MC10g0444 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g0444
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Serine hydroxymethyltransferase
Location	MC10: 3591126 .. 3595048 (-)
RNA-Seq Expression	MC10g0444
Synteny	MC10g0444

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTTGTCCAATTCCCATTCCAATCTATCTCTCGGGTTCTCGTCTCATGCTTCGCCGCCCCACCGCGCTCAAATTGCTGATGATTCCGTCCTTCTTCATCTCGATTCTACTCGACGGGAACCCCCGTATTCGTCCCCCCCAGTCCCTCTTCAGTTGATGGAACCGCAGACGGAGAGTCACAGAGACGAGGATGGAGAGGCGGAGAGAGAAGACGATGACTACGACGACAGGGAGGTGGAGGAGTTTCGTATTCTGGGTCATTCTATGTGCTTGAAGCGCCGAAGAGATGGCGAATCTTCATCCTCGTCGGGCTCTACCAAGAGGGTTTCGTGTGAATTTGATATGGATGCGAGAAGGAGTGCCGTACGGTCGTGGGGCAACCAGACCCTTCGAGTTGCGGACCCTAACCTATTTGGGATTATGGAGAAGGAGAAACATAGGCAATTCAAGGGAATCGAATTGATTGCTTCCGAAAATTATGTGTGCCGGGCTGTTATGGAAGCACTTGGAAGCCATTTGACTAATAAGTACTCGGAAGGAATGCCTGGTGCGCGGTATTACGGTGGAAATCAATATATAGATGAGATTGAAACCCTTTGCCGTGAGCGCGCATTGGCGGCCTTTGGTCTTGATTCTGATAATTGGGGAGTTAACGTGCAACCTTATTCATGCACGTCCGCGAACTTTGCTGTCTACACTGGTCTGTTACTACCCGGAGATCGAATCATGGGGTTAGATACCCCTTCAGGTGGGAATACAAGTCATGGGTACTGTACCCCAAATGGCAGAAAAGTTTCGGGGGCCTCAATTTTCTTTGAGAGTTTGCCATACAAGGTGAATCCGCAAACTGGGTATATAGATTATGATAAGCTCGAGGAGAGGGCGCTTGACTTTCGCCCAAAAATACTTATTTGTGGTGGGAGCTCATATCCTCGTGAGTTGGATTATGCAAGGTTTAGGCAGATTGCAGATAAGTGTGGAGCAGTTCTAATGTGTGATATGGCTCAAATTAGTGGCCTTGTCGCTGCTAAGGTGTGAACTTTTCTCCATCTTTGCATTGTGTATATATATATATTGAGTTTTATCTGTTCAAAAAAGTTTTAGGTTTCCTTATCTTATGTAATATTGTGTCTTCATTCTTTTGGGCCACAATTTAGTTGGTTGTTGATATAATCAAGATATTTGAATATCTAAACAGATGAAATTTGAAATCTTTGAGAAATGAGAAGTACTTGTGAATGTTTTTTATTCTTTAAGCTTATGTATTGTGGCATTTCTTTTTGCTACTTTTTATCAAAAGATTGTCTTTCCTTTCTACTTGCTTCTATTATACAGTTTGTTTAACTGTGTATTTATTATTTAATCTGTAGAACCTCATTTAAATTTTTTTTTTTTCGTGGAGTGCATGAATTGACAGTCTTTGTTTTGTAATCGTTTCTGTTTGCACTTTCATCACTCAGTGTGAATTTTTATTTCCTTTTTTACAAAATAGTTTGTTGCTTTTACACCCGTTAGGCCCATCAATACCTCATATGTGTAATTTTGTAGTTTTAGGTGGAAAAATTATCTCTACTGTTCCGTTAATATTTTGATTTACATTTTATTACAGGAATGTGTTAGCCCTTTTGGGTACTGTGATGTTGTGACTTCAACTACTCATAAGAGCCTCCGAGGTCCAAGGGGAGGTATAATTTTCTATAGGAAGGGTAGTAAGCCAAGGAAGAGAGCAATGCTTTTGTACCAGGGAAACGAGACTGACCAATATGACTTTGAGGAGAAGATAAACTTTGCTGTTTTTCCATCTTTACAAGGTGGACCACACAATAATCATATTGCTGCTCTTGCGGTTGCCCTAAAACAATTGGCTACTCCAGAATACAAGGCATACATGCAGCAGGTGAAGAAAAATGCTCAAGCCTTGGCTTCTGCTTTACTTCGAAAAAAGTACAGGCTTGTAACTGGTGGCACAGATAATCACATGTTACTCTGGGATCTGAGGCCCTTGGGATTAACAGGTGCATAATCTTGATTCAAATTTTGCAAATTGATGTGAACATTGCATATTTGAGGCACATGGCAAGCTTTTTGTTTTTCTAGAGGCAAGAGGTGCACTAATAGTGTTGCTCAAGTAAAGTGAGGATGGATTATGTTACACATTGTGAATTGTGAATATAGAAGATAGTACCATTTTACAGGCTATATAAAATATGATATTGTAGTAAATTGTGCTAAAAAAGTACTTGATTGATTACAAATATGTTTTTATATAGTAATCTCTGATACTTTTTTTTCTTTTTTATTTTTTAAAAAGAGATTATGAATGAAGTCATGTTTTTGTCAAATACATATTTAATACACTGAATATATTTTTCTAAATTAGTAAAGATGTTGGAAATGATGAAAAGGCAACCAAGGCTTATGACTGTAGGCATGACAAGTCAGAAAGGGTGCTGAGAAATCAACCCAAGAAGCCAAAGAGGAGCTGGAAAAGTTTCTCCAATTGGCAACGATAAATAATGATTGCTTGCAGTTACAAATCCGTTTTGATAAGGAACTCCAAGAAGAAGCCTTGTATTGGATGAACTCTGAAACTTCTTCTTTTCTGGCCAAATTGTGAATCAATCTATGATTTTTTCCTAGTCAAACTTCAAAGAACCCTTCATGGCATCTTTTTTCAGATTTTCTCTGCTTTGTATTTAAAGGGATGCCACGTAGGCAATGATACAGGATTTGCAGAACAGATAAGGGGACACATCAAGCAAAATTTAACTTATCTAGCAGAGTTCCTTGAAAGATTTTGGGTGAAGGAGCAGTGTGAGAATAAGCTATTTGGGTTTCTGAACCGTTTTTATCACAAGCAGGAACAACTAGAAGAGAAGACTCAGGATTTCTTCTTTGTATAAAGCAGCCGTATTTAGGCCCCATAGAAAACTAGGTTCCACAGGACAATTTTTGTCTTAGAGCTTTATGACTCCAGAAAGCCTCTTGTTTAGGATGGTGGATCTGCTGGTTAGTTGGACAAAGAGGGAGCAACTGGTAAATATGCCCCTATCGTCCTACTGCAAAACAATGGATTTGATTTTCAGTTTGGTGTTAGTTCACTATCCATCTCTAGGACTACATCTTATATAGATATGTGTTGCAGAGTCTATCGAGAAATATATGGCTTGTGACTGCAAGATCCCTTTCTCTGGCTTCTTTGTTTATGTTGTACAATGTTTTTTCTTAAATAGAAACATTTCATTGACGAGATGAAATATACAAAAGATGAGGGAGAAGATAGTTTCTGAATGATGTATAATCTATTAGAACTCACAACTTGTTGTTGGTATTCATGCTATCAATGAAATTTGTTTCCTATCCAAAAGAGAAAGAAAGTATTCTATATTTATATACTTATGTTTTTCTTGAGCTCTAGTTCTTGTGGCACCTGTAGCTACTTGATCTATTATTCTCCGTTCATTACATATCATCTATCTTCATGATGGCTTTGTAGGTAAGAACTATGAGAAGGTTTGTGAGATATGCCAAATTACTCTCAACAAAATTACAATATATGGTGATAATGGAATTATTATTCCTGGAGGAGTACGGATTGGTGAGTTATACTCCTATATTTCATGGTTTTGGGGTATATTAAGATTGTAGAGCTTGGTAGGTATTGATTATGTGGTATAAATTAGCTATCTGTTATGCCTGCAGGCACCCCAGCTATGACATCAAGAGGCTGTCTGGAATCTGATTTTGAGACAATTGTTGACTTTCTTCATACAGCCGCTCAAATTGCAATTTCAGTACAGCGGGAGCATGGGAAGCTACCAAAGGCTTATCTTAAGGGCCTTCAAAATAATAATGAAGTTGTTGAGCTCCGAAATCGTGTTGAAAGTTTTTCAGCTCAATTTGCAATGCCGGGATTTGAA

mRNA sequence

ATGGATTTGTCCAATTCCCATTCCAATCTATCTCTCGGGTTCTCGTCTCATGCTTCGCCGCCCCACCGCGCTCAAATTGCTGATGATTCCGTCCTTCTTCATCTCGATTCTACTCGACGGGAACCCCCGTATTCGTCCCCCCCAGTCCCTCTTCAGTTGATGGAACCGCAGACGGAGAGTCACAGAGACGAGGATGGAGAGGCGGAGAGAGAAGACGATGACTACGACGACAGGGAGGTGGAGGAGTTTCGTATTCTGGGTCATTCTATGTGCTTGAAGCGCCGAAGAGATGGCGAATCTTCATCCTCGTCGGGCTCTACCAAGAGGGTTTCGTGTGAATTTGATATGGATGCGAGAAGGAGTGCCGTACGGTCGTGGGGCAACCAGACCCTTCGAGTTGCGGACCCTAACCTATTTGGGATTATGGAGAAGGAGAAACATAGGCAATTCAAGGGAATCGAATTGATTGCTTCCGAAAATTATGTGTGCCGGGCTGTTATGGAAGCACTTGGAAGCCATTTGACTAATAAGTACTCGGAAGGAATGCCTGGTGCGCGGTATTACGGTGGAAATCAATATATAGATGAGATTGAAACCCTTTGCCGTGAGCGCGCATTGGCGGCCTTTGGTCTTGATTCTGATAATTGGGGAGTTAACGTGCAACCTTATTCATGCACGTCCGCGAACTTTGCTGTCTACACTGGTCTGTTACTACCCGGAGATCGAATCATGGGGTTAGATACCCCTTCAGGTGGGAATACAAGTCATGGGTACTGTACCCCAAATGGCAGAAAAGTTTCGGGGGCCTCAATTTTCTTTGAGAGTTTGCCATACAAGGTGAATCCGCAAACTGGGTATATAGATTATGATAAGCTCGAGGAGAGGGCGCTTGACTTTCGCCCAAAAATACTTATTTGTGGTGGGAGCTCATATCCTCGTGAGTTGGATTATGCAAGGTTTAGGCAGATTGCAGATAAGTGTGGAGCAGTTCTAATGTGTGATATGGCTCAAATTAGTGGCCTTGTCGCTGCTAAGGAATGTGTTAGCCCTTTTGGGTACTGTGATGTTGTGACTTCAACTACTCATAAGAGCCTCCGAGGTCCAAGGGGAGGTATAATTTTCTATAGGAAGGGTAGTAAGCCAAGGAAGAGAGCAATGCTTTTGTACCAGGGAAACGAGACTGACCAATATGACTTTGAGGAGAAGATAAACTTTGCTGTTTTTCCATCTTTACAAGGTGGACCACACAATAATCATATTGCTGCTCTTGCGGTTGCCCTAAAACAATTGGCTACTCCAGAATACAAGGCATACATGCAGCAGGTGAAGAAAAATGCTCAAGCCTTGGCTTCTGCTTTACTTCGAAAAAAGTACAGGCTTGTAACTGGTGGCACAGATAATCACATGTTACTCTGGGATCTGAGGCCCTTGGGATTAACAGGTAAGAACTATGAGAAGGTTTGTGAGATATGCCAAATTACTCTCAACAAAATTACAATATATGGTGATAATGGAATTATTATTCCTGGAGGAGTACGGATTGGCACCCCAGCTATGACATCAAGAGGCTGTCTGGAATCTGATTTTGAGACAATTGTTGACTTTCTTCATACAGCCGCTCAAATTGCAATTTCAGTACAGCGGGAGCATGGGAAGCTACCAAAGGCTTATCTTAAGGGCCTTCAAAATAATAATGAAGTTGTTGAGCTCCGAAATCGTGTTGAAAGTTTTTCAGCTCAATTTGCAATGCCGGGATTTGAA

Coding sequence (CDS)

ATGGATTTGTCCAATTCCCATTCCAATCTATCTCTCGGGTTCTCGTCTCATGCTTCGCCGCCCCACCGCGCTCAAATTGCTGATGATTCCGTCCTTCTTCATCTCGATTCTACTCGACGGGAACCCCCGTATTCGTCCCCCCCAGTCCCTCTTCAGTTGATGGAACCGCAGACGGAGAGTCACAGAGACGAGGATGGAGAGGCGGAGAGAGAAGACGATGACTACGACGACAGGGAGGTGGAGGAGTTTCGTATTCTGGGTCATTCTATGTGCTTGAAGCGCCGAAGAGATGGCGAATCTTCATCCTCGTCGGGCTCTACCAAGAGGGTTTCGTGTGAATTTGATATGGATGCGAGAAGGAGTGCCGTACGGTCGTGGGGCAACCAGACCCTTCGAGTTGCGGACCCTAACCTATTTGGGATTATGGAGAAGGAGAAACATAGGCAATTCAAGGGAATCGAATTGATTGCTTCCGAAAATTATGTGTGCCGGGCTGTTATGGAAGCACTTGGAAGCCATTTGACTAATAAGTACTCGGAAGGAATGCCTGGTGCGCGGTATTACGGTGGAAATCAATATATAGATGAGATTGAAACCCTTTGCCGTGAGCGCGCATTGGCGGCCTTTGGTCTTGATTCTGATAATTGGGGAGTTAACGTGCAACCTTATTCATGCACGTCCGCGAACTTTGCTGTCTACACTGGTCTGTTACTACCCGGAGATCGAATCATGGGGTTAGATACCCCTTCAGGTGGGAATACAAGTCATGGGTACTGTACCCCAAATGGCAGAAAAGTTTCGGGGGCCTCAATTTTCTTTGAGAGTTTGCCATACAAGGTGAATCCGCAAACTGGGTATATAGATTATGATAAGCTCGAGGAGAGGGCGCTTGACTTTCGCCCAAAAATACTTATTTGTGGTGGGAGCTCATATCCTCGTGAGTTGGATTATGCAAGGTTTAGGCAGATTGCAGATAAGTGTGGAGCAGTTCTAATGTGTGATATGGCTCAAATTAGTGGCCTTGTCGCTGCTAAGGAATGTGTTAGCCCTTTTGGGTACTGTGATGTTGTGACTTCAACTACTCATAAGAGCCTCCGAGGTCCAAGGGGAGGTATAATTTTCTATAGGAAGGGTAGTAAGCCAAGGAAGAGAGCAATGCTTTTGTACCAGGGAAACGAGACTGACCAATATGACTTTGAGGAGAAGATAAACTTTGCTGTTTTTCCATCTTTACAAGGTGGACCACACAATAATCATATTGCTGCTCTTGCGGTTGCCCTAAAACAATTGGCTACTCCAGAATACAAGGCATACATGCAGCAGGTGAAGAAAAATGCTCAAGCCTTGGCTTCTGCTTTACTTCGAAAAAAGTACAGGCTTGTAACTGGTGGCACAGATAATCACATGTTACTCTGGGATCTGAGGCCCTTGGGATTAACAGGTAAGAACTATGAGAAGGTTTGTGAGATATGCCAAATTACTCTCAACAAAATTACAATATATGGTGATAATGGAATTATTATTCCTGGAGGAGTACGGATTGGCACCCCAGCTATGACATCAAGAGGCTGTCTGGAATCTGATTTTGAGACAATTGTTGACTTTCTTCATACAGCCGCTCAAATTGCAATTTCAGTACAGCGGGAGCATGGGAAGCTACCAAAGGCTTATCTTAAGGGCCTTCAAAATAATAATGAAGTTGTTGAGCTCCGAAATCGTGTTGAAAGTTTTTCAGCTCAATTTGCAATGCCGGGATTTGAA

