbjct: 601 KAPGTSETRVAGEGEVLNIFELKGRSKSKGPDIKIAGCRVTDGCFSRSSTMRLLRSGEIL 660

Query: 661 FEGSCASLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLEQVVRKPKFISSESGAV 720
           FEGSCASLKREKQDVDAVKKG+ECGLVIH+WDDFQVGD+VQCLEQV+RKPKFISSESGAV
Sbjct: 661 FEGSCASLKREKQDVDAVKKGNECGLVIHNWDDFQVGDIVQCLEQVIRKPKFISSESGAV 720

Query: 721 RIEC 724
           RIEC
Sbjct: 721 RIEC 724

BLAST of MC10g0725 vs. NCBI nr
Match: XP_004149759.1 (uncharacterized protein LOC101205928 [Cucumis sativus] >KGN53381.1 hypothetical protein Csa_015321 [Cucumis sativus])

HSP 1 Score: 1278 bits (3307), Expect = 0.0
Identity = 658/724 (90.88%), Postives = 691/724 (95.44%), Query Frame = 0

Query: 1   MAWRELGKKGMHAGFRRTLTCSRRHLARPNFITTDEVVVKLIPALVRCTQEFPCGSTYHG 60
           MAWRELGKKGMHAG RRT TCSRRH+ RPNF+TTDEVV+KLIPA  R   + PCGS YHG
Sbjct: 1   MAWRELGKKGMHAGLRRTFTCSRRHIVRPNFLTTDEVVIKLIPASSRFIPDVPCGSAYHG 60

Query: 61  SDYYMASTVEPCRRYLHSSTELLARRGHDQEFGLKTPKKEKFVRRDGRNQPPVEAPYVPP 120
           SD+Y+AST+E  RR  HSS ELLA RGHD+EFGLKT KKEKFVR+DGRNQPPVEAPYVPP
Sbjct: 61  SDFYVASTIEAPRRCFHSSAELLAGRGHDKEFGLKTQKKEKFVRKDGRNQPPVEAPYVPP 120

Query: 121 KPKSTVGSVPDKTIEIFDGMTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAE 180
           KPK ++GSV DKTIEIFDGMTIVELAKR+G+SISRLQDIL NVGEKINSEFDPLSID+AE
Sbjct: 121 KPKHSIGSVSDKTIEIFDGMTIVELAKRSGESISRLQDILTNVGEKINSEFDPLSIDVAE 180

Query: 181 LVAMEVGVNIKRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQ 240
           LVAMEVGVNIKRLHSSEGS+I PRP V+TVMGHVDHGKTSLLDALRQTSVAAREAGGITQ
Sbjct: 181 LVAMEVGVNIKRLHSSEGSEILPRPAVVTVMGHVDHGKTSLLDALRQTSVAAREAGGITQ 240

Query: 241 HLGAFVVGMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH 300
           HLGAFVV MASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH
Sbjct: 241 HLGAFVVEMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH 300

Query: 301 AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLE 360
           AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVV+VSALKKTGLD+LE
Sbjct: 301 AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVYVSALKKTGLDSLE 360

Query: 361 EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGR 420
           EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTL SGQFVVVGCEWGR
Sbjct: 361 EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLGSGQFVVVGCEWGR 420

Query: 421 IRAIRDMLGNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDR 480
           IRAIRDM+G L DRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKR+FEKDR
Sbjct: 421 IRAIRDMVGKLADRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRRFEKDR 480

Query: 481 LKKLSEGKTETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV 540
           LKKLSEGKTETEEQSEEV+QRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV
Sbjct: 481 LKKLSEGKTETEEQSEEVVQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV 540

Query: 541 GPVSQSDVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVD 600
           GPVSQSDVDLAQAC AYIVGFNVKNPPS++SQ+ATQAG KII+HRVIY LLED+GNLIVD
Sbjct: 541 GPVSQSDVDLAQACKAYIVGFNVKNPPSSISQSATQAGTKIIIHRVIYHLLEDVGNLIVD 600

Query: 601 KAPGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVL 660
           KAPGTSET+VAGE EVLNIFELKGRSKSKGPD++IAGCRV DGC SR+STMRLLRSGEVL
Sbjct: 601 KAPGTSETRVAGEGEVLNIFELKGRSKSKGPDIRIAGCRVTDGCFSRSSTMRLLRSGEVL 660

Query: 661 FEGSCASLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLEQVVRKPKFISSESGAV 720
           FEGSCASLKREKQDVDAVKKG+ECGLVI +WDDFQVGDVVQCLEQV+RKPKFISSESGAV
Sbjct: 661 FEGSCASLKREKQDVDAVKKGNECGLVIQNWDDFQVGDVVQCLEQVIRKPKFISSESGAV 720

Query: 721 RIEC 724
           RIEC
Sbjct: 721 RIEC 724

BLAST of MC10g0725 vs. NCBI nr
Match: XP_038894926.1 (translation initiation factor IF-2 [Benincasa hispida])

HSP 1 Score: 1269 bits (3285), Expect = 0.0
Identity = 657/725 (90.62%), Postives = 690/725 (95.17%), Query Frame = 0

Query: 1   MAWRELGKKGMHAGFRRTLTCSRRHLARPNFITTDEVVV-KLIPALVRCTQEFPCGSTYH 60
           MAWRELGKKGMHAG RRT TCSRRH+AR +F+TTDEVVV KLIPA  RC  E  CGS YH
Sbjct: 1   MAWRELGKKGMHAGLRRTFTCSRRHIARSSFVTTDEVVVVKLIPASSRCIPEVSCGSAYH 60

Query: 61  GSDYYMASTVEPCRRYLHSSTELLARRGHDQEFGLKTPKKEKFVRRDGRNQPPVEAPYVP 120
           G  +Y+AST+EP RRY HSS ELL RRGH QEFGLKT KKEK VRRD R+QPPVEAPYVP
Sbjct: 61  GPKFYVASTIEPPRRYFHSSAELLVRRGHHQEFGLKTQKKEKSVRRDRRDQPPVEAPYVP 120

Query: 121 PKPKSTVGSVPDKTIEIFDGMTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIA 180
           PKPK T+ SVPDKTIEIFDGMTI ELAKR+G++ISRLQDI++NVGEK++SE+DPLSID+A
Sbjct: 121 PKPKHTIRSVPDKTIEIFDGMTIAELAKRSGETISRLQDIIINVGEKVDSEYDPLSIDVA 180

Query: 181 ELVAMEVGVNIKRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGIT 240
           ELVAMEVGVNIKRLHSSEGS+I PRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGIT
Sbjct: 181 ELVAMEVGVNIKRLHSSEGSEILPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGIT 240

Query: 241 QHLGAFVVGMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMA 300
           QHLGAFVV MASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMA
Sbjct: 241 QHLGAFVVEMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMA 300

Query: 301 HAKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTL 360
           HAKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVV+VSALKKTGLD+L
Sbjct: 301 HAKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVYVSALKKTGLDSL 360

Query: 361 EEALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWG 420
           EEALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWG
Sbjct: 361 EEALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWG 420

Query: 421 RIRAIRDMLGNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKD 480
           RIR IRDM+G L DRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKD
Sbjct: 421 RIRVIRDMVGKLADRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKD 480

