MC10g0487 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0487
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionprotein NRT1/ PTR FAMILY 4.3-like
LocationMC10: 3949414 .. 3954163 (+)
RNA-Seq ExpressionMC10g0487
SyntenyMC10g0487
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGATTGTTATCCACATGTTTGTAGTGCGTGTGGGTATGGAATTAATTTTGCCATTGAATCTTAAATTTTGCCCAAAGAATTAAAATGGGAAAAAAAAAATAAAAAAGGGACCTTTGATTTGATAAAAGAAAACAATAATACTAATAATTTAGAATAAGAAAAATGGGATGGGTGGAGAGAGGTGGGGGCCTTTGCAGTTTATTTTGGCTTTATAAATGGAATGCCCAACCAGAAGAAGCTGCCTCACATTTCAGCAGAAAAATAGTCATATTACCCTTCTAGTAATTTCTCATTAACAAAATTTCCCTCCATTTTAATTCTCTGTTTTGGTTCAAGGGAAAGAAAAATAATGGAAATGGAAATGGAAAGAAGGAAGGATTTCAAAGGGGATGAAGATCGTGATCATGTCACTGTTGATTGGAGAGGAAGACCCTCCAATCCCCTCTCCCATGGTGGAATGAGAGCAGCTGCTTTTGTTCTTGGTAAATTTCTCTCTCTACATCTCGTGTTTTTCGTTTCCATTCTCGATTTCTAGTTCGTTTGGAGTTGAGGAATATACTTGTACACGAGTTAGGAGGAGTTCTTGCTAATTTTTTGATTTGACTTATTCTTAGTGGTTCAGTGAGAATTACTCATCTATTAAATGCAACGCATACCGTTGATGAAAATGAAGCGGTGAGGCTTCAATGAGTTGAAAAGAAAACTTCTCTTTGACATCAATGGATGTGTGATGGATGTGTGATGTTTTTGAGAAAAGTGGTTTTCTACCAAGTAAATGGTTTTGGCCTTGAGAGAATATAAAATAAGGCATGAATAACAAAACATGAATAAAATAATGGAAAGACTTAGGTTTCCTTTTTAGAATATGAGATGGGTCTTGGAGAGAGAGAGCAAATAATAAATCTAATGGACTTGGCCAATGCTGCTTGATCTCTACTTTTTTCGCGAGTTCGTATGCTCGAAATTCTTGCTCTATATTTATGATGTAATATTTTTCAAATCGTGCAGGACTGCAAGCATTTGAGATAATGGCGATCGCTGCAGTCGGGAACAATCTGATAACATATGCGATAAATGAGATGCACTTTTCACTGTCAAAATCAGCAAACGTGGTCACAAACTTTGTTGGGACAGTGTTCCTTTTGGCTCTTGTTGGTGGTTTCCTTTCAGATTCTTATCTTGGCAGTTTTTGGACCATGCTCGTCTTTGGCTTTGTTGAACTTTCTGTAAGTTCCCATCATTTCATTTCCCCATAATTTCTTATTTTTCTTTATGATCAAAATCCACTTCTTTTCACTAATCTCAGTCTCTTAAAAATCATTCAACAAAGATACACCCATCAATCTATCATTCATTTCATGTTTATATATAATCAACAAGTTCAATGAATGATCACCTCCACTTTCTTTTCGGTATGAGATAAAAAGGTATTATTTTTAACACAAAGAGACCCAAGTCATCTGTTTTTCTCAAACATAATATGATTTATAAGTAAACATTATGTATCGAATCTAGCTAGAGATCTTATGATCAAACCACTGTAATGTTAAAAATATTAACTTCGTGGTTTAACATGCTTTCAGACCAACAGATCTATAAATATTGATATTCACTTTCGTAACCCACCAGTTTAAGCTCTTGAGTTCAGTGTTGAGATTATCATATACATACATACATCTAAAACCTCAAGAAAAGACAAGTTTTTTGCATGTGCATGCATAGATCACATGATAAATCATTATCTTGTAACATTCTCTCTCTCTACCACTTTGAAGAGAGAGAGAGAGCAAAACCGAAATGCTAAAAGTGAAAGAAATGAAATTATTGTGGCAGGGATTCATATTACTGTCAGTTCAAGCTCATCTGCCCCAACTAAAGCCACCACAATGCAATATGCTAAGTGAAGATGGGGAGAATTGTGAGGAAGCCAAAGGGTTAAAGGCATTGATATTCTTTGTGGCACTGTATTTGGTGGCCTTAGGGAGTGGGTGTGTGAAACCCAACATGATAGCTCATGGGGCTGACCAATTTAGCCCACAAAATAACCCAAAGCACTCAAAAAAGTTGTCAACTTATTTCAATGCTGCATATTTTGCCTTCTCTGTGGGAGAGCTCATTGCCCTCACTCTTGTGGTGTGGATCCAAACTCATTCTGGGATGGATGTTGGATTTGGAGTCTCAGCTGCTGTTATGGCCATGGGATTGATCTCTTTGGTTGGGGGGATTTTTTACTACAGGAATAAACCTCCTCAAGGACCCATTTTCACCCCTATTCTTCAGGTGGTTTTTCTTCTCTTTTTACCTTCTTTCTTTGCAATTTTTACCTTGTGTTGTGTTGTGTTGTGCACAGTTGGGAGAGAAATTACCCTTCACAAAGCTTTCTTTTCTTACTTTCTTTTGTTTTTTTGTTTTTTTTGCTTTCTTGTAGTTATTATCTCAGTCTAAACCCAATTTCATTTTGTATACTTTCAGATGTTCTATTTTAACTCTTATACTTTTGTGTCGTTGTGTTCTTTGTCTTTGATTTTACGTAAAAGTATGTTTTTAGATCTTCTCGTCTATTTTTTTGTGTTGTGTTCTTAAGACCCAGAAGTTTAAACCGCCATTTGATCATATGTAAGATTCCTGCTAAATAAAATTTATATACCAAAAAAAGGCCTAAACAGGCCACTTTGATGTTAAAAAAGAATTGTAAGAGTAGAACCAGTTGAGGGTTTTAAAATAGATTTTCAATGAAAATTTGAGAGTATTTTTTTTTTTTTTTTAAGGTTGTAGTGGGAAGACTGTTGGGAAAGTACTGTTTGGGATGTTTGTTATGTTTGGATTAAAAAGGAGTGACATCTATGTATGGATTGAATGGATATATATTTGAATTGAAAAGACATAAAAGTTAGTGCTGTCTGCTGAGTCATATTGTCCTGAAAAAGTTCATTCAAACTTCTTTCTTTCTCTCTCTCTCTCTCATACTAATCCAATTAATTACTGTAAAAACAAATACATGTTCAGGTTTTTGTTGCTGCTCTTTTGAAGAGAAAGCAAGTTTGTCCTTCCAATCCTCATATGCTTCATGGAAGCCAAAACAATGTTGATCATAAAGTTGCTTTATCCTCTGATTCTGCCAACCTTATTCCCACTCAAAAGTTCAGGCAAGTCTAGTTCGATTCCAATTGTAAATATGGACATCATTAGCTCCCTCCGATAACGATTCTGTTTTTAATTTCCTCTTTTTAAAAATTATGTTTGTTTTCTCACAATTTCGTTGGTATGTTTTTCAGCTTTCTTCATCATACATTTGAATTCTTGACCAAAATTTTAAAATAAAAACAAGTTTTTGTAAACTTAACTTTTAAAAATAAAATAGTTATCAACCGGCATCAGTGATCTTAATGAAATGAAGTTTCCTTAATATGATAGGATGTTGAAGTTGGTACTAAAATTTGGATTGAATTTCAGGTTCCTGGACAAGGCTTGTATCAAAAGCCAAGATGGGGAAACAAAGGAGAGCCCATGGAGATTGTGCACTCCCACTCAAGTTGAGCAAGTGAAGATTTTAATTTCAGTTGTCCCAATATTTGCTTGCACAATTATATTCAACACTATATTGGCTCAGCTCCAAACATTTTCAGTCCAGCAAGGGAGTTTGATGAACACTGAACTAACCAAATCCTTCCACATTCCTCCAGCTTCACTTCAAGCCATTCCTTACATTATGCTCATCTTCATCGTCCCTTTATACGACACTTTCTTCGTCCCATTTGCAAGAAAATTCACTGGCCACACCTCCGGAATCTCTCCCCTAAAACGCATCGGCTTTGGCCTCTTCCTATCGACATTTTCGATGGTTTCAGCAGCCATAATGGAGAAAAAGAGGAGAGATTCAGCTCTGAATTTGAACCAAACGGTATCGATCTTCTGGATCACCCCGCAATTCCTCATTTTCGGGTTATCGGAGATGTTCACAGCAGTTGGGCTCATCGAGTTCTTCTACAAGCAATCATCAAAAGGAATGCAAGCATTTTTAACAGCCATTACATACTGTTCATACTCTTTTGGATTCTACCTGAGCTCACTGTTAGTGTCAATGGTGAATAAGATCACAGCAACATCCTCCAATGGAGGTTGGCTCCATGACAATAACCTAAACAAAGACAGACTTGATCTCTTCTACTGGCTGTTGGCCATACTCAGCTTCCTAAATTTTCTTAACTATCTCTTCTGGTCAACATGGTTCGATGAACATCCATCCCTCTCACAGAAGCAACACCATGACGAAAATCCCCACCATGGAGAAGACTATCACAGTTATAATTTCAATTCTTCAAAGACCAATATCGACGAATATGTACCTTGATTAAATGATCTTTCACCATTTTTTCACCTTTTTTAGATGAAAAAGACGAATATGTACCTTGATTAAATGATCTTTCACCATTTTTTCACCTTTTTTAGATGAAAAAATCAATATAACCTAGGGGAGTCAATAGAAGGAATATAAGTAATCTACCTGATATATGTGTTTAGAATTTAGGGTTTAGGGTTTGCTGATATATGTACTTAGTGTTGTGTAGAGAAGAAACAAAATAATATTATAAGTAGAAATCCTATATATTATCCTCAAATGAATGTCAAAAGTTGCATTCCATCCATTGAAAAGAGGATCATTCTTTCTAGGAGAGGTTGGACTATAAAACCCTCTTACTTCCCAAAGGGTTTAAATTTCACTTTTATATATCAAATAATTGATTTACT