Protein sequence

MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTESHRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARRSAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLTGKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAAQIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE

Homology

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BLAST of MC10g0444 vs. ExPASy Swiss-Prot
Match: Q84WV0 (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 1.3e-238
Identity = 411/591 (69.54%), Postives = 478/591 (80.88%), Query Frame = 0


Query: 1   MDLSNSHSNLSLGFS-SHAS----PPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLME 60
           MDLS S +N  LGF  SHAS    P  RA IADDS+ L +D + R  P +  P+PLQL+E
Sbjct: 1   MDLSRSQTNFQLGFGCSHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLE 60

Query: 61  PQTESHRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGE-SSSSSGSTKRVSC-E 120
            + E     D   +      D +E E FRILGH MCLKR+RD     + S   KR S  +
Sbjct: 61  QKAEKTTTVDEPKKDGGGGGDQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPKRSSIGD 120

Query: 121 FDMDARRSAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSH 180
            D+++RR+AVR+WG+Q + +ADP++  +MEKEK RQ +GIELIASEN+VCRAVMEALGSH
Sbjct: 121 SDLESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSH 180

Query: 181 LTNKYSEGMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVY 240
           LTNKYSEGMPGARYY GNQYID+IE LC ERAL AFGL+SD WGVNVQPYSCTSANFAVY
Sbjct: 181 LTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVY 240

Query: 241 TGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLE 300
           TGLLLPG+RIMGLD+PSGG+ SHGYCTP G+K+S ASIFFES PYKVNPQTGYIDYDKLE
Sbjct: 241 TGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLE 300

Query: 301 ERALDFRPKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGY 360
           ++ALD+RPKILICGGSSYPR+ D+AR RQIADKCGAVLMCDMA ISGLVA KEC +PF +
Sbjct: 301 DKALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDH 360

Query: 361 CDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQG 420
           CD+VTSTTHK LRGPRGGIIFYR+G K RK+       + +  YD EEKINFAVFPSLQG
Sbjct: 361 CDIVTSTTHKGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQG 420

Query: 421 GPHNNHIAALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWD 480
           GPHNNHIAALA+ALKQ+ATPEYKAY+QQ+KKNAQALA+ALLR+K RLVTGGTDNH+LLWD
Sbjct: 421 GPHNNHIAALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWD 480

Query: 481 LRPLGLTGKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIV 540
           L P+GLTGK YEKVCE+C ITLNK  I+GDNG I PGGVRIGTPAMT+RGC+ESDFET+ 
Sbjct: 481 LTPMGLTGKVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMA 540

Query: 541 DFLHTAAQIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMP 585
           DFL  AAQI  ++QREHGK  K ++K L  N ++ ELRNRVE+F+ Q+ MP
Sbjct: 541 DFLIKAAQITSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

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BLAST of MC10g0444 vs. ExPASy Swiss-Prot
Match: Q9LM59 (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 3.6e-236
Identity = 406/594 (68.35%), Postives = 484/594 (81.48%), Query Frame = 0


Query: 6   SHSNLSLGF-SSHASP---PHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTESH 65
           + S+LSLGF SSHA P   P R  IADDS+ L +DS+ R      PPVPLQL+E + +  
Sbjct: 5   AQSDLSLGFGSSHALPLPHPPRIPIADDSITLQIDSSFRPSSNPMPPVPLQLLEQRFDVT 64

Query: 66  R------DEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSS--SSGSTKRVSCE 125
                  +ED E   ++DD D RE E+F +LGH M LKR R G S S  SS   KR   +
Sbjct: 65  GSCSRVVEEDDEVVGDNDDDDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFVVD 124

Query: 126 FDMDARRSAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSH 185
             +++RR+ VR+WGNQ++  ADP +   MEKEK RQF+GIELIASEN+VCRAVMEALGSH
Sbjct: 125 SGIESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSH 184

Query: 186 LTNKYSEGMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVY 245
           LTNKYSEGMPGARYY GNQYID+IE LC+ERALAAFGL+ + WGVNVQPYSCTSANFAV+
Sbjct: 185 LTNKYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVF 244

Query: 246 TGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLE 305
           TGLL+PG+RIMGLD+PSGG+ SHGY TP G+KVSGASIFFES PYKV+P+TGYIDYDKLE
Sbjct: 245 TGLLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLE 304

Query: 306 ERALDFRPKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGY 365
           E+ALD+RPKILICGGSSYPR+ ++ RFR IADKCGAVLM DMAQISGLVAAKE  +PF Y
Sbjct: 305 EKALDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDY 364

Query: 366 CDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQG 425
           CD+VTSTTHKSLRGPRGGIIFY++G KP+K+++ L       QYDFEEKINF+VFPSLQG
Sbjct: 365 CDIVTSTTHKSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQG 424

Query: 426 GPHNNHIAALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWD 485
           GPHNNHIAALA+ALKQ A+PEYK YM+QVKKNA+ALASAL+ +K +L+TGGTDNH+LLWD
Sbjct: 425 GPHNNHIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWD 484

Query: 486 LRPLGLTGKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIV 545
           L PLGLTGK YEKVCE+C IT+NK+ I+ +NG+I PGGVRIG+PAMTSRGCLE +FET+ 
Sbjct: 485 LTPLGLTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMA 544

Query: 546 DFLHTAAQIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 588
           DFL+ AAQIA + QREHGKL K  LK + +  E+ +LRN+VE+F+ QFAMP F+
Sbjct: 545 DFLYRAAQIASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFD 598

[Close]

BLAST of MC10g0444 vs. ExPASy Swiss-Prot
Match: O23254 (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 3.4e-178
Identity = 300/464 (64.66%), Postives = 359/464 (77.37%), Query Frame = 0


Query: 123 VRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V SWGN +L   DP +  ++EKEK RQ +GIELIASEN+   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 183 PGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYYGGN++IDEIE LCR RAL AF  D   WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPK 302
           IMGLD PSGG+ +HGY T  G+K+S  SI+FESLPYKVN  TGYIDYDKLEE+ALDFRPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 303 ILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 362
           +LICGGS+YPR+ DYARFR IADK GA+L+CDMA ISGLVAA+E  +PF YCDVVT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP K+        E   YDFE+KINFAVFP+LQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PEGAVYDFEDKINFAVFPALQGGPHNHQIGA 303

Query: 423 LAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLTGK 482
           LAVALKQ  TP +K Y +QVK NA AL + L+ K Y++VT GT+NH++LWDLRPLGLTG 
Sbjct: 304 LAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGN 363

Query: 483 NYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAAQI 542
             EK+C++C ITLNK  ++GD+  + PGGVRIG PAMTSRG +E DFE I +FL  A  +
Sbjct: 364 KVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTL 423

Query: 543 AISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGF 587
            + +Q+ +GKL K + KGL NN ++ +L+  VE FSA + MPGF
Sbjct: 424 TLDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGF 462

[Close]

BLAST of MC10g0444 vs. ExPASy Swiss-Prot
Match: Q9SVM4 (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 1.1e-176
Identity = 293/464 (63.15%), Postives = 354/464 (76.29%), Query Frame = 0


Query: 123 VRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V SWGN  L   DP ++ ++EKEKHRQ +GIELIA+EN+   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 183 PGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYYGG ++IDEIE+LCR R+L AF  + + WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPK 302
           IMGLD PSGG+ +HGY +  G+ +S  SI+FE+LPYKV+ +TGYIDYDKLEE+A+DFRPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 303 ILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 362
           ++ICGG+SYPRE DYARFR +ADK GA L+CDMA  S LVAA+E   PF YCDVVT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP K+        E + YDF+ KIN AVFP+LQ GPHNN I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKGQ-----PEGEVYDFDAKINSAVFPALQSGPHNNKIGA 303

Query: 423 LAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLTGK 482
           LAVALKQ+  P +K Y +QVK NA  LAS L+ K Y LVT GTDNH++LWDLRPLGLTG 
Sbjct: 304 LAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGN 363

Query: 483 NYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAAQI 542
             EKVCE+C ITLN+  ++GD   + PGGVRIGTPAMTSRG +E DFE I +FLH A  I
Sbjct: 364 KVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTI 423

Query: 543 AISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGF 587
            + +Q ++GK+ K + KGL NN E+ E++  VE F+  F MPGF
Sbjct: 424 TLDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGF 462

[Close]

BLAST of MC10g0444 vs. ExPASy Swiss-Prot
Match: Q54Z26 (Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 PE=1 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 6.3e-156
Identity = 268/459 (58.39%), Postives = 336/459 (73.20%), Query Frame = 0


Query: 127 GNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGMPGAR 186
           GN  L+  D  +F +M +EK RQFKG+ELIASEN+  RAVMEALGSH TNKY+EG PG+R
Sbjct: 6   GNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSRAVMEALGSHFTNKYAEGYPGSR 65

Query: 187 YYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPGDRIMGL 246
           YYGG + +DE+ETLC++RAL AF LD   WGVNVQPYS + ANFAVYT LL P DRIMGL
Sbjct: 66  YYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYSGSPANFAVYTALLRPHDRIMGL 125

Query: 247 DTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPKILIC 306
           D PSGG+ +HGY T + +K+S +SIFFES+PY++    G IDY +LEE AL F+PK++I 
Sbjct: 126 DLPSGGHLTHGYQT-DKKKISASSIFFESMPYQIGAD-GLIDYQRLEENALLFKPKLIIS 185

Query: 307 GGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTHKSLR 366
           G S+YPRE DY R R IADK GA LMCDMA  SGLVAA+   SPF YCDVVTSTTHK+LR
Sbjct: 186 GASAYPREWDYKRMRAIADKVGAYLMCDMAHYSGLVAAQLLDSPFDYCDVVTSTTHKTLR 245

Query: 367 GPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAALAVA 426
           GPR GIIF+R+G +      +   G E ++YD E KINFAVFPSLQGGPH N IA +AVA
Sbjct: 246 GPRSGIIFFRRGKR------VDGNGKEIEEYDIESKINFAVFPSLQGGPHENVIAGVAVA 305

Query: 427 LKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLTGKNYEK 486
           LK+  + E+K Y  QVKKNA A+ +AL+ K Y+LVT GTDNH++LWDLRP  LTG  +EK
Sbjct: 306 LKEADSQEFKEYALQVKKNAAAIGNALMNKGYKLVTNGTDNHLILWDLRPKELTGNKFEK 365

Query: 487 VCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAAQIAISV 546
             +I  IT+NK  ++GD   I PGG+RIG+ A+TSRG  E+DFE I DFL     I++ +
Sbjct: 366 AADIANITVNKNAVHGDTNAISPGGIRIGSSALTSRGLKEADFEKIADFLDRIVSISLEI 425

Query: 547 QREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPG 586
           Q   GK    ++  +  + E+++LR  VE FS++F +PG
Sbjct: 426 QGRVGKKLVDFVVEINKSKELLDLRKEVEEFSSKFTLPG 456

[Close]

BLAST of MC10g0444 vs. NCBI nr
Match: XP_022149204.1 (serine hydroxymethyltransferase 7-like [Momordica charantia] >XP_022149205.1 serine hydroxymethyltransferase 7-like [Momordica charantia] >XP_022149207.1 serine hydroxymethyltransferase 7-like [Momordica charantia])

HSP 1 Score: 1192 bits (3083), Expect = 0.0
Identity = 587/587 (100.00%), Postives = 587/587 (100.00%), Query Frame = 0


Query: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60
           MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES
Sbjct: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60

Query: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120
           HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR
Sbjct: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120

Query: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT
Sbjct: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540
           GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA
Sbjct: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587
           QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE
Sbjct: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587

[Close]

BLAST of MC10g0444 vs. NCBI nr
Match: XP_022941930.1 (serine hydroxymethyltransferase 7-like [Cucurbita moschata] >KAG6600365.1 Serine hydroxymethyltransferase 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031023.1 Serine hydroxymethyltransferase 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1124 bits (2907), Expect = 0.0
Identity = 551/587 (93.87%), Postives = 570/587 (97.10%), Query Frame = 0


Query: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60
           MDLS SHSNLSLGFSSHASPPHRAQIADDSVLLHLDS+RRE  YSS PVPLQLME QTES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120
           HR++ G+AER+DD+YDDREVEEFRILGHSMCLKRRRDGESS+SSGS KR+SCEFD++ RR
Sbjct: 61  HRNDGGDAERDDDEYDDREVEEFRILGHSMCLKRRRDGESSTSSGSAKRISCEFDIETRR 120

Query: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+QTLRVADPNLFGIMEKEK RQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIETLCRERALAAFGL SDNWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQ+ATPEYKAYMQQVKKNAQALA+ALLRKKY+LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540
           GKNYE VCEIC ITLNKITI+GDNGIIIPGGVR+GTPAMTSRGCLESDFETI DFLHTAA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587
           QIAISVQRE+GKLPK YLKGL +NNEVVELRNRVESFSAQFAMPGFE
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

[Close]

BLAST of MC10g0444 vs. NCBI nr
Match: XP_023547556.1 (serine hydroxymethyltransferase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1120 bits (2898), Expect = 0.0
Identity = 549/587 (93.53%), Postives = 568/587 (96.76%), Query Frame = 0


Query: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60
           MDLS SHSNLSLGFSSHASPPHRAQIADDSVLLHLDS+RRE  YSS PVPLQLME QTES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120
           HR++ G+ ER+DD+YDDREVEEFRILGH MCLKRRRDGESS+SSGS KR+SCEFD++ RR
Sbjct: 61  HRNDGGDGERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSGSAKRISCEFDIETRR 120

Query: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+QTLRVADPNLFGIMEKEK RQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIETLCRERALAAFGL SDNWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQ+ATPEYKAYMQQVKKNAQALA+ALLRKKY+LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540
           GKNYE VCEIC ITLNKITI+GDNGIIIPGGVR+GTPAMTSRGCLESDFETI DFLHTAA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587
           QIAISVQRE+GKLPK YLKGL +NNEVVELRNRVESFSAQFAMPGFE
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

[Close]

BLAST of MC10g0444 vs. NCBI nr
Match: XP_022979958.1 (serine hydroxymethyltransferase 7-like [Cucurbita maxima])

HSP 1 Score: 1118 bits (2892), Expect = 0.0
Identity = 548/587 (93.36%), Postives = 567/587 (96.59%), Query Frame = 0


Query: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60
           MDLS SHSNLSLGFSSHASPPHRAQIADDSVLLHLDS+RRE  YSS PVPLQLME QTES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120
           HR++ G+AER+DD+YDDREVEEFRILGH MCLKRRRDGESS+SS S KR+SCEFD++ RR
Sbjct: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120

Query: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+QTLRVADPNLFGIMEKEK RQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIETLCRERALAAFGL SDNWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYARFRQIADKCGA LMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQ+ATPEYKAYMQQVKKNAQALA+ALLRKKY+LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540
           GKNYE VCEIC ITLNKITI+GDNGIIIPGGVR+GTPAMTSRGCLESDFETI DFLHTAA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587
           QIAISVQRE+GKLPK YLKGL +NNEVVELRNRVESFSAQFAMPGFE
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

[Close]

BLAST of MC10g0444 vs. NCBI nr
Match: KAG6576983.1 (Serine hydroxymethyltransferase 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015003.1 Serine hydroxymethyltransferase 7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1078 bits (2788), Expect = 0.0
Identity = 529/587 (90.12%), Postives = 554/587 (94.38%), Query Frame = 0


Query: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60
           MDLSNSHSNLSLG SSHASPPHR QIADDS+LLHL+S+R+EP +SSPP+PLQLMEP TES
Sbjct: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60

Query: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120
           HRDE G+ EREDD YDDR+VEEFRILGHSMCLKRRRD ESS SS S KR SCE D++ RR
Sbjct: 61  HRDEVGDVEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120

Query: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQTLR ADPNLFGIMEKEK RQ KGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIETLCRERALAAFGLDSD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFES PYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDY RFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPR+RAMLLYQGNE +QYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQ+AT EYKAYMQQVKKNAQALASALLRK Y+LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540
           GKN+E+VCE+C ITLNKITI+GDNGII PGGVRIGTPAMTSRGCLESDFE IV+FLH AA
Sbjct: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587
           QIAI VQREHGKLPKA+L+GLQ+NNEVVEL  RV SFSA+FAMPG E
Sbjct: 541 QIAIVVQREHGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLE 587

[Close]

BLAST of MC10g0444 vs. ExPASy TrEMBL
Match: A0A6J1D7A8 (Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111017683 PE=3 SV=1)

HSP 1 Score: 1192 bits (3083), Expect = 0.0
Identity = 587/587 (100.00%), Postives = 587/587 (100.00%), Query Frame = 0


Query: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60
           MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES
Sbjct: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60

Query: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120
           HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR
Sbjct: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120

Query: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT
Sbjct: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540
           GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA
Sbjct: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587
           QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE
Sbjct: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587

[Close]

BLAST of MC10g0444 vs. ExPASy TrEMBL
Match: A0A6J1FV71 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447145 PE=3 SV=1)

HSP 1 Score: 1124 bits (2907), Expect = 0.0
Identity = 551/587 (93.87%), Postives = 570/587 (97.10%), Query Frame = 0


Query: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60
           MDLS SHSNLSLGFSSHASPPHRAQIADDSVLLHLDS+RRE  YSS PVPLQLME QTES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120
           HR++ G+AER+DD+YDDREVEEFRILGHSMCLKRRRDGESS+SSGS KR+SCEFD++ RR
Sbjct: 61  HRNDGGDAERDDDEYDDREVEEFRILGHSMCLKRRRDGESSTSSGSAKRISCEFDIETRR 120

Query: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+QTLRVADPNLFGIMEKEK RQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIETLCRERALAAFGL SDNWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQ+ATPEYKAYMQQVKKNAQALA+ALLRKKY+LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540
           GKNYE VCEIC ITLNKITI+GDNGIIIPGGVR+GTPAMTSRGCLESDFETI DFLHTAA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587
           QIAISVQRE+GKLPK YLKGL +NNEVVELRNRVESFSAQFAMPGFE
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

[Close]

BLAST of MC10g0444 vs. ExPASy TrEMBL
Match: A0A6J1IXT5 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111479489 PE=3 SV=1)

HSP 1 Score: 1118 bits (2892), Expect = 0.0
Identity = 548/587 (93.36%), Postives = 567/587 (96.59%), Query Frame = 0


Query: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60
           MDLS SHSNLSLGFSSHASPPHRAQIADDSVLLHLDS+RRE  YSS PVPLQLME QTES
Sbjct: 1   MDLSKSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSSRRESSYSSTPVPLQLMEQQTES 60

Query: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120
           HR++ G+AER+DD+YDDREVEEFRILGH MCLKRRRDGESS+SS S KR+SCEFD++ RR
Sbjct: 61  HRNDGGDAERDDDEYDDREVEEFRILGHPMCLKRRRDGESSTSSSSAKRISCEFDIETRR 120

Query: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWG+QTLRVADPNLFGIMEKEK RQFKGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGDQTLRVADPNLFGIMEKEKQRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIETLCRERALAAFGL SDNWGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLSSDNWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDYARFRQIADKCGA LMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYARFRQIADKCGAALMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQ+ATPEYKAYMQQVKKNAQALA+ALLRKKY+LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQVATPEYKAYMQQVKKNAQALAAALLRKKYKLVTGGTDNHMILWDLRSLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540
           GKNYE VCEIC ITLNKITI+GDNGIIIPGGVR+GTPAMTSRGCLESDFETI DFLHTAA
Sbjct: 481 GKNYENVCEICHITLNKITIFGDNGIIIPGGVRVGTPAMTSRGCLESDFETIADFLHTAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587
           QIAISVQRE+GKLPK YLKGL +NNEVVELRNRVESFSAQFAMPGFE
Sbjct: 541 QIAISVQREYGKLPKTYLKGLLSNNEVVELRNRVESFSAQFAMPGFE 587

[Close]

BLAST of MC10g0444 vs. ExPASy TrEMBL
Match: A0A6J1E9K3 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111430628 PE=3 SV=1)

HSP 1 Score: 1076 bits (2782), Expect = 0.0
Identity = 528/587 (89.95%), Postives = 555/587 (94.55%), Query Frame = 0


Query: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60
           MDLSNSHSNLSLG SSHASPPHR QIADDS+LLHL+S+R+EP +SSPP+PLQLMEP TES
Sbjct: 1   MDLSNSHSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60

Query: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120
           HRDE G+AEREDD YDDR+VEEFRILGHSMCLKRRRD ESS SS S+KR SCE D++ RR
Sbjct: 61  HRDEVGDAEREDDVYDDRDVEEFRILGHSMCLKRRRDSESSLSSSSSKRNSCERDLETRR 120

Query: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQTLR  DPNLFGIMEKEK RQ KGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRATDPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIETLCRERALAAFGLDSD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFES PYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDY RFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYGRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGSKPR+RAMLLYQGNE +QYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSKPRRRAMLLYQGNEANQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQ+AT EYKAYMQQVKKNAQALASALLRK Y+LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540
           GKN+E+VCE+C ITLNKITI+GDNGII PGGVRIGTPAMTSRGCLESDFE IV+FLH AA
Sbjct: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587
           QIAI VQRE+GKLPKA+L+GLQ+NNEVVEL  RV SFSA+FAMPG E
Sbjct: 541 QIAIVVQRENGKLPKAFLRGLQSNNEVVELGYRVASFSAKFAMPGLE 587

[Close]

BLAST of MC10g0444 vs. ExPASy TrEMBL
Match: A0A6J1JAX6 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111482831 PE=3 SV=1)

HSP 1 Score: 1053 bits (2723), Expect = 0.0
Identity = 520/587 (88.59%), Postives = 548/587 (93.36%), Query Frame = 0


Query: 1   MDLSNSHSNLSLGFSSHASPPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTES 60
           MDLSNS+SNLSLG SSHASPPHR QIADDS+LLHL+S+R+EP +SSPP+PLQLMEP TES
Sbjct: 1   MDLSNSNSNLSLGISSHASPPHRVQIADDSILLHLESSRQEPTFSSPPIPLQLMEPLTES 60

Query: 61  HRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSSSSGSTKRVSCEFDMDARR 120
           HRDE G AEREDD    R+VEEF ILGHSMCLKRRRD ESS SS S KR SCE D++ RR
Sbjct: 61  HRDEVGGAEREDD----RDVEEFHILGHSMCLKRRRDSESSFSSSSAKRNSCERDLETRR 120

Query: 121 SAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSE 180
           SAVRSWGNQTLR ADPNLFGIMEKEK RQ KGIELIASENYVCRAVMEALGSHLTNKYSE
Sbjct: 121 SAVRSWGNQTLRAADPNLFGIMEKEKQRQIKGIELIASENYVCRAVMEALGSHLTNKYSE 180

Query: 181 GMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPG 240
           GMPGARYY GNQYIDEIETLCRERALAAFGLDSD+WGVNVQPYSCTSANFAVYTGLLLPG
Sbjct: 181 GMPGARYYCGNQYIDEIETLCRERALAAFGLDSDSWGVNVQPYSCTSANFAVYTGLLLPG 240

Query: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFR 300
           DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFES PYKVNPQTGYIDYDKLEERALDFR
Sbjct: 241 DRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESFPYKVNPQTGYIDYDKLEERALDFR 300

Query: 301 PKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360
           PKILICGGSSYPRELDY RFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST
Sbjct: 301 PKILICGGSSYPRELDYRRFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTST 360

Query: 361 THKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHI 420
           THKSLRGPRGGIIFYRKGS+PR  AMLLYQGNE++QYDFEEKINFAVFPSLQGGPHNNHI
Sbjct: 361 THKSLRGPRGGIIFYRKGSRPRTIAMLLYQGNESNQYDFEEKINFAVFPSLQGGPHNNHI 420

Query: 421 AALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLT 480
           AALAVALKQ+AT EYKAYMQQVKKNAQALASALLRK Y+LVTGGTDNHM+LWDLR LGLT
Sbjct: 421 AALAVALKQVATLEYKAYMQQVKKNAQALASALLRKNYKLVTGGTDNHMVLWDLRSLGLT 480

Query: 481 GKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAA 540
           GKN+E+VCE+C ITLNKITI+GDNGII PGGVRIGTPAMTSRGCLESDFE IV+FLH AA
Sbjct: 481 GKNFEQVCEMCHITLNKITIFGDNGIITPGGVRIGTPAMTSRGCLESDFEVIVEFLHIAA 540

Query: 541 QIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 587
           QIA+ VQREHGKLPK +L+GLQ++NEVVEL  RV SFSA+FAMPG E
Sbjct: 541 QIAVVVQREHGKLPKPFLRGLQSSNEVVELGYRVASFSAKFAMPGLE 583

[Close]

BLAST of MC10g0444 vs. TAIR 10
Match: AT1G36370.1 (serine hydroxymethyltransferase 7 )

HSP 1 Score: 827.0 bits (2135), Expect = 9.4e-240
Identity = 411/591 (69.54%), Postives = 478/591 (80.88%), Query Frame = 0


Query: 1   MDLSNSHSNLSLGFS-SHAS----PPHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLME 60
           MDLS S +N  LGF  SHAS    P  RA IADDS+ L +D + R  P +  P+PLQL+E
Sbjct: 1   MDLSRSQTNFQLGFGCSHASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLE 60

Query: 61  PQTESHRDEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGE-SSSSSGSTKRVSC-E 120
            + E     D   +      D +E E FRILGH MCLKR+RD     + S   KR S  +
Sbjct: 61  QKAEKTTTVDEPKKDGGGGGDQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPKRSSIGD 120

Query: 121 FDMDARRSAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSH 180
            D+++RR+AVR+WG+Q + +ADP++  +MEKEK RQ +GIELIASEN+VCRAVMEALGSH
Sbjct: 121 SDLESRRAAVRAWGDQPIHLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSH 180

Query: 181 LTNKYSEGMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVY 240
           LTNKYSEGMPGARYY GNQYID+IE LC ERAL AFGL+SD WGVNVQPYSCTSANFAVY
Sbjct: 181 LTNKYSEGMPGARYYTGNQYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVY 240

Query: 241 TGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLE 300
           TGLLLPG+RIMGLD+PSGG+ SHGYCTP G+K+S ASIFFES PYKVNPQTGYIDYDKLE
Sbjct: 241 TGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLE 300

Query: 301 ERALDFRPKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGY 360
           ++ALD+RPKILICGGSSYPR+ D+AR RQIADKCGAVLMCDMA ISGLVA KEC +PF +
Sbjct: 301 DKALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDH 360

Query: 361 CDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQG 420
           CD+VTSTTHK LRGPRGGIIFYR+G K RK+       + +  YD EEKINFAVFPSLQG
Sbjct: 361 CDIVTSTTHKGLRGPRGGIIFYRRGPKIRKQGHHSSHCDTSTHYDLEEKINFAVFPSLQG 420

Query: 421 GPHNNHIAALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWD 480
           GPHNNHIAALA+ALKQ+ATPEYKAY+QQ+KKNAQALA+ALLR+K RLVTGGTDNH+LLWD
Sbjct: 421 GPHNNHIAALAIALKQVATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWD 480

Query: 481 LRPLGLTGKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIV 540
           L P+GLTGK YEKVCE+C ITLNK  I+GDNG I PGGVRIGTPAMT+RGC+ESDFET+ 
Sbjct: 481 LTPMGLTGKVYEKVCEMCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMA 540

Query: 541 DFLHTAAQIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMP 585
           DFL  AAQI  ++QREHGK  K ++K L  N ++ ELRNRVE+F+ Q+ MP
Sbjct: 541 DFLIKAAQITSALQREHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

[Close]

BLAST of MC10g0444 vs. TAIR 10
Match: AT1G22020.1 (serine hydroxymethyltransferase 6 )

HSP 1 Score: 818.9 bits (2114), Expect = 2.6e-237
Identity = 406/594 (68.35%), Postives = 484/594 (81.48%), Query Frame = 0


Query: 6   SHSNLSLGF-SSHASP---PHRAQIADDSVLLHLDSTRREPPYSSPPVPLQLMEPQTESH 65
           + S+LSLGF SSHA P   P R  IADDS+ L +DS+ R      PPVPLQL+E + +  
Sbjct: 5   AQSDLSLGFGSSHALPLPHPPRIPIADDSITLQIDSSFRPSSNPMPPVPLQLLEQRFDVT 64

Query: 66  R------DEDGEAEREDDDYDDREVEEFRILGHSMCLKRRRDGESSS--SSGSTKRVSCE 125
                  +ED E   ++DD D RE E+F +LGH M LKR R G S S  SS   KR   +
Sbjct: 65  GSCSRVVEEDDEVVGDNDDDDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFVVD 124

Query: 126 FDMDARRSAVRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSH 185
             +++RR+ VR+WGNQ++  ADP +   MEKEK RQF+GIELIASEN+VCRAVMEALGSH
Sbjct: 125 SGIESRRAVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSH 184

Query: 186 LTNKYSEGMPGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVY 245
           LTNKYSEGMPGARYY GNQYID+IE LC+ERALAAFGL+ + WGVNVQPYSCTSANFAV+
Sbjct: 185 LTNKYSEGMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVF 244

Query: 246 TGLLLPGDRIMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLE 305
           TGLL+PG+RIMGLD+PSGG+ SHGY TP G+KVSGASIFFES PYKV+P+TGYIDYDKLE
Sbjct: 245 TGLLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLE 304

Query: 306 ERALDFRPKILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGY 365
           E+ALD+RPKILICGGSSYPR+ ++ RFR IADKCGAVLM DMAQISGLVAAKE  +PF Y
Sbjct: 305 EKALDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDY 364

Query: 366 CDVVTSTTHKSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQG 425
           CD+VTSTTHKSLRGPRGGIIFY++G KP+K+++ L       QYDFEEKINF+VFPSLQG
Sbjct: 365 CDIVTSTTHKSLRGPRGGIIFYKRGLKPKKQSINLNHCESNIQYDFEEKINFSVFPSLQG 424

Query: 426 GPHNNHIAALAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWD 485
           GPHNNHIAALA+ALKQ A+PEYK YM+QVKKNA+ALASAL+ +K +L+TGGTDNH+LLWD
Sbjct: 425 GPHNNHIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWD 484

Query: 486 LRPLGLTGKNYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIV 545
           L PLGLTGK YEKVCE+C IT+NK+ I+ +NG+I PGGVRIG+PAMTSRGCLE +FET+ 
Sbjct: 485 LTPLGLTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMA 544

Query: 546 DFLHTAAQIAISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGFE 588
           DFL+ AAQIA + QREHGKL K  LK + +  E+ +LRN+VE+F+ QFAMP F+
Sbjct: 545 DFLYRAAQIASAAQREHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFD 598

[Close]

BLAST of MC10g0444 vs. TAIR 10
Match: AT4G13930.1 (serine hydroxymethyltransferase 4 )

HSP 1 Score: 626.3 bits (1614), Expect = 2.4e-179
Identity = 300/464 (64.66%), Postives = 359/464 (77.37%), Query Frame = 0


Query: 123 VRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V SWGN +L   DP +  ++EKEK RQ +GIELIASEN+   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 183 PGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYYGGN++IDEIE LCR RAL AF  D   WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPK 302
           IMGLD PSGG+ +HGY T  G+K+S  SI+FESLPYKVN  TGYIDYDKLEE+ALDFRPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 303 ILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 362
           +LICGGS+YPR+ DYARFR IADK GA+L+CDMA ISGLVAA+E  +PF YCDVVT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP K+        E   YDFE+KINFAVFP+LQGGPHN+ I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKGQ-----PEGAVYDFEDKINFAVFPALQGGPHNHQIGA 303

Query: 423 LAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLTGK 482
           LAVALKQ  TP +K Y +QVK NA AL + L+ K Y++VT GT+NH++LWDLRPLGLTG 
Sbjct: 304 LAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLTGN 363

Query: 483 NYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAAQI 542
             EK+C++C ITLNK  ++GD+  + PGGVRIG PAMTSRG +E DFE I +FL  A  +
Sbjct: 364 KVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAVTL 423

Query: 543 AISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGF 587
            + +Q+ +GKL K + KGL NN ++ +L+  VE FSA + MPGF
Sbjct: 424 TLDIQKTYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGF 462

[Close]

BLAST of MC10g0444 vs. TAIR 10
Match: AT4G13890.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 621.3 bits (1601), Expect = 7.9e-178
Identity = 293/464 (63.15%), Postives = 354/464 (76.29%), Query Frame = 0


Query: 123 VRSWGNQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGM 182
           V SWGN  L   DP ++ ++EKEKHRQ +GIELIA+EN+   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 183 PGARYYGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPGDR 242
           PG RYYGG ++IDEIE+LCR R+L AF  + + WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 243 IMGLDTPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPK 302
           IMGLD PSGG+ +HGY +  G+ +S  SI+FE+LPYKV+ +TGYIDYDKLEE+A+DFRPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 303 ILICGGSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTH 362
           ++ICGG+SYPRE DYARFR +ADK GA L+CDMA  S LVAA+E   PF YCDVVT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 363 KSLRGPRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAA 422
           KSLRGPR G+IFYRKG KP K+        E + YDF+ KIN AVFP+LQ GPHNN I A
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKGQ-----PEGEVYDFDAKINSAVFPALQSGPHNNKIGA 303

Query: 423 LAVALKQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLTGK 482
           LAVALKQ+  P +K Y +QVK NA  LAS L+ K Y LVT GTDNH++LWDLRPLGLTG 
Sbjct: 304 LAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLTGN 363

Query: 483 NYEKVCEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAAQI 542
             EKVCE+C ITLN+  ++GD   + PGGVRIGTPAMTSRG +E DFE I +FLH A  I
Sbjct: 364 KVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAVTI 423

Query: 543 AISVQREHGKLPKAYLKGLQNNNEVVELRNRVESFSAQFAMPGF 587
            + +Q ++GK+ K + KGL NN E+ E++  VE F+  F MPGF
Sbjct: 424 TLDIQEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGF 462

[Close]

BLAST of MC10g0444 vs. TAIR 10
Match: AT4G37930.1 (serine transhydroxymethyltransferase 1 )

HSP 1 Score: 495.7 bits (1275), Expect = 5.0e-140
Identity = 249/465 (53.55%), Postives = 324/465 (69.68%), Query Frame = 0


Query: 128 NQTLRVADPNLFGIMEKEKHRQFKGIELIASENYVCRAVMEALGSHLTNKYSEGMPGARY 187
           N  L   DP +  I+E EK RQ+KG+ELI SEN+   +VM+A+GS +TNKYSEG PGARY
Sbjct: 52  NAPLEEVDPEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARY 111

Query: 188 YGGNQYIDEIETLCRERALAAFGLDSDNWGVNVQPYSCTSANFAVYTGLLLPGDRIMGLD 247
           YGGN+YID  ETLC++RAL AF LD + WGVNVQP S + ANF VYT LL P +RIM LD
Sbjct: 112 YGGNEYIDMAETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALD 171

Query: 248 TPSGGNTSHGYCTPNGRKVSGASIFFESLPYKVNPQTGYIDYDKLEERALDFRPKILICG 307
            P GG+ SHGY T + +K+S  SIFFE++PY+++  TGYIDYD++E+ A  FRPK+++ G
Sbjct: 172 LPHGGHLSHGYQT-DTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAG 231

Query: 308 GSSYPRELDYARFRQIADKCGAVLMCDMAQISGLVAAKECVSPFGYCDVVTSTTHKSLRG 367
            S+Y R  DYAR R++ +K  AV++ DMA ISGLVAA    SPF Y DVVT+TTHKSLRG
Sbjct: 232 ASAYARLYDYARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRG 291

Query: 368 PRGGIIFYRKGSKPRKRAMLLYQGNETDQYDFEEKINFAVFPSLQGGPHNNHIAALAVAL 427
           PRG +IF+RKG K   +     QG E   YDFE+KIN AVFP LQGGPHN+ I  LAVAL
Sbjct: 292 PRGAMIFFRKGVKEINK-----QGKEV-LYDFEDKINQAVFPGLQGGPHNHTITGLAVAL 351

Query: 428 KQLATPEYKAYMQQVKKNAQALASALLRKKYRLVTGGTDNHMLLWDLRPLGLTGKNYEKV 487
           KQ  T EYKAY +QV  N+   A  L+ + Y LV+GGTDNH++L +L+P G+ G   EKV
Sbjct: 352 KQATTSEYKAYQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKV 411

Query: 488 CEICQITLNKITIYGDNGIIIPGGVRIGTPAMTSRGCLESDFETIVDFLHTAAQIAISVQ 547
            E   I  NK T+ GD   ++PGG+R+GTPA+TSRG +E DF  + ++   A  IA+ V+
Sbjct: 412 LEAVHIASNKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVK 471

Query: 548 RE-HGKLPKAYLKGLQNN----NEVVELRNRVESFSAQFAMPGFE 588
            E  G   K ++  ++++    +E+ +LR+ VE F+ QF   GFE
Sbjct: 472 SEAQGTKLKDFVSAMESSSTIQSEIAKLRHEVEEFAKQFPTIGFE 509

The following BLAST results are available for this feature:

BLAST of MC10g0444 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84WV0	1.3e-238	69.54	Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 S...	[more]
Q9LM59	3.6e-236	68.35	Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 S...	[more]
O23254	3.4e-178	64.66	Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 S...	[more]
Q9SVM4	1.1e-176	63.15	Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 S...	[more]
Q54Z26	6.3e-156	58.39	Serine hydroxymethyltransferase 1 OS=Dictyostelium discoideum OX=44689 GN=shmt1 ...	[more]

BLAST of MC10g0444 vs. NCBI nr


Match Name	Identity	Description
XP_022149204.1	100.00	serine hydroxymethyltransferase 7-like [Momordica charantia] >XP_022149205.1 ser...	[more]
XP_022941930.1	93.87	serine hydroxymethyltransferase 7-like [Cucurbita moschata] >KAG6600365.1 Serine...	[more]
XP_023547556.1	93.53	serine hydroxymethyltransferase 7-like [Cucurbita pepo subsp. pepo]	[more]
XP_022979958.1	93.36	serine hydroxymethyltransferase 7-like [Cucurbita maxima]	[more]
KAG6576983.1	90.12	Serine hydroxymethyltransferase 7, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of MC10g0444 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1D7A8	100.00	Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111017683 P...	[more]
A0A6J1FV71	93.87	Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447145 PE...	[more]
A0A6J1IXT5	93.36	Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111479489 PE=3...	[more]
A0A6J1E9K3	89.95	Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111430628 PE...	[more]
A0A6J1JAX6	88.59	Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111482831 PE=3...	[more]

BLAST of MC10g0444 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G36370.1	9.4e-240	69.54	serine hydroxymethyltransferase 7	[more]
AT1G22020.1	2.6e-237	68.35	serine hydroxymethyltransferase 6	[more]
AT4G13930.1	2.4e-179	64.66	serine hydroxymethyltransferase 4	[more]
AT4G13890.1	7.9e-178	63.15	Pyridoxal phosphate (PLP)-dependent transferases superfamily protein	[more]
AT4G37930.1	5.0e-140	53.55	serine transhydroxymethyltransferase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015422	Pyridoxal phosphate-dependent transferase, small domain	GENE3D	3.90.1150.10	Aspartate Aminotransferase, domain 1	coord: 135..584 e-value: 6.6E-195 score: 649.6
IPR039429	Serine hydroxymethyltransferase-like domain	PFAM	PF00464	SHMT	coord: 132..535 e-value: 4.1E-171 score: 569.0
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 160..440 e-value: 6.6E-195 score: 649.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..77
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..18
None	No IPR available	PANTHER	PTHR11680:SF45	SERINE HYDROXYMETHYLTRANSFERASE	coord: 74..587
IPR001085	Serine hydroxymethyltransferase	PANTHER	PTHR11680	SERINE HYDROXYMETHYLTRANSFERASE	coord: 74..587
IPR001085	Serine hydroxymethyltransferase	HAMAP	MF_00051	SHMT	coord: 130..585 score: 39.935329
IPR001085	Serine hydroxymethyltransferase	CDD	cd00378	SHMT	coord: 135..577 e-value: 0.0 score: 576.076
IPR019798	Serine hydroxymethyltransferase, pyridoxal phosphate binding site	PROSITE	PS00096	SHMT	coord: 355..371
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 122..586

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g0444.1	MC10g0444.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046655	folic acid metabolic process
biological_process	GO:0019264	glycine biosynthetic process from serine
biological_process	GO:0006565	L-serine catabolic process
biological_process	GO:0032259	methylation
biological_process	GO:0035999	tetrahydrofolate interconversion
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004372	glycine hydroxymethyltransferase activity
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0070905	serine binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003824	catalytic activity