MC10g0502 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0502
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionmechanosensitive ion channel protein 2, chloroplastic-like
LocationMC10: 4099653 .. 4107553 (-)
RNA-Seq ExpressionMC10g0502
SyntenyMC10g0502
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGCCCTGTTTTCATTTCTTTTTCTTCCTACCTACGCATGAGCAGGCGAAATAGAGACGGTGGTTTCGATCGGAAGCTGTAGAAATTTGGCCGCCAACGTATTTTGCTTCGATTTGACCAGGTAGTATCTTCGGCGTTCAATCCAAAAATTTCCGTTAATGGCAAATCAGCAAACTTTGGCGTCCGTCTTTTTTTTTTTTTTTTTGGCTTTAGTAGTTTCTCTTTTCCATCGTTGTCATCGTCGTCATCACGGGCAATAACCTTAGAAGTTCTTCAATTACTTAACTTGTGATCAATATCAGGTTAGTATTTGTGTCGATGGATTCAGCTCAGAAGTTTGAAGTTTTGTTTCCTTGGTTCTACCGTTTGATTTTTTTTCTTCTTTATAAGTTGTTGTTTGTTGAATTCTGATCCGCGATTAAGTGTTGTTCTGAAGTTAGGGTTCGGAGAAAAATATGTTATGATTTTGGTTGTGTTATTGCAAGTTATATGAACAAAATGCCAGAGAGAGTTTAGATTCTTGACTATTAATTACTGTTAGTTGGTTCTGTATCACCTATGTTTTCATTCATGTATTAATAAAGCATGATTTAGTGCAGCTTTCAGCTGTCATCGTGCTGCATAGGAGTTTTATTGTTCAAGTGTCAACATCTCAGCATTTATTTAGTTTGAGAAATGGTTATTGTCGGTTCTTTGCAACTGTCCTATCATCTGGGACCCTGGAGGAACCGTTTGTATGAAGAAAACCTTAAGGTATAGGAACCTTCTCCCTGCCTCCAGACAGTTTATATATTTATAAGACTCAAGTAAATATTTTTGTTGTAATGTCTCTTTTTGTTCTTATCCAAAACTTAATGTTCTTTACAGTTTGCCACTCAGAGCAATAACATACGGTTATTGAGTGCAGCCAGTCCATCGCCTCGTTTAGTGAGTTTGTTAGCTTGGAACAATTATTTTTAGCTTTTCTTAAAAAGCATAATTTTAGCCCATCGAAATGCATGTCTGTCAGACGTTGGTGCTACTTGGTTGTCTCTTAGATACGTGCTATACTTCTGAGTCCTCAAATACAAAAGAGTCTGCAATCAGTCCCTTCTTTTTTTTTTTTTTGTTTCAAATTGGTTCCTAAATTGATTATAATTTTTCATTTTGCCAACCTTTTGTTGCTTTGTTGTTGACATGATGTTTCTCAATATGTTTTCTTCAATGAATAACGATAAATTTTGATGATTAAAAATTTTTTGTTTTGTTTTATTACTATTCTTTTGAAACCAGAACTTAAAAAAAAAACTGTTGTCCTTGGAGGTCAGCAAGAATGTGTCCCTTATATCAAAATGGGTTTGGATGCTTTCCATGTGTATTTGGGTGTGTATATTTTGTGTAAGCCTGGCTATTTGAGCATTGGCTGCATATGAAATTCTTGCCTTGTAAATACTTGAAAATATTTCTTATTGCAGTTTCAGCAGAAGAACACTTGGAGCACTCATTTATTTAGCATGAAGTACTCACCTAATTACACTGTACCTTTTAGATACAATGTATTTAGGTGCCACTCGTCATTGATGACAGACCAACCACTGGATCCTCCTGGGATTAAGGCAGCTGTAGTGGCTTTGACAAGGTCAGTATTTGTCTGGTTTCATGTATGAATGCCCCAAAATCTTCCCAGCATTGACTTATATAAGATTGTTCTCACATAATCACATTCACAGTGAAGATCAACGATATTCTCTCCATCCCTTTCACAATTCTGATATGTTACCTGTTAGCTCCACTATTGACACCAAAGCTACTACAAACCTTATAAAAAAAATCACGCCATCTTGATAAATTTTACGTTAGTATAATTTTTTATTCCCAAATATTGGTTCACTTGTATACCATTTCAAAATGTTCCAGTTAGTCCCCAGTTACAAAACATCTTTGTAGGTTTTTGCATCAATAAAAGTAGGTAAAATTTCATTAGAACTTAAGATGGACATATCATTGCATACATGGATGATGTTGGATGTCATTTGAGACAAGTTGAACGAAGGACAATTCTGGAACTTTGAGAAAACACTAGAATTGTGGGTTTTGGTTCAGGAGTACTTTTCTAAAAATACTTGATTAAGTCTCTCATCCCTGGTTTTGATAAATGACTTCTGGTTGCATTTCTCACATATGTCTTCATCCTGAATCTTGGTCTTGCTGACAATTTTGGTGGCCGTCTTCATCCATTACAAGATTGAGTTCCTTGTCATCCTAGCTAGATGTTTCTCCTTTACGTCTAGTGTCAGTTTCTAATTTTTTTAATTGGGACATGACTATAAAGTCAAGTTGTGCTTGAAATCAATCAACATCCAAGACTCAATTTGAGCTTTATCTAGGACCTCGTGTTATGAACTATGTATTCCTTTTAGGAGTAAATTTTAATGCACAGGTAGCCAACCACGCTTGTTTCAAAGTAAGCAATTAGTTGAGCCATCTTAGCTTCGTTAAAACATGCTAGCACAGTTGATGGTTGGGACTAACATGGAGATTTGATTAAAAGCTAGAAACTTGTGTGCTTGTATCATAAAAACTAAAATAGAAAATAGCCAATAGTTTAGGGAACAAATTGTAGTTTAACCTCATATTTTTCAACTGGTGAAATTAATCTTGAGGACTTCAGTCCTTACATGGCGTTCGTTATGGATATAATAATTGCAATGATTCGTACGCTATAGCTGTTGCAGTGTTGTTGGTTTGACTAAATACCAATTTTCTTACTAGTTCAAGTATTTTTTCAAATTAATAAGAGATATTTATGTATATATAATCTTAAGGCTAAGTATGCAATTTTGTCCCCTAATGTACCTTAGGCTGCCCTTCCTTCTTTTGATAATACAAGACTTGTCTCTTATAAAAATAAAAAACTTGTCCCCTAATGTATGTGCCATTTATCCATTTCTTCCTTAATGTTCTAGAAAAGTTTGAATATTATCCTTTTTATGTGGGGCATGTTCAACCAATTAACTTAAAGCGTAGGGTGATTCCAACTTAATTAATATAAATATATAATATATTTTTATGTTAAACCTAGATTTTAATGTTTAGTGAGATAGTAAGGAGTAAGGACTTGATTGAAAAACCTTATCTTATAAAGGATGAAATTGGAATTTTAAGCTAGGGATTAAATTGAAAAGCAACACAAACATTGGGACTTAGCCATAAAGGTATATATTTAGCTTAATCTTAAACGATATGTTATTTCTTAAAATCTGCCTTATAGACTCTTGAAATTAATTAAAATGCACCCTCCATCTTTGAGTGATCGATAATGTTTCCATCGTGGCAAAACATCCATGCATCTCTAAGATTTTTCATTTTTCGTTTCCACACGAGATTAAAAATTGAAATTGATAATATTTGAGCCCAACTGGTTCTTCACATTTGATCATATAACGCAGGTTTTGTAATGTATTAGGTGGTTGTCCTCCTCCAGTGATTAAGTTGGTTCCTGCAGTTTGTATTATCATGTTTGCTGTATGGGGTCTTGGACCATTTTTGCGTTATACTAGAAGCCTACTCCTCCATGTATGTTGACTCAACTTTTGTTTCTTATGTTGCATTGCACTTGCTCATTCTCCTCTTTAATTGTATTCTATTGTTTTAATATTGTTCGATAATTGACTTGCAGGGGAGCGATAATAACTGGAAAAAAAGTCGCACGTATAAAGTCATGACCTTGTATCTTCAACCATTATTGCTATGGGCTGGAGTTGCACTTATTTGCAGGTCCTTGAAATATAGTATTTTATATGTTGAGACATAGACTTATCTTCTCAGTCCCTCTTTTAAAAATAGTTCTTCTCTGACTAGTGTAGTATAATGGTCCTGGTCTTGCTTTTGCAGAGCTTTGGATCCAGTAGTTTTACCTACAAAGCCTAGCCAAGTTGTGAAGCAACGTATATTAAATTTTGTTAGGTCGTTGTCCACGGTGCTGGCCTTTGCGTATTGCTTATCAAGGTGAGAGTTTGAGACCTTGCTGTTTGAAATGTTTAATGAGTTTTGACCTATAGAGTGAAGAAATCTGCTATTGCCATGAACAGAAAATGACCTGAATCTAGCTAGCAATGATATGTTACCCTTACTCGAGGCTTTTTGCTTATGAAAAAGATTCAATTTGATTAACCCTTGCCTTTTTTTGGTCTATTTTTCTTCTTCTTTTCCCATTTTTTCTTCTGGTGTAGTATGATTGAACAAGCTCAGAAGTTCTTCTCAGAGACCACCGAATCCAGTGACGCAAGAAATGTATGTACAGTATGCCATCACAATTGTAATCTACGCTTAATATATATCATGTTATCAAGTTTCTGTTGGTTGATAAACATACTTATGTGAAGATCATCTTATAGTGTCAAACCAGCTGTCTTTTGTTGTAAGCTCTGTGTTGTATGGTATTTCACATTATATTTAAAGCAACACACCTTTAATTATCGTGTCAATGTTTCTTGGACGATGCAATTCAGCAAAAGCTATATTTTGTACATGGCTATATTCCTTACCATTGCCTTGAAGACAATTTGAGTGGCGTCCAGTATCAGAAAGAGATTGGTCAAACAGCTATGAATTCCTGAATTTACGAACTTAAAGTACACGCAATATATGGAGTTAATATCTTTCATTTCAATTTTGCTTTAAATATCATTTTAAATGGGTTTGTATCCTTCAAGATGTGATTCTCACAGGAATTCTTGTTTCATGATTTATTAAAAACCATGGAAGTGTGAATATAAGTTCTATGCCTATGCAGATGGGCTTTCAATTTGCTTGGAAGGCCGTATACTCTGCAGTATGGGTTGCTGCTGTCTCACTGTTCATGGAGCTCTTGGGCTTTTCTACCCAAAAATGGCTTACAGCTGGAGGTCTTGGGACTGTGTTGCTGACACTAGCTGGCCGTGAAGTACATTTTCGATTATTATGGGTTGTGTTCTCTGTTCCTTCCAATTTTTTTTACTAATTATAGATTTTATCTCACTGTACCTACTAGATATTCACAAACTTCCTTTCAAGTGTGATGATCCATGCAACTCGTCCTTTCATAGTCAATGAATGGATTCAAACAAAGATTGAGGGCTATGAAGTTTCTGGCACGGTTGAGGTTTGTATGTTTGTGTTGAACCATGCACCTTTAGAAGTTTTAATGTCGTTAGAAACATCTTATATGAATGCAATAGACATTCAATATCGTTTCTATCAACTGCAGCATGTAGGATGGTGGTCGCCAACAATTATTAGAGGTGAAGATCGTGAAGCAGTTCACATACCAAACCATCAGTTTACCATTAACGTGGTTAGAAATCTGAGTCAAAAAACTCACTGGCGTATTAAAACCCACCTAGCCATCAGCCATTTGGACGTCAATAAAATCAATGTGAGCGTTATTTTATTTATTTCTTTTCAGTTGGGTAAGGGAGCGTTATTTTATTTTGAATATTTATATGTATATATGTGTATATATAGACACATGTATGACAAATGTATGTTTATCTCTCTGCAGAATATTGTCGCTGACATGCGGAAAGTCTTGGCGAAGAACCCTCAAGTTGAACAACAGAGGTTGCATAGAAGAGTATTTTTGGAGAACGTGAATCCTGAAAACCAGGCACTTTTGGTCGGTATTAAACATTTTGACATAAATATGATTTTTTATTCCTGAAGTTTTCAATTTTGTCTCGAAGTTGAATAACTTAAATATACTCCTCAACTAAAATGCCAAAAGCACACTAAAATGTTGCAAATTTAGCATATTCGTTTGACAAATTTTGCCTACTTGAATAAGAGTTGAACAAAGTGTTGGTATTATGAAATTGGTCGCTGCATCCATGGGTGTGCTTGGCTTACATTGTGTGGTGGAATAACTATGATTGAAAGGATGGTTTTTTTTTTTTTGGTCTGGGCGGCTTGCAAGCATCTCGAAAGATATATATATATATATATATATAATAGGTAGGGCGATTTTAAAATTCAAATTCTCAAGTATTGAGACAATTTTAAATCTCGATTTATCCCAAAGTTATTATATTGGGTGATTTAATCATTCGACTTATTCTCTGTCAAGACAAACTGCATTGTTTTTAGATAACGTCATAAATCTTTGTTTGTTTACACCAAATTGCAAGGGGCTCTCTCATGTTTCCTAACTGATTTTTGTGCTTTTTTGTTTTGCTAGATTTTGATATCCTGTTTTGTCAAGACTTCACACTTTGAAGAATACCTTTGTGTGAAGGTAAAACAACCTTCTTGGTTTGCTTTATTACTCATTATAATTTTCTTTTTTGTTTACTTCTCATTCTTTCAACTTTCAATTAAAGAAGTTCCAATAATTTAATCCATTTTGTCTGAAGCTCGTCTGAGTACGTTTTGGATTTTGAACCTATTTTTTCATTTTTTTAAATTTTAAACTAACTTGCTAGTTGGTGAAATCAGGAAGCTATAATCTTGGATCTACTTAGAGTCATTAGACATCACCGGGCTCGCCTCGCCACACCAATCCGCACAATGCAGAAGATGCATAGTGATTCGGACTTGGAAAGTGTTCCATTTTCTGATTCAATATTTGGTCGTGGTGGTGTGAATTTGAATCGCCGCATGCTAATGATCGAACCCCCATACAAAGTCTATGGAGAAGATAGAAAACAAAGCCACAGTAGGACGTCTCGCACAACTGGAGAACAAAATGGTAAGCCCATTGCACGGTCTTCGGGTGACAGCAAGGCAGCCAAAGAGGCAACACCATCTGACAGAAAGACAGAAGTCAGGACTGGAGAAGAAAAAGACTCTGATACAAAAAAGCATCCCAAAGTTCCAACGTCGATGTCTGATGATAATTCAAGTAATGAATCAAAACATAAGTCATCATCAAGATCTGCATCGAGTACAAACGGTATCTCTGGTATGCCTAGTTCTGATGCCAAAACAACTAGATCAGACACTGATAATTCATTTGAGGATCCTAGTACGAAGCAATCTGAAAATGGCTCGGGAAGTACCAAGCAGAACTACAAGTCTAACCATCCAGCTGTTTCCTTGCCGGAAGATGTTAAGAAAACAGGGGGAAGTTCAGCTACTTCACAGCCAAGGATAGAAGGTGAACAGACGCCAGTTTTAAAGCCATCAACATTGAAGCCTGGTGTTGAAGAGAACTTAATTCTTGGTGTTGCTTTGGATGGCTCTAAGAGAACCCTTCCTATTGAGGAGGACATGCCTTCTTCCCCTGAAACCGTGAAAGATTTGGCTGCACGCTGGAACGGGAATGGAACGAATGGGGCAACAACTCCGGATAAGGATACGAAAGATCAGACTCCACCTCCAAGTGAATAGTGACTAGTGAGACTAGTTGATGCAGTGCATTCAGATTGCTCGGTTGATAAGCAACTTTTTCAACTGTAAAATGCGCAGCATGGCCCTGTGAAGCCGCGTTCTATATATGCCGCATCAAGTACAGTTCCATGACTGTAGGGCGGGGGAACGTACATTTGTGTCGTTTCAACTCTTGCAGTTGCAGCAGTGAGTCGTGAAACAAAATACTGATTGGTTTCGGGAGCTGGTGCTGTTGATGGCAACCCTTTGGCTTCGCTGGGTTGAAGATGACTCAAATACATCCATTGGTAACCATTTTCACTTTGAAGAGGTTTCCATAGCAATAGGAGAGCTTCCATTTTGTGCAGAAAACTTGTATTCTGAAATATAAATTTGTACCAGGAAAGGATTTGCTGGATTGGATCAATTCCTATTGTAGAAGAATATGAATTTTCTTCAAGAGGATACTGGAAATTTTGCATATAAATTATAATGAACGAAATTCTCAGAGTATGTAGTTATTTTACAGAATTCTGTGTTCATGTTGCAAATTGAAATACTATACCAATCAAATTTAG

