MC10g0588 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0588
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionbifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase
LocationMC10: 4863796 .. 4867140 (-)
RNA-Seq ExpressionMC10g0588
SyntenyMC10g0588
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCCTCCTTCTCTCATCGCGGCTTCTCGGTACCTTTCAATCTCTGTCTCTCTGTTGCCTTCTCTTTATTCTCTACTTGGGGTCCTGTTTTCTTCTTGTTCCGGTACAGAATCAGGATACCTTCCTCTTCCAATGGCAAATTCGTTGCAGAATTTATCTAATGCATCTCATCTCGGATTTAGATACCAATTTCCGGGTGCCGATTCTGCGCTAGAACCTGCTTTTGGATCCGTTTCTTTTCCCTCCCAGCTTCGTGCCCCCTTCCTCAAGTATGCCTGCTCCTTCATCACTCGTTATCTTCTTTTGCTTTTTAATTTGAGCTTCCATTGATGAGTTCTGTTTGCTTGTTTGAACACGATTGTAATGGGTGGTGGAATGAAGTGTATTTTGTGAGTGGGATTCTTTTGCTGCGTGATCTTGGGTCGCTCTGTCATCTGTCTTGGATTATAATTGCTGGGTATGGCCTTAAGCTTTCCATTTTGATAGGTCTCAGCAGTATACGAAGAGAGTGCAGCCAGTAATGGCTGGGAGTGTTTCTGACCAAGTGATTGTGGACCTAACTTTGAGCCCAAGAGTGAATTCTGTAAAGCCTTCCAAGACGGTGGCTATAACCGATCAGGCCACGGCTCTAGTGCAAGCTGGTGTGCCTGTGATTCGGCTGGCTGCTGGTGAACCTGATTTTGACACCCCTGCTCCAATTGTAGAGGTATTAGCTTAACCCAAAAGTCCATTTTTCAATGCCCAAGCTTCTTTAGATGCAAGTATACTTTCACATTTCTACATCTGATTGTTGATCCTTTGCCTTGATGGAAAGTTAGGCAGGAATGAATGCGATTCGGGATGGCTACACTCGCTACACGCCCAATGCAGGAACATTGGAACTCCGCCAAGCAATTTGTCATAAGCTAAAAGGTTAGTTGTGTTTAGGGAGGAAGTTTTCAGAGATGTGTTTGAGTTGTATATTAGCTTCATGTTTTTATGCAAGCTCGGAGAATTCTTTCTGTTTGCAGAGGAGAATGGGATTTCGTATACACCAGACCAAATATTGGTGAGCAATGGAGCCAAACAGAGTATTCTTCAAGCAGTTATTGCTGTTTGTTCTCCAGGACATGAGGTAAAAAAATTTCCTGATCTTGTGATGGATATAGATTCATACATACTGCTCCAGCAGCTGTTTACATAGACATATGATTTAGAGGAACAATAGTTCATTATTCTAGGGGAAACCTTGATGCAATATGCCATGTATTACCACAAATATAGAGTAAAGGGATCTGATCGTTCATTTAGTGCTTTTACTGTCGTGGTGTCGGTGATAGCCTTACAAAATCTTTTTCCCCTCAGGTTGTAATTCCCGCTCCATTTTGGGTAAGCTACCCAGAAATGGCTAGGCTGGCTGATGCAACTCCGGTGATTCTTCCTACGCGTATTGAAGACAATTTTCTCCTGGATCCAAAGCTTCTTGAAGCAAAGATCACAGAGAAGTCGAGGCTGCTGATTCTTTGTTCTCCATCCAACCCAACAGGATCTGTTTACCCGAAGGAATTACTTGAAAAGATTGCTGAAATTGTTGCAAAACATCCTAGACTTCTGGTGAGGTTTCTTAACTTTGCTGAAAGTTATTAATTGGATTGTTTGATCTTAAATTATCTTTTCTTTGCAGGTGCTTTCTGACGAAATATACGAACACATAATTTATGCTCCAGCAACTCACACAAGCTTCGCATCTTTGCCTGGCATGTGGGAAAGGACTTTGACTGTTAATGGGTTTTCTAAGGTAATTGAAACTTTATTTTCTCATTTATGCTGCTACCAGGTATTGAAGTCCATATTTGATCTAAGTATTGTTGCAATACGATTGCTTAACATTTGAAGCTGTAACATGTTAGGCTTTTGCAATGACTGGTTGGCGACTTGGATATATTGCCGGTCCTAAACACTTTGTCTCTGCATGTGGAAAAATCCAGAGTCAGGTATGTTATATAACTTTAGTATTTCTCATCCTATCTCTTAAAAATTTGAGCTTGGTAATACAATTTTCAGTTGTCCTTAACTCATCTGTCACTGCATTATGCAGAGCCGTTTTGCTTGCTTTTCTTCTGACTTTGTTGCTTACATACAGATAATAGGCATTTCTCTTCAGAGAACTAAATTGTCTATCTCTAGTCCGAGTAGTCTTTTATAGCTTTTATTCTTTGTTTTTTTTTCCATAGAAAAGGGGAAATCTAGGAATTTTGTTTTGTTTTTTTTTGTCTAATCCACGTGTAATAGGTGATGTAAAATGAATCTACCTTTGATCTTCAGTATGTTAGAATAAATTGAGGTAGATCCTTTTACTTGGGCTATTAGTTTCACTTTCTCTTTCTGCATGCAATTCGTAGAGGGACTTAACTTCTTTGATTAAGTTTCCAATTGATAATTGTCAACATTTTCTGTCTCTAGCTTATTCTGTATATTTGTTATGATAGTCCACTTCGGGAGCCAGTAGCATATCCCAAAAAGCTGGTGTTGCAGCTCTAGGAATGGGCTATGCTGGTGGGGAAGCAGTTGCCACAATGGTGAAAGCATTCCGGGAACGTCGGGATTCTTTGGTCAAAAGCTTTGGTGAAATTCCCGGTGTTAAGATTTCAGAACCCCAGGTGCCAACTTCAAACTTTTGCTTGTTTTCGAGCGCTCCTTGCTGAAATCCCTTCTTGGTCTTATATCTCATAATTGTTCTGTTAGTGTAATATCTGAAGATTCTATAACTTCTTGTAGGGAGCTTTCTACCTCTTCCTGGATTTCAGCTCTTACTATGGATCTGAAGTTGAAGGGTTTGGTGTAATTAACAATTCAGAGTCACTTTGCAGATATCTGCTTGAGAAGGGCCAGGTAAAGTTCATCCGAAAACGTCACATTTTTCATTATTATTTTCGTATTTGGAGATTGAATCAATCCAAAGCTTCATTATGATTCTTGATGAAACTTGTATAACTGCCTTTTTCCTTCTATCATTTTTGTTTCTAAACTCAAATATTGAGTAGAATTTCAAAGTCACTTCATATCTTCATAAAAGGCGAATATATGGCTGTTGATTTTTCAGGTTGCTCTAGTTCCGGGGGATGCGTTCGGTGACGACAGTTGCATCCGCATTTCGTACGCAGAATCCCTCAGTACACTGCAGGCTGCTGTGGAAAGAATTAAGAATGCAATTGAGGCAGTTAGGCCTGTTGTTCCTGTTTAAAAACTGCTAATGTATGGATAATGCAAGCCCTTCTCAATTGTTTGTTTCTATTGAGATTGATAATATTATTGCAGAAATGTGTATTCTAGTGTTTGTTCTGAAATAAATAAAACAATTCAGGG

