MC10g0533 (gene) Bitter gourd (Dali-11) v1

BLAST of MC10g0533 vs. ExPASy Swiss-Prot
Match: Q9FKQ1 (Protein DETOXIFICATION 27 OS=Arabidopsis thaliana OX=3702 GN=DTX27 PE=2 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 1.2e-195
Identity = 345/475 (72.63%), Postives = 401/475 (84.42%), Query Frame = 0

Query: 10  EANVPLLQPKSQIQEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFAG 69
           E+ V LL+     +E   D E L  R+  E++KLW IVGPAIFSRV +YSMLVITQAFAG
Sbjct: 11  ESRVALLKSPHTAEE---DGEGLKDRILVETKKLWQIVGPAIFSRVTTYSMLVITQAFAG 70

Query: 70  HLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVLF 129
           HLGDLELAA+SI NNV VGF+FGLLLGMASALETLCGQA+GAKK HMLG+YMQRSWIVLF
Sbjct: 71  HLGDLELAAISIVNNVTVGFNFGLLLGMASALETLCGQAFGAKKYHMLGVYMQRSWIVLF 130

Query: 130 ICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLKT 189
            CC+ LLP Y+F TP LK LGQP D+AELSG V++ +IPLHF+F   FPLQRFLQ QLK 
Sbjct: 131 FCCVLLLPTYIFTTPVLKFLGQPDDIAELSGVVAIWVIPLHFAFTLSFPLQRFLQCQLKN 190

Query: 190 AAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLTW 249
              AY + VALVVH+LV WL V GLKLG+VGT  TI+ SWWV V  LL+Y+  GGCPLTW
Sbjct: 191 RVTAYAAAVALVVHILVCWLFVDGLKLGVVGTVATISISWWVNVLILLVYSTCGGCPLTW 250

Query: 250 TGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTING 309
           TG S EA +GLWEF+KLSA+SG+MLCLENWYYRILI+M GNL+NA+IAVD+LS+CM ING
Sbjct: 251 TGLSSEALTGLWEFLKLSASSGVMLCLENWYYRILIIMTGNLQNARIAVDSLSICMAING 310

Query: 310 WELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIFDSKIAL 369
           WE+MIPLAFFAG+GVRVANELGAGNGKGA+FAT+V+V  S+IIGLFFWVLIM+  ++IA 
Sbjct: 311 WEMMIPLAFFAGTGVRVANELGAGNGKGARFATIVSVTQSLIIGLFFWVLIMLLHNQIAW 370

Query: 370 IFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLPL 429
           IF+SS AVL  V KLS+LLAF++LLNSVQP+LSGVAVGSGWQSYVAY+NLGCYY IG+PL
Sbjct: 371 IFSSSVAVLDAVNKLSLLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYCIGVPL 430

Query: 430 GFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKWA 485
           GFLMG GF  GVMGIW GMIFGGTA+QT+ILS +T+RCDW+KEA+KAS  I KW+
Sbjct: 431 GFLMGWGFKLGVMGIWGGMIFGGTAVQTMILSFITMRCDWEKEAQKASARINKWS 482

BLAST of MC10g0533 vs. ExPASy Swiss-Prot
Match: Q1PDX9 (Protein DETOXIFICATION 26 OS=Arabidopsis thaliana OX=3702 GN=DTX26 PE=2 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 3.1e-180
Identity = 315/475 (66.32%), Postives = 392/475 (82.53%), Query Frame = 0

Query: 9   EEANVPLLQPKSQIQEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFA 68
           EEA VPLL+  +  +E       +   +W E++K+W+IVGP+IF+ +A+YS+L+ITQAFA
Sbjct: 12  EEATVPLLECHNAAEE----GGGMKREIWIETKKIWYIVGPSIFTGLATYSILIITQAFA 71

Query: 69  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVL 128
           GHLGDLELAA+SI NN  +GF++GLLLGMASALETLCGQA+GA++ +MLG+YMQR WI+L
Sbjct: 72  GHLGDLELAAISIINNFTLGFNYGLLLGMASALETLCGQAFGAREYYMLGVYMQRYWIIL 131

Query: 129 FICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLK 188
           F+CCI LLP+YLFATP LK +GQ  D+AEL+G +++ +IP+HF+FAF FPL RFLQ QLK
Sbjct: 132 FLCCILLLPMYLFATPILKFIGQSDDIAELTGTIALWVIPVHFAFAFFFPLNRFLQCQLK 191

Query: 189 TAAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLT 248
              IA  + V+L VH+LV W  VYG KLG++GT  ++N  WW+ +F L +Y+  GGC LT
Sbjct: 192 NKVIAISAGVSLAVHILVCWFFVYGYKLGIIGTMASVNVPWWLNIFILFLYSTRGGCTLT 251

Query: 249 WTGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTIN 308
           WTGFS EAF+GL E  KLSA+SG+MLCLENWYY+IL++M GNL NAKIAVD+LS+CM++N
Sbjct: 252 WTGFSSEAFTGLLELTKLSASSGIMLCLENWYYKILMLMTGNLVNAKIAVDSLSICMSVN 311

Query: 309 GWELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIFDSKIA 368
           GWE+MIPLAFFAG+GVRVANELGAGNGKGA+FAT+V++  S++IGLFF V+I+IF  +I 
Sbjct: 312 GWEMMIPLAFFAGTGVRVANELGAGNGKGARFATIVSITLSLMIGLFFTVIIVIFHDQIG 371

Query: 369 LIFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLP 428
            IF+SS+AVL  V  LSVLLAF++LLNSVQP+LSGVAVGSGWQSYVAY+NLGCYYLIGLP
Sbjct: 372 SIFSSSEAVLNAVDNLSVLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLP 431

Query: 429 LGFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKW 484
            G  MG  F  GV GIWAGMIFGGTAIQTLIL I+T RCDWD EA K+S+ IKKW
Sbjct: 432 FGLTMGWIFKFGVKGIWAGMIFGGTAIQTLILIIITTRCDWDNEAHKSSVRIKKW 482

BLAST of MC10g0533 vs. ExPASy Swiss-Prot
Match: Q9FNC1 (Protein DETOXIFICATION 28 OS=Arabidopsis thaliana OX=3702 GN=DTX28 PE=3 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 8.4e-170
Identity = 300/476 (63.03%), Postives = 381/476 (80.04%), Query Frame = 0

Query: 9   EEANVPLLQPKSQIQEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFA 68
           E+A +PLL+ ++  +E   ++  +   +W E++KLW IVGPAIF+RV +  + VITQAFA
Sbjct: 13  EKAKIPLLKDQNVAEE---ENGEIKKEIWLETKKLWRIVGPAIFTRVTTNLIFVITQAFA 72

Query: 69  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVL 128
           GHLG+LELAA+SI NNVI+GF++ L +GMA+ALETLCGQA+GAKK  M G+Y+QRSWIVL
Sbjct: 73  GHLGELELAAISIVNNVIIGFNYSLFIGMATALETLCGQAFGAKKYDMFGVYLQRSWIVL 132

Query: 129 FICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLK 188
           F+  I LLP+Y+FATP LK +GQP D+AELSG +S+  IP HFSFAF FP+ RFLQ QLK
Sbjct: 133 FLFSILLLPMYIFATPILKFMGQPDDIAELSGIISVWAIPTHFSFAFFFPINRFLQCQLK 192

Query: 189 TAAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLT 248
            + IA  S V+LVVH+ V WL VY L+LG++GT  T N SWW+ VF L  YT  GGCPLT
Sbjct: 193 NSVIAISSGVSLVVHIFVCWLFVYVLELGVIGTIATANVSWWLNVFILFTYTTCGGCPLT 252