mRNA sequence

GGGCCCTGTTTTCATTTCTTTTTCTTCCTACCTACGCATGAGCAGGCGAAATAGAGACGGTGGTTTCGATCGGAAGCTGTAGAAATTTGGCCGCCAACGTATTTTGCTTCGATTTGACCAGCTTTCAGCTGTCATCGTGCTGCATAGGAGTTTTATTGTTCAAGTGTCAACATCTCAGCATTTATTTAGTTTGAGAAATGGTTATTGTCGGTTCTTTGCAACTGTCCTATCATCTGGGACCCTGGAGGAACCGTTTGTATGAAGAAAACCTTAAGTTTGCCACTCAGAGCAATAACATACGGTTATTGAGTGCAGCCAGTCCATCGCCTCGTTTATTTCAGCAGAAGAACACTTGGAGCACTCATTTATTTAGCATGAAGTACTCACCTAATTACACTGTACCTTTTAGATACAATGTATTTAGGTGCCACTCGTCATTGATGACAGACCAACCACTGGATCCTCCTGGGATTAAGGCAGCTGTAGTGGCTTTGACAAGGTTTTGTAATGTATTAGGTGGTTGTCCTCCTCCAGTGATTAAGTTGGTTCCTGCAGTTTGTATTATCATGTTTGCTGTATGGGGTCTTGGACCATTTTTGCGTTATACTAGAAGCCTACTCCTCCATGGGAGCGATAATAACTGGAAAAAAAGTCGCACGTATAAAGTCATGACCTTGTATCTTCAACCATTATTGCTATGGGCTGGAGTTGCACTTATTTGCAGAGCTTTGGATCCAGTAGTTTTACCTACAAAGCCTAGCCAAGTTGTGAAGCAACGTATATTAAATTTTGTTAGGTCGTTGTCCACGGTGCTGGCCTTTGCGTATTGCTTATCAAGTATGATTGAACAAGCTCAGAAGTTCTTCTCAGAGACCACCGAATCCAGTGACGCAAGAAATATGGGCTTTCAATTTGCTTGGAAGGCCGTATACTCTGCAGTATGGGTTGCTGCTGTCTCACTGTTCATGGAGCTCTTGGGCTTTTCTACCCAAAAATGGCTTACAGCTGGAGGTCTTGGGACTGTGTTGCTGACACTAGCTGGCCGTGAAATATTCACAAACTTCCTTTCAAGTGTGATGATCCATGCAACTCGTCCTTTCATAGTCAATGAATGGATTCAAACAAAGATTGAGGGCTATGAAGTTTCTGGCACGGTTGAGGTTTGTATGTTTGTGTTGAACCATGCACCTTTAGAAGTTTTAATGTCGTTAGAAACATCTTATATGAATGCAATAGACATTCAATATCGTTTCTATCAACTGCAGCATGTAGGATGGTGGTCGCCAACAATTATTAGAGGTGAAGATCGTGAAGCAGTTCACATACCAAACCATCAGTTTACCATTAACGTGGTTAGAAATCTGAGTCAAAAAACTCACTGGCGTATTAAAACCCACCTAGCCATCAGCCATTTGGACGTCAATAAAATCAATAATATTGTCGCTGACATGCGGAAAGTCTTGGCGAAGAACCCTCAAGTTGAACAACAGAGGTTGCATAGAAGAGTATTTTTGGAGAACGTGAATCCTGAAAACCAGGCACTTTTGATTTTGATATCCTGTTTTGTCAAGACTTCACACTTTGAAGAATACCTTTGTGTGAAGGAAGCTATAATCTTGGATCTACTTAGAGTCATTAGACATCACCGGGCTCGCCTCGCCACACCAATCCGCACAATGCAGAAGATGCATAGTGATTCGGACTTGGAAAGTGTTCCATTTTCTGATTCAATATTTGGTCGTGGTGGTGTGAATTTGAATCGCCGCATGCTAATGATCGAACCCCCATACAAAGTCTATGGAGAAGATAGAAAACAAAGCCACAGTAGGACGTCTCGCACAACTGGAGAACAAAATGGTAAGCCCATTGCACGGTCTTCGGGTGACAGCAAGGCAGCCAAAGAGGCAACACCATCTGACAGAAAGACAGAAGTCAGGACTGGAGAAGAAAAAGACTCTGATACAAAAAAGCATCCCAAAGTTCCAACGTCGATGTCTGATGATAATTCAAGTAATGAATCAAAACATAAGTCATCATCAAGATCTGCATCGAGTACAAACGGTATCTCTGGTATGCCTAGTTCTGATGCCAAAACAACTAGATCAGACACTGATAATTCATTTGAGGATCCTAGTACGAAGCAATCTGAAAATGGCTCGGGAAGTACCAAGCAGAACTACAAGTCTAACCATCCAGCTGTTTCCTTGCCGGAAGATGTTAAGAAAACAGGGGGAAGTTCAGCTACTTCACAGCCAAGGATAGAAGGTGAACAGACGCCAGTTTTAAAGCCATCAACATTGAAGCCTGGTGTTGAAGAGAACTTAATTCTTGGTGTTGCTTTGGATGGCTCTAAGAGAACCCTTCCTATTGAGGAGGACATGCCTTCTTCCCCTGAAACCGTGAAAGATTTGGCTGCACGCTGGAACGGGAATGGAACGAATGGGGCAACAACTCCGGATAAGGATACGAAAGATCAGACTCCACCTCCAAGTGAATAGTGACTAGTGAGACTAGTTGATGCAGTGCATTCAGATTGCTCGGTTGATAAGCAACTTTTTCAACTGTAAAATGCGCAGCATGGCCCTGTGAAGCCGCGTTCTATATATGCCGCATCAAGTACAGTTCCATGACTGTAGGGCGGGGGAACGTACATTTGTGTCGTTTCAACTCTTGCAGTTGCAGCAGTGAGTCGTGAAACAAAATACTGATTGGTTTCGGGAGCTGGTGCTGTTGATGGCAACCCTTTGGCTTCGCTGGGTTGAAGATGACTCAAATACATCCATTGGTAACCATTTTCACTTTGAAGAGGTTTCCATAGCAATAGGAGAGCTTCCATTTTGTGCAGAAAACTTGTATTCTGAAATATAAATTTGTACCAGGAAAGGATTTGCTGGATTGGATCAATTCCTATTGTAGAAGAATATGAATTTTCTTCAAGAGGATACTGGAAATTTTGCATATAAATTATAATGAACGAAATTCTCAGAGTATGTAGTTATTTTACAGAATTCTGTGTTCATGTTGCAAATTGAAATACTATACCAATCAAATTTAG