mRNA sequence

CCTCCTCCTTCTCTCATCGCGGCTTCTCGGTACCTTTCAATCTCTGTCTCTCTGTTGCCTTCTCTTTATTCTCTACTTGGGGTCCTGTTTTCTTCTTGTTCCGGTACAGAATCAGGATACCTTCCTCTTCCAATGGCAAATTCGTTGCAGAATTTATCTAATGCATCTCATCTCGGATTTAGATACCAATTTCCGGGTGCCGATTCTGCGCTAGAACCTGCTTTTGGATCCGTTTCTTTTCCCTCCCAGCTTCGTGCCCCCTTCCTCAAGTCTCAGCAGTATACGAAGAGAGTGCAGCCAGTAATGGCTGGGAGTGTTTCTGACCAAGTGATTGTGGACCTAACTTTGAGCCCAAGAGTGAATTCTGTAAAGCCTTCCAAGACGGTGGCTATAACCGATCAGGCCACGGCTCTAGTGCAAGCTGGTGTGCCTGTGATTCGGCTGGCTGCTGGTGAACCTGATTTTGACACCCCTGCTCCAATTGTAGAGGCAGGAATGAATGCGATTCGGGATGGCTACACTCGCTACACGCCCAATGCAGGAACATTGGAACTCCGCCAAGCAATTTGTCATAAGCTAAAAGAGGAGAATGGGATTTCGTATACACCAGACCAAATATTGGTGAGCAATGGAGCCAAACAGAGTATTCTTCAAGCAGTTATTGCTGTTTGTTCTCCAGGACATGAGGTTGTAATTCCCGCTCCATTTTGGGTAAGCTACCCAGAAATGGCTAGGCTGGCTGATGCAACTCCGGTGATTCTTCCTACGCGTATTGAAGACAATTTTCTCCTGGATCCAAAGCTTCTTGAAGCAAAGATCACAGAGAAGTCGAGGCTGCTGATTCTTTGTTCTCCATCCAACCCAACAGGATCTGTTTACCCGAAGGAATTACTTGAAAAGATTGCTGAAATTGTTGCAAAACATCCTAGACTTCTGGTGCTTTCTGACGAAATATACGAACACATAATTTATGCTCCAGCAACTCACACAAGCTTCGCATCTTTGCCTGGCATGTGGGAAAGGACTTTGACTGTTAATGGGTTTTCTAAGGCTTTTGCAATGACTGGTTGGCGACTTGGATATATTGCCGGTCCTAAACACTTTGTCTCTGCATGTGGAAAAATCCAGAGTCAGTCCACTTCGGGAGCCAGTAGCATATCCCAAAAAGCTGGTGTTGCAGCTCTAGGAATGGGCTATGCTGGTGGGGAAGCAGTTGCCACAATGGTGAAAGCATTCCGGGAACGTCGGGATTCTTTGGTCAAAAGCTTTGGTGAAATTCCCGGTGTTAAGATTTCAGAACCCCAGGGAGCTTTCTACCTCTTCCTGGATTTCAGCTCTTACTATGGATCTGAAGTTGAAGGGTTTGGTGTAATTAACAATTCAGAGTCACTTTGCAGATATCTGCTTGAGAAGGGCCAGGTTGCTCTAGTTCCGGGGGATGCGTTCGGTGACGACAGTTGCATCCGCATTTCGTACGCAGAATCCCTCAGTACACTGCAGGCTGCTGTGGAAAGAATTAAGAATGCAATTGAGGCAGTTAGGCCTGTTGTTCCTGTTTAAAAACTGCTAATGTATGGATAATGCAAGCCCTTCTCAATTGTTTGTTTCTATTGAGATTGATAATATTATTGCAGAAATGTGTATTCTAGTGTTTGTTCTGAAATAAATAAAACAATTCAGGG

Coding sequence (CDS)

CCTCCTCCTTCTCTCATCGCGGCTTCTCGGTACCTTTCAATCTCTGTCTCTCTGTTGCCTTCTCTTTATTCTCTACTTGGGGTCCTGTTTTCTTCTTGTTCCGGTACAGAATCAGGATACCTTCCTCTTCCAATGGCAAATTCGTTGCAGAATTTATCTAATGCATCTCATCTCGGATTTAGATACCAATTTCCGGGTGCCGATTCTGCGCTAGAACCTGCTTTTGGATCCGTTTCTTTTCCCTCCCAGCTTCGTGCCCCCTTCCTCAAGTCTCAGCAGTATACGAAGAGAGTGCAGCCAGTAATGGCTGGGAGTGTTTCTGACCAAGTGATTGTGGACCTAACTTTGAGCCCAAGAGTGAATTCTGTAAAGCCTTCCAAGACGGTGGCTATAACCGATCAGGCCACGGCTCTAGTGCAAGCTGGTGTGCCTGTGATTCGGCTGGCTGCTGGTGAACCTGATTTTGACACCCCTGCTCCAATTGTAGAGGCAGGAATGAATGCGATTCGGGATGGCTACACTCGCTACACGCCCAATGCAGGAACATTGGAACTCCGCCAAGCAATTTGTCATAAGCTAAAAGAGGAGAATGGGATTTCGTATACACCAGACCAAATATTGGTGAGCAATGGAGCCAAACAGAGTATTCTTCAAGCAGTTATTGCTGTTTGTTCTCCAGGACATGAGGTTGTAATTCCCGCTCCATTTTGGGTAAGCTACCCAGAAATGGCTAGGCTGGCTGATGCAACTCCGGTGATTCTTCCTACGCGTATTGAAGACAATTTTCTCCTGGATCCAAAGCTTCTTGAAGCAAAGATCACAGAGAAGTCGAGGCTGCTGATTCTTTGTTCTCCATCCAACCCAACAGGATCTGTTTACCCGAAGGAATTACTTGAAAAGATTGCTGAAATTGTTGCAAAACATCCTAGACTTCTGGTGCTTTCTGACGAAATATACGAACACATAATTTATGCTCCAGCAACTCACACAAGCTTCGCATCTTTGCCTGGCATGTGGGAAAGGACTTTGACTGTTAATGGGTTTTCTAAGGCTTTTGCAATGACTGGTTGGCGACTTGGATATATTGCCGGTCCTAAACACTTTGTCTCTGCATGTGGAAAAATCCAGAGTCAGTCCACTTCGGGAGCCAGTAGCATATCCCAAAAAGCTGGTGTTGCAGCTCTAGGAATGGGCTATGCTGGTGGGGAAGCAGTTGCCACAATGGTGAAAGCATTCCGGGAACGTCGGGATTCTTTGGTCAAAAGCTTTGGTGAAATTCCCGGTGTTAAGATTTCAGAACCCCAGGGAGCTTTCTACCTCTTCCTGGATTTCAGCTCTTACTATGGATCTGAAGTTGAAGGGTTTGGTGTAATTAACAATTCAGAGTCACTTTGCAGATATCTGCTTGAGAAGGGCCAGGTTGCTCTAGTTCCGGGGGATGCGTTCGGTGACGACAGTTGCATCCGCATTTCGTACGCAGAATCCCTCAGTACACTGCAGGCTGCTGTGGAAAGAATTAAGAATGCAATTGAGGCAGTTAGGCCTGTTGTTCCTGTTTAA

Protein sequence

PPPSLIAASRYLSISVSLLPSLYSLLGVLFSSCSGTESGYLPLPMANSLQNLSNASHLGFRYQFPGADSALEPAFGSVSFPSQLRAPFLKSQQYTKRVQPVMAGSVSDQVIVDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEAGMNAIRDGYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVCSPGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCSPSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLTVNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEAVATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESLCRYLLEKGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV
Homology
BLAST of MC10g0588 vs. ExPASy Swiss-Prot
Match: E9L7A5 (Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase OS=Petunia hybrida OX=4102 GN=PPA-AT PE=1 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 2.6e-209
Identity = 377/474 (79.54%), Postives = 421/474 (88.82%), Query Frame = 0

Query: 53  SNASHLGF-RYQFPGADS-ALEPAFGSVSFPSQLRAPFLKS-----QQYTKRVQPVMAGS 112
           S++S + + R+  PG  S +L P   S+SF S L    LKS     Q Y++R   V+   
Sbjct: 8   SSSSRIAYSRHNIPGLHSDSLNPK--SISFSSNLHTFSLKSSGSRRQLYSRRTGAVVIMQ 67

Query: 113 VSDQVIVDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEAG 172
             D+V VD++LSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEAG
Sbjct: 68  SMDKVEVDISLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEAG 127

Query: 173 MNAIRDGYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVCS 232
           +NAIR+G+TRYTPNAGT+ELR AI HKLKEENG+SYTPDQILVSNGAKQSI+QAV+AVCS
Sbjct: 128 INAIREGHTRYTPNAGTMELRSAISHKLKEENGLSYTPDQILVSNGAKQSIIQAVLAVCS 187

Query: 233 PGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCSP 292
           PG EV+IPAP+WVSYPEMARLADATPVILPT I ++FLLDPKLLE+K+TEKSRLLILCSP
Sbjct: 188 PGDEVLIPAPYWVSYPEMARLADATPVILPTSISEDFLLDPKLLESKLTEKSRLLILCSP 247

Query: 293 SNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLTV 352
           SNPTGSVYP++LLE+IAEIVA+HPRLLV+SDEIYEHIIYAPATHTSFASLPGMW+RTLTV
Sbjct: 248 SNPTGSVYPRKLLEQIAEIVARHPRLLVISDEIYEHIIYAPATHTSFASLPGMWDRTLTV 307

Query: 353 NGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEAV 412
           NGFSKAFAMTGWRLGYIAGPKHF++AC KIQSQ TSGASSISQKA VAALG+GYAGGE V
Sbjct: 308 NGFSKAFAMTGWRLGYIAGPKHFIAACNKIQSQFTSGASSISQKAAVAALGLGYAGGELV 367

Query: 413 ATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESLC 472
           ATMVK+FRERRD LVKSFGEI GVKISEP+GAFYLF+D SSYYG EV+GFG INNSESLC
Sbjct: 368 ATMVKSFRERRDYLVKSFGEIEGVKISEPRGAFYLFIDLSSYYGVEVDGFGSINNSESLC 427