Query: 249 WTGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTIN 308
           WTGFS+E+F+ LWEF KLSA+SG+M+CLENWYYR+LIVM GNLE+A+I VD++S+CM+IN
Sbjct: 253 WTGFSMESFTRLWEFTKLSASSGIMVCLENWYYRMLIVMTGNLEDARIDVDSMSICMSIN 312

Query: 309 GWELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIFDSKIA 368
           G E+M+PLAFFAG+ VRVANELGAGNGK A+FA +++V  S+IIG+   VLI     +I 
Sbjct: 313 GLEMMVPLAFFAGTSVRVANELGAGNGKRARFAMIISVTQSLIIGIIISVLIYFLLDQIG 372

Query: 369 LIFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLP 428
            +F+SS+ VLK V  LS+LL+F+ILLNSVQP+LSGVAVGSGWQS VA++NLGCYY IGLP
Sbjct: 373 WMFSSSETVLKAVNNLSILLSFAILLNSVQPVLSGVAVGSGWQSLVAFINLGCYYFIGLP 432

Query: 429 LGFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKWA 485
           LG +MG  F  GV GIWAGMIFGGT +QTLIL  +T+RCDW+KEA+ A + + KW+
Sbjct: 433 LGIVMGWMFKFGVKGIWAGMIFGGTMVQTLILIFITMRCDWEKEAQNAKVRVNKWS 485

BLAST of MC10g0533 vs. ExPASy Swiss-Prot
Match: Q8W488 (Protein DETOXIFICATION 21 OS=Arabidopsis thaliana OX=3702 GN=DTX21 PE=1 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 6.3e-125
Identity = 233/474 (49.16%), Postives = 327/474 (68.99%), Query Frame = 0

Query: 10  EANVPLLQPKSQIQEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFAG 69
           E    LL+  ++   +  D+  L  +VW ES+KLW +  PAIF+R +++ + +I+Q+F G
Sbjct: 7   ELTAALLKKTAENGGEEKDELGLKQKVWIESKKLWIVAAPAIFTRFSTFGVSIISQSFIG 66

Query: 70  HLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVLF 129
           HLG +ELAA SI   V++ F  G+LLGMASALETLCGQAYGAK+ HMLGIY+QRSWIVL 
Sbjct: 67  HLGPIELAAYSITFTVLLRFSNGILLGMASALETLCGQAYGAKQNHMLGIYLQRSWIVLT 126

Query: 130 ICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLKT 189
            C I L P+Y+F+ P L  LGQ   +  ++  +++ +I ++FSF   F  Q FLQ+Q K 
Sbjct: 127 GCTICLTPVYIFSGPILLALGQEERIVRVARIIALWVIGINFSFVPSFTCQMFLQAQSKN 186

Query: 190 AAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLTW 249
             IAYV+ V+L VHV +SWLL+     G+ G   +   ++W+     L++   GGC  TW
Sbjct: 187 KIIAYVAAVSLGVHVFLSWLLMVHFNFGITGAMTSTLVAFWLPNIAQLLFVTCGGCKDTW 246

Query: 250 TGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTING 309
            GFS+ AF  LW   KLS +SG MLCLE WY  IL+++ GNL+NA++A+DAL++C+ ING
Sbjct: 247 RGFSMMAFKDLWPVFKLSMSSGGMLCLELWYNSILVLLTGNLKNAEVALDALAICLNING 306

Query: 310 WELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIFDSKIAL 369
            E+MI L F A + VRV+NELG+GN KGAKFAT+ AV TS+ +G+  + + +    +++ 
Sbjct: 307 LEMMIALGFLAAASVRVSNELGSGNPKGAKFATLTAVFTSLSLGIVLFFVFLFLRGRVSY 366

Query: 370 IFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLPL 429
           IFT+S+AV  EV  LS LLAFSIL+NSVQP+LSGVAVG+GWQ YV YVNL CYYL+G+P+
Sbjct: 367 IFTTSEAVAAEVADLSPLLAFSILMNSVQPVLSGVAVGAGWQGYVTYVNLACYYLVGIPI 426

Query: 430 GFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKW 484
           G ++G      V G+W GM+F G  +QT +L++MT+R DWD++   +   + +W
Sbjct: 427 GIILGYVVGLQVKGVWIGMLF-GIFVQTCVLTVMTLRTDWDQQVSTSLRRLNRW 479

BLAST of MC10g0533 vs. ExPASy Swiss-Prot
Match: Q9SX83 (Protein DETOXIFICATION 33 OS=Arabidopsis thaliana OX=3702 GN=DTX33 PE=2 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 1.8e-124
Identity = 233/486 (47.94%), Postives = 332/486 (68.31%), Query Frame = 0

Query: 9   EEANVPLLQPKSQIQEQYHDDEPLSTRVWA-----ESRKLWHIVGPAIFSRVASYSMLVI 68
           ++  +PLL P+   +         +++VWA     ES++LW + GPAIF+ ++ YS+  +
Sbjct: 3   KDKTLPLLDPR---EPPELTGTKSASKVWAKEFGEESKRLWELAGPAIFTAISQYSLGAL 62

Query: 69  TQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQR 128
           TQ F+G LG+LELAA+S+ N+VI G  FG++LGM SALETLCGQAYGA ++ M+GIYMQR
Sbjct: 63  TQTFSGRLGELELAAVSVENSVISGLAFGVMLGMGSALETLCGQAYGAGQIRMMGIYMQR 122

Query: 129 SWIVLFICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFL 188
           SW++LF   +FLLP+Y++A P L   G+   +++ +GK ++ +IP  F++A  FP+Q+FL
Sbjct: 123 SWVILFTTALFLLPVYIWAPPILSFFGEAPHISKAAGKFALWMIPQLFAYAANFPIQKFL 182

Query: 189 QSQLKTAAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSG 248
           QSQ K   +A++S V LV+H + SWL +   K GLVG A+T+N SWW++V G L+Y +  
Sbjct: 183 QSQRKVLVMAWISGVVLVIHAVFSWLFILYFKWGLVGAAITLNTSWWLIVIGQLLYILIT 242

Query: 249 GCPLTWTGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSV 308
                WTGFS+ AF  L+ FVKLS AS LMLCLE WY  +L+V+ G L N  I VDA+S+
Sbjct: 243 KSDGAWTGFSMLAFRDLYGFVKLSLASALMLCLEFWYLMVLVVVTGLLPNPLIPVDAISI 302

Query: 309 CMTINGWELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIF 368
           CM I GW  MI + F A   VRV+NELGAGN   AKF+ +V  +TS +IG+   ++++  
Sbjct: 303 CMNIEGWTAMISIGFNAAISVRVSNELGAGNAALAKFSVIVVSITSTLIGIVCMIVVLAT 362

Query: 369 DSKIALIFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYY 428
                 +FTSS+AV  E  +++VLL F++LLNS+QP+LSGVAVG+GWQ+ VAYVN+ CYY
Sbjct: 363 KDSFPYLFTSSEAVAAETTRIAVLLGFTVLLNSLQPVLSGVAVGAGWQALVAYVNIACYY 422

Query: 429 LIGLPLGFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKW 488
           +IGLP G ++G   + GV GIW GM+  G  +QTLIL  +    +W+KEAE+A   +++W
Sbjct: 423 IIGLPAGLVLGFTLDLGVQGIWGGMV-AGICLQTLILIGIIYFTNWNKEAEQAESRVQRW 482

Query: 489 AEETPE 490
                E
Sbjct: 483 GGTAQE 484

BLAST of MC10g0533 vs. NCBI nr
Match: XP_022941750.1 (protein DETOXIFICATION 27-like [Cucurbita moschata])

HSP 1 Score: 816 bits (2107), Expect = 2.33e-295
Identity = 418/482 (86.72%), Postives = 450/482 (93.36%), Query Frame = 0

Query: 9   EEANVPLLQPKSQIQEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFA 68
           +E NVPLLQ KS IQE+  DD PLSTR+  ESRKLWHIVGPA+FSRVASYSMLVITQAFA
Sbjct: 8   QEPNVPLLQSKSLIQEE--DDAPLSTRIRVESRKLWHIVGPAVFSRVASYSMLVITQAFA 67

Query: 69  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVL 128
           GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKK HMLGIYMQRSWIVL
Sbjct: 68  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVL 127

Query: 129 FICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLK 188
           FICCIFLLPIYLFATPALKLLGQP+DLAEL+GKV++LL+PLHFSFAFQFPLQRFLQSQLK
Sbjct: 128 FICCIFLLPIYLFATPALKLLGQPADLAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLK 187

Query: 189 TAAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLT 248
           TA IAYVSLVAL+VH+LVSW  V GLKLGL GTA+T+N SWWVLVFGLLIYTVSGGCP T
Sbjct: 188 TAVIAYVSLVALLVHILVSWWFVSGLKLGLFGTAITMNISWWVLVFGLLIYTVSGGCPHT 247

Query: 249 WTGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTIN 308
           WTGFS+E FSGLW+FVKLSAASGLMLCLENWYYRILIVM GNL+NAKIAVDALSVCMTIN
Sbjct: 248 WTGFSIETFSGLWDFVKLSAASGLMLCLENWYYRILIVMTGNLDNAKIAVDALSVCMTIN 307

Query: 309 GWELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIFDSKIA 368
           GWE+MIPLAFFAGSGVRVANELGAGNGKGAKFAT VAV TSV+IG+FFW++IM FD++I+
Sbjct: 308 GWEMMIPLAFFAGSGVRVANELGAGNGKGAKFATKVAVGTSVVIGVFFWIIIMTFDTQIS 367

Query: 369 LIFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLP 428
           LIF+SS+ VLKEVKKLS+LLAF+ILLNSVQP+LSGVAVGSGWQSYVAYVNLGCYYLIGLP
Sbjct: 368 LIFSSSEVVLKEVKKLSILLAFTILLNSVQPVLSGVAVGSGWQSYVAYVNLGCYYLIGLP 427

Query: 429 LGFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKWAEETP 488
           LG LMG GF QGVMGIW GMIFGGTAIQTLIL IMTIRCDWDKEAEKA + I++W EET 
Sbjct: 428 LGLLMGWGFKQGVMGIWGGMIFGGTAIQTLILCIMTIRCDWDKEAEKARLQIREWGEETG 487

Query: 489 EH 490
            H
Sbjct: 488 GH 487

BLAST of MC10g0533 vs. NCBI nr
Match: XP_023516283.1 (protein DETOXIFICATION 27-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 816 bits (2107), Expect = 2.33e-295
Identity = 418/482 (86.72%), Postives = 450/482 (93.36%), Query Frame = 0

Query: 9   EEANVPLLQPKSQIQEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFA 68
           +E NVPLLQ KS IQE+  DD PLSTR+  ESRKLWHIVGPA+FSRVASYSMLVITQAFA
Sbjct: 8   QEPNVPLLQSKSLIQEE--DDAPLSTRIRVESRKLWHIVGPAVFSRVASYSMLVITQAFA 67

Query: 69  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVL 128
           GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKK HMLGIYMQRSWIVL
Sbjct: 68  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVL 127

Query: 129 FICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLK 188
           FICCIFLLPIYLFATPALKLLGQP+DLAEL+GKV++LL+PLHFSFAFQFPLQRFLQSQLK
Sbjct: 128 FICCIFLLPIYLFATPALKLLGQPADLAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLK 187

Query: 189 TAAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLT 248
           TA IAYVSLVAL+VH+LVSW  V GLKLGL GTA+TIN SWWVLVFGLLIYTVSGGCP T
Sbjct: 188 TAVIAYVSLVALLVHILVSWWFVSGLKLGLFGTAITINISWWVLVFGLLIYTVSGGCPHT 247

Query: 249 WTGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTIN 308
           WTGFS+E FSGLW+FVKLSAASGLMLCLENWYYRILIVM GNL+NAKIAVDALSVCMTIN
Sbjct: 248 WTGFSIETFSGLWDFVKLSAASGLMLCLENWYYRILIVMTGNLDNAKIAVDALSVCMTIN 307

Query: 309 GWELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIFDSKIA 368
           GWE+MIPLAFFAGSGVRVANELGAGNGKGAKFAT VAV TS++IG+FFW++IM FD++I+
Sbjct: 308 GWEMMIPLAFFAGSGVRVANELGAGNGKGAKFATKVAVGTSMVIGVFFWIIIMTFDTQIS 367

Query: 369 LIFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLP 428
           LIF+SS+ VLKEVKKLS+LLAF+ILLNSVQP+LSGVAVGSGWQSYVAYVNLGCYYLIGLP
Sbjct: 368 LIFSSSEVVLKEVKKLSILLAFTILLNSVQPVLSGVAVGSGWQSYVAYVNLGCYYLIGLP 427

Query: 429 LGFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKWAEETP 488
           LG LMG GF QGVMGIW GMIFGGTAIQTLIL IMTIRCDWDKEAEKA + I++W EET 
Sbjct: 428 LGLLMGWGFKQGVMGIWGGMIFGGTAIQTLILCIMTIRCDWDKEAEKARLQIREWGEETG 487

Query: 489 EH 490
            H
Sbjct: 488 GH 487

BLAST of MC10g0533 vs. NCBI nr
Match: KAG6600668.1 (Protein DETOXIFICATION 27, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 815 bits (2106), Expect = 3.30e-295
Identity = 418/482 (86.72%), Postives = 450/482 (93.36%), Query Frame = 0

Query: 9   EEANVPLLQPKSQIQEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFA 68
           +E NVPLLQ KS IQE+  DD PLSTR+  ESRKLWHIVGPA+FSRVASYSMLVITQAFA
Sbjct: 8   QEPNVPLLQSKSLIQEE--DDAPLSTRIRVESRKLWHIVGPAVFSRVASYSMLVITQAFA 67

Query: 69  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVL 128
           GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKK HMLGIYMQRSWIVL
Sbjct: 68  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVL 127

Query: 129 FICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLK 188
           FICCIFLLPIYLFATPALKLLGQP+DLAEL+GKV++LL+PLHFSFAFQFPLQRFLQSQLK
Sbjct: 128 FICCIFLLPIYLFATPALKLLGQPADLAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLK 187

Query: 189 TAAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLT 248
           TA IAYVSLVAL+VH+LVSW  V GLKLGL GTA+TIN SWWVLVFGLLIYTVSGGCP T
Sbjct: 188 TAVIAYVSLVALLVHILVSWWFVSGLKLGLFGTAITINISWWVLVFGLLIYTVSGGCPHT 247

Query: 249 WTGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTIN 308
           WTGFS+E FSGLW+FVKLSAASGLMLCLENWYYRILIVM GNL+NAKIAVDALSVCMTIN
Sbjct: 248 WTGFSIETFSGLWDFVKLSAASGLMLCLENWYYRILIVMTGNLDNAKIAVDALSVCMTIN 307

Query: 309 GWELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIFDSKIA 368
           GWE+MIPLAFFAGSGVRVANELGAGNGKGAKFAT VAV TSV+IG+FFW++IM FD++I+
Sbjct: 308 GWEMMIPLAFFAGSGVRVANELGAGNGKGAKFATKVAVGTSVVIGVFFWIIIMTFDTQIS 367

Query: 369 LIFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLP 428
           LIF+SS+ VLKEVKKLS+LLAF+ILLNSVQP+LSGVAVGSGWQSYVAYVNLGCYYLIGLP
Sbjct: 368 LIFSSSEVVLKEVKKLSILLAFTILLNSVQPVLSGVAVGSGWQSYVAYVNLGCYYLIGLP 427

Query: 429 LGFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKWAEETP 488
           LG LMG GF QGVMGIW GMIFGGTAIQTLIL IMTIRCDWDKEAEKA + I++W EE+ 
Sbjct: 428 LGLLMGWGFKQGVMGIWGGMIFGGTAIQTLILCIMTIRCDWDKEAEKARLQIREWGEESG 487

Query: 489 EH 490
            H
Sbjct: 488 GH 487

BLAST of MC10g0533 vs. NCBI nr
Match: XP_022999771.1 (protein DETOXIFICATION 27-like [Cucurbita maxima])

HSP 1 Score: 812 bits (2098), Expect = 5.46e-294
Identity = 415/479 (86.64%), Postives = 449/479 (93.74%), Query Frame = 0

Query: 9   EEANVPLLQPKSQIQEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFA 68
           +E NVPLLQ KS IQE+  DD PLSTR+  ESRKLWHIVGPA+FSRVASYSMLVITQAFA
Sbjct: 8   QEPNVPLLQSKSLIQEE--DDAPLSTRIRVESRKLWHIVGPAVFSRVASYSMLVITQAFA 67

Query: 69  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVL 128
           GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKK HMLGIYMQRSWIVL
Sbjct: 68  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVL 127

Query: 129 FICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLK 188
           FICCIFLLPIYLFATPALKLLGQP+DLAEL+GKV++LL+PLHFSFAFQFPLQRFLQSQLK
Sbjct: 128 FICCIFLLPIYLFATPALKLLGQPADLAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLK 187

Query: 189 TAAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLT 248
           TA IAYVSLVAL+VH+LVSW  V GLKLGL GTA+TIN SWWVLVFGLLIYTVSGGCP T
Sbjct: 188 TAVIAYVSLVALLVHILVSWWFVSGLKLGLFGTAITINISWWVLVFGLLIYTVSGGCPHT 247

Query: 249 WTGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTIN 308
           WTGFS+E FSGLW+FVKLSAASGLMLCLENWYYRILIVM GNL+NAKIAVDALSVCMTIN
Sbjct: 248 WTGFSIETFSGLWDFVKLSAASGLMLCLENWYYRILIVMTGNLDNAKIAVDALSVCMTIN 307

Query: 309 GWELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIFDSKIA 368
           GWE+MIPLAFFAGSGVRVANELGAGNGKGAKFAT VAV TSV+IG+FFW++IM FD++I+
Sbjct: 308 GWEMMIPLAFFAGSGVRVANELGAGNGKGAKFATKVAVGTSVVIGVFFWIIIMTFDTQIS 367

Query: 369 LIFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLP 428
           LIF+SS+ VLKEVKKLS+LLAF+ILLNSVQP+LSGVAVGSGWQSYVAYVNLGCYYLIGLP
Sbjct: 368 LIFSSSEVVLKEVKKLSILLAFTILLNSVQPVLSGVAVGSGWQSYVAYVNLGCYYLIGLP 427

Query: 429 LGFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKWAEET 487
           LG LMG GF QGVMGIW GMIFGGTAIQT+IL I+TIRCDWDKEAEKA + I++W E+T
Sbjct: 428 LGLLMGWGFKQGVMGIWGGMIFGGTAIQTMILCIITIRCDWDKEAEKARLQIREWGEDT 484

BLAST of MC10g0533 vs. NCBI nr
Match: KAG7031307.1 (Protein DETOXIFICATION 27, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 808 bits (2087), Expect = 3.49e-292
Identity = 418/490 (85.31%), Postives = 450/490 (91.84%), Query Frame = 0

Query: 9   EEANVPLLQPKSQIQEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFA 68
           +E NVPLLQ KS IQE+  DD PLSTR+  ESRKLWHIVGPA+FSRVASYSMLVITQAFA
Sbjct: 8   QEPNVPLLQSKSLIQEE--DDAPLSTRIRVESRKLWHIVGPAVFSRVASYSMLVITQAFA 67

Query: 69  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVL 128
           GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKK HMLGIYMQRSWIVL
Sbjct: 68  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVL 127

Query: 129 FICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLK 188
           FICCIFLLPIYLFATPALKLLGQP+DLAEL+GKV++LL+PLHFSFAFQFPLQRFLQSQLK
Sbjct: 128 FICCIFLLPIYLFATPALKLLGQPADLAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLK 187

Query: 189 TAAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLT 248
           TA IAYVSLVAL+VH+LVSW  V GLKLGL GTA+TIN SWWVLVFGLLIYTVSGGCP T
Sbjct: 188 TAVIAYVSLVALLVHILVSWWFVSGLKLGLFGTAITINISWWVLVFGLLIYTVSGGCPHT 247

Query: 249 WTGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTIN 308
           WTGFS+E FSGLW+FVKLSAASGLMLCLENWYYRILIVM GNL+NAKIAVDALSVCMTIN
Sbjct: 248 WTGFSIETFSGLWDFVKLSAASGLMLCLENWYYRILIVMTGNLDNAKIAVDALSVCMTIN 307

Query: 309 GWELMIPLAFFAGSG--------VRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLI 368
           GWE+MIPLAFFAGSG        VRVANELGAGNGKGAKFAT VAV TSV+IG+FFW++I
Sbjct: 308 GWEMMIPLAFFAGSGFRIFDEYRVRVANELGAGNGKGAKFATKVAVGTSVVIGVFFWIII 367

Query: 369 MIFDSKIALIFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLG 428
           M FD++I+LIF+SS+ VLKEVKKLS+LLAF+ILLNSVQP+LSGVAVGSGWQSYVAYVNLG
Sbjct: 368 MTFDTQISLIFSSSEVVLKEVKKLSILLAFTILLNSVQPVLSGVAVGSGWQSYVAYVNLG 427

Query: 429 CYYLIGLPLGFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHI 488
           CYYLIGLPLG LMG GF QGVMGIW GMIFGGTAIQTLIL IMTIRCDWDKEAEKA + I
Sbjct: 428 CYYLIGLPLGLLMGWGFKQGVMGIWGGMIFGGTAIQTLILCIMTIRCDWDKEAEKARLQI 487

Query: 489 KKWAEETPEH 490
           ++W EE+  H
Sbjct: 488 REWGEESGGH 495

BLAST of MC10g0533 vs. ExPASy TrEMBL
Match: A0A6J1FUM6 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111447024 PE=3 SV=1)

HSP 1 Score: 816 bits (2107), Expect = 1.13e-295
Identity = 418/482 (86.72%), Postives = 450/482 (93.36%), Query Frame = 0

Query: 9   EEANVPLLQPKSQIQEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFA 68
           +E NVPLLQ KS IQE+  DD PLSTR+  ESRKLWHIVGPA+FSRVASYSMLVITQAFA
Sbjct: 8   QEPNVPLLQSKSLIQEE--DDAPLSTRIRVESRKLWHIVGPAVFSRVASYSMLVITQAFA 67

Query: 69  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVL 128
           GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKK HMLGIYMQRSWIVL
Sbjct: 68  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVL 127

Query: 129 FICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLK 188
           FICCIFLLPIYLFATPALKLLGQP+DLAEL+GKV++LL+PLHFSFAFQFPLQRFLQSQLK
Sbjct: 128 FICCIFLLPIYLFATPALKLLGQPADLAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLK 187

Query: 189 TAAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLT 248
           TA IAYVSLVAL+VH+LVSW  V GLKLGL GTA+T+N SWWVLVFGLLIYTVSGGCP T
Sbjct: 188 TAVIAYVSLVALLVHILVSWWFVSGLKLGLFGTAITMNISWWVLVFGLLIYTVSGGCPHT 247

Query: 249 WTGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTIN 308
           WTGFS+E FSGLW+FVKLSAASGLMLCLENWYYRILIVM GNL+NAKIAVDALSVCMTIN
Sbjct: 248 WTGFSIETFSGLWDFVKLSAASGLMLCLENWYYRILIVMTGNLDNAKIAVDALSVCMTIN 307

Query: 309 GWELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIFDSKIA 368
           GWE+MIPLAFFAGSGVRVANELGAGNGKGAKFAT VAV TSV+IG+FFW++IM FD++I+
Sbjct: 308 GWEMMIPLAFFAGSGVRVANELGAGNGKGAKFATKVAVGTSVVIGVFFWIIIMTFDTQIS 367

Query: 369 LIFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLP 428
           LIF+SS+ VLKEVKKLS+LLAF+ILLNSVQP+LSGVAVGSGWQSYVAYVNLGCYYLIGLP
Sbjct: 368 LIFSSSEVVLKEVKKLSILLAFTILLNSVQPVLSGVAVGSGWQSYVAYVNLGCYYLIGLP 427

Query: 429 LGFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKWAEETP 488
           LG LMG GF QGVMGIW GMIFGGTAIQTLIL IMTIRCDWDKEAEKA + I++W EET 
Sbjct: 428 LGLLMGWGFKQGVMGIWGGMIFGGTAIQTLILCIMTIRCDWDKEAEKARLQIREWGEETG 487

Query: 489 EH 490
            H
Sbjct: 488 GH 487

BLAST of MC10g0533 vs. ExPASy TrEMBL
Match: A0A6J1KE20 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111494018 PE=3 SV=1)

HSP 1 Score: 812 bits (2098), Expect = 2.64e-294
Identity = 415/479 (86.64%), Postives = 449/479 (93.74%), Query Frame = 0

Query: 9   EEANVPLLQPKSQIQEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFA 68
           +E NVPLLQ KS IQE+  DD PLSTR+  ESRKLWHIVGPA+FSRVASYSMLVITQAFA
Sbjct: 8   QEPNVPLLQSKSLIQEE--DDAPLSTRIRVESRKLWHIVGPAVFSRVASYSMLVITQAFA 67

Query: 69  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVL 128
           GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKK HMLGIYMQRSWIVL
Sbjct: 68  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVL 127

Query: 129 FICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLK 188
           FICCIFLLPIYLFATPALKLLGQP+DLAEL+GKV++LL+PLHFSFAFQFPLQRFLQSQLK
Sbjct: 128 FICCIFLLPIYLFATPALKLLGQPADLAELAGKVAVLLVPLHFSFAFQFPLQRFLQSQLK 187

Query: 189 TAAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLT 248
           TA IAYVSLVAL+VH+LVSW  V GLKLGL GTA+TIN SWWVLVFGLLIYTVSGGCP T
Sbjct: 188 TAVIAYVSLVALLVHILVSWWFVSGLKLGLFGTAITINISWWVLVFGLLIYTVSGGCPHT 247

Query: 249 WTGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTIN 308
           WTGFS+E FSGLW+FVKLSAASGLMLCLENWYYRILIVM GNL+NAKIAVDALSVCMTIN
Sbjct: 248 WTGFSIETFSGLWDFVKLSAASGLMLCLENWYYRILIVMTGNLDNAKIAVDALSVCMTIN 307

Query: 309 GWELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIFDSKIA 368
           GWE+MIPLAFFAGSGVRVANELGAGNGKGAKFAT VAV TSV+IG+FFW++IM FD++I+
Sbjct: 308 GWEMMIPLAFFAGSGVRVANELGAGNGKGAKFATKVAVGTSVVIGVFFWIIIMTFDTQIS 367

Query: 369 LIFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLP 428
           LIF+SS+ VLKEVKKLS+LLAF+ILLNSVQP+LSGVAVGSGWQSYVAYVNLGCYYLIGLP
Sbjct: 368 LIFSSSEVVLKEVKKLSILLAFTILLNSVQPVLSGVAVGSGWQSYVAYVNLGCYYLIGLP 427

Query: 429 LGFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKWAEET 487
           LG LMG GF QGVMGIW GMIFGGTAIQT+IL I+TIRCDWDKEAEKA + I++W E+T
Sbjct: 428 LGLLMGWGFKQGVMGIWGGMIFGGTAIQTMILCIITIRCDWDKEAEKARLQIREWGEDT 484

BLAST of MC10g0533 vs. ExPASy TrEMBL
Match: A0A0A0KZ65 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_4G099750 PE=3 SV=1)

HSP 1 Score: 796 bits (2057), Expect = 3.98e-288
Identity = 406/482 (84.23%), Postives = 442/482 (91.70%), Query Frame = 0

Query: 10  EANVPLLQPKSQI-QEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFA 69
           E+NVPLL+ K QI +E   +   LSTR+W ES++LWHIVGPAIFSRVASYSMLVITQAFA
Sbjct: 3   ESNVPLLELKPQIIEEDDANGSSLSTRIWVESKQLWHIVGPAIFSRVASYSMLVITQAFA 62

Query: 70  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVL 129
           GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKK HMLGIYMQRSWIVL
Sbjct: 63  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVL 122

Query: 130 FICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLK 189
           FIC I +LPIYLF+TP LKLLGQPSDLAE++GKV+M+ +PLHFSFA QFPLQRFLQSQLK
Sbjct: 123 FICSILILPIYLFSTPVLKLLGQPSDLAEMAGKVAMVFLPLHFSFAIQFPLQRFLQSQLK 182

Query: 190 TAAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLT 249
           TA IAY+SLVALVVH+LVSWL VYGLKLGLVGTA+T N SWWVLVFGLL YT+ GGCP T
Sbjct: 183 TAVIAYLSLVALVVHILVSWLFVYGLKLGLVGTAITTNISWWVLVFGLLFYTICGGCPGT 242

Query: 250 WTGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTIN 309
           W GFS EAFSGLWEFVKLS ASG+M+CLENWYYRILIVM GNL NAK+AVDALSVCMTIN
Sbjct: 243 WCGFSFEAFSGLWEFVKLSTASGVMICLENWYYRILIVMTGNLANAKLAVDALSVCMTIN 302

Query: 310 GWELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIFDSKIA 369
           GWE+MIPLAFF GSGVRVANELGAGNGKGAKFATMVAV TS+IIG+FFW++I+ FDS+IA
Sbjct: 303 GWEMMIPLAFFVGSGVRVANELGAGNGKGAKFATMVAVGTSIIIGIFFWIIIITFDSQIA 362

Query: 370 LIFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLP 429
           LIFTSS+ VLKEVK L++LLAF+ILLNSVQP+LSGVAVGSGWQSYVAYVNLGCYYLIGLP
Sbjct: 363 LIFTSSEVVLKEVKTLTILLAFTILLNSVQPVLSGVAVGSGWQSYVAYVNLGCYYLIGLP 422

Query: 430 LGFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKWAEETP 489
           LGFLMG GFN GV GIWAGMIFGGTAIQTLIL IMTIRCDW+KEAE+AS+ IKKW EETP
Sbjct: 423 LGFLMGWGFNLGVKGIWAGMIFGGTAIQTLILCIMTIRCDWNKEAERASVQIKKWEEETP 482

BLAST of MC10g0533 vs. ExPASy TrEMBL
Match: A0A6J1ESE5 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111436076 PE=3 SV=1)

HSP 1 Score: 789 bits (2038), Expect = 3.23e-285
Identity = 404/487 (82.96%), Postives = 445/487 (91.38%), Query Frame = 0

Query: 1   MSSNLTDQEEANVPLLQPKSQIQEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSM 60
           M++N+ +QE + VPLLQ K +    ++ +  L TR+W ES+KLWHIVGPAIFSR+ASYSM
Sbjct: 1   MATNIIEQE-STVPLLQSKPK----HNGNGALPTRIWVESQKLWHIVGPAIFSRLASYSM 60

Query: 61  LVITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIY 120
           LVITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKK HMLGIY
Sbjct: 61  LVITQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIY 120

Query: 121 MQRSWIVLFICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQ 180
           MQRSWIVLFIC + +LPIYLFATPAL LLGQP DLAEL+GKV+ LL+PLHFSFA QFP+Q
Sbjct: 121 MQRSWIVLFICSVLILPIYLFATPALLLLGQPPDLAELAGKVAALLVPLHFSFAIQFPVQ 180

Query: 181 RFLQSQLKTAAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYT 240
           RFLQSQLKTA IAYVSLVALVVHVLVSWLLVY L+LGLVGTA+T N SWWVLV GL  Y 
Sbjct: 181 RFLQSQLKTAVIAYVSLVALVVHVLVSWLLVYNLELGLVGTAITTNISWWVLVLGLFFYA 240

Query: 241 VSGGCPLTWTGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDA 300
           V GGCPLTW GFSVEAFSGLWEFVKLSAASG+MLCLENWYYRILIVM GNLENAK+AVDA
Sbjct: 241 VCGGCPLTWPGFSVEAFSGLWEFVKLSAASGIMLCLENWYYRILIVMTGNLENAKLAVDA 300

Query: 301 LSVCMTINGWELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLI 360
           LSVCMTINGWE+MIP AFFAGSGVRVANELGAGNGKGA+FATMVAV TSV+IGLFFW++I
Sbjct: 301 LSVCMTINGWEMMIPFAFFAGSGVRVANELGAGNGKGARFATMVAVGTSVVIGLFFWIII 360

Query: 361 MIFDSKIALIFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLG 420
           + FD++IALIFTSS+ VLKEV KLS+LLAF+ILLNS+QP+LSGVAVGSGWQSYVAYVNLG
Sbjct: 361 ITFDTQIALIFTSSQVVLKEVNKLSILLAFTILLNSIQPVLSGVAVGSGWQSYVAYVNLG 420

Query: 421 CYYLIGLPLGFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHI 480
           CYYLIGLPLG +MG GFNQGVMGIWAGMIFGGTAIQTL+L IMTIRCDWD+EAE+ S+HI
Sbjct: 421 CYYLIGLPLGIVMGWGFNQGVMGIWAGMIFGGTAIQTLVLCIMTIRCDWDREAERTSLHI 480

Query: 481 KKWAEET 487
           +K AEET
Sbjct: 481 RKLAEET 482

BLAST of MC10g0533 vs. ExPASy TrEMBL
Match: A0A1S4E0N8 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103495737 PE=3 SV=1)

HSP 1 Score: 786 bits (2030), Expect = 5.14e-284
Identity = 399/482 (82.78%), Postives = 440/482 (91.29%), Query Frame = 0

Query: 10  EANVPLLQPKSQI-QEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFA 69
           E+NVPLL+ K QI +E   +   LSTR+W ES++LWHIVGPAIFSRVASYSMLVITQAFA
Sbjct: 3   ESNVPLLESKPQIIEEDDANGSSLSTRIWVESKQLWHIVGPAIFSRVASYSMLVITQAFA 62

Query: 70  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVL 129
           GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKK HMLGIYMQRSWIVL
Sbjct: 63  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKFHMLGIYMQRSWIVL 122

Query: 130 FICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLK 189
           FICCI  LPIYLF+TP LKLLGQPSDLAE++GKV+++ +PLHFSFA QFPLQRFLQSQLK
Sbjct: 123 FICCILTLPIYLFSTPFLKLLGQPSDLAEMAGKVAVMFLPLHFSFAIQFPLQRFLQSQLK 182

Query: 190 TAAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLT 249
           TA IAYVSLVALVVH+LVSWL VYGL+LGLVGTA+T N SWWVLV GL  YT+ GGCP T
Sbjct: 183 TAVIAYVSLVALVVHILVSWLFVYGLELGLVGTAITTNISWWVLVLGLSFYTICGGCPRT 242

Query: 250 WTGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTIN 309
           W+GFSVEAFSGLWEF KLS ASG+M+CLENWYYRILIVM GNL NAK+AVDALSVCMTIN
Sbjct: 243 WSGFSVEAFSGLWEFAKLSTASGVMICLENWYYRILIVMTGNLANAKLAVDALSVCMTIN 302

Query: 310 GWELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIFDSKIA 369
           GWE+MIPLAFF GSGVRVANELGAGNGKGAKFATMVAV TS++IG+FFW++I+ FDS+IA
Sbjct: 303 GWEMMIPLAFFVGSGVRVANELGAGNGKGAKFATMVAVGTSIVIGIFFWMIIITFDSQIA 362

Query: 370 LIFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLP 429
           LIFTSS+ +L EVK L++LLAF+ILLNSVQ +LSGVAVGSGWQSYVAYVNLGCYYLIGLP
Sbjct: 363 LIFTSSEVILNEVKTLTMLLAFTILLNSVQLVLSGVAVGSGWQSYVAYVNLGCYYLIGLP 422

Query: 430 LGFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKWAEETP 489
           LG LMG GFN GV GIWAGMIFGGTAIQTLIL +MTIRCDW+KEAE+AS++IKKW EETP
Sbjct: 423 LGSLMGWGFNLGVKGIWAGMIFGGTAIQTLILCLMTIRCDWNKEAERASLYIKKWEEETP 482

BLAST of MC10g0533 vs. TAIR 10
Match: AT5G65380.1 (MATE efflux family protein )

HSP 1 Score: 684.1 bits (1764), Expect = 8.2e-197
Identity = 345/475 (72.63%), Postives = 401/475 (84.42%), Query Frame = 0

Query: 10  EANVPLLQPKSQIQEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFAG 69
           E+ V LL+     +E   D E L  R+  E++KLW IVGPAIFSRV +YSMLVITQAFAG
Sbjct: 11  ESRVALLKSPHTAEE---DGEGLKDRILVETKKLWQIVGPAIFSRVTTYSMLVITQAFAG 70

Query: 70  HLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVLF 129
           HLGDLELAA+SI NNV VGF+FGLLLGMASALETLCGQA+GAKK HMLG+YMQRSWIVLF
Sbjct: 71  HLGDLELAAISIVNNVTVGFNFGLLLGMASALETLCGQAFGAKKYHMLGVYMQRSWIVLF 130

Query: 130 ICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLKT 189
            CC+ LLP Y+F TP LK LGQP D+AELSG V++ +IPLHF+F   FPLQRFLQ QLK 
Sbjct: 131 FCCVLLLPTYIFTTPVLKFLGQPDDIAELSGVVAIWVIPLHFAFTLSFPLQRFLQCQLKN 190

Query: 190 AAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLTW 249
              AY + VALVVH+LV WL V GLKLG+VGT  TI+ SWWV V  LL+Y+  GGCPLTW
Sbjct: 191 RVTAYAAAVALVVHILVCWLFVDGLKLGVVGTVATISISWWVNVLILLVYSTCGGCPLTW 250

Query: 250 TGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTING 309
           TG S EA +GLWEF+KLSA+SG+MLCLENWYYRILI+M GNL+NA+IAVD+LS+CM ING
Sbjct: 251 TGLSSEALTGLWEFLKLSASSGVMLCLENWYYRILIIMTGNLQNARIAVDSLSICMAING 310

Query: 310 WELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIFDSKIAL 369
           WE+MIPLAFFAG+GVRVANELGAGNGKGA+FAT+V+V  S+IIGLFFWVLIM+  ++IA 
Sbjct: 311 WEMMIPLAFFAGTGVRVANELGAGNGKGARFATIVSVTQSLIIGLFFWVLIMLLHNQIAW 370

Query: 370 IFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLPL 429
           IF+SS AVL  V KLS+LLAF++LLNSVQP+LSGVAVGSGWQSYVAY+NLGCYY IG+PL
Sbjct: 371 IFSSSVAVLDAVNKLSLLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYCIGVPL 430

Query: 430 GFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKWA 485
           GFLMG GF  GVMGIW GMIFGGTA+QT+ILS +T+RCDW+KEA+KAS  I KW+
Sbjct: 431 GFLMGWGFKLGVMGIWGGMIFGGTAVQTMILSFITMRCDWEKEAQKASARINKWS 482

BLAST of MC10g0533 vs. TAIR 10
Match: AT5G10420.1 (MATE efflux family protein )

HSP 1 Score: 632.9 bits (1631), Expect = 2.2e-181
Identity = 315/475 (66.32%), Postives = 392/475 (82.53%), Query Frame = 0

Query: 9   EEANVPLLQPKSQIQEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFA 68
           EEA VPLL+  +  +E       +   +W E++K+W+IVGP+IF+ +A+YS+L+ITQAFA
Sbjct: 12  EEATVPLLECHNAAEE----GGGMKREIWIETKKIWYIVGPSIFTGLATYSILIITQAFA 71

Query: 69  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVL 128
           GHLGDLELAA+SI NN  +GF++GLLLGMASALETLCGQA+GA++ +MLG+YMQR WI+L
Sbjct: 72  GHLGDLELAAISIINNFTLGFNYGLLLGMASALETLCGQAFGAREYYMLGVYMQRYWIIL 131

Query: 129 FICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLK 188
           F+CCI LLP+YLFATP LK +GQ  D+AEL+G +++ +IP+HF+FAF FPL RFLQ QLK
Sbjct: 132 FLCCILLLPMYLFATPILKFIGQSDDIAELTGTIALWVIPVHFAFAFFFPLNRFLQCQLK 191

Query: 189 TAAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLT 248
              IA  + V+L VH+LV W  VYG KLG++GT  ++N  WW+ +F L +Y+  GGC LT
Sbjct: 192 NKVIAISAGVSLAVHILVCWFFVYGYKLGIIGTMASVNVPWWLNIFILFLYSTRGGCTLT 251

Query: 249 WTGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTIN 308
           WTGFS EAF+GL E  KLSA+SG+MLCLENWYY+IL++M GNL NAKIAVD+LS+CM++N
Sbjct: 252 WTGFSSEAFTGLLELTKLSASSGIMLCLENWYYKILMLMTGNLVNAKIAVDSLSICMSVN 311

Query: 309 GWELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIFDSKIA 368
           GWE+MIPLAFFAG+GVRVANELGAGNGKGA+FAT+V++  S++IGLFF V+I+IF  +I 
Sbjct: 312 GWEMMIPLAFFAGTGVRVANELGAGNGKGARFATIVSITLSLMIGLFFTVIIVIFHDQIG 371

Query: 369 LIFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLP 428
            IF+SS+AVL  V  LSVLLAF++LLNSVQP+LSGVAVGSGWQSYVAY+NLGCYYLIGLP
Sbjct: 372 SIFSSSEAVLNAVDNLSVLLAFTVLLNSVQPVLSGVAVGSGWQSYVAYINLGCYYLIGLP 431

Query: 429 LGFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKW 484
            G  MG  F  GV GIWAGMIFGGTAIQTLIL I+T RCDWD EA K+S+ IKKW
Sbjct: 432 FGLTMGWIFKFGVKGIWAGMIFGGTAIQTLILIIITTRCDWDNEAHKSSVRIKKW 482

BLAST of MC10g0533 vs. TAIR 10
Match: AT5G44050.1 (MATE efflux family protein )

HSP 1 Score: 598.2 bits (1541), Expect = 6.0e-171
Identity = 300/476 (63.03%), Postives = 381/476 (80.04%), Query Frame = 0

Query: 9   EEANVPLLQPKSQIQEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFA 68
           E+A +PLL+ ++  +E   ++  +   +W E++KLW IVGPAIF+RV +  + VITQAFA
Sbjct: 13  EKAKIPLLKDQNVAEE---ENGEIKKEIWLETKKLWRIVGPAIFTRVTTNLIFVITQAFA 72

Query: 69  GHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVL 128
           GHLG+LELAA+SI NNVI+GF++ L +GMA+ALETLCGQA+GAKK  M G+Y+QRSWIVL
Sbjct: 73  GHLGELELAAISIVNNVIIGFNYSLFIGMATALETLCGQAFGAKKYDMFGVYLQRSWIVL 132

Query: 129 FICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLK 188
           F+  I LLP+Y+FATP LK +GQP D+AELSG +S+  IP HFSFAF FP+ RFLQ QLK
Sbjct: 133 FLFSILLLPMYIFATPILKFMGQPDDIAELSGIISVWAIPTHFSFAFFFPINRFLQCQLK 192

Query: 189 TAAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLT 248
            + IA  S V+LVVH+ V WL VY L+LG++GT  T N SWW+ VF L  YT  GGCPLT
Sbjct: 193 NSVIAISSGVSLVVHIFVCWLFVYVLELGVIGTIATANVSWWLNVFILFTYTTCGGCPLT 252

Query: 249 WTGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTIN 308
           WTGFS+E+F+ LWEF KLSA+SG+M+CLENWYYR+LIVM GNLE+A+I VD++S+CM+IN
Sbjct: 253 WTGFSMESFTRLWEFTKLSASSGIMVCLENWYYRMLIVMTGNLEDARIDVDSMSICMSIN 312

Query: 309 GWELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIFDSKIA 368
           G E+M+PLAFFAG+ VRVANELGAGNGK A+FA +++V  S+IIG+   VLI     +I 
Sbjct: 313 GLEMMVPLAFFAGTSVRVANELGAGNGKRARFAMIISVTQSLIIGIIISVLIYFLLDQIG 372

Query: 369 LIFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLP 428
            +F+SS+ VLK V  LS+LL+F+ILLNSVQP+LSGVAVGSGWQS VA++NLGCYY IGLP
Sbjct: 373 WMFSSSETVLKAVNNLSILLSFAILLNSVQPVLSGVAVGSGWQSLVAFINLGCYYFIGLP 432

Query: 429 LGFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKWA 485
           LG +MG  F  GV GIWAGMIFGGT +QTLIL  +T+RCDW+KEA+ A + + KW+
Sbjct: 433 LGIVMGWMFKFGVKGIWAGMIFGGTMVQTLILIFITMRCDWEKEAQNAKVRVNKWS 485

BLAST of MC10g0533 vs. TAIR 10
Match: AT1G33110.1 (MATE efflux family protein )

HSP 1 Score: 449.1 bits (1154), Expect = 4.5e-126
Identity = 233/474 (49.16%), Postives = 327/474 (68.99%), Query Frame = 0

Query: 10  EANVPLLQPKSQIQEQYHDDEPLSTRVWAESRKLWHIVGPAIFSRVASYSMLVITQAFAG 69
           E    LL+  ++   +  D+  L  +VW ES+KLW +  PAIF+R +++ + +I+Q+F G
Sbjct: 7   ELTAALLKKTAENGGEEKDELGLKQKVWIESKKLWIVAAPAIFTRFSTFGVSIISQSFIG 66

Query: 70  HLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQRSWIVLF 129
           HLG +ELAA SI   V++ F  G+LLGMASALETLCGQAYGAK+ HMLGIY+QRSWIVL 
Sbjct: 67  HLGPIELAAYSITFTVLLRFSNGILLGMASALETLCGQAYGAKQNHMLGIYLQRSWIVLT 126

Query: 130 ICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFLQSQLKT 189
            C I L P+Y+F+ P L  LGQ   +  ++  +++ +I ++FSF   F  Q FLQ+Q K 
Sbjct: 127 GCTICLTPVYIFSGPILLALGQEERIVRVARIIALWVIGINFSFVPSFTCQMFLQAQSKN 186

Query: 190 AAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSGGCPLTW 249
             IAYV+ V+L VHV +SWLL+     G+ G   +   ++W+     L++   GGC  TW
Sbjct: 187 KIIAYVAAVSLGVHVFLSWLLMVHFNFGITGAMTSTLVAFWLPNIAQLLFVTCGGCKDTW 246

Query: 250 TGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSVCMTING 309
            GFS+ AF  LW   KLS +SG MLCLE WY  IL+++ GNL+NA++A+DAL++C+ ING
Sbjct: 247 RGFSMMAFKDLWPVFKLSMSSGGMLCLELWYNSILVLLTGNLKNAEVALDALAICLNING 306

Query: 310 WELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIFDSKIAL 369
            E+MI L F A + VRV+NELG+GN KGAKFAT+ AV TS+ +G+  + + +    +++ 
Sbjct: 307 LEMMIALGFLAAASVRVSNELGSGNPKGAKFATLTAVFTSLSLGIVLFFVFLFLRGRVSY 366

Query: 370 IFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYYLIGLPL 429
           IFT+S+AV  EV  LS LLAFSIL+NSVQP+LSGVAVG+GWQ YV YVNL CYYL+G+P+
Sbjct: 367 IFTTSEAVAAEVADLSPLLAFSILMNSVQPVLSGVAVGAGWQGYVTYVNLACYYLVGIPI 426

Query: 430 GFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKW 484
           G ++G      V G+W GM+F G  +QT +L++MT+R DWD++   +   + +W
Sbjct: 427 GIILGYVVGLQVKGVWIGMLF-GIFVQTCVLTVMTLRTDWDQQVSTSLRRLNRW 479

BLAST of MC10g0533 vs. TAIR 10
Match: AT1G47530.1 (MATE efflux family protein )

HSP 1 Score: 447.6 bits (1150), Expect = 1.3e-125
Identity = 233/486 (47.94%), Postives = 332/486 (68.31%), Query Frame = 0

Query: 9   EEANVPLLQPKSQIQEQYHDDEPLSTRVWA-----ESRKLWHIVGPAIFSRVASYSMLVI 68
           ++  +PLL P+   +         +++VWA     ES++LW + GPAIF+ ++ YS+  +
Sbjct: 3   KDKTLPLLDPR---EPPELTGTKSASKVWAKEFGEESKRLWELAGPAIFTAISQYSLGAL 62

Query: 69  TQAFAGHLGDLELAAMSIANNVIVGFDFGLLLGMASALETLCGQAYGAKKLHMLGIYMQR 128
           TQ F+G LG+LELAA+S+ N+VI G  FG++LGM SALETLCGQAYGA ++ M+GIYMQR
Sbjct: 63  TQTFSGRLGELELAAVSVENSVISGLAFGVMLGMGSALETLCGQAYGAGQIRMMGIYMQR 122

Query: 129 SWIVLFICCIFLLPIYLFATPALKLLGQPSDLAELSGKVSMLLIPLHFSFAFQFPLQRFL 188
           SW++LF   +FLLP+Y++A P L   G+   +++ +GK ++ +IP  F++A  FP+Q+FL
Sbjct: 123 SWVILFTTALFLLPVYIWAPPILSFFGEAPHISKAAGKFALWMIPQLFAYAANFPIQKFL 182

Query: 189 QSQLKTAAIAYVSLVALVVHVLVSWLLVYGLKLGLVGTAVTINFSWWVLVFGLLIYTVSG 248
           QSQ K   +A++S V LV+H + SWL +   K GLVG A+T+N SWW++V G L+Y +  
Sbjct: 183 QSQRKVLVMAWISGVVLVIHAVFSWLFILYFKWGLVGAAITLNTSWWLIVIGQLLYILIT 242

Query: 249 GCPLTWTGFSVEAFSGLWEFVKLSAASGLMLCLENWYYRILIVMAGNLENAKIAVDALSV 308
                WTGFS+ AF  L+ FVKLS AS LMLCLE WY  +L+V+ G L N  I VDA+S+
Sbjct: 243 KSDGAWTGFSMLAFRDLYGFVKLSLASALMLCLEFWYLMVLVVVTGLLPNPLIPVDAISI 302

Query: 309 CMTINGWELMIPLAFFAGSGVRVANELGAGNGKGAKFATMVAVVTSVIIGLFFWVLIMIF 368
           CM I GW  MI + F A   VRV+NELGAGN   AKF+ +V  +TS +IG+   ++++  
Sbjct: 303 CMNIEGWTAMISIGFNAAISVRVSNELGAGNAALAKFSVIVVSITSTLIGIVCMIVVLAT 362

Query: 369 DSKIALIFTSSKAVLKEVKKLSVLLAFSILLNSVQPILSGVAVGSGWQSYVAYVNLGCYY 428
                 +FTSS+AV  E  +++VLL F++LLNS+QP+LSGVAVG+GWQ+ VAYVN+ CYY
Sbjct: 363 KDSFPYLFTSSEAVAAETTRIAVLLGFTVLLNSLQPVLSGVAVGAGWQALVAYVNIACYY 422

Query: 429 LIGLPLGFLMGRGFNQGVMGIWAGMIFGGTAIQTLILSIMTIRCDWDKEAEKASMHIKKW 488
           +IGLP G ++G   + GV GIW GM+  G  +QTLIL  +    +W+KEAE+A   +++W
Sbjct: 423 IIGLPAGLVLGFTLDLGVQGIWGGMV-AGICLQTLILIGIIYFTNWNKEAEQAESRVQRW 482

Query: 489 AEETPE 490
                E
Sbjct: 483 GGTAQE 484