Coding sequence (CDS)

ATGGTTATTGTCGGTTCTTTGCAACTGTCCTATCATCTGGGACCCTGGAGGAACCGTTTGTATGAAGAAAACCTTAAGTTTGCCACTCAGAGCAATAACATACGGTTATTGAGTGCAGCCAGTCCATCGCCTCGTTTATTTCAGCAGAAGAACACTTGGAGCACTCATTTATTTAGCATGAAGTACTCACCTAATTACACTGTACCTTTTAGATACAATGTATTTAGGTGCCACTCGTCATTGATGACAGACCAACCACTGGATCCTCCTGGGATTAAGGCAGCTGTAGTGGCTTTGACAAGGTTTTGTAATGTATTAGGTGGTTGTCCTCCTCCAGTGATTAAGTTGGTTCCTGCAGTTTGTATTATCATGTTTGCTGTATGGGGTCTTGGACCATTTTTGCGTTATACTAGAAGCCTACTCCTCCATGGGAGCGATAATAACTGGAAAAAAAGTCGCACGTATAAAGTCATGACCTTGTATCTTCAACCATTATTGCTATGGGCTGGAGTTGCACTTATTTGCAGAGCTTTGGATCCAGTAGTTTTACCTACAAAGCCTAGCCAAGTTGTGAAGCAACGTATATTAAATTTTGTTAGGTCGTTGTCCACGGTGCTGGCCTTTGCGTATTGCTTATCAAGTATGATTGAACAAGCTCAGAAGTTCTTCTCAGAGACCACCGAATCCAGTGACGCAAGAAATATGGGCTTTCAATTTGCTTGGAAGGCCGTATACTCTGCAGTATGGGTTGCTGCTGTCTCACTGTTCATGGAGCTCTTGGGCTTTTCTACCCAAAAATGGCTTACAGCTGGAGGTCTTGGGACTGTGTTGCTGACACTAGCTGGCCGTGAAATATTCACAAACTTCCTTTCAAGTGTGATGATCCATGCAACTCGTCCTTTCATAGTCAATGAATGGATTCAAACAAAGATTGAGGGCTATGAAGTTTCTGGCACGGTTGAGGTTTGTATGTTTGTGTTGAACCATGCACCTTTAGAAGTTTTAATGTCGTTAGAAACATCTTATATGAATGCAATAGACATTCAATATCGTTTCTATCAACTGCAGCATGTAGGATGGTGGTCGCCAACAATTATTAGAGGTGAAGATCGTGAAGCAGTTCACATACCAAACCATCAGTTTACCATTAACGTGGTTAGAAATCTGAGTCAAAAAACTCACTGGCGTATTAAAACCCACCTAGCCATCAGCCATTTGGACGTCAATAAAATCAATAATATTGTCGCTGACATGCGGAAAGTCTTGGCGAAGAACCCTCAAGTTGAACAACAGAGGTTGCATAGAAGAGTATTTTTGGAGAACGTGAATCCTGAAAACCAGGCACTTTTGATTTTGATATCCTGTTTTGTCAAGACTTCACACTTTGAAGAATACCTTTGTGTGAAGGAAGCTATAATCTTGGATCTACTTAGAGTCATTAGACATCACCGGGCTCGCCTCGCCACACCAATCCGCACAATGCAGAAGATGCATAGTGATTCGGACTTGGAAAGTGTTCCATTTTCTGATTCAATATTTGGTCGTGGTGGTGTGAATTTGAATCGCCGCATGCTAATGATCGAACCCCCATACAAAGTCTATGGAGAAGATAGAAAACAAAGCCACAGTAGGACGTCTCGCACAACTGGAGAACAAAATGGTAAGCCCATTGCACGGTCTTCGGGTGACAGCAAGGCAGCCAAAGAGGCAACACCATCTGACAGAAAGACAGAAGTCAGGACTGGAGAAGAAAAAGACTCTGATACAAAAAAGCATCCCAAAGTTCCAACGTCGATGTCTGATGATAATTCAAGTAATGAATCAAAACATAAGTCATCATCAAGATCTGCATCGAGTACAAACGGTATCTCTGGTATGCCTAGTTCTGATGCCAAAACAACTAGATCAGACACTGATAATTCATTTGAGGATCCTAGTACGAAGCAATCTGAAAATGGCTCGGGAAGTACCAAGCAGAACTACAAGTCTAACCATCCAGCTGTTTCCTTGCCGGAAGATGTTAAGAAAACAGGGGGAAGTTCAGCTACTTCACAGCCAAGGATAGAAGGTGAACAGACGCCAGTTTTAAAGCCATCAACATTGAAGCCTGGTGTTGAAGAGAACTTAATTCTTGGTGTTGCTTTGGATGGCTCTAAGAGAACCCTTCCTATTGAGGAGGACATGCCTTCTTCCCCTGAAACCGTGAAAGATTTGGCTGCACGCTGGAACGGGAATGGAACGAATGGGGCAACAACTCCGGATAAGGATACGAAAGATCAGACTCCACCTCCAAGTGAATAG

Protein sequence

MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALDGSKRTLPIEEDMPSSPETVKDLAARWNGNGTNGATTPDKDTKDQTPPPSE
Homology
BLAST of MC10g0502 vs. ExPASy Swiss-Prot
Match: Q56X46 (Mechanosensitive ion channel protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MSL2 PE=2 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 2.2e-193
Identity = 400/766 (52.22%), Postives = 490/766 (63.97%), Query Frame = 0

Query: 1   MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSM 60
           M + G+LQLS+ LG  RN+ +  N + +     + + +          Q    +  L + 
Sbjct: 1   MALYGTLQLSHSLGLCRNQRF-CNPENSAMRRRLHISNGPLSLGVPLGQHGFSNILLSNY 60

Query: 61  KYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAV 120
              P  +VP R   FRCHS   + + ++ P +KA  V LT+   ++    P V KLVPAV
Sbjct: 61  LRRPICSVPCRTTAFRCHSFSASGKAIE-PAVKAVTVVLTKSHGLMQQF-PFVYKLVPAV 120

Query: 121 CIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDP 180
            +++F++WGL PF R  R++LL+ +DN WKKS TY VMT Y+QPLLLW G   ICRALDP
Sbjct: 121 ALLVFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDP 180

Query: 181 VVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFA 240
           VVLPT+ S++VK R+LNFVRSLSTVLAFAYCLSS+I+Q QK FSET+  SD RNMGFQFA
Sbjct: 181 VVLPTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFA 240

Query: 241 WKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300
            KA+YSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRP
Sbjct: 241 GKALYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRP 300

Query: 301 FIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGW 360
           F++NEWIQTKIEGYEVSGTVE                                   HVGW
Sbjct: 301 FVLNEWIQTKIEGYEVSGTVE-----------------------------------HVGW 360

Query: 361 WSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK 420
           WSPTIIRGEDREA+HIPNH+FT+NVVRNL+QKTHWRIKTHLAISHLDVNKINNIVADMRK
Sbjct: 361 WSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIKTHLAISHLDVNKINNIVADMRK 420

Query: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI 480
           VLAKNP VEQQRLHRRVFLENV PENQAL ILISCFVKTSH EEYL VKEAI+LDLLRVI
Sbjct: 421 VLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVKTSHHEEYLGVKEAILLDLLRVI 480

Query: 481 RHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQ 540
            HHRARLATPIRT++KM++++D+E+ PF +S++  GGV   R +++IEP YK+ GED+ +
Sbjct: 481 SHHRARLATPIRTIRKMYTETDVENTPFGESMY--GGVTSRRPLMLIEPAYKINGEDKSK 540

Query: 541 SHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMS 600
           S +R ++ T EQ  K      G +  +KE +  D K  V+ GE   SDT K P+      
Sbjct: 541 SQNRAAKPTAEQENK------GSNPKSKETSSPDLKANVKVGESPVSDTNKVPE------ 600

Query: 601 DDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQN 660
                                          +T       +   P T +    SG+ K  
Sbjct: 601 -------------------------------ETVAKPVIKAVSKPPTPKDTETSGTEKPK 660

Query: 661 YKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALDGSKR 720
                         K++GG+  +++       T     STL    EEN++LGVAL+GSKR
Sbjct: 661 -------------AKRSGGTIKSTKTDETDSSTSSASRSTL----EENIVLGVALEGSKR 666

Query: 721 TLPIEEDMPSSP-ETVKDLAARWNGNGTNGATTPDKDTKDQTPPPS 766
           TLPIEE++ S P ET          +G NG    DK+ KD    P+
Sbjct: 721 TLPIEEEIHSPPMETDAKELTGARRSGGNGPLVADKEQKDSQSQPN 666

BLAST of MC10g0502 vs. ExPASy Swiss-Prot
Match: Q8L7W1 (Mechanosensitive ion channel protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MSL3 PE=1 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 2.5e-168
Identity = 352/691 (50.94%), Postives = 443/691 (64.11%), Query Frame = 0

Query: 49  QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 108
           +++ WS  L          V  R N F C S+L      + P +K+  V  TR  + LGG
Sbjct: 48  RQDVWSLQLLESLSGSIVPVSSRCNAFVCRSALSPGNGNEGPILKSTAVIFTRVYDALGG 107

Query: 109 CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLH-GSDNNWKKSRTYKVMTLYLQPLLL 168
             P ++KL+PAV I+ FA WGL P LR  R+ L   G+D N +KS T  ++  YLQPLLL
Sbjct: 108 -NPHLVKLIPAVGILAFATWGLRPLLRLARTTLFEKGNDANSQKSSTQYIVVSYLQPLLL 167

Query: 169 WAGVALICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETT 228
           W+G  L+CR LDP+VLP+   Q +KQR+L F RS+STVLAF+ CLSS+++Q QKFF ET 
Sbjct: 168 WSGAILLCRTLDPIVLPSSAGQAIKQRLLIFARSISTVLAFSCCLSSLLQQVQKFFMETN 227

Query: 229 ESSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFT 288
             +D RNMGF FA KAVY+A WVAA SLFMELLGFSTQKWLTAGGLGTVLLTLAGREI T
Sbjct: 228 NPADTRNMGFSFAGKAVYTAAWVAAASLFMELLGFSTQKWLTAGGLGTVLLTLAGREILT 287

Query: 289 NFLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAID 348
           NFLSS+MIHATRPF++NEWIQTKI GYEVSGTVE                          
Sbjct: 288 NFLSSIMIHATRPFVLNEWIQTKIGGYEVSGTVE-------------------------- 347

Query: 349 IQYRFYQLQHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLD 408
                     VGWWSPTIIRG+DREAVHIPNHQF++N+VRNL+QKTHWRIKTHLAISHLD
Sbjct: 348 ---------QVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWRIKTHLAISHLD 407

Query: 409 VNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLC 468
           V+KINNIVADMRKVL+KNPQ+EQQ++HRRVFLE+++PENQAL ILISCFVKTS FEEYLC
Sbjct: 408 VSKINNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALRILISCFVKTSRFEEYLC 467

Query: 469 VKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMI 528
           VKEA++LDLL VIRHH ARLATPIRT+Q+M +++++++  FSD +F +    +NRR ++I
Sbjct: 468 VKEAVLLDLLTVIRHHGARLATPIRTVQRMRNEAEVDTAGFSDIVFNQAA--MNRRYMLI 527

Query: 529 EPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEV-RTGEEKD 588
           EP YK+  +D  +S    S + G+++  P  +S    +   +  PS+ K E    G    
Sbjct: 528 EPSYKINSDDNSKS---PSPSPGQKSPSPGQKS---EERDLQEEPSETKAETENNGSVPV 587

Query: 589 SDTKKHPKVPTSMSDDNSSNESKHKSSS------RSASSTNGISGMPSSDAKTTRSDTDN 648
           S+ KK  +     S+ N+  +    S+S      +S        G P    K   SD + 
Sbjct: 588 SNAKKENQKAALGSNSNTGTKGSSTSTSDQPVAQKSEEKKKESVGDPHKAEKDEVSDDEA 647

Query: 649 SFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKPST 708
           + E   T +S+   GS K N +S            + GG S TS                
Sbjct: 648 TIE--QTLKSKAKQGSEKNNGESK----------ARDGGGSGTSSL-------------- 664

Query: 709 LKPGVEENLILGVALDGSKRTLPIEEDMPSS 732
               +EENL+LGVALDGSKRTLPI+E+  +S
Sbjct: 708 ----LEENLVLGVALDGSKRTLPIDEEHKAS 664

BLAST of MC10g0502 vs. ExPASy Swiss-Prot
Match: P0AEB6 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=83334 GN=ynaI PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 5.2e-17
Identity = 66/246 (26.83%), Postives = 116/246 (47.15%), Query Frame = 0

Query: 250 VAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIVNEWIQT 309
           V  V L+ E  G S    LT GG+G + + +AG++I +NF S +M++  RPF + +WI++
Sbjct: 128 VVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRS 187

Query: 310 KIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGWWSPTIIRGE 369
                E  GTV                                     +G W  T I   
Sbjct: 188 PDRNIE--GTV-----------------------------------AEIG-WRITKITTF 247

Query: 370 DREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVE 429
           D   +++PN  F+   V N  + T+ RI T + + + D  K+  IV  +R++L  +P ++
Sbjct: 248 DNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAID 307

Query: 430 QQRLHRRVFLENVNP-ENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLA 489
           Q    R+  L   N   + +L I++ CF KT+ + E+L  ++ + L ++ +++ H A  A
Sbjct: 308 Q----RQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFA 331

Query: 490 TPIRTM 495
            P +T+
Sbjct: 368 FPSQTL 331

BLAST of MC10g0502 vs. ExPASy Swiss-Prot
Match: P0AEB5 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) OX=83333 GN=ynaI PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 5.2e-17
Identity = 66/246 (26.83%), Postives = 116/246 (47.15%), Query Frame = 0

Query: 250 VAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIVNEWIQT 309
           V  V L+ E  G S    LT GG+G + + +AG++I +NF S +M++  RPF + +WI++
Sbjct: 128 VVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIMLYFDRPFSIGDWIRS 187

Query: 310 KIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGWWSPTIIRGE 369
                E  GTV                                     +G W  T I   
Sbjct: 188 PDRNIE--GTV-----------------------------------AEIG-WRITKITTF 247

Query: 370 DREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVE 429
           D   +++PN  F+   V N  + T+ RI T + + + D  K+  IV  +R++L  +P ++
Sbjct: 248 DNRPLYVPNSLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAID 307

Query: 430 QQRLHRRVFLENVNP-ENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLA 489
           Q    R+  L   N   + +L I++ CF KT+ + E+L  ++ + L ++ +++ H A  A
Sbjct: 308 Q----RQTLLVYFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFA 331

Query: 490 TPIRTM 495
            P +T+
Sbjct: 368 FPSQTL 331

BLAST of MC10g0502 vs. ExPASy Swiss-Prot
Match: Q58543 (Large-conductance mechanosensitive channel MscMJLR OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ1143 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 4.4e-08
Identity = 77/356 (21.63%), Postives = 147/356 (41.29%), Query Frame = 0

Query: 116 LVPAVCIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALIC 175
           ++  + II+F V G     +Y  +L+   +D   KKS   ++  L ++ L L   +A+I 
Sbjct: 20  ILSLISIILFIVIG-----KYANALIERLADKLHKKS-GIELDELLIRALSLPVAIAIIL 79

Query: 176 RAL-----DPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESS 235
                      +LP+  + V +  +  F+  L  V+ F   L+ ++E+            
Sbjct: 80  SGFYFGVNFLYLLPSLKTAVNEGILTAFI--LCVVVFFDRFLNELVERYLALTISKKTKK 139

Query: 236 DARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFL 295
           D  +       K V   VWV  + L +  LG+  +  L   G+G + + LA + + +N +
Sbjct: 140 DVDDQIVVLTKKLVRLVVWVVGLLLILSNLGYDIKTLLAGLGIGGLAVALASQNLVSNLI 199

Query: 296 SSVMIHATRPFIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQY 355
           + ++I   +PF +  WI T   G   SG VE                         DI  
Sbjct: 200 AGLIILTDKPFKIGNWI-TFSGG---SGIVE-------------------------DIGI 259

Query: 356 RFYQLQHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISH-LDVN 415
           R            T IR  D   + +PN +    +++N+  K  W++ T + +++   V 
Sbjct: 260 R-----------STKIRATDNSIIVVPNSKLIDEIIQNVPSKNKWKVSTTIGVTYNTPVE 319

Query: 416 KINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEY 466
           KI      ++ +L ++P VE + +   V+ +     + +L I +  ++K S +  Y
Sbjct: 320 KIRKAEEIIKNILLEHPNVEDEPI--TVYFKEFG--DWSLNIQVVYYIKNSRYNGY 323

BLAST of MC10g0502 vs. NCBI nr
Match: XP_022136725.1 (mechanosensitive ion channel protein 2, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1410 bits (3651), Expect = 0.0
Identity = 731/766 (95.43%), Postives = 731/766 (95.43%), Query Frame = 0

Query: 1   MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSM 60
           MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSM
Sbjct: 1   MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSM 60

Query: 61  KYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAV 120
           KYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAV
Sbjct: 61  KYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAV 120

Query: 121 CIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDP 180
           CIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDP
Sbjct: 121 CIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDP 180

Query: 181 VVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFA 240
           VVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFA
Sbjct: 181 VVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFA 240

Query: 241 WKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300
           WKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP
Sbjct: 241 WKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300

Query: 301 FIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGW 360
           FIVNEWIQTKIEGYEVSGTVE                                   HVGW
Sbjct: 301 FIVNEWIQTKIEGYEVSGTVE-----------------------------------HVGW 360

Query: 361 WSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK 420
           WSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK
Sbjct: 361 WSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK 420

Query: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI 480
           VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI
Sbjct: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI 480

Query: 481 RHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQ 540
           RHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQ
Sbjct: 481 RHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQ 540

Query: 541 SHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMS 600
           SHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMS
Sbjct: 541 SHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMS 600

Query: 601 DDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQN 660
           DDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQN
Sbjct: 601 DDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQN 660

Query: 661 YKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALDGSKR 720
           YKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALDGSKR
Sbjct: 661 YKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALDGSKR 720

Query: 721 TLPIEEDMPSSPETVKDLAARWNGNGTNGATTPDKDTKDQTPPPSE 766
           TLPIEEDMPSSPETVKDLAARWNGNGTNGATTPDKDTKDQTPPPSE
Sbjct: 721 TLPIEEDMPSSPETVKDLAARWNGNGTNGATTPDKDTKDQTPPPSE 731

BLAST of MC10g0502 vs. NCBI nr
Match: KAG6577342.1 (Mechanosensitive ion channel protein 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1167 bits (3018), Expect = 0.0
Identity = 618/770 (80.26%), Postives = 667/770 (86.62%), Query Frame = 0

Query: 1   MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSM 60
           MV+VGSLQLSYHLGPW+N L EENLKF + SNNIRL + ASPS  LFQQKNTWS+HLFS+
Sbjct: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60

Query: 61  KYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAV 120
           KY P+Y VP RYN FRCHSSLMT+QPLDP G+KAA+VALTRFCNVLGGCPPPV+K+VPAV
Sbjct: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120

Query: 121 CIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDP 180
            I++FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW G  LICRALDP
Sbjct: 121 SIMIFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDP 180

Query: 181 VVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFA 240
           VVLPT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+ESSDARNMGFQFA
Sbjct: 181 VVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFA 240

Query: 241 WKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300
           WKAVYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP
Sbjct: 241 WKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300

Query: 301 FIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGW 360
           F+VNEWIQT+IEGYEVSGTVE                                   HVGW
Sbjct: 301 FVVNEWIQTRIEGYEVSGTVE-----------------------------------HVGW 360

Query: 361 WSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK 420
           WSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIKTHLAISHLDV+KINNIVADMRK
Sbjct: 361 WSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRK 420

Query: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI 480
           VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAI LDLLRVI
Sbjct: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVI 480

Query: 481 RHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQ 540
           RHHRARLATPIRT+QKMHSDSDLESVPFSDSIFG GG  LNRRMLMIEPPYKVYG+DRKQ
Sbjct: 481 RHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDRKQ 540

Query: 541 SHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMS 600
           SHSRTSRTTGEQNGKPIARSSGD+KAAKE TPS+RKTEV+TGE +DSD K H KVP S S
Sbjct: 541 SHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTEVKTGEGRDSDAKIHSKVPMSTS 600

Query: 601 DDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDP-STKQSENGSGST-K 660
           +D  S+ESKHKSSSRS SSTNGI+ MP+SDAKTT SD DNS +D  S+K+S+N SGS  K
Sbjct: 601 EDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGSNNK 660

Query: 661 QNYKSNHPAVSLPEDVKKTGGS-SATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALDG 720
           QNYK  H +VS  EDVKK  G+ S+ SQPRIEG+QT    PST KPGVEEN+ILGVALDG
Sbjct: 661 QNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT--NPSTTKPGVEENIILGVALDG 720

Query: 721 SKRTLPIEEDMPSSPETV---KDLAAR-WNGNGTNGATTPDKDTKDQTPP 763
           SKRTLPIE+D PSSP+T    KDLAA   NGNG NGATTPDK+TK Q+PP
Sbjct: 721 SKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGANGATTPDKETKRQSPP 731

BLAST of MC10g0502 vs. NCBI nr
Match: XP_023552794.1 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552796.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552797.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552798.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552799.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023552800.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1162 bits (3005), Expect = 0.0
Identity = 614/771 (79.64%), Postives = 668/771 (86.64%), Query Frame = 0

Query: 1   MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSM 60
           MV+VGSLQLSYHLGPW+N L EENLKF + SNNIRLL+ ASPS  LFQQKNTWS+HLFS+
Sbjct: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLLNVASPSSLLFQQKNTWSSHLFSL 60

Query: 61  KYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAV 120
           KY P+Y VP RYN FRCHSSLMT+QPLDPPG+KAA+VALTRFCNVLGGCPPPV+K+VPAV
Sbjct: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPPGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120

Query: 121 CIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDP 180
            I++F+VWG+GPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW G  LICRALDP
Sbjct: 121 SIMIFSVWGIGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDP 180

Query: 181 VVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFA 240
           VVLPT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+ESSDARNMGFQFA
Sbjct: 181 VVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFA 240

Query: 241 WKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300
           WKAVYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP
Sbjct: 241 WKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300

Query: 301 FIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGW 360
           F+VNEWIQT+IEGYEVSGTVE                                   HVGW
Sbjct: 301 FVVNEWIQTRIEGYEVSGTVE-----------------------------------HVGW 360

Query: 361 WSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK 420
           WSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIKTHLAISHLDV+KINNIVADMRK
Sbjct: 361 WSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRK 420

Query: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI 480
           VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAI LDLLRVI
Sbjct: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVI 480

Query: 481 RHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQ 540
           RHHRARLATPIRT+QKMHSDSDLES+PFSDSIFG GG  LNRRMLMIEPPYK+YG+DRKQ
Sbjct: 481 RHHRARLATPIRTVQKMHSDSDLESIPFSDSIFGHGGATLNRRMLMIEPPYKIYGDDRKQ 540

Query: 541 SHSRTSRTT-GEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSM 600
           SH RTSRTT GEQN KPIARSSGD+KA+KE TPS+RKTEV+TGE +DSDTK H KVP S 
Sbjct: 541 SHGRTSRTTTGEQNSKPIARSSGDNKASKETTPSERKTEVKTGEGRDSDTKIHSKVPMST 600

Query: 601 SDDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDP-STKQSENGSGST- 660
           S+D  S+ESKHKSSSRS SSTNGI+ MPSSDAKTT SDTDNS +D  S+K+S+N SGS  
Sbjct: 601 SEDKPSSESKHKSSSRSVSSTNGIADMPSSDAKTTTSDTDNSMQDSGSSKKSKNSSGSNN 660

Query: 661 KQNYKSNHPAVSLPEDVKKTGGS-SATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALD 720
           KQNYK  H +VS  EDVKK  G+ S+ SQPRIEG+QT    PST KPGVEEN+ILGVALD
Sbjct: 661 KQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT--NPSTTKPGVEENIILGVALD 720

Query: 721 GSKRTLPIEEDMPSSPETV---KDLAAR-WNGNGTNGATTPDKDTKDQTPP 763
           GSKRTLPI++D PSSP+T    KDLAA   NGNG  GATTPDK+TK Q+PP
Sbjct: 721 GSKRTLPIDDDTPSSPQTTLGAKDLAAASLNGNGATGATTPDKETKRQSPP 732

BLAST of MC10g0502 vs. NCBI nr
Match: XP_022929450.1 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929451.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929452.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929453.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929454.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022929455.1 mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1159 bits (2999), Expect = 0.0
Identity = 615/770 (79.87%), Postives = 664/770 (86.23%), Query Frame = 0

Query: 1   MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSM 60
           MV+VGSLQLSYHLGPW+N L EENLKF + SNNIRL + ASPS  LFQQKNTWS+HLFS+
Sbjct: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60

Query: 61  KYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAV 120
           KY P+Y VP RYN FRCHSSLMT+QPLDP G+KAA+VALTRFCNVLGGCPPPV+K+VPAV
Sbjct: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120

Query: 121 CIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDP 180
            I++FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW G  LICRALDP
Sbjct: 121 SIMIFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDP 180

Query: 181 VVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFA 240
           VVLPT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+ESSDARNMGFQFA
Sbjct: 181 VVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFA 240

Query: 241 WKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300
           WKAVYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP
Sbjct: 241 WKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300

Query: 301 FIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGW 360
           F+VNEWIQT+IEGYEVSGTVE                                   HVGW
Sbjct: 301 FVVNEWIQTRIEGYEVSGTVE-----------------------------------HVGW 360

Query: 361 WSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK 420
           WSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIKTHLAISHLDV+KINNIVADMRK
Sbjct: 361 WSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRK 420

Query: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI 480
           VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAI LDLLRVI
Sbjct: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVI 480

Query: 481 RHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQ 540
           RHHRARLATPIRT+QKMHSDSDLESVPFSDSIFG GG  LNRRMLMIEPPYKVYG+DRKQ
Sbjct: 481 RHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDRKQ 540

Query: 541 SHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMS 600
           SHSRTSRTTGEQNGKPIARSSGD+KAAKE TPS+RKTEV+ GE +DSD K H KVP S S
Sbjct: 541 SHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTEVKPGEGRDSDAKIHSKVPMSTS 600

Query: 601 DDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDP-STKQSENGSGST-K 660
           +D  S+ESKHKSSSRS SSTNGI+ MP+SDAKTT SD DNS +D  S+K+S+N SGS  K
Sbjct: 601 EDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGSNNK 660

Query: 661 QNYKSNHPAVSLPEDVKKTGGS-SATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALDG 720
           QNYK  H +VS  EDVKK  G+ S+ SQPRIEG+QT    PST KPGVEEN+ILGVALDG
Sbjct: 661 QNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT--NPSTTKPGVEENIILGVALDG 720

Query: 721 SKRTLPIEEDMPSSPETV---KDLAAR-WNGNGTNGATTPDKDTKDQTPP 763
           SKRTLPIE+D PSSP+T    KDLAA   NGNG  GATTPDK+TK  +PP
Sbjct: 721 SKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATTPDKETKRLSPP 731

BLAST of MC10g0502 vs. NCBI nr
Match: KAG7015430.1 (Mechanosensitive ion channel protein 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1159 bits (2999), Expect = 0.0
Identity = 621/774 (80.23%), Postives = 672/774 (86.82%), Query Frame = 0

Query: 1   MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSM 60
           MV+VGSLQLSYHLGPW+N L EENLKF + SNNIRL + ASPS  LFQQKNTWS+HLFS+
Sbjct: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60

Query: 61  KYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAV 120
           KY P+Y VP RYN FRCHSSLMT+QPLDP G+KAA+VALTRFCNVLGGCPPPV+K+VPAV
Sbjct: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120

Query: 121 CIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDP 180
            I++FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPL LW G  LICRALDP
Sbjct: 121 SIMIFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLFLWTGATLICRALDP 180

Query: 181 VVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFA 240
           VVLPT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+ESSDARNMGFQFA
Sbjct: 181 VVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFA 240

Query: 241 WKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300
           WKAVYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP
Sbjct: 241 WKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300

Query: 301 FIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGW 360
           F+VNEWIQT+IEGYEVSGTVE       H   E            +   + F QLQHVGW
Sbjct: 301 FVVNEWIQTRIEGYEVSGTVE------KHVLYE-----------HVRHYFHFNQLQHVGW 360

Query: 361 WSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK 420
           WSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIKTHLAISHLDV+KINNIVADMRK
Sbjct: 361 WSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRK 420

Query: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI 480
           VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAI LDLLRVI
Sbjct: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVI 480

Query: 481 RHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQ 540
           RHHRARLATPIRT+QKMHSDSDLESVPFSDSIFG GG  LNRRMLMIEPPYK+YG+DRKQ
Sbjct: 481 RHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGATLNRRMLMIEPPYKIYGDDRKQ 540

Query: 541 SHSRTSRTT-GEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSM 600
           SHSRTSRTT GEQN KPIARSSGD+KAAKE TPS+RKTEV+TGE +DSDTK H KVP S 
Sbjct: 541 SHSRTSRTTTGEQNSKPIARSSGDNKAAKETTPSERKTEVKTGEGRDSDTKIHSKVPMST 600

Query: 601 SDDNSSNESKHKSSSRSASST--NGISGMPSSDAKTTRSDTDNSFEDP-STKQSENGSGS 660
           S+D  S+ESKHKSSSRS SST  NGI+ MP+SDAKTT SD DNS +D  S+K+S+N SGS
Sbjct: 601 SEDKPSSESKHKSSSRSVSSTSTNGIADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGS 660

Query: 661 T-KQNYKSNHPAVSLPEDVKKTGGS-SATSQPRIEGEQTPVLKPSTLKPG-VEENLILGV 720
             KQNYK  H +VS  EDVKK  G+ S  SQPRIEG+QT    PST K G VEEN+ILGV
Sbjct: 661 NNKQNYKPIHSSVSFQEDVKKPEGTTSPASQPRIEGDQTT--NPSTTKTGGVEENIILGV 720

Query: 721 ALDGSKRTLPIEEDMPSSPETV---KDLAAR-WNGNGTNGATTPDKDTKDQTPP 763
           ALDGSKRTLPIE+D PSSP+T    KDLAA   NGNG NGATTPDK+TK Q+PP
Sbjct: 721 ALDGSKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGANGATTPDKETKRQSPP 753

BLAST of MC10g0502 vs. ExPASy TrEMBL
Match: A0A6J1C4S5 (mechanosensitive ion channel protein 2, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111008365 PE=4 SV=1)

HSP 1 Score: 1410 bits (3651), Expect = 0.0
Identity = 731/766 (95.43%), Postives = 731/766 (95.43%), Query Frame = 0

Query: 1   MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSM 60
           MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSM
Sbjct: 1   MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSM 60

Query: 61  KYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAV 120
           KYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAV
Sbjct: 61  KYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAV 120

Query: 121 CIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDP 180
           CIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDP
Sbjct: 121 CIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDP 180

Query: 181 VVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFA 240
           VVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFA
Sbjct: 181 VVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFA 240

Query: 241 WKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300
           WKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP
Sbjct: 241 WKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300

Query: 301 FIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGW 360
           FIVNEWIQTKIEGYEVSGTVE                                   HVGW
Sbjct: 301 FIVNEWIQTKIEGYEVSGTVE-----------------------------------HVGW 360

Query: 361 WSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK 420
           WSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK
Sbjct: 361 WSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK 420

Query: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI 480
           VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI
Sbjct: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI 480

Query: 481 RHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQ 540
           RHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQ
Sbjct: 481 RHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQ 540

Query: 541 SHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMS 600
           SHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMS
Sbjct: 541 SHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMS 600

Query: 601 DDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQN 660
           DDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQN
Sbjct: 601 DDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQN 660

Query: 661 YKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALDGSKR 720
           YKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALDGSKR
Sbjct: 661 YKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALDGSKR 720

Query: 721 TLPIEEDMPSSPETVKDLAARWNGNGTNGATTPDKDTKDQTPPPSE 766
           TLPIEEDMPSSPETVKDLAARWNGNGTNGATTPDKDTKDQTPPPSE
Sbjct: 721 TLPIEEDMPSSPETVKDLAARWNGNGTNGATTPDKDTKDQTPPPSE 731

BLAST of MC10g0502 vs. ExPASy TrEMBL
Match: A0A6J1ES61 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436025 PE=4 SV=1)

HSP 1 Score: 1159 bits (2999), Expect = 0.0
Identity = 615/770 (79.87%), Postives = 664/770 (86.23%), Query Frame = 0

Query: 1   MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSM 60
           MV+VGSLQLSYHLGPW+N L EENLKF + SNNIRL + ASPS  LFQQKNTWS+HLFS+
Sbjct: 1   MVLVGSLQLSYHLGPWKNHLLEENLKFTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60

Query: 61  KYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAV 120
           KY P+Y VP RYN FRCHSSLMT+QPLDP G+KAA+VALTRFCNVLGGCPPPV+K+VPAV
Sbjct: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120

Query: 121 CIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDP 180
            I++FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW G  LICRALDP
Sbjct: 121 SIMIFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDP 180

Query: 181 VVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFA 240
           VVLPT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+ESSDARNMGFQFA
Sbjct: 181 VVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFA 240

Query: 241 WKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300
           WKAVYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP
Sbjct: 241 WKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300

Query: 301 FIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGW 360
           F+VNEWIQT+IEGYEVSGTVE                                   HVGW
Sbjct: 301 FVVNEWIQTRIEGYEVSGTVE-----------------------------------HVGW 360

Query: 361 WSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK 420
           WSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIKTHLAISHLDV+KINNIVADMRK
Sbjct: 361 WSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRK 420

Query: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI 480
           VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAI LDLLRVI
Sbjct: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVI 480

Query: 481 RHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQ 540
           RHHRARLATPIRT+QKMHSDSDLESVPFSDSIFG GG  LNRRMLMIEPPYKVYG+DRKQ
Sbjct: 481 RHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDRKQ 540

Query: 541 SHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMS 600
           SHSRTSRTTGEQNGKPIARSSGD+KAAKE TPS+RKTEV+ GE +DSD K H KVP S S
Sbjct: 541 SHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTEVKPGEGRDSDAKIHSKVPMSTS 600

Query: 601 DDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDP-STKQSENGSGST-K 660
           +D  S+ESKHKSSSRS SSTNGI+ MP+SDAKTT SD DNS +D  S+K+S+N SGS  K
Sbjct: 601 EDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGSNNK 660

Query: 661 QNYKSNHPAVSLPEDVKKTGGS-SATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALDG 720
           QNYK  H +VS  EDVKK  G+ S+ SQPRIEG+QT    PST KPGVEEN+ILGVALDG
Sbjct: 661 QNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT--NPSTTKPGVEENIILGVALDG 720

Query: 721 SKRTLPIEEDMPSSPETV---KDLAAR-WNGNGTNGATTPDKDTKDQTPP 763
           SKRTLPIE+D PSSP+T    KDLAA   NGNG  GATTPDK+TK  +PP
Sbjct: 721 SKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATTPDKETKRLSPP 731

BLAST of MC10g0502 vs. ExPASy TrEMBL
Match: A0A6J1JBP8 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483011 PE=4 SV=1)

HSP 1 Score: 1140 bits (2949), Expect = 0.0
Identity = 609/771 (78.99%), Postives = 661/771 (85.73%), Query Frame = 0

Query: 1   MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSM 60
           MV+VGSLQLSYHLGPW+N L EENLK  + SNNIRL + ASPS  LFQQKNTWS+HLFS+
Sbjct: 1   MVLVGSLQLSYHLGPWKNHLLEENLKLTSHSNNIRLQNVASPSSLLFQQKNTWSSHLFSL 60

Query: 61  KYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAV 120
           KY P+Y VP RYN FRCHSSLMT+QPLDP G+KAA+VALTRFCNVLGGCPPPV+K+VPAV
Sbjct: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120

Query: 121 CIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDP 180
            I++FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVM+LYLQPLLLW G  LICRALDP
Sbjct: 121 SIMIFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMSLYLQPLLLWTGATLICRALDP 180

Query: 181 VVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFA 240
           VVLPT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+ESSDARNMGFQFA
Sbjct: 181 VVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFA 240

Query: 241 WKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300
           WKAVYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP
Sbjct: 241 WKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300

Query: 301 FIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGW 360
           F+VNEWIQTKIEGYEVSGTVE                                   HVGW
Sbjct: 301 FVVNEWIQTKIEGYEVSGTVE-----------------------------------HVGW 360

Query: 361 WSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK 420
           WSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIKTHLAISHLDV+KINNIVADMRK
Sbjct: 361 WSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRK 420

Query: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI 480
           VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAI LDLLRVI
Sbjct: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVI 480

Query: 481 RHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQ 540
           RHHRARLATPIRT+QKMHSDSDLESVPFSDSIFG GG  LNRRMLMIEPPYKVYG+DRKQ
Sbjct: 481 RHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDRKQ 540

Query: 541 SHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMS 600
           SHSRTSRTTGEQN KPIARSSGD+KAAKE +PS+RK EV+TGE +DSDTK + KVP S S
Sbjct: 541 SHSRTSRTTGEQNSKPIARSSGDNKAAKETSPSERKMEVKTGEGRDSDTKIYSKVPMSTS 600

Query: 601 DDNS-SNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDP-STKQSENGSGST- 660
           +DN  S+ESKHKSSSRS SSTNGI+ MP+SDAKTT  D DNS +D  S+K+S+N SGS  
Sbjct: 601 EDNKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTTLDADNSMQDSGSSKKSKNSSGSNN 660

Query: 661 KQNYKSNHPAVSLPEDVKKTGGS-SATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALD 720
           KQNYK  H +VS  EDVKK  G+ S+ SQPRIEG+QT    P T KP VEEN+ILGVALD
Sbjct: 661 KQNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT--NPPTTKPSVEENIILGVALD 720

Query: 721 GSKRTLPIEEDMPSSPETV---KDLAAR-WNGNGTNGATTPDKDTKDQTPP 763
            SKRTLPIE+D PSSP+T    KDLAA   NGN  +GATTPDK+TK Q+PP
Sbjct: 721 RSKRTLPIEDDTPSSPQTSLGSKDLAAASLNGNVASGATTPDKETKRQSPP 732

BLAST of MC10g0502 vs. ExPASy TrEMBL
Match: A0A6J1EN69 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436025 PE=4 SV=1)

HSP 1 Score: 1126 bits (2912), Expect = 0.0
Identity = 603/770 (78.31%), Postives = 650/770 (84.42%), Query Frame = 0

Query: 1   MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSM 60
           MV+VGSLQLSYHLGPW+N L EENLKF                    QQKNTWS+HLFS+
Sbjct: 1   MVLVGSLQLSYHLGPWKNHLLEENLKF--------------------QQKNTWSSHLFSL 60

Query: 61  KYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAV 120
           KY P+Y VP RYN FRCHSSLMT+QPLDP G+KAA+VALTRFCNVLGGCPPPV+K+VPAV
Sbjct: 61  KYPPSYVVPSRYNAFRCHSSLMTNQPLDPSGMKAAIVALTRFCNVLGGCPPPVVKMVPAV 120

Query: 121 CIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDP 180
            I++FAVWGLGPFLRYTRSLL   SD+NWKKS+TYKVMTLYLQPLLLW G  LICRALDP
Sbjct: 121 SIMIFAVWGLGPFLRYTRSLL--HSDSNWKKSQTYKVMTLYLQPLLLWTGATLICRALDP 180

Query: 181 VVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFA 240
           VVLPT+ SQVVKQR+LNFVRSLSTVLAFAYCLSSMI+QAQKFFSET+ESSDARNMGFQFA
Sbjct: 181 VVLPTESSQVVKQRVLNFVRSLSTVLAFAYCLSSMIQQAQKFFSETSESSDARNMGFQFA 240

Query: 241 WKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300
           WKAVYSAVWVAA+SLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP
Sbjct: 241 WKAVYSAVWVAALSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300

Query: 301 FIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGW 360
           F+VNEWIQT+IEGYEVSGTVE                                   HVGW
Sbjct: 301 FVVNEWIQTRIEGYEVSGTVE-----------------------------------HVGW 360

Query: 361 WSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK 420
           WSPTIIRGEDREAVHIPNH+FT+NVVRNLSQKTHWRIKTHLAISHLDV+KINNIVADMRK
Sbjct: 361 WSPTIIRGEDREAVHIPNHKFTMNVVRNLSQKTHWRIKTHLAISHLDVSKINNIVADMRK 420

Query: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI 480
           VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAI LDLLRVI
Sbjct: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIFLDLLRVI 480

Query: 481 RHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQ 540
           RHHRARLATPIRT+QKMHSDSDLESVPFSDSIFG GG  LNRRMLMIEPPYKVYG+DRKQ
Sbjct: 481 RHHRARLATPIRTVQKMHSDSDLESVPFSDSIFGHGGATLNRRMLMIEPPYKVYGDDRKQ 540

Query: 541 SHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMS 600
           SHSRTSRTTGEQNGKPIARSSGD+KAAKE TPS+RKTEV+ GE +DSD K H KVP S S
Sbjct: 541 SHSRTSRTTGEQNGKPIARSSGDNKAAKETTPSERKTEVKPGEGRDSDAKIHSKVPMSTS 600

Query: 601 DDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDP-STKQSENGSGST-K 660
           +D  S+ESKHKSSSRS SSTNGI+ MP+SDAKTT SD DNS +D  S+K+S+N SGS  K
Sbjct: 601 EDKPSSESKHKSSSRSVSSTNGIADMPTSDAKTTPSDADNSMQDSGSSKKSKNSSGSNNK 660

Query: 661 QNYKSNHPAVSLPEDVKKTGGS-SATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALDG 720
           QNYK  H +VS  EDVKK  G+ S+ SQPRIEG+QT    PST KPGVEEN+ILGVALDG
Sbjct: 661 QNYKPIHSSVSFQEDVKKPEGTTSSASQPRIEGDQTT--NPSTTKPGVEENIILGVALDG 711

Query: 721 SKRTLPIEEDMPSSPETV---KDLAAR-WNGNGTNGATTPDKDTKDQTPP 763
           SKRTLPIE+D PSSP+T    KDLAA   NGNG  GATTPDK+TK  +PP
Sbjct: 721 SKRTLPIEDDTPSSPQTTLGAKDLAAASLNGNGATGATTPDKETKRLSPP 711

BLAST of MC10g0502 vs. ExPASy TrEMBL
Match: A0A1S3BUC5 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493276 PE=4 SV=1)

HSP 1 Score: 1122 bits (2901), Expect = 0.0
Identity = 608/764 (79.58%), Postives = 653/764 (85.47%), Query Frame = 0

Query: 1   MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSM 60
           MV+VGSLQLSYHLGPWRN L+EENLKF +QS  IRLL+ AS S  LFQ K+TWSTHLFSM
Sbjct: 1   MVLVGSLQLSYHLGPWRNYLHEENLKFTSQSYGIRLLNVASSSSLLFQ-KDTWSTHLFSM 60

Query: 61  KYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAV 120
           KY PNYTVP RYNVFRC SSLMT+QPLD PG+KA++V LTRFCNVLGGCPP VIKLVP V
Sbjct: 61  KYPPNYTVPSRYNVFRCQSSLMTNQPLDLPGMKASIVTLTRFCNVLGGCPPSVIKLVPPV 120

Query: 121 CIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDP 180
           CII+FAVWGLGPFLR+TRSL    +D+NWKKSRTY VMTLYLQPLLLW G  LICRALDP
Sbjct: 121 CIIIFAVWGLGPFLRHTRSLF--HNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDP 180

Query: 181 VVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFA 240
           VVL T+ SQVVKQR+LNFVRSLSTVLA AYCLSSMI+QAQKFFSE TESSDARNMGFQFA
Sbjct: 181 VVLRTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSENTESSDARNMGFQFA 240

Query: 241 WKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300
           WKAVYSAVWVAA+SLFMELLGFSTQK LTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP
Sbjct: 241 WKAVYSAVWVAAISLFMELLGFSTQKLLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300

Query: 301 FIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGW 360
           F+VNEWIQTKIEGYEVSGTVEVCMF+LN    ++LMS                  +HVGW
Sbjct: 301 FVVNEWIQTKIEGYEVSGTVEVCMFLLNMYH-KLLMS-----------------KKHVGW 360

Query: 361 WSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK 420
           WSPTIIRGEDREAVHIPNHQFT+NVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK
Sbjct: 361 WSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK 420

Query: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI 480
           VLAKNPQVEQQRLHRRVFLENV+ ENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI
Sbjct: 421 VLAKNPQVEQQRLHRRVFLENVDSENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI 480

Query: 481 RHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQ 540
           RHH ARLATPIRTMQKMHSDSDLESVPFSDSIFG  G  LNRRMLMIEPPYKV+GEDRKQ
Sbjct: 481 RHHGARLATPIRTMQKMHSDSDLESVPFSDSIFGHRGATLNRRMLMIEPPYKVHGEDRKQ 540

Query: 541 SHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMS 600
           SHSRTSRTTGEQNGKPIARSSGDSKAAKE   SDRKTEV+TG   D+DTKKH KV  S+S
Sbjct: 541 SHSRTSRTTGEQNGKPIARSSGDSKAAKETMSSDRKTEVKTGG--DADTKKHSKVSMSVS 600

Query: 601 DDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQN 660
           +D SSNE KHK SS+SA+ST   S MP+SDAK T+SD DNS ED S KQSE+  GS  QN
Sbjct: 601 EDKSSNELKHKPSSKSAAST---SDMPTSDAKPTKSDADNSLEDSSPKQSEDSFGSNMQN 660

Query: 661 YKSNHPAVSLPEDVKKTGG--SSATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALDGS 720
           +K + PAVS PEDVKK GG  S+A SQ RI GEQT V  PST KPGVEEN+ILGVALDG 
Sbjct: 661 FKPSLPAVSSPEDVKKPGGTTSAAASQQRIVGEQTTVPNPSTKKPGVEENIILGVALDGP 720

Query: 721 KRTLPIEEDMPSSPETVKDLAARWNGNGTNGATTPDKDTKDQTP 762
           KRTLPIE+++P++    KDLA        NGAT  DK+TK Q+P
Sbjct: 721 KRTLPIEDNLPTAASGAKDLAV------FNGATMADKNTKCQSP 732

BLAST of MC10g0502 vs. TAIR 10
Match: AT5G10490.1 (MSCS-like 2 )

HSP 1 Score: 677.2 bits (1746), Expect = 1.6e-194
Identity = 400/766 (52.22%), Postives = 490/766 (63.97%), Query Frame = 0

Query: 1   MVIVGSLQLSYHLGPWRNRLYEENLKFATQSNNIRLLSAASPSPRLFQQKNTWSTHLFSM 60
           M + G+LQLS+ LG  RN+ +  N + +     + + +          Q    +  L + 
Sbjct: 1   MALYGTLQLSHSLGLCRNQRF-CNPENSAMRRRLHISNGPLSLGVPLGQHGFSNILLSNY 60

Query: 61  KYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAV 120
              P  +VP R   FRCHS   + + ++ P +KA  V LT+   ++    P V KLVPAV
Sbjct: 61  LRRPICSVPCRTTAFRCHSFSASGKAIE-PAVKAVTVVLTKSHGLMQQF-PFVYKLVPAV 120

Query: 121 CIIMFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDP 180
            +++F++WGL PF R  R++LL+ +DN WKKS TY VMT Y+QPLLLW G   ICRALDP
Sbjct: 121 ALLVFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDP 180

Query: 181 VVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFA 240
           VVLPT+ S++VK R+LNFVRSLSTVLAFAYCLSS+I+Q QK FSET+  SD RNMGFQFA
Sbjct: 181 VVLPTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFA 240

Query: 241 WKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRP 300
            KA+YSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRP
Sbjct: 241 GKALYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRP 300

Query: 301 FIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGW 360
           F++NEWIQTKIEGYEVSGTVE                                   HVGW
Sbjct: 301 FVLNEWIQTKIEGYEVSGTVE-----------------------------------HVGW 360

Query: 361 WSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRK 420
           WSPTIIRGEDREA+HIPNH+FT+NVVRNL+QKTHWRIKTHLAISHLDVNKINNIVADMRK
Sbjct: 361 WSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIKTHLAISHLDVNKINNIVADMRK 420

Query: 421 VLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVI 480
           VLAKNP VEQQRLHRRVFLENV PENQAL ILISCFVKTSH EEYL VKEAI+LDLLRVI
Sbjct: 421 VLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVKTSHHEEYLGVKEAILLDLLRVI 480

Query: 481 RHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQ 540
            HHRARLATPIRT++KM++++D+E+ PF +S++  GGV   R +++IEP YK+ GED+ +
Sbjct: 481 SHHRARLATPIRTIRKMYTETDVENTPFGESMY--GGVTSRRPLMLIEPAYKINGEDKSK 540

Query: 541 SHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMS 600
           S +R ++ T EQ  K      G +  +KE +  D K  V+ GE   SDT K P+      
Sbjct: 541 SQNRAAKPTAEQENK------GSNPKSKETSSPDLKANVKVGESPVSDTNKVPE------ 600

Query: 601 DDNSSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQN 660
                                          +T       +   P T +    SG+ K  
Sbjct: 601 -------------------------------ETVAKPVIKAVSKPPTPKDTETSGTEKPK 660

Query: 661 YKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALDGSKR 720
                         K++GG+  +++       T     STL    EEN++LGVAL+GSKR
Sbjct: 661 -------------AKRSGGTIKSTKTDETDSSTSSASRSTL----EENIVLGVALEGSKR 666

Query: 721 TLPIEEDMPSSP-ETVKDLAARWNGNGTNGATTPDKDTKDQTPPPS 766
           TLPIEE++ S P ET          +G NG    DK+ KD    P+
Sbjct: 721 TLPIEEEIHSPPMETDAKELTGARRSGGNGPLVADKEQKDSQSQPN 666

BLAST of MC10g0502 vs. TAIR 10
Match: AT5G10490.2 (MSCS-like 2 )

HSP 1 Score: 671.8 bits (1732), Expect = 6.6e-193
Identity = 387/703 (55.05%), Postives = 465/703 (66.15%), Query Frame = 0

Query: 64  PNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAVCII 123
           P  +VP R   FRCHS   + + ++ P +KA  V LT+   ++    P V KLVPAV ++
Sbjct: 35  PICSVPCRTTAFRCHSFSASGKAIE-PAVKAVTVVLTKSHGLMQQF-PFVYKLVPAVALL 94

Query: 124 MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 183
           +F++WGL PF R  R++LL+ +DN WKKS TY VMT Y+QPLLLW G   ICRALDPVVL
Sbjct: 95  VFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVL 154

Query: 184 PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 243
           PT+ S++VK R+LNFVRSLSTVLAFAYCLSS+I+Q QK FSET+  SD RNMGFQFA KA
Sbjct: 155 PTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKA 214

Query: 244 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 303
           +YSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRPF++
Sbjct: 215 LYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVL 274

Query: 304 NEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGWWSP 363
           NEWIQTKIEGYEVSGTVE                                   HVGWWSP
Sbjct: 275 NEWIQTKIEGYEVSGTVE-----------------------------------HVGWWSP 334

Query: 364 TIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLA 423
           TIIRGEDREA+HIPNH+FT+NVVRNL+QKTHWRIKTHLAISHLDVNKINNIVADMRKVLA
Sbjct: 335 TIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIKTHLAISHLDVNKINNIVADMRKVLA 394

Query: 424 KNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHH 483
           KNP VEQQRLHRRVFLENV PENQAL ILISCFVKTSH EEYL VKEAI+LDLLRVI HH
Sbjct: 395 KNPMVEQQRLHRRVFLENVIPENQALSILISCFVKTSHHEEYLGVKEAILLDLLRVISHH 454

Query: 484 RARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHS 543
           RARLATPIRT++KM++++D+E+ PF +S++  GGV   R +++IEP YK+ GED+ +S +
Sbjct: 455 RARLATPIRTIRKMYTETDVENTPFGESMY--GGVTSRRPLMLIEPAYKINGEDKSKSQN 514

Query: 544 RTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDN 603
           R ++ T EQ  K      G +  +KE +  D K  V+ GE   SDT K P+         
Sbjct: 515 RAAKPTAEQENK------GSNPKSKETSSPDLKANVKVGESPVSDTNKVPE--------- 574

Query: 604 SSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQNYKS 663
                                       +T       +   P T +    SG+ K     
Sbjct: 575 ----------------------------ETVAKPVIKAVSKPPTPKDTETSGTEKPK--- 634

Query: 664 NHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALDGSKRTLP 723
                      K++GG+  +++       T     STL    EEN++LGVAL+GSKRTLP
Sbjct: 635 ----------AKRSGGTIKSTKTDETDSSTSSASRSTL----EENIVLGVALEGSKRTLP 638

Query: 724 IEEDMPSSP-ETVKDLAARWNGNGTNGATTPDKDTKDQTPPPS 766
           IEE++ S P ET          +G NG    DK+ KD    P+
Sbjct: 695 IEEEIHSPPMETDAKELTGARRSGGNGPLVADKEQKDSQSQPN 638

BLAST of MC10g0502 vs. TAIR 10
Match: AT5G10490.3 (MSCS-like 2 )

HSP 1 Score: 671.8 bits (1732), Expect = 6.6e-193
Identity = 387/703 (55.05%), Postives = 465/703 (66.15%), Query Frame = 0

Query: 64  PNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGGCPPPVIKLVPAVCII 123
           P  +VP R   FRCHS   + + ++ P +KA  V LT+   ++    P V KLVPAV ++
Sbjct: 61  PICSVPCRTTAFRCHSFSASGKAIE-PAVKAVTVVLTKSHGLMQQF-PFVYKLVPAVALL 120

Query: 124 MFAVWGLGPFLRYTRSLLLHGSDNNWKKSRTYKVMTLYLQPLLLWAGVALICRALDPVVL 183
           +F++WGL PF R  R++LL+ +DN WKKS TY VMT Y+QPLLLW G   ICRALDPVVL
Sbjct: 121 VFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVL 180

Query: 184 PTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETTESSDARNMGFQFAWKA 243
           PT+ S++VK R+LNFVRSLSTVLAFAYCLSS+I+Q QK FSET+  SD RNMGFQFA KA
Sbjct: 181 PTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKA 240

Query: 244 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFIV 303
           +YSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRPF++
Sbjct: 241 LYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVL 300

Query: 304 NEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAIDIQYRFYQLQHVGWWSP 363
           NEWIQTKIEGYEVSGTVE                                   HVGWWSP
Sbjct: 301 NEWIQTKIEGYEVSGTVE-----------------------------------HVGWWSP 360

Query: 364 TIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLA 423
           TIIRGEDREA+HIPNH+FT+NVVRNL+QKTHWRIKTHLAISHLDVNKINNIVADMRKVLA
Sbjct: 361 TIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIKTHLAISHLDVNKINNIVADMRKVLA 420

Query: 424 KNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHH 483
           KNP VEQQRLHRRVFLENV PENQAL ILISCFVKTSH EEYL VKEAI+LDLLRVI HH
Sbjct: 421 KNPMVEQQRLHRRVFLENVIPENQALSILISCFVKTSHHEEYLGVKEAILLDLLRVISHH 480

Query: 484 RARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMIEPPYKVYGEDRKQSHS 543
           RARLATPIRT++KM++++D+E+ PF +S++  GGV   R +++IEP YK+ GED+ +S +
Sbjct: 481 RARLATPIRTIRKMYTETDVENTPFGESMY--GGVTSRRPLMLIEPAYKINGEDKSKSQN 540

Query: 544 RTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEVRTGEEKDSDTKKHPKVPTSMSDDN 603
           R ++ T EQ  K      G +  +KE +  D K  V+ GE   SDT K P+         
Sbjct: 541 RAAKPTAEQENK------GSNPKSKETSSPDLKANVKVGESPVSDTNKVPE--------- 600

Query: 604 SSNESKHKSSSRSASSTNGISGMPSSDAKTTRSDTDNSFEDPSTKQSENGSGSTKQNYKS 663
                                       +T       +   P T +    SG+ K     
Sbjct: 601 ----------------------------ETVAKPVIKAVSKPPTPKDTETSGTEKPK--- 660

Query: 664 NHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKPSTLKPGVEENLILGVALDGSKRTLP 723
                      K++GG+  +++       T     STL    EEN++LGVAL+GSKRTLP
Sbjct: 661 ----------AKRSGGTIKSTKTDETDSSTSSASRSTL----EENIVLGVALEGSKRTLP 664

Query: 724 IEEDMPSSP-ETVKDLAARWNGNGTNGATTPDKDTKDQTPPPS 766
           IEE++ S P ET          +G NG    DK+ KD    P+
Sbjct: 721 IEEEIHSPPMETDAKELTGARRSGGNGPLVADKEQKDSQSQPN 664

BLAST of MC10g0502 vs. TAIR 10
Match: AT1G58200.1 (MSCS-like 3 )

HSP 1 Score: 594.0 bits (1530), Expect = 1.8e-169
Identity = 352/691 (50.94%), Postives = 443/691 (64.11%), Query Frame = 0

Query: 49  QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 108
           +++ WS  L          V  R N F C S+L      + P +K+  V  TR  + LGG
Sbjct: 48  RQDVWSLQLLESLSGSIVPVSSRCNAFVCRSALSPGNGNEGPILKSTAVIFTRVYDALGG 107

Query: 109 CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLH-GSDNNWKKSRTYKVMTLYLQPLLL 168
             P ++KL+PAV I+ FA WGL P LR  R+ L   G+D N +KS T  ++  YLQPLLL
Sbjct: 108 -NPHLVKLIPAVGILAFATWGLRPLLRLARTTLFEKGNDANSQKSSTQYIVVSYLQPLLL 167

Query: 169 WAGVALICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETT 228
           W+G  L+CR LDP+VLP+   Q +KQR+L F RS+STVLAF+ CLSS+++Q QKFF ET 
Sbjct: 168 WSGAILLCRTLDPIVLPSSAGQAIKQRLLIFARSISTVLAFSCCLSSLLQQVQKFFMETN 227

Query: 229 ESSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFT 288
             +D RNMGF FA KAVY+A WVAA SLFMELLGFSTQKWLTAGGLGTVLLTLAGREI T
Sbjct: 228 NPADTRNMGFSFAGKAVYTAAWVAAASLFMELLGFSTQKWLTAGGLGTVLLTLAGREILT 287

Query: 289 NFLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAID 348
           NFLSS+MIHATRPF++NEWIQTKI GYEVSGTVE                          
Sbjct: 288 NFLSSIMIHATRPFVLNEWIQTKIGGYEVSGTVE-------------------------- 347

Query: 349 IQYRFYQLQHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLD 408
                     VGWWSPTIIRG+DREAVHIPNHQF++N+VRNL+QKTHWRIKTHLAISHLD
Sbjct: 348 ---------QVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWRIKTHLAISHLD 407

Query: 409 VNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLC 468
           V+KINNIVADMRKVL+KNPQ+EQQ++HRRVFLE+++PENQAL ILISCFVKTS FEEYLC
Sbjct: 408 VSKINNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALRILISCFVKTSRFEEYLC 467

Query: 469 VKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMI 528
           VKEA++LDLL VIRHH ARLATPIRT+Q+M +++++++  FSD +F +    +NRR ++I
Sbjct: 468 VKEAVLLDLLTVIRHHGARLATPIRTVQRMRNEAEVDTAGFSDIVFNQAA--MNRRYMLI 527

Query: 529 EPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEV-RTGEEKD 588
           EP YK+  +D  +S    S + G+++  P  +S    +   +  PS+ K E    G    
Sbjct: 528 EPSYKINSDDNSKS---PSPSPGQKSPSPGQKS---EERDLQEEPSETKAETENNGSVPV 587

Query: 589 SDTKKHPKVPTSMSDDNSSNESKHKSSS------RSASSTNGISGMPSSDAKTTRSDTDN 648
           S+ KK  +     S+ N+  +    S+S      +S        G P    K   SD + 
Sbjct: 588 SNAKKENQKAALGSNSNTGTKGSSTSTSDQPVAQKSEEKKKESVGDPHKAEKDEVSDDEA 647

Query: 649 SFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKPST 708
           + E   T +S+   GS K N +S            + GG S TS                
Sbjct: 648 TIE--QTLKSKAKQGSEKNNGESK----------ARDGGGSGTSSL-------------- 664

Query: 709 LKPGVEENLILGVALDGSKRTLPIEEDMPSS 732
               +EENL+LGVALDGSKRTLPI+E+  +S
Sbjct: 708 ----LEENLVLGVALDGSKRTLPIDEEHKAS 664

BLAST of MC10g0502 vs. TAIR 10
Match: AT1G58200.2 (MSCS-like 3 )

HSP 1 Score: 594.0 bits (1530), Expect = 1.8e-169
Identity = 352/691 (50.94%), Postives = 443/691 (64.11%), Query Frame = 0

Query: 49  QKNTWSTHLFSMKYSPNYTVPFRYNVFRCHSSLMTDQPLDPPGIKAAVVALTRFCNVLGG 108
           +++ WS  L          V  R N F C S+L      + P +K+  V  TR  + LGG
Sbjct: 48  RQDVWSLQLLESLSGSIVPVSSRCNAFVCRSALSPGNGNEGPILKSTAVIFTRVYDALGG 107

Query: 109 CPPPVIKLVPAVCIIMFAVWGLGPFLRYTRSLLLH-GSDNNWKKSRTYKVMTLYLQPLLL 168
             P ++KL+PAV I+ FA WGL P LR  R+ L   G+D N +KS T  ++  YLQPLLL
Sbjct: 108 -NPHLVKLIPAVGILAFATWGLRPLLRLARTTLFEKGNDANSQKSSTQYIVVSYLQPLLL 167

Query: 169 WAGVALICRALDPVVLPTKPSQVVKQRILNFVRSLSTVLAFAYCLSSMIEQAQKFFSETT 228
           W+G  L+CR LDP+VLP+   Q +KQR+L F RS+STVLAF+ CLSS+++Q QKFF ET 
Sbjct: 168 WSGAILLCRTLDPIVLPSSAGQAIKQRLLIFARSISTVLAFSCCLSSLLQQVQKFFMETN 227

Query: 229 ESSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFT 288
             +D RNMGF FA KAVY+A WVAA SLFMELLGFSTQKWLTAGGLGTVLLTLAGREI T
Sbjct: 228 NPADTRNMGFSFAGKAVYTAAWVAAASLFMELLGFSTQKWLTAGGLGTVLLTLAGREILT 287

Query: 289 NFLSSVMIHATRPFIVNEWIQTKIEGYEVSGTVEVCMFVLNHAPLEVLMSLETSYMNAID 348
           NFLSS+MIHATRPF++NEWIQTKI GYEVSGTVE                          
Sbjct: 288 NFLSSIMIHATRPFVLNEWIQTKIGGYEVSGTVE-------------------------- 347

Query: 349 IQYRFYQLQHVGWWSPTIIRGEDREAVHIPNHQFTINVVRNLSQKTHWRIKTHLAISHLD 408
                     VGWWSPTIIRG+DREAVHIPNHQF++N+VRNL+QKTHWRIKTHLAISHLD
Sbjct: 348 ---------QVGWWSPTIIRGDDREAVHIPNHQFSVNIVRNLTQKTHWRIKTHLAISHLD 407

Query: 409 VNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVNPENQALLILISCFVKTSHFEEYLC 468
           V+KINNIVADMRKVL+KNPQ+EQQ++HRRVFLE+++PENQAL ILISCFVKTS FEEYLC
Sbjct: 408 VSKINNIVADMRKVLSKNPQIEQQKIHRRVFLEDIDPENQALRILISCFVKTSRFEEYLC 467

Query: 469 VKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGRGGVNLNRRMLMI 528
           VKEA++LDLL VIRHH ARLATPIRT+Q+M +++++++  FSD +F +    +NRR ++I
Sbjct: 468 VKEAVLLDLLTVIRHHGARLATPIRTVQRMRNEAEVDTAGFSDIVFNQAA--MNRRYMLI 527

Query: 529 EPPYKVYGEDRKQSHSRTSRTTGEQNGKPIARSSGDSKAAKEATPSDRKTEV-RTGEEKD 588
           EP YK+  +D  +S    S + G+++  P  +S    +   +  PS+ K E    G    
Sbjct: 528 EPSYKINSDDNSKS---PSPSPGQKSPSPGQKS---EERDLQEEPSETKAETENNGSVPV 587

Query: 589 SDTKKHPKVPTSMSDDNSSNESKHKSSS------RSASSTNGISGMPSSDAKTTRSDTDN 648
           S+ KK  +     S+ N+  +    S+S      +S        G P    K   SD + 
Sbjct: 588 SNAKKENQKAALGSNSNTGTKGSSTSTSDQPVAQKSEEKKKESVGDPHKAEKDEVSDDEA 647

Query: 649 SFEDPSTKQSENGSGSTKQNYKSNHPAVSLPEDVKKTGGSSATSQPRIEGEQTPVLKPST 708
           + E   T +S+   GS K N +S            + GG S TS                
Sbjct: 648 TIE--QTLKSKAKQGSEKNNGESK----------ARDGGGSGTSSL-------------- 664

Query: 709 LKPGVEENLILGVALDGSKRTLPIEEDMPSS 732
               +EENL+LGVALDGSKRTLPI+E+  +S
Sbjct: 708 ----LEENLVLGVALDGSKRTLPIDEEHKAS 664

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q56X462.2e-19352.22Mechanosensitive ion channel protein 2, chloroplastic OS=Arabidopsis thaliana OX... [more]
Q8L7W12.5e-16850.94Mechanosensitive ion channel protein 3, chloroplastic OS=Arabidopsis thaliana OX... [more]
P0AEB65.2e-1726.83Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=833... [more]
P0AEB55.2e-1726.83Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) O... [more]
Q585434.4e-0821.63Large-conductance mechanosensitive channel MscMJLR OS=Methanocaldococcus jannasc... [more]
Match NameE-valueIdentityDescription
XP_022136725.10.095.43mechanosensitive ion channel protein 2, chloroplastic-like [Momordica charantia][more]
KAG6577342.10.080.26Mechanosensitive ion channel protein 2, chloroplastic, partial [Cucurbita argyro... [more]
XP_023552794.10.079.64mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita... [more]
XP_022929450.10.079.87mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucurbita... [more]
KAG7015430.10.080.23Mechanosensitive ion channel protein 2, chloroplastic [Cucurbita argyrosperma su... [more]
Match NameE-valueIdentityDescription
A0A6J1C4S50.095.43mechanosensitive ion channel protein 2, chloroplastic-like OS=Momordica charanti... [more]
A0A6J1ES610.079.87mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 OS=Cucurbi... [more]
A0A6J1JBP80.078.99mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 OS=Cucurbi... [more]
A0A6J1EN690.078.31mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 OS=Cucurbi... [more]
A0A1S3BUC50.079.58mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 OS=Cucumis... [more]
Match NameE-valueIdentityDescription
AT5G10490.11.6e-19452.22MSCS-like 2 [more]
AT5G10490.26.6e-19355.05MSCS-like 2 [more]
AT5G10490.36.6e-19355.05MSCS-like 2 [more]
AT1G58200.11.8e-16950.94MSCS-like 3 [more]
AT1G58200.21.8e-16950.94MSCS-like 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.287.1260coord: 195..299
e-value: 8.9E-8
score: 34.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 744..758
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 676..690
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 610..635
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 568..597
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 740..766
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 533..701
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 645..666
NoneNo IPR availablePANTHERPTHR43634:SF6MECHANOSENSITIVE ION CHANNEL PROTEIN 2, CHLOROPLASTICcoord: 356..759
NoneNo IPR availablePANTHERPTHR43634:SF6MECHANOSENSITIVE ION CHANNEL PROTEIN 2, CHLOROPLASTICcoord: 1..322
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 362..484
e-value: 9.7E-11
score: 41.6
coord: 243..321
e-value: 1.5E-10
score: 41.0
IPR045042Mechanosensitive ion channel protein YnaI-likePANTHERPTHR43634OW CONDUCTANCE MECHANOSENSITIVE CHANNELcoord: 356..759
IPR045042Mechanosensitive ion channel protein YnaI-likePANTHERPTHR43634OW CONDUCTANCE MECHANOSENSITIVE CHANNELcoord: 1..322

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0502.1MC10g0502.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006811 ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane