MC10g0465 (gene) Bitter gourd (Dali-11) v1

BLAST of MC10g0465 vs. ExPASy Swiss-Prot
Match: Q93YR2 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS1 PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.5e-55
Identity = 140/350 (40.00%), Postives = 202/350 (57.71%), Query Frame = 0

Query: 56  PLQKPSGFDNFL----STAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIM 115
           P +K   F  ++       ++ +P++V+ G ++  ++PS+F+W     P    + L + M
Sbjct: 79  PTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFNWVT---PNWTIVGLTITM 138

Query: 116 LAMGLTLELENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGC 175
           L MG+TL L++L    +  P  +  G + QY++MP +A  + K L LPP  + GLIL+GC
Sbjct: 139 LGMGMTLTLDDLRGA-LSMPKELFAGFLLQYSVMPLSAFFVSKLLNLPPHYAAGLILVGC 198

Query: 176 CPGGTASNVVTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTL 235
           CPGGTASN+VT IA+G+V LS++MT  +T+ AVI+TP LT  LA  Y+ +DA  L +STL
Sbjct: 199 CPGGTASNIVTYIARGNVALSVLMTAASTVSAVIMTPLLTAKLAKQYITVDALGLLMSTL 258

Query: 236 QVVVAPILFGSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATL 295
           QVV+ P+L G++L + F  LVK V P  P +AV T ++L      +N         SA L
Sbjct: 259 QVVLLPVLAGAFLNQYFKKLVKFVSPVMPPIAVGTVAILCGYAIGQN--------ASAIL 318

Query: 296 ASDASPWIVLKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQ 355
            S                  V+L+  LLH +GF  GY+ + I G      R ISIEVGMQ
Sbjct: 319 MSGKQ---------------VVLASCLLHISGFLFGYLFSRILGIDVASSRTISIEVGMQ 378

Query: 356 NSSLGVVLATSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKEE 402
           NS LGVVLAT HF + + A+P A+S+V  +I+GS L   WR   P   E+
Sbjct: 379 NSVLGVVLATQHFGNPLTAVPCAVSSVCHSILGSVLAGIWRRSAPKQLED 401

BLAST of MC10g0465 vs. ExPASy Swiss-Prot
Match: Q5VRB2 (Probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS2 PE=2 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.8e-53
Identity = 129/330 (39.09%), Postives = 191/330 (57.88%), Query Frame = 0

Query: 63  FDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLELEN 122
           ++  +    +L+P++V  G I+   KPS  +W        +++ LG +ML+MGLTL  E+
Sbjct: 103 YEKIVELLTTLFPVWVILGTIIGIYKPSMVTWLETD---LFTVGLGFLMLSMGLTLTFED 162

Query: 123 LFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNVVT 182
            F   M+ P ++  G +AQY I P     I   L L   L+ GLIL+ CCPGG ASNV T
Sbjct: 163 -FRRCMRNPWTVGVGFLAQYLIKPMLGFAIAMTLKLSAPLATGLILVSCCPGGQASNVAT 222

Query: 183 LIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILFGS 242
            I++G+V LS++MT C+T+GA+++TP LTK LAG  VP+DAA L++ST QVV+ P + G 
Sbjct: 223 YISKGNVALSVLMTTCSTIGAIVMTPLLTKLLAGQLVPVDAAGLAISTFQVVLLPTIVGV 282

Query: 243 YLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIVLK 302
              + FP   + +I   PL+ VL ++LL                        ASP   + 
Sbjct: 283 LAHEYFPKFTERIISITPLIGVLLTTLLC-----------------------ASPIGQVS 342

Query: 303 NIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLATS 362
            ++  + G +I+ V LLH A F +GY  + +  + E   R ISIE GMQ+S+LG +LA  
Sbjct: 343 EVLKAQGGQLIIPVALLHVAAFALGYWLSKVSSFGESTSRTISIECGMQSSALGFLLAQK 402

Query: 363 HFSSTMVALPPAMSAVIMNIMGSSLGFFWR 393
           HF++ +VA+P A+S V M + GS+L  FWR
Sbjct: 403 HFTNPLVAVPSAVSVVCMALGGSALAVFWR 405

BLAST of MC10g0465 vs. ExPASy Swiss-Prot
Match: Q1EBV7 (Sodium/pyruvate cotransporter BASS2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BASS2 PE=1 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 7.0e-53
Identity = 140/362 (38.67%), Postives = 203/362 (56.08%), Query Frame = 0

Query: 41  AVRSIQGNEEHPSPPPLQKPSGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGP 100
           A   + G+    +P  L   S ++  +    +L+PL+V  G +V   KPS  +W      
Sbjct: 75  AAAGVSGDLPESTPKEL---SQYEKIIELLTTLFPLWVILGTLVGIFKPSLVTWLETD-- 134

Query: 101 ASYSLSLGLIMLAMGLTLELENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPP 160
             ++L LG +ML+MGLTL  E+ F   ++ P ++  G +AQY I P    LI   L L  
Sbjct: 135 -LFTLGLGFLMLSMGLTLTFED-FRRCLRNPWTVGVGFLAQYMIKPILGFLIAMTLKLSA 194

Query: 161 SLSVGLILLGCCPGGTASNVVTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVP 220
            L+ GLIL+ CCPGG ASNV T I++G+V LS++MT C+T+GA+I+TP LTK LAG  VP
Sbjct: 195 PLATGLILVSCCPGGQASNVATYISKGNVALSVLMTTCSTIGAIIMTPLLTKLLAGQLVP 254

Query: 221 IDAAKLSLSTLQVVVAPILFGSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVV 280
           +DAA L+LST QVV+ P + G    + FP     +I   PL+ V+ ++LL          
Sbjct: 255 VDAAGLALSTFQVVLVPTIIGVLANEFFPKFTSKIITVTPLIGVILTTLLC--------- 314

Query: 281 RLKSSMVSATLASDASPWIVLKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERE 340
                         ASP   + +++  +   +IL V LLH A F +GY  +    + E  
Sbjct: 315 --------------ASPIGQVADVLKTQGAQLILPVALLHAAAFAIGYWISKF-SFGEST 374

Query: 341 RRAISIEVGMQNSSLGVVLATSHFSSTMVALPPAMSAVIMNIMGSSLGFFWR-LIQPSDK 400
            R ISIE GMQ+S+LG +LA  HF++ +VA+P A+S V M + GS L  FWR L  P+D 
Sbjct: 375 SRTISIECGMQSSALGFLLAQKHFTNPLVAVPSAVSVVCMALGGSGLAVFWRNLPIPADD 405

Query: 401 EE 402
           ++
Sbjct: 435 KD 405

BLAST of MC10g0465 vs. ExPASy Swiss-Prot
Match: Q7XVB3 (Probable sodium/metabolite cotransporter BASS1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BASS1 PE=2 SV=2)

HSP 1 Score: 206.8 bits (525), Expect = 4.5e-52
Identity = 132/326 (40.49%), Postives = 187/326 (57.36%), Query Frame = 0

Query: 74  YPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLELENLFNLFMQRPLS 133
           +P++V     VA  +P +F W     P +  + +   ML MG+TL L++L    +  P  
Sbjct: 106 FPVWVASACAVALWRPPAFLWV---SPMAQIVGISFTMLGMGMTLTLDDLKTALLM-PKE 165

Query: 134 ILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNVVTLIAQGDVPLSI 193
           +  G + QY++MP +  LI K L LP   + GLIL+ CCPGGTASN+VT +A+G+V LS+
Sbjct: 166 LASGFLLQYSVMPLSGFLISKLLNLPSYYAAGLILVSCCPGGTASNIVTYLARGNVALSV 225

Query: 194 VMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILFGSYLQKVFPSLVK 253
           +MT  +T  A  LTP LT  LAG YV +D   L +ST QVV+AP+L G+ L +    LV+
Sbjct: 226 LMTAASTFAAAFLTPLLTSKLAGQYVAVDPMGLFVSTSQVVLAPVLLGALLNQYCNGLVQ 285

Query: 254 LVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIVLKNIISGELGVVI 313
           LV P  P +AV T ++L  +  ++N         SA L+S                  V+
Sbjct: 286 LVSPLMPFIAVATVAVLCGNAIAQN--------ASAILSSGLQ---------------VV 345

Query: 314 LSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLATSHFSSTMVALPP 373
           +SV  LH +GFF GY+ +   G      R ISIEVGMQNS LGVVLA+ HF + + A+P 
Sbjct: 346 MSVCWLHASGFFFGYVLSRTIGIDISSSRTISIEVGMQNSVLGVVLASKHFGNPLTAVPC 404

Query: 374 AMSAVIMNIMGSSLGFFWRLIQPSDK 400
           A+S+V  ++ GS L   WR + P+DK
Sbjct: 406 AVSSVCHSVYGSLLAGIWRSLPPNDK 404

BLAST of MC10g0465 vs. ExPASy Swiss-Prot
Match: O34524 (Uncharacterized sodium-dependent transporter YocS OS=Bacillus subtilis (strain 168) OX=224308 GN=yocS PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.9e-38
Identity = 115/324 (35.49%), Postives = 185/324 (57.10%), Query Frame = 0

Query: 70  AASLYPLYVTGGGIVACLKPSSFSWFVQGGPASY-SLSLGLIMLAMGLTLELENLFNLFM 129
           A   + ++V    ++    PS F+W      +SY ++ LG+IM  MGLTL+ ++ F   +
Sbjct: 11  AGQTFGIWVIVFAVLGFSFPSLFTWI-----SSYITIFLGIIMFGMGLTLQADD-FKELV 70

Query: 130 QRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNVVTLIAQGD 189
           ++P  ++ G +AQYTIMP  A  +   L LP  ++VG+IL+GCCPGGTASNV+T +A+G+
Sbjct: 71  RKPWQVIIGVIAQYTIMPLVAFGLAFGLHLPAEIAVGVILVGCCPGGTASNVMTFLAKGN 130

Query: 190 VPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILFGSYLQKVF 249
             LS+ +T  +TL A ++TP L    A  ++P+    L +S LQ V+ PI+ G  ++  F
Sbjct: 131 TALSVAVTTISTLLAPVVTPLLIMLFAKEWLPVSPGSLFISILQAVLFPIIAGLIVKMFF 190

Query: 250 PSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIVLKNIISGE 309
              V   +   PLV+V+             +V + S++VS             +N++  +
Sbjct: 191 RKQVAKAVHALPLVSVI------------GIVAIVSAVVSGN----------RENLL--Q 250

Query: 310 LGVVILSVFLLHFA-GFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLATSHFSST 369
            G++I SV +LH   G+ +G++ A +       ++AI+IEVGMQNS LG  LAT+HF S 
Sbjct: 251 SGLLIFSVVILHNGIGYLLGFLCAKLLKMDYPSQKAIAIEVGMQNSGLGAALATAHF-SP 303

Query: 370 MVALPPAMSAVIMNIMGSSLGFFW 392
           + A+P A+ +V  N+ GS L  +W
Sbjct: 311 LSAVPSAIFSVWHNLSGSMLATYW 303

BLAST of MC10g0465 vs. NCBI nr
Match: XP_022136953.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 763 bits (1969), Expect = 5.39e-277
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MSLISLQLTPSISPPYPHRNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQKP 60
           MSLISLQLTPSISPPYPHRNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQKP
Sbjct: 1   MSLISLQLTPSISPPYPHRNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQKP 60

Query: 61  SGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLEL 120
           SGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLEL
Sbjct: 61  SGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLEL 120

Query: 121 ENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNV 180
           ENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNV
Sbjct: 121 ENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNV 180

Query: 181 VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILF 240
           VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILF
Sbjct: 181 VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILF 240

Query: 241 GSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIV 300
           GSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIV
Sbjct: 241 GSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIV 300

Query: 301 LKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLA 360
           LKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLA
Sbjct: 301 LKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLA 360

Query: 361 TSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKEETSAAAAAAK 410
           TSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKEETSAAAAAAK
Sbjct: 361 TSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKEETSAAAAAAK 410

BLAST of MC10g0465 vs. NCBI nr
Match: XP_022136954.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 739 bits (1909), Expect = 5.55e-268
Identity = 402/410 (98.05%), Postives = 402/410 (98.05%), Query Frame = 0

Query: 1   MSLISLQLTPSISPPYPHRNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQKP 60
           MSLISLQLTPSISPPYPHRNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQKP
Sbjct: 1   MSLISLQLTPSISPPYPHRNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQKP 60

Query: 61  SGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLEL 120
           SGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLEL
Sbjct: 61  SGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLEL 120

Query: 121 ENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNV 180
           ENLFNLFMQRPLS        YTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNV
Sbjct: 121 ENLFNLFMQRPLS--------YTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNV 180

Query: 181 VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILF 240
           VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILF
Sbjct: 181 VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILF 240

Query: 241 GSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIV 300
           GSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIV
Sbjct: 241 GSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIV 300

Query: 301 LKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLA 360
           LKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLA
Sbjct: 301 LKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLA 360

Query: 361 TSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKEETSAAAAAAK 410
           TSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKEETSAAAAAAK
Sbjct: 361 TSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKEETSAAAAAAK 402

BLAST of MC10g0465 vs. NCBI nr
Match: XP_038903139.1 (probable sodium/metabolite cotransporter BASS1, chloroplastic [Benincasa hispida])

HSP 1 Score: 610 bits (1572), Expect = 1.45e-216
Identity = 335/405 (82.72%), Postives = 359/405 (88.64%), Query Frame = 0

Query: 1   MSLISLQLTPSISPP-YPHRNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQK 60
           M+ ISLQLTP ISP  +  RNL L RP    LSP K PRS+ VRS+  N EHPSP   +K
Sbjct: 1   MAPISLQLTPFISPLLHRRRNLSLRRPPIPSLSPPKLPRSLPVRSVHRNNEHPSPSHPRK 60

Query: 61  PSGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLE 120
            +G D+FLSTAASLYPLYVT GGIVACLKPS+FSWFVQ GPASYS SLGLIMLAMGLTLE
Sbjct: 61  STGLDDFLSTAASLYPLYVTAGGIVACLKPSTFSWFVQRGPASYSFSLGLIMLAMGLTLE 120

Query: 121 LENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASN 180
           L++LFNLFMQRPLSILFGCVAQYTIMPA+AALIGKF GL PSL VGLILLGCCPGGTASN
Sbjct: 121 LKDLFNLFMQRPLSILFGCVAQYTIMPASAALIGKFFGLSPSLLVGLILLGCCPGGTASN 180

Query: 181 VVTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPIL 240
           VVTLIAQGDVPLSIVMTVCTTLGAVI TPFLTK LAGAY+P+DAA+LSLSTLQVVVAPIL
Sbjct: 181 VVTLIAQGDVPLSIVMTVCTTLGAVIFTPFLTKILAGAYIPVDAAQLSLSTLQVVVAPIL 240

Query: 241 FGSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWI 300
            GS LQK FPSLVKLV+PFAPLVAVLTSSLLACSVFSENV R KSSMV ATLASDAS W 
Sbjct: 241 LGSCLQKAFPSLVKLVLPFAPLVAVLTSSLLACSVFSENVARFKSSMVIATLASDASLWT 300

Query: 301 VLKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVL 360
           VL++I+SGELGVVILSVF LHFAGFFVGYI A IGG+RERERRAISIEVGMQNSSLGV+L
Sbjct: 301 VLQSILSGELGVVILSVFCLHFAGFFVGYITAAIGGFRERERRAISIEVGMQNSSLGVIL 360

Query: 361 ATSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKEETSA 404
           ATSHFSS MVALPPAMSAVIMNIMGS+LGF WR I+PSD+ +TSA
Sbjct: 361 ATSHFSSAMVALPPAMSAVIMNIMGSTLGFCWRYIEPSDEVKTSA 405

BLAST of MC10g0465 vs. NCBI nr
Match: XP_022985149.1 (probable sodium/metabolite cotransporter BASS2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 608 bits (1567), Expect = 8.65e-216
Identity = 330/408 (80.88%), Postives = 362/408 (88.73%), Query Frame = 0

Query: 1   MSLISLQLTPSISPPYPHRNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQKP 60
           M+ ISLQ TP ISP +  RNLRLHRP    L P K PR +AVRS+Q N E+PSP P  KP
Sbjct: 1   MASISLQFTPFISPLHHRRNLRLHRPKIPCLLPPKLPRLLAVRSVQRNNEYPSPLPPAKP 60

Query: 61  SGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLEL 120
           SG D+FLSTAASLYPLYVT GG+VACLKPS+FSWFV+ GP SYSL+LGLIMLAMGLTLEL
Sbjct: 61  SGLDDFLSTAASLYPLYVTVGGVVACLKPSTFSWFVERGPTSYSLALGLIMLAMGLTLEL 120

Query: 121 ENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNV 180
           ++L NLFMQRPLSILFGCVAQYTIMPAA ALIGKF GL   LSVGLILL CCPGGTASNV
Sbjct: 121 KDLVNLFMQRPLSILFGCVAQYTIMPAAGALIGKFFGLSTPLSVGLILLSCCPGGTASNV 180

Query: 181 VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILF 240
           VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTK LAGAY+P+DAAKLSLSTLQVVVAPIL 
Sbjct: 181 VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKLLAGAYIPVDAAKLSLSTLQVVVAPILL 240

Query: 241 GSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIV 300
           GSYLQK FP LVKLVIPFAPLVAVLTSSLLACSVFSENVVR KSSMV+A+LASDASPW++
Sbjct: 241 GSYLQKAFPQLVKLVIPFAPLVAVLTSSLLACSVFSENVVRFKSSMVNASLASDASPWMI 300

Query: 301 LKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLA 360
           +++I+SGELG+V+LSVF LHFAGFFVGY+AA+IGG+RERERRAISIEVGMQNSSLGVVLA
Sbjct: 301 IRSILSGELGMVVLSVFCLHFAGFFVGYVAASIGGFRERERRAISIEVGMQNSSLGVVLA 360

Query: 361 TSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPS-DKEETSAAAA 407
           +SHFSS MVALP AMSAVIMNIMGS+LG  WR I+PS D+ E S + A
Sbjct: 361 SSHFSSAMVALPAAMSAVIMNIMGSTLGLCWRYIEPSADEVEASGSVA 408

BLAST of MC10g0465 vs. NCBI nr
Match: XP_022929395.1 (probable sodium/metabolite cotransporter BASS2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 606 bits (1563), Expect = 3.78e-215
Identity = 330/402 (82.09%), Postives = 358/402 (89.05%), Query Frame = 0

Query: 1   MSLISLQLTPSISPPYPH--RNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQ 60
           M+ ISLQ TP ISP + H  RNLRLHRP    L P K PR +AVRS+Q N E+PSP P  
Sbjct: 1   MASISLQFTPFISPLHHHHRRNLRLHRPKIPCLLPPKLPRLLAVRSVQRNNEYPSPLPPA 60

Query: 61  KPSGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTL 120
           KPSG D+FLSTAASLYPLYVT GG+VACLKPS+FSWFV+ GP SYSL+LGLIMLAMGLTL
Sbjct: 61  KPSGLDDFLSTAASLYPLYVTVGGVVACLKPSTFSWFVERGPTSYSLALGLIMLAMGLTL 120

Query: 121 ELENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTAS 180
           EL++L NLFMQRPLSILFGCVAQYTIMPAA ALIGKF GL  SLSVGLILL CCPGGTAS
Sbjct: 121 ELKDLVNLFMQRPLSILFGCVAQYTIMPAAGALIGKFFGLSTSLSVGLILLSCCPGGTAS 180

Query: 181 NVVTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPI 240
           NVVTLIAQGDVPLSIVMTVCTTLGAVILTPFLTK LAGAY+P+DAAKLSLSTLQVVVAPI
Sbjct: 181 NVVTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKLLAGAYIPVDAAKLSLSTLQVVVAPI 240

Query: 241 LFGSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPW 300
           L GSYLQK FP LVKLVIPFAPLVAVLTSSLLACSVFSENVVR KSSMV+A+LASDASPW
Sbjct: 241 LLGSYLQKAFPQLVKLVIPFAPLVAVLTSSLLACSVFSENVVRFKSSMVNASLASDASPW 300

Query: 301 IVLKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVV 360
           +V+++I+SGELG VILSVF LHFAGFFVGYIAA+IGG+RERERRAISIEVGMQNSSLGVV
Sbjct: 301 MVIRSILSGELGTVILSVFCLHFAGFFVGYIAASIGGFRERERRAISIEVGMQNSSLGVV 360

Query: 361 LATSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKE 400
           LA+SHFSS MVALP AMSAV+MNIMGS+LG  WR I+P+  E
Sbjct: 361 LASSHFSSAMVALPAAMSAVLMNIMGSTLGLCWRYIEPAADE 402

BLAST of MC10g0465 vs. ExPASy TrEMBL
Match: A0A6J1C8Y1 (probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008522 PE=3 SV=1)

HSP 1 Score: 763 bits (1969), Expect = 2.61e-277
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   MSLISLQLTPSISPPYPHRNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQKP 60
           MSLISLQLTPSISPPYPHRNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQKP
Sbjct: 1   MSLISLQLTPSISPPYPHRNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQKP 60

Query: 61  SGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLEL 120
           SGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLEL
Sbjct: 61  SGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLEL 120

Query: 121 ENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNV 180
           ENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNV
Sbjct: 121 ENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNV 180

Query: 181 VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILF 240
           VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILF
Sbjct: 181 VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILF 240

Query: 241 GSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIV 300
           GSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIV
Sbjct: 241 GSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIV 300

Query: 301 LKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLA 360
           LKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLA
Sbjct: 301 LKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLA 360

Query: 361 TSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKEETSAAAAAAK 410
           TSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKEETSAAAAAAK
Sbjct: 361 TSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKEETSAAAAAAK 410

BLAST of MC10g0465 vs. ExPASy TrEMBL
Match: A0A6J1C5S7 (probable sodium/metabolite cotransporter BASS1, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008522 PE=3 SV=1)

HSP 1 Score: 739 bits (1909), Expect = 2.68e-268
Identity = 402/410 (98.05%), Postives = 402/410 (98.05%), Query Frame = 0

Query: 1   MSLISLQLTPSISPPYPHRNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQKP 60
           MSLISLQLTPSISPPYPHRNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQKP
Sbjct: 1   MSLISLQLTPSISPPYPHRNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQKP 60

Query: 61  SGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLEL 120
           SGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLEL
Sbjct: 61  SGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLEL 120

Query: 121 ENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNV 180
           ENLFNLFMQRPLS        YTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNV
Sbjct: 121 ENLFNLFMQRPLS--------YTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNV 180

Query: 181 VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILF 240
           VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILF
Sbjct: 181 VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILF 240

Query: 241 GSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIV 300
           GSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIV
Sbjct: 241 GSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIV 300

Query: 301 LKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLA 360
           LKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLA
Sbjct: 301 LKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLA 360

Query: 361 TSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKEETSAAAAAAK 410
           TSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKEETSAAAAAAK
Sbjct: 361 TSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKEETSAAAAAAK 402

BLAST of MC10g0465 vs. ExPASy TrEMBL
Match: A0A6J1JCH6 (probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483238 PE=3 SV=1)

HSP 1 Score: 608 bits (1567), Expect = 4.19e-216
Identity = 330/408 (80.88%), Postives = 362/408 (88.73%), Query Frame = 0

Query: 1   MSLISLQLTPSISPPYPHRNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQKP 60
           M+ ISLQ TP ISP +  RNLRLHRP    L P K PR +AVRS+Q N E+PSP P  KP
Sbjct: 1   MASISLQFTPFISPLHHRRNLRLHRPKIPCLLPPKLPRLLAVRSVQRNNEYPSPLPPAKP 60

Query: 61  SGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLEL 120
           SG D+FLSTAASLYPLYVT GG+VACLKPS+FSWFV+ GP SYSL+LGLIMLAMGLTLEL
Sbjct: 61  SGLDDFLSTAASLYPLYVTVGGVVACLKPSTFSWFVERGPTSYSLALGLIMLAMGLTLEL 120

Query: 121 ENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNV 180
           ++L NLFMQRPLSILFGCVAQYTIMPAA ALIGKF GL   LSVGLILL CCPGGTASNV
Sbjct: 121 KDLVNLFMQRPLSILFGCVAQYTIMPAAGALIGKFFGLSTPLSVGLILLSCCPGGTASNV 180

Query: 181 VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILF 240
           VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTK LAGAY+P+DAAKLSLSTLQVVVAPIL 
Sbjct: 181 VTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKLLAGAYIPVDAAKLSLSTLQVVVAPILL 240

Query: 241 GSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIV 300
           GSYLQK FP LVKLVIPFAPLVAVLTSSLLACSVFSENVVR KSSMV+A+LASDASPW++
Sbjct: 241 GSYLQKAFPQLVKLVIPFAPLVAVLTSSLLACSVFSENVVRFKSSMVNASLASDASPWMI 300

Query: 301 LKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLA 360
           +++I+SGELG+V+LSVF LHFAGFFVGY+AA+IGG+RERERRAISIEVGMQNSSLGVVLA
Sbjct: 301 IRSILSGELGMVVLSVFCLHFAGFFVGYVAASIGGFRERERRAISIEVGMQNSSLGVVLA 360

Query: 361 TSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPS-DKEETSAAAA 407
           +SHFSS MVALP AMSAVIMNIMGS+LG  WR I+PS D+ E S + A
Sbjct: 361 SSHFSSAMVALPAAMSAVIMNIMGSTLGLCWRYIEPSADEVEASGSVA 408

BLAST of MC10g0465 vs. ExPASy TrEMBL
Match: A0A6J1EUA3 (probable sodium/metabolite cotransporter BASS2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111435979 PE=3 SV=1)

HSP 1 Score: 606 bits (1563), Expect = 1.83e-215
Identity = 330/402 (82.09%), Postives = 358/402 (89.05%), Query Frame = 0

Query: 1   MSLISLQLTPSISPPYPH--RNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQ 60
           M+ ISLQ TP ISP + H  RNLRLHRP    L P K PR +AVRS+Q N E+PSP P  
Sbjct: 1   MASISLQFTPFISPLHHHHRRNLRLHRPKIPCLLPPKLPRLLAVRSVQRNNEYPSPLPPA 60

Query: 61  KPSGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTL 120
           KPSG D+FLSTAASLYPLYVT GG+VACLKPS+FSWFV+ GP SYSL+LGLIMLAMGLTL
Sbjct: 61  KPSGLDDFLSTAASLYPLYVTVGGVVACLKPSTFSWFVERGPTSYSLALGLIMLAMGLTL 120

Query: 121 ELENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTAS 180
           EL++L NLFMQRPLSILFGCVAQYTIMPAA ALIGKF GL  SLSVGLILL CCPGGTAS
Sbjct: 121 ELKDLVNLFMQRPLSILFGCVAQYTIMPAAGALIGKFFGLSTSLSVGLILLSCCPGGTAS 180

Query: 181 NVVTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPI 240
           NVVTLIAQGDVPLSIVMTVCTTLGAVILTPFLTK LAGAY+P+DAAKLSLSTLQVVVAPI
Sbjct: 181 NVVTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKLLAGAYIPVDAAKLSLSTLQVVVAPI 240

Query: 241 LFGSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPW 300
           L GSYLQK FP LVKLVIPFAPLVAVLTSSLLACSVFSENVVR KSSMV+A+LASDASPW
Sbjct: 241 LLGSYLQKAFPQLVKLVIPFAPLVAVLTSSLLACSVFSENVVRFKSSMVNASLASDASPW 300

Query: 301 IVLKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVV 360
           +V+++I+SGELG VILSVF LHFAGFFVGYIAA+IGG+RERERRAISIEVGMQNSSLGVV
Sbjct: 301 MVIRSILSGELGTVILSVFCLHFAGFFVGYIAASIGGFRERERRAISIEVGMQNSSLGVV 360

Query: 361 LATSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKE 400
           LA+SHFSS MVALP AMSAV+MNIMGS+LG  WR I+P+  E
Sbjct: 361 LASSHFSSAMVALPAAMSAVLMNIMGSTLGLCWRYIEPAADE 402

BLAST of MC10g0465 vs. ExPASy TrEMBL
Match: A0A0A0L3A1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G106000 PE=3 SV=1)

HSP 1 Score: 594 bits (1532), Expect = 7.39e-211
Identity = 325/403 (80.65%), Postives = 355/403 (88.09%), Query Frame = 0

Query: 4   ISLQLTPSISPPYPHRNLRLHRPLNLRLSPSKTPRSVAVRSIQGNEEHPSPPPLQKPSGF 63
           ISLQLTP ISP     NLRLHRP    LSP   PRS+ VRS+Q N EHPSP P  KP+G 
Sbjct: 4   ISLQLTPFISPLIHRPNLRLHRPPIPPLSP---PRSLTVRSVQQNNEHPSPSPPPKPTGL 63

Query: 64  DNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLELENL 123
           D+FLSTAASLYPLYVT GGIVACL+PS+FSWFVQ GP+SYSLSLGLIMLAMGLTLE+++L
Sbjct: 64  DDFLSTAASLYPLYVTAGGIVACLEPSTFSWFVQRGPSSYSLSLGLIMLAMGLTLEIKDL 123

Query: 124 FNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNVVTL 183
           FNLFMQRPLSILFGCVAQYTIMPA+A LIGK LGL  SL  GL+LLGCCPGG+ASNVVTL
Sbjct: 124 FNLFMQRPLSILFGCVAQYTIMPASAVLIGKLLGLSQSLLFGLVLLGCCPGGSASNVVTL 183

Query: 184 IAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILFGSY 243
           IAQGDVPLSIVMTVCTTLGAVI TPFLTK L GAY+P+DAA+LSLSTLQVVVAPIL GS 
Sbjct: 184 IAQGDVPLSIVMTVCTTLGAVIFTPFLTKFLVGAYIPVDAAQLSLSTLQVVVAPILLGSC 243

Query: 244 LQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIVLKN 303
           LQK FPSLVKLV+PFAPLVAVLTSSLLA SVFSENV+R+KSSMVSATLASDAS W VLK+
Sbjct: 244 LQKAFPSLVKLVLPFAPLVAVLTSSLLASSVFSENVIRIKSSMVSATLASDASLWTVLKS 303

Query: 304 IISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQNSSLGVVLATSH 363
           I+SGELGVVILSVF LHFAGFFVGYIAA I G+RERERR IS++VGMQNSSLGVVLA SH
Sbjct: 304 ILSGELGVVILSVFCLHFAGFFVGYIAAAICGFRERERRTISMQVGMQNSSLGVVLAASH 363

Query: 364 FSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKEETSAAA 406
           FSS MVALPPA+SAVIMN+MGS+LGF W+ IQPSD+ +TS  A
Sbjct: 364 FSSAMVALPPAISAVIMNMMGSTLGFCWKYIQPSDEVKTSVVA 403

BLAST of MC10g0465 vs. TAIR 10
Match: AT1G78560.1 (Sodium Bile acid symporter family )

HSP 1 Score: 218.4 bits (555), Expect = 1.1e-56
Identity = 140/350 (40.00%), Postives = 202/350 (57.71%), Query Frame = 0

Query: 56  PLQKPSGFDNFL----STAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIM 115
           P +K   F  ++       ++ +P++V+ G ++  ++PS+F+W     P    + L + M
Sbjct: 79  PTEKKKSFGEWVEFVGEAVSTAFPIWVSLGCLLGLMRPSTFNWVT---PNWTIVGLTITM 138

Query: 116 LAMGLTLELENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGC 175
           L MG+TL L++L    +  P  +  G + QY++MP +A  + K L LPP  + GLIL+GC
Sbjct: 139 LGMGMTLTLDDLRGA-LSMPKELFAGFLLQYSVMPLSAFFVSKLLNLPPHYAAGLILVGC 198

Query: 176 CPGGTASNVVTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTL 235
           CPGGTASN+VT IA+G+V LS++MT  +T+ AVI+TP LT  LA  Y+ +DA  L +STL
Sbjct: 199 CPGGTASNIVTYIARGNVALSVLMTAASTVSAVIMTPLLTAKLAKQYITVDALGLLMSTL 258

Query: 236 QVVVAPILFGSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATL 295
           QVV+ P+L G++L + F  LVK V P  P +AV T ++L      +N         SA L
Sbjct: 259 QVVLLPVLAGAFLNQYFKKLVKFVSPVMPPIAVGTVAILCGYAIGQN--------ASAIL 318

Query: 296 ASDASPWIVLKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERERRAISIEVGMQ 355
            S                  V+L+  LLH +GF  GY+ + I G      R ISIEVGMQ
Sbjct: 319 MSGKQ---------------VVLASCLLHISGFLFGYLFSRILGIDVASSRTISIEVGMQ 378

Query: 356 NSSLGVVLATSHFSSTMVALPPAMSAVIMNIMGSSLGFFWRLIQPSDKEE 402
           NS LGVVLAT HF + + A+P A+S+V  +I+GS L   WR   P   E+
Sbjct: 379 NSVLGVVLATQHFGNPLTAVPCAVSSVCHSILGSVLAGIWRRSAPKQLED 401

BLAST of MC10g0465 vs. TAIR 10
Match: AT2G26900.1 (Sodium Bile acid symporter family )

HSP 1 Score: 209.5 bits (532), Expect = 5.0e-54
Identity = 140/362 (38.67%), Postives = 203/362 (56.08%), Query Frame = 0

Query: 41  AVRSIQGNEEHPSPPPLQKPSGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGP 100
           A   + G+    +P  L   S ++  +    +L+PL+V  G +V   KPS  +W      
Sbjct: 75  AAAGVSGDLPESTPKEL---SQYEKIIELLTTLFPLWVILGTLVGIFKPSLVTWLETD-- 134

Query: 101 ASYSLSLGLIMLAMGLTLELENLFNLFMQRPLSILFGCVAQYTIMPAAAALIGKFLGLPP 160
             ++L LG +ML+MGLTL  E+ F   ++ P ++  G +AQY I P    LI   L L  
Sbjct: 135 -LFTLGLGFLMLSMGLTLTFED-FRRCLRNPWTVGVGFLAQYMIKPILGFLIAMTLKLSA 194

Query: 161 SLSVGLILLGCCPGGTASNVVTLIAQGDVPLSIVMTVCTTLGAVILTPFLTKTLAGAYVP 220
            L+ GLIL+ CCPGG ASNV T I++G+V LS++MT C+T+GA+I+TP LTK LAG  VP
Sbjct: 195 PLATGLILVSCCPGGQASNVATYISKGNVALSVLMTTCSTIGAIIMTPLLTKLLAGQLVP 254

Query: 221 IDAAKLSLSTLQVVVAPILFGSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVV 280
           +DAA L+LST QVV+ P + G    + FP     +I   PL+ V+ ++LL          
Sbjct: 255 VDAAGLALSTFQVVLVPTIIGVLANEFFPKFTSKIITVTPLIGVILTTLLC--------- 314

Query: 281 RLKSSMVSATLASDASPWIVLKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERE 340
                         ASP   + +++  +   +IL V LLH A F +GY  +    + E  
Sbjct: 315 --------------ASPIGQVADVLKTQGAQLILPVALLHAAAFAIGYWISKF-SFGEST 374

Query: 341 RRAISIEVGMQNSSLGVVLATSHFSSTMVALPPAMSAVIMNIMGSSLGFFWR-LIQPSDK 400
            R ISIE GMQ+S+LG +LA  HF++ +VA+P A+S V M + GS L  FWR L  P+D 
Sbjct: 375 SRTISIECGMQSSALGFLLAQKHFTNPLVAVPSAVSVVCMALGGSGLAVFWRNLPIPADD 405

Query: 401 EE 402
           ++
Sbjct: 435 KD 405

BLAST of MC10g0465 vs. TAIR 10
Match: AT4G22840.1 (Sodium Bile acid symporter family )

HSP 1 Score: 141.7 bits (356), Expect = 1.3e-33
Identity = 113/345 (32.75%), Postives = 174/345 (50.43%), Query Frame = 0

Query: 57  LQKPSGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGL 116
           ++KPS  D  +  A S+ P  V    I+A + P SF+WF       +  +LG +M A+G+
Sbjct: 85  IEKPSIVDR-MKKANSILPHVVLASTILALIYPPSFTWFTS---RYFVPALGFLMFAVGI 144

Query: 117 TLELENLFNLFMQRPLSILFGCVAQYTIMPAAAALIG----KFLGLPPSLSVGLILLGCC 176
               ++    F +RP +IL G V QY + P    + G        LP  +  G++L+ C 
Sbjct: 145 NSNEKDFLEAF-KRPKAILLGYVGQYLVKPVLGFIFGLAAVSLFQLPTPIGAGIMLVSCV 204

Query: 177 PGGTASNVVTLIAQGDV-PLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTL 236
            G   SN  T +    + PLSIVMT  +T  AV++TP L+  L G  +P+D   +  S L
Sbjct: 205 SGAQLSNYATFLTDPALAPLSIVMTSLSTATAVLVTPMLSLLLIGKKLPVDVKGMISSIL 264

Query: 237 QVVVAPILFGSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATL 296
           QVV+API  G  L K+FP +   + PF P+++VL +   AC              V A L
Sbjct: 265 QVVIAPIAAGLLLNKLFPKVSNAIRPFLPILSVLDT---AC-------------CVGAPL 324

Query: 297 ASDASPWIVLKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYR-----ERERRAISI 356
           A      + + +++S     ++L V + H + F  GY   T   +R     +  +R +S 
Sbjct: 325 A------LNINSVMSPFGATILLLVTMFHLSAFLAGYF-LTGSVFRNAPDAKAMQRTLSY 384

Query: 357 EVGMQNSSLGVVLATSHFSSTMVALPPAMSAVIMNIMGSSLGFFW 392
           E GMQ+S L + LAT  F   +V +PPA+S V+M++MG +L   W
Sbjct: 385 ETGMQSSLLALALATKFFQDPLVGIPPAISTVVMSLMGFTLVLIW 401

BLAST of MC10g0465 vs. TAIR 10
Match: AT3G25410.1 (Sodium Bile acid symporter family )

HSP 1 Score: 138.3 bits (347), Expect = 1.4e-32
Identity = 110/323 (34.06%), Postives = 164/323 (50.77%), Query Frame = 0

Query: 71  ASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLTLELENLFNLFMQR 130
           ++L P  V    + A   P SF+W  +     Y+ +LG IML++G+ L +++ F L  +R
Sbjct: 112 SALLPFVVALTAVAALSYPPSFTWVSKD---LYAPALGGIMLSIGIQLSVDD-FALAFKR 171

Query: 131 PLSILFGCVAQYTIMPAAAALIGKFLGLPPSLSVGLILLGCCPGGTASNVVTLIAQGDVP 190
           P+ +  G VAQY + P    L+    G+P +   G IL  C  G   S+  + +++ DV 
Sbjct: 172 PVPLSVGFVAQYVLKPLLGVLVANAFGMPRTFYAGFILTCCVAGAQLSSYASSLSKADVA 231

Query: 191 LSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQVVVAPILFGSYLQKVFPS 250
           +SI++T  TT+ +VI TP L+  L G+ VP+DA  +S S LQVV+ PI  G  L      
Sbjct: 232 MSILLTSSTTIASVIFTPLLSGLLIGSVVPVDAVAMSKSILQVVLVPITLGLVLNTYAKP 291

Query: 251 LVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLASDASPWIVLKNIISGELG 310
           +V L+ P  P VA++ +SL   S  S N                         I+S E  
Sbjct: 292 VVTLLQPVMPFVAMVCTSLCIGSPLSIN----------------------RSQILSAEGL 351

Query: 311 VVILSVFLLHFAGFFVGYIAATIGGYRERER--RAISIEVGMQNSSLGVVLATSHFSSTM 370
            +I+ +   H   F +GY  + I G R+ E   R IS+  GMQ+S+L  +LA S F  + 
Sbjct: 352 GLIVPIVTFHAVAFALGYWFSKIPGLRQEEEVSRTISLCTGMQSSTLAGLLA-SQFLGSS 407

Query: 371 VALPPAMSAVIMNIMGSSLGFFW 392
            A+P A S V+M IMG  L  FW
Sbjct: 412 QAVPAACSVVVMAIMGLCLASFW 407

BLAST of MC10g0465 vs. TAIR 10
Match: AT4G12030.2 (bile acid transporter 5 )

HSP 1 Score: 126.3 bits (316), Expect = 5.5e-29
Identity = 108/344 (31.40%), Postives = 160/344 (46.51%), Query Frame = 0

Query: 58  QKPSGFDNFLSTAASLYPLYVTGGGIVACLKPSSFSWFVQGGPASYSLSLGLIMLAMGLT 117
           +K S     L  A S  P  +    I+A + P SF+WF    P  +   LG +M A+G+ 
Sbjct: 86  KKVSSIMETLKQAYSFIPHGILLSTILALVYPPSFTWF---KPRYFVPGLGFMMFAVGIN 145

Query: 118 LELENLFNLFMQRPLSILFGCVAQYTIMPAAAALIG----KFLGLPPSLSVGLILLGCCP 177
              E  F   ++RP +I  G + QY I P    + G        LP S+  G++L+ C  
Sbjct: 146 SN-ERDFLEALKRPDAIFAGYIGQYLIKPLLGYIFGVIAVSLFNLPTSIGAGIMLVSCVS 205

Query: 178 GGTASNVVTLIAQGDV-PLSIVMTVCTTLGAVILTPFLTKTLAGAYVPIDAAKLSLSTLQ 237
           G   SN  T +    +  LSIVMT  +T  AV++TP L+  L G  +P+D   +  S LQ
Sbjct: 206 GAQLSNYTTFLTDPSLAALSIVMTSISTATAVLVTPMLSLLLIGKKLPVDVFGMISSILQ 265

Query: 238 VVVAPILFGSYLQKVFPSLVKLVIPFAPLVAVLTSSLLACSVFSENVVRLKSSMVSATLA 297
           VV+ PI  G  L ++FP L   + PF P + V+                  S  + A LA
Sbjct: 266 VVITPIAAGLLLNRLFPRLSNAIKPFLPALTVID----------------MSCCIGAPLA 325

Query: 298 SDASPWIVLKNIISGELGVVILSVFLLHFAGFFVGYIAATIGGYRERE----RRAISIEV 357
                 + + +I+S     ++  V   H   F  GY        +  +    +R IS E 
Sbjct: 326 ------LNIDSILSPFGATILFLVITFHLLAFVAGYFFTGFFFSKAPDVKALQRTISYET 385

Query: 358 GMQNSSLGVVLATSHFSSTMVALPPAMSAVIMNIMGSSLGFFWR 393
           GMQ+S L + LAT  F   +V +PPA+S V+M++MG SL   W+
Sbjct: 386 GMQSSLLALALATKFFQDPLVGVPPAISTVVMSLMGVSLVTIWK 403