Query: 473 RYLLEKGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV 520
           RYLL+K QVALVPGDAFGDD+CIRISYA SLSTLQAAVERIK A+  ++P VPV
Sbjct: 428 RYLLDKAQVALVPGDAFGDDTCIRISYAASLSTLQAAVERIKKALVTIKPPVPV 479

BLAST of MC10g0588 vs. ExPASy Swiss-Prot
Match: Q9SIE1 (Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase OS=Arabidopsis thaliana OX=3702 GN=PAT PE=1 SV=2)

HSP 1 Score: 649.0 bits (1673), Expect = 4.4e-185
Identity = 334/450 (74.22%), Postives = 381/450 (84.67%), Query Frame = 0

Query: 76  GSVSFPSQLRAPF--LKSQQYTKRV----QPVMAGSVSDQVIVDLTLSPRVNSVKPSKTV 135
           GSV F   LR  F   KS   + R+    +P  A ++S    VD++LSPRV S+KPSKT+
Sbjct: 28  GSVRFQQPLRLSFSYCKSGNMSSRICAMAKPNDAETLSSS--VDMSLSPRVQSLKPSKTM 87

Query: 136 AITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEAGMNAIRDGYTRYTPNAGTLELRQAI 195
            ITD A  LVQ+GVPVIRLAAGEPDFDTP  + EAG+NAIR+G+TRYT NAG  ELR+AI
Sbjct: 88  VITDLAATLVQSGVPVIRLAAGEPDFDTPKVVAEAGINAIREGFTRYTLNAGITELREAI 147

Query: 196 CHKLKEENGISYTPDQILVSNGAKQSILQAVIAVCSPGHEVVIPAPFWVSYPEMARLADA 255
           C KLKEENG+SY PDQILVSNGAKQS+LQAV+AVCSPG EV+IPAP+WVSY E ARLADA
Sbjct: 148 CRKLKEENGLSYAPDQILVSNGAKQSLLQAVLAVCSPGDEVIIPAPYWVSYTEQARLADA 207

Query: 256 TPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCSPSNPTGSVYPKELLEKIAEIVAKHP 315
           TPV++PT+I +NFLLDPK LE+K+TEKSRLLILCSPSNPTGSVYPK LLE+IA I+AKHP
Sbjct: 208 TPVVIPTKISNNFLLDPKDLESKLTEKSRLLILCSPSNPTGSVYPKSLLEEIARIIAKHP 267

Query: 316 RLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLTVNGFSKAFAMTGWRLGYIAGPKHFV 375
           RLLVLSDEIYEHIIYAPATHTSFASLP M+ERTLTVNGFSKAFAMTGWRLGY+AGPKH V
Sbjct: 268 RLLVLSDEIYEHIIYAPATHTSFASLPDMYERTLTVNGFSKAFAMTGWRLGYLAGPKHIV 327

Query: 376 SACGKIQSQSTSGASSISQKAGVAALGMGYAGGEAVATMVKAFRERRDSLVKSFGEIPGV 435
           +AC K+Q Q +SGASSI+QKAGVAALG+G AGGE VA MVKA+RERRD LVKS G+I GV
Sbjct: 328 AACSKLQGQVSSGASSIAQKAGVAALGLGKAGGETVAEMVKAYRERRDFLVKSLGDIKGV 387

Query: 436 KISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESLCRYLLEKGQVALVPGDAFGDDSCIR 495
           KISEPQGAFYLF+DFS+YYGSE EGFG+IN+S SL  Y L+K QVA+VPGDAFGDDSCIR
Sbjct: 388 KISEPQGAFYLFIDFSAYYGSEAEGFGLINDSSSLALYFLDKFQVAMVPGDAFGDDSCIR 447

Query: 496 ISYAESLSTLQAAVERIKNAIEAVRPVVPV 520
           ISYA SL  LQAAVE+I+ A+E +R  V V
Sbjct: 448 ISYATSLDVLQAAVEKIRKALEPLRATVSV 475

BLAST of MC10g0588 vs. ExPASy Swiss-Prot
Match: Q5F4K8 (Aspartate aminotransferase OS=Pinus pinaster OX=71647 GN=AAT PE=1 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 8.0e-179
Identity = 311/405 (76.79%), Postives = 359/405 (88.64%), Query Frame = 0

Query: 112 VDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEAGMNAIRD 171
           +D++LSPRV ++KPSKT+AITD ATAL QAGVPVI LAAGEPDF+TP  + EAG+ AI+D
Sbjct: 80  LDISLSPRVAALKPSKTMAITDLATALKQAGVPVIGLAAGEPDFNTPDAVAEAGIKAIQD 139

Query: 172 GYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSIL-QAVIAVCSPGHEV 231
           GYTRYTPNAGT+E+R AICHKLKEENG+SYTPDQILVSNGAKQ I+  AV+AVCSPG EV
Sbjct: 140 GYTRYTPNAGTMEIRTAICHKLKEENGLSYTPDQILVSNGAKQCIMAAAVLAVCSPGDEV 199

Query: 232 VIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCSPSNPTG 291
           +IPAPFWVSY EMARLADATPVI+PT + D+FLL+P++  +K+ E SRLLILCSPSNPTG
Sbjct: 200 IIPAPFWVSYTEMARLADATPVIIPTLLSDDFLLNPEVFSSKLNENSRLLILCSPSNPTG 259

Query: 292 SVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLTVNGFSK 351
           SVYP+ELLE+IA+IVAKHP+LLVLSDEIYEHI+Y PA HTSFASLPGMWERTLTVNGFSK
Sbjct: 260 SVYPRELLEEIAKIVAKHPKLLVLSDEIYEHIMYPPAKHTSFASLPGMWERTLTVNGFSK 319

Query: 352 AFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEAVATMVK 411
           AFAMTGWRLGY+AGPKHFV+ACG+IQSQSTSGASSISQKAGVAAL +GYAG EAV+TMVK
Sbjct: 320 AFAMTGWRLGYLAGPKHFVTACGRIQSQSTSGASSISQKAGVAALALGYAGSEAVSTMVK 379

Query: 412 AFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESLCRYLLE 471
           A+RERRD LV+    + GVK+  PQGAFYLF DFSSYYG+EVE FGVIN SE+LCR+ LE
Sbjct: 380 AYRERRDFLVQRLQAMEGVKLPVPQGAFYLFPDFSSYYGTEVEDFGVINGSEALCRFFLE 439

Query: 472 KGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRP 516
           K QVALVPGDAFG+D CIRISYA SL TL+ A+  I+ ++  +RP
Sbjct: 440 KAQVALVPGDAFGNDDCIRISYAASLDTLRTAINNIEKSLLLLRP 484

BLAST of MC10g0588 vs. ExPASy Swiss-Prot
Match: Q8KDS8 (Aspartate/prephenate aminotransferase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) OX=194439 GN=CT0966 PE=1 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 3.7e-91
Identity = 180/395 (45.57%), Postives = 258/395 (65.32%), Query Frame = 0

Query: 116 LSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEAGMNAIRDGYTR 175
           LS RV S++ S+T+ IT  A  +   G  V+ L+AGEPDF TP  + EAG+ AIR G+TR
Sbjct: 10  LSRRVLSMQESQTMKITGLAKKMQAEGKDVVSLSAGEPDFPTPENVCEAGIEAIRKGFTR 69

Query: 176 YTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVCSPGHEVVIPAP 235
           YT N+G  EL++AI  KL+ +NG+ Y  D+I+VSNG KQ++    +A+C  G EV++PAP
Sbjct: 70  YTANSGIPELKKAIIRKLQRDNGLEYAEDEIIVSNGGKQALANTFLALCDEGDEVIVPAP 129

Query: 236 FWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCSPSNPTGSVYPK 295
           +WVS+PEMARLA+ATPVI+ T IE  + + P+ L A IT K+R+L+L SPSNP+G+VY +
Sbjct: 130 YWVSFPEMARLAEATPVIVETSIETGYKMTPEQLAAAITPKTRILVLNSPSNPSGAVYNE 189

Query: 296 ELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLTVNGFSKAFAMT 355
             +  + +++ +   + VLSDE+Y+ I Y      S A +P M    +  NG SK+++MT
Sbjct: 190 AEVRALMQVI-EGKEIFVLSDEMYDMICYGGVRPFSPARIPEMKPWVIVSNGTSKSYSMT 249

Query: 356 GWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEAVATMVKA-FRE 415
           GWR+GY+A PK  ++AC KIQSQ+TS A+SI+QKA VAAL     G +++    +A F +
Sbjct: 250 GWRIGYLAAPKWIINACDKIQSQTTSNANSIAQKAAVAALD----GDQSIVEQRRAEFEK 309

Query: 416 RRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESLCRYLLEKGQV 475
           RRD + +    I G++ + P+GAFY+F       G    G  V+ +S  +  YLL +  V
Sbjct: 310 RRDFMFRELNTISGIECTLPEGAFYIFPSIKGLLGKTFGG-KVMKDSTDVAEYLLTEHYV 369

Query: 476 ALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNA 510
           A VPGDAFG    +R+SYA S+  L  AV RI+ A
Sbjct: 370 ATVPGDAFGAPENLRLSYAASIEELAEAVNRIRKA 398

BLAST of MC10g0588 vs. ExPASy Swiss-Prot
Match: Q82WA8 (Aspartate/prephenate aminotransferase OS=Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298) OX=228410 GN=aatA PE=1 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 3.8e-88
Identity = 173/400 (43.25%), Postives = 250/400 (62.50%), Query Frame = 0

Query: 114 LTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEAGMNAIRDGY 173
           + LS RV ++KPS T+A+T +A  L   G  +I L AGEPDFDTP  I +A + AIR+G+
Sbjct: 1   MKLSQRVQAIKPSPTLAVTAKAARLKAEGKNIIGLGAGEPDFDTPLHIKDAAITAIRNGF 60

Query: 174 TRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVCSPGHEVVIP 233
           T+YT   GT  L+QAI  K K EN + + P +ILVS+G KQS    V+A   PG EV+IP
Sbjct: 61  TKYTAVGGTASLKQAIISKFKRENSLEFMPGEILVSSGGKQSFFNLVLATIDPGDEVIIP 120

Query: 234 APFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCSPSNPTGSVY 293
           AP+WVSYP++  +A+  PV + T IE+ F + P  LE  IT ++R+ ++ SPSNP+GSVY
Sbjct: 121 APYWVSYPDIVLIAEGKPVFIDTGIEEKFKISPDQLEKAITPRTRMFVVNSPSNPSGSVY 180

Query: 294 PKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFA-SLPGMWERTLTVNGFSKAF 353
             E L+ +  ++ K+P +L+ +D++YEHI+ +     +   + P +  RT+ +NG SKA+
Sbjct: 181 SLEELQALGAVLRKYPDILIATDDMYEHILLSGDGFVNILNACPDLKARTVVLNGVSKAY 240

Query: 354 AMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEAVATMVKAF 413
           AMTGWR+GY  GP   ++A   IQSQSTS  +SI+Q A  AAL         +  M++AF
Sbjct: 241 AMTGWRIGYCGGPAAIITAMENIQSQSTSNPNSIAQVAAEAALN---GDQSCMVPMIEAF 300

Query: 414 RERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESL--CRYLLE 473
           RER   L  +   I G+     +GAFY F+D      S +    ++ NS  +  C Y+LE
Sbjct: 301 RERNQFLTNALNSIAGIHCLLSEGAFYAFVDVRQAI-SRLNTQQILQNSSDIAFCNYVLE 360

Query: 474 KGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAI 511
           K +VA VPG AFG +  +R+S+A S+  LQ AV+RI + +
Sbjct: 361 KAEVAAVPGSAFGCEGYMRLSFATSMDNLQEAVKRIASLL 396

BLAST of MC10g0588 vs. NCBI nr
Match: XP_022136875.1 (bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase [Momordica charantia])

HSP 1 Score: 917 bits (2371), Expect = 0.0
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0

Query: 45  MANSLQNLSNASHLGFRYQFPGADSALEPAFGSVSFPSQLRAPFLKSQQYTKRVQPVMAG 104
           MANSLQNLSNASHLGFRYQFPGADSALEPAFGSVSFPSQLRAPFLKSQQYTKRVQPVMAG
Sbjct: 1   MANSLQNLSNASHLGFRYQFPGADSALEPAFGSVSFPSQLRAPFLKSQQYTKRVQPVMAG 60

Query: 105 SVSDQVIVDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA 164
           SVSDQVIVDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA
Sbjct: 61  SVSDQVIVDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA 120

Query: 165 GMNAIRDGYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVC 224
           GMNAIRDGYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVC
Sbjct: 121 GMNAIRDGYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVC 180

Query: 225 SPGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCS 284
           SPGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCS
Sbjct: 181 SPGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCS 240

Query: 285 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 344
           PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT
Sbjct: 241 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 300

Query: 345 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEA 404
           VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEA
Sbjct: 301 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEA 360

Query: 405 VATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESL 464
           VATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESL
Sbjct: 361 VATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESL 420

Query: 465 CRYLLEKGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV 519
           CRYLLEKGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV
Sbjct: 421 CRYLLEKGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV 475

BLAST of MC10g0588 vs. NCBI nr
Match: XP_038878084.1 (bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 848 bits (2190), Expect = 1.03e-307
Identity = 436/475 (91.79%), Postives = 455/475 (95.79%), Query Frame = 0

Query: 45  MANSLQNLSNASHLGFRYQFPGADSALEPAFGSVSFPSQLRAPFLKSQQYTKRVQPVMAG 104
           MANSLQ L NAS LGFR+QF GADSALE AFGSVSFPSQL AP LKS +YTK+++PVMAG
Sbjct: 1   MANSLQTLPNASRLGFRHQFSGADSALESAFGSVSFPSQLLAPSLKSVKYTKKMKPVMAG 60

Query: 105 SVSDQVIVDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA 164
           ++ D + VD++LSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPI EA
Sbjct: 61  NIPDHMSVDISLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPITEA 120

Query: 165 GMNAIRDGYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVC 224
           G+NAIRDGYTRYTPNAGTLE+RQAICHKLKEENGISYTPDQILVSNGAKQSILQAV+AVC
Sbjct: 121 GINAIRDGYTRYTPNAGTLEVRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVVAVC 180

Query: 225 SPGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCS 284
           SPG EV+IPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLE+KITEKSRLLILCS
Sbjct: 181 SPGDEVIIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLESKITEKSRLLILCS 240

Query: 285 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 344
           PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT
Sbjct: 241 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 300

Query: 345 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEA 404
           VNGFSKAFAMTGWRLGY+AGPKHFVSACGKIQSQSTSGASSISQKA VAALGMGYAGGEA
Sbjct: 301 VNGFSKAFAMTGWRLGYLAGPKHFVSACGKIQSQSTSGASSISQKAAVAALGMGYAGGEA 360

Query: 405 VATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESL 464
           VATMVKAF ERRD LVKSFGE+ GVKISEPQGAFYLFLDFSSYYG+EVEGFGVINNSESL
Sbjct: 361 VATMVKAFGERRDYLVKSFGELAGVKISEPQGAFYLFLDFSSYYGAEVEGFGVINNSESL 420

Query: 465 CRYLLEKGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV 519
           CRYLLEKGQVALVPGDAFGDDSCIRISYAESLS LQAAVERIK A+EA RPVVPV
Sbjct: 421 CRYLLEKGQVALVPGDAFGDDSCIRISYAESLSVLQAAVERIKEALEAARPVVPV 475

BLAST of MC10g0588 vs. NCBI nr
Match: XP_023551093.1 (bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase isoform X1 [Cucurbita pepo subsp. pepo] >KAG6600628.1 hypothetical protein SDJN03_05861, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 844 bits (2181), Expect = 2.41e-306
Identity = 436/475 (91.79%), Postives = 451/475 (94.95%), Query Frame = 0

Query: 45  MANSLQNLSNASHLGFRYQFPGADSALEPAFGSVSFPSQLRAPFLKSQQYTKRVQPVMAG 104
           MANSL  L N S LGFRYQFPGADSA + AFGS+SFPS+L AP LKS QYTK+V+PVMAG
Sbjct: 1   MANSLHTLPNPSRLGFRYQFPGADSASDTAFGSISFPSRLPAPSLKSVQYTKKVKPVMAG 60

Query: 105 SVSDQVIVDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA 164
            VSD V VD++LSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA
Sbjct: 61  HVSDNVSVDISLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA 120

Query: 165 GMNAIRDGYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVC 224
           G+NAIRDGYTRYTPNAGTLE+RQAICHKLKEENGISYTPDQILVSNGAKQSILQAV+AVC
Sbjct: 121 GINAIRDGYTRYTPNAGTLEVRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVVAVC 180

Query: 225 SPGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCS 284
           SPG EV+IPAPFWVSYPEMARLADATPVILPT IE NFLLDPKLLEAKITEKSRLLILCS
Sbjct: 181 SPGDEVIIPAPFWVSYPEMARLADATPVILPTSIEANFLLDPKLLEAKITEKSRLLILCS 240

Query: 285 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 344
           PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT
Sbjct: 241 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 300

Query: 345 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEA 404
           VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKA VAALGMGYAGGEA
Sbjct: 301 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAAVAALGMGYAGGEA 360

Query: 405 VATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESL 464
           VATMVKAFRERRD LVKSFGE+ GVKISEPQGAFYLFLDFSSYYG+E EGFGVINNSESL
Sbjct: 361 VATMVKAFRERRDYLVKSFGELAGVKISEPQGAFYLFLDFSSYYGAEAEGFGVINNSESL 420

Query: 465 CRYLLEKGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV 519
           CRYLLEKGQVALVPGDAFGDD+CIRISYAESLS LQ AVERIK A+EAVRP VPV
Sbjct: 421 CRYLLEKGQVALVPGDAFGDDTCIRISYAESLSILQDAVERIKKALEAVRPAVPV 475

BLAST of MC10g0588 vs. NCBI nr
Match: XP_022942854.1 (bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase isoform X1 [Cucurbita moschata])

HSP 1 Score: 843 bits (2177), Expect = 9.79e-306
Identity = 434/475 (91.37%), Postives = 451/475 (94.95%), Query Frame = 0

Query: 45  MANSLQNLSNASHLGFRYQFPGADSALEPAFGSVSFPSQLRAPFLKSQQYTKRVQPVMAG 104
           MANSL  L N S LGFRYQFPGADSA + AFGS+SFPS+L AP LKS QYTK+V+P+MAG
Sbjct: 1   MANSLHTLPNPSRLGFRYQFPGADSASDTAFGSISFPSRLPAPSLKSVQYTKKVKPIMAG 60

Query: 105 SVSDQVIVDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA 164
            VSD V VD++LSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA
Sbjct: 61  HVSDNVSVDISLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA 120

Query: 165 GMNAIRDGYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVC 224
           G+NAIRDGYTRYTPNAGTLE+RQAICHKLKEENGISYTPDQILVSNGAKQSILQAV+AVC
Sbjct: 121 GINAIRDGYTRYTPNAGTLEVRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVVAVC 180

Query: 225 SPGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCS 284
           SPG EV+IPAPFWVSYPEMARLADATPVILPT IE NFLLDP+LLEAKITEKSRLLILCS
Sbjct: 181 SPGDEVIIPAPFWVSYPEMARLADATPVILPTSIEANFLLDPRLLEAKITEKSRLLILCS 240

Query: 285 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 344
           PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT
Sbjct: 241 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 300

Query: 345 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEA 404
           VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKA VAALGMGYAGGEA
Sbjct: 301 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAAVAALGMGYAGGEA 360

Query: 405 VATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESL 464
           VATMVKAFRERRD LVKSFGE+ GVKISEPQGAFYLFLDFSSYYG+E EGFGVINNSESL
Sbjct: 361 VATMVKAFRERRDYLVKSFGELAGVKISEPQGAFYLFLDFSSYYGAEAEGFGVINNSESL 420

Query: 465 CRYLLEKGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV 519
           CRYLLEKGQVALVPGDAFGDD+CIRISYAESLS LQ AVERIK A+EAVRP VPV
Sbjct: 421 CRYLLEKGQVALVPGDAFGDDTCIRISYAESLSILQDAVERIKKALEAVRPAVPV 475

BLAST of MC10g0588 vs. NCBI nr
Match: XP_022980735.1 (bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase isoform X1 [Cucurbita maxima])

HSP 1 Score: 839 bits (2167), Expect = 3.26e-304
Identity = 434/475 (91.37%), Postives = 449/475 (94.53%), Query Frame = 0

Query: 45  MANSLQNLSNASHLGFRYQFPGADSALEPAFGSVSFPSQLRAPFLKSQQYTKRVQPVMAG 104
           MANSL  L N S LGFRYQFPGADSA + AFGS+SFPS+L AP LKS QYTK+V+PVMAG
Sbjct: 1   MANSLHTLPNPSRLGFRYQFPGADSASDTAFGSISFPSRLPAPSLKSVQYTKKVKPVMAG 60

Query: 105 SVSDQVIVDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA 164
            VS  V VD++LSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA
Sbjct: 61  HVSGNVSVDISLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA 120

Query: 165 GMNAIRDGYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVC 224
           G+NAIRDGYTRYTPNAGTLE+RQAICHKLKEENGISYTPDQILVSNGAKQSILQAV+AVC
Sbjct: 121 GINAIRDGYTRYTPNAGTLEVRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVVAVC 180

Query: 225 SPGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCS 284
           SPG EV+IPAPFWVSYPEMARLADATPVILPT IE NFLLDPKLLEAKITEKSRLLILCS
Sbjct: 181 SPGDEVIIPAPFWVSYPEMARLADATPVILPTSIEANFLLDPKLLEAKITEKSRLLILCS 240

Query: 285 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 344
           PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT
Sbjct: 241 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 300

Query: 345 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEA 404
           VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKA VAALGMGYAGGEA
Sbjct: 301 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAAVAALGMGYAGGEA 360

Query: 405 VATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESL 464
           VATMVKAFRERRD LVKSFGE+ GVKISEPQGAFYLFLDFSSYYG+E  GFGVINNSESL
Sbjct: 361 VATMVKAFRERRDYLVKSFGELAGVKISEPQGAFYLFLDFSSYYGAEAVGFGVINNSESL 420

Query: 465 CRYLLEKGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV 519
           CRYLLEKGQVALVPGDAFGDD+CIRISYAESLS LQ AVERIK A+EAVRP VPV
Sbjct: 421 CRYLLEKGQVALVPGDAFGDDTCIRISYAESLSILQDAVERIKKALEAVRPAVPV 475

BLAST of MC10g0588 vs. ExPASy TrEMBL
Match: A0A6J1C558 (bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase OS=Momordica charantia OX=3673 GN=LOC111008458 PE=3 SV=1)

HSP 1 Score: 917 bits (2371), Expect = 0.0
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0

Query: 45  MANSLQNLSNASHLGFRYQFPGADSALEPAFGSVSFPSQLRAPFLKSQQYTKRVQPVMAG 104
           MANSLQNLSNASHLGFRYQFPGADSALEPAFGSVSFPSQLRAPFLKSQQYTKRVQPVMAG
Sbjct: 1   MANSLQNLSNASHLGFRYQFPGADSALEPAFGSVSFPSQLRAPFLKSQQYTKRVQPVMAG 60

Query: 105 SVSDQVIVDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA 164
           SVSDQVIVDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA
Sbjct: 61  SVSDQVIVDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA 120

Query: 165 GMNAIRDGYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVC 224
           GMNAIRDGYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVC
Sbjct: 121 GMNAIRDGYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVC 180

Query: 225 SPGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCS 284
           SPGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCS
Sbjct: 181 SPGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCS 240

Query: 285 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 344
           PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT
Sbjct: 241 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 300

Query: 345 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEA 404
           VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEA
Sbjct: 301 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEA 360

Query: 405 VATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESL 464
           VATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESL
Sbjct: 361 VATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESL 420

Query: 465 CRYLLEKGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV 519
           CRYLLEKGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV
Sbjct: 421 CRYLLEKGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV 475

BLAST of MC10g0588 vs. ExPASy TrEMBL
Match: A0A6J1FSJ1 (bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447760 PE=3 SV=1)

HSP 1 Score: 843 bits (2177), Expect = 4.74e-306
Identity = 434/475 (91.37%), Postives = 451/475 (94.95%), Query Frame = 0

Query: 45  MANSLQNLSNASHLGFRYQFPGADSALEPAFGSVSFPSQLRAPFLKSQQYTKRVQPVMAG 104
           MANSL  L N S LGFRYQFPGADSA + AFGS+SFPS+L AP LKS QYTK+V+P+MAG
Sbjct: 1   MANSLHTLPNPSRLGFRYQFPGADSASDTAFGSISFPSRLPAPSLKSVQYTKKVKPIMAG 60

Query: 105 SVSDQVIVDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA 164
            VSD V VD++LSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA
Sbjct: 61  HVSDNVSVDISLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA 120

Query: 165 GMNAIRDGYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVC 224
           G+NAIRDGYTRYTPNAGTLE+RQAICHKLKEENGISYTPDQILVSNGAKQSILQAV+AVC
Sbjct: 121 GINAIRDGYTRYTPNAGTLEVRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVVAVC 180

Query: 225 SPGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCS 284
           SPG EV+IPAPFWVSYPEMARLADATPVILPT IE NFLLDP+LLEAKITEKSRLLILCS
Sbjct: 181 SPGDEVIIPAPFWVSYPEMARLADATPVILPTSIEANFLLDPRLLEAKITEKSRLLILCS 240

Query: 285 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 344
           PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT
Sbjct: 241 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 300

Query: 345 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEA 404
           VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKA VAALGMGYAGGEA
Sbjct: 301 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAAVAALGMGYAGGEA 360

Query: 405 VATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESL 464
           VATMVKAFRERRD LVKSFGE+ GVKISEPQGAFYLFLDFSSYYG+E EGFGVINNSESL
Sbjct: 361 VATMVKAFRERRDYLVKSFGELAGVKISEPQGAFYLFLDFSSYYGAEAEGFGVINNSESL 420

Query: 465 CRYLLEKGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV 519
           CRYLLEKGQVALVPGDAFGDD+CIRISYAESLS LQ AVERIK A+EAVRP VPV
Sbjct: 421 CRYLLEKGQVALVPGDAFGDDTCIRISYAESLSILQDAVERIKKALEAVRPAVPV 475

BLAST of MC10g0588 vs. ExPASy TrEMBL
Match: A0A6J1IXC7 (bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480013 PE=3 SV=1)

HSP 1 Score: 839 bits (2167), Expect = 1.58e-304
Identity = 434/475 (91.37%), Postives = 449/475 (94.53%), Query Frame = 0

Query: 45  MANSLQNLSNASHLGFRYQFPGADSALEPAFGSVSFPSQLRAPFLKSQQYTKRVQPVMAG 104
           MANSL  L N S LGFRYQFPGADSA + AFGS+SFPS+L AP LKS QYTK+V+PVMAG
Sbjct: 1   MANSLHTLPNPSRLGFRYQFPGADSASDTAFGSISFPSRLPAPSLKSVQYTKKVKPVMAG 60

Query: 105 SVSDQVIVDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA 164
            VS  V VD++LSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA
Sbjct: 61  HVSGNVSVDISLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA 120

Query: 165 GMNAIRDGYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVC 224
           G+NAIRDGYTRYTPNAGTLE+RQAICHKLKEENGISYTPDQILVSNGAKQSILQAV+AVC
Sbjct: 121 GINAIRDGYTRYTPNAGTLEVRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVVAVC 180

Query: 225 SPGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCS 284
           SPG EV+IPAPFWVSYPEMARLADATPVILPT IE NFLLDPKLLEAKITEKSRLLILCS
Sbjct: 181 SPGDEVIIPAPFWVSYPEMARLADATPVILPTSIEANFLLDPKLLEAKITEKSRLLILCS 240

Query: 285 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 344
           PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT
Sbjct: 241 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 300

Query: 345 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEA 404
           VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKA VAALGMGYAGGEA
Sbjct: 301 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAAVAALGMGYAGGEA 360

Query: 405 VATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESL 464
           VATMVKAFRERRD LVKSFGE+ GVKISEPQGAFYLFLDFSSYYG+E  GFGVINNSESL
Sbjct: 361 VATMVKAFRERRDYLVKSFGELAGVKISEPQGAFYLFLDFSSYYGAEAVGFGVINNSESL 420

Query: 465 CRYLLEKGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV 519
           CRYLLEKGQVALVPGDAFGDD+CIRISYAESLS LQ AVERIK A+EAVRP VPV
Sbjct: 421 CRYLLEKGQVALVPGDAFGDDTCIRISYAESLSILQDAVERIKKALEAVRPAVPV 475

BLAST of MC10g0588 vs. ExPASy TrEMBL
Match: A0A0A0KT58 (Aminotran_1_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G000870 PE=3 SV=1)

HSP 1 Score: 827 bits (2137), Expect = 5.85e-300
Identity = 426/475 (89.68%), Postives = 450/475 (94.74%), Query Frame = 0

Query: 45  MANSLQNLSNASHLGFRYQFPGADSALEPAFGSVSFPSQLRAPFLKSQQYTKRVQPVMAG 104
           MA+SL  L +AS LGFR+QF GADS L+ AFGS+SFPSQ  AP LKS Q TK+V+PVMAG
Sbjct: 1   MASSLHILPDASRLGFRHQFSGADSTLDSAFGSLSFPSQRLAPSLKSVQLTKKVKPVMAG 60

Query: 105 SVSDQVIVDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEA 164
           ++ D V VD++LSPRVNSVKPSKTVAI+DQATALVQAGVPVIRLAAGEPDFDTPAPI EA
Sbjct: 61  NIPDHVSVDISLSPRVNSVKPSKTVAISDQATALVQAGVPVIRLAAGEPDFDTPAPITEA 120

Query: 165 GMNAIRDGYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVC 224
           G+NAIRDGYTRYT NAGTLELRQAICHKLK+ENG+SYTPDQILVSNGAKQSILQAV+AVC
Sbjct: 121 GINAIRDGYTRYTANAGTLELRQAICHKLKDENGLSYTPDQILVSNGAKQSILQAVVAVC 180

Query: 225 SPGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCS 284
           SPG EV+IPAPFWVSYPEMARLADATPVILPT I++NFLLDPKLLE+KITEKSRLLILCS
Sbjct: 181 SPGDEVIIPAPFWVSYPEMARLADATPVILPTHIDNNFLLDPKLLESKITEKSRLLILCS 240

Query: 285 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 344
           PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT
Sbjct: 241 PSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLT 300

Query: 345 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEA 404
           VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKA VAALGMGYAGGEA
Sbjct: 301 VNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAAVAALGMGYAGGEA 360

Query: 405 VATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESL 464
           VATMVKAFRERRD LVKSFGE+ GVKISEPQGAFYLFLDFSSYYG+EVEGFGVINNSESL
Sbjct: 361 VATMVKAFRERRDYLVKSFGELAGVKISEPQGAFYLFLDFSSYYGAEVEGFGVINNSESL 420

Query: 465 CRYLLEKGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV 519
           CRYLLEKGQVALVPGDAFGDD+CIRISYAESLS LQAAVERIK A+EA RPVVPV
Sbjct: 421 CRYLLEKGQVALVPGDAFGDDTCIRISYAESLSVLQAAVERIKKALEAARPVVPV 475

BLAST of MC10g0588 vs. ExPASy TrEMBL
Match: A0A5D3E1J9 (Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00680 PE=3 SV=1)

HSP 1 Score: 827 bits (2137), Expect = 6.31e-300
Identity = 428/477 (89.73%), Postives = 451/477 (94.55%), Query Frame = 0

Query: 45  MANSLQNLSNASHLGFRYQFPGADSA--LEPAFGSVSFPSQLRAPFLKSQQYTKRVQPVM 104
           MANSL  L NAS LGFR+QF GADSA  L+ AFGS+SFPSQL AP LKS Q TK+V+PVM
Sbjct: 1   MANSLHTLPNASRLGFRHQFSGADSASALDSAFGSLSFPSQLLAPSLKSVQLTKKVKPVM 60

Query: 105 AGSVSDQVIVDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIV 164
           AG++ D V VD++LSPRVNSVKPSKTVAI+DQATALVQAGVPVIRLAAGEPDFDTPAPI 
Sbjct: 61  AGNIPDHVSVDISLSPRVNSVKPSKTVAISDQATALVQAGVPVIRLAAGEPDFDTPAPIT 120

Query: 165 EAGMNAIRDGYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIA 224
           EAG+NAIRDGYTRYT NAGTLE+RQAIC KLK+ENG+SYTPDQILVSNGAKQSILQAV+A
Sbjct: 121 EAGINAIRDGYTRYTANAGTLEVRQAICRKLKDENGLSYTPDQILVSNGAKQSILQAVVA 180

Query: 225 VCSPGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLIL 284
           VCSPG EV+IPAPFWVSYPEMARLADATPVILPT I++NFLLDPKLLE+KITEKSRLLIL
Sbjct: 181 VCSPGDEVIIPAPFWVSYPEMARLADATPVILPTHIDNNFLLDPKLLESKITEKSRLLIL 240

Query: 285 CSPSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERT 344
           CSPSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERT
Sbjct: 241 CSPSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERT 300

Query: 345 LTVNGFSKAFAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGG 404
           LTVNGFSKAFAMTGWRLGY+AGPKHFVSACGKIQSQSTSGASSISQKA VAALGMGYAGG
Sbjct: 301 LTVNGFSKAFAMTGWRLGYLAGPKHFVSACGKIQSQSTSGASSISQKAAVAALGMGYAGG 360

Query: 405 EAVATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSE 464
           EAVATMVKAFRERRD LVKSFGE+ GVKISEPQGAFYLFLDFSSYYG+EVEGFGVINNSE
Sbjct: 361 EAVATMVKAFRERRDYLVKSFGELAGVKISEPQGAFYLFLDFSSYYGAEVEGFGVINNSE 420

Query: 465 SLCRYLLEKGQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV 519
           SLCRYLLEKGQVALVPGDAFGDDSCIRISYAESLS LQAAVERIK A+EA RPVVPV
Sbjct: 421 SLCRYLLEKGQVALVPGDAFGDDSCIRISYAESLSVLQAAVERIKEALEAARPVVPV 477

BLAST of MC10g0588 vs. TAIR 10
Match: AT2G22250.2 (aspartate aminotransferase )

HSP 1 Score: 649.0 bits (1673), Expect = 3.1e-186
Identity = 334/450 (74.22%), Postives = 381/450 (84.67%), Query Frame = 0

Query: 76  GSVSFPSQLRAPF--LKSQQYTKRV----QPVMAGSVSDQVIVDLTLSPRVNSVKPSKTV 135
           GSV F   LR  F   KS   + R+    +P  A ++S    VD++LSPRV S+KPSKT+
Sbjct: 28  GSVRFQQPLRLSFSYCKSGNMSSRICAMAKPNDAETLSSS--VDMSLSPRVQSLKPSKTM 87

Query: 136 AITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEAGMNAIRDGYTRYTPNAGTLELRQAI 195
            ITD A  LVQ+GVPVIRLAAGEPDFDTP  + EAG+NAIR+G+TRYT NAG  ELR+AI
Sbjct: 88  VITDLAATLVQSGVPVIRLAAGEPDFDTPKVVAEAGINAIREGFTRYTLNAGITELREAI 147

Query: 196 CHKLKEENGISYTPDQILVSNGAKQSILQAVIAVCSPGHEVVIPAPFWVSYPEMARLADA 255
           C KLKEENG+SY PDQILVSNGAKQS+LQAV+AVCSPG EV+IPAP+WVSY E ARLADA
Sbjct: 148 CRKLKEENGLSYAPDQILVSNGAKQSLLQAVLAVCSPGDEVIIPAPYWVSYTEQARLADA 207

Query: 256 TPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCSPSNPTGSVYPKELLEKIAEIVAKHP 315
           TPV++PT+I +NFLLDPK LE+K+TEKSRLLILCSPSNPTGSVYPK LLE+IA I+AKHP
Sbjct: 208 TPVVIPTKISNNFLLDPKDLESKLTEKSRLLILCSPSNPTGSVYPKSLLEEIARIIAKHP 267

Query: 316 RLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLTVNGFSKAFAMTGWRLGYIAGPKHFV 375
           RLLVLSDEIYEHIIYAPATHTSFASLP M+ERTLTVNGFSKAFAMTGWRLGY+AGPKH V
Sbjct: 268 RLLVLSDEIYEHIIYAPATHTSFASLPDMYERTLTVNGFSKAFAMTGWRLGYLAGPKHIV 327

Query: 376 SACGKIQSQSTSGASSISQKAGVAALGMGYAGGEAVATMVKAFRERRDSLVKSFGEIPGV 435
           +AC K+Q Q +SGASSI+QKAGVAALG+G AGGE VA MVKA+RERRD LVKS G+I GV
Sbjct: 328 AACSKLQGQVSSGASSIAQKAGVAALGLGKAGGETVAEMVKAYRERRDFLVKSLGDIKGV 387

Query: 436 KISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESLCRYLLEKGQVALVPGDAFGDDSCIR 495
           KISEPQGAFYLF+DFS+YYGSE EGFG+IN+S SL  Y L+K QVA+VPGDAFGDDSCIR
Sbjct: 388 KISEPQGAFYLFIDFSAYYGSEAEGFGLINDSSSLALYFLDKFQVAMVPGDAFGDDSCIR 447

Query: 496 ISYAESLSTLQAAVERIKNAIEAVRPVVPV 520
           ISYA SL  LQAAVE+I+ A+E +R  V V
Sbjct: 448 ISYATSLDVLQAAVEKIRKALEPLRATVSV 475

BLAST of MC10g0588 vs. TAIR 10
Match: AT2G22250.3 (aspartate aminotransferase )

HSP 1 Score: 649.0 bits (1673), Expect = 3.1e-186
Identity = 334/450 (74.22%), Postives = 381/450 (84.67%), Query Frame = 0

Query: 76  GSVSFPSQLRAPF--LKSQQYTKRV----QPVMAGSVSDQVIVDLTLSPRVNSVKPSKTV 135
           GSV F   LR  F   KS   + R+    +P  A ++S    VD++LSPRV S+KPSKT+
Sbjct: 28  GSVRFQQPLRLSFSYCKSGNMSSRICAMAKPNDAETLSSS--VDMSLSPRVQSLKPSKTM 87

Query: 136 AITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEAGMNAIRDGYTRYTPNAGTLELRQAI 195
            ITD A  LVQ+GVPVIRLAAGEPDFDTP  + EAG+NAIR+G+TRYT NAG  ELR+AI
Sbjct: 88  VITDLAATLVQSGVPVIRLAAGEPDFDTPKVVAEAGINAIREGFTRYTLNAGITELREAI 147

Query: 196 CHKLKEENGISYTPDQILVSNGAKQSILQAVIAVCSPGHEVVIPAPFWVSYPEMARLADA 255
           C KLKEENG+SY PDQILVSNGAKQS+LQAV+AVCSPG EV+IPAP+WVSY E ARLADA
Sbjct: 148 CRKLKEENGLSYAPDQILVSNGAKQSLLQAVLAVCSPGDEVIIPAPYWVSYTEQARLADA 207

Query: 256 TPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCSPSNPTGSVYPKELLEKIAEIVAKHP 315
           TPV++PT+I +NFLLDPK LE+K+TEKSRLLILCSPSNPTGSVYPK LLE+IA I+AKHP
Sbjct: 208 TPVVIPTKISNNFLLDPKDLESKLTEKSRLLILCSPSNPTGSVYPKSLLEEIARIIAKHP 267

Query: 316 RLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLTVNGFSKAFAMTGWRLGYIAGPKHFV 375
           RLLVLSDEIYEHIIYAPATHTSFASLP M+ERTLTVNGFSKAFAMTGWRLGY+AGPKH V
Sbjct: 268 RLLVLSDEIYEHIIYAPATHTSFASLPDMYERTLTVNGFSKAFAMTGWRLGYLAGPKHIV 327

Query: 376 SACGKIQSQSTSGASSISQKAGVAALGMGYAGGEAVATMVKAFRERRDSLVKSFGEIPGV 435
           +AC K+Q Q +SGASSI+QKAGVAALG+G AGGE VA MVKA+RERRD LVKS G+I GV
Sbjct: 328 AACSKLQGQVSSGASSIAQKAGVAALGLGKAGGETVAEMVKAYRERRDFLVKSLGDIKGV 387

Query: 436 KISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESLCRYLLEKGQVALVPGDAFGDDSCIR 495
           KISEPQGAFYLF+DFS+YYGSE EGFG+IN+S SL  Y L+K QVA+VPGDAFGDDSCIR
Sbjct: 388 KISEPQGAFYLFIDFSAYYGSEAEGFGLINDSSSLALYFLDKFQVAMVPGDAFGDDSCIR 447

Query: 496 ISYAESLSTLQAAVERIKNAIEAVRPVVPV 520
           ISYA SL  LQAAVE+I+ A+E +R  V V
Sbjct: 448 ISYATSLDVLQAAVEKIRKALEPLRATVSV 475

BLAST of MC10g0588 vs. TAIR 10
Match: AT2G22250.1 (aspartate aminotransferase )

HSP 1 Score: 644.8 bits (1662), Expect = 5.9e-185
Identity = 321/408 (78.68%), Postives = 363/408 (88.97%), Query Frame = 0

Query: 112 VDLTLSPRVNSVKPSKTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEAGMNAIRD 171
           VD++LSPRV S+KPSKT+ ITD A  LVQ+GVPVIRLAAGEPDFDTP  + EAG+NAIR+
Sbjct: 21  VDMSLSPRVQSLKPSKTMVITDLAATLVQSGVPVIRLAAGEPDFDTPKVVAEAGINAIRE 80

Query: 172 GYTRYTPNAGTLELRQAICHKLKEENGISYTPDQILVSNGAKQSILQAVIAVCSPGHEVV 231
           G+TRYT NAG  ELR+AIC KLKEENG+SY PDQILVSNGAKQS+LQAV+AVCSPG EV+
Sbjct: 81  GFTRYTLNAGITELREAICRKLKEENGLSYAPDQILVSNGAKQSLLQAVLAVCSPGDEVI 140

Query: 232 IPAPFWVSYPEMARLADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCSPSNPTGS 291
           IPAP+WVSY E ARLADATPV++PT+I +NFLLDPK LE+K+TEKSRLLILCSPSNPTGS
Sbjct: 141 IPAPYWVSYTEQARLADATPVVIPTKISNNFLLDPKDLESKLTEKSRLLILCSPSNPTGS 200

Query: 292 VYPKELLEKIAEIVAKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLTVNGFSKA 351
           VYPK LLE+IA I+AKHPRLLVLSDEIYEHIIYAPATHTSFASLP M+ERTLTVNGFSKA
Sbjct: 201 VYPKSLLEEIARIIAKHPRLLVLSDEIYEHIIYAPATHTSFASLPDMYERTLTVNGFSKA 260

Query: 352 FAMTGWRLGYIAGPKHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEAVATMVKA 411
           FAMTGWRLGY+AGPKH V+AC K+Q Q +SGASSI+QKAGVAALG+G AGGE VA MVKA
Sbjct: 261 FAMTGWRLGYLAGPKHIVAACSKLQGQVSSGASSIAQKAGVAALGLGKAGGETVAEMVKA 320

Query: 412 FRERRDSLVKSFGEIPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESLCRYLLEK 471
           +RERRD LVKS G+I GVKISEPQGAFYLF+DFS+YYGSE EGFG+IN+S SL  Y L+K
Sbjct: 321 YRERRDFLVKSLGDIKGVKISEPQGAFYLFIDFSAYYGSEAEGFGLINDSSSLALYFLDK 380

Query: 472 GQVALVPGDAFGDDSCIRISYAESLSTLQAAVERIKNAIEAVRPVVPV 520
            QVA+VPGDAFGDDSCIRISYA SL  LQAAVE+I+ A+E +R  V V
Sbjct: 381 FQVAMVPGDAFGDDSCIRISYATSLDVLQAAVEKIRKALEPLRATVSV 428

BLAST of MC10g0588 vs. TAIR 10
Match: AT1G77670.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 187.2 bits (474), Expect = 3.4e-47
Identity = 120/391 (30.69%), Postives = 207/391 (52.94%), Query Frame = 0

Query: 127 KTVAITDQATALVQAGVPVIRLAAGEPDFDTPAPIVEAGMNAIRDGYTRYTPNAGTLELR 186
           KT   T  +   V+ G   I L  G P+FD P  + EA + AI+DG  +Y    G  +L 
Sbjct: 65  KTTIFTQMSILAVKHG--AINLGQGFPNFDGPDFVKEAAIQAIKDGKNQYARGYGIPQLN 124

Query: 187 QAICHKLKEENGISYTPD-QILVSNGAKQSILQAVIAVCSPGHEVVIPAPFWVSYPEMAR 246
            AI  + +E+ G+   P+ ++ V++G  ++I  A++ + +PG EV++ APF+ SY     
Sbjct: 125 SAIAARFREDTGLVVDPEKEVTVTSGCTEAIAAAMLGLINPGDEVILFAPFYDSYEATLS 184

Query: 247 LADATPVILPTRIEDNFLLDPKLLEAKITEKSRLLILCSPSNPTGSVYPKELLEKIAEIV 306
           +A A    +  R  D F +  + L+A +T K+R +++ +P NPTG ++ +E LE IA + 
Sbjct: 185 MAGAKVKGITLRPPD-FSIPLEELKAAVTNKTRAILMNTPHNPTGKMFTREELETIASLC 244

Query: 307 AKHPRLLVLSDEIYEHIIYAPATHTSFASLPGMWERTLTVNGFSKAFAMTGWRLGYIAGP 366
            ++  +LV SDE+Y+ + +    H S ASLPGM+ERT+T+N   K F++TGW++G+   P
Sbjct: 245 IEND-VLVFSDEVYDKLAF-EMDHISIASLPGMYERTVTMNSLGKTFSLTGWKIGWAIAP 304

Query: 367 KHFVSACGKIQSQSTSGASSISQKAGVAALGMGYAGGEAVATMVKAFRERRDSLVKSFGE 426
            H      +  S  T   S+ +Q A VAAL    A       + + +  ++++LVK   E
Sbjct: 305 PHLTWGVRQAHSYLTFATSTPAQWAAVAAL---KAPESYFKELKRDYNVKKETLVKGLKE 364

Query: 427 IPGVKISEPQGAFYLFLDFSSYYGSEVEGFGVINNSESLCRYLLEKGQVALVPGDAF--- 486
           + G  +    G +++  D +         FG + N  + C YL+E+  V  +P   F   
Sbjct: 365 V-GFTVFPSSGTYFVVADHTP--------FG-MENDVAFCEYLIEEVGVVAIPTSVFYLN 424

Query: 487 --GDDSCIRISYAESLSTLQAAVERIKNAIE 512
                + +R ++ +   TL+ A+ER+K  ++
Sbjct: 425 PEEGKNLVRFAFCKDEETLRGAIERMKQKLK 437

BLAST of MC10g0588 vs. TAIR 10
Match: AT1G80360.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 128.6 bits (322), Expect = 1.4e-29
Identity = 102/372 (27.42%), Postives = 173/372 (46.51%), Query Frame = 0

Query: 148 LAAGEPDFDTPAPIVEAGMNAIRDG-YTRYTPNAGTLELRQAICHKLKEENGISYTPDQI 207
           LA G   +  P   +E     + D   + Y P+ G  ELRQA+  KL+EEN +  T  Q+
Sbjct: 35  LAQGVVHWQPPQKALEKVKELVWDPIISSYGPDEGLPELRQALLKKLREENKL--TNSQV 94

Query: 208 LVSNGAKQSILQAVIAVCSPGHEVVIPAPFWVSYPEMARLADATPVILPTRIEDNFLLDP 267
           +V+ GA Q+ +  VI +C  G  VV+  P++ +     ++   T +I+     D    D 
Sbjct: 95  MVTAGANQAFVNLVITLCDAGDSVVMFEPYYFNSYMAFQMTGVTNIIVGPGQSDTLYPDA 154

Query: 268 KLLEAKITEKS---RLLILCSPSNPTGSVYPKELLEKIAEIVAKHPRLLVLSDEIYEHII 327
             LE  ++E     +++ + +P NP+G+  P+ LL++IA+I  K     ++ D  YE+ +
Sbjct: 155 DWLERTLSESKPTPKVVTVVNPGNPSGTYVPEPLLKRIAQI-CKDAGCWLIVDNTYEYFM 214

Query: 328 YAPATHTSFASLPGMWERTLTVNGFSKAFAMTGWRLGYIAGPKH---FVSACGKIQSQST 387
           Y    H          +  + V  FSK + M GWRLGYIA  +    F +   KIQ    
Sbjct: 215 YDGLKHCCVEG-----DHIVNVFSFSKTYGMMGWRLGYIAYSERLDGFATELVKIQDNIP 274

Query: 388 SGASSISQKAGVAALGMGYAGGEAVATMVKAFRERRDSLVKSFGEIPGVKISEPQGAFYL 447
             A+ ISQ+  V AL     G   +   VK+  + RD + ++   +    +   +GA YL
Sbjct: 275 ICAAIISQRLAVYAL---EEGSGWITERVKSLVKNRDIVKEALEPLGKENVKGGEGAIYL 334

Query: 448 FLDFSSYYGSEVEGFGVINNSESLCRYLLEKGQVALVPGDAFGDDSCIRISYAESLST-L 507
           +      +  + +          + R+L  +  V ++PG A G    +R+S+       +
Sbjct: 335 WAKLPEGHRDDFK----------VVRWLAHRHGVVVIPGCASGSPGYLRVSFGGLQEVEM 385

Query: 508 QAAVERIKNAIE 512
           +AA  R++  IE
Sbjct: 395 RAAAARLRKGIE 385

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
E9L7A52.6e-20979.54Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate amino... [more]
Q9SIE14.4e-18574.22Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate amino... [more]
Q5F4K88.0e-17976.79Aspartate aminotransferase OS=Pinus pinaster OX=71647 GN=AAT PE=1 SV=1[more]
Q8KDS83.7e-9145.57Aspartate/prephenate aminotransferase OS=Chlorobaculum tepidum (strain ATCC 4965... [more]
Q82WA83.8e-8843.25Aspartate/prephenate aminotransferase OS=Nitrosomonas europaea (strain ATCC 1971... [more]
Match NameE-valueIdentityDescription
XP_022136875.10.0100.00bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate amino... [more]
XP_038878084.11.03e-30791.79bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate amino... [more]
XP_023551093.12.41e-30691.79bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate amino... [more]
XP_022942854.19.79e-30691.37bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate amino... [more]
XP_022980735.13.26e-30491.37bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate amino... [more]
Match NameE-valueIdentityDescription
A0A6J1C5580.0100.00bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate amino... [more]
A0A6J1FSJ14.74e-30691.37bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate amino... [more]
A0A6J1IXC71.58e-30491.37bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate amino... [more]
A0A0A0KT585.85e-30089.68Aminotran_1_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G0008... [more]
A0A5D3E1J96.31e-30089.73Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate amino... [more]
Match NameE-valueIdentityDescription
AT2G22250.23.1e-18674.22aspartate aminotransferase [more]
AT2G22250.33.1e-18674.22aspartate aminotransferase [more]
AT2G22250.15.9e-18578.68aspartate aminotransferase [more]
AT1G77670.13.4e-4730.69Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT1G80360.11.4e-2927.42Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 129..504
e-value: 4.7E-151
score: 504.7
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 175..397
e-value: 4.7E-151
score: 504.7
IPR004839Aminotransferase, class I/classIIPFAMPF00155Aminotran_1_2coord: 144..506
e-value: 2.7E-72
score: 244.0
NoneNo IPR availablePANTHERPTHR43795:SF64GLUTAMATE-OXALOACETATE TRANSAMINASE5coord: 74..515
NoneNo IPR availablePANTHERPTHR43795BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATEDcoord: 74..515
NoneNo IPR availableCDDcd00609AAT_likecoord: 146..507
e-value: 2.57012E-114
score: 340.088
IPR004838Aminotransferases, class-I, pyridoxal-phosphate-binding sitePROSITEPS00105AA_TRANSFER_CLASS_1coord: 347..360
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 116..511

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0588.1MC10g0588.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009058 biosynthetic process
biological_process GO:0006520 cellular amino acid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0008483 transaminase activity
molecular_function GO:0003824 catalytic activity