Query: 481 RLKKLSEGKTETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVG 540
           RLKKLSEGKTETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALK L  PQVF+NVVHVG
Sbjct: 481 RLKKLSEGKTETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKPLTVPQVFLNVVHVG 540

Query: 541 VGPVSQSDVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIV 600
           VGPVSQSDVDLAQACGAYIVGFNVKNPPS++SQ+ATQAGIKIILHRVIY LLEDIGNLIV
Sbjct: 541 VGPVSQSDVDLAQACGAYIVGFNVKNPPSSISQSATQAGIKIILHRVIYHLLEDIGNLIV 600

Query: 601 DKAPGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEV 660
           DKAPGTSETQ+AGEAEVLNIFELKGRSKSKGPDVKIAGCRV+DG  SR+STMRLLRSGEV
Sbjct: 601 DKAPGTSETQIAGEAEVLNIFELKGRSKSKGPDVKIAGCRVLDGSFSRSSTMRLLRSGEV 660

Query: 661 LFEGSCASLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLEQVVRKPKFISSESGA 720
           LFEGSCASLKREKQDVD+VKKG+ECGLVIH+W+DFQ+GDVVQCLEQVVRKPKFISSESGA
Sbjct: 661 LFEGSCASLKREKQDVDSVKKGNECGLVIHNWNDFQIGDVVQCLEQVVRKPKFISSESGA 720

Query: 721 VRIEC 724
           VRIEC
Sbjct: 721 VRIEC 725

BLAST of MC10g0725 vs. NCBI nr
Match: KAA0044874.1 (translation initiation factor IF-2 [Cucumis melo var. makuwa])

HSP 1 Score: 1260 bits (3260), Expect = 0.0
Identity = 649/714 (90.90%), Postives = 682/714 (95.52%), Query Frame = 0

Query: 11  MHAGFRRTLTCSRRHLARPNFITTDEVVVKLIPALVRCTQEFPCGSTYHGSDYYMASTVE 70
           MHAG RR+ TCSRRH+ RPNF+TTDEVV+KLIPA  R   + PCGS YHG D+Y+AST+E
Sbjct: 1   MHAGLRRSFTCSRRHIVRPNFVTTDEVVIKLIPASSRFIPDVPCGSAYHGPDFYVASTIE 60

Query: 71  PCRRYLHSSTELLARRGHDQEFGLKTPKKEKFVRRDGRNQPPVEAPYVPPKPKSTVGSVP 130
             RR  HSS ELLA RGHD+EFGLKT K+EKFVRRDG+NQPPVEAPYVPPKPK T+GSVP
Sbjct: 61  APRRCFHSSAELLAGRGHDKEFGLKTQKREKFVRRDGKNQPPVEAPYVPPKPKHTIGSVP 120

Query: 131 DKTIEIFDGMTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAELVAMEVGVNI 190
           DKTIEIFDGMTIVELAKR+G+SISRLQ+IL NVGEKINSEFDPLSID+AELVAMEVGVNI
Sbjct: 121 DKTIEIFDGMTIVELAKRSGESISRLQEILTNVGEKINSEFDPLSIDVAELVAMEVGVNI 180

Query: 191 KRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQHLGAFVVGMA 250
           KRLHSSEGS+I PRP V+TVMGHVDHGKTSLLDALRQTSVAAREAGGITQHLGAFVV MA
Sbjct: 181 KRLHSSEGSEILPRPAVVTVMGHVDHGKTSLLDALRQTSVAAREAGGITQHLGAFVVEMA 240

Query: 251 SGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAHAKAANVPIVL 310
           SGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAHAKAANVPIVL
Sbjct: 241 SGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAHAKAANVPIVL 300

Query: 311 AINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLEEALLLQAEMM 370
           AINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVV VSALKKTGLD+LEEALLLQAEMM
Sbjct: 301 AINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVCVSALKKTGLDSLEEALLLQAEMM 360

Query: 371 DLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGRIRAIRDMLGN 430
           DLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTL +GQ VVVGCEWGRIRAIRDM+G 
Sbjct: 361 DLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLGTGQLVVVGCEWGRIRAIRDMVGK 420

Query: 431 LTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDRLKKLSEGKTE 490
           L DRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDRLKKLSEGKTE
Sbjct: 421 LADRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDRLKKLSEGKTE 480

Query: 491 TEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGVGPVSQSDVDL 550
           TEEQSEEV+QRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGVGPVSQSDVDL
Sbjct: 481 TEEQSEEVVQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGVGPVSQSDVDL 540

Query: 551 AQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVDKAPGTSETQV 610
           AQAC AYIVGFNVKNPPS++SQ+ATQAGIKIILHRVIYRLLED+GNLIVDKAPGTSET+V
Sbjct: 541 AQACKAYIVGFNVKNPPSSISQSATQAGIKIILHRVIYRLLEDVGNLIVDKAPGTSETRV 600

Query: 611 AGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVLFEGSCASLKR 670
           AGE EVLNIFELKGRSKSKGPD+KIAGCRV DGC SR+STMRLLRSGEVLFEGSCASLKR
Sbjct: 601 AGEGEVLNIFELKGRSKSKGPDIKIAGCRVTDGCFSRSSTMRLLRSGEVLFEGSCASLKR 660

Query: 671 EKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLEQVVRKPKFISSESGAVRIEC 724
           EKQDVDAVKKG+ECGLVIH+WDDFQVGD+VQCLEQV+RKPKFISSESGAVRIEC
Sbjct: 661 EKQDVDAVKKGNECGLVIHNWDDFQVGDIVQCLEQVIRKPKFISSESGAVRIEC 714

BLAST of MC10g0725 vs. ExPASy TrEMBL
Match: A0A6J1C4N0 (uncharacterized protein LOC111008337 OS=Momordica charantia OX=3673 GN=LOC111008337 PE=3 SV=1)

HSP 1 Score: 1395 bits (3612), Expect = 0.0
Identity = 724/724 (100.00%), Postives = 724/724 (100.00%), Query Frame = 0

Query: 1   MAWRELGKKGMHAGFRRTLTCSRRHLARPNFITTDEVVVKLIPALVRCTQEFPCGSTYHG 60
           MAWRELGKKGMHAGFRRTLTCSRRHLARPNFITTDEVVVKLIPALVRCTQEFPCGSTYHG
Sbjct: 1   MAWRELGKKGMHAGFRRTLTCSRRHLARPNFITTDEVVVKLIPALVRCTQEFPCGSTYHG 60

Query: 61  SDYYMASTVEPCRRYLHSSTELLARRGHDQEFGLKTPKKEKFVRRDGRNQPPVEAPYVPP 120
           SDYYMASTVEPCRRYLHSSTELLARRGHDQEFGLKTPKKEKFVRRDGRNQPPVEAPYVPP
Sbjct: 61  SDYYMASTVEPCRRYLHSSTELLARRGHDQEFGLKTPKKEKFVRRDGRNQPPVEAPYVPP 120

Query: 121 KPKSTVGSVPDKTIEIFDGMTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAE 180
           KPKSTVGSVPDKTIEIFDGMTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAE
Sbjct: 121 KPKSTVGSVPDKTIEIFDGMTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAE 180

Query: 181 LVAMEVGVNIKRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQ 240
           LVAMEVGVNIKRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQ
Sbjct: 181 LVAMEVGVNIKRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQ 240

