MC10g0538 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g0538
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	tRNA N6-adenosine threonylcarbamoyltransferase
Location	MC10: 4476549 .. 4479912 (+)
RNA-Seq Expression	MC10g0538
Synteny	MC10g0538

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

AACTTTTCAAAATATGCTTTTCTGCCCGAAAAAACTGTTACAAAACTGTACTTGGGTGTATTTCAATCATCTCTCGCGTTCTCTCTCCCATGCTCTCTTGCCTTTTCAAATCCAAACTCAAAAAAATCTGTGCTCGTTTTTTCAGGAGTTCGATCGATTTTCTTTGGACCACTTGAAATACCATTTTACTCAAGTTTTTACTGTTTGCGTCGATTTTAAGGTTAGGATTTTTTATTTTCCGGCTATTTGAGGGGTCGGAGTTTTGACATCGGTGGTTTCTGTTTGATTTTTGGTGGTTTTAGGGTGTGATTTCTGGTTTGAGGTGGTGGGAGGTTGAAGACGAAGGACGTACTGGTCAGTTCTACTTTCTCTCTTCTACTACTTCATACCCAGTTTCTAGTTAGACAGATTAAATGATGCATATTGCAGTAGTTTTGCGTTTTGCGTTTTGATTATCATACAAGCTTCTAATTTCATCTGCCACTTTTCAGTTTTTGTTTCTTTGACTTCGTCTCAATTTTGGCCTTCTTATAGTTAACTTCTGTTCTGTTTGTTGCTATCCGACTCCGGAATTCACGGTTTTTCAGGATTGAATTTTTCAGTTTTTTGTTTTCTACAGGGTTTTTCGAATCTTTTGAAAAATGAAGAAAATGATAGCTCTTGGTTTTGAGGGTTCAGCTAACAAGATTGGTGTTGGGGTTGTGACCTTAGATGGGGATATTTTATCAAACCCACGCCACACTTACATTACACCTCCTGGGCATGGCTTCCTTCCTCGCGAAACTGCACATCACCATCTTCAGCACATTCTTCCATTGGTTAAGTCTGCTCTGGAAACTGCCAAAATTACGCCAGAGGATATTGACTGCCTCTGTTACACTAAAGGGCCTGGCATGGGAGCTCCACTACAGGTTTCTGCTGTTGCTGTGAGGGTTCTTTCTCAGATTTGGAATAAGCCTGTTGTTGCTGTTAACCACTGTGTTGCTCATATTGAAATGGGGAGGATTGTGACTGGTGCTGATGATCCTGTTGTCTTGTATGTTAGTGGTGGGAATACTCAGGTGATTGCTTACAGTGAAGGAAGGTATAGAATCTTTGGGGAAACTATTGATATTGCGGTCGGAAACTGCTTGGACAGGTTTGCAAGGGTCCTAACATTATCTAATGATCCAAGTCCTGGATACAATATTGAACAGGTGTGTTGTTGAAAAGTGATTATATAGGTTTTTCAACTATTCCATGTATTCTTTTCATTGGATTATAGTAAGGGATGTAGAACCTTCATAGTGCAAGCGAGTCTGAAAAGGAATCTTTGATAGAAGTTTTAGCTTTTATGTTCTTGGATTGGAAGAAACTAATGAATAAGTCTTATTCAAGGATTTTCACCGAAACGTGTGAAACGTTGTAAATTATGTATGATCTTTGCTTGCTTACCATTTTAGAAGGTGCATGTGTTACTTCAGTTTTTTTAATATGCATATGATATGTGATATTTGAAGAAGAAATTTACAGTGATATGGCATAATATTCAAGGTGGGAAAGATGTTATAAGCAAAAATGCTCCTTGTTTCCATTTGATAGTTTAGAAAAGGAAAAAGGAAATAGCAACTTGTGCTTGTGTGACTTTATTGCTATTTTTATTTAGGTACAATTACAGTAATACGAACCTTTCACAGTGGTCAACTGTAATCTTCATCCTAGGGGGATATTTGAACATTCTTTTCTTATTGAAGTTGATAAATGTAGTAGAAGGGTTCGTTTATTTAACAAGATGTTTTTTACACCCTTAAATGTATCATAGCATAGTTTCATTGGTTCAAGATCTGAAATACACCAGTGTTTCAGTTTTCCCTACTTGCTATTTTATGTTTCAAATGGCTGGATGATTTTAACAATTTGTATGGTGGTTTAAATGTGGTGGTTGTAAATTGATCCTTGTTCTTTATTTTCGTGATATAATTAATTTGAAATGTGTTTTAGCTGAAAACATTTTAAATACATTTTGTGTTTTTCTGCTGGAAAAATGTGTCTGAGTCTGCCTTCGTGCAGTTGCCCTGTTTTAACTCTTGATTTATTGCAATTACATATACCTTTGCACTTTTCCTTTTACATCACTTTTTTTTTGTTGCTGAATCCCAGAAAGTGCTTGAGCTATATAATGTGTTAGAAAGTGCTTTCTTTGACTTGATCTATCGATTTTCTTCATCCACAGCTTGCAAAGAAGGGAGAAAAGTTTATCGAACTTCCATATGTTGTGAAAGGGATGGACGTATCATTCAGTGGGATATTAAGCTATATTGAAGCCACTGCTGAGGAGAAGCTAAAATGTAATGAGTGTACTCCTGCTGACTTGTGCTACTCTCTACAGGTAATTTGTATTTACTATGATATTGGGGTTTGTCTCCATTTCTTTGATGGTCTATAAACCTTGGGCCATTCTATAATTAAACTGAGTTTGTGAGTTGGGTTCTGCTCTTTCATGGATGATCTCAAACTTCGAGCAAATTTGTAAAACTATTGTACACTCTGCCACTGCCATTCTTATGTCAAATAACTTACAAATGCTATTCCATTGACTTTCAATGTACTGCTCTGTGTTCTAGGAAACATTATTTGCTATGCTTGTGGAAATCACAGAACGAGCCATGGCTCACTGCGACAAGAAAGATGTTCTTATAGTGGGTGGCGTAGGTTGTAACGAGCGCTTGCAAGAGATGATGAGAATAATGTGCTCTGAGCGAGGTGGGAGGTTATTTGCAACTGATGATCGTTATTGTATCGATAACGGGGCAATGATCGCGTATACTGGTCTTCTTTCTTGGGCACATGGCATATCAACACCATTGGAGGAAGTAACATTCACCCAGCGGTTTCGAACCGATGAAGTACATGCAATCTGGAGAGAAGAAAAAAGATTTGACTGATGGAAATGAACTCATGAAAGGAGACGTCTAAACCATAGGGTGCGTCTTTTATCTTCCTTAAGAGTTAGAGTTAGAGTTGATATGGCAATTAGGGGTTTTGTAAGCCTAGTGTAACTGAAATAATTGTAATTGGATTTTCCATGGCTCTTTAGGTGTCGCCTGAATTTTTATAGTTTAGCATCACTGAAAACTTGGGCAGATGTTCTTTAGGATTCAACTTGTAAATGGTATTTATTTACAGGTTGGACATAAAATAGAACACAGGAAATGAGAGAAACTCTGAAGGTAAAGATCTGTGCTATGGTTGATGAAAGGCTGAAGAACTTCAGCCTTTGCACCTTATTTTTGGGCATCTTGTGTACTTTTATTGTAATTTAGAAACATTGTTCTTAAAACAAATATTGCTCGTGACTATTAGAGTGTGTTTGAGAGTGATGGCA

