MC10g0527 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g0527
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	1-aminocyclopropane-1-carboxylate synthase
Location	MC10: 4369219 .. 4370868 (-)
RNA-Seq Expression	MC10g0527
Synteny	MC10g0527

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGCTGTTGTCTAGAAAAGCCACGTGCAACTCTCATGGCCAAGATTCCTCCTACTTCCTGGGCTGGGAGGAATACGAGAAGAATCCCTACGACGAGATTCGAAATCCTGGAGGGATTATTCAGATGGGTCTTGCAGAGAATCAGGTGCCTGGCTATAACATTAATTTCAGCTCCAATTATACATCTACGTACCTGATGGAAGTAATAATGCTAATTAACGGTCCTCGTTTGACTTTCATTTTGGCAGCTATGTTTTGACTTGCTCGAGAAATGGCTGGAGGAGAACCCGGACGCGGCTGGCTTCAAGAGAGACGGCAAGTCAATTTTCAGAGAACTCGCCCTCTTCCAAGACTACAACGGCCTCCCCGCATTCAAAAAGGTACACATCCATTCCATATACACCCTTCTTTAATTTAACTGTATATATTTCATGTAATTAAATTAATAAAGAAATAAATGTTTCAGGCATTGGCAGAATTCATGTCGGAAATCCGAGGGAACAAAGTAAGGTTCGATCCCAACCACATAGTCCTCACTGCCGGCGCCACGTCAGCCAACGAGACTCTTATGTTCTGCTTGGCCGAACCTGACGATGCATTCCTCCTGCCTACCCCATACTATCCAGGGTGAGTCTATCGATATTCATATGATTCCATCACAAAATTAGAGAGGTAGGGTATTAAATAAACAGATATTCTGTTGGACAGATTCGATAGAGATTTGAAATGGAGGACTGGGATCGAGATCGTGCCGATCCACTGCTCGAGCGACAACGGGTTCCGAATCACGGAGGCGGCGCTGTTACAGGCCTACCAAAACGCGCAGGATCGGCATCTGAAGGTGAAAGGCGTGCTCGTAACGAACCCGTCAAATCCGCTGGGGACCACAATCAGCAGGCAAGAATTAAGCCTTTTACTCCGCTTCGTCGCGACAAAAGACCTCCATCTAATCAGCGACGAGATCTACTCGGGCACCGTTTTTGGCTCCCCCGGCTTCGTGAGCGCGATGGAAGTACTGAACCAAAAGTACAAAAACGACGCCCAAGTCCGGAAGCGAGTTCACATAGTTTACAGCCTGTCCAAAGACCTCGGCCTTCCGGGGTTCCGCGTGGGGGCCATTTACTCAAACGACGAAGTAGTGGTGGCCGCCGCGACGAAGATGTCGAGCTTCGGGCTGGTGTCGTCGCAGACGCAGTACCTGCTGTCGGCGATGCTTTCGGACGAGAAGTTCAGGGGGAGTTACATTAGGGAGAATCAGAGGAGGCTGTTGAGAAGGCAGAGGATGCTGGTGAGTGGGCTGGAGAAGGGCGGGATAAAGTGCCACAAAGGGAATGCGGGGCTGTTCTGTTGGGTGGACATGAGGCATTTGTTGGGGGAGCAGACGTTTGAGGCCGAGATGGAGCTGTGGAGGAAGATTGTGTTCGAGGTTGGGCTCAACGTGTCGCCCGGACAGTCGTGCCATTGCTCGGAGCCGGGGTGGTTCCGCGTCTGCTTCGCCAACATGTCGGAAGCCACGCTGGAAGTGGCCATGCGCCGGTTGAAGGACTTCTTCGTCGCCACCAAAACAAACGCCAACGTTAGGAAGTCGGTAGTTAAATGGGTTTTTCGGTTATCGGTTCAGGATCGCACCGGCGAGCCACGTCACCGA

mRNA sequence

ATGGGGCTGTTGTCTAGAAAAGCCACGTGCAACTCTCATGGCCAAGATTCCTCCTACTTCCTGGGCTGGGAGGAATACGAGAAGAATCCCTACGACGAGATTCGAAATCCTGGAGGGATTATTCAGATGGGTCTTGCAGAGAATCAGCTATGTTTTGACTTGCTCGAGAAATGGCTGGAGGAGAACCCGGACGCGGCTGGCTTCAAGAGAGACGGCAAGTCAATTTTCAGAGAACTCGCCCTCTTCCAAGACTACAACGGCCTCCCCGCATTCAAAAAGGCATTGGCAGAATTCATGTCGGAAATCCGAGGGAACAAAGTAAGGTTCGATCCCAACCACATAGTCCTCACTGCCGGCGCCACGTCAGCCAACGAGACTCTTATGTTCTGCTTGGCCGAACCTGACGATGCATTCCTCCTGCCTACCCCATACTATCCAGGATTCGATAGAGATTTGAAATGGAGGACTGGGATCGAGATCGTGCCGATCCACTGCTCGAGCGACAACGGGTTCCGAATCACGGAGGCGGCGCTGTTACAGGCCTACCAAAACGCGCAGGATCGGCATCTGAAGGTGAAAGGCGTGCTCGTAACGAACCCGTCAAATCCGCTGGGGACCACAATCAGCAGGCAAGAATTAAGCCTTTTACTCCGCTTCGTCGCGACAAAAGACCTCCATCTAATCAGCGACGAGATCTACTCGGGCACCGTTTTTGGCTCCCCCGGCTTCGTGAGCGCGATGGAAGTACTGAACCAAAAGTACAAAAACGACGCCCAAGTCCGGAAGCGAGTTCACATAGTTTACAGCCTGTCCAAAGACCTCGGCCTTCCGGGGTTCCGCGTGGGGGCCATTTACTCAAACGACGAAGTAGTGGTGGCCGCCGCGACGAAGATGTCGAGCTTCGGGCTGGTGTCGTCGCAGACGCAGTACCTGCTGTCGGCGATGCTTTCGGACGAGAAGTTCAGGGGGAGTTACATTAGGGAGAATCAGAGGAGGCTGTTGAGAAGGCAGAGGATGCTGGTGAGTGGGCTGGAGAAGGGCGGGATAAAGTGCCACAAAGGGAATGCGGGGCTGTTCTGTTGGGTGGACATGAGGCATTTGTTGGGGGAGCAGACGTTTGAGGCCGAGATGGAGCTGTGGAGGAAGATTGTGTTCGAGGTTGGGCTCAACGTGTCGCCCGGACAGTCGTGCCATTGCTCGGAGCCGGGGTGGTTCCGCGTCTGCTTCGCCAACATGTCGGAAGCCACGCTGGAAGTGGCCATGCGCCGGTTGAAGGACTTCTTCGTCGCCACCAAAACAAACGCCAACGTTAGGAAGTCGGTAGTTAAATGGGTTTTTCGGTTATCGGTTCAGGATCGCACCGGCGAGCCACGTCACCGA