Query: 241 HLGAFVVGMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH 300
           HLGAFVVGMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH
Sbjct: 241 HLGAFVVGMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH 300

Query: 301 AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLE 360
           AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLE
Sbjct: 301 AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLE 360

Query: 361 EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGR 420
           EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGR
Sbjct: 361 EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGR 420

Query: 421 IRAIRDMLGNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDR 480
           IRAIRDMLGNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDR
Sbjct: 421 IRAIRDMLGNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDR 480

Query: 481 LKKLSEGKTETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV 540
           LKKLSEGKTETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV
Sbjct: 481 LKKLSEGKTETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV 540

Query: 541 GPVSQSDVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVD 600
           GPVSQSDVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVD
Sbjct: 541 GPVSQSDVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVD 600

Query: 601 KAPGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVL 660
           KAPGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVL
Sbjct: 601 KAPGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVL 660

Query: 661 FEGSCASLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLEQVVRKPKFISSESGAV 720
           FEGSCASLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLEQVVRKPKFISSESGAV
Sbjct: 661 FEGSCASLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLEQVVRKPKFISSESGAV 720

Query: 721 RIEC 724
           RIEC
Sbjct: 721 RIEC 724

BLAST of MC10g0725 vs. ExPASy TrEMBL
Match: A0A1S3BS61 (translation initiation factor IF-2 OS=Cucumis melo OX=3656 GN=LOC103493119 PE=3 SV=1)

HSP 1 Score: 1280 bits (3311), Expect = 0.0
Identity = 657/724 (90.75%), Postives = 692/724 (95.58%), Query Frame = 0

Query: 1   MAWRELGKKGMHAGFRRTLTCSRRHLARPNFITTDEVVVKLIPALVRCTQEFPCGSTYHG 60
           MAWRELGKKGMHAG RR+ TCSRRH+ RPNF+TTDEVV+KLIPA  R   + PCGS YHG
Sbjct: 1   MAWRELGKKGMHAGLRRSFTCSRRHIVRPNFVTTDEVVIKLIPASSRFIPDVPCGSAYHG 60

Query: 61  SDYYMASTVEPCRRYLHSSTELLARRGHDQEFGLKTPKKEKFVRRDGRNQPPVEAPYVPP 120
            D+Y+AST+E  RR  HSS ELLA RGHD+EFGLKT K+EKFVRRDG+NQPPVEAPYVPP
Sbjct: 61  PDFYVASTIEAPRRCFHSSAELLAGRGHDKEFGLKTQKREKFVRRDGKNQPPVEAPYVPP 120

Query: 121 KPKSTVGSVPDKTIEIFDGMTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAE 180
           KPK T+GSVPDKTIEIFDGMTIVELAKR+G+SISRLQ+IL NVGEKINSEFDPLSID+AE
Sbjct: 121 KPKHTIGSVPDKTIEIFDGMTIVELAKRSGESISRLQEILTNVGEKINSEFDPLSIDVAE 180

Query: 181 LVAMEVGVNIKRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQ 240
           LVAMEVGVNIKRLHSSEGS+I PRP V+TVMGHVDHGKTSLLDALRQTSVAAREAGGITQ
Sbjct: 181 LVAMEVGVNIKRLHSSEGSEILPRPAVVTVMGHVDHGKTSLLDALRQTSVAAREAGGITQ 240

Query: 241 HLGAFVVGMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH 300
           HLGAFVV MASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH
Sbjct: 241 HLGAFVVEMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH 300

Query: 301 AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLE 360
           AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVV VSALKKTGLD+LE
Sbjct: 301 AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVCVSALKKTGLDSLE 360

Query: 361 EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGR 420
           EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTL +GQ VVVGCEWGR
Sbjct: 361 EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLGTGQLVVVGCEWGR 420

Query: 421 IRAIRDMLGNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDR 480
           IRAIRDM+G L DRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDR
Sbjct: 421 IRAIRDMVGKLADRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDR 480

Query: 481 LKKLSEGKTETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV 540
           LK+LSEGKTETEEQSEEV+QRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV
Sbjct: 481 LKRLSEGKTETEEQSEEVVQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV 540

Query: 541 GPVSQSDVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVD 600
           GPVSQSDVDLAQAC AYIVGFNVKNPPS++SQ+ATQAGIKIILHRVIYRLLED+GNLIVD
Sbjct: 541 GPVSQSDVDLAQACKAYIVGFNVKNPPSSISQSATQAGIKIILHRVIYRLLEDVGNLIVD 600

Query: 601 KAPGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVL 660
           KAPGTSET+VAGE EVLNIFELKGRSKSKGPD+KIAGCRV DGC SR+STMRLLRSGE+L
Sbjct: 601 KAPGTSETRVAGEGEVLNIFELKGRSKSKGPDIKIAGCRVTDGCFSRSSTMRLLRSGEIL 660

Query: 661 FEGSCASLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLEQVVRKPKFISSESGAV 720
           FEGSCASLKREKQDVDAVKKG+ECGLVIH+WDDFQVGD+VQCLEQV+RKPKFISSESGAV
Sbjct: 661 FEGSCASLKREKQDVDAVKKGNECGLVIHNWDDFQVGDIVQCLEQVIRKPKFISSESGAV 720

Query: 721 RIEC 724
           RIEC
Sbjct: 721 RIEC 724

BLAST of MC10g0725 vs. ExPASy TrEMBL
Match: A0A0A0KZS3 (Tr-type G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G050840 PE=3 SV=1)

HSP 1 Score: 1278 bits (3307), Expect = 0.0
Identity = 658/724 (90.88%), Postives = 691/724 (95.44%), Query Frame = 0

Query: 1   MAWRELGKKGMHAGFRRTLTCSRRHLARPNFITTDEVVVKLIPALVRCTQEFPCGSTYHG 60
           MAWRELGKKGMHAG RRT TCSRRH+ RPNF+TTDEVV+KLIPA  R   + PCGS YHG
Sbjct: 1   MAWRELGKKGMHAGLRRTFTCSRRHIVRPNFLTTDEVVIKLIPASSRFIPDVPCGSAYHG 60

Query: 61  SDYYMASTVEPCRRYLHSSTELLARRGHDQEFGLKTPKKEKFVRRDGRNQPPVEAPYVPP 120
           SD+Y+AST+E  RR  HSS ELLA RGHD+EFGLKT KKEKFVR+DGRNQPPVEAPYVPP
Sbjct: 61  SDFYVASTIEAPRRCFHSSAELLAGRGHDKEFGLKTQKKEKFVRKDGRNQPPVEAPYVPP 120

Query: 121 KPKSTVGSVPDKTIEIFDGMTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAE 180
           KPK ++GSV DKTIEIFDGMTIVELAKR+G+SISRLQDIL NVGEKINSEFDPLSID+AE
Sbjct: 121 KPKHSIGSVSDKTIEIFDGMTIVELAKRSGESISRLQDILTNVGEKINSEFDPLSIDVAE 180

Query: 181 LVAMEVGVNIKRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQ 240
           LVAMEVGVNIKRLHSSEGS+I PRP V+TVMGHVDHGKTSLLDALRQTSVAAREAGGITQ
Sbjct: 181 LVAMEVGVNIKRLHSSEGSEILPRPAVVTVMGHVDHGKTSLLDALRQTSVAAREAGGITQ 240