mRNA sequence

AACTTTTCAAAATATGCTTTTCTGCCCGAAAAAACTGTTACAAAACTGTACTTGGGTGTATTTCAATCATCTCTCGCGTTCTCTCTCCCATGCTCTCTTGCCTTTTCAAATCCAAACTCAAAAAAATCTGTGCTCGTTTTTTCAGGAGTTCGATCGATTTTCTTTGGACCACTTGAAATACCATTTTACTCAAGTTTTTACTGTTTGCGTCGATTTTAAGGGTGTGATTTCTGGTTTGAGGTGGTGGGAGGTTGAAGACGAAGGACGTACTGGGTTTTTCGAATCTTTTGAAAAATGAAGAAAATGATAGCTCTTGGTTTTGAGGGTTCAGCTAACAAGATTGGTGTTGGGGTTGTGACCTTAGATGGGGATATTTTATCAAACCCACGCCACACTTACATTACACCTCCTGGGCATGGCTTCCTTCCTCGCGAAACTGCACATCACCATCTTCAGCACATTCTTCCATTGGTTAAGTCTGCTCTGGAAACTGCCAAAATTACGCCAGAGGATATTGACTGCCTCTGTTACACTAAAGGGCCTGGCATGGGAGCTCCACTACAGGTTTCTGCTGTTGCTGTGAGGGTTCTTTCTCAGATTTGGAATAAGCCTGTTGTTGCTGTTAACCACTGTGTTGCTCATATTGAAATGGGGAGGATTGTGACTGGTGCTGATGATCCTGTTGTCTTGTATGTTAGTGGTGGGAATACTCAGGTGATTGCTTACAGTGAAGGAAGGTATAGAATCTTTGGGGAAACTATTGATATTGCGGTCGGAAACTGCTTGGACAGGTTTGCAAGGGTCCTAACATTATCTAATGATCCAAGTCCTGGATACAATATTGAACAGCTTGCAAAGAAGGGAGAAAAGTTTATCGAACTTCCATATGTTGTGAAAGGGATGGACGTATCATTCAGTGGGATATTAAGCTATATTGAAGCCACTGCTGAGGAGAAGCTAAAATGTAATGAGTGTACTCCTGCTGACTTGTGCTACTCTCTACAGGAAACATTATTTGCTATGCTTGTGGAAATCACAGAACGAGCCATGGCTCACTGCGACAAGAAAGATGTTCTTATAGTGGGTGGCGTAGGTTGTAACGAGCGCTTGCAAGAGATGATGAGAATAATGTGCTCTGAGCGAGGTGGGAGGTTATTTGCAACTGATGATCGTTATTGTATCGATAACGGGGCAATGATCGCGTATACTGGTCTTCTTTCTTGGGCACATGGCATATCAACACCATTGGAGGAAGTAACATTCACCCAGCGGTTTCGAACCGATGAAGTACATGCAATCTGGAGAGAAGAAAAAAGATTTGACTGATGGAAATGAACTCATGAAAGGAGACGTCTAAACCATAGGGTGCGTCTTTTATCTTCCTTAAGAGTTAGAGTTAGAGTTGATATGGCAATTAGGGGTTTTGTAAGCCTAGTGTAACTGAAATAATTGTAATTGGATTTTCCATGGCTCTTTAGGTGTCGCCTGAATTTTTATAGTTTAGCATCACTGAAAACTTGGGCAGATGTTCTTTAGGATTCAACTTGTAAATGGTATTTATTTACAGGTTGGACATAAAATAGAACACAGGAAATGAGAGAAACTCTGAAGGTAAAGATCTGTGCTATGGTTGATGAAAGGCTGAAGAACTTCAGCCTTTGCACCTTATTTTTGGGCATCTTGTGTACTTTTATTGTAATTTAGAAACATTGTTCTTAAAACAAATATTGCTCGTGACTATTAGAGTGTGTTTGAGAGTGATGGCA

Coding sequence (CDS)

ATGAAGAAAATGATAGCTCTTGGTTTTGAGGGTTCAGCTAACAAGATTGGTGTTGGGGTTGTGACCTTAGATGGGGATATTTTATCAAACCCACGCCACACTTACATTACACCTCCTGGGCATGGCTTCCTTCCTCGCGAAACTGCACATCACCATCTTCAGCACATTCTTCCATTGGTTAAGTCTGCTCTGGAAACTGCCAAAATTACGCCAGAGGATATTGACTGCCTCTGTTACACTAAAGGGCCTGGCATGGGAGCTCCACTACAGGTTTCTGCTGTTGCTGTGAGGGTTCTTTCTCAGATTTGGAATAAGCCTGTTGTTGCTGTTAACCACTGTGTTGCTCATATTGAAATGGGGAGGATTGTGACTGGTGCTGATGATCCTGTTGTCTTGTATGTTAGTGGTGGGAATACTCAGGTGATTGCTTACAGTGAAGGAAGGTATAGAATCTTTGGGGAAACTATTGATATTGCGGTCGGAAACTGCTTGGACAGGTTTGCAAGGGTCCTAACATTATCTAATGATCCAAGTCCTGGATACAATATTGAACAGCTTGCAAAGAAGGGAGAAAAGTTTATCGAACTTCCATATGTTGTGAAAGGGATGGACGTATCATTCAGTGGGATATTAAGCTATATTGAAGCCACTGCTGAGGAGAAGCTAAAATGTAATGAGTGTACTCCTGCTGACTTGTGCTACTCTCTACAGGAAACATTATTTGCTATGCTTGTGGAAATCACAGAACGAGCCATGGCTCACTGCGACAAGAAAGATGTTCTTATAGTGGGTGGCGTAGGTTGTAACGAGCGCTTGCAAGAGATGATGAGAATAATGTGCTCTGAGCGAGGTGGGAGGTTATTTGCAACTGATGATCGTTATTGTATCGATAACGGGGCAATGATCGCGTATACTGGTCTTCTTTCTTGGGCACATGGCATATCAACACCATTGGAGGAAGTAACATTCACCCAGCGGTTTCGAACCGATGAAGTACATGCAATCTGGAGAGAAGAAAAAAGATTTGACTGA

Protein sequence

MKKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLVKSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMGRIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWREEKRFD

Homology

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BLAST of MC10g0538 vs. ExPASy Swiss-Prot
Match: A7SXZ6 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Nematostella vectensis OX=45351 GN=osgep PE=3 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 3.0e-142
Identity = 233/332 (70.18%), Postives = 276/332 (83.13%), Query Frame = 0


Query: 7   LGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLVKSALET 66
           +GFEGSANK+G+G++  DG +LSNPRHTYITPPG GF+PR+TA HH +H + +++ AL+ 
Sbjct: 5   IGFEGSANKLGIGIIR-DGVVLSNPRHTYITPPGQGFMPRDTAKHHQEHAIDILRRALDE 64

Query: 67  AKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMGRIVTGA 126
           A+I P+DIDC+CYTKGPGMGAPL   AV  R ++Q+W KP++ VNHC+ HIEMGR++TGA
Sbjct: 65  AQIRPQDIDCICYTKGPGMGAPLVAVAVVARTVAQLWKKPIIGVNHCIGHIEMGRLITGA 124

Query: 127 DDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQL 186
           ++P VLYVSGGNTQVIAY + RYRIFGETIDIAVGNCLDRFARVL LSNDPSPGYNIEQ+
Sbjct: 125 NNPTVLYVSGGNTQVIAYLQKRYRIFGETIDIAVGNCLDRFARVLKLSNDPSPGYNIEQM 184

Query: 187 AKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETLFAMLVE 246
           AKKG+K IELPY VKGMDVSFSGILSYIE  A + L   ECTP DLC+SLQET+FAMLVE
Sbjct: 185 AKKGKKLIELPYTVKGMDVSFSGILSYIECMAHKLLSSEECTPEDLCFSLQETVFAMLVE 244

Query: 247 ITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTG 306
            TERAMAHC   +VLIVGGVGCN+RLQEMM +M  ERG +L+ATD+R+CIDNGAMIA  G
Sbjct: 245 TTERAMAHCGSNEVLIVGGVGCNKRLQEMMDVMAKERGAKLYATDERFCIDNGAMIAQAG 304

Query: 307 LLSWAHGISTPLEEVTFTQRFRTDEVHAIWRE 339
              +  G  TPLE+ T TQRFRTDEV   WR+
Sbjct: 305 WEMFQTGSVTPLEQTTCTQRFRTDEVEVTWRD 335

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BLAST of MC10g0538 vs. ExPASy Swiss-Prot
Match: Q0VCI1 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Bos taurus OX=9913 GN=OSGEP PE=2 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 2.0e-138
Identity = 236/332 (71.08%), Postives = 269/332 (81.02%), Query Frame = 0


Query: 7   LGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLVKSALET 66
           LGFEGSANKIGVGVV  DG +L+NPR TY+TPPG GFLP +TA HH   IL L++ AL  
Sbjct: 5   LGFEGSANKIGVGVVR-DGKVLANPRRTYVTPPGTGFLPGDTARHHRAVILDLLQEALTE 64

Query: 67  AKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMGRIVTGA 126
           A +T EDIDC+ YTKGPGMGAPL   AV  R ++Q+WNKP++ VNHC+ HIEMGR++TGA
Sbjct: 65  AGLTSEDIDCIAYTKGPGMGAPLVSVAVVARTVAQLWNKPLLGVNHCIGHIEMGRLITGA 124

Query: 127 DDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQL 186
            +P VLYVSGGNTQVIAYSE RYRIFGETIDIAVGNCLDRFARVL +SNDPSPGYNIEQ+
Sbjct: 125 TNPTVLYVSGGNTQVIAYSEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQM 184

Query: 187 AKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETLFAMLVE 246
           AK+G+K +ELPY VKGMDVSFSGILS+IE  A+  L   ECTP DLC+SLQET+FAMLVE
Sbjct: 185 AKRGKKLVELPYTVKGMDVSFSGILSFIEDVAQRMLATGECTPEDLCFSLQETVFAMLVE 244

Query: 247 ITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTG 306
           ITERAMAHC  ++ LIVGGVGCN RLQEMM  MC ERG RLFATD+R+CIDNGAMIA  G
Sbjct: 245 ITERAMAHCGSQEALIVGGVGCNVRLQEMMETMCQERGARLFATDERFCIDNGAMIAQAG 304

Query: 307 LLSWAHGISTPLEEVTFTQRFRTDEVHAIWRE 339
              +  G  TPL E   TQR+RTDEV   WR+
Sbjct: 305 WEMFQAGHRTPLSESGITQRYRTDEVEVTWRD 335

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BLAST of MC10g0538 vs. ExPASy Swiss-Prot
Match: Q7SYR1 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Xenopus laevis OX=8355 GN=osgep PE=2 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 5.9e-138
Identity = 230/334 (68.86%), Postives = 273/334 (81.74%), Query Frame = 0


Query: 5   IALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLVKSAL 64
           I +GFEGSANKIGVG++  DG +LSNPR TYITPPG GF+P +TA HH   IL +++ AL
Sbjct: 3   IVVGFEGSANKIGVGIIQ-DGKVLSNPRRTYITPPGQGFMPSDTARHHRSCILDVLQEAL 62

Query: 65  ETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMGRIVT 124
           E + I PED+DC+ YTKGPGMGAPL   A+  R ++Q+W KP++ VNHC+ HIEMGR++T
Sbjct: 63  EESNIKPEDVDCVAYTKGPGMGAPLLSVAIVARTVAQLWKKPLLGVNHCIGHIEMGRLIT 122

Query: 125 GADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIE 184
           GA++P VLYVSGGNTQVIAYSE  YRIFGETIDIAVGNCLDRFARVL +SNDPSPGYNIE
Sbjct: 123 GAENPTVLYVSGGNTQVIAYSERCYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIE 182

Query: 185 QLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETLFAML 244
           Q+AKKG+KF+ELPY VKGMDVSFSGILSYIE  + + L   ECTP DLC+SLQETLF+ML
Sbjct: 183 QMAKKGKKFVELPYTVKGMDVSFSGILSYIEDMSHKMLSSGECTPEDLCFSLQETLFSML 242

Query: 245 VEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAY 304
           VEITERAMAHC  ++VLIVGGVGCN RLQEMM +MC ERG ++FATD+R+CIDNGAMIA 
Sbjct: 243 VEITERAMAHCGSQEVLIVGGVGCNVRLQEMMGVMCEERGAKIFATDERFCIDNGAMIAQ 302

Query: 305 TGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWRE 339
            G   +  G  T L++   TQR+RTDEV   WR+
Sbjct: 303 AGWEMFRAGQVTNLQDSWITQRYRTDEVEVTWRD 335

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BLAST of MC10g0538 vs. ExPASy Swiss-Prot
Match: Q9WVS2 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Rattus norvegicus OX=10116 GN=Osgep PE=2 SV=2)

HSP 1 Score: 489.6 bits (1259), Expect = 2.9e-137
Identity = 233/332 (70.18%), Postives = 270/332 (81.33%), Query Frame = 0


Query: 7   LGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLVKSALET 66
           LGFEGSANKIGVGVV  DG +L+NPR TY+T PG GFLP +TA HH   IL L++ AL  
Sbjct: 5   LGFEGSANKIGVGVVR-DGTVLANPRRTYVTAPGTGFLPGDTARHHRAVILDLLQEALTE 64

Query: 67  AKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMGRIVTGA 126
           A +TP+DIDC+ YTKGPGMGAPL   AV  R ++Q+WNKP++ VNHC+ HIEMGR++TGA
Sbjct: 65  AGLTPKDIDCIAYTKGPGMGAPLASVAVVARTVAQLWNKPLLGVNHCIGHIEMGRLITGA 124

Query: 127 DDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQL 186
            +P VLYVSGGNTQVI+YSE RYRIFGETIDIAVGNCLDRFARVL +SNDPSPGYNIEQ+
Sbjct: 125 VNPTVLYVSGGNTQVISYSEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQM 184

Query: 187 AKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETLFAMLVE 246
           AK+G+K +ELPY VKGMDVSFSGILS+IE  A+  L   ECTP DLC+SLQET+FAMLVE
Sbjct: 185 AKRGKKLVELPYTVKGMDVSFSGILSFIEDAAQRMLATGECTPEDLCFSLQETVFAMLVE 244

Query: 247 ITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTG 306
           ITERAMAHC  K+ LIVGGVGCN RLQEMM  MC ERG +LFATD+R+CIDNGAMIA  G
Sbjct: 245 ITERAMAHCGSKEALIVGGVGCNVRLQEMMATMCQERGAQLFATDERFCIDNGAMIAQAG 304

Query: 307 LLSWAHGISTPLEEVTFTQRFRTDEVHAIWRE 339
              +  G  TPL++   TQR+RTDEV   WR+
Sbjct: 305 WEMFQAGHRTPLQDSGITQRYRTDEVEVTWRD 335

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BLAST of MC10g0538 vs. ExPASy Swiss-Prot
Match: Q9NPF4 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Homo sapiens OX=9606 GN=OSGEP PE=1 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 3.8e-137
Identity = 234/332 (70.48%), Postives = 267/332 (80.42%), Query Frame = 0


Query: 7   LGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLVKSALET 66
           LGFEGSANKIGVGVV  DG +L+NPR TY+TPPG GFLP +TA HH   IL L++ AL  
Sbjct: 5   LGFEGSANKIGVGVVR-DGKVLANPRRTYVTPPGTGFLPGDTARHHRAVILDLLQEALTE 64

Query: 67  AKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMGRIVTGA 126
           + +T +DIDC+ YTKGPGMGAPL   AV  R ++Q+WNKP+V VNHC+ HIEMGR++TGA
Sbjct: 65  SGLTSQDIDCIAYTKGPGMGAPLVSVAVVARTVAQLWNKPLVGVNHCIGHIEMGRLITGA 124

Query: 127 DDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQL 186
             P VLYVSGGNTQVIAYSE RYRIFGETIDIAVGNCLDRFARVL +SNDPSPGYNIEQ+
Sbjct: 125 TSPTVLYVSGGNTQVIAYSEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQM 184

Query: 187 AKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETLFAMLVE 246
           AK+G+K +ELPY VKGMDVSFSGILS+IE  A   L   ECTP DLC+SLQET+FAMLVE
Sbjct: 185 AKRGKKLVELPYTVKGMDVSFSGILSFIEDVAHRMLATGECTPEDLCFSLQETVFAMLVE 244

Query: 247 ITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTG 306
           ITERAMAHC  ++ LIVGGVGCN RLQEMM  MC ERG RLFATD+R+CIDNGAMIA  G
Sbjct: 245 ITERAMAHCGSQEALIVGGVGCNVRLQEMMATMCQERGARLFATDERFCIDNGAMIAQAG 304

Query: 307 LLSWAHGISTPLEEVTFTQRFRTDEVHAIWRE 339
              +  G  TPL +   TQR+RTDEV   WR+
Sbjct: 305 WEMFRAGHRTPLSDSGVTQRYRTDEVEVTWRD 335

[Close]

BLAST of MC10g0538 vs. NCBI nr
Match: XP_022136896.1 (probable tRNA N6-adenosine threonylcarbamoyltransferase [Momordica charantia])

HSP 1 Score: 700 bits (1807), Expect = 1.42e-254
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLV 60
           MKKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLV
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLV 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMG 120
           KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMG
Sbjct: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETL 240
           YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETL
Sbjct: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETL 240

Query: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300
           FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA
Sbjct: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300

Query: 301 MIAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWREEKRFD 343
           MIAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWREEKRFD
Sbjct: 301 MIAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWREEKRFD 343

[Close]

BLAST of MC10g0538 vs. NCBI nr
Match: XP_023525719.1 (probable tRNA N6-adenosine threonylcarbamoyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 681 bits (1756), Expect = 1.10e-246
Identity = 331/340 (97.35%), Postives = 338/340 (99.41%), Query Frame = 0


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLV 60
           MKKMIALGFEGSANKIGVGVVTLDG+ILSNPRHTYITPPGHGFLPRETAHHH +HILPL+
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLI 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMG 120
           KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKP+VAVNHCVAHIEMG
Sbjct: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETL 240
           YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLK NECTPADLCYSLQETL
Sbjct: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKSNECTPADLCYSLQETL 240

Query: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300
           FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA
Sbjct: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300

Query: 301 MIAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWREEK 340
           MIAYTGLL+WAHG+STPLEEVTFTQRFRTDEVHAIWRE+K
Sbjct: 301 MIAYTGLLAWAHGMSTPLEEVTFTQRFRTDEVHAIWREKK 340

[Close]

BLAST of MC10g0538 vs. NCBI nr
Match: XP_022941751.1 (probable tRNA N6-adenosine threonylcarbamoyltransferase [Cucurbita moschata] >XP_022999783.1 probable tRNA N6-adenosine threonylcarbamoyltransferase [Cucurbita maxima] >KAG7031304.1 putative tRNA N6-adenosine threonylcarbamoyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 681 bits (1756), Expect = 1.10e-246
Identity = 331/340 (97.35%), Postives = 338/340 (99.41%), Query Frame = 0


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLV 60
           MKKMIALGFEGSANKIGVGVVTLDG+ILSNPRHTYITPPGHGFLPRETAHHH +HILPL+
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLI 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMG 120
           KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKP+VAVNHCVAHIEMG
Sbjct: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETL 240
           YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLK NECTPADLCYSLQETL
Sbjct: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKSNECTPADLCYSLQETL 240

Query: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300
           FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA
Sbjct: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300

Query: 301 MIAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWREEK 340
           MIAYTGLL+WAHG+STPLEEVTFTQRFRTDEVHAIWRE+K
Sbjct: 301 MIAYTGLLAWAHGMSTPLEEVTFTQRFRTDEVHAIWREKK 340

[Close]

BLAST of MC10g0538 vs. NCBI nr
Match: KAG6600665.1 (putative tRNA N6-adenosine threonylcarbamoyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 680 bits (1754), Expect = 2.23e-246
Identity = 330/340 (97.06%), Postives = 338/340 (99.41%), Query Frame = 0


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLV 60
           MKKMIALGFEGSANKIGVGVVTLDG+ILSNPRHTYITPPGHGFLPRETAHHH +HILPL+
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLI 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMG 120
           KSA+ETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKP+VAVNHCVAHIEMG
Sbjct: 61  KSAMETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETL 240
           YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLK NECTPADLCYSLQETL
Sbjct: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKSNECTPADLCYSLQETL 240

Query: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300
           FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA
Sbjct: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300

Query: 301 MIAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWREEK 340
           MIAYTGLL+WAHG+STPLEEVTFTQRFRTDEVHAIWRE+K
Sbjct: 301 MIAYTGLLAWAHGMSTPLEEVTFTQRFRTDEVHAIWREKK 340

[Close]

BLAST of MC10g0538 vs. NCBI nr
Match: XP_038905629.1 (probable tRNA N6-adenosine threonylcarbamoyltransferase [Benincasa hispida] >XP_038905630.1 probable tRNA N6-adenosine threonylcarbamoyltransferase [Benincasa hispida] >XP_038905631.1 probable tRNA N6-adenosine threonylcarbamoyltransferase [Benincasa hispida])

HSP 1 Score: 671 bits (1731), Expect = 5.29e-243
Identity = 326/338 (96.45%), Postives = 333/338 (98.52%), Query Frame = 0


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLV 60
           MK MIALGFEGSANKIGVGVVTLDG+ILSNPRHTYITPPGHGFLPRETAHHHL HILPLV
Sbjct: 1   MKNMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHLHHILPLV 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMG 120
           KSALETAKITP+DIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKP+VAVNHCVAHIEMG
Sbjct: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           R+VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETL 240
           YNIEQLAKKG+ FIELPYVVKGMDVSFSGILSYIEATAEEKLK NECTPADLCYSLQETL
Sbjct: 181 YNIEQLAKKGKLFIELPYVVKGMDVSFSGILSYIEATAEEKLKSNECTPADLCYSLQETL 240

Query: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300
           FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA
Sbjct: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300

Query: 301 MIAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWRE 338
           MIAYTGLL+WAHG+STPLEE TFTQRFRTDEVHAIWRE
Sbjct: 301 MIAYTGLLAWAHGVSTPLEEATFTQRFRTDEVHAIWRE 338

[Close]

BLAST of MC10g0538 vs. ExPASy TrEMBL
Match: A0A6J1C4S6 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Momordica charantia OX=3673 GN=LOC111008475 PE=3 SV=1)

HSP 1 Score: 700 bits (1807), Expect = 6.90e-255
Identity = 343/343 (100.00%), Postives = 343/343 (100.00%), Query Frame = 0


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLV 60
           MKKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLV
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLV 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMG 120
           KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMG
Sbjct: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETL 240
           YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETL
Sbjct: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETL 240

Query: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300
           FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA
Sbjct: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300

Query: 301 MIAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWREEKRFD 343
           MIAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWREEKRFD
Sbjct: 301 MIAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWREEKRFD 343

[Close]

BLAST of MC10g0538 vs. ExPASy TrEMBL
Match: A0A6J1KKP2 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111494026 PE=3 SV=1)

HSP 1 Score: 681 bits (1756), Expect = 5.34e-247
Identity = 331/340 (97.35%), Postives = 338/340 (99.41%), Query Frame = 0


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLV 60
           MKKMIALGFEGSANKIGVGVVTLDG+ILSNPRHTYITPPGHGFLPRETAHHH +HILPL+
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLI 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMG 120
           KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKP+VAVNHCVAHIEMG
Sbjct: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETL 240
           YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLK NECTPADLCYSLQETL
Sbjct: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKSNECTPADLCYSLQETL 240

Query: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300
           FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA
Sbjct: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300

Query: 301 MIAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWREEK 340
           MIAYTGLL+WAHG+STPLEEVTFTQRFRTDEVHAIWRE+K
Sbjct: 301 MIAYTGLLAWAHGMSTPLEEVTFTQRFRTDEVHAIWREKK 340

[Close]

BLAST of MC10g0538 vs. ExPASy TrEMBL
Match: A0A6J1FLZ0 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447025 PE=3 SV=1)

HSP 1 Score: 681 bits (1756), Expect = 5.34e-247
Identity = 331/340 (97.35%), Postives = 338/340 (99.41%), Query Frame = 0


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLV 60
           MKKMIALGFEGSANKIGVGVVTLDG+ILSNPRHTYITPPGHGFLPRETAHHH +HILPL+
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLI 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMG 120
           KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKP+VAVNHCVAHIEMG
Sbjct: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETL 240
           YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLK NECTPADLCYSLQETL
Sbjct: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKSNECTPADLCYSLQETL 240

Query: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300
           FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA
Sbjct: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300

Query: 301 MIAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWREEK 340
           MIAYTGLL+WAHG+STPLEEVTFTQRFRTDEVHAIWRE+K
Sbjct: 301 MIAYTGLLAWAHGMSTPLEEVTFTQRFRTDEVHAIWREKK 340

[Close]

BLAST of MC10g0538 vs. ExPASy TrEMBL
Match: A0A5A7T3P8 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=GCP2 PE=3 SV=1)

HSP 1 Score: 669 bits (1726), Expect = 2.15e-242
Identity = 325/338 (96.15%), Postives = 334/338 (98.82%), Query Frame = 0


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLV 60
           MKKMIALGFEGSANKIGVGVVTLDG+IL+NPRHTYITPPGHGFLPRETA HHL HILPLV
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGNILANPRHTYITPPGHGFLPRETAQHHLHHILPLV 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMG 120
           KSALETAKITP+DIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKP+VAVNHCVAHIEMG
Sbjct: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           R+VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETL 240
           YNIEQLAKKG+ FIELPYVVKGMDVSFSGILSYIE+TAEEKLK NECTPADLCYSLQETL
Sbjct: 181 YNIEQLAKKGKLFIELPYVVKGMDVSFSGILSYIESTAEEKLKSNECTPADLCYSLQETL 240

Query: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300
           FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA
Sbjct: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300

Query: 301 MIAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWRE 338
           MIAYTGLL+WAHG+STPLEEVTFTQRFRTDEVHAIWRE
Sbjct: 301 MIAYTGLLAWAHGVSTPLEEVTFTQRFRTDEVHAIWRE 338

[Close]

BLAST of MC10g0538 vs. ExPASy TrEMBL
Match: A0A5D3DJA8 (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=GCP2 PE=3 SV=1)

HSP 1 Score: 669 bits (1726), Expect = 2.15e-242
Identity = 325/338 (96.15%), Postives = 334/338 (98.82%), Query Frame = 0


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLV 60
           MKKMIALGFEGSANKIGVGVVTLDG+ILSNPRHTYITPPGHGFLPRETA HHL HILPLV
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMG 120
           KSALETAKITP+DIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKP+VAVNHCVAHIEMG
Sbjct: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           R+VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETL 240
           YNIEQLAKKG+ FIELPYVVKGMDVSFSGILSYIE+TAEEKLK NECTPADLCYSLQETL
Sbjct: 181 YNIEQLAKKGKLFIELPYVVKGMDVSFSGILSYIESTAEEKLKSNECTPADLCYSLQETL 240

Query: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGA 300
           FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMC+ERGGRLFATDDRYCIDNGA
Sbjct: 241 FAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCTERGGRLFATDDRYCIDNGA 300

Query: 301 MIAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWRE 338
           MIAYTGLL+WAHG+STPLEEVTFTQRFRTDEVHAIWRE
Sbjct: 301 MIAYTGLLAWAHGVSTPLEEVTFTQRFRTDEVHAIWRE 338

[Close]

BLAST of MC10g0538 vs. TAIR 10
Match: AT4G22720.1 (Actin-like ATPase superfamily protein )

HSP 1 Score: 641.0 bits (1652), Expect = 5.6e-184
Identity = 301/339 (88.79%), Postives = 325/339 (95.87%), Query Frame = 0


Query: 2   KKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLVK 61
           KKMIA+GFEGSANKIGVG+VTLDG IL+NPRHTYITPPGHGFLPRETAHHHL H+LPLVK
Sbjct: 3   KKMIAIGFEGSANKIGVGIVTLDGTILANPRHTYITPPGHGFLPRETAHHHLDHVLPLVK 62

Query: 62  SALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMGR 121
           SALET+++TPE+IDC+CYTKGPGMGAPLQVSA+ VRVLSQ+W KP+VAVNHCVAHIEMGR
Sbjct: 63  SALETSQVTPEEIDCICYTKGPGMGAPLQVSAIVVRVLSQLWKKPIVAVNHCVAHIEMGR 122

Query: 122 IVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGY 181
           +VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVL LSNDPSPGY
Sbjct: 123 VVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLKLSNDPSPGY 182

Query: 182 NIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETLF 241
           NIEQLAKKGE FI+LPY VKGMDVSFSGILSYIE TAEEKLK NECTPADLCYSLQET+F
Sbjct: 183 NIEQLAKKGENFIDLPYAVKGMDVSFSGILSYIETTAEEKLKNNECTPADLCYSLQETVF 242

Query: 242 AMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAM 301
           AMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMR MCSER G+LFATDDRYCIDNGAM
Sbjct: 243 AMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRTMCSERDGKLFATDDRYCIDNGAM 302

Query: 302 IAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWREEK 341
           IAYTGLL++ +GI TP+E+ TFTQRFRTDEVHA+WRE++
Sbjct: 303 IAYTGLLAFVNGIETPIEDSTFTQRFRTDEVHAVWREKE 341

[Close]

BLAST of MC10g0538 vs. TAIR 10
Match: AT4G22720.2 (Actin-like ATPase superfamily protein )

HSP 1 Score: 641.0 bits (1652), Expect = 5.6e-184
Identity = 301/339 (88.79%), Postives = 325/339 (95.87%), Query Frame = 0


Query: 2   KKMIALGFEGSANKIGVGVVTLDGDILSNPRHTYITPPGHGFLPRETAHHHLQHILPLVK 61
           KKMIA+GFEGSANKIGVG+VTLDG IL+NPRHTYITPPGHGFLPRETAHHHL H+LPLVK
Sbjct: 3   KKMIAIGFEGSANKIGVGIVTLDGTILANPRHTYITPPGHGFLPRETAHHHLDHVLPLVK 62

Query: 62  SALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMGR 121
           SALET+++TPE+IDC+CYTKGPGMGAPLQVSA+ VRVLSQ+W KP+VAVNHCVAHIEMGR
Sbjct: 63  SALETSQVTPEEIDCICYTKGPGMGAPLQVSAIVVRVLSQLWKKPIVAVNHCVAHIEMGR 122

Query: 122 IVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGY 181
           +VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVL LSNDPSPGY
Sbjct: 123 VVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLKLSNDPSPGY 182

Query: 182 NIEQLAKKGEKFIELPYVVKGMDVSFSGILSYIEATAEEKLKCNECTPADLCYSLQETLF 241
           NIEQLAKKGE FI+LPY VKGMDVSFSGILSYIE TAEEKLK NECTPADLCYSLQET+F
Sbjct: 183 NIEQLAKKGENFIDLPYAVKGMDVSFSGILSYIETTAEEKLKNNECTPADLCYSLQETVF 242

Query: 242 AMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAM 301
           AMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMR MCSER G+LFATDDRYCIDNGAM
Sbjct: 243 AMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRTMCSERDGKLFATDDRYCIDNGAM 302

Query: 302 IAYTGLLSWAHGISTPLEEVTFTQRFRTDEVHAIWREEK 341
           IAYTGLL++ +GI TP+E+ TFTQRFRTDEVHA+WRE++
Sbjct: 303 IAYTGLLAFVNGIETPIEDSTFTQRFRTDEVHAVWREKE 341

[Close]

BLAST of MC10g0538 vs. TAIR 10
Match: AT2G45270.1 (glycoprotease 1 )

HSP 1 Score: 119.4 bits (298), Expect = 5.7e-27
Identity = 93/329 (28.27%), Postives = 153/329 (46.50%), Query Frame = 0


Query: 4   MIALGFEGSANKIGVGVVTLDGDILSN--PRHTYITPPGHGFLPRETAHHHLQHILPLVK 63
           ++ LG E S +     VV  +G+ILS        +     G  P++    H + I  +V+
Sbjct: 84  LVVLGIETSCDDTAAAVVRGNGEILSQVISSQAELLVQYGGVAPKQAEEAHSRVIDKVVQ 143

Query: 64  SALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPVVAVNHCVAHIEMGR 123
            AL+ A +T +D+  +  T GPG+   L+V     R ++  ++ P+V V+H  AH  + R
Sbjct: 144 DALDKANLTEKDLSAVAVTIGPGLSLCLRVGVRKARRVAGNFSLPIVGVHHMEAHALVAR 203

Query: 124 IVTG--ADDPVVLYVSGG-NTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPS 183
           +V    +   + L +SGG N  V+A+  G+Y   G T+D A+G   D+ A+ L L    S
Sbjct: 204 LVEQELSFPFMALLISGGHNLLVLAHKLGQYTQLGTTVDDAIGEAFDKTAKWLGLDMHRS 263

Query: 184 PGYNIEQLAKKGE----KFIELPYVVKGMDVSFSGILSYIEATAEEK---LKC------- 243
            G  +E+LA +G+    KF       K  + S++G+ + +    E K    KC       
Sbjct: 264 GGPAVEELALEGDAKSVKFNVPMKYHKDCNFSYAGLKTQVRLAIEAKEIDAKCPVSSATN 323

Query: 244 -NECTPADLCYSLQETLFAMLVEITERAM-----AHCDKKDVLIVGGVGCNERLQEMMRI 303
            +    AD+  S Q      L E  ERA+          K ++I GGV  N+ ++  +  
Sbjct: 324 EDRRNRADIAASFQRVAVLHLEEKCERAIDWALELEPSIKHMVISGGVASNKYVRLRLNN 383

Query: 304 MCSERGGRLFATDDRYCIDNGAMIAYTGL 308
           +   +  +L       C DNG M+A+TGL
Sbjct: 384 IVENKNLKLVCPPPSLCTDNGVMVAWTGL 412

The following BLAST results are available for this feature:

BLAST of MC10g0538 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7SXZ6	3.0e-142	70.18	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Nematostella vectensi...	[more]
Q0VCI1	2.0e-138	71.08	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Bos taurus OX=9913 GN...	[more]
Q7SYR1	5.9e-138	68.86	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Xenopus laevis OX=835...	[more]
Q9WVS2	2.9e-137	70.18	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Rattus norvegicus OX=...	[more]
Q9NPF4	3.8e-137	70.48	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Homo sapiens OX=9606 ...	[more]

BLAST of MC10g0538 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136896.1	1.42e-254	100.00	probable tRNA N6-adenosine threonylcarbamoyltransferase [Momordica charantia]	[more]
XP_023525719.1	1.10e-246	97.35	probable tRNA N6-adenosine threonylcarbamoyltransferase [Cucurbita pepo subsp. p...	[more]
XP_022941751.1	1.10e-246	97.35	probable tRNA N6-adenosine threonylcarbamoyltransferase [Cucurbita moschata] >XP...	[more]
KAG6600665.1	2.23e-246	97.06	putative tRNA N6-adenosine threonylcarbamoyltransferase, partial [Cucurbita argy...	[more]
XP_038905629.1	5.29e-243	96.45	probable tRNA N6-adenosine threonylcarbamoyltransferase [Benincasa hispida] >XP_...	[more]

BLAST of MC10g0538 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C4S6	6.90e-255	100.00	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Momordica charantia O...	[more]
A0A6J1KKP2	5.34e-247	97.35	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Cucurbita maxima OX=3...	[more]
A0A6J1FLZ0	5.34e-247	97.35	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Cucurbita moschata OX...	[more]
A0A5A7T3P8	2.15e-242	96.15	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Cucumis melo var. mak...	[more]
A0A5D3DJA8	2.15e-242	96.15	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Cucumis melo var. mak...	[more]

BLAST of MC10g0538 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G22720.1	5.6e-184	88.79	Actin-like ATPase superfamily protein	[more]
AT4G22720.2	5.6e-184	88.79	Actin-like ATPase superfamily protein	[more]
AT2G45270.1	5.7e-27	28.27	glycoprotease 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017861	Kae1/TsaD family	PRINTS	PR00789	OSIALOPTASE	coord: 98..117 score: 41.74 coord: 7..20 score: 44.41 coord: 77..97 score: 36.44 coord: 260..269 score: 55.65 coord: 130..142 score: 57.19 coord: 152..173 score: 50.4
IPR017861	Kae1/TsaD family	TIGRFAM	TIGR00329	TIGR00329	coord: 7..303 e-value: 4.0E-82 score: 273.8
None	No IPR available	GENE3D	3.30.420.40		coord: 128..290 e-value: 1.5E-97 score: 328.2
None	No IPR available	GENE3D	3.30.420.40		coord: 7..308 e-value: 1.5E-97 score: 328.2
None	No IPR available	CDD	cd00012	NBD_sugar-kinase_HSP70_actin	coord: 7..175 e-value: 1.82921E-5 score: 42.9612
IPR000905	Gcp-like domain	PFAM	PF00814	TsaD	coord: 30..304 e-value: 4.4E-91 score: 305.3
IPR000905	Gcp-like domain	PANTHER	PTHR11735	TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE	coord: 1..339
IPR034680	tRNA N6-adenosine threonylcarbamoyltransferase Kae1, archaea and eukaryote	PANTHER	PTHR11735:SF14	TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE-RELATED	coord: 1..339
IPR034680	tRNA N6-adenosine threonylcarbamoyltransferase Kae1, archaea and eukaryote	HAMAP	MF_01446	Kae1	coord: 4..337 score: 42.362846
IPR043129	ATPase, nucleotide binding domain	SUPERFAMILY	53067	Actin-like ATPase domain	coord: 4..304

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g0538.1	MC10g0538.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002949	tRNA threonylcarbamoyladenosine modification
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000408	EKC/KEOPS complex
cellular_component	GO:0005634	nucleus
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0061711	N(6)-L-threonylcarbamoyladenine synthase activity