mRNA sequence

GAGATTGTTATCCACATGTTTGTAGTGCGTGTGGGTATGGAATTAATTTTGCCATTGAATCTTAAATTTTGCCCAAAGAATTAAAATGGGAAAAAAAAAATAAAAAAGGGACCTTTGATTTGATAAAAGAAAACAATAATACTAATAATTTAGAATAAGAAAAATGGGATGGGTGGAGAGAGGTGGGGGCCTTTGCAGTTTATTTTGGCTTTATAAATGGAATGCCCAACCAGAAGAAGCTGCCTCACATTTCAGCAGAAAAATAGTCATATTACCCTTCTAGTAATTTCTCATTAACAAAATTTCCCTCCATTTTAATTCTCTGTTTTGGTTCAAGGGAAAGAAAAATAATGGAAATGGAAATGGAAAGAAGGAAGGATTTCAAAGGGGATGAAGATCGTGATCATGTCACTGTTGATTGGAGAGGAAGACCCTCCAATCCCCTCTCCCATGGTGGAATGAGAGCAGCTGCTTTTGTTCTTGGACTGCAAGCATTTGAGATAATGGCGATCGCTGCAGTCGGGAACAATCTGATAACATATGCGATAAATGAGATGCACTTTTCACTGTCAAAATCAGCAAACGTGGTCACAAACTTTGTTGGGACAGTGTTCCTTTTGGCTCTTGTTGGTGGTTTCCTTTCAGATTCTTATCTTGGCAGTTTTTGGACCATGCTCGTCTTTGGCTTTGTTGAACTTTCTGGATTCATATTACTGTCAGTTCAAGCTCATCTGCCCCAACTAAAGCCACCACAATGCAATATGCTAAGTGAAGATGGGGAGAATTGTGAGGAAGCCAAAGGGTTAAAGGCATTGATATTCTTTGTGGCACTGTATTTGGTGGCCTTAGGGAGTGGGTGTGTGAAACCCAACATGATAGCTCATGGGGCTGACCAATTTAGCCCACAAAATAACCCAAAGCACTCAAAAAAGTTGTCAACTTATTTCAATGCTGCATATTTTGCCTTCTCTGTGGGAGAGCTCATTGCCCTCACTCTTGTGGTGTGGATCCAAACTCATTCTGGGATGGATGTTGGATTTGGAGTCTCAGCTGCTGTTATGGCCATGGGATTGATCTCTTTGGTTGGGGGGATTTTTTACTACAGGAATAAACCTCCTCAAGGACCCATTTTCACCCCTATTCTTCAGGTTTTTGTTGCTGCTCTTTTGAAGAGAAAGCAAGTTTGTCCTTCCAATCCTCATATGCTTCATGGAAGCCAAAACAATGTTGATCATAAAGTTGCTTTATCCTCTGATTCTGCCAACCTTATTCCCACTCAAAAGTTCCTGGACAAGGCTTGTATCAAAAGCCAAGATGGGGAAACAAAGGAGAGCCCATGGAGATTGTGCACTCCCACTCAAGTTGAGCAAGTGAAGATTTTAATTTCAGTTGTCCCAATATTTGCTTGCACAATTATATTCAACACTATATTGGCTCAGCTCCAAACATTTTCAGTCCAGCAAGGGAGTTTGATGAACACTGAACTAACCAAATCCTTCCACATTCCTCCAGCTTCACTTCAAGCCATTCCTTACATTATGCTCATCTTCATCGTCCCTTTATACGACACTTTCTTCGTCCCATTTGCAAGAAAATTCACTGGCCACACCTCCGGAATCTCTCCCCTAAAACGCATCGGCTTTGGCCTCTTCCTATCGACATTTTCGATGGTTTCAGCAGCCATAATGGAGAAAAAGAGGAGAGATTCAGCTCTGAATTTGAACCAAACGGTATCGATCTTCTGGATCACCCCGCAATTCCTCATTTTCGGGTTATCGGAGATGTTCACAGCAGTTGGGCTCATCGAGTTCTTCTACAAGCAATCATCAAAAGGAATGCAAGCATTTTTAACAGCCATTACATACTGTTCATACTCTTTTGGATTCTACCTGAGCTCACTGTTAGTGTCAATGGTGAATAAGATCACAGCAACATCCTCCAATGGAGGTTGGCTCCATGACAATAACCTAAACAAAGACAGACTTGATCTCTTCTACTGGCTGTTGGCCATACTCAGCTTCCTAAATTTTCTTAACTATCTCTTCTGGTCAACATGGTTCGATGAACATCCATCCCTCTCACAGAAGCAACACCATGACGAAAATCCCCACCATGGAGAAGACTATCACAGTTATAATTTCAATTCTTCAAAGACCAATATCGACGAATATGTACCTTGATTAAATGATCTTTCACCATTTTTTCACCTTTTTTAGATGAAAAAGACGAATATGTACCTTGATTAAATGATCTTTCACCATTTTTTCACCTTTTTTAGATGAAAAAATCAATATAACCTAGGGGAGTCAATAGAAGGAATATAAGTAATCTACCTGATATATGTGTTTAGAATTTAGGGTTTAGGGTTTGCTGATATATGTACTTAGTGTTGTGTAGAGAAGAAACAAAATAATATTATAAGTAGAAATCCTATATATTATCCTCAAATGAATGTCAAAAGTTGCATTCCATCCATTGAAAAGAGGATCATTCTTTCTAGGAGAGGTTGGACTATAAAACCCTCTTACTTCCCAAAGGGTTTAAATTTCACTTTTATATATCAAATAATTGATTTACT

Coding sequence (CDS)

ATGGAAATGGAAATGGAAAGAAGGAAGGATTTCAAAGGGGATGAAGATCGTGATCATGTCACTGTTGATTGGAGAGGAAGACCCTCCAATCCCCTCTCCCATGGTGGAATGAGAGCAGCTGCTTTTGTTCTTGGACTGCAAGCATTTGAGATAATGGCGATCGCTGCAGTCGGGAACAATCTGATAACATATGCGATAAATGAGATGCACTTTTCACTGTCAAAATCAGCAAACGTGGTCACAAACTTTGTTGGGACAGTGTTCCTTTTGGCTCTTGTTGGTGGTTTCCTTTCAGATTCTTATCTTGGCAGTTTTTGGACCATGCTCGTCTTTGGCTTTGTTGAACTTTCTGGATTCATATTACTGTCAGTTCAAGCTCATCTGCCCCAACTAAAGCCACCACAATGCAATATGCTAAGTGAAGATGGGGAGAATTGTGAGGAAGCCAAAGGGTTAAAGGCATTGATATTCTTTGTGGCACTGTATTTGGTGGCCTTAGGGAGTGGGTGTGTGAAACCCAACATGATAGCTCATGGGGCTGACCAATTTAGCCCACAAAATAACCCAAAGCACTCAAAAAAGTTGTCAACTTATTTCAATGCTGCATATTTTGCCTTCTCTGTGGGAGAGCTCATTGCCCTCACTCTTGTGGTGTGGATCCAAACTCATTCTGGGATGGATGTTGGATTTGGAGTCTCAGCTGCTGTTATGGCCATGGGATTGATCTCTTTGGTTGGGGGGATTTTTTACTACAGGAATAAACCTCCTCAAGGACCCATTTTCACCCCTATTCTTCAGGTTTTTGTTGCTGCTCTTTTGAAGAGAAAGCAAGTTTGTCCTTCCAATCCTCATATGCTTCATGGAAGCCAAAACAATGTTGATCATAAAGTTGCTTTATCCTCTGATTCTGCCAACCTTATTCCCACTCAAAAGTTCCTGGACAAGGCTTGTATCAAAAGCCAAGATGGGGAAACAAAGGAGAGCCCATGGAGATTGTGCACTCCCACTCAAGTTGAGCAAGTGAAGATTTTAATTTCAGTTGTCCCAATATTTGCTTGCACAATTATATTCAACACTATATTGGCTCAGCTCCAAACATTTTCAGTCCAGCAAGGGAGTTTGATGAACACTGAACTAACCAAATCCTTCCACATTCCTCCAGCTTCACTTCAAGCCATTCCTTACATTATGCTCATCTTCATCGTCCCTTTATACGACACTTTCTTCGTCCCATTTGCAAGAAAATTCACTGGCCACACCTCCGGAATCTCTCCCCTAAAACGCATCGGCTTTGGCCTCTTCCTATCGACATTTTCGATGGTTTCAGCAGCCATAATGGAGAAAAAGAGGAGAGATTCAGCTCTGAATTTGAACCAAACGGTATCGATCTTCTGGATCACCCCGCAATTCCTCATTTTCGGGTTATCGGAGATGTTCACAGCAGTTGGGCTCATCGAGTTCTTCTACAAGCAATCATCAAAAGGAATGCAAGCATTTTTAACAGCCATTACATACTGTTCATACTCTTTTGGATTCTACCTGAGCTCACTGTTAGTGTCAATGGTGAATAAGATCACAGCAACATCCTCCAATGGAGGTTGGCTCCATGACAATAACCTAAACAAAGACAGACTTGATCTCTTCTACTGGCTGTTGGCCATACTCAGCTTCCTAAATTTTCTTAACTATCTCTTCTGGTCAACATGGTTCGATGAACATCCATCCCTCTCACAGAAGCAACACCATGACGAAAATCCCCACCATGGAGAAGACTATCACAGTTATAATTTCAATTCTTCAAAGACCAATATCGACGAATATGTACCTTGA

Protein sequence

MEMEMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFILLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALSSDSANLIPTQKFLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEMFTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNSSKTNIDEYVP
Homology
BLAST of MC10g0487 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 825.1 bits (2130), Expect = 5.2e-238
Identity = 425/570 (74.56%), Postives = 484/570 (84.91%), Query Frame = 0

Query: 4   EMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLIT 63
           E E   +    E  +  +VDWRGRPSNP  HGGMRAA FVLGLQAFEIM IAAVGNNLIT
Sbjct: 12  EQEEVSNENNWELAEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLIT 71

Query: 64  YAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFILLS 123
           Y INEMHF LSK+AN+VTNFVGT+F+ AL+GG+LSD++LGSFWT+++FGFVELSGFILLS
Sbjct: 72  YVINEMHFPLSKAANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLS 131

Query: 124 VQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQF 183
           VQAHLPQLKPP+CN L +  + CEEAKG KA+IFF+ALYLVALGSGCVKPNMIAHGADQF
Sbjct: 132 VQAHLPQLKPPKCNPLID--QTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQF 191

Query: 184 SPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLIS 243
           S Q++PK SK+LS+YFNAAYFAFS+GELIALTL+VW+QTHSGMD+GFGVSAA M MG+IS
Sbjct: 192 S-QSHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIIS 251

Query: 244 LVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVA---LS 303
           LV G  Y+RNK P+  IFTPI  V VAA+LKRK   PS+P MLHG     DH VA   + 
Sbjct: 252 LVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKLASPSDPRMLHG-----DHHVANDVVP 311

Query: 304 SDSANLIPTQKFLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTI 363
           S +    P  +FLDKACIK QD  TKESPWRLCT TQVEQVK LIS+VPIFA TI+FNTI
Sbjct: 312 SSTLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTI 371

Query: 364 LAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHT 423
           LAQLQTFSVQQGS MNT L+ SFHIPPASLQAIPYIMLIF+VPLYD+F VPFARK TGH 
Sbjct: 372 LAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHN 431

Query: 424 SGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEMFT 483
           SGI PL RIG GLFLSTFSMVSAA++EKKRRDS++   + +SIFWITPQFLIFG+SEMFT
Sbjct: 432 SGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFT 491

Query: 484 AVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATS-SNGGWLHDNN 543
           AVGLIEFFYKQS+KGM++FL A+TYCSYSFGFY SS+LVS+VNKIT+TS  + GWL +N+
Sbjct: 492 AVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGEND 551

Query: 544 LNKDRLDLFYWLLAILSFLNFLNYLFWSTW 570
           LNKDRLDLFYWLLA+LS LNFL+YLFWS W
Sbjct: 552 LNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

BLAST of MC10g0487 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 771.5 bits (1991), Expect = 6.9e-222
Identity = 412/603 (68.33%), Postives = 481/603 (79.77%), Query Frame = 0

Query: 4   EMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLIT 63
           E +R      +E  D + VD+RGRP  P  HGG RAA FVLG QAFE+MAIAAVGNNLIT
Sbjct: 9   EAKRGVIHTSEESLDDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLIT 68

Query: 64  YAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFILLS 123
           Y  NEMHF LSKSAN+VTNF+GTVFLL+L+GGFLSDSYLGSF TMLVFG +E+SGFILLS
Sbjct: 69  YVFNEMHFPLSKSANLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLS 128

Query: 124 VQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQF 183
           VQAHLP+L+PP+CNM S    +C EA G KA   + AL LVALGSGC+KPN+I+HGA+QF
Sbjct: 129 VQAHLPELRPPECNMKSTT-IHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQF 188

Query: 184 SPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLIS 243
                 K  +KLS++FNAAYFAFS+G+LIALTL+VW+QTHSGMDVGFGVSAAVMA G+IS
Sbjct: 189 ----QRKDLRKLSSFFNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMIS 248

Query: 244 LVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLH-GSQNNVDHKVALSSD 303
           LV G  +YRNKPP G IFTPI QVFVAA+ KRKQ+CPSNP+M+H  S + V  K  L S+
Sbjct: 249 LVAGTSFYRNKPPSGSIFTPIAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKPLLHSN 308

Query: 304 SANLIPTQKFLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILA 363
                   +FLDKACIK+Q G+  ESPWRLCT  QV QVKIL+SV+PIFACTIIFNTILA
Sbjct: 309 KF------RFLDKACIKTQ-GKAMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILA 368

Query: 364 QLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSG 423
           QLQTFSVQQGS MNT +TK+F IPPASLQAIPYI+LIF VPLY+TFFVP ARK TG+ SG
Sbjct: 369 QLQTFSVQQGSSMNTHITKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSG 428

Query: 424 ISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEMFTAV 483
           ISPL+RIG GLFL+TFSMV+AA++EKKRR+S L  N  +SIFWI PQFLIFGLSEMFTAV
Sbjct: 429 ISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAV 488

Query: 484 GLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNG---GWLHDNN 543
           GL+EFFYKQSS+ MQ+FLTA+TYCSYSFGFYLSS+LVS VN++T+++ +G   GWL DN+
Sbjct: 489 GLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDND 548

Query: 544 LNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNS 603
           LNKDRLD FYWLLA LSF+NF NYLFWS W+   PS +   HH    +  E   +     
Sbjct: 549 LNKDRLDHFYWLLASLSFINFFNYLFWSRWYSCDPSAT---HHSAEVNSLEALENGEIKD 596

BLAST of MC10g0487 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 6.2e-114
Identity = 240/568 (42.25%), Postives = 346/568 (60.92%), Query Frame = 0

Query: 23  DWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSANVVTN 82
           DWR R +    HGGM AA+FVL ++  E +A  A  +NL+ Y    MH S SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 83  FVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFILLSVQAHLPQLKPPQCNMLSED 142
           F+GT FLLAL+GGFLSD++  +F   L+   +E  G I+L++QA  P L PP C     D
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSC-----D 134

Query: 143 GENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLSTYFNAA 202
              CEE  G KA + FV LYLVALG G +K ++ +HGA+QF  ++ PK  K+ ST+FN  
Sbjct: 135 SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFD-ESTPKGRKQRSTFFNYF 194

Query: 203 YFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGPIFT 262
            F  + G L+A+T VVW++ + G + GFGVS   + + ++  + G  +YRNK P G   T
Sbjct: 195 VFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLT 254

Query: 263 PILQVFVAALLK------------RKQVCPSNPHMLHGSQNNVDHKVALSSD-SANLIPT 322
            IL+V +AA +K               V PSN H +   +  V+ +  L        +P 
Sbjct: 255 TILKVLLAASVKCCSSGSSSNAVASMSVSPSN-HCVSKGKKEVESQGELEKPRQEEALPP 314

Query: 323 QKFLDKACIKSQDGETKESP-WRL--CTPTQVEQVKILISVVPIFACTIIFNTILAQLQT 382
           +  L  + +K  +G   E P  RL  CT  QVE VKI++ ++PIFACTI+ N  LAQL T
Sbjct: 315 RAQLTNS-LKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLST 374

Query: 383 FSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPL 442
           FSVQQ + MNT++  S  IPPASL   P + ++ + P+YD   +PFARK T   +G++ L
Sbjct: 375 FSVQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHL 434

Query: 443 KRIGFGLFLSTFSMVSAAIMEKKRRDSA-----LNLNQT--VSIFWITPQFLIFGLSEMF 502
           +RIG GL LS  +M  AA++E KR+  A     L+  +T  V+  WI  Q+L  G +++F
Sbjct: 435 QRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLF 494

Query: 503 TAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNN 562
           T  GL+E+F+ ++   M++  T++++ S + G+YLSS++VS+VN IT +S N  WL   +
Sbjct: 495 TLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKS 554

Query: 563 LNKDRLDLFYWLLAILSFLNFLNYLFWS 568
           +N+ +LD FYWL+ +LS  NFL+YLFW+
Sbjct: 555 INRYKLDYFYWLMCVLSAANFLHYLFWA 573

BLAST of MC10g0487 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 1.3e-108
Identity = 229/574 (39.90%), Postives = 344/574 (59.93%), Query Frame = 0

Query: 1   MEMEMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNN 60
           ME+EM       GD  +     DWR + +    HGGM AA+FVL ++  E +A  A  +N
Sbjct: 1   MEVEMH------GDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASN 60

Query: 61  LITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFI 120
           L+ Y  N MH SL++S++ VT F+ T FLLAL+GGFL+D++  +F   L+   +E  G I
Sbjct: 61  LVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLI 120

Query: 121 LLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGA 180
           LL++QA  P L PP C   S     CE   G KA   FV LYLV+LG G +K ++ +HGA
Sbjct: 121 LLTIQARRPSLMPPPCK--SSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGA 180

Query: 181 DQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMG 240
           +QF  +  PK  K+ ST+FN   F  S G L+A+T VVWI+ + G + GFGVS   + + 
Sbjct: 181 EQFD-EGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLS 240

Query: 241 LISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALS 300
           ++  + G  +Y+NK P+G   T I +V +AA +     C S     H +   V  +    
Sbjct: 241 ILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVS---CSSKTSSNHFTSREVQSEHEEK 300

Query: 301 SDSANLIPTQKFLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTI 360
           + S +L  +   L+KA     +G+T    W  CT  QVE VKI++ ++PIF CTI+ N  
Sbjct: 301 TPSQSLTNSLTCLNKAI----EGKTHHI-WLECTVQQVEDVKIVLKMLPIFGCTIMLNCC 360

Query: 361 LAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHT 420
           LAQL T+SV Q + MN ++  +F++P ASL   P + ++ + P YD   +PFARK T   
Sbjct: 361 LAQLSTYSVHQAATMNRKIV-NFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSE 420

Query: 421 SGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSA-----LNLNQT--VSIFWITPQFLIF 480
            GI+ L+RIG GL LS  +M  AA++E KR+  A     L+  +T  ++  WI  Q+L  
Sbjct: 421 IGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFL 480

Query: 481 GLSEMFTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGG 540
           G +++FT  GL+EFF+ ++   M++  T++++ S + G+YLSS++V +VN++T ++    
Sbjct: 481 GSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSP 540

Query: 541 WLHDNNLNKDRLDLFYWLLAILSFLNFLNYLFWS 568
           WL +  LN++RLDLFYWL+ +LS +NFL+YLFW+
Sbjct: 541 WLGE-KLNRNRLDLFYWLMCVLSVVNFLHYLFWA 555

BLAST of MC10g0487 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 2.9e-103
Identity = 218/558 (39.07%), Postives = 333/558 (59.68%), Query Frame = 0

Query: 21  TVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSANVV 80
           TVD    P+N    G  +A  F+LG +  E +A   +G NL+ Y  + ++   + +AN V
Sbjct: 12  TVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNV 71

Query: 81  TNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFILLSVQAHLPQLKPPQCNMLS 140
           TN+ GT ++  L+G F++D+YLG +WT+  F F+ +SG  LL++ A +P LKP  CN   
Sbjct: 72  TNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCN--- 131

Query: 141 EDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLSTYFN 200
              + C       A +FFVALY++ALG+G +KP + + GADQF  +N+     K S++FN
Sbjct: 132 --ADTCHPNSSQTA-VFFVALYMIALGTGGIKPCVSSFGADQFD-ENDENEKIKKSSFFN 191

Query: 201 AAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGPI 260
             YF+ +VG LIA T++VWIQ + G   GFGV    M + +     G  +YR + P G  
Sbjct: 192 WFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSP 251

Query: 261 FTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALSSDSANLIPTQ--KFLDKACI 320
            T I QV VAA  K     P +  +L  + ++  +       S  L+ T   KF DKA +
Sbjct: 252 LTRIFQVIVAAFRKISVKVPEDKSLLFETADDESN----IKGSRKLVHTDNLKFFDKAAV 311

Query: 321 KSQDGETKE---SPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQGSLM 380
           +SQ    K+   +PWRLC+ TQVE++K +I+++P++A  I+F T+ +Q+ T  V QG+ M
Sbjct: 312 ESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTM 371

Query: 381 NTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFGLFL 440
           +  + K+F IP ASL     + ++F  P+YD F +P ARKFT +  G + L+R+G GL +
Sbjct: 372 DQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVV 431

Query: 441 STFSMVSAAIMEKKRRDSALNLNQ------TVSIFWITPQFLIFGLSEMFTAVGLIEFFY 500
           S F+M++A ++E  R D     N        +SIFW  PQ+L+ G +E+FT +G +EFFY
Sbjct: 432 SIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFY 491

Query: 501 KQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRLDLFY 560
            Q+   M++  +A++  + + G YLS++LV++V KIT  +   GW+ D NLN+  LD F+
Sbjct: 492 DQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPD-NLNRGHLDYFF 551

Query: 561 WLLAILSFLNFLNYLFWS 568
           +LLA LSFLNFL YL+ S
Sbjct: 552 YLLATLSFLNFLVYLWIS 557

BLAST of MC10g0487 vs. NCBI nr
Match: XP_022136438.1 (protein NRT1/ PTR FAMILY 4.3-like [Momordica charantia])

HSP 1 Score: 1199 bits (3103), Expect = 0.0
Identity = 609/611 (99.67%), Postives = 609/611 (99.67%), Query Frame = 0

Query: 1   MEMEMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNN 60
           MEMEMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNN
Sbjct: 1   MEMEMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNN 60

Query: 61  LITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFI 120
           LITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFI
Sbjct: 61  LITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFI 120

Query: 121 LLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGA 180
           LLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGA
Sbjct: 121 LLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGA 180

Query: 181 DQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMG 240
           DQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMG
Sbjct: 181 DQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMG 240

Query: 241 LISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALS 300
           LISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALS
Sbjct: 241 LISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALS 300

Query: 301 SDSANLIPTQKF--LDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFN 360
           SDSANLIPTQKF  LDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFN
Sbjct: 301 SDSANLIPTQKFRFLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFN 360

Query: 361 TILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTG 420
           TILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTG
Sbjct: 361 TILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTG 420

Query: 421 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEM 480
           HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEM
Sbjct: 421 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEM 480

Query: 481 FTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDN 540
           FTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDN
Sbjct: 481 FTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDN 540

Query: 541 NLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFN 600
           NLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFN
Sbjct: 541 NLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFN 600

Query: 601 SSKTNIDEYVP 609
           SSKTNIDEYVP
Sbjct: 601 SSKTNIDEYVP 611

BLAST of MC10g0487 vs. NCBI nr
Match: XP_023552004.1 (protein NRT1/ PTR FAMILY 4.3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1066 bits (2757), Expect = 0.0
Identity = 543/616 (88.15%), Postives = 573/616 (93.02%), Query Frame = 0

Query: 3   MEMERRKDFKGDEDR-------DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIA 62
           MEMERR+DFKG+ +        + VTVDWRGRPSN +SHGGMRAAAFVLGLQAFEIMAIA
Sbjct: 1   MEMERRRDFKGENNANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMAIA 60

Query: 63  AVGNNLITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVE 122
           AVGNNLITY INEMHFSLSKSAN+VTNFVGTVFLLALVGGFLSDSYLGSFWTML+FGFVE
Sbjct: 61  AVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVE 120

Query: 123 LSGFILLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNM 182
           LSGFILLSVQAH+PQLKPPQCNML  DG  C EAKGLKALIFFVALYLVALGSGCVKPNM
Sbjct: 121 LSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKPNM 180

Query: 183 IAHGADQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAA 242
           IAHGADQFSPQ+ P  SK LSTYFNAAY AFS+GELIALTL+VWIQTHSGMDVGFGVSAA
Sbjct: 181 IAHGADQFSPQS-PSQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAA 240

Query: 243 VMAMGLISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDH 302
           VMA+GLISLV GIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQ + D 
Sbjct: 241 VMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND- 300

Query: 303 KVALSSDSANLIPTQKF--LDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFAC 362
           KV LSS SANLIPTQKF  L+KACIK+QD   KESPWRLCTP QVEQVKIL+SVVPIFAC
Sbjct: 301 KVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360

Query: 363 TIIFNTILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFA 422
           TI+FNTILAQLQTFSVQQGSLMNT+LTKSFHIPPASLQ+IPYIMLI IVPLYDTFFVPFA
Sbjct: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVPFA 420

Query: 423 RKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIF 482
           RKFTGHTSGISPLKRIGFGLFLS FSMV+AAIMEKKRRDSA+NLNQT+SIFWITPQFLIF
Sbjct: 421 RKFTGHTSGISPLKRIGFGLFLSAFSMVAAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480

Query: 483 GLSEMFTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGG 542
           GLSEMFTAVGLIEFFYKQS KGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKIT++SSNGG
Sbjct: 481 GLSEMFTAVGLIEFFYKQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540

Query: 543 WLHDNNLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYH 602
           WLHDNNLNKDRLDLFYWLLA LSFLNFLNYLFWS WFDEHPS+SQK  H++NPHHGEDYH
Sbjct: 541 WLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600

Query: 603 SYNFNSSKTNIDEYVP 609
            YNFNSSK N+D+YVP
Sbjct: 601 IYNFNSSKNNVDDYVP 614

BLAST of MC10g0487 vs. NCBI nr
Match: XP_022929221.1 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1064 bits (2752), Expect = 0.0
Identity = 543/618 (87.86%), Postives = 572/618 (92.56%), Query Frame = 0

Query: 3   MEMERRKDFKGDEDR---------DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMA 62
           MEMERR+DFKG+ +          + VTVDWRGRPSN +SHGGMRAAAFVLGLQAFEIMA
Sbjct: 1   MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA 60

Query: 63  IAAVGNNLITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGF 122
           IAAVGNNLITY INEMHFSLSKSAN+VTNFVGTVFLLALVGGFLSDSYLGSFWTML+FGF
Sbjct: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120

Query: 123 VELSGFILLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKP 182
           VELSGFILLSVQAH+PQLKPPQCNML  DG  C EAKGLKALIFFVALYLVALGSGCVKP
Sbjct: 121 VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180

Query: 183 NMIAHGADQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVS 242
           NMIAHGADQFSPQ+ P  SK LSTYFNAAY AFS+GELIALTL+VWIQTHSGMDVGFGVS
Sbjct: 181 NMIAHGADQFSPQS-PTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVS 240

Query: 243 AAVMAMGLISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNV 302
           AAVMA+GLISLV GIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQ + 
Sbjct: 241 AAVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSN 300

Query: 303 DHKVALSSDSANLIPTQKF--LDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIF 362
           D KV LSS SANLIPTQKF  L+KACIK+QD   KESPWRLCTP QVEQVKIL+SVVPIF
Sbjct: 301 D-KVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIF 360

Query: 363 ACTIIFNTILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVP 422
           ACTI+FNTILAQLQTFSVQQGSLMNT+LTKSFHIPPASLQ+IPYIMLI IVPLYDTFFVP
Sbjct: 361 ACTIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVP 420

Query: 423 FARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFL 482
           FARKFTGHTSGISPLKRIGFGLFLS FSMVSAAIMEKKRRDSA+NLNQT+SIFWITPQFL
Sbjct: 421 FARKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFL 480

Query: 483 IFGLSEMFTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSN 542
           IFGLSEMFTAVGLIEFFY QS KGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKIT++SSN
Sbjct: 481 IFGLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSN 540

Query: 543 GGWLHDNNLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGED 602
           GGWLHDNNLNKDRLDLFYWLLA LSFLNFLNYLFWS WFDEHPS+SQK  H++NPHHGED
Sbjct: 541 GGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGED 600

Query: 603 YHSYNFNSSKTNIDEYVP 609
           YH YNFNSSK N+D+YVP
Sbjct: 601 YHIYNFNSSKNNVDDYVP 616

BLAST of MC10g0487 vs. NCBI nr
Match: XP_022985329.1 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1060 bits (2742), Expect = 0.0
Identity = 541/616 (87.82%), Postives = 570/616 (92.53%), Query Frame = 0

Query: 3   MEMERRKDFKGDEDR-------DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIA 62
           MEMERR+DFKG+ +        + VTVDWRGRPSN +SHGGMRAAAFVLGLQAFEIMAIA
Sbjct: 1   MEMERRRDFKGENNANANAMPSEDVTVDWRGRPSNSVSHGGMRAAAFVLGLQAFEIMAIA 60

Query: 63  AVGNNLITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVE 122
           AVGNNLITY INEMHFSLSKSAN+VTNFVGTVFLLALVGGFLSDSYLGSFWTML+FGFVE
Sbjct: 61  AVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVE 120

Query: 123 LSGFILLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNM 182
           LSGFILLSVQAH+PQLKPPQCNML  DG  C EAKGLKALIFFVALYLVALGSGCVKPNM
Sbjct: 121 LSGFILLSVQAHVPQLKPPQCNMLKSDGNECVEAKGLKALIFFVALYLVALGSGCVKPNM 180

Query: 183 IAHGADQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAA 242
           IAHGADQFSPQ+ P  SK LSTYFNAAY AFS+GELIALTL+VWIQTHSGMDVGFGVSAA
Sbjct: 181 IAHGADQFSPQS-PSQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAA 240

Query: 243 VMAMGLISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDH 302
           VMA+GLISLV GIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQ + D 
Sbjct: 241 VMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND- 300

Query: 303 KVALSSDSANLIPTQKF--LDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFAC 362
           KV LSS SANLIPTQKF  L+KACIK+QD   KESPWRLCTP QVEQVKIL+SVVPIFAC
Sbjct: 301 KVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360

Query: 363 TIIFNTILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFA 422
           TI+FNTILAQLQTFSVQQGSLMNT+LTKS HIPPASLQ+IPYIMLI IVPLYDTFFVPFA
Sbjct: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSLHIPPASLQSIPYIMLILIVPLYDTFFVPFA 420

Query: 423 RKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIF 482
           RK TGHTSGISPLKRIGFGLFLS FSMVSAAIMEKKRRDSA+NLNQT+SIFWITPQFLIF
Sbjct: 421 RKITGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480

Query: 483 GLSEMFTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGG 542
           GLSEMFTAVGLIEFFYKQS KGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKIT++SSNGG
Sbjct: 481 GLSEMFTAVGLIEFFYKQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540

Query: 543 WLHDNNLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYH 602
           WLHDNNLNKDRLDLFYWLLA LSFLN LNYLFWS WFDEHPS+SQK  H++NPHHGEDYH
Sbjct: 541 WLHDNNLNKDRLDLFYWLLAALSFLNLLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600

Query: 603 SYNFNSSKTNIDEYVP 609
            YNFNSSK N+D+YVP
Sbjct: 601 IYNFNSSKNNVDDYVP 614

BLAST of MC10g0487 vs. NCBI nr
Match: KAG7015441.1 (Protein NRT1/ PTR FAMILY 4.3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1057 bits (2733), Expect = 0.0
Identity = 540/620 (87.10%), Postives = 569/620 (91.77%), Query Frame = 0

Query: 3   MEMERRKDFKGDEDR-----------DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEI 62
           MEMERR+DFKG+ +            + VTVDWRGRPSN +SHGGMRAAAFVLGLQAFEI
Sbjct: 1   MEMERRRDFKGENNANANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEI 60

Query: 63  MAIAAVGNNLITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVF 122
           MAIAAVGNNLITY INEMHFSLSKSAN+VTNFVGTVFLLAL GGFLSDSYLGSFWTML+F
Sbjct: 61  MAIAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALFGGFLSDSYLGSFWTMLIF 120

Query: 123 GFVELSGFILLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCV 182
           GFVELSGFILLSVQAH+PQLKPPQCNM   DG  C EAKGLKALIFFVALYLVALGSGCV
Sbjct: 121 GFVELSGFILLSVQAHVPQLKPPQCNMSESDGNECVEAKGLKALIFFVALYLVALGSGCV 180

Query: 183 KPNMIAHGADQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFG 242
           KPNMIAHGADQFSPQ+ P  SK LSTYFNAAY AFS+GELIALTL+VWIQTHSGMDVGFG
Sbjct: 181 KPNMIAHGADQFSPQS-PTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFG 240

Query: 243 VSAAVMAMGLISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQN 302
           VSAAVMA+GLISLV GIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQ 
Sbjct: 241 VSAAVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQT 300

Query: 303 NVDHKVALSSDSANLIPTQKF--LDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVP 362
           + D KV LSS SANLIPTQKF  L+KACIK+QD   KESPWRLCTP QVEQVKIL+SVVP
Sbjct: 301 SND-KVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVP 360

Query: 363 IFACTIIFNTILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFF 422
           IFACTI+FNTILAQLQTFSVQQGSLMNT+LTKSFHIPPASLQ+IPYIMLI IVPLYDTFF
Sbjct: 361 IFACTIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFF 420

Query: 423 VPFARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQ 482
           VPFARKFTGHTSGISPLKRIGFGLFLS FSMVSAAIMEKKRRDSA+NL QT+SIFWITPQ
Sbjct: 421 VPFARKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLTQTISIFWITPQ 480

Query: 483 FLIFGLSEMFTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATS 542
           FLIFGLSEMFTAVGLIEFFY QS KGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKIT++S
Sbjct: 481 FLIFGLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSS 540

Query: 543 SNGGWLHDNNLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHG 602
           SNGGWLHDNNLNKDRLDLFYWLLA LSFLNFLNYLFWS WFDEHPS+SQK  H++NPHHG
Sbjct: 541 SNGGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHG 600

Query: 603 EDYHSYNFNSSKTNIDEYVP 609
           EDYH YNFNSSK N+D+YVP
Sbjct: 601 EDYHIYNFNSSKNNVDDYVP 618

BLAST of MC10g0487 vs. ExPASy TrEMBL
Match: A0A6J1C7K1 (protein NRT1/ PTR FAMILY 4.3-like OS=Momordica charantia OX=3673 GN=LOC111008146 PE=3 SV=1)

HSP 1 Score: 1199 bits (3103), Expect = 0.0
Identity = 609/611 (99.67%), Postives = 609/611 (99.67%), Query Frame = 0

Query: 1   MEMEMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNN 60
           MEMEMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNN
Sbjct: 1   MEMEMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNN 60

Query: 61  LITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFI 120
           LITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFI
Sbjct: 61  LITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFI 120

Query: 121 LLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGA 180
           LLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGA
Sbjct: 121 LLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGA 180

Query: 181 DQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMG 240
           DQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMG
Sbjct: 181 DQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMG 240

Query: 241 LISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALS 300
           LISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALS
Sbjct: 241 LISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALS 300

Query: 301 SDSANLIPTQKF--LDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFN 360
           SDSANLIPTQKF  LDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFN
Sbjct: 301 SDSANLIPTQKFRFLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFN 360

Query: 361 TILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTG 420
           TILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTG
Sbjct: 361 TILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTG 420

Query: 421 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEM 480
           HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEM
Sbjct: 421 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEM 480

Query: 481 FTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDN 540
           FTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDN
Sbjct: 481 FTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDN 540

Query: 541 NLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFN 600
           NLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFN
Sbjct: 541 NLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFN 600

Query: 601 SSKTNIDEYVP 609
           SSKTNIDEYVP
Sbjct: 601 SSKTNIDEYVP 611

BLAST of MC10g0487 vs. ExPASy TrEMBL
Match: A0A6J1EMI3 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435878 PE=3 SV=1)

HSP 1 Score: 1064 bits (2752), Expect = 0.0
Identity = 543/618 (87.86%), Postives = 572/618 (92.56%), Query Frame = 0

Query: 3   MEMERRKDFKGDEDR---------DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMA 62
           MEMERR+DFKG+ +          + VTVDWRGRPSN +SHGGMRAAAFVLGLQAFEIMA
Sbjct: 1   MEMERRRDFKGENNANANANAMPSEDVTVDWRGRPSNAVSHGGMRAAAFVLGLQAFEIMA 60

Query: 63  IAAVGNNLITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGF 122
           IAAVGNNLITY INEMHFSLSKSAN+VTNFVGTVFLLALVGGFLSDSYLGSFWTML+FGF
Sbjct: 61  IAAVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGF 120

Query: 123 VELSGFILLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKP 182
           VELSGFILLSVQAH+PQLKPPQCNML  DG  C EAKGLKALIFFVALYLVALGSGCVKP
Sbjct: 121 VELSGFILLSVQAHVPQLKPPQCNMLESDGNECVEAKGLKALIFFVALYLVALGSGCVKP 180

Query: 183 NMIAHGADQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVS 242
           NMIAHGADQFSPQ+ P  SK LSTYFNAAY AFS+GELIALTL+VWIQTHSGMDVGFGVS
Sbjct: 181 NMIAHGADQFSPQS-PTQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVS 240

Query: 243 AAVMAMGLISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNV 302
           AAVMA+GLISLV GIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQ + 
Sbjct: 241 AAVMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSN 300

Query: 303 DHKVALSSDSANLIPTQKF--LDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIF 362
           D KV LSS SANLIPTQKF  L+KACIK+QD   KESPWRLCTP QVEQVKIL+SVVPIF
Sbjct: 301 D-KVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIF 360

Query: 363 ACTIIFNTILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVP 422
           ACTI+FNTILAQLQTFSVQQGSLMNT+LTKSFHIPPASLQ+IPYIMLI IVPLYDTFFVP
Sbjct: 361 ACTIVFNTILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIIIVPLYDTFFVP 420

Query: 423 FARKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFL 482
           FARKFTGHTSGISPLKRIGFGLFLS FSMVSAAIMEKKRRDSA+NLNQT+SIFWITPQFL
Sbjct: 421 FARKFTGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFL 480

Query: 483 IFGLSEMFTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSN 542
           IFGLSEMFTAVGLIEFFY QS KGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKIT++SSN
Sbjct: 481 IFGLSEMFTAVGLIEFFYNQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSN 540

Query: 543 GGWLHDNNLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGED 602
           GGWLHDNNLNKDRLDLFYWLLA LSFLNFLNYLFWS WFDEHPS+SQK  H++NPHHGED
Sbjct: 541 GGWLHDNNLNKDRLDLFYWLLAALSFLNFLNYLFWSKWFDEHPSVSQKPQHNDNPHHGED 600

Query: 603 YHSYNFNSSKTNIDEYVP 609
           YH YNFNSSK N+D+YVP
Sbjct: 601 YHIYNFNSSKNNVDDYVP 616

BLAST of MC10g0487 vs. ExPASy TrEMBL
Match: A0A6J1JCZ7 (protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483371 PE=3 SV=1)

HSP 1 Score: 1060 bits (2742), Expect = 0.0
Identity = 541/616 (87.82%), Postives = 570/616 (92.53%), Query Frame = 0

Query: 3   MEMERRKDFKGDEDR-------DHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIA 62
           MEMERR+DFKG+ +        + VTVDWRGRPSN +SHGGMRAAAFVLGLQAFEIMAIA
Sbjct: 1   MEMERRRDFKGENNANANAMPSEDVTVDWRGRPSNSVSHGGMRAAAFVLGLQAFEIMAIA 60

Query: 63  AVGNNLITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVE 122
           AVGNNLITY INEMHFSLSKSAN+VTNFVGTVFLLALVGGFLSDSYLGSFWTML+FGFVE
Sbjct: 61  AVGNNLITYLINEMHFSLSKSANIVTNFVGTVFLLALVGGFLSDSYLGSFWTMLIFGFVE 120

Query: 123 LSGFILLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNM 182
           LSGFILLSVQAH+PQLKPPQCNML  DG  C EAKGLKALIFFVALYLVALGSGCVKPNM
Sbjct: 121 LSGFILLSVQAHVPQLKPPQCNMLKSDGNECVEAKGLKALIFFVALYLVALGSGCVKPNM 180

Query: 183 IAHGADQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAA 242
           IAHGADQFSPQ+ P  SK LSTYFNAAY AFS+GELIALTL+VWIQTHSGMDVGFGVSAA
Sbjct: 181 IAHGADQFSPQS-PSQSKTLSTYFNAAYLAFSIGELIALTLLVWIQTHSGMDVGFGVSAA 240

Query: 243 VMAMGLISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDH 302
           VMA+GLISLV GIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQ + D 
Sbjct: 241 VMALGLISLVAGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQTSND- 300

Query: 303 KVALSSDSANLIPTQKF--LDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFAC 362
           KV LSS SANLIPTQKF  L+KACIK+QD   KESPWRLCTP QVEQVKIL+SVVPIFAC
Sbjct: 301 KVGLSSHSANLIPTQKFRFLNKACIKNQDEAAKESPWRLCTPNQVEQVKILVSVVPIFAC 360

Query: 363 TIIFNTILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFA 422
           TI+FNTILAQLQTFSVQQGSLMNT+LTKS HIPPASLQ+IPYIMLI IVPLYDTFFVPFA
Sbjct: 361 TIVFNTILAQLQTFSVQQGSLMNTQLTKSLHIPPASLQSIPYIMLILIVPLYDTFFVPFA 420

Query: 423 RKFTGHTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIF 482
           RK TGHTSGISPLKRIGFGLFLS FSMVSAAIMEKKRRDSA+NLNQT+SIFWITPQFLIF
Sbjct: 421 RKITGHTSGISPLKRIGFGLFLSAFSMVSAAIMEKKRRDSAVNLNQTISIFWITPQFLIF 480

Query: 483 GLSEMFTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGG 542
           GLSEMFTAVGLIEFFYKQS KGMQAFLTA+TYCSYSFGFYLSSLLVSMVNKIT++SSNGG
Sbjct: 481 GLSEMFTAVGLIEFFYKQSIKGMQAFLTAMTYCSYSFGFYLSSLLVSMVNKITSSSSNGG 540

Query: 543 WLHDNNLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYH 602
           WLHDNNLNKDRLDLFYWLLA LSFLN LNYLFWS WFDEHPS+SQK  H++NPHHGEDYH
Sbjct: 541 WLHDNNLNKDRLDLFYWLLAALSFLNLLNYLFWSKWFDEHPSVSQKPQHNDNPHHGEDYH 600

Query: 603 SYNFNSSKTNIDEYVP 609
            YNFNSSK N+D+YVP
Sbjct: 601 IYNFNSSKNNVDDYVP 614

BLAST of MC10g0487 vs. ExPASy TrEMBL
Match: A0A6J1FR74 (protein NRT1/ PTR FAMILY 4.3-like OS=Cucurbita moschata OX=3662 GN=LOC111447459 PE=3 SV=1)

HSP 1 Score: 1019 bits (2634), Expect = 0.0
Identity = 529/595 (88.91%), Postives = 561/595 (94.29%), Query Frame = 0

Query: 1   MEMEMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNN 60
           M+M++ERR+D      +D +TVDWRGRPSNP++HGGMRAAAFVLGLQ+FEIMAIAAVGNN
Sbjct: 7   MDMDIERRRDTHLMPSQD-LTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNN 66

Query: 61  LITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFI 120
           LITY INEMHFSLSKSANVVTNFVGTVFLLAL+GGFLSDSYLGSFWTML+FGFVELSGFI
Sbjct: 67  LITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFI 126

Query: 121 LLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGA 180
           LLSVQAHLPQLKPPQCNMLSE G++C EAKG+KALIFFVALYLVALGSGCVKPNMIAHGA
Sbjct: 127 LLSVQAHLPQLKPPQCNMLSE-GDHCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGA 186

Query: 181 DQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMG 240
           DQF+PQ+ P  SK+LSTYFNAAYFAFS+GELIALTLVVWIQTHSGMDVGFGVSAAVMAMG
Sbjct: 187 DQFTPQD-PSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMG 246

Query: 241 LISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALS 300
           LISLVGGIFYYRNKPPQG IF PILQVFVAA+LKRKQVCPSNP MLHGSQ + +HKVALS
Sbjct: 247 LISLVGGIFYYRNKPPQGAIFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTS-NHKVALS 306

Query: 301 SDSANLIPTQKF--LDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFN 360
           S SANLIPTQKF  LDKACIKSQDG T+ESPWRLCT TQVEQVKILISVVPIFACTIIFN
Sbjct: 307 SHSANLIPTQKFRFLDKACIKSQDGTTRESPWRLCTLTQVEQVKILISVVPIFACTIIFN 366

Query: 361 TILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTG 420
           TILAQLQTFSVQQGSLMNT+LTKSFHIPPASLQ+IPYIMLIFIVPLYDTFFVPFARKFTG
Sbjct: 367 TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG 426

Query: 421 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEM 480
           HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSA+N NQT+SIFWITPQFLIFGLSEM
Sbjct: 427 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM 486

Query: 481 FTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDN 540
           FTAVGLIEFFYKQS KGMQAFLTAITYCSYSFGFYLSSLLVSMVNKIT++S+NGGWLHDN
Sbjct: 487 FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN 546

Query: 541 NLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYH 593
           NLNKDRLDLFYWLLA LSFLNFLNYLFWS+WF + PSLSQK   D N HHGE+ H
Sbjct: 547 NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPSLSQKLQQD-NLHHGENSH 596

BLAST of MC10g0487 vs. ExPASy TrEMBL
Match: A0A6J1JRW4 (protein NRT1/ PTR FAMILY 4.3-like OS=Cucurbita maxima OX=3661 GN=LOC111489235 PE=3 SV=1)

HSP 1 Score: 1018 bits (2633), Expect = 0.0
Identity = 527/594 (88.72%), Postives = 557/594 (93.77%), Query Frame = 0

Query: 1   MEMEMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNN 60
           MEM++ERR+D       D +TVDWRGRPSNP++HGGMRAAAFVLGLQ+FEIMAIAAVGNN
Sbjct: 5   MEMDIERRRDTHLMPSED-LTVDWRGRPSNPVAHGGMRAAAFVLGLQSFEIMAIAAVGNN 64

Query: 61  LITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFI 120
           LITY INEMHFSLSKSANVVTNFVGTVFLLAL+GGFLSDSYLGSFWTML+FGFVELSGFI
Sbjct: 65  LITYVINEMHFSLSKSANVVTNFVGTVFLLALLGGFLSDSYLGSFWTMLIFGFVELSGFI 124

Query: 121 LLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGA 180
           LLSVQAHLPQLKPPQCNMLSE G++C EAKG+KALIFFVALYLVALGSGCVKPNMIAHGA
Sbjct: 125 LLSVQAHLPQLKPPQCNMLSE-GDDCVEAKGIKALIFFVALYLVALGSGCVKPNMIAHGA 184

Query: 181 DQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMG 240
           DQF+PQ+ P  SK+LSTYFNAAYFAFS+GELIALTLVVWIQTHSGMDVGFGVSAAVMAMG
Sbjct: 185 DQFTPQD-PSQSKRLSTYFNAAYFAFSIGELIALTLVVWIQTHSGMDVGFGVSAAVMAMG 244

Query: 241 LISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALS 300
           LISLVGGIFYYRNKPPQG +F PILQVFVAA+LKRKQVCPSNP MLHGSQ + +HKVALS
Sbjct: 245 LISLVGGIFYYRNKPPQGALFIPILQVFVAAVLKRKQVCPSNPQMLHGSQTS-NHKVALS 304

Query: 301 SDSANLIPTQKF--LDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFN 360
           S SANLIPTQKF  LDKACIKS DG T+ESPWRLCTPTQVEQVKILISVVPIFACTIIFN
Sbjct: 305 SHSANLIPTQKFRFLDKACIKSLDGTTRESPWRLCTPTQVEQVKILISVVPIFACTIIFN 364

Query: 361 TILAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTG 420
           TILAQLQTFSVQQGSLMNT+LTKSFHIPPASLQ+IPYIMLIFIVPLYDTFFVPFARKFTG
Sbjct: 365 TILAQLQTFSVQQGSLMNTQLTKSFHIPPASLQSIPYIMLIFIVPLYDTFFVPFARKFTG 424

Query: 421 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEM 480
           HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSA+N NQT+SIFWITPQFLIFGLSEM
Sbjct: 425 HTSGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSAVNSNQTISIFWITPQFLIFGLSEM 484

Query: 481 FTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDN 540
           FTAVGLIEFFYKQS KGMQAFLTAITYCSYSFGFYLSSLLVSMVNKIT++S+NGGWLHDN
Sbjct: 485 FTAVGLIEFFYKQSVKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITSSSTNGGWLHDN 544

Query: 541 NLNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQ-----HHDENPH 587
           NLNKDRLDLFYWLLA LSFLNFLNYLFWS+WF + P LSQK      HH ENPH
Sbjct: 545 NLNKDRLDLFYWLLAALSFLNFLNYLFWSSWFQDPPPLSQKLQQDNLHHGENPH 594

BLAST of MC10g0487 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 825.1 bits (2130), Expect = 3.7e-239
Identity = 425/570 (74.56%), Postives = 484/570 (84.91%), Query Frame = 0

Query: 4   EMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLIT 63
           E E   +    E  +  +VDWRGRPSNP  HGGMRAA FVLGLQAFEIM IAAVGNNLIT
Sbjct: 12  EQEEVSNENNWELAEEESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLIT 71

Query: 64  YAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFILLS 123
           Y INEMHF LSK+AN+VTNFVGT+F+ AL+GG+LSD++LGSFWT+++FGFVELSGFILLS
Sbjct: 72  YVINEMHFPLSKAANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLS 131

Query: 124 VQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQF 183
           VQAHLPQLKPP+CN L +  + CEEAKG KA+IFF+ALYLVALGSGCVKPNMIAHGADQF
Sbjct: 132 VQAHLPQLKPPKCNPLID--QTCEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQF 191

Query: 184 SPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLIS 243
           S Q++PK SK+LS+YFNAAYFAFS+GELIALTL+VW+QTHSGMD+GFGVSAA M MG+IS
Sbjct: 192 S-QSHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIIS 251

Query: 244 LVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVA---LS 303
           LV G  Y+RNK P+  IFTPI  V VAA+LKRK   PS+P MLHG     DH VA   + 
Sbjct: 252 LVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKLASPSDPRMLHG-----DHHVANDVVP 311

Query: 304 SDSANLIPTQKFLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTI 363
           S +    P  +FLDKACIK QD  TKESPWRLCT TQVEQVK LIS+VPIFA TI+FNTI
Sbjct: 312 SSTLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIVFNTI 371

Query: 364 LAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHT 423
           LAQLQTFSVQQGS MNT L+ SFHIPPASLQAIPYIMLIF+VPLYD+F VPFARK TGH 
Sbjct: 372 LAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLVPFARKLTGHN 431

Query: 424 SGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEMFT 483
           SGI PL RIG GLFLSTFSMVSAA++EKKRRDS++   + +SIFWITPQFLIFG+SEMFT
Sbjct: 432 SGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGRILSIFWITPQFLIFGISEMFT 491

Query: 484 AVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATS-SNGGWLHDNN 543
           AVGLIEFFYKQS+KGM++FL A+TYCSYSFGFY SS+LVS+VNKIT+TS  + GWL +N+
Sbjct: 492 AVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVDSKGWLGEND 551

Query: 544 LNKDRLDLFYWLLAILSFLNFLNYLFWSTW 570
           LNKDRLDLFYWLLA+LS LNFL+YLFWS W
Sbjct: 552 LNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

BLAST of MC10g0487 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 771.5 bits (1991), Expect = 4.9e-223
Identity = 412/603 (68.33%), Postives = 481/603 (79.77%), Query Frame = 0

Query: 4   EMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLIT 63
           E +R      +E  D + VD+RGRP  P  HGG RAA FVLG QAFE+MAIAAVGNNLIT
Sbjct: 9   EAKRGVIHTSEESLDDLCVDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLIT 68

Query: 64  YAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFILLS 123
           Y  NEMHF LSKSAN+VTNF+GTVFLL+L+GGFLSDSYLGSF TMLVFG +E+SGFILLS
Sbjct: 69  YVFNEMHFPLSKSANLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLS 128

Query: 124 VQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQF 183
           VQAHLP+L+PP+CNM S    +C EA G KA   + AL LVALGSGC+KPN+I+HGA+QF
Sbjct: 129 VQAHLPELRPPECNMKSTT-IHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQF 188

Query: 184 SPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLIS 243
                 K  +KLS++FNAAYFAFS+G+LIALTL+VW+QTHSGMDVGFGVSAAVMA G+IS
Sbjct: 189 ----QRKDLRKLSSFFNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMIS 248

Query: 244 LVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLH-GSQNNVDHKVALSSD 303
           LV G  +YRNKPP G IFTPI QVFVAA+ KRKQ+CPSNP+M+H  S + V  K  L S+
Sbjct: 249 LVAGTSFYRNKPPSGSIFTPIAQVFVAAITKRKQICPSNPNMVHQPSTDLVRVKPLLHSN 308

Query: 304 SANLIPTQKFLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILA 363
                   +FLDKACIK+Q G+  ESPWRLCT  QV QVKIL+SV+PIFACTIIFNTILA
Sbjct: 309 KF------RFLDKACIKTQ-GKAMESPWRLCTIEQVHQVKILLSVIPIFACTIIFNTILA 368

Query: 364 QLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSG 423
           QLQTFSVQQGS MNT +TK+F IPPASLQAIPYI+LIF VPLY+TFFVP ARK TG+ SG
Sbjct: 369 QLQTFSVQQGSSMNTHITKTFQIPPASLQAIPYIILIFFVPLYETFFVPLARKLTGNDSG 428

Query: 424 ISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSALNLNQTVSIFWITPQFLIFGLSEMFTAV 483
           ISPL+RIG GLFL+TFSMV+AA++EKKRR+S L  N  +SIFWI PQFLIFGLSEMFTAV
Sbjct: 429 ISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQNVMLSIFWIAPQFLIFGLSEMFTAV 488

Query: 484 GLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNG---GWLHDNN 543
           GL+EFFYKQSS+ MQ+FLTA+TYCSYSFGFYLSS+LVS VN++T+++ +G   GWL DN+
Sbjct: 489 GLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGSGTKEGWLGDND 548

Query: 544 LNKDRLDLFYWLLAILSFLNFLNYLFWSTWFDEHPSLSQKQHHDENPHHGEDYHSYNFNS 603
           LNKDRLD FYWLLA LSF+NF NYLFWS W+   PS +   HH    +  E   +     
Sbjct: 549 LNKDRLDHFYWLLASLSFINFFNYLFWSRWYSCDPSAT---HHSAEVNSLEALENGEIKD 596

BLAST of MC10g0487 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 412.9 bits (1060), Expect = 4.4e-115
Identity = 240/568 (42.25%), Postives = 346/568 (60.92%), Query Frame = 0

Query: 23  DWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFSLSKSANVVTN 82
           DWR R +    HGGM AA+FVL ++  E +A  A  +NL+ Y    MH S SKSAN VTN
Sbjct: 15  DWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTN 74

Query: 83  FVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFILLSVQAHLPQLKPPQCNMLSED 142
           F+GT FLLAL+GGFLSD++  +F   L+   +E  G I+L++QA  P L PP C     D
Sbjct: 75  FMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSC-----D 134

Query: 143 GENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHSKKLSTYFNAA 202
              CEE  G KA + FV LYLVALG G +K ++ +HGA+QF  ++ PK  K+ ST+FN  
Sbjct: 135 SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFD-ESTPKGRKQRSTFFNYF 194

Query: 203 YFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYRNKPPQGPIFT 262
            F  + G L+A+T VVW++ + G + GFGVS   + + ++  + G  +YRNK P G   T
Sbjct: 195 VFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLT 254

Query: 263 PILQVFVAALLK------------RKQVCPSNPHMLHGSQNNVDHKVALSSD-SANLIPT 322
            IL+V +AA +K               V PSN H +   +  V+ +  L        +P 
Sbjct: 255 TILKVLLAASVKCCSSGSSSNAVASMSVSPSN-HCVSKGKKEVESQGELEKPRQEEALPP 314

Query: 323 QKFLDKACIKSQDGETKESP-WRL--CTPTQVEQVKILISVVPIFACTIIFNTILAQLQT 382
           +  L  + +K  +G   E P  RL  CT  QVE VKI++ ++PIFACTI+ N  LAQL T
Sbjct: 315 RAQLTNS-LKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLST 374

Query: 383 FSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPL 442
           FSVQQ + MNT++  S  IPPASL   P + ++ + P+YD   +PFARK T   +G++ L
Sbjct: 375 FSVQQAASMNTKI-GSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHL 434

Query: 443 KRIGFGLFLSTFSMVSAAIMEKKRRDSA-----LNLNQT--VSIFWITPQFLIFGLSEMF 502
           +RIG GL LS  +M  AA++E KR+  A     L+  +T  V+  WI  Q+L  G +++F
Sbjct: 435 QRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLF 494

Query: 503 TAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNN 562
           T  GL+E+F+ ++   M++  T++++ S + G+YLSS++VS+VN IT +S N  WL   +
Sbjct: 495 TLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGSSGNTPWLRGKS 554

Query: 563 LNKDRLDLFYWLLAILSFLNFLNYLFWS 568
           +N+ +LD FYWL+ +LS  NFL+YLFW+
Sbjct: 555 INRYKLDYFYWLMCVLSAANFLHYLFWA 573

BLAST of MC10g0487 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 395.2 bits (1014), Expect = 9.5e-110
Identity = 229/574 (39.90%), Postives = 344/574 (59.93%), Query Frame = 0

Query: 1   MEMEMERRKDFKGDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNN 60
           ME+EM       GD  +     DWR + +    HGGM AA+FVL ++  E +A  A  +N
Sbjct: 1   MEVEMH------GDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASN 60

Query: 61  LITYAINEMHFSLSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFI 120
           L+ Y  N MH SL++S++ VT F+ T FLLAL+GGFL+D++  +F   L+   +E  G I
Sbjct: 61  LVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLI 120

Query: 121 LLSVQAHLPQLKPPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGA 180
           LL++QA  P L PP C   S     CE   G KA   FV LYLV+LG G +K ++ +HGA
Sbjct: 121 LLTIQARRPSLMPPPCK--SSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGA 180

Query: 181 DQFSPQNNPKHSKKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMG 240
           +QF  +  PK  K+ ST+FN   F  S G L+A+T VVWI+ + G + GFGVS   + + 
Sbjct: 181 EQFD-EGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLS 240

Query: 241 LISLVGGIFYYRNKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALS 300
           ++  + G  +Y+NK P+G   T I +V +AA +     C S     H +   V  +    
Sbjct: 241 ILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVS---CSSKTSSNHFTSREVQSEHEEK 300

Query: 301 SDSANLIPTQKFLDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTI 360
           + S +L  +   L+KA     +G+T    W  CT  QVE VKI++ ++PIF CTI+ N  
Sbjct: 301 TPSQSLTNSLTCLNKAI----EGKTHHI-WLECTVQQVEDVKIVLKMLPIFGCTIMLNCC 360

Query: 361 LAQLQTFSVQQGSLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHT 420
           LAQL T+SV Q + MN ++  +F++P ASL   P + ++ + P YD   +PFARK T   
Sbjct: 361 LAQLSTYSVHQAATMNRKIV-NFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSE 420

Query: 421 SGISPLKRIGFGLFLSTFSMVSAAIMEKKRRDSA-----LNLNQT--VSIFWITPQFLIF 480
            GI+ L+RIG GL LS  +M  AA++E KR+  A     L+  +T  ++  WI  Q+L  
Sbjct: 421 IGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFL 480

Query: 481 GLSEMFTAVGLIEFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGG 540
           G +++FT  GL+EFF+ ++   M++  T++++ S + G+YLSS++V +VN++T ++    
Sbjct: 481 GSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSP 540

Query: 541 WLHDNNLNKDRLDLFYWLLAILSFLNFLNYLFWS 568
           WL +  LN++RLDLFYWL+ +LS +NFL+YLFW+
Sbjct: 541 WLGE-KLNRNRLDLFYWLMCVLSVVNFLHYLFWA 555

BLAST of MC10g0487 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 394.4 bits (1012), Expect = 1.6e-109
Identity = 225/562 (40.04%), Postives = 339/562 (60.32%), Query Frame = 0

Query: 13  GDEDRDHVTVDWRGRPSNPLSHGGMRAAAFVLGLQAFEIMAIAAVGNNLITYAINEMHFS 72
           GD  +     DWR + +    HGGM AA+FVL ++  E +A  A  +NL+ Y  N MH S
Sbjct: 3   GDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMS 62

Query: 73  LSKSANVVTNFVGTVFLLALVGGFLSDSYLGSFWTMLVFGFVELSGFILLSVQAHLPQLK 132
           L++S++ VT F+ T FLLAL+GGFL+D++  +F   L+   +E  G ILL++QA  P L 
Sbjct: 63  LARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLM 122

Query: 133 PPQCNMLSEDGENCEEAKGLKALIFFVALYLVALGSGCVKPNMIAHGADQFSPQNNPKHS 192
           PP C   S     CE   G KA   FV LYLV+LG G +K ++ +HGA+QF  +  PK  
Sbjct: 123 PPPCK--SSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFD-EGTPKGR 182

Query: 193 KKLSTYFNAAYFAFSVGELIALTLVVWIQTHSGMDVGFGVSAAVMAMGLISLVGGIFYYR 252
           K+ ST+FN   F  S G L+A+T VVWI+ + G + GFGVS   + + ++  + G  +Y+
Sbjct: 183 KQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYK 242

Query: 253 NKPPQGPIFTPILQVFVAALLKRKQVCPSNPHMLHGSQNNVDHKVALSSDSANLIPTQKF 312
           NK P+G   T I +V +AA +     C S     H +   V  +    + S +L  +   
Sbjct: 243 NKIPRGSPLTTIFKVLLAASIVS---CSSKTSSNHFTSREVQSEHEEKTPSQSLTNSLTC 302

Query: 313 LDKACIKSQDGETKESPWRLCTPTQVEQVKILISVVPIFACTIIFNTILAQLQTFSVQQG 372
           L+KA     +G+T    W  CT  QVE VKI++ ++PIF CTI+ N  LAQL T+SV Q 
Sbjct: 303 LNKAI----EGKTHHI-WLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQA 362

Query: 373 SLMNTELTKSFHIPPASLQAIPYIMLIFIVPLYDTFFVPFARKFTGHTSGISPLKRIGFG 432
           + MN ++  +F++P ASL   P + ++ + P YD   +PFARK T    GI+ L+RIG G
Sbjct: 363 ATMNRKIV-NFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVG 422

Query: 433 LFLSTFSMVSAAIMEKKRRDSA-----LNLNQT--VSIFWITPQFLIFGLSEMFTAVGLI 492
           L LS  +M  AA++E KR+  A     L+  +T  ++  WI  Q+L  G +++FT  GL+
Sbjct: 423 LVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLL 482

Query: 493 EFFYKQSSKGMQAFLTAITYCSYSFGFYLSSLLVSMVNKITATSSNGGWLHDNNLNKDRL 552
           EFF+ ++   M++  T++++ S + G+YLSS++V +VN++T ++    WL +  LN++RL
Sbjct: 483 EFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWLGE-KLNRNRL 542

Query: 553 DLFYWLLAILSFLNFLNYLFWS 568
           DLFYWL+ +LS +NFL+YLFW+
Sbjct: 543 DLFYWLMCVLSVVNFLHYLFWA 551

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93VV55.2e-23874.56Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
Q56XQ66.9e-22268.33Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1[more]
Q8H1576.2e-11442.25Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q8VYE41.3e-10839.90Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q9M3902.9e-10339.07Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022136438.10.099.67protein NRT1/ PTR FAMILY 4.3-like [Momordica charantia][more]
XP_023552004.10.088.15protein NRT1/ PTR FAMILY 4.3-like [Cucurbita pepo subsp. pepo][more]
XP_022929221.10.087.86protein NRT1/ PTR FAMILY 4.3-like isoform X1 [Cucurbita moschata][more]
XP_022985329.10.087.82protein NRT1/ PTR FAMILY 4.3-like isoform X1 [Cucurbita maxima][more]
KAG7015441.10.087.10Protein NRT1/ PTR FAMILY 4.3, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
Match NameE-valueIdentityDescription
A0A6J1C7K10.099.67protein NRT1/ PTR FAMILY 4.3-like OS=Momordica charantia OX=3673 GN=LOC111008146... [more]
A0A6J1EMI30.087.86protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1JCZ70.087.82protein NRT1/ PTR FAMILY 4.3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1FR740.088.91protein NRT1/ PTR FAMILY 4.3-like OS=Cucurbita moschata OX=3662 GN=LOC111447459 ... [more]
A0A6J1JRW40.088.72protein NRT1/ PTR FAMILY 4.3-like OS=Cucurbita maxima OX=3661 GN=LOC111489235 PE... [more]
Match NameE-valueIdentityDescription
AT1G59740.13.7e-23974.56Major facilitator superfamily protein [more]
AT1G33440.14.9e-22368.33Major facilitator superfamily protein [more]
AT1G69850.14.4e-11542.25nitrate transporter 1:2 [more]
AT1G27040.19.5e-11039.90Major facilitator superfamily protein [more]
AT1G27040.21.6e-10940.04Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 105..533
e-value: 1.7E-89
score: 300.4
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 18..582
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 10..576
e-value: 7.4E-163
score: 544.5
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 25..568
NoneNo IPR availablePANTHERPTHR11654:SF551PROTEIN NRT1/ PTR FAMILY 4.3coord: 18..582
NoneNo IPR availableCDDcd17414MFS_NPF4coord: 37..569
e-value: 0.0
score: 682.213

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0487.1MC10g0487.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006817 phosphate ion transport
biological_process GO:0050896 response to stimulus
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity