MC10g0682 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0682
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionsubtilisin-like protease SBT3.4
LocationMC10: 5658804 .. 5664470 (+)
RNA-Seq ExpressionMC10g0682
SyntenyMC10g0682
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACGAATGGAAACTTCAGAAATGGCGGTTGTGGTTCGTGTTCTTCCAAATTCGCTTTACAAAATGCAGACTACAAGAAGTTGGGATTTCCTGGGCCTCTCAACTTCTCGCTCTCAATCCTCCAACCTTCTTCATCGCAGTAAAATGGGGGAAGATGTCATTATAGGCGTCGTTGATTCAGGTACAAAAACAAATTACTTTTGTCCTTATTCAATACCTATTCTATTTCTATCTCGACCCCATTTAAAAAATTGAATGTATCATTATTATTTTTTAAAAAAAAATTGTAAAATCTAAATGTTTTTTTTTTTATTTATTTTTAATAATCAGGAATCTGGCCGGATTCGGAAGCTTTTGGAGATGAAGGACTGGGGCCGGTGCCATCACGATGGAAAGGCAAGTGCAGGAGCAGAATTCAACTCCACAAACTGCAACAGAAAAATCATAGGCGCGCGTTGGTACAATAAAGGCTTCATCGCCGACTTCGGGCGCGAGGCACTGGCGAACGAGTACTTATCCGCAAGAGACTTAAGCGGACACGGAACCCACGCCGCCAGCACAGCCGCAGCCTCCTTGGTGCCGGACGTCAGCTACGGGGGCCTCGGCGCCGGCACGGTGAGGGGCGGCGCACCACGCGCGCGCTTGGCCATATACAAGGCATTGTGGTCGGCGACGGGGATAGGGTCGACGGCGGACATATTGAAGGCCATTGACGAGGCCATTCACAATGGCGTGGATGTGCTGTCTCTGTCGCTGGCGGGTGGGCTGCCTATTTTTCCCGAGTTCAGCGAGGAGAGCGGGGTTGCGATTGGGTCGTTTCATGCCATCGCGAGGGGCATTTCTGTGGTGTGTGCGGGTGGGAATGATGGCCCCAATAAGCAGACCATTGTCAACACTGCGCCTTGGATTTTCACCGTGGCTGCCTCTACAATGGATAGGGCTTTTCTTTCCTCCATTACTCTTGGAGATAATGCCAATTATTTGGTAAGCCCGTGTGAACTCAACCTAACTCTCAGGTTGGATATAATTTAGATGCCTGCTGGTAAATAGATATATTTTTACTTGCCACTATTTTTTTTTGGAAAAAGATCACTTCATTATATATGGAGAACATACAACAAAAGAGGGAAGATTAAATAAAAGCTCTCCAATTTAGAGAAATTATAGAAGGATCATGATTACAAAATAAAAAGATTTTGATGAACAAAGAGATGCACATAGAATAATGTCTTCCCAGATGTTGGTCAAAGATTTTCTTTATCTAAAAATATGCGACTGTTTCCTCTCGAGCCAAAATCCATGATTCTTCTAGGCGAGAAAGACCAAATTTCATGATCCCCGTCCCATGCTTCAAAATCAGATAAATCTTTCCTGTTAGAAAGAGCATAAAATATGGGAAAAAGCAGGTAAAGAGGGCCTTTTTCCAGTCCAATTAGAAAAGGTTATCTCTTCCGTTGCCAATCCGCCACTGAATATGATTGCTGAAAGAATCTTTCTGTTTCAGAATATAGAACCAGGGGGATTTTGAACTTTCATATCTACTATATGAATGTATAATATCAAAAGCCTTAGTAGAGTATTTAGGAAATATAAGAGAAAACCAGAGACTATCTTTTTCAGTTAAATACCTCCATATCCATTTACTGAGAAGGGCCGAATTTGTAGCTGCAATGTTCCCAATGTCGAGGCCTTCACTGTCTTTTGGCACGGTGACAATATCCCAGCGGACTAAGTGGGAACTTTTGGAATTTTCACCCTCTCCCCATAGAAAATTACGCATCAATCTCTCTATATTTTTACACGCTGCTATAGGGACTTTGAAGACAGATAAGTAATAAATAGGGAGGTTACTTGCCACTATTAAACAATTGACACTGAAATAAGATAAATGCATGAAGAAATGTTAAGGATAAGGGACCACACTCGTGAGGTTTCGTTTTTGGTATCACCAAATAAAGACCTCATTTCCTTGGAGATTGTGTACTTAGCGTATATATCTAAGATCTTCCATTTCCTTAACTAATGTGAGATTTCGATTGTATTTCAAATTATTCTCACTTCAATCAAAAGATCATCGGTGAGATTTGATCCCACATTTAGCAAGAAGAAGTAAAAAAATATAACGAATAGTTGTCAAATTAAAAGGTTGTTGAAAGATGCCCACTTGATGAAGTTTATGGCTTTTTTTTCTTTTTCTGTATAGGGCCAAAGTTTGTTCTCTGCCAAAAAGGACGTTGCCGGCAATTTGGTGTGTTCCACGACCGGAAGGTTGGTTTTCATGAAAATTTTTGGGATAAGACATAAGTGCTTATGATTCTTCAGAAAATTACAACTCTTTATTTTATTTTTTTATTTATTAGTGTCTGGGTCAATTTAAGAAATAGATATAATATAGGGGTAGTTACAAATATGACAAAAAAATTTAAGGAAATAACGAATTTAGCCTCTACATTTTAGATTTGCAAATATAGCAAAAATTTTAGGAAAAACGAAGGTCCAATAATAAAATGTCAAATTTACCCTCGTAGCATTGATATACCATTGATATAGCGCGCGCATATATATACATATATACACACATATACTTGATATACATTTGATTTACTACTGATATATTATTGATATAACTATGAGATCTACTAATATATCTTCTTATATAATTATATTATTTACTATTTATTGATTTACCACTAATATATGTATAATATATTATTGATATGCCTTTGATATACCACTGATATAATACTGATATACAGTTGATTTACTTTTGATATATCTATGATATTTACCAATATAACTTTTAATATGGTTTACTACTGATACACGTATGATATAACTATGATTTCTACTGATATAAATTTTTATATGATTATATAATTTACCATTGATTTACCATGCTGATATAACACTTATATATCTGTGATATATACTTGATATGTTTTAAGTTACCACTTATATAACTATGATATGTACTAATATAACTTCTCATATGATTATATGATTTAACACTTGTATAACTTTGATACATCCATGATATATACTTAATACGTCTCTAATTTACCATTGATATAACCATGATATCTAACATTGATGAATCTGTGATCTATATCTGATGTATATCAGTGGCACAGATGAACATAAAATGTACATCAGAGATACATCGAATATATATCATATAAGATATGTATAGGTATATCATTACAAATATATTTAGTATATCATAAATTAGAGATACATTAGATGTATATTAGATGTATAATACGTACATCTGATGTATATCAGAGATAGATCGGATGTACATCAGATGTAGATAAGAAGTATATCAGAGATATATCGGGCGTATATCAGATATACATCCATTGGGCTTATATCAGATATACATTAGGTGTGTACCAGATATCACAAGGGTAAAACTGGAATAATAAAAATTTCGAAAATAAAAATTCAAATATTTTGTCATATTTACAAAATAGAAGAGTGGTTGACATTTTTGTAAATATATATAAAAGTTTAGGTGCCCAATACAATTTTCCTATAATATAATATAATATTGTAGGTTGGTAGTTAGATTTGAAATCACTGTCTCTAAATTCTTTTGAATATTTTTAGCGTCTTTTGGACCAATGACTGGCTGAAGATGGTTATGGATTTATCTTTCTTAATTATAGATTTTTTTAATGGTAATAAACAGGAGAATCTACTTATATCAATAGCCCAAGTTGCATCCCATTTTAATATCCAAGAGCATTTTTTTTTGTTGGTTGTCAGTTTTAGTGATTTAACTTTTGAATAGAAATTATTTTCTACTTTTATTAGTTGTTGAGGGTGGTGATGCAGGTTATGTTCATGCGATGGGATTTCGGGTAATGGCATTCTCTGGTTCTATGCTTCGATAAATTAGCGAATGCGGCGACTACATTAACAGCAGTTGCAGCGGTGGAAGCAGCAAACGGGATTGGGATAATCGTGGCCGGCCAACACGACGACATCTTGTTTCCATGTGGTCAAGCTCATCACTTTCCATGCATCGTAGTAGATCCTGACGTGGCCACTAAAATTTTTTACTACGCCTCGGACGCCACGTATGATGAACAAACACATTTTTCCTCCTAATCTAATAATGGAATAATATTGATCACTCAATTTTTGTTTGCAAACTTAAATGAATGTGGTATGTGGTATATATGGTAAGGTAATTAGTTCTGTTTTTGATTTTGTTGTTTGATCTTGTGAAGTAATCCACGGTGAGGGTGGGGCGCGCAACAACGTCGTGGGGAAGCATATGTCAACCAACATAGCAGTTTTCTCATCTAGAGGTCCTAATTCTGCTTCTCCCGCAATTCTCAAGGTACCTTCTTTATCATCCATATTATTTATGTGCCTAAGATTATAGTAATAAAAGTTAGGCCCTTCACTTTTATTGAAGAGTCTCTAGCTAGTTCCTAAACTTCTAATGAATTTCAATTATACCCATTTTTCTTTCATATATATATATATATCCTTTAATTTTCCTTTTAATTTTGATTTTAATATAAGGTCCTACCCTAAAAGAAAGAATATTTTATATACTTAAAGTGTCAGTATTCATCTGTGTGATCACCCCTAAAATTTTAACCGTTTTGTTTAGAAAATCTCTTTTTCTATATTCTTACTTCCCCTAAAAAATTTAATATTAATCAACAAAATGATAAGCAATAACCATTAAACAGTAATTTTATTTATTTATTTTTAATAATGTTTCGTTGTAAACTTATTCAAAATATATATCAACCCCAAATTATTTATATGAAAACGAAAAATTATAGGTCCACGACCACCTATGCCACCTTACACTTCCTCAATTGCCTTGCTGGCATCATGTCTGTTCGATCTACGATTTTTCAAACATTTTTATTTGAAATAGTTTATATTCCCGACAAAAAACTAAGACATTTAATAAATTTTGATCGGTGTTGGTGAGCAGCCATACATAGCCGCGCCTGGATCCAACATTCTAGCTGCCATTTCACGGCACCACATCTTCAGCGTCAAAGAGTTCGCGTTCGATTCAGGAACTTCCATGGCCACACCTCATATCTCTGGCATTGTGGCTCTCCTTAAATCCGTGCATCCCACTTGGTCACCCGCCGCCATTAAATCAGCCCTCATCACCACTGGTAATTAACATCCATTCCAATTTTATGATGCTCATGAACTTTAGAATTACCACCCTTTTTAAAATGCAGCACAAGAAAAAGACCACTGGGGGCGCCCATTTTGGCTGATGGCTCTCCTCCCAAGTTGGCCGACCCATTTGACTACGGCGGCGGATTGGTGAACCCCAACGCCGCTGCAGACCCAGGTCTAATCTACGATCTAGCCATTGTAGACTATGTCAAGTACTACCTGTGCGGCATGGGGTATAAGACCTCACATATTTCTCAATTAATGCAGCAGAAAACAGAGTGCCCGTCACAGAGGTTATCGGTGTCGGATTTGCCGAGCATTACAGTACCAGCTCTAAGAAACTCCACCACCGTGACTCGTACGGTGACGAATGTGGGAAATTCGAGCTCGGTGTACAGGGCAGTGATCCGAGCCCCATCAGGGACGAGGGTGAGTGTGGAGCCCCAGGTGCCGGTGTTCAACCCCGAAGTGAAGAAGATTTTGTTCAAGGTTACCATTTCT