Query: 241 HLGAFVVGMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH 300
           HLGAFVV MASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH
Sbjct: 241 HLGAFVVEMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAH 300

Query: 301 AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLE 360
           AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVV+VSALKKTGLD+LE
Sbjct: 301 AKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVYVSALKKTGLDSLE 360

Query: 361 EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGR 420
           EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTL SGQFVVVGCEWGR
Sbjct: 361 EALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLGSGQFVVVGCEWGR 420

Query: 421 IRAIRDMLGNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDR 480
           IRAIRDM+G L DRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKR+FEKDR
Sbjct: 421 IRAIRDMVGKLADRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRRFEKDR 480

Query: 481 LKKLSEGKTETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV 540
           LKKLSEGKTETEEQSEEV+QRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV
Sbjct: 481 LKKLSEGKTETEEQSEEVVQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGV 540

Query: 541 GPVSQSDVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVD 600
           GPVSQSDVDLAQAC AYIVGFNVKNPPS++SQ+ATQAG KII+HRVIY LLED+GNLIVD
Sbjct: 541 GPVSQSDVDLAQACKAYIVGFNVKNPPSSISQSATQAGTKIIIHRVIYHLLEDVGNLIVD 600

Query: 601 KAPGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVL 660
           KAPGTSET+VAGE EVLNIFELKGRSKSKGPD++IAGCRV DGC SR+STMRLLRSGEVL
Sbjct: 601 KAPGTSETRVAGEGEVLNIFELKGRSKSKGPDIRIAGCRVTDGCFSRSSTMRLLRSGEVL 660

Query: 661 FEGSCASLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLEQVVRKPKFISSESGAV 720
           FEGSCASLKREKQDVDAVKKG+ECGLVI +WDDFQVGDVVQCLEQV+RKPKFISSESGAV
Sbjct: 661 FEGSCASLKREKQDVDAVKKGNECGLVIQNWDDFQVGDVVQCLEQVIRKPKFISSESGAV 720

Query: 721 RIEC 724
           RIEC
Sbjct: 721 RIEC 724

BLAST of MC10g0725 vs. ExPASy TrEMBL
Match: A0A5A7TP11 (Translation initiation factor IF-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G001670 PE=3 SV=1)

HSP 1 Score: 1260 bits (3260), Expect = 0.0
Identity = 649/714 (90.90%), Postives = 682/714 (95.52%), Query Frame = 0

Query: 11  MHAGFRRTLTCSRRHLARPNFITTDEVVVKLIPALVRCTQEFPCGSTYHGSDYYMASTVE 70
           MHAG RR+ TCSRRH+ RPNF+TTDEVV+KLIPA  R   + PCGS YHG D+Y+AST+E
Sbjct: 1   MHAGLRRSFTCSRRHIVRPNFVTTDEVVIKLIPASSRFIPDVPCGSAYHGPDFYVASTIE 60

Query: 71  PCRRYLHSSTELLARRGHDQEFGLKTPKKEKFVRRDGRNQPPVEAPYVPPKPKSTVGSVP 130
             RR  HSS ELLA RGHD+EFGLKT K+EKFVRRDG+NQPPVEAPYVPPKPK T+GSVP
Sbjct: 61  APRRCFHSSAELLAGRGHDKEFGLKTQKREKFVRRDGKNQPPVEAPYVPPKPKHTIGSVP 120

Query: 131 DKTIEIFDGMTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAELVAMEVGVNI 190
           DKTIEIFDGMTIVELAKR+G+SISRLQ+IL NVGEKINSEFDPLSID+AELVAMEVGVNI
Sbjct: 121 DKTIEIFDGMTIVELAKRSGESISRLQEILTNVGEKINSEFDPLSIDVAELVAMEVGVNI 180

Query: 191 KRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQHLGAFVVGMA 250
           KRLHSSEGS+I PRP V+TVMGHVDHGKTSLLDALRQTSVAAREAGGITQHLGAFVV MA
Sbjct: 181 KRLHSSEGSEILPRPAVVTVMGHVDHGKTSLLDALRQTSVAAREAGGITQHLGAFVVEMA 240

Query: 251 SGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAHAKAANVPIVL 310
           SGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAHAKAANVPIVL
Sbjct: 241 SGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAHAKAANVPIVL 300

Query: 311 AINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVHVSALKKTGLDTLEEALLLQAEMM 370
           AINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVV VSALKKTGLD+LEEALLLQAEMM
Sbjct: 301 AINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVVCVSALKKTGLDSLEEALLLQAEMM 360

Query: 371 DLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESGQFVVVGCEWGRIRAIRDMLGN 430
           DLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTL +GQ VVVGCEWGRIRAIRDM+G 
Sbjct: 361 DLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLGTGQLVVVGCEWGRIRAIRDMVGK 420

Query: 431 LTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDRLKKLSEGKTE 490
           L DRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDRLKKLSEGKTE
Sbjct: 421 LADRAGPAMPVEIEGLRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDRLKKLSEGKTE 480

Query: 491 TEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGVGPVSQSDVDL 550
           TEEQSEEV+QRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGVGPVSQSDVDL
Sbjct: 481 TEEQSEEVVQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGVGPVSQSDVDL 540

Query: 551 AQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVDKAPGTSETQV 610
           AQAC AYIVGFNVKNPPS++SQ+ATQAGIKIILHRVIYRLLED+GNLIVDKAPGTSET+V
Sbjct: 541 AQACKAYIVGFNVKNPPSSISQSATQAGIKIILHRVIYRLLEDVGNLIVDKAPGTSETRV 600

Query: 611 AGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVLFEGSCASLKR 670
           AGE EVLNIFELKGRSKSKGPD+KIAGCRV DGC SR+STMRLLRSGEVLFEGSCASLKR
Sbjct: 601 AGEGEVLNIFELKGRSKSKGPDIKIAGCRVTDGCFSRSSTMRLLRSGEVLFEGSCASLKR 660

Query: 671 EKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLEQVVRKPKFISSESGAVRIEC 724
           EKQDVDAVKKG+ECGLVIH+WDDFQVGD+VQCLEQV+RKPKFISSESGAVRIEC
Sbjct: 661 EKQDVDAVKKGNECGLVIHNWDDFQVGDIVQCLEQVIRKPKFISSESGAVRIEC 714

BLAST of MC10g0725 vs. ExPASy TrEMBL
Match: A0A5D3D1N2 (Translation initiation factor IF-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004190 PE=3 SV=1)

HSP 1 Score: 1245 bits (3221), Expect = 0.0
Identity = 647/738 (87.67%), Postives = 682/738 (92.41%), Query Frame = 0

Query: 11  MHAGFRRTLTCSRRHLARPNFITTDEVVVKLIPALVRCTQEFPCGSTYHGSDYYMASTVE 70
           MHAG RR+ TCSRRH+ RPNF+TTDEVV+KLIPA  R   + PCGS YHG D+Y+AST+E
Sbjct: 1   MHAGLRRSFTCSRRHIVRPNFVTTDEVVIKLIPASSRFIPDVPCGSAYHGPDFYVASTIE 60

Query: 71  PCRRYLHSSTELLARRGHDQEFGLKTPKKEKFVRRDGRNQPPVEAPYVPPKPKSTVGSVP 130
             RR  HSS ELLA RGHD+EFGLKT K+EKFVRRDG+NQPPVEAPYVPPKPK T+GSVP
Sbjct: 61  APRRCFHSSAELLAGRGHDKEFGLKTQKREKFVRRDGKNQPPVEAPYVPPKPKHTIGSVP 120

Query: 131 DKTIEIFDGMTIVELAKRTGKSISRLQDILVNVGEKINSEFDPLSIDIAELVAM------ 190
           DKTIEIFDGMTIVELAKR+G+SISRLQ+IL NVGEKINSEFDPLSID+AELVAM      
Sbjct: 121 DKTIEIFDGMTIVELAKRSGESISRLQEILTNVGEKINSEFDPLSIDVAELVAMTFDRGL 180

Query: 191 ------------------EVGVNIKRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALR 250
                             EVGVNIKRLHSSEGS+I PRP V+TVMGHVDHGKTSLLDALR
Sbjct: 181 CPLLCTISSPLVDYFEEKEVGVNIKRLHSSEGSEILPRPAVVTVMGHVDHGKTSLLDALR 240

Query: 251 QTSVAAREAGGITQHLGAFVVGMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAA 310
           QTSVAAREAGGITQHLGAFVV MASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAA
Sbjct: 241 QTSVAAREAGGITQHLGAFVVEMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAA 300

Query: 311 DDGVMPQTLEAMAHAKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVV 370
           DDGVMPQTLEAMAHAKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVV
Sbjct: 301 DDGVMPQTLEAMAHAKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVV 360

Query: 371 HVSALKKTGLDTLEEALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTL 430
            VSALKKTGLD+LEEALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTL
Sbjct: 361 CVSALKKTGLDSLEEALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTL 420

Query: 431 ESGQFVVVGCEWGRIRAIRDMLGNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERAR 490
            +GQ VVVGCEWGRIRAIRDM+G L DRAGPAMPVEIEGLRGLPMAGDDIIVVESEERAR
Sbjct: 421 GTGQLVVVGCEWGRIRAIRDMVGKLADRAGPAMPVEIEGLRGLPMAGDDIIVVESEERAR 480

Query: 491 MLSAGRKRKFEKDRLKKLSEGKTETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTL 550
           MLSAGRKRKFEKDRLK+LSEGKTETEEQSEEV+QRVELPIIVKADVQGTVQAVTDALKTL
Sbjct: 481 MLSAGRKRKFEKDRLKRLSEGKTETEEQSEEVVQRVELPIIVKADVQGTVQAVTDALKTL 540

Query: 551 NSPQVFVNVVHVGVGPVSQSDVDLAQACGAYIVGFNVKNPPSNLSQTATQAGIKIILHRV 610
           NSPQVFVNVVHVGVGPVSQSDVDLAQAC AYIVGFNVKNPPS++SQ+ATQAGIKIILHRV
Sbjct: 541 NSPQVFVNVVHVGVGPVSQSDVDLAQACKAYIVGFNVKNPPSSISQSATQAGIKIILHRV 600

Query: 611 IYRLLEDIGNLIVDKAPGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVS 670
           IYRLLED+GNLIVDKAPGTSET+VAGE EVLNIFELKGRSKSKGPD+KIAGCRV DGC S
Sbjct: 601 IYRLLEDVGNLIVDKAPGTSETRVAGEGEVLNIFELKGRSKSKGPDIKIAGCRVTDGCFS 660

Query: 671 RASTMRLLRSGEVLFEGSCASLKREKQDVDAVKKGSECGLVIHDWDDFQVGDVVQCLEQV 724
           R+STMRLLRSGE+LFEGSCASLKREKQDVDAVKKG+ECGLVIH+WDDFQVGD+VQCLEQV
Sbjct: 661 RSSTMRLLRSGEILFEGSCASLKREKQDVDAVKKGNECGLVIHNWDDFQVGDIVQCLEQV 720

BLAST of MC10g0725 vs. TAIR 10
Match: AT4G11160.1 (Translation initiation factor 2, small GTP-binding protein )

HSP 1 Score: 902.1 bits (2330), Expect = 2.8e-262
Identity = 477/689 (69.23%), Postives = 563/689 (81.71%), Query Frame = 0

Query: 52  FPCGSTYHGSDYYMASTVEPCRRYLHSSTELLARRGHD-------QEFGLKTPK-KEKFV 111
           F   S  + S  Y +   E   RY H+S E LA+R  D       +E   +T K K KF 
Sbjct: 57  FGFSSLSNWSRSYESFPTETSIRYFHASRETLAKRKEDADRLLSHRERKKQTVKTKGKFS 116

Query: 112 RRDGR-NQPPVEAPYVPPKPKSTVGSVPDKTIEIFDGMTIVELAKRTGKSISRLQDILVN 171
           +R+ + ++PPVEAPYVPP+ K     +P KT++IF+GMT++EL+KRTG+S++ LQ IL+N
Sbjct: 117 KREKKTDKPPVEAPYVPPRLKRLAKGLPGKTVDIFEGMTLLELSKRTGESVAVLQSILIN 176

Query: 172 VGEKINSEFDPLSIDIAELVAMEVGVNIKRLHSSEGSQIQPRPPVITVMGHVDHGKTSLL 231
           VGE  +SEFD +S+D+AEL+AME+G+N++R HS+EGS+I PRPPV+TVMGHVDHGKTSLL
Sbjct: 177 VGETFSSEFDTISVDVAELLAMEIGINVRRQHSTEGSEILPRPPVVTVMGHVDHGKTSLL 236

Query: 232 DALRQTSVAAREAGGITQHLGAFVVGMA-SGASITFLDTPGHAAFSAMRARGAAVTDIVV 291
           DALR TSVAAREAGGITQH+GAFVVGM  SG SITFLDTPGHAAFS MRARGAAVTDIVV
Sbjct: 237 DALRNTSVAAREAGGITQHVGAFVVGMPDSGTSITFLDTPGHAAFSEMRARGAAVTDIVV 296

Query: 292 LVVAADDGVMPQTLEAMAHAKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGG 351
           LVVAADDGVMPQTLEA+AHA++ANVP+V+AINKCDKP A+PE+VK QL SEG+ LE++GG
Sbjct: 297 LVVAADDGVMPQTLEAIAHARSANVPVVVAINKCDKPGANPEKVKYQLTSEGIELEDIGG 356

Query: 352 DVQVVHVSALKKTGLDTLEEALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIV 411
           +VQ V VSA K TGLD LEEALLLQA  MDLKAR+DGPAQAYVVEARLDKGRGPLAT IV
Sbjct: 357 NVQAVEVSAAKSTGLDKLEEALLLQAVDMDLKARVDGPAQAYVVEARLDKGRGPLATIIV 416

Query: 412 KAGTLESGQFVVVGCEWGRIRAIRDMLGNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVES 471
           KAGTL  GQ VV+GC+WGR+RAIRDM+G  TDRA PAMPVEIEGL+GLPMAGDD+IVVES
Sbjct: 417 KAGTLVRGQHVVIGCQWGRLRAIRDMIGKTTDRATPAMPVEIEGLKGLPMAGDDVIVVES 476

Query: 472 EERARMLSAGRKRKFEKDRLKKLSEGKTE-----TEEQSEEVIQRVELPIIVKADVQGTV 531
           EERARMLS GRKRK+EKDRL K  E + E      E +SEE   RVELPI+VK+DVQGT 
Sbjct: 477 EERARMLSEGRKRKYEKDRLLKAEEARLEEAAARLETESEEGFVRVELPIVVKSDVQGTA 536

Query: 532 QAVTDALKTLNSPQVFVNVVHVGVGPVSQSDVDLAQACGAYIVGFNVK-NPPSNLSQTAT 591
           QAV DAL+TLNSPQV VN+VH GVG +S SD+DLAQACGA IVGFNVK     NLS  A 
Sbjct: 537 QAVADALRTLNSPQVSVNIVHSGVGAISHSDLDLAQACGACIVGFNVKGGSTGNLS--AA 596

Query: 592 QAGIKIILHRVIYRLLEDIGNLIVDKAPGTSETQVAGEAEVLNIFELKGRSKSKGPDVKI 651
           Q  +K+  HRVIY LLEDIGNLIV+KAPG SE +V+GEAEVL+IF++ G+ +++   V I
Sbjct: 597 QGSVKVFHHRVIYHLLEDIGNLIVEKAPGVSEMEVSGEAEVLSIFKILGKRRTEEDGVNI 656

Query: 652 AGCRVIDGCVSRASTMRLLRSGEVLFEGSCASLKREKQDVDAVKKGSECGLVIHDWDDFQ 711
           AGC+V+DG V R+  MRLLRSGEV+FEGSCASLKREKQDV+ V KG+ECGLV  DW+DF+
Sbjct: 657 AGCKVMDGRVCRSGLMRLLRSGEVVFEGSCASLKREKQDVEQVGKGNECGLVFGDWNDFR 716

Query: 712 VGDVVQCLEQVVRKPKFISSESGAVRIEC 725
           VGDV+QC+E V+RKPKFISSESGAVRIEC
Sbjct: 717 VGDVIQCMEPVIRKPKFISSESGAVRIEC 743

BLAST of MC10g0725 vs. TAIR 10
Match: AT1G17220.1 (Translation initiation factor 2, small GTP-binding protein )

HSP 1 Score: 393.3 bits (1009), Expect = 4.3e-109
Identity = 233/551 (42.29%), Postives = 332/551 (60.25%), Query Frame = 0

Query: 170  EFDPLSIDIAELVAMEVGVNIKRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALRQTS 229
            ++D   +D   +   E+    +     +  +++ RPPVIT+MGHVDHGKT+LLD +R++ 
Sbjct: 466  DYDVEVLDADSVKVEEMAKKRQTFDEEDLDKLEDRPPVITIMGHVDHGKTTLLDYIRKSK 525

Query: 230  VAAREAGGITQHLGAFVVGMASGA---SITFLDTPGHAAFSAMRARGAAVTDIVVLVVAA 289
            VAA EAGGITQ +GA+ V +       S  FLDTPGH AF AMRARGA VTDI ++VVAA
Sbjct: 526  VAASEAGGITQGIGAYKVSVPVDGKLQSCVFLDTPGHEAFGAMRARGARVTDIAIIVVAA 585

Query: 290  DDGVMPQTLEAMAHAKAANVPIVLAINKCDKPAADPERVKLQLASEGLLLEEMGGDVQVV 349
            DDG+ PQT EA+AHAKAA VPIV+AINK DK  A P+RV  +L+S GL+ E+ GGDV +V
Sbjct: 586  DDGIRPQTNEAIAHAKAAAVPIVIAINKIDKEGASPDRVMQELSSIGLMPEDWGGDVPMV 645

Query: 350  HVSALKKTGLDTLEEALLLQAEMMDLKARIDGPAQAYVVEARLDKGRGPLATTIVKAGTL 409
             +SALK   +D L E ++L AE+ +LKA     A+  V+EA LDK +GP AT IV+ GTL
Sbjct: 646  QISALKGENVDDLLETVMLVAELQELKANPHRNAKGIVIEAGLDKAKGPFATFIVQKGTL 705

Query: 410  ESGQFVVVGCEWGRIRAIRDMLGNLTDRAGPAMPVEIEGLRGLPMAGDDIIVVESEERAR 469
            + G  VV G  +G++RA+ D  G   D AGP++PV++ GL  +P+AGD+  +V S + AR
Sbjct: 706  KRGDVVVCGEAFGKVRALFDHSGERVDEAGPSIPVQVIGLNNVPIAGDEFEIVSSLDVAR 765

Query: 470  MLSAGRKRKFEKDRLK-KLSEGKTETEEQSEEV-------IQRVELPIIVKADVQGTVQA 529
             ++  R      +R+  K  +GK      +  V       +   +L II+K DVQG+++A
Sbjct: 766  EMAEARAVSLRDERISAKAGDGKVTLSSLASAVSAKKMSGLDLHQLNIILKVDVQGSIEA 825

Query: 530  VTDALKTLNSPQVFVNVVHVGVGPVSQSDVDLAQACGAYIVGFNVKNPPSNLSQTATQAG 589
            V  AL+ L    V +  +    G VS SDVDLA A  A + GFNVK    ++ + A   G
Sbjct: 826  VRQALQVLPQENVTLKFLLQATGDVSNSDVDLASASEAIVFGFNVK-ASGSVKKAAENKG 885

Query: 590  IKIILHRVIYRLLEDIGNLIVDKAPGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGC 649
            ++I L+RVIY L++D+ N +        E    G AEV   F     S   G   ++AGC
Sbjct: 886  VEIRLYRVIYELIDDVRNAMEGLLESVEEQIPIGSAEVRATF-----SSGSG---RVAGC 945

Query: 650  RVIDGCVSRASTMRLLRSGEVLFEGSCASLKREKQDVDAVKKGSECGLVIHDWDDFQVGD 709
             V +G   +   +R++R G+ +  G   SLKR K++V  V  G ECG+ + D+DD+  GD
Sbjct: 946  MVNEGKFVKDCGIRVVRKGKTVHVGVLDSLKRVKENVKEVSAGLECGIGMDDYDDWIEGD 1005

BLAST of MC10g0725 vs. TAIR 10
Match: AT1G76810.1 (eukaryotic translation initiation factor 2 (eIF-2) family protein )

HSP 1 Score: 128.6 bits (322), Expect = 2.0e-29
Identity = 146/566 (25.80%), Postives = 242/566 (42.76%), Query Frame = 0

Query: 183  AMEVGVNIKRLHSSEGSQIQPRPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQHL 242
            A + G  +    S EG +   R P+  +MGHVD GKT LLD +R T+V   EAGGITQ +
Sbjct: 684  ASKKGKGLAPSESIEGEE-NLRSPICCIMGHVDTGKTKLLDCIRGTNVQEGEAGGITQQI 743

Query: 243  GAFVV---------------GMASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAAD 302
            GA                         +  +DTPGH +F+ +R+RG+++ D+ +LVV   
Sbjct: 744  GATYFPAENIRERTKELKADAKLKVPGLLVIDTPGHESFTNLRSRGSSLCDLAILVVDIM 803

Query: 303  DGVMPQTLEAMAHAKAANVPIVLAINKCD--------KPAADPERVKLQ----------- 362
             G+ PQT+E++   +  N   ++A+NK D        K A   + +K Q           
Sbjct: 804  HGLEPQTIESLNLLRMRNTEFIVALNKVDRLYGWKTCKNAPIVKAMKQQNKDVINEFNLR 863

Query: 363  -------LASEGLLLE------EMGGDVQVVHVSALKKTGLDTLEEALL--LQAEMMDLK 422
                      +GL  E      +MG    +V  SA+   G+  L   L+   Q  M++  
Sbjct: 864  LKNIINEFQEQGLNTELYYKNKDMGDTFSIVPTSAISGEGVPDLLLWLVQWAQKTMVEKL 923

Query: 423  ARIDGPAQAYVVEARLDKGRGPLATTIVKAGTLESG-QFVVVGCEWGRIRAIRDMLGNLT 482
              +D   Q  V+E ++ +G G     ++  G L  G Q VV G +   +  IR +L    
Sbjct: 924  TYVD-EVQCTVLEVKVIEGHGTTIDVVLVNGELHEGDQIVVCGLQGPIVTTIRALL---- 983

Query: 483  DRAGPAMPVEIEG----LRGLPMAGDDIIVVESEERARMLSAGRKRKFEKDRLKKLSEGK 542
                P   + ++G     + +  A    I  +  E A +           D ++ + E  
Sbjct: 984  -TPHPMKELRVKGTYLHYKEIKAAQGIKITAQGLEHA-IAGTALHVVGPDDDIEAIKESA 1043

Query: 543  TETEEQSEEVIQRVELPIIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGVGPVSQSDV 602
             E  E     I +    + V+A   G+++A+ + LK   SP V + V  +G+GPV + DV
Sbjct: 1044 MEDMESVLSRIDKSGEGVYVQASTLGSLEALLEYLK---SPAVKIPVSGIGIGPVHKKDV 1103

Query: 603  DLAQAC------GAYIVGFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVDKA 662
              A          A I+ F+VK   +   + A + G+KI    +IY L  D+    ++  
Sbjct: 1104 MKAGVMLERKKEYATILAFDVK-VTTEARELADEMGVKIFCADIIYHLF-DLFKAYIENI 1163

Query: 663  PGTSETQVAGEAEVLNIFELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVLFE 689
                + + A EA    + ++         D  + G  VI+G + +  T   +   E +  
Sbjct: 1164 KEEKKKESADEAVFPCVLQILPNCVFNKKDPIVLGVDVIEG-ILKIGTPICVPGREFIDI 1223

BLAST of MC10g0725 vs. TAIR 10
Match: AT1G21160.1 (eukaryotic translation initiation factor 2 (eIF-2) family protein )

HSP 1 Score: 127.5 bits (319), Expect = 4.4e-29
Identity = 144/554 (25.99%), Postives = 237/554 (42.78%), Query Frame = 0

Query: 204  RPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQHLGAFVV---------------G 263
            R P+  +MGHVD GKT LLD +R T+V   EAGGITQ +GA                   
Sbjct: 496  RSPICCIMGHVDSGKTKLLDCIRGTNVQEGEAGGITQQIGATFFPAENIRERTKELQANA 555

Query: 264  MASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAHAKAANVPI 323
                  I  +DTPGH +F+ +R+RG+ + D+ +LVV    G+ PQT+E++   +  NV  
Sbjct: 556  KLKVPGILVIDTPGHESFTNLRSRGSNLCDLAILVVDIMRGLEPQTIESLNLLRRRNVKF 615

Query: 324  VLAINKCD------KPAADP--------------------ERVKLQLASEGL------LL 383
            ++A+NK D      K    P                     RV+ Q   +GL        
Sbjct: 616  IIALNKVDRLYGWEKSKNAPIRKTMMQQTGDVVKEFKMRLNRVQNQFQEQGLNSMLYYKN 675

Query: 384  EEMGGDVQVVHVSALKKTGLDTLEEALL--LQAEMMDLKARIDGPAQAYVVEARLDKGRG 443
             EMG  + ++  SA+   G+  L   L+   Q  M++    +D   Q  V+E ++ +G G
Sbjct: 676  REMGETISILPASAISGEGIPDLLLFLVQWAQKTMVEKLTYVD-KVQCTVLEVKVIEGHG 735

Query: 444  PLATTIVKAGTLESG-QFVVVGCEWGRIRAIRDMLG----NLTDRAGPAMP-VEIEGLRG 503
                 ++  G L  G Q VV G +   +  IR +L     N     G  MP  E++  +G
Sbjct: 736  ITVDVVLVNGVLREGDQIVVCGSQGPIVTTIRSLLTPYPMNEMRVTGTYMPHREVKAAQG 795

Query: 504  LPMAGDDIIVVESEERARMLSAGRKRKFEKDRLKKLSEGKTETEEQSEEVIQRVELP--- 563
            + +A   +    +     ++             + + E K    E  E V+ R++     
Sbjct: 796  IKIAAQGLEHAIAGTALHVIGPN----------EDMEEAKKNAMEDIESVMNRIDKSGEG 855

Query: 564  IIVKADVQGTVQAVTDALKTLNSPQVFVNVVHVGVGPVSQSDVDLAQAC------GAYIV 623
            + V+A   G+++A+ + LK   S  V + V  +G+GPV + D+  A          A I+
Sbjct: 856  VYVQASTLGSLEALLEFLK---SSDVKIPVSGIGIGPVHKKDIMKAGVMLEKKKEFATIL 915

Query: 624  GFNVKNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVDKAPGTSETQVAGEAEVLNI 683
             F+VK   +   + A + G+KI     IY L +   + I +      + + A EA    I
Sbjct: 916  AFDVK-ISAEARELADKMGVKIFCDDTIYCLFDKFKSYI-EGIKEEKKKETACEAVFPCI 975

Query: 684  FELKGRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGE----VLFEGSCASLK-REKQD 689
             ++         D  I G +V DG +   + + +++  E     +  G  +S+K      
Sbjct: 976  LQILPNHIYNQRDPIILGVKVNDGILKVGTPICIVKRIENVRVFMDIGRVSSIKNNNNMP 1033

BLAST of MC10g0725 vs. TAIR 10
Match: AT1G76820.1 (eukaryotic translation initiation factor 2 (eIF-2) family protein )

HSP 1 Score: 124.0 bits (310), Expect = 4.9e-28
Identity = 139/545 (25.50%), Postives = 231/545 (42.39%), Query Frame = 0

Query: 204  RPPVITVMGHVDHGKTSLLDALRQTSVAAREAGGITQHLGAFVV---------------G 263
            R  +  +MGHVD GKT LLD +R T+V   EAGGITQ +GA                   
Sbjct: 576  RSIICCIMGHVDSGKTKLLDCIRGTNVQEGEAGGITQQIGATYFPAKNIRERTRELKADA 635

Query: 264  MASGASITFLDTPGHAAFSAMRARGAAVTDIVVLVVAADDGVMPQTLEAMAHAKAANVPI 323
                  +  +DTPGH +F+ +R+RG+++ D+ +LVV    G+ PQT+E++   +  N   
Sbjct: 636  KLKVPGLLVIDTPGHESFTNLRSRGSSLCDLAILVVDITHGLQPQTIESLNLLRMRNTEF 695

Query: 324  VLAINKCD--------KPAADPERVKLQ------------------LASEGLLLE----- 383
            ++A+NK D        K A   + +K Q                     +GL  E     
Sbjct: 696  IIALNKVDRLYGWKTCKNAPIVKAMKQQNKDVINEFNLRLKKIINEFQEQGLNTELYYKN 755

Query: 384  -EMGGDVQVVHVSALKKTGLDTLEEALL--LQAEMMDLKARIDGPAQAYVVEARLDKGRG 443
             +MG    +V  SA+   G+  L   L+   Q  M++    +D   Q  V+E ++ +G G
Sbjct: 756  KDMGETFSIVPTSAISGEGVPDLLLWLVQWAQKTMVEKLTYVD-EVQCTVLEVKVIEGHG 815

Query: 444  PLATTIVKAGTLESG-QFVVVGCEWGRIRAIRDMLGNLTDRAGPAMPVEIEGL----RGL 503
                 ++  G L  G Q VV G +   +  IR +L        P   + ++G     + +
Sbjct: 816  TTIDVVLVNGELHEGDQIVVCGLQGPIVTTIRALL-----TPHPMKELRVKGTYLHHKEI 875

Query: 504  PMAGDDIIVVESEERARMLSAGRKRKFEKDRLKKLSEGKTETEEQSEEVIQRVELPIIVK 563
              A    I  +  E A +           D ++ + E   E  E     I +    + V+
Sbjct: 876  KAAQGIKITAQGLEHA-IAGTSLHVVGPDDDIEAMKESAMEDMESVLSRIDKSGEGVYVQ 935

Query: 564  ADVQGTVQAVTDALKTLNSPQVFVNVVHVGVGPVSQSDVDLAQAC------GAYIVGFNV 623
                G+++A+ + LKT   P V + V  +G+GPV + D+  A          A I+ F+V
Sbjct: 936  TSTLGSLEALLEFLKT---PAVNIPVSGIGIGPVHKKDIMKAGVMLEKKKEYATILAFDV 995

Query: 624  KNPPSNLSQTATQAGIKIILHRVIYRLLEDIGNLIVDKAPGTSETQVAGEAEVLNIFELK 683
            K   +   + A + G+KI    +IY+L      + ++      + + AGEA    + ++ 
Sbjct: 996  K-VTTEARELADEMGVKIFCADIIYQLFNQF-QVYIENIKEEKKKESAGEAVFPCVLQIL 1055

Query: 684  GRSKSKGPDVKIAGCRVIDGCVSRASTMRLLRSGEVLFEGSCASLKREKQDVDAVKKGSE 689
                    D  I G +V DG + +  T   +   E    G  AS++   + VD  +KG E
Sbjct: 1056 PNCVFNKRDPIILGVKVHDG-ILKIGTPICVPGREFTDIGRIASIENNHKPVDYAEKGDE 1107

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A9HF181.4e-13647.42Translation initiation factor IF-2 OS=Gluconacetobacter diazotrophicus (strain A... [more]
A7HZ934.0e-13647.64Translation initiation factor IF-2 OS=Parvibaculum lavamentivorans (strain DS-1 ... [more]
A4XL702.4e-13344.87Translation initiation factor IF-2 OS=Caldicellulosiruptor saccharolyticus (stra... [more]
B8EIA75.4e-13345.21Translation initiation factor IF-2 OS=Methylocella silvestris (strain DSM 15510 ... [more]
Q5FQM37.1e-13345.61Translation initiation factor IF-2 OS=Gluconobacter oxydans (strain 621H) OX=290... [more]
Match NameE-valueIdentityDescription
XP_022136685.10.0100.00uncharacterized protein LOC111008337 [Momordica charantia][more]
XP_008451977.10.090.75PREDICTED: translation initiation factor IF-2 [Cucumis melo][more]
XP_004149759.10.090.88uncharacterized protein LOC101205928 [Cucumis sativus] >KGN53381.1 hypothetical ... [more]
XP_038894926.10.090.62translation initiation factor IF-2 [Benincasa hispida][more]
KAA0044874.10.090.90translation initiation factor IF-2 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A6J1C4N00.0100.00uncharacterized protein LOC111008337 OS=Momordica charantia OX=3673 GN=LOC111008... [more]
A0A1S3BS610.090.75translation initiation factor IF-2 OS=Cucumis melo OX=3656 GN=LOC103493119 PE=3 ... [more]
A0A0A0KZS30.090.88Tr-type G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G050840 P... [more]
A0A5A7TP110.090.90Translation initiation factor IF-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A5D3D1N20.087.67Translation initiation factor IF-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
AT4G11160.12.8e-26269.23Translation initiation factor 2, small GTP-binding protein [more]
AT1G17220.14.3e-10942.29Translation initiation factor 2, small GTP-binding protein [more]
AT1G76810.12.0e-2925.80eukaryotic translation initiation factor 2 (eIF-2) family protein [more]
AT1G21160.14.4e-2925.99eukaryotic translation initiation factor 2 (eIF-2) family protein [more]
AT1G76820.14.9e-2825.50eukaryotic translation initiation factor 2 (eIF-2) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 200..375
e-value: 1.7E-64
score: 218.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 203..394
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 606..709
e-value: 9.1E-31
score: 107.8
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 376..495
e-value: 1.3E-32
score: 114.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 105..126
NoneNo IPR availablePANTHERPTHR43381:SF5TRANSLATION INITIATION FACTOR IF-2, MITOCHONDRIALcoord: 77..715
NoneNo IPR availableCDDcd03692mtIF2_IVccoord: 612..702
e-value: 2.26315E-32
score: 117.979
NoneNo IPR availableCDDcd01887IF2_eIF5Bcoord: 206..370
e-value: 1.0563E-97
score: 296.691
IPR005225Small GTP-binding protein domainTIGRFAMTIGR00231TIGR00231coord: 206..359
e-value: 8.2E-28
score: 95.2
IPR000178Translation initiation factor aIF-2, bacterial-likeTIGRFAMTIGR00487TIGR00487coord: 131..707
e-value: 3.9E-180
score: 598.4
IPR000178Translation initiation factor aIF-2, bacterial-likeHAMAPMF_00100_BIF_2_Bcoord: 23..710
score: 13.900911
IPR023115Translation initiation factor IF- 2, domain 3PFAMPF11987IF-2coord: 500..594
e-value: 7.2E-29
score: 100.1
IPR036925Translation initiation factor IF-2, domain 3 superfamilyGENE3D3.40.50.10050coord: 499..604
e-value: 7.6E-30
score: 105.0
IPR036925Translation initiation factor IF-2, domain 3 superfamilySUPERFAMILY52156Initiation factor IF2/eIF5b, domain 3coord: 490..611
IPR000795Translational (tr)-type GTP-binding domainPFAMPF00009GTP_EFTUcoord: 208..365
e-value: 7.1E-32
score: 110.5
IPR000795Translational (tr)-type GTP-binding domainPROSITEPS51722G_TR_2coord: 203..373
score: 50.358566
IPR015760Translation initiation factor IF- 2PANTHERPTHR43381TRANSLATION INITIATION FACTOR IF-2-RELATEDcoord: 77..715
IPR044145Translation initiation factor IF-2, domain IICDDcd03702IF2_mtIF2_IIcoord: 379..474
e-value: 2.16769E-38
score: 135.63
IPR009000Translation protein, beta-barrel domain superfamilySUPERFAMILY50447Translation proteinscoord: 607..709
IPR009000Translation protein, beta-barrel domain superfamilySUPERFAMILY50447Translation proteinscoord: 377..460

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0725.1MC10g0725.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006413 translational initiation
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0003743 translation initiation factor activity