Coding sequence (CDS)

ATGGGGCTGTTGTCTAGAAAAGCCACGTGCAACTCTCATGGCCAAGATTCCTCCTACTTCCTGGGCTGGGAGGAATACGAGAAGAATCCCTACGACGAGATTCGAAATCCTGGAGGGATTATTCAGATGGGTCTTGCAGAGAATCAGCTATGTTTTGACTTGCTCGAGAAATGGCTGGAGGAGAACCCGGACGCGGCTGGCTTCAAGAGAGACGGCAAGTCAATTTTCAGAGAACTCGCCCTCTTCCAAGACTACAACGGCCTCCCCGCATTCAAAAAGGCATTGGCAGAATTCATGTCGGAAATCCGAGGGAACAAAGTAAGGTTCGATCCCAACCACATAGTCCTCACTGCCGGCGCCACGTCAGCCAACGAGACTCTTATGTTCTGCTTGGCCGAACCTGACGATGCATTCCTCCTGCCTACCCCATACTATCCAGGATTCGATAGAGATTTGAAATGGAGGACTGGGATCGAGATCGTGCCGATCCACTGCTCGAGCGACAACGGGTTCCGAATCACGGAGGCGGCGCTGTTACAGGCCTACCAAAACGCGCAGGATCGGCATCTGAAGGTGAAAGGCGTGCTCGTAACGAACCCGTCAAATCCGCTGGGGACCACAATCAGCAGGCAAGAATTAAGCCTTTTACTCCGCTTCGTCGCGACAAAAGACCTCCATCTAATCAGCGACGAGATCTACTCGGGCACCGTTTTTGGCTCCCCCGGCTTCGTGAGCGCGATGGAAGTACTGAACCAAAAGTACAAAAACGACGCCCAAGTCCGGAAGCGAGTTCACATAGTTTACAGCCTGTCCAAAGACCTCGGCCTTCCGGGGTTCCGCGTGGGGGCCATTTACTCAAACGACGAAGTAGTGGTGGCCGCCGCGACGAAGATGTCGAGCTTCGGGCTGGTGTCGTCGCAGACGCAGTACCTGCTGTCGGCGATGCTTTCGGACGAGAAGTTCAGGGGGAGTTACATTAGGGAGAATCAGAGGAGGCTGTTGAGAAGGCAGAGGATGCTGGTGAGTGGGCTGGAGAAGGGCGGGATAAAGTGCCACAAAGGGAATGCGGGGCTGTTCTGTTGGGTGGACATGAGGCATTTGTTGGGGGAGCAGACGTTTGAGGCCGAGATGGAGCTGTGGAGGAAGATTGTGTTCGAGGTTGGGCTCAACGTGTCGCCCGGACAGTCGTGCCATTGCTCGGAGCCGGGGTGGTTCCGCGTCTGCTTCGCCAACATGTCGGAAGCCACGCTGGAAGTGGCCATGCGCCGGTTGAAGGACTTCTTCGTCGCCACCAAAACAAACGCCAACGTTAGGAAGTCGGTAGTTAAATGGGTTTTTCGGTTATCGGTTCAGGATCGCACCGGCGAGCCACGTCACCGA

Protein sequence

MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLEENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGATSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQAYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGSPGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSSFGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFCWVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVAMRRLKDFFVATKTNANVRKSVVKWVFRLSVQDRTGEPRHR

Homology

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BLAST of MC10g0527 vs. ExPASy Swiss-Prot
Match: Q00257 (1-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita maxima OX=3661 GN=ACS2 PE=2 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 6.8e-206
Identity = 346/466 (74.25%), Postives = 396/466 (84.98%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           M +LS KATCNSHGQDSSYFLGWE YE NP+    NP GIIQMGLAENQL FDLLE WL 
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           +NPDAA FKRDGKSIFRELALFQDY+GLPAFKKAL EFM+EIRGNKV F+ N+IVLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANETLMFCLAE  DAFLLPTPYYPGFDRDLKWRTG+EIVPIHC+S NGF+IT++AL Q
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITQSALEQ 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AY++AQ R+L+VKGVLVTNPSNPLGTT++R EL+L+  F+ +K +HLISDEIYSGTVFGS
Sbjct: 181 AYKDAQTRNLRVKGVLVTNPSNPLGTTMNRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
           PGFVSAMEVL ++   D +V KRVHIVYSLSKDLGLPGFRVGAIYSND++VVAAATKMSS
Sbjct: 241 PGFVSAMEVLKERSSEDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLSD+KF  SYI ENQ+RL +RQ+MLVSGL+K GI C   NAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTISYISENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLL    FE+E+ELW+KIV+EVGLN+SPG SCHC+EPGWFRVCFANMSE+TL++A
Sbjct: 361 WVDMRHLLESDKFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420

Query: 421 MRRLKDFFVATKTNA-------------NVRKSV-VKWVFRLSVQD 453
           +RRLK F    ++               N++K++  KWVFR S Q+
Sbjct: 421 VRRLKSFVTELRSTTTSNHRNHDNKICKNIKKNIFTKWVFRQSAQE 466

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BLAST of MC10g0527 vs. ExPASy Swiss-Prot
Match: Q42881 (1-aminocyclopropane-1-carboxylate synthase 3 OS=Solanum lycopersicum OX=4081 GN=ACS3 PE=1 SV=1)

HSP 1 Score: 717.6 bits (1851), Expect = 8.9e-206
Identity = 348/468 (74.36%), Postives = 392/468 (83.76%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           M LLS KATCNSHGQDSSYFLGW+EYEKNPYDEI+NP GIIQMGLAENQL FDLLE WL 
Sbjct: 1   MKLLSEKATCNSHGQDSSYFLGWQEYEKNPYDEIQNPKGIIQMGLAENQLSFDLLESWLA 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           +NPDAAGFKR+G+SIFRELALFQDY+GLPAFK A+ +FMSEIRGN+V FD N++VLTAGA
Sbjct: 61  QNPDAAGFKRNGESIFRELALFQDYHGLPAFKNAMTKFMSEIRGNRVSFDSNNLVLTAGA 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANETLMFCLA   DAFLLPTPYYPGFDRDLKWRTG EIVPIHCSS NGFRITE+AL +
Sbjct: 121 TSANETLMFCLANQGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFRITESALEE 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AY +A+ R+LKVKGVLVTNPSNPLGTT++R EL LLL F+  K +HLISDEIYSGTVF S
Sbjct: 181 AYLDAKKRNLKVKGVLVTNPSNPLGTTLNRNELELLLTFIDEKGIHLISDEIYSGTVFNS 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
           PGFVS MEVL +K     +V +RVHIVYSLSKDLGLPGFR+GAIYSNDE+VV+AATKMSS
Sbjct: 241 PGFVSVMEVLIEKNYMKTRVWERVHIVYSLSKDLGLPGFRIGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGLVSSQTQYLLS MLSD+KF   YI ENQ+RL +R  MLV GL+  GI C + NAGLFC
Sbjct: 301 FGLVSSQTQYLLSCMLSDKKFTKKYISENQKRLKKRHAMLVKGLKSAGINCLESNAGLFC 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLL    F+AEM+LW+KIV++VGLN+SPG SCHC+EPGWFRVCFANMSE TL++A
Sbjct: 361 WVDMRHLLSSNNFDAEMDLWKKIVYDVGLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 MRRLKDFFVATKTNA------------NVRKSVVKWVFRLSVQDRTGE 457
           MRR+KDF  +T  NA            + +KS  KWVFRLS  DR  E
Sbjct: 421 MRRIKDFVESTAPNATNHQNQQQSNANSKKKSFSKWVFRLSFNDRQRE 468

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BLAST of MC10g0527 vs. ExPASy Swiss-Prot
Match: Q9M2Y8 (1-aminocyclopropane-1-carboxylate synthase 9 OS=Arabidopsis thaliana OX=3702 GN=ACS9 PE=1 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 3.2e-203
Identity = 343/470 (72.98%), Postives = 393/470 (83.62%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           M  LSRK T N+HGQDSSYFLGWEEYEKNPYDEI+NP GIIQMGLAENQLCFDL+E WL 
Sbjct: 1   MKQLSRKVTSNAHGQDSSYFLGWEEYEKNPYDEIKNPNGIIQMGLAENQLCFDLIETWLA 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           +NPDAAG K+DG+SIF+ELALFQDY+GLP FKKALAEFM EIRGN+V FDP+ IVL AG+
Sbjct: 61  KNPDAAGLKKDGQSIFKELALFQDYHGLPEFKKALAEFMEEIRGNRVTFDPSKIVLAAGS 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANETLMFCLAEP DAFLLPTPYYPGFDRDLKWRTG EIVPIHCSS NGF+ITE+AL Q
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AYQ AQ   LKVKGVLVTNPSNPLGT ++R+EL+LL+ F+ +K++HLISDEIYSGTVFG 
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTMLTRRELNLLVDFITSKNIHLISDEIYSGTVFGF 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
             FVS M+VL  K   +++V KRVHIVYSLSKDLGLPGFRVGAIYSNDE+VV+AATKMSS
Sbjct: 241 EQFVSVMDVLKDKNLENSEVSKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSA+LSD+KF  +Y+ ENQ+RL  RQ+ LVSGLE  GI C K NAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSTYLDENQKRLKIRQKKLVSGLEAAGITCLKSNAGLFC 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLL   TFEAE+ELW+KIV++V LN+SPG SCHC+EPGWFRVCFANMSE TL++A
Sbjct: 361 WVDMRHLLDTNTFEAELELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 MRRLKDFFVATKTNANVRKS------------VVKWVFRLSVQDRTGEPR 459
           M+RLK++  +T +   + KS            V  WVFR+S  DR  + R
Sbjct: 421 MKRLKEYVESTDSRRVISKSSHDRIKSLRKRTVSNWVFRVSWTDRVPDER 470

[Close]

BLAST of MC10g0527 vs. ExPASy Swiss-Prot
Match: Q37001 (1-aminocyclopropane-1-carboxylate synthase 5 OS=Arabidopsis thaliana OX=3702 GN=ACS5 PE=1 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 2.5e-200
Identity = 334/470 (71.06%), Postives = 385/470 (81.91%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           M  LS K T N HGQDSSYFLGWEEYEKNPYDEI+NP G+IQMGLAENQLCFDL+E WL 
Sbjct: 1   MKQLSTKVTSNGHGQDSSYFLGWEEYEKNPYDEIKNPNGMIQMGLAENQLCFDLIESWLT 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           +NPDAA  KR+G+SIFRELALFQDY+G+P FKKA+AEFM EIRGN+V FDP  IVL AG+
Sbjct: 61  KNPDAASLKRNGQSIFRELALFQDYHGMPEFKKAMAEFMEEIRGNRVTFDPKKIVLAAGS 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANETLMFCLAEP DAFLLPTPYYPGFDRDLKWRTG EIVPIHCSS NGF+ITE+AL Q
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AYQ AQ   LKVKGVLVTNPSNPLGT ++R+EL+LL+ F+ +K++HLISDEIYSGT+FG 
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTALTRRELNLLVDFITSKNIHLISDEIYSGTMFGF 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
             F+S M+VL  K   D +V KRVH+VYSLSKDLGLPGFRVGAIYSNDE++V+AATKMSS
Sbjct: 241 EQFISVMDVLKDKKLEDTEVSKRVHVVYSLSKDLGLPGFRVGAIYSNDEMIVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSA+LSD+KF   Y+ ENQ+RL  RQR LVSGLE  GI C + NAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSQYLEENQKRLKSRQRRLVSGLESAGITCLRSNAGLFC 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLL   TFEAE++LW+KIV+ V LN+SPG SCHC+EPGWFRVCFANMSE TL++A
Sbjct: 361 WVDMRHLLDTNTFEAELDLWKKIVYNVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 MRRLKDFFVATKTNANV------------RKSVVKWVFRLSVQDRTGEPR 459
           ++RLK F  +T     +            +K+V  WVFR+S  DR  + R
Sbjct: 421 LKRLKTFVESTDCGRMISRSSHERLKSLRKKTVSNWVFRVSWTDRVPDER 470

[Close]

BLAST of MC10g0527 vs. ExPASy Swiss-Prot
Match: P37821 (1-aminocyclopropane-1-carboxylate synthase OS=Malus domestica OX=3750 GN=ACS-1 PE=1 SV=2)

HSP 1 Score: 686.8 bits (1771), Expect = 1.7e-196
Identity = 330/467 (70.66%), Postives = 388/467 (83.08%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           M +LSR AT NSHGQDSSYFLGW+EYEKNPY E+ N  GIIQMGLAENQLCFDLLE WL 
Sbjct: 1   MRMLSRNATFNSHGQDSSYFLGWQEYEKNPYHEVHNTNGIIQMGLAENQLCFDLLESWLA 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           +NP+AA FK++G+SIF ELALFQDY+GLPAFKKA+ +FM+EIRGNKV FDPNH+VLTAGA
Sbjct: 61  KNPEAAAFKKNGESIFAELALFQDYHGLPAFKKAMVDFMAEIRGNKVTFDPNHLVLTAGA 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANET +FCLA+P +A L+PTPYYPGFDRDLKWRTG+EIVPIHC+S NGF+ITE AL +
Sbjct: 121 TSANETFIFCLADPGEAVLIPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITETALEE 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AYQ A+ R+L+VKGVLVTNPSNPLGTT++R EL LLL FV  K +HLISDEIYSGT F S
Sbjct: 181 AYQEAEKRNLRVKGVLVTNPSNPLGTTMTRNELYLLLSFVEDKGIHLISDEIYSGTAFSS 240

Query: 241 PGFVSAMEVL-NQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMS 300
           P F+S MEVL ++    +++V +RVH+VYSLSKDLGLPGFRVGAIYSND++VVAAATKMS
Sbjct: 241 PSFISVMEVLKDRNCDENSEVWQRVHVVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMS 300

Query: 301 SFGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLF 360
           SFGLVSSQTQ+LLSAMLSD+K   +YI EN +RL +RQ+ LVSGL+K GI C  GNAGLF
Sbjct: 301 SFGLVSSQTQHLLSAMLSDKKLTKNYIAENHKRLKQRQKKLVSGLQKSGISCLNGNAGLF 360

Query: 361 CWVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEV 420
           CWVDMRHLL   TFEAEMELW+KIV+EV LN+SPG SCHC+EPGWFRVCFAN+ E TL++
Sbjct: 361 CWVDMRHLLRSNTFEAEMELWKKIVYEVHLNISPGSSCHCTEPGWFRVCFANLPERTLDL 420

Query: 421 AMRRLKDFF-------------VATKTNANVRKSVVKWVFRLSVQDR 454
           AM+RLK F               ++  + + R+S+ KWV RLS  DR
Sbjct: 421 AMQRLKAFVGEYYNVPEVNGGSQSSHLSHSRRQSLTKWVSRLSFDDR 467

[Close]

BLAST of MC10g0527 vs. NCBI nr
Match: XP_022136794.1 (1-aminocyclopropane-1-carboxylate synthase CMA101-like [Momordica charantia])

HSP 1 Score: 936 bits (2420), Expect = 0.0
Identity = 460/460 (100.00%), Postives = 460/460 (100.00%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE
Sbjct: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ
Sbjct: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS
Sbjct: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
           PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS
Sbjct: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA
Sbjct: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420

Query: 421 MRRLKDFFVATKTNANVRKSVVKWVFRLSVQDRTGEPRHR 460
           MRRLKDFFVATKTNANVRKSVVKWVFRLSVQDRTGEPRHR
Sbjct: 421 MRRLKDFFVATKTNANVRKSVVKWVFRLSVQDRTGEPRHR 460

[Close]

BLAST of MC10g0527 vs. NCBI nr
Match: KAG7015411.1 (1-aminocyclopropane-1-carboxylate synthase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 795 bits (2054), Expect = 3.66e-288
Identity = 391/466 (83.91%), Postives = 421/466 (90.34%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNP GIIQMGLAENQL FDL+E+WLE
Sbjct: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           ENP+AAGFKRDGKSIF+ELALFQDYNGLPAFKKALAEFMSEIR NKV FDP+HIVLTAGA
Sbjct: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANETLMFCLAEP DAFLLPTPYYPGFDRDLKWRTGIEIVPI CSS NGF+IT+ ALL+
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AYQ+AQ+R+LKVKGVLVTNPSNPLGTTIS QEL+LLL+F+A+K+LHLISDEIYSGTVF +
Sbjct: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
           P FVS ME+LNQ YK++ Q+RK+VHIVYSLSKDLGLPGFRVGA+YSNDEVVVAA TKMSS
Sbjct: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLSD+ FR  YIRENQ RL +RQRMLV GLEK GIKCHKGNAGLF 
Sbjct: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLLG  TFEAEMELWRKIVFEV LNVSPGQSCHCSEPGWFRVCFANMSEATL++A
Sbjct: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MRRLKDFFVATKTNANV------RKSVVKWVFRLSVQDRTGEPRHR 460
           M+R K F   + TN+        RKSV KWVFRLSV DRT E R R
Sbjct: 421 MKRFKAFVADSTTNSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR 466

[Close]

BLAST of MC10g0527 vs. NCBI nr
Match: XP_022929274.1 (1-aminocyclopropane-1-carboxylate synthase 9-like [Cucurbita moschata])

HSP 1 Score: 795 bits (2053), Expect = 8.47e-288
Identity = 395/479 (82.46%), Postives = 422/479 (88.10%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNP GIIQMGLAENQLCFDL+E+WLE
Sbjct: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLCFDLVERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIF+ELALFQDYNGLPAFKKALAEFMSEIR NKV FDP+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANETLMFCLAEP DAFLLPTPYYPGFDRDLKWRTGIEIVPI CSS NGF+IT+ ALL+
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AYQ+AQ+R+LKVKGVLVTNPSNPLGTTIS QEL+LLL+F+ +K+LHLISDEIYSGTVF +
Sbjct: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIVSKNLHLISDEIYSGTVFQT 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
           P FVS ME+LNQ YK++ Q+RK+VHIVYSLSKDLGLPGFRVGA+YSNDEVVVAAATKMSS
Sbjct: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLSDE FR  YIRENQ RL +RQRMLV GLEK GIKCHKGNAGLF 
Sbjct: 301 FGLVSSQTQYLLSAMLSDETFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLLG  TFEAEMELWRKIVFEV LNVSPGQSCHCSEPGWFRVCFANMSEATL++A
Sbjct: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MRRLKDFFVATKT-----------NANV--------RKSVVKWVFRLSVQDRTGEPRHR 460
           M+R K F   + T           N N         RKSV KWVFRLSV DRT E R R
Sbjct: 421 MKRFKAFVADSTTVKLNSSRIRSRNTNSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR 479

[Close]

BLAST of MC10g0527 vs. NCBI nr
Match: XP_022985152.1 (1-aminocyclopropane-1-carboxylate synthase 9-like [Cucurbita maxima])

HSP 1 Score: 794 bits (2051), Expect = 1.47e-287
Identity = 389/465 (83.66%), Postives = 424/465 (91.18%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           MG+LSRKATCNSHGQDSSYFLGWEEY+KNPYDEI NP GIIQMGLAENQLCFDLLE+WLE
Sbjct: 1   MGILSRKATCNSHGQDSSYFLGWEEYDKNPYDEILNPKGIIQMGLAENQLCFDLLERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGN+V F+P+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNRVTFNPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANETLMFCLAEP DAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSS NGFRITE+ALL+
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFRITESALLE 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AYQ+AQ R LKVKGVLVTNPSNPLGTTI+ QEL+LLL+F+A+K+LHLISDEIYSGTVF +
Sbjct: 181 AYQSAQSRDLKVKGVLVTNPSNPLGTTITTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
           PGFVS ME+LNQ YK++ Q+RK+VHIVYSLSKDLGLPGFRVGAIYSND+VVVAAATKMSS
Sbjct: 241 PGFVSVMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLSD+KF  +YI+ENQ+RL +RQ+MLV GL+K GIKCHKGNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTKNYIKENQKRLEKRQKMLVRGLQKAGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLLG  TFE+EMELWRKIVFEV LNVSPGQSCHCSEPGWFRVCFANMSEATL++A
Sbjct: 361 WVDMRHLLGANTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MRRLKDFFV--------ATKTNAN----VRKSVVKWVFRLSVQDR 453
           M+RLK F V         T T  N     RK++VKWVFRLS  DR
Sbjct: 421 MQRLKAFVVYSTVATSPTTTTTTNHLPTKRKNMVKWVFRLSFNDR 465

[Close]

BLAST of MC10g0527 vs. NCBI nr
Match: XP_022943238.1 (1-aminocyclopropane-1-carboxylate synthase 9-like [Cucurbita moschata])

HSP 1 Score: 794 bits (2051), Expect = 1.47e-287
Identity = 389/465 (83.66%), Postives = 424/465 (91.18%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           MG+LSRKATCNSHGQDSSYFLGWEEY+KNPYDEI NP GIIQMGLAENQLCFDLLE+WLE
Sbjct: 1   MGILSRKATCNSHGQDSSYFLGWEEYDKNPYDEILNPKGIIQMGLAENQLCFDLLERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGN+V F+P+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNRVTFNPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANETLMFCLAEP DAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSS NGFRITE+ALL+
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFRITESALLE 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AYQ+AQ R LKVKGVLVTNPSNPLGTTI+ QEL+LLL+F+A+K+LHLISDEIYSGTVF +
Sbjct: 181 AYQSAQSRDLKVKGVLVTNPSNPLGTTITTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
           PGFVS ME+LNQ YK++ Q+RK+VHIVYSLSKDLGLPGFRVGAIYSND+VVVAAATKMSS
Sbjct: 241 PGFVSVMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLSD+KF  +YI+ENQ+RL +RQ+MLV GL+K GIKCHKGNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTKNYIKENQKRLEKRQKMLVRGLQKAGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLLG  TFE+EMELWRKIVFEV LNVSPGQSCHCSEPGWFRVCFANMSEATL++A
Sbjct: 361 WVDMRHLLGANTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MRRLKDFFV--------ATKTNAN----VRKSVVKWVFRLSVQDR 453
           M+RLK F V         T T  N     RK++VKWVFRLS  DR
Sbjct: 421 MQRLKAFVVDSTVTTSPTTTTTTNHLPTKRKNMVKWVFRLSFNDR 465

[Close]

BLAST of MC10g0527 vs. ExPASy TrEMBL
Match: A0A6J1C5C9 (1-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Momordica charantia OX=3673 GN=LOC111008407 PE=3 SV=1)

HSP 1 Score: 936 bits (2420), Expect = 0.0
Identity = 460/460 (100.00%), Postives = 460/460 (100.00%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE
Sbjct: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ
Sbjct: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS
Sbjct: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
           PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS
Sbjct: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA
Sbjct: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420

Query: 421 MRRLKDFFVATKTNANVRKSVVKWVFRLSVQDRTGEPRHR 460
           MRRLKDFFVATKTNANVRKSVVKWVFRLSVQDRTGEPRHR
Sbjct: 421 MRRLKDFFVATKTNANVRKSVVKWVFRLSVQDRTGEPRHR 460

[Close]

BLAST of MC10g0527 vs. ExPASy TrEMBL
Match: A0A6J1ENA4 (1-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita moschata OX=3662 GN=LOC111435906 PE=3 SV=1)

HSP 1 Score: 795 bits (2053), Expect = 4.10e-288
Identity = 395/479 (82.46%), Postives = 422/479 (88.10%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNP GIIQMGLAENQLCFDL+E+WLE
Sbjct: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLCFDLVERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIF+ELALFQDYNGLPAFKKALAEFMSEIR NKV FDP+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANETLMFCLAEP DAFLLPTPYYPGFDRDLKWRTGIEIVPI CSS NGF+IT+ ALL+
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AYQ+AQ+R+LKVKGVLVTNPSNPLGTTIS QEL+LLL+F+ +K+LHLISDEIYSGTVF +
Sbjct: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIVSKNLHLISDEIYSGTVFQT 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
           P FVS ME+LNQ YK++ Q+RK+VHIVYSLSKDLGLPGFRVGA+YSNDEVVVAAATKMSS
Sbjct: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLSDE FR  YIRENQ RL +RQRMLV GLEK GIKCHKGNAGLF 
Sbjct: 301 FGLVSSQTQYLLSAMLSDETFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLLG  TFEAEMELWRKIVFEV LNVSPGQSCHCSEPGWFRVCFANMSEATL++A
Sbjct: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MRRLKDFFVATKT-----------NANV--------RKSVVKWVFRLSVQDRTGEPRHR 460
           M+R K F   + T           N N         RKSV KWVFRLSV DRT E R R
Sbjct: 421 MKRFKAFVADSTTVKLNSSRIRSRNTNSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR 479

[Close]

BLAST of MC10g0527 vs. ExPASy TrEMBL
Match: A0A6J1J434 (1-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita maxima OX=3661 GN=LOC111483239 PE=3 SV=1)

HSP 1 Score: 794 bits (2051), Expect = 7.12e-288
Identity = 389/465 (83.66%), Postives = 424/465 (91.18%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           MG+LSRKATCNSHGQDSSYFLGWEEY+KNPYDEI NP GIIQMGLAENQLCFDLLE+WLE
Sbjct: 1   MGILSRKATCNSHGQDSSYFLGWEEYDKNPYDEILNPKGIIQMGLAENQLCFDLLERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGN+V F+P+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNRVTFNPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANETLMFCLAEP DAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSS NGFRITE+ALL+
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFRITESALLE 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AYQ+AQ R LKVKGVLVTNPSNPLGTTI+ QEL+LLL+F+A+K+LHLISDEIYSGTVF +
Sbjct: 181 AYQSAQSRDLKVKGVLVTNPSNPLGTTITTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
           PGFVS ME+LNQ YK++ Q+RK+VHIVYSLSKDLGLPGFRVGAIYSND+VVVAAATKMSS
Sbjct: 241 PGFVSVMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLSD+KF  +YI+ENQ+RL +RQ+MLV GL+K GIKCHKGNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTKNYIKENQKRLEKRQKMLVRGLQKAGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLLG  TFE+EMELWRKIVFEV LNVSPGQSCHCSEPGWFRVCFANMSEATL++A
Sbjct: 361 WVDMRHLLGANTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MRRLKDFFV--------ATKTNAN----VRKSVVKWVFRLSVQDR 453
           M+RLK F V         T T  N     RK++VKWVFRLS  DR
Sbjct: 421 MQRLKAFVVYSTVATSPTTTTTTNHLPTKRKNMVKWVFRLSFNDR 465

[Close]

BLAST of MC10g0527 vs. ExPASy TrEMBL
Match: A0A6J1FWL0 (1-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita moschata OX=3662 GN=LOC111448029 PE=3 SV=1)

HSP 1 Score: 794 bits (2051), Expect = 7.12e-288
Identity = 389/465 (83.66%), Postives = 424/465 (91.18%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           MG+LSRKATCNSHGQDSSYFLGWEEY+KNPYDEI NP GIIQMGLAENQLCFDLLE+WLE
Sbjct: 1   MGILSRKATCNSHGQDSSYFLGWEEYDKNPYDEILNPKGIIQMGLAENQLCFDLLERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGN+V F+P+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNRVTFNPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANETLMFCLAEP DAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSS NGFRITE+ALL+
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFRITESALLE 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AYQ+AQ R LKVKGVLVTNPSNPLGTTI+ QEL+LLL+F+A+K+LHLISDEIYSGTVF +
Sbjct: 181 AYQSAQSRDLKVKGVLVTNPSNPLGTTITTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
           PGFVS ME+LNQ YK++ Q+RK+VHIVYSLSKDLGLPGFRVGAIYSND+VVVAAATKMSS
Sbjct: 241 PGFVSVMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSAMLSD+KF  +YI+ENQ+RL +RQ+MLV GL+K GIKCHKGNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTKNYIKENQKRLEKRQKMLVRGLQKAGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLLG  TFE+EMELWRKIVFEV LNVSPGQSCHCSEPGWFRVCFANMSEATL++A
Sbjct: 361 WVDMRHLLGANTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MRRLKDFFV--------ATKTNAN----VRKSVVKWVFRLSVQDR 453
           M+RLK F V         T T  N     RK++VKWVFRLS  DR
Sbjct: 421 MQRLKAFVVDSTVTTSPTTTTTTNHLPTKRKNMVKWVFRLSFNDR 465

[Close]

BLAST of MC10g0527 vs. ExPASy TrEMBL
Match: A0A6J1J417 (1-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita maxima OX=3661 GN=LOC111483220 PE=3 SV=1)

HSP 1 Score: 789 bits (2037), Expect = 1.12e-285
Identity = 393/479 (82.05%), Postives = 422/479 (88.10%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           MGLLS KATCNSHGQDSSYFLGWEEYEKNPY EIRNP GIIQMGLAENQLCFDL+E+WLE
Sbjct: 1   MGLLSTKATCNSHGQDSSYFLGWEEYEKNPYHEIRNPNGIIQMGLAENQLCFDLVERWLE 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           ENPDAAGFKRDGKSIF+ELALFQDYNGLPAFKKALAEFMSEIR NKV FDP+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANETLMFCLAEP DAFLLPTPYYPGFDRDLKWRTGIEIVPI CSS NGF+IT+ ALL+
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AYQ+AQ+R+LKVKGVLVTNPSNPLGTTIS QEL+LLL+F+A+K+LHLISDEIYSGTVF +
Sbjct: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
           P FVS ME+LNQ YK++ Q+RK+VHIVYSLSKDLGLPGFRVGA+YSNDEVVVAAATKMSS
Sbjct: 241 PAFVSVMEILNQSYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGLVSSQTQYLLS MLSDE FR  YIRENQ RL +RQRMLV GLEK GIKCHKGNAGLF 
Sbjct: 301 FGLVSSQTQYLLSVMLSDETFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLLG  TFEAEMELWRKIVFEV LNVSP QSCHCSEPGWFRVCFANMSEATL++A
Sbjct: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPSQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MRRLKDFFV---ATKTNA------NV----------RKSVVKWVFRLSVQDRTGEPRHR 460
           M+R K F V    TK N+      NV          RKSV KWVFRLS+ DR+ E R R
Sbjct: 421 MKRFKAFVVDSTTTKLNSTWIRSRNVNSTSNNLDTKRKSVAKWVFRLSIHDRSSESRDR 479

[Close]

BLAST of MC10g0527 vs. TAIR 10
Match: AT3G49700.1 (1-aminocyclopropane-1-carboxylate synthase 9 )

HSP 1 Score: 709.1 bits (1829), Expect = 2.2e-204
Identity = 343/470 (72.98%), Postives = 393/470 (83.62%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           M  LSRK T N+HGQDSSYFLGWEEYEKNPYDEI+NP GIIQMGLAENQLCFDL+E WL 
Sbjct: 1   MKQLSRKVTSNAHGQDSSYFLGWEEYEKNPYDEIKNPNGIIQMGLAENQLCFDLIETWLA 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           +NPDAAG K+DG+SIF+ELALFQDY+GLP FKKALAEFM EIRGN+V FDP+ IVL AG+
Sbjct: 61  KNPDAAGLKKDGQSIFKELALFQDYHGLPEFKKALAEFMEEIRGNRVTFDPSKIVLAAGS 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANETLMFCLAEP DAFLLPTPYYPGFDRDLKWRTG EIVPIHCSS NGF+ITE+AL Q
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AYQ AQ   LKVKGVLVTNPSNPLGT ++R+EL+LL+ F+ +K++HLISDEIYSGTVFG 
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTMLTRRELNLLVDFITSKNIHLISDEIYSGTVFGF 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
             FVS M+VL  K   +++V KRVHIVYSLSKDLGLPGFRVGAIYSNDE+VV+AATKMSS
Sbjct: 241 EQFVSVMDVLKDKNLENSEVSKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSA+LSD+KF  +Y+ ENQ+RL  RQ+ LVSGLE  GI C K NAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSTYLDENQKRLKIRQKKLVSGLEAAGITCLKSNAGLFC 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLL   TFEAE+ELW+KIV++V LN+SPG SCHC+EPGWFRVCFANMSE TL++A
Sbjct: 361 WVDMRHLLDTNTFEAELELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 MRRLKDFFVATKTNANVRKS------------VVKWVFRLSVQDRTGEPR 459
           M+RLK++  +T +   + KS            V  WVFR+S  DR  + R
Sbjct: 421 MKRLKEYVESTDSRRVISKSSHDRIKSLRKRTVSNWVFRVSWTDRVPDER 470

[Close]

BLAST of MC10g0527 vs. TAIR 10
Match: AT5G65800.1 (ACC synthase 5 )

HSP 1 Score: 699.5 bits (1804), Expect = 1.8e-201
Identity = 334/470 (71.06%), Postives = 385/470 (81.91%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           M  LS K T N HGQDSSYFLGWEEYEKNPYDEI+NP G+IQMGLAENQLCFDL+E WL 
Sbjct: 1   MKQLSTKVTSNGHGQDSSYFLGWEEYEKNPYDEIKNPNGMIQMGLAENQLCFDLIESWLT 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           +NPDAA  KR+G+SIFRELALFQDY+G+P FKKA+AEFM EIRGN+V FDP  IVL AG+
Sbjct: 61  KNPDAASLKRNGQSIFRELALFQDYHGMPEFKKAMAEFMEEIRGNRVTFDPKKIVLAAGS 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           TSANETLMFCLAEP DAFLLPTPYYPGFDRDLKWRTG EIVPIHCSS NGF+ITE+AL Q
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AYQ AQ   LKVKGVLVTNPSNPLGT ++R+EL+LL+ F+ +K++HLISDEIYSGT+FG 
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTALTRRELNLLVDFITSKNIHLISDEIYSGTMFGF 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
             F+S M+VL  K   D +V KRVH+VYSLSKDLGLPGFRVGAIYSNDE++V+AATKMSS
Sbjct: 241 EQFISVMDVLKDKKLEDTEVSKRVHVVYSLSKDLGLPGFRVGAIYSNDEMIVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGLVSSQTQYLLSA+LSD+KF   Y+ ENQ+RL  RQR LVSGLE  GI C + NAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSQYLEENQKRLKSRQRRLVSGLESAGITCLRSNAGLFC 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLL   TFEAE++LW+KIV+ V LN+SPG SCHC+EPGWFRVCFANMSE TL++A
Sbjct: 361 WVDMRHLLDTNTFEAELDLWKKIVYNVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 MRRLKDFFVATKTNANV------------RKSVVKWVFRLSVQDRTGEPR 459
           ++RLK F  +T     +            +K+V  WVFR+S  DR  + R
Sbjct: 421 LKRLKTFVESTDCGRMISRSSHERLKSLRKKTVSNWVFRVSWTDRVPDER 470

[Close]

BLAST of MC10g0527 vs. TAIR 10
Match: AT4G37770.1 (1-amino-cyclopropane-1-carboxylate synthase 8 )

HSP 1 Score: 683.3 bits (1762), Expect = 1.3e-196
Identity = 332/469 (70.79%), Postives = 381/469 (81.24%), Query Frame = 0


Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60
           MGLLS+KA+CN+HGQDSSYF GWEEYEKNPYDEI+NP GIIQMGLAENQL FDL+E WL 
Sbjct: 1   MGLLSKKASCNTHGQDSSYFWGWEEYEKNPYDEIKNPDGIIQMGLAENQLSFDLIESWLA 60

Query: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120
           +NPDAA F+R+G+SIFRELALFQDY+GLP+FK A+A+FMSE RGN+V F+PN +VLTAGA
Sbjct: 61  KNPDAANFQREGQSIFRELALFQDYHGLPSFKNAMADFMSENRGNRVSFNPNKLVLTAGA 120

Query: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180
           T ANETLMFCLA+P DAFLLPTPYYPGFDRDLKWRTG EIVPI C S NGFRIT+ AL +
Sbjct: 121 TPANETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIQCKSANGFRITKVALEE 180

Query: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240
           AY+ AQ  +LKVKGVL+TNPSNPLGTT +R EL+ LL F++ K +HLISDEIYSGTVF +
Sbjct: 181 AYEQAQKLNLKVKGVLITNPSNPLGTTTTRTELNHLLDFISRKKIHLISDEIYSGTVFTN 240

Query: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300
           PGF+S MEVL  +   +  V  RVHIVYSLSKDLGLPGFRVG IYSND+ VV+AATKMSS
Sbjct: 241 PGFISVMEVLKDRKLENTDVFDRVHIVYSLSKDLGLPGFRVGVIYSNDDFVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360
           FGL+SSQTQYLLSA+LSD+ F  +Y+ ENQ RL  R + LVSGLE  GI+C K NAGLFC
Sbjct: 301 FGLISSQTQYLLSALLSDKTFTKNYLEENQIRLKNRHKKLVSGLEAAGIECLKSNAGLFC 360

Query: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420
           WVDMRHLL   TFEAE+ELW+KIV+EV LN+SPG SCHC+EPGWFRVCFAN+SE TL+VA
Sbjct: 361 WVDMRHLLKSNTFEAEIELWKKIVYEVKLNISPGSSCHCNEPGWFRVCFANLSEETLKVA 420

Query: 421 MRRLKDFFVATKTN----------ANVRK-SVVKWVFRLSVQDRTGEPR 459
           + RLK F                  N+RK  V  WVFRLS  DR  E R
Sbjct: 421 LDRLKRFVDGPSPTRRSQSEHQRLKNLRKMKVSNWVFRLSFHDREPEER 469

[Close]

BLAST of MC10g0527 vs. TAIR 10
Match: AT2G22810.1 (1-aminocyclopropane-1-carboxylate synthase 4 )

HSP 1 Score: 656.8 bits (1693), Expect = 1.3e-188
Identity = 322/471 (68.37%), Postives = 373/471 (79.19%), Query Frame = 0


Query: 4   LSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLEENP 63
           LSRKATCNSHGQ SSYFLGWEEYEKNPYD  +NP GIIQMGLAENQLCFDLLE WL +N 
Sbjct: 4   LSRKATCNSHGQVSSYFLGWEEYEKNPYDVTKNPQGIIQMGLAENQLCFDLLESWLAQNT 63

Query: 64  DAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGATSA 123
           DAA FKRDG+S+FRELALFQDY+GL +FK A A+FMSE RGN+V FD N++VLTAGATSA
Sbjct: 64  DAACFKRDGQSVFRELALFQDYHGLSSFKNAFADFMSENRGNRVSFDSNNLVLTAGATSA 123

Query: 124 NETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQAYQ 183
           NETLMFCLA+P DAFLLPTPYYPGFDRDLKWRTG+EIVPI  SS NGFRIT+ AL +AY+
Sbjct: 124 NETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGVEIVPIQSSSTNGFRITKLALEEAYE 183

Query: 184 NAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVA-TKDLHLISDEIYSGTVFGSPG 243
            A+   L VKG+L+TNPSNPLGTT ++ EL++L  F+   K++HL+SDEIYSGTVF S  
Sbjct: 184 QAKKLDLNVKGILITNPSNPLGTTTTQTELNILFDFITKNKNIHLVSDEIYSGTVFNSSE 243

Query: 244 FVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSSFG 303
           F+S ME+L      +  V  RVHIV SLSKDLGLPGFRVGAIYSND+ V++AATKMSSFG
Sbjct: 244 FISVMEILKNNQLENTDVLNRVHIVCSLSKDLGLPGFRVGAIYSNDKDVISAATKMSSFG 303

Query: 304 LVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFCWV 363
           LVSSQTQYLLS++LSD+KF  +Y+RENQ+RL  RQR LV GLE  GIKC K NAGLFCWV
Sbjct: 304 LVSSQTQYLLSSLLSDKKFTKNYLRENQKRLKNRQRKLVLGLEAIGIKCLKSNAGLFCWV 363

Query: 364 DMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVAMR 423
           DMR LL  +TFEAEM+LW+KIV+EV LN+SPG SCHC EPGWFRVCFANM + TL++A++
Sbjct: 364 DMRPLLRSKTFEAEMDLWKKIVYEVKLNISPGSSCHCEEPGWFRVCFANMIDETLKLALK 423

Query: 424 RLKDFFVATKTNANVRKS---------------VVKWVFRLSVQDRTGEPR 459
           RLK       ++   +KS               V  WVFRLS  DR  E R
Sbjct: 424 RLKMLVDDENSSRRCQKSKSERLNGSRKKTMSNVSNWVFRLSFHDREAEER 474

[Close]

BLAST of MC10g0527 vs. TAIR 10
Match: AT4G08040.1 (1-aminocyclopropane-1-carboxylate synthase 11 )

HSP 1 Score: 619.4 bits (1596), Expect = 2.3e-177
Identity = 296/455 (65.05%), Postives = 358/455 (78.68%), Query Frame = 0


Query: 3   LLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLEEN 62
           +LS K   +SHGQDSSYFLGW+EYEKNP+ E  N  GI+QMGLAENQL FDL+EKWLEE+
Sbjct: 1   MLSSKVVGDSHGQDSSYFLGWQEYEKNPFHESFNTSGIVQMGLAENQLSFDLIEKWLEEH 60

Query: 63  PDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGATS 122
           P+  G K++ +S+FR+LALFQDY+GLPAFK A+A+FM +IR NKV+FD N +VLTAG+TS
Sbjct: 61  PEVLGLKKNDESVFRQLALFQDYHGLPAFKDAMAKFMGKIRENKVKFDTNKMVLTAGSTS 120

Query: 123 ANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQAY 182
           ANETLMFCLA P DAFL+P PYYPGFDRDLKWRTG+EIVPIHC S NG++ITE AL  AY
Sbjct: 121 ANETLMFCLANPGDAFLIPAPYYPGFDRDLKWRTGVEIVPIHCVSSNGYKITEDALEDAY 180

Query: 183 QNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGSPG 242
           + A   +L VKGVL+TNPSNPLGT+ +R+EL LLL F +TK +H++SDEIYSGTVF SP 
Sbjct: 181 ERALKHNLNVKGVLITNPSNPLGTSTTREELDLLLTFTSTKKIHMVSDEIYSGTVFDSPE 240

Query: 243 FVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSSFG 302
           F S +EV   K   +  +  ++H+VYSLSKDLGLPGFRVG IYSN+E VV+AATKMSSFG
Sbjct: 241 FTSVLEVAKDK---NMGLDGKIHVVYSLSKDLGLPGFRVGLIYSNNEKVVSAATKMSSFG 300

Query: 303 LVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFCWV 362
           L+SSQTQ+LL+ +LSDE+F  +Y+ EN++RL  R+  LVSGL++ GI C K NAGLFCWV
Sbjct: 301 LISSQTQHLLANLLSDERFTTNYLEENKKRLRERKDRLVSGLKEAGISCLKSNAGLFCWV 360

Query: 363 DMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVAMR 422
           D+RHLL   TFEAE  LW KIV EVGLN+SPG SCHC EPGWFRVCFANMS+ T+EVAM 
Sbjct: 361 DLRHLLKSNTFEAEHSLWTKIVCEVGLNISPGSSCHCDEPGWFRVCFANMSDQTMEVAMD 420

Query: 423 RLKDFF--------VATKTNANVRKSVVKWVFRLS 450
           R+K F           T  +   R  + KWV +LS
Sbjct: 421 RVKGFVDNNNGGKQKRTMWDTRRRSLINKWVSKLS 452

The following BLAST results are available for this feature:

BLAST of MC10g0527 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q00257	6.8e-206	74.25	1-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita maxima OX=3661 GN...	[more]
Q42881	8.9e-206	74.36	1-aminocyclopropane-1-carboxylate synthase 3 OS=Solanum lycopersicum OX=4081 GN=...	[more]
Q9M2Y8	3.2e-203	72.98	1-aminocyclopropane-1-carboxylate synthase 9 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q37001	2.5e-200	71.06	1-aminocyclopropane-1-carboxylate synthase 5 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
P37821	1.7e-196	70.66	1-aminocyclopropane-1-carboxylate synthase OS=Malus domestica OX=3750 GN=ACS-1 P...	[more]

BLAST of MC10g0527 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136794.1	0.0	100.00	1-aminocyclopropane-1-carboxylate synthase CMA101-like [Momordica charantia]	[more]
KAG7015411.1	3.66e-288	83.91	1-aminocyclopropane-1-carboxylate synthase 3, partial [Cucurbita argyrosperma su...	[more]
XP_022929274.1	8.47e-288	82.46	1-aminocyclopropane-1-carboxylate synthase 9-like [Cucurbita moschata]	[more]
XP_022985152.1	1.47e-287	83.66	1-aminocyclopropane-1-carboxylate synthase 9-like [Cucurbita maxima]	[more]
XP_022943238.1	1.47e-287	83.66	1-aminocyclopropane-1-carboxylate synthase 9-like [Cucurbita moschata]	[more]

BLAST of MC10g0527 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C5C9	0.0	100.00	1-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Momordica charantia OX...	[more]
A0A6J1ENA4	4.10e-288	82.46	1-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1J434	7.12e-288	83.66	1-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6J1FWL0	7.12e-288	83.66	1-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1J417	1.12e-285	82.05	1-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita maxima OX=3661 GN...	[more]

BLAST of MC10g0527 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G49700.1	2.2e-204	72.98	1-aminocyclopropane-1-carboxylate synthase 9	[more]
AT5G65800.1	1.8e-201	71.06	ACC synthase 5	[more]
AT4G37770.1	1.3e-196	70.79	1-amino-cyclopropane-1-carboxylate synthase 8	[more]
AT2G22810.1	1.3e-188	68.37	1-aminocyclopropane-1-carboxylate synthase 4	[more]
AT4G08040.1	2.3e-177	65.05	1-aminocyclopropane-1-carboxylate synthase 11	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00753	ACCSYNTHASE	coord: 43..69 score: 64.18 coord: 113..133 score: 66.9 coord: 76..92 score: 68.66 coord: 135..156 score: 81.82 coord: 263..287 score: 83.28 coord: 189..213 score: 64.79 coord: 295..318 score: 81.76 coord: 225..248 score: 63.66
None	No IPR available	PANTHER	PTHR43795:SF10	1-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE 9	coord: 1..434
None	No IPR available	PANTHER	PTHR43795	BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATED	coord: 1..434
None	No IPR available	CDD	cd00609	AAT_like	coord: 77..426 e-value: 2.46744E-64 score: 209.505
IPR015422	Pyridoxal phosphate-dependent transferase, small domain	GENE3D	3.90.1150.10	Aspartate Aminotransferase, domain 1	coord: 29..423 e-value: 7.3E-168 score: 560.3
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 63..318 e-value: 7.3E-168 score: 560.3
IPR004839	Aminotransferase, class I/classII	PFAM	PF00155	Aminotran_1_2	coord: 41..424 e-value: 2.1E-99 score: 333.1
IPR004838	Aminotransferases, class-I, pyridoxal-phosphate-binding site	PROSITE	PS00105	AA_TRANSFER_CLASS_1	coord: 269..282
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 8..427

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g0527.1	MC10g0527.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042218	1-aminocyclopropane-1-carboxylate biosynthetic process
biological_process	GO:0009058	biosynthetic process
molecular_function	GO:0016847	1-aminocyclopropane-1-carboxylate synthase activity
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0008483	transaminase activity
molecular_function	GO:0003824	catalytic activity