mRNA sequence

CAACGAATGGAAACTTCAGAAATGGCGGTTGTGGTTCGTGTTCTTCCAAATTCGCTTTACAAAATGCAGACTACAAGAAGTTGGGATTTCCTGGGCCTCTCAACTTCTCGCTCTCAATCCTCCAACCTTCTTCATCGCAGTAAAATGGGGGAAGATGTCATTATAGGCGTCGTTGATTCAGGAATCTGGCCGGATTCGGAAGCTTTTGGAGATGAAGGACTGGGGCCGGTGCCATCACGATGGAAAGGCAAGTGCGGAGCAGAATTCAACTCCACAAACTGCAACAGAAAAATCATAGGCGCGCGTTGGTACAATAAAGGCTTCATCGCCGACTTCGGGCGCGAGGCACTGGCGAACGAGTACTTATCCGCAAGAGACTTAAGCGGACACGGAACCCACGCCGCCAGCACAGCCGCAGCCTCCTTGGTGCCGGACGTCAGCTACGGGGGCCTCGGCGCCGGCACGGTGAGGGGCGGCGCACCACGCGCGCGCTTGGCCATATACAAGGCATTGTGGTCGGCGACGGGGATAGGGTCGACGGCGGACATATTGAAGGCCATTGACGAGGCCATTCACAATGGCGTGGATGTGCTGTCTCTGTCGCTGGCGGGTGGGCTGCCTATTTTTCCCGAGTTCAGCGAGGAGAGCGGGGTTGCGATTGGGTCGTTTCATGCCATCGCGAGGGGCATTTCTGTGGTGTGTGCGGGTGGGAATGATGGCCCCAATAAGCAGACCATTGTCAACACTGCGCCTTGGATTTTCACCGTGGCTGCCTCTACAATGGATAGGGCTTTTCTTTCCTCCATTACTCTTGGAGATAATGCCAATTATTTGGGGGATTTTGAACTTTCATATCTACTATATGAATGTATAATATCAAAAGCCTTAGTAGAGTATTTAGGAAATATAAGAGAAAACCAGAGACTATCTTTTTCAGTTAAATACCTCCATATCCATTTACTGAGAAGGGCCGAATTTGTAGCTGCAATGTTCCCAATGTCGAGGCCTTCACTGTCTTTTGGCACGGTGACAATATCCCAGCGGACTAAAGATATATCGGGCGTATATCAGATATACATCCATTGGGCTTATATCAGATATACATTAGTAATCCACGGTGAGGGTGGGGCGCGCAACAACGTCGTGGGGAAGCATATGTCAACCAACATAGCAGTTTTCTCATCTAGAGGTCCTAATTCTGCTTCTCCCGCAATTCTCAAGCCATACATAGCCGCGCCTGGATCCAACATTCTAGCTGCCATTTCACGGCACCACATCTTCAGCGTCAAAGAGTTCGCGTTCGATTCAGGAACTTCCATGGCCACACCTCATATCTCTGGCATTGTGGCTCTCCTTAAATCCGTGCATCCCACTTGGTCACCCGCCGCCATTAAATCAGCCCTCATCACCACTGAATTACCACCCTTTTTAAAATGCAGCACAAGAAAAAGACCACTGGGGGCGCCCATTTTGGCTGATGGCTCTCCTCCCAAGTTGGCCGACCCATTTGACTACGGCGGCGGATTGGTGAACCCCAACGCCGCTGCAGACCCAGGTCTAATCTACGATCTAGCCATTGTAGACTATGTCAAGTACTACCTGTGCGGCATGGGGTATAAGACCTCACATATTTCTCAATTAATGCAGCAGAAAACAGAGTGCCCGTCACAGAGGTTATCGGTGTCGGATTTGCCGAGCATTACAGTACCAGCTCTAAGAAACTCCACCACCGTGACTCGTACGGTGACGAATGTGGGAAATTCGAGCTCGGTGTACAGGGCAGTGATCCGAGCCCCATCAGGGACGAGGGTGAGTGTGGAGCCCCAGGTGCCGGTGTTCAACCCCGAAGTGAAGAAGATTTTGTTCAAGGTTACCATTTCT

Coding sequence (CDS)

CAACGAATGGAAACTTCAGAAATGGCGGTTGTGGTTCGTGTTCTTCCAAATTCGCTTTACAAAATGCAGACTACAAGAAGTTGGGATTTCCTGGGCCTCTCAACTTCTCGCTCTCAATCCTCCAACCTTCTTCATCGCAGTAAAATGGGGGAAGATGTCATTATAGGCGTCGTTGATTCAGGAATCTGGCCGGATTCGGAAGCTTTTGGAGATGAAGGACTGGGGCCGGTGCCATCACGATGGAAAGGCAAGTGCGGAGCAGAATTCAACTCCACAAACTGCAACAGAAAAATCATAGGCGCGCGTTGGTACAATAAAGGCTTCATCGCCGACTTCGGGCGCGAGGCACTGGCGAACGAGTACTTATCCGCAAGAGACTTAAGCGGACACGGAACCCACGCCGCCAGCACAGCCGCAGCCTCCTTGGTGCCGGACGTCAGCTACGGGGGCCTCGGCGCCGGCACGGTGAGGGGCGGCGCACCACGCGCGCGCTTGGCCATATACAAGGCATTGTGGTCGGCGACGGGGATAGGGTCGACGGCGGACATATTGAAGGCCATTGACGAGGCCATTCACAATGGCGTGGATGTGCTGTCTCTGTCGCTGGCGGGTGGGCTGCCTATTTTTCCCGAGTTCAGCGAGGAGAGCGGGGTTGCGATTGGGTCGTTTCATGCCATCGCGAGGGGCATTTCTGTGGTGTGTGCGGGTGGGAATGATGGCCCCAATAAGCAGACCATTGTCAACACTGCGCCTTGGATTTTCACCGTGGCTGCCTCTACAATGGATAGGGCTTTTCTTTCCTCCATTACTCTTGGAGATAATGCCAATTATTTGGGGGATTTTGAACTTTCATATCTACTATATGAATGTATAATATCAAAAGCCTTAGTAGAGTATTTAGGAAATATAAGAGAAAACCAGAGACTATCTTTTTCAGTTAAATACCTCCATATCCATTTACTGAGAAGGGCCGAATTTGTAGCTGCAATGTTCCCAATGTCGAGGCCTTCACTGTCTTTTGGCACGGTGACAATATCCCAGCGGACTAAAGATATATCGGGCGTATATCAGATATACATCCATTGGGCTTATATCAGATATACATTAGTAATCCACGGTGAGGGTGGGGCGCGCAACAACGTCGTGGGGAAGCATATGTCAACCAACATAGCAGTTTTCTCATCTAGAGGTCCTAATTCTGCTTCTCCCGCAATTCTCAAGCCATACATAGCCGCGCCTGGATCCAACATTCTAGCTGCCATTTCACGGCACCACATCTTCAGCGTCAAAGAGTTCGCGTTCGATTCAGGAACTTCCATGGCCACACCTCATATCTCTGGCATTGTGGCTCTCCTTAAATCCGTGCATCCCACTTGGTCACCCGCCGCCATTAAATCAGCCCTCATCACCACTGAATTACCACCCTTTTTAAAATGCAGCACAAGAAAAAGACCACTGGGGGCGCCCATTTTGGCTGATGGCTCTCCTCCCAAGTTGGCCGACCCATTTGACTACGGCGGCGGATTGGTGAACCCCAACGCCGCTGCAGACCCAGGTCTAATCTACGATCTAGCCATTGTAGACTATGTCAAGTACTACCTGTGCGGCATGGGGTATAAGACCTCACATATTTCTCAATTAATGCAGCAGAAAACAGAGTGCCCGTCACAGAGGTTATCGGTGTCGGATTTGCCGAGCATTACAGTACCAGCTCTAAGAAACTCCACCACCGTGACTCGTACGGTGACGAATGTGGGAAATTCGAGCTCGGTGTACAGGGCAGTGATCCGAGCCCCATCAGGGACGAGGGTGAGTGTGGAGCCCCAGGTGCCGGTGTTCAACCCCGAAGTGAAGAAGATTTTGTTCAAGGTTACCATTTCT

Protein sequence

QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDSGIWPDSEAFGDEGLGPVPSRWKGKCGAEFNSTNCNRKIIGARWYNKGFIADFGREALANEYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALWSATGIGSTADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISVVCAGGNDGPNKQTIVNTAPWIFTVAASTMDRAFLSSITLGDNANYLGDFELSYLLYECIISKALVEYLGNIRENQRLSFSVKYLHIHLLRRAEFVAAMFPMSRPSLSFGTVTISQRTKDISGVYQIYIHWAYIRYTLVIHGEGGARNNVVGKHMSTNIAVFSSRGPNSASPAILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGIVALLKSVHPTWSPAAIKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGGLVNPNAAADPGLIYDLAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSDLPSITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKILFKVTIS
Homology
BLAST of MC10g0682 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 6.8e-148
Identity = 315/657 (47.95%), Postives = 404/657 (61.49%), Query Frame = 0

Query: 1   QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDS 60
           Q  + SE+  VV+V+PN+LY+M TTR+WD+LG+S   S S  LL ++ MG +VI+GV+DS
Sbjct: 86  QAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVSPGNSDS--LLQKANMGYNVIVGVIDS 145

Query: 61  GIWPDSEAFGDEGLGPVPSRWKGKC--GAEFN-STNCNRKIIGARWYNKGFIADFG--RE 120
           G+WP+SE F D+G GP+PSRWKG C  G  FN S +CNRK+IGA+++  G +A+FG    
Sbjct: 146 GVWPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFGVVNR 205

Query: 121 ALANEYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALWSAT 180
               EYLS RD +GHGTH AST   S +P+VSY GLG GT RGGAP   +A+YKA WS  
Sbjct: 206 TQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKACWS-- 265

Query: 181 GIGSTADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISVVCA 240
           G  S AD+LKA+DEAIH+GVD+LSLSL   +P+FPE    S   +G+FHA+A+GI VV A
Sbjct: 266 GYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETEHTS---VGAFHAVAKGIPVVIA 325

Query: 241 GGNDGPNKQTIVNTAPWIFTVAASTMDRAFLSSITLGDNANYLGDFELSYLLYECIISKA 300
            GN GP  QTI N APW+ TVAA+T DR+F ++ITLG+N   LG         + I    
Sbjct: 326 AGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITILG---------QAIYGGP 385

Query: 301 LVEYLGNIRENQRLSFSVKYLHIHLLRRAE-FVAAMFPMSRPSLS----------FGTVT 360
            + ++G       LS   + L  +     E  V   F  S PS +           G + 
Sbjct: 386 ELGFVGLTYPESPLSGDCEKLSANPNSTMEGKVVLCFAASTPSNAAIAAVINAGGLGLIM 445

Query: 361 ISQRTKDISGVYQIYIHWA------------YIRYTLVIHGEGGARNNVVGKHMSTNIAV 420
               T  ++   +    W             YIR T     +  A   + G+ +ST +A 
Sbjct: 446 AKNPTHSLTPTRK--FPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSVSTKVAT 505

Query: 421 FSSRGPNSASPAILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGIVALL 480
           FSSRGPNS SPAILKP IAAPG NILAAIS +   +   FA  SGTSMATP +SG+V LL
Sbjct: 506 FSSRGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLL 565

Query: 481 KSVHPTWSPAAIKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGGLVNP 540
           KS+HP WSP+AIKSA++TT        + R  P G PI ADGS  KLADPFDYGGGL+NP
Sbjct: 566 KSLHPDWSPSAIKSAIVTT--------AWRTDPSGEPIFADGSSRKLADPFDYGGGLINP 625

Query: 541 NAAADPGLIYDLAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSD--LPSITVP 600
             A  PGLIYD+   DYV  Y+C + Y    IS+++ + T CP+ + SV D  LPSIT+P
Sbjct: 626 EKAVKPGLIYDMTTDDYV-MYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIP 685

Query: 601 ALRNSTTVTRTVTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKILFKVTIS 628
            LR   T+TRTVTNVG  +SVY+ VI  P+G  V+V P   VF+    K  F V +S
Sbjct: 686 NLRGEVTLTRTVTNVGPVNSVYKVVIDPPTGINVAVTPAELVFDYTTTKRSFTVRVS 715

BLAST of MC10g0682 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 1.2e-147
Identity = 303/667 (45.43%), Postives = 415/667 (62.22%), Query Frame = 0

Query: 1   QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDS 60
           Q  + +++  VV V+P+S YK+ TTR+WD+LGLS +  +S  LLH + MGE +IIGV+D+
Sbjct: 91  QAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKS--LLHETNMGEQIIIGVIDT 150

Query: 61  GIWPDSEAFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIADFGREALA 120
           G+WP+SE F D G GPVPS WKG C  G  FNS+NCN+K+IGA+++  GF+A+       
Sbjct: 151 GVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKLIGAKYFINGFLAENESFNST 210

Query: 121 N--EYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALW---- 180
           N  +++S RDL GHGTH ++ A  S VP++SY GL  GTVRGGAPRA +A+YKA W    
Sbjct: 211 NSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAMYKACWYLDD 270

Query: 181 SATGIGSTADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISV 240
             T   S+ADILKA+DEA+H+GVDVLS+SL   +P++ E     G+  G+FHA+ +GI+V
Sbjct: 271 DDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGETDIRDGITTGAFHAVLKGITV 330

Query: 241 VCAGGNDGPNKQTIVNTAPWIFTVAASTMDRAFLSSITLGDNANYLGDFELSYLLYE--- 300
           VC+GGN GP+  T+ NTAPWI TVAA+T+DR+F + +TLG+N   LG       +Y    
Sbjct: 331 VCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLTLGNNKVILGQ-----AMYTGPG 390

Query: 301 -CIISKALVEYLGNIRENQRLSFSVKYLHIHLLRRAEFVAAMFPMSRPSLSFGTVTISQR 360
               S    E  GN   N+  S + + L  +  R  E    +   + P      ++ ++ 
Sbjct: 391 LGFTSLVYPENPGN--SNESFSGTCEELLFNSNRTMEGKVVLCFTTSP-YGGAVLSAARY 450

Query: 361 TKDISGV---------YQIY----------IHWAYIRYTLVIHGEGGA-------RNNVV 420
            K   G+         Y I           + W      L+     G+          +V
Sbjct: 451 VKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKTLV 510

Query: 421 GKHMSTNIAVFSSRGPNSASPAILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMAT 480
           G+ + T +A FSSRGPNS +PAILKP IAAPG +ILAA + +  FS + F   SGTSMA 
Sbjct: 511 GQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAA-TTNTTFSDQGFIMLSGTSMAA 570

Query: 481 PHISGIVALLKSVHPTWSPAAIKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADP 540
           P ISG+ ALLK++H  WSPAAI+SA++TT        + +  P G  I A+GSPPKLADP
Sbjct: 571 PAISGVAALLKALHRDWSPAAIRSAIVTT--------AWKTDPFGEQIFAEGSPPKLADP 630

Query: 541 FDYGGGLVNPNAAADPGLIYDLAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVS 600
           FDYGGGLVNP  +A+PGL+YD+ + DYV  Y+C +GY  + ISQL+ + T C + + SV 
Sbjct: 631 FDYGGGLVNPEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQLIGKTTVCSNPKPSVL 690

Query: 601 D--LPSITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKI 628
           D  LPSIT+P L++  T+TRTVTNVG  +SVYR  +  P G +V+V P+  VFN   KK+
Sbjct: 691 DFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNSTTKKV 737

BLAST of MC10g0682 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 2.8e-146
Identity = 307/661 (46.44%), Postives = 413/661 (62.48%), Query Frame = 0

Query: 1   QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDS 60
           Q  + +++  VV V P+S Y++ TTR+WD+LGLS +  +  NLL+ + MGE+VIIG+VDS
Sbjct: 87  QAKKLADLPEVVHVTPDSFYQLDTTRTWDYLGLSVANPK--NLLNDTNMGEEVIIGIVDS 146

Query: 61  GIWPDSEAFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIA--DFGREA 120
           G+WP+SE F D G+GPVPS WKG C  G  F S+ CN+K+IGA+++  GF+A  +     
Sbjct: 147 GVWPESEVFNDNGIGPVPSHWKGGCVSGENFTSSQCNKKLIGAKYFINGFLATHESFNST 206

Query: 121 LANEYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALWSATG 180
            + +++S RD SGHGTH A+ A  S VP +SY GL  GTVRGGAPRAR+A+YKA W    
Sbjct: 207 ESLDFISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRGGAPRARIAMYKACWYLDR 266

Query: 181 IG----STADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISV 240
                 S+ADILKA+DEA+H+GVDVLSLS+    P FPE    + +A G+FHA+ +GI+V
Sbjct: 267 FDINTCSSADILKAMDEAMHDGVDVLSLSIGYRFPYFPETDVRAVIATGAFHAVLKGITV 326

Query: 241 VCAGGNDGPNKQTIVNTAPWIFTVAASTMDRAFLSSITLGDNANYLGDFELSYLLYEC-I 300
           VC+GGN GP  QT+ NTAPWI TVAA+T+DR+F + ITLG+N   LG  +  Y   E   
Sbjct: 327 VCSGGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPITLGNNKLILG--QAMYTGPELGF 386

Query: 301 ISKALVEYLGNIREN-----QRLSFSVKY-------LHIHLLRRAEFVAAMFPMSRPSLS 360
            S    E  GN  E+     + L F+  +       L      R   V++     + +  
Sbjct: 387 TSLVYPENPGNSNESFSGDCELLFFNSNHTMAGKVVLCFTTSTRYITVSSAVSYVKEAGG 446

Query: 361 FGTVTISQRTKDISGVYQIYIHWA-----------YIRYTLVIHGEGGARNNVVGKHMST 420
            G +       ++S     +   A           YIR T +   +      +VG+ + T
Sbjct: 447 LGVIVARNPGDNLSPCEDDFPCVAVDYELGTDILLYIRSTGLPVVKIQPSKTLVGQPVGT 506

Query: 421 NIAVFSSRGPNSASPAILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGI 480
            +A FSSRGPNS  PAILKP IAAPG +ILAA + +  F+ + F F SGTSMA P ISG+
Sbjct: 507 KVADFSSRGPNSIEPAILKPDIAAPGVSILAATTTNKTFNDRGFIFLSGTSMAAPTISGV 566

Query: 481 VALLKSVHPTWSPAAIKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGG 540
           VALLK++H  WSPAAI+SA++TT        + R  P G  I A+GSP KLADPFDYGGG
Sbjct: 567 VALLKALHRDWSPAAIRSAIVTT--------AWRTDPFGEQIFAEGSPRKLADPFDYGGG 626

Query: 541 LVNPNAAADPGLIYDLAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSD--LPS 600
           LVNP  AA PGL+YDL + DYV  Y+C +GY  + ISQL+ + T C + + SV D  LPS
Sbjct: 627 LVNPEKAAKPGLVYDLGLEDYV-LYMCSVGYNETSISQLVGKGTVCSNPKPSVLDFNLPS 686

Query: 601 ITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKILFKVTI 628
           IT+P L++  T+TRT+TNVG   SVY+ VI  P G +V+V P+  +FN   K++ FKV +
Sbjct: 687 ITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVTPETLLFNSTTKRVSFKVKV 734

BLAST of MC10g0682 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 1.8e-145
Identity = 308/659 (46.74%), Postives = 411/659 (62.37%), Query Frame = 0

Query: 1   QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDS 60
           Q  + +++  VV V+P+  +++ TTR+W++LGLS++  +  NLL+ + MG+ VIIGV+D+
Sbjct: 89  QAKKIADLPEVVHVIPDGFHELATTRTWEYLGLSSANPK--NLLNDTNMGDQVIIGVIDT 148

Query: 61  GIWPDSEAFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIADFG--REA 120
           G+WP+SE+F D G+GP+P +WKG C  G  F ST+CNRK+IGA+++  GF+A+       
Sbjct: 149 GVWPESESFNDNGVGPIPRKWKGGCESGENFRSTDCNRKLIGAKYFINGFLAENKGFNTT 208

Query: 121 LANEYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALW---S 180
            + +Y+SARD  GHGTH AS A  S VP+VSY GL  GT+RGGAPRAR+A+YKA W    
Sbjct: 209 ESRDYISARDFDGHGTHVASIAGGSFVPNVSYKGLAGGTLRGGAPRARIAMYKACWFHEE 268

Query: 181 ATGIG-STADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISV 240
             G+  S +DI+KAIDEAIH+GVDVLS+SL G +P+  E       A G FHA+A+GI V
Sbjct: 269 LKGVTCSDSDIMKAIDEAIHDGVDVLSISLVGQIPLNSETDIRDEFATGLFHAVAKGIVV 328

Query: 241 VCAGGNDGPNKQTIVNTAPWIFTVAASTMDRAFLSSITLGDN------ANYLG-DFELSY 300
           VCAGGNDGP  QT+VN APWI TVAA+T+DR+F + ITLG+N      A Y G +  L+ 
Sbjct: 329 VCAGGNDGPAAQTVVNIAPWILTVAATTLDRSFPTPITLGNNKVILGQATYTGPELGLTS 388

Query: 301 LLYECIISKALVEYLG-----NIRENQR------LSFSVKYLHIHLLRRAEFVAAMFPMS 360
           L+Y          + G     N+  N        L F+    +  + R A FV A   + 
Sbjct: 389 LVYPENARNNNETFSGVCESLNLNPNYTMAMKVVLCFTASRTNAAISRAASFVKAAGGLG 448

Query: 361 RPSLSFGTVTISQRTKDISGV---YQIYIH-WAYIRYTLVIHGEGGARNNVVGKHMSTNI 420
                    T+S    D   V   Y++     +YIR T     +      + G+ + T +
Sbjct: 449 LIISRNPVYTLSPCNDDFPCVAVDYELGTDILSYIRSTRSPVVKIQRSRTLSGQPVGTKV 508

Query: 421 AVFSSRGPNSASPAILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGIVA 480
             FSSRGPNS SPAILKP IAAPG  ILAA S +   +V  FA  SGTSMATP ISG++A
Sbjct: 509 VNFSSRGPNSMSPAILKPDIAAPGVRILAATSPNDTLNVGGFAMLSGTSMATPVISGVIA 568

Query: 481 LLKSVHPTWSPAAIKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGGLV 540
           LLK++HP WSPAA +SA++TT        + R  P G  I A+GS  K++DPFDYGGG+V
Sbjct: 569 LLKALHPEWSPAAFRSAIVTT--------AWRTDPFGEQIFAEGSSRKVSDPFDYGGGIV 628

Query: 541 NPNAAADPGLIYDLAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSD--LPSIT 600
           NP  AA+PGLIYD+   DY+  YLC  GY  S ISQL+ Q T C + + SV D  LPSIT
Sbjct: 629 NPEKAAEPGLIYDMGPQDYI-LYLCSAGYNDSSISQLVGQITVCSNPKPSVLDVNLPSIT 688

Query: 601 VPALRNSTTVTRTVTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKILFKVTIS 628
           +P L++  T+TRTVTNVG   SVY+  +  P G RV V P+  VFN +   + F V +S
Sbjct: 689 IPNLKDEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVVTPETLVFNSKTISVSFTVRVS 736

BLAST of MC10g0682 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 4.1e-145
Identity = 302/659 (45.83%), Postives = 408/659 (61.91%), Query Frame = 0

Query: 1   QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDS 60
           Q  + ++   VV V+ +S Y++ TTR+WD+LGLS   +  +NLL+ + MG+ VIIG +D+
Sbjct: 86  QAKKLADSPEVVHVMADSFYELATTRTWDYLGLSV--ANPNNLLNDTNMGDQVIIGFIDT 145

Query: 61  GIWPDSEAFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIADFG--REA 120
           G+WP+SE+F D G+GP+PS WKG C  G +F STNCNRK+IGA+++  GF+A+       
Sbjct: 146 GVWPESESFNDNGVGPIPSHWKGGCESGEKFISTNCNRKLIGAKYFINGFLAENEGFNTT 205

Query: 121 LANEYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALWSATG 180
            + +Y+SARD  GHGTH AS A  S VP++SY GL  G +RGGAPRAR+AIYKA W    
Sbjct: 206 ESRDYISARDFIGHGTHTASIAGGSFVPNISYKGLAGGNLRGGAPRARIAIYKACWYVDQ 265

Query: 181 IG----STADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISV 240
           +G    S++DILKA+DE++H+GVDVLSLSL   +P++PE      +A G+FHA+A+GI V
Sbjct: 266 LGAVACSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPETDLRDRIATGAFHAVAKGIIV 325

Query: 241 VCAGGNDGPNKQTIVNTAPWIFTVAASTMDRAFLSSITLGDNANYLG-------DFELSY 300
           VCAGGN GP  QT++NTAPWI TVAA+T+DR+F + ITLG+    LG       +   + 
Sbjct: 326 VCAGGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPITLGNRKVILGQALYTGQELGFTS 385

Query: 301 LLYECIISKALVEYLG-----NIRENQR------LSFSVKYLHIHLLRRAEFVAAMFPMS 360
           L+Y          + G     N+  N+       L F+   L   + R A +V A   + 
Sbjct: 386 LVYPENAGFTNETFSGVCERLNLNPNRTMAGKVVLCFTTNTLFTAVSRAASYVKAAGGLG 445

Query: 361 RPSLSFGTVTISQRTKDISGV---YQIYIH-WAYIRYTLVIHGEGGARNNVVGKHMSTNI 420
                     ++    D   V   Y++      YIR T     +      +VG+ + T +
Sbjct: 446 VIIARNPGYNLTPCRDDFPCVAIDYELGTDVLLYIRSTRSPVVKIQPSRTLVGQPVGTKV 505

Query: 421 AVFSSRGPNSASPAILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGIVA 480
           A FSSRGPNS SPAILKP I APG +ILAA S     SV  F   +GTSMA P ++G+VA
Sbjct: 506 ATFSSRGPNSISPAILKPDIGAPGVSILAATSPDSNSSVGGFDILAGTSMAAPVVAGVVA 565

Query: 481 LLKSVHPTWSPAAIKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGGLV 540
           LLK++HP WSPAA +SA++TT        + R  P G  I A+GS  K+ADPFDYGGG+V
Sbjct: 566 LLKALHPNWSPAAFRSAIVTT--------AWRTDPFGEQIFAEGSSRKVADPFDYGGGIV 625

Query: 541 NPNAAADPGLIYDLAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSD--LPSIT 600
           NP  AADPGLIYD+   DY+  YLC  GY  S I+QL+   T C + + SV D  LPSIT
Sbjct: 626 NPEKAADPGLIYDMGPRDYI-LYLCSAGYNDSSITQLVGNVTVCSTPKTSVLDVNLPSIT 685

Query: 601 VPALRNSTTVTRTVTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKILFKVTIS 628
           +P L++  T+TRTVTNVG   SVY+ V+  P G +V V P+  VFN + K + F V +S
Sbjct: 686 IPDLKDEVTLTRTVTNVGTVDSVYKVVVEPPLGIQVVVAPETLVFNSKTKNVSFTVRVS 733

BLAST of MC10g0682 vs. NCBI nr
Match: XP_022159381.1 (subtilisin-like protease SBT3.4, partial [Momordica charantia])

HSP 1 Score: 742 bits (1915), Expect = 4.72e-262
Identity = 425/621 (68.44%), Postives = 467/621 (75.20%), Query Frame = 0

Query: 8   MAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDSGIWPDSE 67
           MA VVRVLPNSLYKMQTTRSWDFLGLSTS SQSSNLLHR KMGE VIIGV+DSGIWP+SE
Sbjct: 1   MAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGEHVIIGVLDSGIWPESE 60

Query: 68  AFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIADFGREALANEYLSAR 127
           AF DEG+GPVPSRWKGKC  G EFNSTNCNRKIIGARWYNKG IAD G+ ALANEY SAR
Sbjct: 61  AFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKGXIADXGQXALANEYXSAR 120

Query: 128 DLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALWSATGIGSTADILK 187
           D  GHGTH ASTAA S VP+V+Y GLGAGTVRGGAPRA LAIYK LWS  G GS ADIL 
Sbjct: 121 DFXGHGTHXASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAIYKVLWSE-GAGSGADILM 180

Query: 188 AIDEAIHNGVDVLSLSLA-GGLPIFPEFSEESGVAIGSFHAIARGISVVCAGGNDGPNKQ 247
           A+D+AIH+GVDVLSLSL  GG+PI PEFSE+S VAIG+FHA ARGISVVCAGGNDGP+KQ
Sbjct: 181 AMDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGAFHATARGISVVCAGGNDGPDKQ 240

Query: 248 TIVNTAPWIFTVAASTMDRAFLSSITLGDNANYLGDFELSYLLYECIISKALV------- 307
           TIVNTAPWIFTVAAST+DRAFL+ +TLGDN  +LG    S+   E  I   LV       
Sbjct: 241 TIVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQ---SFFAGENDIVGKLVCSTRRRL 300

Query: 308 ----EYLGN---IRENQRLSFSVKYLHIHLLRRAEFVAAMFPMSRPSLSFGTVTISQRTK 367
               + LGN   +  N  L F+ K + + + ++A  V A+    R +   G +   Q   
Sbjct: 301 CSCKDILGNDTSLSGNIVLCFN-KLVDLEMTQKA--VVAV----RQANGNGIIVAGQHDD 360

Query: 368 DISG--------VYQIYIHWAYIRYTLVIHGEG---GARNNVVGKHMSTNIAVFSSRGPN 427
            +          V    +   +  Y L         G    +VGK +STN+A FSSRGPN
Sbjct: 361 ILFPCGADFPCIVLDPDVGTKFFHYXLDTSNPMVRIGRAXTIVGKPISTNVAFFSSRGPN 420

Query: 428 SASPAILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGIVALLKSVHPTW 487
           SASPAILKP IAAPGSNILAAIS  + FS K FAF SGTSMA PHISGIVALLKS+HPTW
Sbjct: 421 SASPAILKPDIAAPGSNILAAISPRNPFSDKGFAFQSGTSMAAPHISGIVALLKSLHPTW 480

Query: 488 SPAAIKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGGLVNPNAAADPG 547
           SPAAI+SA+ TT        +  K P GAPILA+ SPPKLADPFDYGGGLVNPNAAADPG
Sbjct: 481 SPAAIRSAJSTT--------ARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADPG 540

Query: 548 LIYDLAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSDL--PSITVPALRNSTT 598
           LIYDLAI DYVKYYLCGMGYK S ISQL +QKTECPSQR SV DL  PSITVPALRNSTT
Sbjct: 541 LIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRSSVLDLNLPSITVPALRNSTT 600

BLAST of MC10g0682 vs. NCBI nr
Match: XP_022931496.1 (subtilisin-like protease SBT3.7 isoform X4 [Cucurbita moschata])

HSP 1 Score: 669 bits (1726), Expect = 4.27e-231
Identity = 372/646 (57.59%), Postives = 456/646 (70.59%), Query Frame = 0

Query: 1   QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDS 60
           Q  + + M +V RV P+SLYKM TTRSWDFLGLS+S S SSNLLHR+KMG++VIIGV+DS
Sbjct: 90  QAQKLARMPIVARVFPSSLYKMHTTRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDS 149

Query: 61  GIWPDSEAFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIADFGREALA 120
           G WP+SE+F D+G+GP+PSRWKG C  G +FNS +CN+K+IGARW+ +  IAD G EA+ 
Sbjct: 150 GFWPESESFNDKGMGPIPSRWKGICQLGEDFNSFHCNKKVIGARWFARALIADHGEEAVF 209

Query: 121 NEYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALWSATGIG 180
            +YLSARD  GHGTH ASTA  + V +VSY G   GT+RGGAP ARLAIYK +WS   +G
Sbjct: 210 KDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNRRGTLRGGAPLARLAIYKVIWSDRRLG 269

Query: 181 STADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISVVCAGGN 240
           S ADILK IDEAIH+GVDVLS+S+ G +P+ P+ SE + VAIGSFHAIARG+SVVCAGGN
Sbjct: 270 SAADILKGIDEAIHDGVDVLSMSIGGSIPLPPDVSEWNTVAIGSFHAIARGVSVVCAGGN 329

Query: 241 DGPNKQTIVNTAPWIFTVAASTMDRAFLSSIT-LGDNANYLGDFELSYLLYECIISKALV 300
           +G  +QT+VN APW+FTVAAST+DRAFL+SIT LGDNA YLG       L + ++ K +V
Sbjct: 330 EGSIQQTVVNVAPWLFTVAASTIDRAFLTSITTLGDNATYLGQ----TFLKKDVVGKLVV 389

Query: 301 ---EYLGNIRENQRLSFSVKYL-HIHLLRRAEFVAAMFPMSRPSLSFGTVTISQRTKDIS 360
                 G++  +     +V  L  I L  +A     + P+ +  +  G +   Q T DI 
Sbjct: 390 MDRRCAGHLGSDIPTGGNVVLLCFIDLAMKAAASNPVMPVKQAKV-VGVIYAGQHT-DIL 449

Query: 361 G-----VYQIYIHWAYIRYTLVIHGEGG-----ARNNVVGKHMSTNIAVFSSRGPNSASP 420
           G        +  H      T ++  E       A   +VGK +S+ IA FSSRGPNS SP
Sbjct: 450 GPCDVPCIHVDTHVGTKLLTYILSDEKALIRLKATRTIVGKPISSRIAYFSSRGPNSVSP 509

Query: 421 AILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGIVALLKSVHPTWSPAA 480
            ILKP IAAPGSNI+AA+  +H    K FA  SGTSMATPHISGIVAL+KS+ PTWSPAA
Sbjct: 510 GILKPDIAAPGSNIIAAVPPNHKGGDKGFAAMSGTSMATPHISGIVALIKSLRPTWSPAA 569

Query: 481 IKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGGLVNPNAAADPGLIYD 540
           IKSALITT        +  + P G PI A+GSPPK+ADPFDYGGG+V+ NAA DPGLIYD
Sbjct: 570 IKSALITT--------ARVEDPSGLPIFAEGSPPKVADPFDYGGGVVDANAAVDPGLIYD 629

Query: 541 LAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSDL--PSITVPALRNSTTVTRT 600
           L   DY+ YY+C MGY  + IS L QQK  CPS+R SV DL  P+ITVPAL NSTTVTRT
Sbjct: 630 LGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRASVLDLNLPTITVPALTNSTTVTRT 689

Query: 601 VTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKILFKVTIS 627
           VTNVGN ++VY+AVI+AP G++V V P+V VFN  VKKI FKV  S
Sbjct: 690 VTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNVKKISFKVKFS 721

BLAST of MC10g0682 vs. NCBI nr
Match: XP_022931497.1 (subtilisin-like protease SBT3.7 isoform X5 [Cucurbita moschata])

HSP 1 Score: 669 bits (1726), Expect = 4.27e-231
Identity = 372/646 (57.59%), Postives = 456/646 (70.59%), Query Frame = 0

Query: 1   QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDS 60
           Q  + + M +V RV P+SLYKM TTRSWDFLGLS+S S SSNLLHR+KMG++VIIGV+DS
Sbjct: 90  QAQKLARMPIVARVFPSSLYKMHTTRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDS 149

Query: 61  GIWPDSEAFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIADFGREALA 120
           G WP+SE+F D+G+GP+PSRWKG C  G +FNS +CN+K+IGARW+ +  IAD G EA+ 
Sbjct: 150 GFWPESESFNDKGMGPIPSRWKGICQLGEDFNSFHCNKKVIGARWFARALIADHGEEAVF 209

Query: 121 NEYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALWSATGIG 180
            +YLSARD  GHGTH ASTA  + V +VSY G   GT+RGGAP ARLAIYK +WS   +G
Sbjct: 210 KDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNRRGTLRGGAPLARLAIYKVIWSDRRLG 269

Query: 181 STADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISVVCAGGN 240
           S ADILK IDEAIH+GVDVLS+S+ G +P+ P+ SE + VAIGSFHAIARG+SVVCAGGN
Sbjct: 270 SAADILKGIDEAIHDGVDVLSMSIGGSIPLPPDVSEWNTVAIGSFHAIARGVSVVCAGGN 329

Query: 241 DGPNKQTIVNTAPWIFTVAASTMDRAFLSSIT-LGDNANYLGDFELSYLLYECIISKALV 300
           +G  +QT+VN APW+FTVAAST+DRAFL+SIT LGDNA YLG       L + ++ K +V
Sbjct: 330 EGSIQQTVVNVAPWLFTVAASTIDRAFLTSITTLGDNATYLGQ----TFLKKDVVGKLVV 389

Query: 301 ---EYLGNIRENQRLSFSVKYL-HIHLLRRAEFVAAMFPMSRPSLSFGTVTISQRTKDIS 360
                 G++  +     +V  L  I L  +A     + P+ +  +  G +   Q T DI 
Sbjct: 390 MDRRCAGHLGSDIPTGGNVVLLCFIDLAMKAAASNPVMPVKQAKV-VGVIYAGQHT-DIL 449

Query: 361 G-----VYQIYIHWAYIRYTLVIHGEGG-----ARNNVVGKHMSTNIAVFSSRGPNSASP 420
           G        +  H      T ++  E       A   +VGK +S+ IA FSSRGPNS SP
Sbjct: 450 GPCDVPCIHVDTHVGTKLLTYILSDEKALIRLKATRTIVGKPISSRIAYFSSRGPNSVSP 509

Query: 421 AILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGIVALLKSVHPTWSPAA 480
            ILKP IAAPGSNI+AA+  +H    K FA  SGTSMATPHISGIVAL+KS+ PTWSPAA
Sbjct: 510 GILKPDIAAPGSNIIAAVPPNHKGGDKGFAAMSGTSMATPHISGIVALIKSLRPTWSPAA 569

Query: 481 IKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGGLVNPNAAADPGLIYD 540
           IKSALITT        +  + P G PI A+GSPPK+ADPFDYGGG+V+ NAA DPGLIYD
Sbjct: 570 IKSALITT--------ARVEDPSGLPIFAEGSPPKVADPFDYGGGVVDANAAVDPGLIYD 629

Query: 541 LAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSDL--PSITVPALRNSTTVTRT 600
           L   DY+ YY+C MGY  + IS L QQK  CPS+R SV DL  P+ITVPAL NSTTVTRT
Sbjct: 630 LGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRASVLDLNLPTITVPALTNSTTVTRT 689

Query: 601 VTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKILFKVTIS 627
           VTNVGN ++VY+AVI+AP G++V V P+V VFN  VKKI FKV  S
Sbjct: 690 VTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNVKKISFKVKFS 721

BLAST of MC10g0682 vs. NCBI nr
Match: XP_022931495.1 (subtilisin-like protease SBT3.7 isoform X3 [Cucurbita moschata])

HSP 1 Score: 669 bits (1726), Expect = 4.57e-231
Identity = 372/646 (57.59%), Postives = 456/646 (70.59%), Query Frame = 0

Query: 1   QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDS 60
           Q  + + M +V RV P+SLYKM TTRSWDFLGLS+S S SSNLLHR+KMG++VIIGV+DS
Sbjct: 92  QAQKLARMPIVARVFPSSLYKMHTTRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDS 151

Query: 61  GIWPDSEAFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIADFGREALA 120
           G WP+SE+F D+G+GP+PSRWKG C  G +FNS +CN+K+IGARW+ +  IAD G EA+ 
Sbjct: 152 GFWPESESFNDKGMGPIPSRWKGICQLGEDFNSFHCNKKVIGARWFARALIADHGEEAVF 211

Query: 121 NEYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALWSATGIG 180
            +YLSARD  GHGTH ASTA  + V +VSY G   GT+RGGAP ARLAIYK +WS   +G
Sbjct: 212 KDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNRRGTLRGGAPLARLAIYKVIWSDRRLG 271

Query: 181 STADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISVVCAGGN 240
           S ADILK IDEAIH+GVDVLS+S+ G +P+ P+ SE + VAIGSFHAIARG+SVVCAGGN
Sbjct: 272 SAADILKGIDEAIHDGVDVLSMSIGGSIPLPPDVSEWNTVAIGSFHAIARGVSVVCAGGN 331

Query: 241 DGPNKQTIVNTAPWIFTVAASTMDRAFLSSIT-LGDNANYLGDFELSYLLYECIISKALV 300
           +G  +QT+VN APW+FTVAAST+DRAFL+SIT LGDNA YLG       L + ++ K +V
Sbjct: 332 EGSIQQTVVNVAPWLFTVAASTIDRAFLTSITTLGDNATYLGQ----TFLKKDVVGKLVV 391

Query: 301 ---EYLGNIRENQRLSFSVKYL-HIHLLRRAEFVAAMFPMSRPSLSFGTVTISQRTKDIS 360
                 G++  +     +V  L  I L  +A     + P+ +  +  G +   Q T DI 
Sbjct: 392 MDRRCAGHLGSDIPTGGNVVLLCFIDLAMKAAASNPVMPVKQAKV-VGVIYAGQHT-DIL 451

Query: 361 G-----VYQIYIHWAYIRYTLVIHGEGG-----ARNNVVGKHMSTNIAVFSSRGPNSASP 420
           G        +  H      T ++  E       A   +VGK +S+ IA FSSRGPNS SP
Sbjct: 452 GPCDVPCIHVDTHVGTKLLTYILSDEKALIRLKATRTIVGKPISSRIAYFSSRGPNSVSP 511

Query: 421 AILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGIVALLKSVHPTWSPAA 480
            ILKP IAAPGSNI+AA+  +H    K FA  SGTSMATPHISGIVAL+KS+ PTWSPAA
Sbjct: 512 GILKPDIAAPGSNIIAAVPPNHKGGDKGFAAMSGTSMATPHISGIVALIKSLRPTWSPAA 571

Query: 481 IKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGGLVNPNAAADPGLIYD 540
           IKSALITT        +  + P G PI A+GSPPK+ADPFDYGGG+V+ NAA DPGLIYD
Sbjct: 572 IKSALITT--------ARVEDPSGLPIFAEGSPPKVADPFDYGGGVVDANAAVDPGLIYD 631

Query: 541 LAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSDL--PSITVPALRNSTTVTRT 600
           L   DY+ YY+C MGY  + IS L QQK  CPS+R SV DL  P+ITVPAL NSTTVTRT
Sbjct: 632 LGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRASVLDLNLPTITVPALTNSTTVTRT 691

Query: 601 VTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKILFKVTIS 627
           VTNVGN ++VY+AVI+AP G++V V P+V VFN  VKKI FKV  S
Sbjct: 692 VTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNVKKISFKVKFS 723

BLAST of MC10g0682 vs. NCBI nr
Match: XP_016901145.1 (PREDICTED: subtilisin-like protease SBT3.7 isoform X2 [Cucumis melo])

HSP 1 Score: 666 bits (1719), Expect = 5.25e-231
Identity = 373/644 (57.92%), Postives = 451/644 (70.03%), Query Frame = 0

Query: 6   SEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDSGIWPD 65
           SEM+ VVRV+P+SLYK+ TTRSWDFLGLS+S S+SSNLLHR+K G+DVIIGV+DSGIWP+
Sbjct: 25  SEMSRVVRVVPSSLYKVHTTRSWDFLGLSSSPSESSNLLHRAK-GDDVIIGVIDSGIWPE 84

Query: 66  SEAFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIADFGREALANEYLS 125
           SE+F D+GLGP+PSRWKG C  G +FNSTNCN+KIIGARW+ K F+AD+GREALA EYLS
Sbjct: 85  SESFKDKGLGPIPSRWKGTCESGEQFNSTNCNKKIIGARWFVKAFVADYGREALAKEYLS 144

Query: 126 ARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALWSATGIGSTADI 185
            RDL GHGTH ASTAA S V +++Y    AGT RGGAP ARLAIYKALW+  G+GS+ADI
Sbjct: 145 PRDLHGHGTHTASTAAGSFVANINYHNNAAGTARGGAPLARLAIYKALWTNRGVGSSADI 204

Query: 186 LKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISVVCAGGNDGPNK 245
           LKAIDEAIH+GVDVLS+S+ G  P +PEF+E S +A GSFHAI +GISVVCA GN GP+ 
Sbjct: 205 LKAIDEAIHDGVDVLSISIGGSPPFYPEFTELSDIAFGSFHAITKGISVVCAAGNSGPSP 264

Query: 246 QTIVNTAPWIFTVAASTMDRAFLSSIT-LGDNANYLGDFELSY---LLYECIISKALVEY 305
           Q + N APWIFTVA +T+DRAFLSSIT L DN  ++G   L     L+ E +  +   + 
Sbjct: 265 QMVDNVAPWIFTVATNTIDRAFLSSITTLRDNTTFMGQSLLESKKDLVAELVSWERCDQL 324

Query: 306 LGN---IRENQRLSFSVKYLHIHLLRRAEFVAAMFPMSRPSLSFGTVTISQRTKD----- 365
            GN   I     L F     +  + + AE V         +   G +   Q+  +     
Sbjct: 325 SGNEAFINGKVVLCFPKLADYSTISKAAEVVVR-------ANGTGIIVAGQQVDNNLLAC 384

Query: 366 ISGVYQIYIHWAYIRYTLVIHGEGG---------ARNNVVGKHMSTNIAVFSSRGPNSAS 425
           IS      +    +   L  +             A   ++GK ++  IA FSSRGPNS S
Sbjct: 385 ISSPIPCILVDTIVGSKLFFYFLQNSDHPVVMLRAARTIIGKPIAPTIAHFSSRGPNSVS 444

Query: 426 PAILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGIVALLKSVHPTWSPA 485
           P ILKP I+APGSNIL+A+S H+ F+ K F+  SGTSMATPH+S IVALLKSVHPTWSPA
Sbjct: 445 PPILKPDISAPGSNILSAVSPHYFFNEKGFSLMSGTSMATPHVSAIVALLKSVHPTWSPA 504

Query: 486 AIKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGGLVNPNAAADPGLIY 545
           AIKSAL+TT         T   P G PI ADG+PPK+ADPFDYG G+V+ NAA DPGLIY
Sbjct: 505 AIKSALMTTA-------RTEVSP-GLPIFADGTPPKVADPFDYGAGVVDANAAVDPGLIY 564

Query: 546 DLAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSDL--PSITVPALRNSTTVTR 605
           D+   DY+ YYLCGMGY+   IS L  +KTECP QRLS+ DL  P+IT+P+L NST VTR
Sbjct: 565 DMGRKDYIDYYLCGMGYEDKDISHLTHRKTECPLQRLSLLDLNLPAITIPSLVNSTIVTR 624

Query: 606 TVTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKILFKV 624
           TVTNVGN S VY+A I AP G +VSV PQV VFN +VKKI FKV
Sbjct: 625 TVTNVGNLSCVYKAKIEAPFGCKVSVNPQVLVFNSQVKKISFKV 652

BLAST of MC10g0682 vs. ExPASy TrEMBL
Match: A0A6J1E3R4 (subtilisin-like protease SBT3.4 OS=Momordica charantia OX=3673 GN=LOC111025797 PE=3 SV=1)

HSP 1 Score: 742 bits (1916), Expect = 1.61e-262
Identity = 426/621 (68.60%), Postives = 467/621 (75.20%), Query Frame = 0

Query: 8   MAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDSGIWPDSE 67
           MA VVRVLPNSLYKMQTTRSWDFLGLSTS SQSSNLLHR KMGE VIIGV+DSGIWP+SE
Sbjct: 1   MAGVVRVLPNSLYKMQTTRSWDFLGLSTSPSQSSNLLHRGKMGEHVIIGVLDSGIWPESE 60

Query: 68  AFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIADFGREALANEYLSAR 127
           AF DEG+GPVPSRWKGKC  G EFNSTNCNRKIIGARWYNKG IAD G+ ALANEY SAR
Sbjct: 61  AFRDEGMGPVPSRWKGKCESGEEFNSTNCNRKIIGARWYNKGXIADXGQXALANEYXSAR 120

Query: 128 DLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALWSATGIGSTADILK 187
           D  GHGTH ASTAA S VP+V+Y GLGAGTVRGGAPRA LAIYK LWS  G GS ADIL 
Sbjct: 121 DFXGHGTHXASTAAGSFVPNVNYQGLGAGTVRGGAPRACLAIYKVLWSE-GAGSGADILM 180

Query: 188 AIDEAIHNGVDVLSLSLA-GGLPIFPEFSEESGVAIGSFHAIARGISVVCAGGNDGPNKQ 247
           A+D+AIH+GVDVLSLSL  GG+PI PEFSE+S VAIG+FHA ARGISVVCAGGNDGP+KQ
Sbjct: 181 AMDDAIHDGVDVLSLSLRFGGVPISPEFSEDSVVAIGAFHATARGISVVCAGGNDGPDKQ 240

Query: 248 TIVNTAPWIFTVAASTMDRAFLSSITLGDNANYLGDFELSYLLYECIISKALV------- 307
           TIVNTAPWIFTVAAST+DRAFL+ +TLGDN  +LG    S+   E  I   LV       
Sbjct: 241 TIVNTAPWIFTVAASTIDRAFLAPVTLGDNTTHLGQ---SFFAGENDIVGKLVCSTRRRL 300

Query: 308 ----EYLGN---IRENQRLSFSVKYLHIHLLRRAEFVAAMFPMSRPSLSFGTVTISQRTK 367
               + LGN   +  N  L F+ K + + + ++A  V A+    R +   G +   Q   
Sbjct: 301 CSCKDILGNDTSLSGNIVLCFN-KLVDLEMTQKA--VVAV----RQANGNGIIVAGQHDD 360

Query: 368 DISG--------VYQIYIHWAYIRYTLVIHGEG---GARNNVVGKHMSTNIAVFSSRGPN 427
            +          V    +   +  Y L         G    +VGK +STN+A FSSRGPN
Sbjct: 361 ILFPCGADFPCIVLDPDVGTKFFHYXLDTSNPMVRIGRAXTIVGKPISTNVAFFSSRGPN 420

Query: 428 SASPAILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGIVALLKSVHPTW 487
           SASPAILKP IAAPGSNILAAIS  + FS K FAF SGTSMA PHISGIVALLKS+HPTW
Sbjct: 421 SASPAILKPDIAAPGSNILAAISPRNPFSDKGFAFQSGTSMAAPHISGIVALLKSLHPTW 480

Query: 488 SPAAIKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGGLVNPNAAADPG 547
           SPAAI+SAL TT        +  K P GAPILA+ SPPKLADPFDYGGGLVNPNAAADPG
Sbjct: 481 SPAAIRSALSTT--------ARVKGPSGAPILAESSPPKLADPFDYGGGLVNPNAAADPG 540

Query: 548 LIYDLAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSDL--PSITVPALRNSTT 598
           LIYDLAI DYVKYYLCGMGYK S ISQL +QKTECPSQR SV DL  PSITVPALRNSTT
Sbjct: 541 LIYDLAIADYVKYYLCGMGYKNSDISQLTEQKTECPSQRSSVLDLNLPSITVPALRNSTT 600

BLAST of MC10g0682 vs. ExPASy TrEMBL
Match: A0A6J1ETU3 (subtilisin-like protease SBT3.7 isoform X5 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 669 bits (1726), Expect = 2.07e-231
Identity = 372/646 (57.59%), Postives = 456/646 (70.59%), Query Frame = 0

Query: 1   QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDS 60
           Q  + + M +V RV P+SLYKM TTRSWDFLGLS+S S SSNLLHR+KMG++VIIGV+DS
Sbjct: 90  QAQKLARMPIVARVFPSSLYKMHTTRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDS 149

Query: 61  GIWPDSEAFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIADFGREALA 120
           G WP+SE+F D+G+GP+PSRWKG C  G +FNS +CN+K+IGARW+ +  IAD G EA+ 
Sbjct: 150 GFWPESESFNDKGMGPIPSRWKGICQLGEDFNSFHCNKKVIGARWFARALIADHGEEAVF 209

Query: 121 NEYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALWSATGIG 180
            +YLSARD  GHGTH ASTA  + V +VSY G   GT+RGGAP ARLAIYK +WS   +G
Sbjct: 210 KDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNRRGTLRGGAPLARLAIYKVIWSDRRLG 269

Query: 181 STADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISVVCAGGN 240
           S ADILK IDEAIH+GVDVLS+S+ G +P+ P+ SE + VAIGSFHAIARG+SVVCAGGN
Sbjct: 270 SAADILKGIDEAIHDGVDVLSMSIGGSIPLPPDVSEWNTVAIGSFHAIARGVSVVCAGGN 329

Query: 241 DGPNKQTIVNTAPWIFTVAASTMDRAFLSSIT-LGDNANYLGDFELSYLLYECIISKALV 300
           +G  +QT+VN APW+FTVAAST+DRAFL+SIT LGDNA YLG       L + ++ K +V
Sbjct: 330 EGSIQQTVVNVAPWLFTVAASTIDRAFLTSITTLGDNATYLGQ----TFLKKDVVGKLVV 389

Query: 301 ---EYLGNIRENQRLSFSVKYL-HIHLLRRAEFVAAMFPMSRPSLSFGTVTISQRTKDIS 360
                 G++  +     +V  L  I L  +A     + P+ +  +  G +   Q T DI 
Sbjct: 390 MDRRCAGHLGSDIPTGGNVVLLCFIDLAMKAAASNPVMPVKQAKV-VGVIYAGQHT-DIL 449

Query: 361 G-----VYQIYIHWAYIRYTLVIHGEGG-----ARNNVVGKHMSTNIAVFSSRGPNSASP 420
           G        +  H      T ++  E       A   +VGK +S+ IA FSSRGPNS SP
Sbjct: 450 GPCDVPCIHVDTHVGTKLLTYILSDEKALIRLKATRTIVGKPISSRIAYFSSRGPNSVSP 509

Query: 421 AILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGIVALLKSVHPTWSPAA 480
            ILKP IAAPGSNI+AA+  +H    K FA  SGTSMATPHISGIVAL+KS+ PTWSPAA
Sbjct: 510 GILKPDIAAPGSNIIAAVPPNHKGGDKGFAAMSGTSMATPHISGIVALIKSLRPTWSPAA 569

Query: 481 IKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGGLVNPNAAADPGLIYD 540
           IKSALITT        +  + P G PI A+GSPPK+ADPFDYGGG+V+ NAA DPGLIYD
Sbjct: 570 IKSALITT--------ARVEDPSGLPIFAEGSPPKVADPFDYGGGVVDANAAVDPGLIYD 629

Query: 541 LAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSDL--PSITVPALRNSTTVTRT 600
           L   DY+ YY+C MGY  + IS L QQK  CPS+R SV DL  P+ITVPAL NSTTVTRT
Sbjct: 630 LGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRASVLDLNLPTITVPALTNSTTVTRT 689

Query: 601 VTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKILFKVTIS 627
           VTNVGN ++VY+AVI+AP G++V V P+V VFN  VKKI FKV  S
Sbjct: 690 VTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNVKKISFKVKFS 721

BLAST of MC10g0682 vs. ExPASy TrEMBL
Match: A0A6J1ETT0 (subtilisin-like protease SBT3.7 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 669 bits (1726), Expect = 2.07e-231
Identity = 372/646 (57.59%), Postives = 456/646 (70.59%), Query Frame = 0

Query: 1   QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDS 60
           Q  + + M +V RV P+SLYKM TTRSWDFLGLS+S S SSNLLHR+KMG++VIIGV+DS
Sbjct: 90  QAQKLARMPIVARVFPSSLYKMHTTRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDS 149

Query: 61  GIWPDSEAFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIADFGREALA 120
           G WP+SE+F D+G+GP+PSRWKG C  G +FNS +CN+K+IGARW+ +  IAD G EA+ 
Sbjct: 150 GFWPESESFNDKGMGPIPSRWKGICQLGEDFNSFHCNKKVIGARWFARALIADHGEEAVF 209

Query: 121 NEYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALWSATGIG 180
            +YLSARD  GHGTH ASTA  + V +VSY G   GT+RGGAP ARLAIYK +WS   +G
Sbjct: 210 KDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNRRGTLRGGAPLARLAIYKVIWSDRRLG 269

Query: 181 STADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISVVCAGGN 240
           S ADILK IDEAIH+GVDVLS+S+ G +P+ P+ SE + VAIGSFHAIARG+SVVCAGGN
Sbjct: 270 SAADILKGIDEAIHDGVDVLSMSIGGSIPLPPDVSEWNTVAIGSFHAIARGVSVVCAGGN 329

Query: 241 DGPNKQTIVNTAPWIFTVAASTMDRAFLSSIT-LGDNANYLGDFELSYLLYECIISKALV 300
           +G  +QT+VN APW+FTVAAST+DRAFL+SIT LGDNA YLG       L + ++ K +V
Sbjct: 330 EGSIQQTVVNVAPWLFTVAASTIDRAFLTSITTLGDNATYLGQ----TFLKKDVVGKLVV 389

Query: 301 ---EYLGNIRENQRLSFSVKYL-HIHLLRRAEFVAAMFPMSRPSLSFGTVTISQRTKDIS 360
                 G++  +     +V  L  I L  +A     + P+ +  +  G +   Q T DI 
Sbjct: 390 MDRRCAGHLGSDIPTGGNVVLLCFIDLAMKAAASNPVMPVKQAKV-VGVIYAGQHT-DIL 449

Query: 361 G-----VYQIYIHWAYIRYTLVIHGEGG-----ARNNVVGKHMSTNIAVFSSRGPNSASP 420
           G        +  H      T ++  E       A   +VGK +S+ IA FSSRGPNS SP
Sbjct: 450 GPCDVPCIHVDTHVGTKLLTYILSDEKALIRLKATRTIVGKPISSRIAYFSSRGPNSVSP 509

Query: 421 AILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGIVALLKSVHPTWSPAA 480
            ILKP IAAPGSNI+AA+  +H    K FA  SGTSMATPHISGIVAL+KS+ PTWSPAA
Sbjct: 510 GILKPDIAAPGSNIIAAVPPNHKGGDKGFAAMSGTSMATPHISGIVALIKSLRPTWSPAA 569

Query: 481 IKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGGLVNPNAAADPGLIYD 540
           IKSALITT        +  + P G PI A+GSPPK+ADPFDYGGG+V+ NAA DPGLIYD
Sbjct: 570 IKSALITT--------ARVEDPSGLPIFAEGSPPKVADPFDYGGGVVDANAAVDPGLIYD 629

Query: 541 LAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSDL--PSITVPALRNSTTVTRT 600
           L   DY+ YY+C MGY  + IS L QQK  CPS+R SV DL  P+ITVPAL NSTTVTRT
Sbjct: 630 LGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRASVLDLNLPTITVPALTNSTTVTRT 689

Query: 601 VTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKILFKVTIS 627
           VTNVGN ++VY+AVI+AP G++V V P+V VFN  VKKI FKV  S
Sbjct: 690 VTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNVKKISFKVKFS 721

BLAST of MC10g0682 vs. ExPASy TrEMBL
Match: A0A6J1EZK4 (subtilisin-like protease SBT3.7 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 669 bits (1726), Expect = 2.21e-231
Identity = 372/646 (57.59%), Postives = 456/646 (70.59%), Query Frame = 0

Query: 1   QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDS 60
           Q  + + M +V RV P+SLYKM TTRSWDFLGLS+S S SSNLLHR+KMG++VIIGV+DS
Sbjct: 92  QAQKLARMPIVARVFPSSLYKMHTTRSWDFLGLSSSPSASSNLLHRAKMGDNVIIGVIDS 151

Query: 61  GIWPDSEAFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIADFGREALA 120
           G WP+SE+F D+G+GP+PSRWKG C  G +FNS +CN+K+IGARW+ +  IAD G EA+ 
Sbjct: 152 GFWPESESFNDKGMGPIPSRWKGICQLGEDFNSFHCNKKVIGARWFARALIADHGEEAVF 211

Query: 121 NEYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALWSATGIG 180
            +YLSARD  GHGTH ASTA  + V +VSY G   GT+RGGAP ARLAIYK +WS   +G
Sbjct: 212 KDYLSARDNKGHGTHTASTAGGAFVRNVSYFGNRRGTLRGGAPLARLAIYKVIWSDRRLG 271

Query: 181 STADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISVVCAGGN 240
           S ADILK IDEAIH+GVDVLS+S+ G +P+ P+ SE + VAIGSFHAIARG+SVVCAGGN
Sbjct: 272 SAADILKGIDEAIHDGVDVLSMSIGGSIPLPPDVSEWNTVAIGSFHAIARGVSVVCAGGN 331

Query: 241 DGPNKQTIVNTAPWIFTVAASTMDRAFLSSIT-LGDNANYLGDFELSYLLYECIISKALV 300
           +G  +QT+VN APW+FTVAAST+DRAFL+SIT LGDNA YLG       L + ++ K +V
Sbjct: 332 EGSIQQTVVNVAPWLFTVAASTIDRAFLTSITTLGDNATYLGQ----TFLKKDVVGKLVV 391

Query: 301 ---EYLGNIRENQRLSFSVKYL-HIHLLRRAEFVAAMFPMSRPSLSFGTVTISQRTKDIS 360
                 G++  +     +V  L  I L  +A     + P+ +  +  G +   Q T DI 
Sbjct: 392 MDRRCAGHLGSDIPTGGNVVLLCFIDLAMKAAASNPVMPVKQAKV-VGVIYAGQHT-DIL 451

Query: 361 G-----VYQIYIHWAYIRYTLVIHGEGG-----ARNNVVGKHMSTNIAVFSSRGPNSASP 420
           G        +  H      T ++  E       A   +VGK +S+ IA FSSRGPNS SP
Sbjct: 452 GPCDVPCIHVDTHVGTKLLTYILSDEKALIRLKATRTIVGKPISSRIAYFSSRGPNSVSP 511

Query: 421 AILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGIVALLKSVHPTWSPAA 480
            ILKP IAAPGSNI+AA+  +H    K FA  SGTSMATPHISGIVAL+KS+ PTWSPAA
Sbjct: 512 GILKPDIAAPGSNIIAAVPPNHKGGDKGFAAMSGTSMATPHISGIVALIKSLRPTWSPAA 571

Query: 481 IKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGGLVNPNAAADPGLIYD 540
           IKSALITT        +  + P G PI A+GSPPK+ADPFDYGGG+V+ NAA DPGLIYD
Sbjct: 572 IKSALITT--------ARVEDPSGLPIFAEGSPPKVADPFDYGGGVVDANAAVDPGLIYD 631

Query: 541 LAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSDL--PSITVPALRNSTTVTRT 600
           L   DY+ YY+C MGY  + IS L QQK  CPS+R SV DL  P+ITVPAL NSTTVTRT
Sbjct: 632 LGATDYIYYYMCSMGYTKAEISHLSQQKIVCPSKRASVLDLNLPTITVPALTNSTTVTRT 691

Query: 601 VTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKILFKVTIS 627
           VTNVGN ++VY+AVI+AP G++V V P+V VFN  VKKI FKV  S
Sbjct: 692 VTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVFNSNVKKISFKVKFS 723

BLAST of MC10g0682 vs. ExPASy TrEMBL
Match: A0A1S4DZI7 (subtilisin-like protease SBT3.7 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493167 PE=3 SV=1)

HSP 1 Score: 666 bits (1719), Expect = 2.54e-231
Identity = 373/644 (57.92%), Postives = 451/644 (70.03%), Query Frame = 0

Query: 6   SEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDSGIWPD 65
           SEM+ VVRV+P+SLYK+ TTRSWDFLGLS+S S+SSNLLHR+K G+DVIIGV+DSGIWP+
Sbjct: 25  SEMSRVVRVVPSSLYKVHTTRSWDFLGLSSSPSESSNLLHRAK-GDDVIIGVIDSGIWPE 84

Query: 66  SEAFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIADFGREALANEYLS 125
           SE+F D+GLGP+PSRWKG C  G +FNSTNCN+KIIGARW+ K F+AD+GREALA EYLS
Sbjct: 85  SESFKDKGLGPIPSRWKGTCESGEQFNSTNCNKKIIGARWFVKAFVADYGREALAKEYLS 144

Query: 126 ARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALWSATGIGSTADI 185
            RDL GHGTH ASTAA S V +++Y    AGT RGGAP ARLAIYKALW+  G+GS+ADI
Sbjct: 145 PRDLHGHGTHTASTAAGSFVANINYHNNAAGTARGGAPLARLAIYKALWTNRGVGSSADI 204

Query: 186 LKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISVVCAGGNDGPNK 245
           LKAIDEAIH+GVDVLS+S+ G  P +PEF+E S +A GSFHAI +GISVVCA GN GP+ 
Sbjct: 205 LKAIDEAIHDGVDVLSISIGGSPPFYPEFTELSDIAFGSFHAITKGISVVCAAGNSGPSP 264

Query: 246 QTIVNTAPWIFTVAASTMDRAFLSSIT-LGDNANYLGDFELSY---LLYECIISKALVEY 305
           Q + N APWIFTVA +T+DRAFLSSIT L DN  ++G   L     L+ E +  +   + 
Sbjct: 265 QMVDNVAPWIFTVATNTIDRAFLSSITTLRDNTTFMGQSLLESKKDLVAELVSWERCDQL 324

Query: 306 LGN---IRENQRLSFSVKYLHIHLLRRAEFVAAMFPMSRPSLSFGTVTISQRTKD----- 365
            GN   I     L F     +  + + AE V         +   G +   Q+  +     
Sbjct: 325 SGNEAFINGKVVLCFPKLADYSTISKAAEVVVR-------ANGTGIIVAGQQVDNNLLAC 384

Query: 366 ISGVYQIYIHWAYIRYTLVIHGEGG---------ARNNVVGKHMSTNIAVFSSRGPNSAS 425
           IS      +    +   L  +             A   ++GK ++  IA FSSRGPNS S
Sbjct: 385 ISSPIPCILVDTIVGSKLFFYFLQNSDHPVVMLRAARTIIGKPIAPTIAHFSSRGPNSVS 444

Query: 426 PAILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGIVALLKSVHPTWSPA 485
           P ILKP I+APGSNIL+A+S H+ F+ K F+  SGTSMATPH+S IVALLKSVHPTWSPA
Sbjct: 445 PPILKPDISAPGSNILSAVSPHYFFNEKGFSLMSGTSMATPHVSAIVALLKSVHPTWSPA 504

Query: 486 AIKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGGLVNPNAAADPGLIY 545
           AIKSAL+TT         T   P G PI ADG+PPK+ADPFDYG G+V+ NAA DPGLIY
Sbjct: 505 AIKSALMTTA-------RTEVSP-GLPIFADGTPPKVADPFDYGAGVVDANAAVDPGLIY 564

Query: 546 DLAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSDL--PSITVPALRNSTTVTR 605
           D+   DY+ YYLCGMGY+   IS L  +KTECP QRLS+ DL  P+IT+P+L NST VTR
Sbjct: 565 DMGRKDYIDYYLCGMGYEDKDISHLTHRKTECPLQRLSLLDLNLPAITIPSLVNSTIVTR 624

Query: 606 TVTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKILFKV 624
           TVTNVGN S VY+A I AP G +VSV PQV VFN +VKKI FKV
Sbjct: 625 TVTNVGNLSCVYKAKIEAPFGCKVSVNPQVLVFNSQVKKISFKV 652

BLAST of MC10g0682 vs. TAIR 10
Match: AT4G10520.1 (Subtilase family protein )

HSP 1 Score: 525.8 bits (1353), Expect = 4.8e-149
Identity = 315/657 (47.95%), Postives = 404/657 (61.49%), Query Frame = 0

Query: 1   QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDS 60
           Q  + SE+  VV+V+PN+LY+M TTR+WD+LG+S   S S  LL ++ MG +VI+GV+DS
Sbjct: 86  QAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVSPGNSDS--LLQKANMGYNVIVGVIDS 145

Query: 61  GIWPDSEAFGDEGLGPVPSRWKGKC--GAEFN-STNCNRKIIGARWYNKGFIADFG--RE 120
           G+WP+SE F D+G GP+PSRWKG C  G  FN S +CNRK+IGA+++  G +A+FG    
Sbjct: 146 GVWPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFGVVNR 205

Query: 121 ALANEYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALWSAT 180
               EYLS RD +GHGTH AST   S +P+VSY GLG GT RGGAP   +A+YKA WS  
Sbjct: 206 TQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKACWS-- 265

Query: 181 GIGSTADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISVVCA 240
           G  S AD+LKA+DEAIH+GVD+LSLSL   +P+FPE    S   +G+FHA+A+GI VV A
Sbjct: 266 GYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETEHTS---VGAFHAVAKGIPVVIA 325

Query: 241 GGNDGPNKQTIVNTAPWIFTVAASTMDRAFLSSITLGDNANYLGDFELSYLLYECIISKA 300
            GN GP  QTI N APW+ TVAA+T DR+F ++ITLG+N   LG         + I    
Sbjct: 326 AGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITILG---------QAIYGGP 385

Query: 301 LVEYLGNIRENQRLSFSVKYLHIHLLRRAE-FVAAMFPMSRPSLS----------FGTVT 360
            + ++G       LS   + L  +     E  V   F  S PS +           G + 
Sbjct: 386 ELGFVGLTYPESPLSGDCEKLSANPNSTMEGKVVLCFAASTPSNAAIAAVINAGGLGLIM 445

Query: 361 ISQRTKDISGVYQIYIHWA------------YIRYTLVIHGEGGARNNVVGKHMSTNIAV 420
               T  ++   +    W             YIR T     +  A   + G+ +ST +A 
Sbjct: 446 AKNPTHSLTPTRK--FPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSVSTKVAT 505

Query: 421 FSSRGPNSASPAILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGIVALL 480
           FSSRGPNS SPAILKP IAAPG NILAAIS +   +   FA  SGTSMATP +SG+V LL
Sbjct: 506 FSSRGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLL 565

Query: 481 KSVHPTWSPAAIKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGGLVNP 540
           KS+HP WSP+AIKSA++TT        + R  P G PI ADGS  KLADPFDYGGGL+NP
Sbjct: 566 KSLHPDWSPSAIKSAIVTT--------AWRTDPSGEPIFADGSSRKLADPFDYGGGLINP 625

Query: 541 NAAADPGLIYDLAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSD--LPSITVP 600
             A  PGLIYD+   DYV  Y+C + Y    IS+++ + T CP+ + SV D  LPSIT+P
Sbjct: 626 EKAVKPGLIYDMTTDDYV-MYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIP 685

Query: 601 ALRNSTTVTRTVTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKILFKVTIS 628
            LR   T+TRTVTNVG  +SVY+ VI  P+G  V+V P   VF+    K  F V +S
Sbjct: 686 NLRGEVTLTRTVTNVGPVNSVYKVVIDPPTGINVAVTPAELVFDYTTTKRSFTVRVS 715

BLAST of MC10g0682 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 525.0 bits (1351), Expect = 8.2e-149
Identity = 303/667 (45.43%), Postives = 415/667 (62.22%), Query Frame = 0

Query: 1   QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDS 60
           Q  + +++  VV V+P+S YK+ TTR+WD+LGLS +  +S  LLH + MGE +IIGV+D+
Sbjct: 91  QAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKS--LLHETNMGEQIIIGVIDT 150

Query: 61  GIWPDSEAFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIADFGREALA 120
           G+WP+SE F D G GPVPS WKG C  G  FNS+NCN+K+IGA+++  GF+A+       
Sbjct: 151 GVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKLIGAKYFINGFLAENESFNST 210

Query: 121 N--EYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALW---- 180
           N  +++S RDL GHGTH ++ A  S VP++SY GL  GTVRGGAPRA +A+YKA W    
Sbjct: 211 NSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAMYKACWYLDD 270

Query: 181 SATGIGSTADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISV 240
             T   S+ADILKA+DEA+H+GVDVLS+SL   +P++ E     G+  G+FHA+ +GI+V
Sbjct: 271 DDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGETDIRDGITTGAFHAVLKGITV 330

Query: 241 VCAGGNDGPNKQTIVNTAPWIFTVAASTMDRAFLSSITLGDNANYLGDFELSYLLYE--- 300
           VC+GGN GP+  T+ NTAPWI TVAA+T+DR+F + +TLG+N   LG       +Y    
Sbjct: 331 VCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLTLGNNKVILGQ-----AMYTGPG 390

Query: 301 -CIISKALVEYLGNIRENQRLSFSVKYLHIHLLRRAEFVAAMFPMSRPSLSFGTVTISQR 360
               S    E  GN   N+  S + + L  +  R  E    +   + P      ++ ++ 
Sbjct: 391 LGFTSLVYPENPGN--SNESFSGTCEELLFNSNRTMEGKVVLCFTTSP-YGGAVLSAARY 450

Query: 361 TKDISGV---------YQIY----------IHWAYIRYTLVIHGEGGA-------RNNVV 420
            K   G+         Y I           + W      L+     G+          +V
Sbjct: 451 VKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKTLV 510

Query: 421 GKHMSTNIAVFSSRGPNSASPAILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMAT 480
           G+ + T +A FSSRGPNS +PAILKP IAAPG +ILAA + +  FS + F   SGTSMA 
Sbjct: 511 GQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAA-TTNTTFSDQGFIMLSGTSMAA 570

Query: 481 PHISGIVALLKSVHPTWSPAAIKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADP 540
           P ISG+ ALLK++H  WSPAAI+SA++TT        + +  P G  I A+GSPPKLADP
Sbjct: 571 PAISGVAALLKALHRDWSPAAIRSAIVTT--------AWKTDPFGEQIFAEGSPPKLADP 630

Query: 541 FDYGGGLVNPNAAADPGLIYDLAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVS 600
           FDYGGGLVNP  +A+PGL+YD+ + DYV  Y+C +GY  + ISQL+ + T C + + SV 
Sbjct: 631 FDYGGGLVNPEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQLIGKTTVCSNPKPSVL 690

Query: 601 D--LPSITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKI 628
           D  LPSIT+P L++  T+TRTVTNVG  +SVYR  +  P G +V+V P+  VFN   KK+
Sbjct: 691 DFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNSTTKKV 737

BLAST of MC10g0682 vs. TAIR 10
Match: AT4G10550.2 (Subtilase family protein )

HSP 1 Score: 525.0 bits (1351), Expect = 8.2e-149
Identity = 303/667 (45.43%), Postives = 415/667 (62.22%), Query Frame = 0

Query: 1   QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDS 60
           Q  + +++  VV V+P+S YK+ TTR+WD+LGLS +  +S  LLH + MGE +IIGV+D+
Sbjct: 35  QAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKS--LLHETNMGEQIIIGVIDT 94

Query: 61  GIWPDSEAFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIADFGREALA 120
           G+WP+SE F D G GPVPS WKG C  G  FNS+NCN+K+IGA+++  GF+A+       
Sbjct: 95  GVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKLIGAKYFINGFLAENESFNST 154

Query: 121 N--EYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALW---- 180
           N  +++S RDL GHGTH ++ A  S VP++SY GL  GTVRGGAPRA +A+YKA W    
Sbjct: 155 NSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAMYKACWYLDD 214

Query: 181 SATGIGSTADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISV 240
             T   S+ADILKA+DEA+H+GVDVLS+SL   +P++ E     G+  G+FHA+ +GI+V
Sbjct: 215 DDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGETDIRDGITTGAFHAVLKGITV 274

Query: 241 VCAGGNDGPNKQTIVNTAPWIFTVAASTMDRAFLSSITLGDNANYLGDFELSYLLYE--- 300
           VC+GGN GP+  T+ NTAPWI TVAA+T+DR+F + +TLG+N   LG       +Y    
Sbjct: 275 VCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLTLGNNKVILGQ-----AMYTGPG 334

Query: 301 -CIISKALVEYLGNIRENQRLSFSVKYLHIHLLRRAEFVAAMFPMSRPSLSFGTVTISQR 360
               S    E  GN   N+  S + + L  +  R  E    +   + P      ++ ++ 
Sbjct: 335 LGFTSLVYPENPGN--SNESFSGTCEELLFNSNRTMEGKVVLCFTTSP-YGGAVLSAARY 394

Query: 361 TKDISGV---------YQIY----------IHWAYIRYTLVIHGEGGA-------RNNVV 420
            K   G+         Y I           + W      L+     G+          +V
Sbjct: 395 VKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKTLV 454

Query: 421 GKHMSTNIAVFSSRGPNSASPAILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMAT 480
           G+ + T +A FSSRGPNS +PAILKP IAAPG +ILAA + +  FS + F   SGTSMA 
Sbjct: 455 GQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAA-TTNTTFSDQGFIMLSGTSMAA 514

Query: 481 PHISGIVALLKSVHPTWSPAAIKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADP 540
           P ISG+ ALLK++H  WSPAAI+SA++TT        + +  P G  I A+GSPPKLADP
Sbjct: 515 PAISGVAALLKALHRDWSPAAIRSAIVTT--------AWKTDPFGEQIFAEGSPPKLADP 574

Query: 541 FDYGGGLVNPNAAADPGLIYDLAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVS 600
           FDYGGGLVNP  +A+PGL+YD+ + DYV  Y+C +GY  + ISQL+ + T C + + SV 
Sbjct: 575 FDYGGGLVNPEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQLIGKTTVCSNPKPSVL 634

Query: 601 D--LPSITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKI 628
           D  LPSIT+P L++  T+TRTVTNVG  +SVYR  +  P G +V+V P+  VFN   KK+
Sbjct: 635 DFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNSTTKKV 681

BLAST of MC10g0682 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 525.0 bits (1351), Expect = 8.2e-149
Identity = 303/667 (45.43%), Postives = 415/667 (62.22%), Query Frame = 0

Query: 1   QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDS 60
           Q  + +++  VV V+P+S YK+ TTR+WD+LGLS +  +S  LLH + MGE +IIGV+D+
Sbjct: 107 QAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKS--LLHETNMGEQIIIGVIDT 166

Query: 61  GIWPDSEAFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIADFGREALA 120
           G+WP+SE F D G GPVPS WKG C  G  FNS+NCN+K+IGA+++  GF+A+       
Sbjct: 167 GVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKLIGAKYFINGFLAENESFNST 226

Query: 121 N--EYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALW---- 180
           N  +++S RDL GHGTH ++ A  S VP++SY GL  GTVRGGAPRA +A+YKA W    
Sbjct: 227 NSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAMYKACWYLDD 286

Query: 181 SATGIGSTADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISV 240
             T   S+ADILKA+DEA+H+GVDVLS+SL   +P++ E     G+  G+FHA+ +GI+V
Sbjct: 287 DDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGETDIRDGITTGAFHAVLKGITV 346

Query: 241 VCAGGNDGPNKQTIVNTAPWIFTVAASTMDRAFLSSITLGDNANYLGDFELSYLLYE--- 300
           VC+GGN GP+  T+ NTAPWI TVAA+T+DR+F + +TLG+N   LG       +Y    
Sbjct: 347 VCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLTLGNNKVILGQ-----AMYTGPG 406

Query: 301 -CIISKALVEYLGNIRENQRLSFSVKYLHIHLLRRAEFVAAMFPMSRPSLSFGTVTISQR 360
               S    E  GN   N+  S + + L  +  R  E    +   + P      ++ ++ 
Sbjct: 407 LGFTSLVYPENPGN--SNESFSGTCEELLFNSNRTMEGKVVLCFTTSP-YGGAVLSAARY 466

Query: 361 TKDISGV---------YQIY----------IHWAYIRYTLVIHGEGGA-------RNNVV 420
            K   G+         Y I           + W      L+     G+          +V
Sbjct: 467 VKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKTLV 526

Query: 421 GKHMSTNIAVFSSRGPNSASPAILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMAT 480
           G+ + T +A FSSRGPNS +PAILKP IAAPG +ILAA + +  FS + F   SGTSMA 
Sbjct: 527 GQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAA-TTNTTFSDQGFIMLSGTSMAA 586

Query: 481 PHISGIVALLKSVHPTWSPAAIKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADP 540
           P ISG+ ALLK++H  WSPAAI+SA++TT        + +  P G  I A+GSPPKLADP
Sbjct: 587 PAISGVAALLKALHRDWSPAAIRSAIVTT--------AWKTDPFGEQIFAEGSPPKLADP 646

Query: 541 FDYGGGLVNPNAAADPGLIYDLAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVS 600
           FDYGGGLVNP  +A+PGL+YD+ + DYV  Y+C +GY  + ISQL+ + T C + + SV 
Sbjct: 647 FDYGGGLVNPEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQLIGKTTVCSNPKPSVL 706

Query: 601 D--LPSITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKI 628
           D  LPSIT+P L++  T+TRTVTNVG  +SVYR  +  P G +V+V P+  VFN   KK+
Sbjct: 707 DFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNSTTKKV 753

BLAST of MC10g0682 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 520.4 bits (1339), Expect = 2.0e-147
Identity = 307/661 (46.44%), Postives = 413/661 (62.48%), Query Frame = 0

Query: 1   QRMETSEMAVVVRVLPNSLYKMQTTRSWDFLGLSTSRSQSSNLLHRSKMGEDVIIGVVDS 60
           Q  + +++  VV V P+S Y++ TTR+WD+LGLS +  +  NLL+ + MGE+VIIG+VDS
Sbjct: 87  QAKKLADLPEVVHVTPDSFYQLDTTRTWDYLGLSVANPK--NLLNDTNMGEEVIIGIVDS 146

Query: 61  GIWPDSEAFGDEGLGPVPSRWKGKC--GAEFNSTNCNRKIIGARWYNKGFIA--DFGREA 120
           G+WP+SE F D G+GPVPS WKG C  G  F S+ CN+K+IGA+++  GF+A  +     
Sbjct: 147 GVWPESEVFNDNGIGPVPSHWKGGCVSGENFTSSQCNKKLIGAKYFINGFLATHESFNST 206

Query: 121 LANEYLSARDLSGHGTHAASTAAASLVPDVSYGGLGAGTVRGGAPRARLAIYKALWSATG 180
            + +++S RD SGHGTH A+ A  S VP +SY GL  GTVRGGAPRAR+A+YKA W    
Sbjct: 207 ESLDFISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRGGAPRARIAMYKACWYLDR 266

Query: 181 IG----STADILKAIDEAIHNGVDVLSLSLAGGLPIFPEFSEESGVAIGSFHAIARGISV 240
                 S+ADILKA+DEA+H+GVDVLSLS+    P FPE    + +A G+FHA+ +GI+V
Sbjct: 267 FDINTCSSADILKAMDEAMHDGVDVLSLSIGYRFPYFPETDVRAVIATGAFHAVLKGITV 326

Query: 241 VCAGGNDGPNKQTIVNTAPWIFTVAASTMDRAFLSSITLGDNANYLGDFELSYLLYEC-I 300
           VC+GGN GP  QT+ NTAPWI TVAA+T+DR+F + ITLG+N   LG  +  Y   E   
Sbjct: 327 VCSGGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPITLGNNKLILG--QAMYTGPELGF 386

Query: 301 ISKALVEYLGNIREN-----QRLSFSVKY-------LHIHLLRRAEFVAAMFPMSRPSLS 360
            S    E  GN  E+     + L F+  +       L      R   V++     + +  
Sbjct: 387 TSLVYPENPGNSNESFSGDCELLFFNSNHTMAGKVVLCFTTSTRYITVSSAVSYVKEAGG 446

Query: 361 FGTVTISQRTKDISGVYQIYIHWA-----------YIRYTLVIHGEGGARNNVVGKHMST 420
            G +       ++S     +   A           YIR T +   +      +VG+ + T
Sbjct: 447 LGVIVARNPGDNLSPCEDDFPCVAVDYELGTDILLYIRSTGLPVVKIQPSKTLVGQPVGT 506

Query: 421 NIAVFSSRGPNSASPAILKPYIAAPGSNILAAISRHHIFSVKEFAFDSGTSMATPHISGI 480
            +A FSSRGPNS  PAILKP IAAPG +ILAA + +  F+ + F F SGTSMA P ISG+
Sbjct: 507 KVADFSSRGPNSIEPAILKPDIAAPGVSILAATTTNKTFNDRGFIFLSGTSMAAPTISGV 566

Query: 481 VALLKSVHPTWSPAAIKSALITTELPPFLKCSTRKRPLGAPILADGSPPKLADPFDYGGG 540
           VALLK++H  WSPAAI+SA++TT        + R  P G  I A+GSP KLADPFDYGGG
Sbjct: 567 VALLKALHRDWSPAAIRSAIVTT--------AWRTDPFGEQIFAEGSPRKLADPFDYGGG 626

Query: 541 LVNPNAAADPGLIYDLAIVDYVKYYLCGMGYKTSHISQLMQQKTECPSQRLSVSD--LPS 600
           LVNP  AA PGL+YDL + DYV  Y+C +GY  + ISQL+ + T C + + SV D  LPS
Sbjct: 627 LVNPEKAAKPGLVYDLGLEDYV-LYMCSVGYNETSISQLVGKGTVCSNPKPSVLDFNLPS 686

Query: 601 ITVPALRNSTTVTRTVTNVGNSSSVYRAVIRAPSGTRVSVEPQVPVFNPEVKKILFKVTI 628
           IT+P L++  T+TRT+TNVG   SVY+ VI  P G +V+V P+  +FN   K++ FKV +
Sbjct: 687 ITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVTPETLLFNSTTKRVSFKVKV 734

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZSB06.8e-14847.95Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 S... [more]
Q8L7I21.2e-14745.43Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 S... [more]
Q9SZY32.8e-14646.44Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 S... [more]
Q9MAP51.8e-14546.74Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 S... [more]
Q9MAP74.1e-14545.83Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 S... [more]
Match NameE-valueIdentityDescription
XP_022159381.14.72e-26268.44subtilisin-like protease SBT3.4, partial [Momordica charantia][more]
XP_022931496.14.27e-23157.59subtilisin-like protease SBT3.7 isoform X4 [Cucurbita moschata][more]
XP_022931497.14.27e-23157.59subtilisin-like protease SBT3.7 isoform X5 [Cucurbita moschata][more]
XP_022931495.14.57e-23157.59subtilisin-like protease SBT3.7 isoform X3 [Cucurbita moschata][more]
XP_016901145.15.25e-23157.92PREDICTED: subtilisin-like protease SBT3.7 isoform X2 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1E3R41.61e-26268.60subtilisin-like protease SBT3.4 OS=Momordica charantia OX=3673 GN=LOC111025797 P... [more]
A0A6J1ETU32.07e-23157.59subtilisin-like protease SBT3.7 isoform X5 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1ETT02.07e-23157.59subtilisin-like protease SBT3.7 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1EZK42.21e-23157.59subtilisin-like protease SBT3.7 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A1S4DZI72.54e-23157.92subtilisin-like protease SBT3.7 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034931... [more]
Match NameE-valueIdentityDescription
AT4G10520.14.8e-14947.95Subtilase family protein [more]
AT4G10550.18.2e-14945.43Subtilase family protein [more]
AT4G10550.28.2e-14945.43Subtilase family protein [more]
AT4G10550.38.2e-14945.43Subtilase family protein [more]
AT4G10540.12.0e-14746.44Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 50..69
score: 31.77
coord: 126..139
score: 49.21
coord: 436..452
score: 58.25
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 384..529
e-value: 9.2E-48
score: 165.1
coord: 52..261
e-value: 2.5E-84
score: 285.7
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 43..517
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 564..626
e-value: 1.0E-12
score: 48.1
NoneNo IPR availableGENE3D2.60.40.2310coord: 534..627
e-value: 1.6E-21
score: 78.4
NoneNo IPR availableGENE3D3.50.30.30coord: 262..279
e-value: 2.5E-84
score: 285.7
NoneNo IPR availablePANTHERPTHR43806:SF50SUBTILISIN-LIKE PROTEASE SBT3.1-RELATEDcoord: 5..627
NoneNo IPR availablePANTHERPTHR43806PEPTIDASE S8coord: 5..627
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 27..516
score: 28.869726
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 50..472
e-value: 7.1E-44
score: 150.2
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 437..447
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 20..471
e-value: 7.49028E-124
score: 366.538

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0682.1MC10g0682.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity