MC10g0420 (gene) Bitter gourd (Dali-11) v1

BLAST of MC10g0420 vs. ExPASy Swiss-Prot
Match: Q5IBC5 (Separase OS=Arabidopsis thaliana OX=3702 GN=ESP1 PE=2 SV=1)

HSP 1 Score: 1682.5 bits (4356), Expect = 0.0e+00
Identity = 970/2226 (43.58%), Postives = 1339/2226 (60.15%), Query Frame = 0

Query: 2    ASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKRF 61
            +S  +  L+S+++  D+  + S  SD+L+PFS          S K  D ++ IR+LAK+F
Sbjct: 3    SSGDDLRLLSLIDVGDN--VFSSFSDYLKPFSTLST------SRKKQDRATTIRALAKQF 62

Query: 62   LTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQR 121
            L FLN+++S+LPKRL+         + AL+ F+ Y+LCL CLE + +QL+CKP+TV  QR
Sbjct: 63   LPFLNKSISLLPKRLSVANSDKEARESALDLFRAYELCLDCLELVSAQLACKPHTVQSQR 122

Query: 122  VRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLLL 181
            +RM+HC++ WGLY++   E F++LE LR    +S+  +L     P+V    GD +  L++
Sbjct: 123  LRMIHCLDVWGLYENVYTEAFKVLEKLRGSDSKSRKSRL----LPEVQ--DGDAEMALVV 182

Query: 182  VEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCTI 241
            V+ V  I +  A  +  D   Y +VL L+EEV  W R+LD KV EK HRA+VT + KC +
Sbjct: 183  VDAVAAIFRAVAMSQQLDDKRYRKVLLLLEEVGGWLRVLDAKVYEKLHRAMVTSMGKCAV 242

Query: 242  FLVEELACFGGSLTSLFCRTTLAEYAKSSL-RDQIYKVARRICSTLF---SQHLKEDSSM 301
             LV E   F G L   FC  T+ E+ KS+L +D++YK AR + S LF    + +     +
Sbjct: 243  SLVREAERFNGDLVISFCDLTVKEHYKSALSKDRVYKFAREVLSVLFGFKDRKMSVTIDI 302

Query: 302  LICVLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQAT 361
             + VL+S++ + ++E     +EF  L+ Y A+K ++A    C   +K L +MA    +A 
Sbjct: 303  SMSVLRSLSCQFEDESNENLMEFFDLVDYCAHKFRAAGDMYCAKVSKKLNEMAAIFVEAI 362

Query: 362  TPLGMILRLYAAGLKIF---SKLPRGETHDSA----FSILVDDGESMQGLANLNGYLGSY 421
              L ++LRLY+ GL I    SKL   +  DS        + DD    Q L +L G + SY
Sbjct: 363  PQLNLVLRLYSTGLSITVCNSKLGEIKLEDSTDDWKIQAMFDDDARWQSLVSLLGMVDSY 422

Query: 422  FRIGCREGNESCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLA 481
                  EGN++ S           H N N++   +   R + T  P Y+DA+KFLCQPLA
Sbjct: 423  ---SGDEGNQTGSSSIG------GHRNYNNKTHDSCKDRNKITCWPQYVDALKFLCQPLA 482

Query: 482  ELVNSERKKILAEDE---AASVLYNIQNTLHQYCDVFLFYPPSLSCFRRAYDAERNGCDE 541
            + + S ++KI+ E E   A++ L  I +   Q+CD  LF        +R    + +    
Sbjct: 483  DFIYSVKRKIVLETEMSCASAHLITIHDAFLQFCDGCLF-------LQRCTSDKGDREIA 542

Query: 542  NVNMLLSIVVAAFTLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYR 601
            N    L+  + AF +S RT+L ++ S +L++++I S W+Q   LK+L A+L NIGI+LYR
Sbjct: 543  NNKAFLNAAMGAFIVSLRTQLKLEISAHLVEDVIGSPWIQSQELKYLIATLYNIGIVLYR 602

Query: 602  HKQIKEASKALKLCCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLV 661
            +K++ +A +ALKLC + SW CV   C  F ++S  SDN+ SEDA++ FV E   R AF +
Sbjct: 603  NKELNKACEALKLCSKVSWRCVELHCHMFVNQSSSSDNDLSEDAIMDFVGEACNRCAFYL 662

Query: 662  DILYQCDIYKIEKTMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTP 721
            DIL +C   KI + +  ILENW  A  L   LP P  +VKQWVK++ + H + D   +  
Sbjct: 663  DILQKCSRRKIRQNIVHILENWLSAEHLIRRLPGPEAIVKQWVKIERECHTDLDAAGSCT 722

Query: 722  TLNCLLPPSGMMSKAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDH 781
            TL  LL  S   SK  IG +LEQEL+ Y+ +  L     Q  ++KI   LL+DVY+T D 
Sbjct: 723  TLYSLLSSSQKKSKRGIGKILEQELLAYDRVLPLRSNLGQQTRIKIADILLKDVYVTEDM 782

Query: 782  MLERARVLIKKARALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYC 841
             +ERAR+LI KAR  R   T  + + I  LS+AIS + E   G    G    H L +AYC
Sbjct: 783  HIERARILIWKARMTRTSGTEHITECICFLSEAISILGELHHGPNEEGSPSSHMLPIAYC 842

Query: 842  LRALCTHEAVPNSKQQVLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLL 901
            LRA CT EA PNSK +V QDI ++L++WL ILSLD   D    + +E ++ LL N  DL+
Sbjct: 843  LRAFCTQEADPNSK-KVFQDISTSLNLWLRILSLDDSGDS---LPTENIIPLLYNMIDLM 902

Query: 902  SIKGCMDYHHNIYSLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEH 961
            S+KGC + HH+IY L+ RLFKWKNV+LE  LA+LWE RR+ HA+C +P+++ FI  LSE+
Sbjct: 903  SVKGCTELHHHIYQLIFRLFKWKNVKLEVCLAMLWECRRLSHALCPSPISDAFIQTLSEN 962

Query: 962  LGELPKSFDFWTNCLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAA 1021
              +     DFW +CLK     L+GFQQNF  L +       + E   Q  +T+D+ K+AA
Sbjct: 963  CADKSTCIDFWMDCLKDSKAKLIGFQQNFHDLHN-------KDEGPFQSDITIDDIKDAA 1022

Query: 1022 SKLISHIPASKSSIFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSV 1081
            S+LIS    S +S F A YLYYDLCERLIS G L+EALS AKEA+++R+ +FQ+ F Y+ 
Sbjct: 1023 SELISSASLSGNSSFAAAYLYYDLCERLISFGKLSEALSYAKEAYRIRTLIFQDKFKYTA 1082

Query: 1082 EQHPEKYCEIAHVSQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLE 1141
            E+H EK+ E   +S+   + IKN +    +A + W      WD+  CYLSP++VLQCYLE
Sbjct: 1083 EKHIEKHNEDGKISEIRTFSIKNFQVYRLLATDFWPCGNFLWDINRCYLSPWSVLQCYLE 1142

Query: 1142 STLQVGLVHEYIGNGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQK 1201
            STLQVG+++E IGNG EAET+L WGK+ SC QSL  F VAFSSALG +Y KKQ   LA+K
Sbjct: 1143 STLQVGILNELIGNGLEAETILSWGKAFSCSQSLFPFVVAFSSALGNLYHKKQCLDLAEK 1202

Query: 1202 ELEGAKQIL--TDSITSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLY 1261
            EL+ AK+IL       SC+KC+L LEV +D+ LGD+ R      +     +  ++AE L+
Sbjct: 1203 ELQNAKEILIANQRDFSCVKCKLKLEVTLDKQLGDISRKQIDRVS---QTDGFLHAESLF 1262

Query: 1262 KLALEKLNLSVWKDSISCPDED----------------RYLSSLTTQAERPKAKRDGKKC 1321
              AL K   S WK  I    E+                   S      + P   R  ++ 
Sbjct: 1263 SAALGKFCCSAWKSCIRSHGEEIAEEIVIDRNGGEGLGHNSSKTKLSIKEPPGNRGSRRG 1322

Query: 1322 KKAINTSRSFQMDQCINPQSNVRLTRSRCRSIQSQSTSNSNDVEVDLSVHLKSNVPDRSS 1381
             +A  T  S   D    P S  RLTRS   S++ Q  + SN  EV   V  K N+ DRS 
Sbjct: 1323 GRANKTCLSKDQDLISEPTS--RLTRSMRHSLREQCQNRSNVPEV---VSKKPNLCDRSV 1382

Query: 1382 ASGQRQLHLQINSCTPTSVCGASCKNGKVGCWQCLPMEITEAGQMNNFIYLKWEFVRKRL 1441
             S   ++ L  ++  P       C   K    QCL  E+TE+G +NN + LKWE   ++L
Sbjct: 1383 GSRGERVLLDTSNALP-----GFCICYKEKRQQCLSEEVTESGSLNNLVSLKWELCHRKL 1442

Query: 1442 LLRQLSGLGKCLGIRGQFHQTHETIFKSMSIVVSRNLFFQAHYAVEPTVLLDLVGKEVHG 1501
                L  LGKCLG  G+ H  HE +  S+S++      + +H     + LL+ +GKEV  
Sbjct: 1443 ASSILVSLGKCLGDSGRIHLAHEALLHSISVLFKST--WSSHNQPSVSQLLEFIGKEVTR 1502

Query: 1502 DMFAVERASVLYNVCWFSLKSCKYNDSKIICCPLSQVQFKTLASWLMVALVLCSEVPVLL 1561
            D+FAV+RA +LYN+CW +L++     S+ ICC L  + F  L SWLM+A VL  EVP+L 
Sbjct: 1503 DVFAVDRAIILYNLCWLNLRNYHCRKSRSICCDLFHIPFTKLVSWLMLAFVLSGEVPILF 1562

Query: 1562 QKVSKLLAAVHVISSSRELFSLPSSNQILTDSHWASYFHQASIGTHLNYQY---FPYRTG 1621
            QKVS+LLA+++++SSS   F+  S    L+ SHW S+FHQAS+GTHL+Y +      +  
Sbjct: 1563 QKVSRLLASLYLLSSSNSEFTFESDGNELSASHWVSFFHQASLGTHLSYHFISNLSQKHK 1622

Query: 1622 SSCVQDLNFAKGFDTG----EQTLKSRRGPISSQDLEEFARKFFDGLPCMTIVCISLIGG 1681
            S C+ D    +   +     E     R  P  +QDL +FA++FF  LP  TI+CISL+GG
Sbjct: 1623 SQCLSDKECTEATCSSCMVPEDLDLPRLAPDRTQDLVQFAKEFFINLPSSTIICISLLGG 1682

Query: 1682 DLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPVETI---LKEDYSNPQSDDIYER 1741
             L  LLQ+ +   S V  W+L+S LN + QP+  LLPV++I   + +D +N  S +  + 
Sbjct: 1683 ALNQLLQELMHIRSPVCAWVLISRLNPESQPVATLLPVDSIVEDMSDDSANLSSTEATQV 1742

Query: 1742 NDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTEDTKANRMLWWKRRTKLDDCL 1801
              L   W CPWG++V+DEVAPAF+ IL+E++ SSS    EDT  +R LWWK+R KL+  L
Sbjct: 1743 KSLKGPWLCPWGTTVVDEVAPAFKSILEESHSSSST-TEEDTIESRGLWWKKRKKLNHRL 1802

Query: 1802 GKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLRSKCKMDVNESLLKVILEGP 1861
            G  L  +E SWLGPW+ +LLGE +N K  DS    LV DL+SKCKM+VNE LLKVIL G 
Sbjct: 1803 GIFLRNLEASWLGPWRCLLLGEWSNYKLPDSAQKKLVNDLKSKCKMEVNEMLLKVILGGG 1862

Query: 1862 EEVL--EAFGSNLNSRKGCFVGKVG-LHDKQRSDPFQNTFNGVDQLSALALKLIQDAKKE 1921
             +    EA  + L+ R GC+VG+ G L+++          N  +    LALKLI DA  +
Sbjct: 1863 TDNFKGEACVAQLSLRNGCYVGRGGYLYEEDSCKTPTAASNISESRHELALKLIHDAASK 1922

Query: 1922 LEGED-NSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGSICATLDRRCRQQEQV 1981
            L  +D + +REPII VLD +VQMLPWENIPIL+ QEVYRMPSVG I A L +R  Q E  
Sbjct: 1923 LGQQDGHENREPIILVLDPEVQMLPWENIPILRKQEVYRMPSVGCISAVLKKRSLQGEPA 1982

Query: 1982 SGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKAGYAPTSAELIEELKS 2041
               + +FP IDP DSFYLLNP GDL +TQ+ FE+WF+DQN EGKAG  P++ EL E L++
Sbjct: 1983 KSHVASFPLIDPLDSFYLLNPGGDLTDTQVTFESWFRDQNFEGKAGSEPSAIELTEALET 2042

Query: 2042 RDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGYYVPQGVPLSYLKAGS 2101
             DLF+YFGHGSGAQYIPR EI+KLD C+A+ LMGCSSGSL L G Y+PQGVPLSYL  GS
Sbjct: 2043 HDLFLYFGHGSGAQYIPRREIEKLDNCSATFLMGCSSGSLWLKGCYIPQGVPLSYLLGGS 2102

Query: 2102 PVIVANLWEVTDKDIDRFGKAVLEAWLRER--SSVLPSSPRCDILTKEFETINISSKRIS 2161
            P IVA LW+VTD+DIDRFGKA+LEAWL+ER  SS      +C+ L  +   + +   + S
Sbjct: 2103 PAIVATLWDVTDRDIDRFGKALLEAWLQERSDSSSEGGCSQCESLANDLAAMTLKGTKRS 2162

Query: 2162 KKVASKNPPAACESDSSSR--GRPVTSRMIGSFLYEAREACNLRYLIGASPVCYGVPTSI 2178
            +K +S+N PA  + D S +        R IGSF+  AR+ACNL+YLIGA+PVCYGVPT I
Sbjct: 2163 RKPSSRNKPAQSDVDGSGKIECNHKHRRKIGSFIAAARDACNLQYLIGAAPVCYGVPTGI 2171

BLAST of MC10g0420 vs. ExPASy Swiss-Prot
Match: P33144 (Separin OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=bimB PE=4 SV=2)

HSP 1 Score: 204.1 bits (518), Expect = 1.6e-50
Identity = 149/448 (33.26%), Postives = 213/448 (47.54%), Query Frame = 0

Query: 1748 WWKRRTKLDDCLGKLLGTIEDSWLGPWKHML----LGELTNRKHADSVLNTLVLDLRSKC 1807
            WWK R  LD  +  LL  IE+ W G ++ +     L E +  + A +  N L   L S+ 
Sbjct: 1679 WWKNREALDRRMENLLQNIENVWFGGFRGIFSPIPLCEKSLARFASAFENILENHLPSRR 1738

Query: 1808 KMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSD------------- 1867
            K            +GP+  L      L      FVG  GL D++  +             
Sbjct: 1739 KGS--------RAQGPKLTLHPNVLEL------FVGVKGLDDQEDPEDTLMDLLYFVVDI 1798

Query: 1868 -PFQNTFNGVDQL--SALALKLIQDAKKELE-GEDNSSREP--IIFVLDYDVQMLPWENI 1927
              FQ   N  D++    + ++ +   +   E  +D +++ P   + VLD  + + PWE++
Sbjct: 1799 LQFQGERNAYDEVDFDMMVVETLDAVRAYHEAAKDQATQRPNNTVLVLDKSLHLFPWESL 1858

Query: 1928 PILQNQEVYRMPSVGSICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQ 1987
            P LQ   V R+PS+  +      R R     SG   A  SID  +  Y+LNP+GDL+ TQ
Sbjct: 1859 PCLQGLPVCRVPSLECL------RDRVLHLRSGKQSAL-SIDRRNGTYILNPTGDLKTTQ 1918

Query: 1988 IEFENWFKD-QNLEGKAGYA---PTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLD 2047
              FE   KD  +L+G  G     PT  E  + L+S+ LF+YFGHGSGAQYI    +++LD
Sbjct: 1919 ETFE---KDLSSLKGWTGMVNRQPTEDEFKDSLQSKSLFLYFGHGSGAQYIRGRTVKRLD 1978

Query: 2048 VCAASLLMGCSSGSLTLNGYYVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEA 2107
             CA + LMGCSSG+LT  G Y P G P++YL+AGSP +VA LW+VTDKDIDRF KA  E 
Sbjct: 1979 RCAVAFLMGCSSGTLTEAGEYEPYGTPMNYLQAGSPALVATLWDVTDKDIDRFAKATFEH 2038

Query: 2108 WLRERSSVLPSSPRCDILTKEFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRM 2167
            W      ++ +  R                                    + G       
Sbjct: 2039 W-----GLIGNGHR-----------------------------------GNEGIGEAGVA 2062

Query: 2168 IGSFLYEAREACNLRYLIGASPVCYGVP 2169
            + + + ++R AC L+YL GA+PV YGVP
Sbjct: 2099 LDAAVSQSRGACVLKYLNGAAPVVYGVP 2062

BLAST of MC10g0420 vs. ExPASy Swiss-Prot
Match: P18296 (Separin OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=cut1 PE=1 SV=3)

HSP 1 Score: 198.7 bits (504), Expect = 6.6e-49
Identity = 184/675 (27.26%), Postives = 279/675 (41.33%), Query Frame = 0

Query: 1518 ALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTDSHWASYFHQ--ASIGTH 1577
            ++ +C EV  L+   + + +A+  I  + ++           DS  AS+F +   ++G H
Sbjct: 1278 SVCVCREVNELISYSTIMQSALTTIGETTDV-----------DSSSASFFLEIPKALGFH 1337

Query: 1578 LNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEEFARKFFDGLPC---M 1637
               +   +R      ++L+F+    + EQ L SR   +S  D+  F   F D LP    +
Sbjct: 1338 RRREAQKFRNQH---KELHFS----SLEQILNSR---LSIPDVRTFQDNFIDSLPSIWNV 1397

Query: 1638 TIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPVETILKEDYSNPQ 1697
              + I+  G DL                   +S +   + PL+  LP    L+   S   
Sbjct: 1398 VSITINNSGEDL------------------FISKIRKGHSPLIFRLP----LQRHNSRDA 1457

Query: 1698 SDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTEDTKANRMLWWKRR 1757
             ++I                         FRII + N ++ +       + ++  WWK R
Sbjct: 1458 DEEILVFTKAQ---------------TELFRIISKSNQMAQN-GKHYTRREDKETWWKER 1517

Query: 1758 TKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLRSKCKMDVNESLL 1817
              LD CL +LL  IE SWLG +K    G     K   S+        ++    + N    
Sbjct: 1518 RHLDQCLQQLLENIEISWLGGFK----GIFNPHKIDTSLFAKFSSQFQNIIAKNFNMDKK 1577

Query: 1818 KVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQR---------SDPFQNTFNGV---- 1877
              +     E+LE F           +GK G    ++          D FQ  F G+    
Sbjct: 1578 TPVPTLSPEILELF---------ITLGKPGYEGYEQLLEDLIYFILDIFQ--FRGLHFAY 1637

Query: 1878 DQLSALALKL-IQDAKKELEGE--DNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMP 1937
            D++    L + +QDA            +R   + VLD  V   PWE++P L  Q V R+P
Sbjct: 1638 DEIDTDQLSMDLQDALNAYFNNYVSEENRSHTVLVLDKSVHQFPWESLPCLNRQSVSRVP 1697

Query: 1938 SVGSICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNL 1997
            S+  +   L     Q   V+G    +  +      Y+LNPS DL++TQ  FE+   +   
Sbjct: 1698 SLSILRDILS----QSFVVNG---EYVEVRKEAGSYILNPSLDLKHTQEMFEHKLVEGGW 1757

Query: 1998 EGKAGYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLT 2057
            +G     P++ + I+ L   D F+YFGHG G QY   +++  L  CA ++LMGCSSG+L 
Sbjct: 1758 KGLIASQPSNRDFIKMLSGNDFFLYFGHGGGEQYTTSYDLATLKRCAVTILMGCSSGALY 1817

Query: 2058 LNGYYVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCD 2117
              G + P G PL YL AG P +VANLW+VTDKDIDRF   +LE+W               
Sbjct: 1818 ECGSFEPWGTPLDYLSAGCPTLVANLWDVTDKDIDRFSLKMLESW--------------- 1826

Query: 2118 ILTKEFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRY 2172
                              K    N  + C + S                 E+R  C+LRY
Sbjct: 1878 -------------GLFENKAPFVNSTSICTAVS-----------------ESRSCCHLRY 1826

BLAST of MC10g0420 vs. ExPASy Swiss-Prot
Match: P60330 (Separin OS=Mus musculus OX=10090 GN=Espl1 PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 3.2e-43
Identity = 124/433 (28.64%), Postives = 202/433 (46.65%), Query Frame = 0

Query: 1745 RMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLRSKCK 1804
            + +WW  R  LD  +  L+  +E+  LG W+ +LL    +   A     + + +L  +C 
Sbjct: 1769 KRVWWTGRLALDQRMEALITALEEQVLGCWRGLLLPCSADPSLAQEA--SKLQELLRECG 1828

Query: 1805 MDVNES-LLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVDQLS 1864
             +  +S LLKVIL          G+ + + +       GL   Q                
Sbjct: 1829 WEYPDSTLLKVILS---------GARILTSQDVQALACGLCPAQPD-------------- 1888

Query: 1865 ALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGSICA 1924
              A  L+ +A  +++ ++    + ++ VLD D+Q LPWE+ PILQ Q V R+PS   +  
Sbjct: 1889 -RAQVLLSEAVGQVQSQEAPRSQHLVLVLDKDLQKLPWESTPILQAQPVTRLPSFRFL-- 1948

Query: 1925 TLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQ-NLEGKAGY 1984
             L     ++   S ++     +DP ++FY+LNP  +L +T+  F   F  +   +G  G 
Sbjct: 1949 -LSYTVTKEAGASSVLS--QGVDPQNTFYVLNPHSNLSSTEERFRASFSSETGWKGVIGE 2008

Query: 1985 APTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGYYV 2044
             P+  ++   L  RDL+IY GHG+GA+++    + +L   A +LL GCSS +L ++G   
Sbjct: 2009 VPSLDQVQAALTERDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLE 2068

Query: 2045 PQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTKEF 2104
              G+ L Y+ AG P+ + NLW+VTD+DIDR+ +A+L+ WL                    
Sbjct: 2069 GAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWL-------------------- 2116

Query: 2105 ETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLY---EAREACNLRYLIG 2164
                                       +  G P        FLY   +AR+A  L+YLIG
Sbjct: 2129 --------------------------GAGPGAP--------FLYYASQARQAPRLKYLIG 2116

Query: 2165 ASPVCYGVPTSIK 2173
            A+PV YG+P S++
Sbjct: 2189 AAPVAYGLPISLQ 2116

BLAST of MC10g0420 vs. ExPASy Swiss-Prot
Match: Q14674 (Separin OS=Homo sapiens OX=9606 GN=ESPL1 PE=1 SV=3)

HSP 1 Score: 177.2 bits (448), Expect = 2.0e-42
Identity = 123/428 (28.74%), Postives = 193/428 (45.09%), Query Frame = 0

Query: 1748 WWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLL--GELTNRKHADSVLNTLVLDLRSKCKM 1807
            WW  R  LD  +  L+ ++E S LG WK +LL   E        S L  L+ D   K   
Sbjct: 1776 WWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQDCGWKYP- 1835

Query: 1808 DVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVDQLSAL 1867
              + +LLK++L G                    G +   D Q        +         
Sbjct: 1836 --DRTLLKIMLSG-------------------AGALTPQDIQAL-----AYGLCPTQPER 1895

Query: 1868 ALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGSICATL 1927
            A +L+ +A   L+G    S   ++ VLD D+Q LPWE++P LQ   V R+PS   +   L
Sbjct: 1896 AQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFL---L 1955

Query: 1928 DRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFE-NWFKDQNLEGKAGYAP 1987
                 ++   S ++     +DP  +FY+LNP  +L +T+ +F  N+  +    G  G  P
Sbjct: 1956 SYSIIKEYGASPVLS--QGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVP 2015

Query: 1988 TSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGYYVPQ 2047
               ++ E L   DL+IY GHG+GA+++    + +L   A +LL GCSS +L + G     
Sbjct: 2016 RPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVRGNLEGA 2075

Query: 2048 GVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTKEFET 2107
            G+ L Y+ AG P+ + NLW+VTD+DIDR+ +A+L+ WL                      
Sbjct: 2076 GIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWL---------------------- 2120

Query: 2108 INISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGASPVC 2167
                                     +  G P+       ++ +AR+A  L+YLIGA+P+ 
Sbjct: 2136 ------------------------GAGPGAPLL-----YYVNQARQAPRLKYLIGAAPIA 2120

Query: 2168 YGVPTSIK 2173
            YG+P S++
Sbjct: 2196 YGLPVSLR 2120

BLAST of MC10g0420 vs. NCBI nr
Match: XP_022149193.1 (separase isoform X1 [Momordica charantia])

HSP 1 Score: 4337 bits (11248), Expect = 0.0
Identity = 2169/2177 (99.63%), Postives = 2169/2177 (99.63%), Query Frame = 0

Query: 1    MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR 60
            MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR
Sbjct: 1    MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR 60

Query: 61   FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ 120
            FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ
Sbjct: 61   FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL 180
            RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL
Sbjct: 121  RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL 180

Query: 181  LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT 240
            LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT
Sbjct: 181  LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT 240

Query: 241  IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC 300
            IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC
Sbjct: 241  IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC 300

Query: 301  VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL 360
            VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL
Sbjct: 301  VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL 360

Query: 361  GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE 420
            GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE
Sbjct: 361  GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE 420

Query: 421  SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI 480
            SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI
Sbjct: 421  SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI 480

Query: 481  LAEDEAASVLYNIQNTLHQYCDVFLFYPPSLSCFRRAYDAERNGCDENVNMLLSIVVAAF 540
            LAEDEAASVLYNIQNTLHQYCDVFLF      C RRAYDAERNGCDENVNMLLSIVVAAF
Sbjct: 481  LAEDEAASVLYNIQNTLHQYCDVFLF------CQRRAYDAERNGCDENVNMLLSIVVAAF 540

Query: 541  TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL 600
            TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL
Sbjct: 541  TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL 600

Query: 601  CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK 660
            CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK
Sbjct: 601  CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK 660

Query: 661  TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS 720
            TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS
Sbjct: 661  TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS 720

Query: 721  KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR 780
            KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR
Sbjct: 721  KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR 780

Query: 781  ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS 840
            ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS
Sbjct: 781  ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS 840

Query: 841  KQQVLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY 900
            KQ VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY
Sbjct: 841  KQ-VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY 900

Query: 901  SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN 960
            SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN
Sbjct: 901  SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN 960

Query: 961  CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS 1020
            CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS
Sbjct: 961  CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS 1020

Query: 1021 IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV 1080
            IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV
Sbjct: 1021 IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV 1080

Query: 1081 SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG 1140
            SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG
Sbjct: 1081 SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG 1140

Query: 1141 NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI 1200
            NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI
Sbjct: 1141 NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI 1200

Query: 1201 TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS 1260
            TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS
Sbjct: 1201 TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS 1260

Query: 1261 ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS 1320
            ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS
Sbjct: 1261 ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS 1320

Query: 1321 IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC 1380
            IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC
Sbjct: 1321 IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC 1380

Query: 1381 WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI 1440
            WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI
Sbjct: 1381 WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI 1440

Query: 1441 VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC 1500
            VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC
Sbjct: 1441 VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC 1500

Query: 1501 CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD 1560
            CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD
Sbjct: 1501 CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD 1560

Query: 1561 SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE 1620
            SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE
Sbjct: 1561 SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE 1620

Query: 1621 FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV 1680
            FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV
Sbjct: 1621 FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV 1680

Query: 1681 ETILKEDYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTED 1740
            ETILKEDYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTED
Sbjct: 1681 ETILKEDYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTED 1740

Query: 1741 TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR 1800
            TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR
Sbjct: 1741 TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR 1800

Query: 1801 SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD 1860
            SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD
Sbjct: 1801 SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD 1860

Query: 1861 QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS 1920
            QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS
Sbjct: 1861 QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS 1920

Query: 1921 ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA 1980
            ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA
Sbjct: 1921 ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA 1980

Query: 1981 GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY 2040
            GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY
Sbjct: 1981 GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY 2040

Query: 2041 YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK 2100
            YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK
Sbjct: 2041 YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK 2100

Query: 2101 EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA 2160
            EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA
Sbjct: 2101 EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA 2160

Query: 2161 SPVCYGVPTSIKKKKDV 2177
            SPVCYGVPTSIKKKKDV
Sbjct: 2161 SPVCYGVPTSIKKKKDV 2170

BLAST of MC10g0420 vs. NCBI nr
Match: XP_022149194.1 (separase isoform X2 [Momordica charantia])

HSP 1 Score: 4330 bits (11231), Expect = 0.0
Identity = 2168/2177 (99.59%), Postives = 2168/2177 (99.59%), Query Frame = 0

Query: 1    MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR 60
            MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR
Sbjct: 1    MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR 60

Query: 61   FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ 120
            FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ
Sbjct: 61   FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL 180
            RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL
Sbjct: 121  RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL 180

Query: 181  LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT 240
            LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT
Sbjct: 181  LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT 240

Query: 241  IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC 300
            IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC
Sbjct: 241  IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC 300

Query: 301  VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL 360
            VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL
Sbjct: 301  VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL 360

Query: 361  GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE 420
            GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE
Sbjct: 361  GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE 420

Query: 421  SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI 480
            SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI
Sbjct: 421  SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI 480

Query: 481  LAEDEAASVLYNIQNTLHQYCDVFLFYPPSLSCFRRAYDAERNGCDENVNMLLSIVVAAF 540
            LAEDEAASVLYNIQNTLHQYCDVFLF      C RRAYDAERNGCDENVNMLLSIVVAAF
Sbjct: 481  LAEDEAASVLYNIQNTLHQYCDVFLF------CQRRAYDAERNGCDENVNMLLSIVVAAF 540

Query: 541  TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL 600
            TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL
Sbjct: 541  TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL 600

Query: 601  CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK 660
            CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK
Sbjct: 601  CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK 660

Query: 661  TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS 720
            TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS
Sbjct: 661  TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS 720

Query: 721  KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR 780
            KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR
Sbjct: 721  KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR 780

Query: 781  ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS 840
            ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS
Sbjct: 781  ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS 840

Query: 841  KQQVLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY 900
            KQ VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY
Sbjct: 841  KQ-VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY 900

Query: 901  SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN 960
            SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN
Sbjct: 901  SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN 960

Query: 961  CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS 1020
            CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS
Sbjct: 961  CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS 1020

Query: 1021 IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV 1080
            IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV
Sbjct: 1021 IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV 1080

Query: 1081 SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG 1140
            SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG
Sbjct: 1081 SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG 1140

Query: 1141 NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI 1200
            NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI
Sbjct: 1141 NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI 1200

Query: 1201 TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS 1260
            TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS
Sbjct: 1201 TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS 1260

Query: 1261 ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS 1320
            ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS
Sbjct: 1261 ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS 1320

Query: 1321 IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC 1380
            IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC
Sbjct: 1321 IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC 1380

Query: 1381 WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI 1440
            WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI
Sbjct: 1381 WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI 1440

Query: 1441 VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC 1500
            VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC
Sbjct: 1441 VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC 1500

Query: 1501 CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD 1560
            CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD
Sbjct: 1501 CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD 1560

Query: 1561 SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE 1620
            SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE
Sbjct: 1561 SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE 1620

Query: 1621 FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV 1680
            FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV
Sbjct: 1621 FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV 1680

Query: 1681 ETILKEDYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTED 1740
            ETILK DYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTED
Sbjct: 1681 ETILK-DYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTED 1740

Query: 1741 TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR 1800
            TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR
Sbjct: 1741 TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR 1800

Query: 1801 SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD 1860
            SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD
Sbjct: 1801 SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD 1860

Query: 1861 QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS 1920
            QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS
Sbjct: 1861 QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS 1920

Query: 1921 ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA 1980
            ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA
Sbjct: 1921 ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA 1980

Query: 1981 GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY 2040
            GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY
Sbjct: 1981 GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY 2040

Query: 2041 YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK 2100
            YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK
Sbjct: 2041 YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK 2100

Query: 2101 EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA 2160
            EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA
Sbjct: 2101 EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA 2160

Query: 2161 SPVCYGVPTSIKKKKDV 2177
            SPVCYGVPTSIKKKKDV
Sbjct: 2161 SPVCYGVPTSIKKKKDV 2169

BLAST of MC10g0420 vs. NCBI nr
Match: XP_022149195.1 (separase isoform X3 [Momordica charantia])

HSP 1 Score: 4200 bits (10892), Expect = 0.0
Identity = 2116/2177 (97.20%), Postives = 2116/2177 (97.20%), Query Frame = 0

Query: 1    MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR 60
            MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR
Sbjct: 1    MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR 60

Query: 61   FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ 120
            FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ
Sbjct: 61   FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL 180
            RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL
Sbjct: 121  RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL 180

Query: 181  LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT 240
            LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT
Sbjct: 181  LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT 240

Query: 241  IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC 300
            IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC
Sbjct: 241  IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC 300

Query: 301  VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL 360
            VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL
Sbjct: 301  VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL 360

Query: 361  GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE 420
            GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE
Sbjct: 361  GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE 420

Query: 421  SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI 480
            SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI
Sbjct: 421  SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI 480

Query: 481  LAEDEAASVLYNIQNTLHQYCDVFLFYPPSLSCFRRAYDAERNGCDENVNMLLSIVVAAF 540
            LAEDEAASVLYNIQNTLHQYCDVFLF      C RRAYDAERNGCDENVNMLLSIVVAAF
Sbjct: 481  LAEDEAASVLYNIQNTLHQYCDVFLF------CQRRAYDAERNGCDENVNMLLSIVVAAF 540

Query: 541  TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL 600
            TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL
Sbjct: 541  TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL 600

Query: 601  CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK 660
            CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK
Sbjct: 601  CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK 660

Query: 661  TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS 720
            TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS
Sbjct: 661  TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS 720

Query: 721  KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR 780
            KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR
Sbjct: 721  KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR 780

Query: 781  ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS 840
            ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS
Sbjct: 781  ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS 840

Query: 841  KQQVLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY 900
            KQ VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY
Sbjct: 841  KQ-VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY 900

Query: 901  SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN 960
            SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN
Sbjct: 901  SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN 960

Query: 961  CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS 1020
            CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS
Sbjct: 961  CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS 1020

Query: 1021 IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV 1080
            IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV
Sbjct: 1021 IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV 1080

Query: 1081 SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG 1140
            SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG
Sbjct: 1081 SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG 1140

Query: 1141 NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI 1200
            NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI
Sbjct: 1141 NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI 1200

Query: 1201 TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS 1260
            TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS
Sbjct: 1201 TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS 1260

Query: 1261 ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS 1320
            ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS
Sbjct: 1261 ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS 1320

Query: 1321 IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC 1380
            IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC
Sbjct: 1321 IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC 1380

Query: 1381 WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI 1440
            WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI
Sbjct: 1381 WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI 1440

Query: 1441 VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC 1500
            VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC
Sbjct: 1441 VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC 1500

Query: 1501 CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD 1560
            CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD
Sbjct: 1501 CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD 1560

Query: 1561 SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE 1620
            SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE
Sbjct: 1561 SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE 1620

Query: 1621 FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV 1680
            FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV
Sbjct: 1621 FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV 1680

Query: 1681 ETILKEDYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTED 1740
            ETILKED                                                     
Sbjct: 1681 ETILKED----------------------------------------------------- 1740

Query: 1741 TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR 1800
            TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR
Sbjct: 1741 TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR 1800

Query: 1801 SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD 1860
            SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD
Sbjct: 1801 SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD 1860

Query: 1861 QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS 1920
            QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS
Sbjct: 1861 QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS 1920

Query: 1921 ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA 1980
            ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA
Sbjct: 1921 ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA 1980

Query: 1981 GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY 2040
            GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY
Sbjct: 1981 GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY 2040

Query: 2041 YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK 2100
            YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK
Sbjct: 2041 YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK 2100

Query: 2101 EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA 2160
            EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA
Sbjct: 2101 EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA 2117

Query: 2161 SPVCYGVPTSIKKKKDV 2177
            SPVCYGVPTSIKKKKDV
Sbjct: 2161 SPVCYGVPTSIKKKKDV 2117

BLAST of MC10g0420 vs. NCBI nr
Match: XP_022149196.1 (separase isoform X4 [Momordica charantia])

HSP 1 Score: 4107 bits (10651), Expect = 0.0
Identity = 2046/2054 (99.61%), Postives = 2046/2054 (99.61%), Query Frame = 0

Query: 124  MVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLLLVE 183
            MVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLLLVE
Sbjct: 1    MVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLLLVE 60

Query: 184  VVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCTIFL 243
            VVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCTIFL
Sbjct: 61   VVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCTIFL 120

Query: 244  VEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLICVLK 303
            VEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLICVLK
Sbjct: 121  VEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLICVLK 180

Query: 304  SITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPLGMI 363
            SITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPLGMI
Sbjct: 181  SITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPLGMI 240

Query: 364  LRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNESCS 423
            LRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNESCS
Sbjct: 241  LRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNESCS 300

Query: 424  IKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKILAE 483
            IKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKILAE
Sbjct: 301  IKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKILAE 360

Query: 484  DEAASVLYNIQNTLHQYCDVFLFYPPSLSCFRRAYDAERNGCDENVNMLLSIVVAAFTLS 543
            DEAASVLYNIQNTLHQYCDVFLF      C RRAYDAERNGCDENVNMLLSIVVAAFTLS
Sbjct: 361  DEAASVLYNIQNTLHQYCDVFLF------CQRRAYDAERNGCDENVNMLLSIVVAAFTLS 420

Query: 544  FRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKLCCR 603
            FRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKLCCR
Sbjct: 421  FRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKLCCR 480

Query: 604  ASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEKTMA 663
            ASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEKTMA
Sbjct: 481  ASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEKTMA 540

Query: 664  KILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMSKAK 723
            KILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMSKAK
Sbjct: 541  KILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMSKAK 600

Query: 724  IGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKARALR 783
            IGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKARALR
Sbjct: 601  IGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKARALR 660

Query: 784  FCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNSKQQ 843
            FCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNSKQ 
Sbjct: 661  FCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNSKQ- 720

Query: 844  VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIYSLM 903
            VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIYSLM
Sbjct: 721  VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIYSLM 780

Query: 904  IRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTNCLK 963
            IRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTNCLK
Sbjct: 781  IRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTNCLK 840

Query: 964  TLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSSIFF 1023
            TLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSSIFF
Sbjct: 841  TLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSSIFF 900

Query: 1024 AGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHVSQK 1083
            AGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHVSQK
Sbjct: 901  AGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHVSQK 960

Query: 1084 PAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIGNGS 1143
            PAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIGNGS
Sbjct: 961  PAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIGNGS 1020

Query: 1144 EAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSITSC 1203
            EAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSITSC
Sbjct: 1021 EAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSITSC 1080

Query: 1204 LKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDSISC 1263
            LKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDSISC
Sbjct: 1081 LKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDSISC 1140

Query: 1264 PDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRSIQS 1323
            PDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRSIQS
Sbjct: 1141 PDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRSIQS 1200

Query: 1324 QSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGCWQC 1383
            QSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGCWQC
Sbjct: 1201 QSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGCWQC 1260

Query: 1384 LPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSIVVS 1443
            LPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSIVVS
Sbjct: 1261 LPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSIVVS 1320

Query: 1444 RNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIICCPL 1503
            RNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIICCPL
Sbjct: 1321 RNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIICCPL 1380

Query: 1504 SQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTDSHW 1563
            SQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTDSHW
Sbjct: 1381 SQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTDSHW 1440

Query: 1564 ASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEEFAR 1623
            ASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEEFAR
Sbjct: 1441 ASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEEFAR 1500

Query: 1624 KFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPVETI 1683
            KFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPVETI
Sbjct: 1501 KFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPVETI 1560

Query: 1684 LKEDYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTEDTKA 1743
            LKEDYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTEDTKA
Sbjct: 1561 LKEDYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTEDTKA 1620

Query: 1744 NRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLRSKC 1803
            NRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLRSKC
Sbjct: 1621 NRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLRSKC 1680

Query: 1804 KMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVDQLS 1863
            KMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVDQLS
Sbjct: 1681 KMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVDQLS 1740

Query: 1864 ALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGSICA 1923
            ALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGSICA
Sbjct: 1741 ALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGSICA 1800

Query: 1924 TLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKAGYA 1983
            TLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKAGYA
Sbjct: 1801 TLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKAGYA 1860

Query: 1984 PTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGYYVP 2043
            PTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGYYVP
Sbjct: 1861 PTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGYYVP 1920

Query: 2044 QGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTKEFE 2103
            QGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTKEFE
Sbjct: 1921 QGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTKEFE 1980

Query: 2104 TINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGASPV 2163
            TINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGASPV
Sbjct: 1981 TINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGASPV 2040

Query: 2164 CYGVPTSIKKKKDV 2177
            CYGVPTSIKKKKDV
Sbjct: 2041 CYGVPTSIKKKKDV 2047

BLAST of MC10g0420 vs. NCBI nr
Match: XP_022942953.1 (separase isoform X3 [Cucurbita moschata])

HSP 1 Score: 3523 bits (9135), Expect = 0.0
Identity = 1772/2186 (81.06%), Postives = 1925/2186 (88.06%), Query Frame = 0

Query: 1    MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR 60
            MASPSE+++IS+LETADSKGI S VSDFL+PFSD KNPKK K+SAKPSDDSSAIRSLAK+
Sbjct: 1    MASPSEASIISILETADSKGIFSTVSDFLRPFSDIKNPKKSKKSAKPSDDSSAIRSLAKK 60

Query: 61   FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ 120
            FL FLNRALSILPKRL++P KLGNDL+LALEFF++Y+LCL CLESL SQLSCKPYTVDVQ
Sbjct: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEIYRLCLACLESLTSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL 180
            RVRMVHCMEDWGL+KDAE EGFRILE LR I   S     DC++  D DKGGGDK+F LL
Sbjct: 121  RVRMVHCMEDWGLFKDAEAEGFRILERLRVIDRTS-----DCRVIHDRDKGGGDKEFCLL 180

Query: 181  LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT 240
              EVVVT+VKC ASGRSK+SGDY R+LGLVEEV+PWFRLLD KVSEKT RAL TYL KCT
Sbjct: 181  FAEVVVTLVKCAASGRSKESGDYGRLLGLVEEVRPWFRLLDAKVSEKTQRALATYLGKCT 240

Query: 241  IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLI- 300
            IFLVEEL  FG SL SLFCR T AEYAKSSL+DQIYK+ARRICSTLFS   ++ SSMLI 
Sbjct: 241  IFLVEELDYFGESLVSLFCRITFAEYAKSSLKDQIYKLARRICSTLFSLQQEQHSSMLIM 300

Query: 301  ----CVLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQ 360
                CV+KS+TLE K+E     VEFLQLI YSANKC S SP+CCCAFAKHL++MAG+ HQ
Sbjct: 301  HILICVVKSLTLEWKDETEWEVVEFLQLICYSANKCLSTSPECCCAFAKHLQEMAGEFHQ 360

Query: 361  ATTPLGMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGC 420
            AT PLGM LRLYAAGLKI SKLPRGET  SAFS L+DD +++Q L NLNG+LGSYF IGC
Sbjct: 361  ATPPLGMNLRLYAAGLKIVSKLPRGETCGSAFSTLLDDEDTIQSLVNLNGFLGSYFCIGC 420

Query: 421  REGNESCSIKQKDFV-QPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVN 480
            REGNESCSI+QKDFV QPC H NSNHE  V SL   RE YL SYLDAIKF CQPLAE VN
Sbjct: 421  REGNESCSIEQKDFVGQPCLHRNSNHENGVPSLLSLREAYLSSYLDAIKFFCQPLAESVN 480

Query: 481  SERKKILAEDEAASVLYNIQNTLHQYCDVFLFYPPSLSCFRRAYDAERNGCDENVNMLLS 540
             ERK+ILAED+AA VLYNIQNTLHQ+CDVFLF      C RRAYDA+ +GCDENVNMLLS
Sbjct: 481  VERKEILAEDKAAPVLYNIQNTLHQFCDVFLF------CQRRAYDAKSDGCDENVNMLLS 540

Query: 541  IVVAAFTLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEA 600
            IVVAAFTLSFRT+L+MKRST LIK++I SKWVQP ALK LFASLNNIGIIL+R+KQI EA
Sbjct: 541  IVVAAFTLSFRTRLDMKRSTNLIKDVIASKWVQPFALKRLFASLNNIGIILFRNKQIGEA 600

Query: 601  SKALKLCCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCD 660
            SKALKLCCRASWTCV  FCE FD+KSRPSDNEFSE++VL  VNE+IMRSAFLVDILYQCD
Sbjct: 601  SKALKLCCRASWTCVKQFCEIFDNKSRPSDNEFSENSVLIIVNEVIMRSAFLVDILYQCD 660

Query: 661  IYKIEKTMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLP 720
            + K+EK M +IL NWS AAKLF+ELP+PVQLVKQWVKMQCK+HKNADPK +TPTLNCLL 
Sbjct: 661  MCKVEKAMTEILTNWSAAAKLFDELPVPVQLVKQWVKMQCKYHKNADPKKSTPTLNCLLQ 720

Query: 721  PSGMMSKAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARV 780
             S  +SKA+IG LLEQELVEYEEMS LYP FCQ LQMKILS LL D YITPDH LE+ARV
Sbjct: 721  SSETVSKARIGVLLEQELVEYEEMSGLYPEFCQSLQMKILSILLTDTYITPDHRLEKARV 780

Query: 781  LIKKARALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTH 840
            L+KKAR LR CETVCL+  IQCLS+AISTMNE SSG+ S GVLH HQLAVAYCLRA CTH
Sbjct: 781  LMKKARVLRLCETVCLDGCIQCLSEAISTMNEISSGSRSFGVLHSHQLAVAYCLRAFCTH 840

Query: 841  EAVPNSKQQVLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMD 900
            E VPNSKQ V QDIDSALS+WLEI SL++LPDDQR ILSEYMLLLLCN FDLLSIKGC+D
Sbjct: 841  ETVPNSKQ-VQQDIDSALSIWLEISSLNTLPDDQRLILSEYMLLLLCNAFDLLSIKGCVD 900

Query: 901  YHHNIYSLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKS 960
            YH NIYSLMIRLFKWKNV LEKL+A LWESRRM HAMC AP NE+ IAQLSEHLG+LPKS
Sbjct: 901  YHDNIYSLMIRLFKWKNVPLEKLMATLWESRRMSHAMCIAPANEIVIAQLSEHLGDLPKS 960

Query: 961  FDFWTNCLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHI 1020
             DFWTNCLKTLP MLVGFQQNFSFLCSNYTQ+SCEHEKS +++VTVDE KEAA KLISHI
Sbjct: 961  NDFWTNCLKTLPGMLVGFQQNFSFLCSNYTQTSCEHEKSSRMHVTVDEVKEAALKLISHI 1020

Query: 1021 PASKSSIFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKY 1080
            PA KSSIF AGYLYYDLCERLISEGCLTEAL CAKEAH+LRSKLFQE F YSVEQHPEKY
Sbjct: 1021 PACKSSIFSAGYLYYDLCERLISEGCLTEALLCAKEAHRLRSKLFQEKFTYSVEQHPEKY 1080

Query: 1081 CEIAHVSQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGL 1140
            CEI H SQKP YGIKNLRK+GSV+RN+WSFD+ISWDVE CYLSPFNVLQCYLESTLQVGL
Sbjct: 1081 CEITHASQKPPYGIKNLRKSGSVSRNIWSFDKISWDVEGCYLSPFNVLQCYLESTLQVGL 1140

Query: 1141 VHEYIGNGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQ 1200
            VHE IGNGSEAETLLQWGKSISCL+SLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQ
Sbjct: 1141 VHEIIGNGSEAETLLQWGKSISCLRSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQ 1200

Query: 1201 ILTDSITSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNL 1260
            ILTD+ITSCLKCRLVLEVIVDQ+LGDLFRSMYVN NGN SEE LINAE+LYKLALEKLNL
Sbjct: 1201 ILTDNITSCLKCRLVLEVIVDQYLGDLFRSMYVNGNGNTSEELLINAEELYKLALEKLNL 1260

Query: 1261 SVWKDSISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLT 1320
            S WK+SISCPDED +LSSLT   ER KA++DG+K KK  N  RS +MDQC+NPQ+NVRLT
Sbjct: 1261 SGWKNSISCPDEDSHLSSLTIPVERAKARKDGRKSKKTTNAPRSLKMDQCVNPQTNVRLT 1320

Query: 1321 RSRCRSIQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCK 1380
            RSRCRSIQ QST+ SN+VEVDL+VHLKSNVPD   ASGQ+Q+HLQ+N CTPT  CGASCK
Sbjct: 1321 RSRCRSIQGQSTNISNEVEVDLTVHLKSNVPDLFGASGQKQMHLQVNCCTPTLGCGASCK 1380

Query: 1381 NGKVGCWQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETI 1440
            NGKVGCWQCL MEI EAGQ+NNFIYLKWEFVR+RLLLRQLSGLGKC GIRGQ HQTHETI
Sbjct: 1381 NGKVGCWQCLQMEIMEAGQINNFIYLKWEFVRRRLLLRQLSGLGKCSGIRGQIHQTHETI 1440

Query: 1441 FKSMSIVVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYN 1500
             KSMSI+VSRN+F QAH  VEP VLLDLVGKEV GDMFAVERASVLYN+CWF+LKS KY+
Sbjct: 1441 LKSMSILVSRNIFSQAHGVVEPVVLLDLVGKEVPGDMFAVERASVLYNICWFALKSYKYD 1500

Query: 1501 DSKIICCPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSS 1560
            D+KIICCPLSQVQ KTL SWLMVALVLC EVPVL QKVSKLLA +HVISSS EL SLPSS
Sbjct: 1501 DTKIICCPLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSIELLSLPSS 1560

Query: 1561 NQILTDSHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPIS 1620
            N+ILTDSHWASYFHQASIGTHLN+Q+FPY TG SC+QDLNFAKGFDTGEQT+K  RG  S
Sbjct: 1561 NKILTDSHWASYFHQASIGTHLNHQFFPYTTGRSCIQDLNFAKGFDTGEQTVKLFRGSFS 1620

Query: 1621 SQDLEEFARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPL 1680
            SQDLEE+ RKFFDGLPC+ +VCISLIGGDLACLLQQ L +PSSVH WILVS+LNSK QPL
Sbjct: 1621 SQDLEEYVRKFFDGLPCVAMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLNSKRQPL 1680

Query: 1681 VVLLPVETILKED---YSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYL 1740
            V+LLPVETILKED   YSNPQSDDI ERND  KHW+CPWGSSVIDE+APAFR IL++NYL
Sbjct: 1681 VLLLPVETILKEDSEDYSNPQSDDICERNDSTKHWQCPWGSSVIDEIAPAFRTILEDNYL 1740

Query: 1741 SSSVFPTEDTKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSV 1800
            SSSVFP+EDTK NRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWK++LLG+ +NRKH DSV
Sbjct: 1741 SSSVFPSEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYILLGDWSNRKHVDSV 1800

Query: 1801 LNTLVLDLRSKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDP 1860
            LNTLVL+L+SKCKMDVNE LLK+ILEG E VL  F S L SRKGCFVG+   HDK+RS+P
Sbjct: 1801 LNTLVLNLKSKCKMDVNEGLLKIILEGSENVLVGFDSKLYSRKGCFVGRARFHDKERSNP 1860

Query: 1861 FQNTFNGVDQLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQE 1920
            FQN  NGVD+LS LALKLIQDAKKELEGED +SREPII VLDYDVQMLPWENIPIL+NQE
Sbjct: 1861 FQNALNGVDKLSTLALKLIQDAKKELEGEDGTSREPIILVLDYDVQMLPWENIPILKNQE 1920

Query: 1921 VYRMPSVGSICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWF 1980
            VYRMPSVGSIC TLD R  QQEQ SGII  FPSIDP D+FYLLNPSGDL +TQIEFENWF
Sbjct: 1921 VYRMPSVGSICVTLDGRLHQQEQDSGIIATFPSIDPLDAFYLLNPSGDLSSTQIEFENWF 1980

Query: 1981 KDQNLEGKAGYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCS 2040
            KDQNLEGKAGYAP S+ELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLD CAASLLMGCS
Sbjct: 1981 KDQNLEGKAGYAPKSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAASLLMGCS 2040

Query: 2041 SGSLTLNGYYVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPS 2100
            SGSLTLNGYYVPQG+PLSYLKAGSPVIVANLWEVTDKDIDRFGKA+LEAWLRERS  LPS
Sbjct: 2041 SGSLTLNGYYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRERSCALPS 2100

Query: 2101 SPRCDILTKEFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREA 2160
            S +CDI+TKE E + ISSK  +KKVASK+ P  CES SSS+   V SRMIGSFL EAREA
Sbjct: 2101 SVQCDIVTKELEAMKISSKLANKKVASKSLPTTCES-SSSKDHSVHSRMIGSFLCEAREA 2160

Query: 2161 CNLRYLIGASPVCYGVPTSIKKKKDV 2177
            CNLRYLIGASPVCYGVPTSI+KKK++
Sbjct: 2161 CNLRYLIGASPVCYGVPTSIRKKKEL 2173

BLAST of MC10g0420 vs. ExPASy TrEMBL
Match: A0A6J1D658 (Separase OS=Momordica charantia OX=3673 GN=LOC111017677 PE=4 SV=1)

HSP 1 Score: 4337 bits (11248), Expect = 0.0
Identity = 2169/2177 (99.63%), Postives = 2169/2177 (99.63%), Query Frame = 0

Query: 1    MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR 60
            MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR
Sbjct: 1    MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR 60

Query: 61   FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ 120
            FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ
Sbjct: 61   FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL 180
            RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL
Sbjct: 121  RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL 180

Query: 181  LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT 240
            LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT
Sbjct: 181  LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT 240

Query: 241  IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC 300
            IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC
Sbjct: 241  IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC 300

Query: 301  VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL 360
            VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL
Sbjct: 301  VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL 360

Query: 361  GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE 420
            GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE
Sbjct: 361  GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE 420

Query: 421  SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI 480
            SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI
Sbjct: 421  SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI 480

Query: 481  LAEDEAASVLYNIQNTLHQYCDVFLFYPPSLSCFRRAYDAERNGCDENVNMLLSIVVAAF 540
            LAEDEAASVLYNIQNTLHQYCDVFLF      C RRAYDAERNGCDENVNMLLSIVVAAF
Sbjct: 481  LAEDEAASVLYNIQNTLHQYCDVFLF------CQRRAYDAERNGCDENVNMLLSIVVAAF 540

Query: 541  TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL 600
            TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL
Sbjct: 541  TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL 600

Query: 601  CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK 660
            CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK
Sbjct: 601  CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK 660

Query: 661  TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS 720
            TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS
Sbjct: 661  TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS 720

Query: 721  KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR 780
            KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR
Sbjct: 721  KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR 780

Query: 781  ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS 840
            ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS
Sbjct: 781  ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS 840

Query: 841  KQQVLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY 900
            KQ VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY
Sbjct: 841  KQ-VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY 900

Query: 901  SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN 960
            SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN
Sbjct: 901  SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN 960

Query: 961  CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS 1020
            CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS
Sbjct: 961  CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS 1020

Query: 1021 IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV 1080
            IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV
Sbjct: 1021 IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV 1080

Query: 1081 SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG 1140
            SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG
Sbjct: 1081 SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG 1140

Query: 1141 NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI 1200
            NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI
Sbjct: 1141 NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI 1200

Query: 1201 TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS 1260
            TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS
Sbjct: 1201 TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS 1260

Query: 1261 ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS 1320
            ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS
Sbjct: 1261 ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS 1320

Query: 1321 IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC 1380
            IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC
Sbjct: 1321 IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC 1380

Query: 1381 WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI 1440
            WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI
Sbjct: 1381 WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI 1440

Query: 1441 VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC 1500
            VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC
Sbjct: 1441 VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC 1500

Query: 1501 CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD 1560
            CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD
Sbjct: 1501 CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD 1560

Query: 1561 SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE 1620
            SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE
Sbjct: 1561 SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE 1620

Query: 1621 FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV 1680
            FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV
Sbjct: 1621 FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV 1680

Query: 1681 ETILKEDYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTED 1740
            ETILKEDYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTED
Sbjct: 1681 ETILKEDYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTED 1740

Query: 1741 TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR 1800
            TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR
Sbjct: 1741 TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR 1800

Query: 1801 SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD 1860
            SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD
Sbjct: 1801 SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD 1860

Query: 1861 QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS 1920
            QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS
Sbjct: 1861 QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS 1920

Query: 1921 ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA 1980
            ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA
Sbjct: 1921 ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA 1980

Query: 1981 GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY 2040
            GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY
Sbjct: 1981 GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY 2040

Query: 2041 YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK 2100
            YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK
Sbjct: 2041 YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK 2100

Query: 2101 EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA 2160
            EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA
Sbjct: 2101 EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA 2160

Query: 2161 SPVCYGVPTSIKKKKDV 2177
            SPVCYGVPTSIKKKKDV
Sbjct: 2161 SPVCYGVPTSIKKKKDV 2170

BLAST of MC10g0420 vs. ExPASy TrEMBL
Match: A0A6J1D6B5 (Separase OS=Momordica charantia OX=3673 GN=LOC111017677 PE=4 SV=1)

HSP 1 Score: 4330 bits (11231), Expect = 0.0
Identity = 2168/2177 (99.59%), Postives = 2168/2177 (99.59%), Query Frame = 0

Query: 1    MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR 60
            MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR
Sbjct: 1    MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR 60

Query: 61   FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ 120
            FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ
Sbjct: 61   FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL 180
            RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL
Sbjct: 121  RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL 180

Query: 181  LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT 240
            LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT
Sbjct: 181  LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT 240

Query: 241  IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC 300
            IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC
Sbjct: 241  IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC 300

Query: 301  VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL 360
            VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL
Sbjct: 301  VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL 360

Query: 361  GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE 420
            GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE
Sbjct: 361  GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE 420

Query: 421  SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI 480
            SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI
Sbjct: 421  SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI 480

Query: 481  LAEDEAASVLYNIQNTLHQYCDVFLFYPPSLSCFRRAYDAERNGCDENVNMLLSIVVAAF 540
            LAEDEAASVLYNIQNTLHQYCDVFLF      C RRAYDAERNGCDENVNMLLSIVVAAF
Sbjct: 481  LAEDEAASVLYNIQNTLHQYCDVFLF------CQRRAYDAERNGCDENVNMLLSIVVAAF 540

Query: 541  TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL 600
            TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL
Sbjct: 541  TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL 600

Query: 601  CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK 660
            CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK
Sbjct: 601  CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK 660

Query: 661  TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS 720
            TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS
Sbjct: 661  TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS 720

Query: 721  KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR 780
            KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR
Sbjct: 721  KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR 780

Query: 781  ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS 840
            ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS
Sbjct: 781  ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS 840

Query: 841  KQQVLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY 900
            KQ VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY
Sbjct: 841  KQ-VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY 900

Query: 901  SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN 960
            SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN
Sbjct: 901  SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN 960

Query: 961  CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS 1020
            CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS
Sbjct: 961  CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS 1020

Query: 1021 IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV 1080
            IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV
Sbjct: 1021 IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV 1080

Query: 1081 SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG 1140
            SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG
Sbjct: 1081 SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG 1140

Query: 1141 NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI 1200
            NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI
Sbjct: 1141 NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI 1200

Query: 1201 TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS 1260
            TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS
Sbjct: 1201 TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS 1260

Query: 1261 ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS 1320
            ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS
Sbjct: 1261 ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS 1320

Query: 1321 IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC 1380
            IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC
Sbjct: 1321 IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC 1380

Query: 1381 WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI 1440
            WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI
Sbjct: 1381 WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI 1440

Query: 1441 VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC 1500
            VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC
Sbjct: 1441 VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC 1500

Query: 1501 CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD 1560
            CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD
Sbjct: 1501 CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD 1560

Query: 1561 SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE 1620
            SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE
Sbjct: 1561 SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE 1620

Query: 1621 FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV 1680
            FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV
Sbjct: 1621 FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV 1680

Query: 1681 ETILKEDYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTED 1740
            ETILK DYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTED
Sbjct: 1681 ETILK-DYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTED 1740

Query: 1741 TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR 1800
            TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR
Sbjct: 1741 TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR 1800

Query: 1801 SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD 1860
            SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD
Sbjct: 1801 SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD 1860

Query: 1861 QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS 1920
            QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS
Sbjct: 1861 QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS 1920

Query: 1921 ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA 1980
            ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA
Sbjct: 1921 ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA 1980

Query: 1981 GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY 2040
            GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY
Sbjct: 1981 GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY 2040

Query: 2041 YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK 2100
            YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK
Sbjct: 2041 YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK 2100

Query: 2101 EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA 2160
            EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA
Sbjct: 2101 EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA 2160

Query: 2161 SPVCYGVPTSIKKKKDV 2177
            SPVCYGVPTSIKKKKDV
Sbjct: 2161 SPVCYGVPTSIKKKKDV 2169

BLAST of MC10g0420 vs. ExPASy TrEMBL
Match: A0A6J1D524 (Separase OS=Momordica charantia OX=3673 GN=LOC111017677 PE=4 SV=1)

HSP 1 Score: 4200 bits (10892), Expect = 0.0
Identity = 2116/2177 (97.20%), Postives = 2116/2177 (97.20%), Query Frame = 0

Query: 1    MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR 60
            MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR
Sbjct: 1    MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR 60

Query: 61   FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ 120
            FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ
Sbjct: 61   FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL 180
            RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL
Sbjct: 121  RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL 180

Query: 181  LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT 240
            LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT
Sbjct: 181  LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT 240

Query: 241  IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC 300
            IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC
Sbjct: 241  IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLIC 300

Query: 301  VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL 360
            VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL
Sbjct: 301  VLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPL 360

Query: 361  GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE 420
            GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE
Sbjct: 361  GMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNE 420

Query: 421  SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI 480
            SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI
Sbjct: 421  SCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKI 480

Query: 481  LAEDEAASVLYNIQNTLHQYCDVFLFYPPSLSCFRRAYDAERNGCDENVNMLLSIVVAAF 540
            LAEDEAASVLYNIQNTLHQYCDVFLF      C RRAYDAERNGCDENVNMLLSIVVAAF
Sbjct: 481  LAEDEAASVLYNIQNTLHQYCDVFLF------CQRRAYDAERNGCDENVNMLLSIVVAAF 540

Query: 541  TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL 600
            TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL
Sbjct: 541  TLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKL 600

Query: 601  CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK 660
            CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK
Sbjct: 601  CCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEK 660

Query: 661  TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS 720
            TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS
Sbjct: 661  TMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMS 720

Query: 721  KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR 780
            KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR
Sbjct: 721  KAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKAR 780

Query: 781  ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS 840
            ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS
Sbjct: 781  ALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNS 840

Query: 841  KQQVLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY 900
            KQ VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY
Sbjct: 841  KQ-VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIY 900

Query: 901  SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN 960
            SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN
Sbjct: 901  SLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTN 960

Query: 961  CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS 1020
            CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS
Sbjct: 961  CLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSS 1020

Query: 1021 IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV 1080
            IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV
Sbjct: 1021 IFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHV 1080

Query: 1081 SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG 1140
            SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG
Sbjct: 1081 SQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIG 1140

Query: 1141 NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI 1200
            NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI
Sbjct: 1141 NGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSI 1200

Query: 1201 TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS 1260
            TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS
Sbjct: 1201 TSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDS 1260

Query: 1261 ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS 1320
            ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS
Sbjct: 1261 ISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRS 1320

Query: 1321 IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC 1380
            IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC
Sbjct: 1321 IQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGC 1380

Query: 1381 WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI 1440
            WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI
Sbjct: 1381 WQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSI 1440

Query: 1441 VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC 1500
            VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC
Sbjct: 1441 VVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIIC 1500

Query: 1501 CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD 1560
            CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD
Sbjct: 1501 CPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTD 1560

Query: 1561 SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE 1620
            SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE
Sbjct: 1561 SHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEE 1620

Query: 1621 FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV 1680
            FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV
Sbjct: 1621 FARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPV 1680

Query: 1681 ETILKEDYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTED 1740
            ETILKED                                                     
Sbjct: 1681 ETILKED----------------------------------------------------- 1740

Query: 1741 TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR 1800
            TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR
Sbjct: 1741 TKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLR 1800

Query: 1801 SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD 1860
            SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD
Sbjct: 1801 SKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVD 1860

Query: 1861 QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS 1920
            QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS
Sbjct: 1861 QLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGS 1920

Query: 1921 ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA 1980
            ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA
Sbjct: 1921 ICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKA 1980

Query: 1981 GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY 2040
            GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY
Sbjct: 1981 GYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGY 2040

Query: 2041 YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK 2100
            YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK
Sbjct: 2041 YVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTK 2100

Query: 2101 EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA 2160
            EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA
Sbjct: 2101 EFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGA 2117

Query: 2161 SPVCYGVPTSIKKKKDV 2177
            SPVCYGVPTSIKKKKDV
Sbjct: 2161 SPVCYGVPTSIKKKKDV 2117

BLAST of MC10g0420 vs. ExPASy TrEMBL
Match: A0A6J1D7N2 (Separase OS=Momordica charantia OX=3673 GN=LOC111017677 PE=4 SV=1)

HSP 1 Score: 4107 bits (10651), Expect = 0.0
Identity = 2046/2054 (99.61%), Postives = 2046/2054 (99.61%), Query Frame = 0

Query: 124  MVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLLLVE 183
            MVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLLLVE
Sbjct: 1    MVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLLLVE 60

Query: 184  VVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCTIFL 243
            VVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCTIFL
Sbjct: 61   VVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCTIFL 120

Query: 244  VEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLICVLK 303
            VEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLICVLK
Sbjct: 121  VEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLICVLK 180

Query: 304  SITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPLGMI 363
            SITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPLGMI
Sbjct: 181  SITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQATTPLGMI 240

Query: 364  LRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNESCS 423
            LRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNESCS
Sbjct: 241  LRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGCREGNESCS 300

Query: 424  IKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKILAE 483
            IKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKILAE
Sbjct: 301  IKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVNSERKKILAE 360

Query: 484  DEAASVLYNIQNTLHQYCDVFLFYPPSLSCFRRAYDAERNGCDENVNMLLSIVVAAFTLS 543
            DEAASVLYNIQNTLHQYCDVFLF      C RRAYDAERNGCDENVNMLLSIVVAAFTLS
Sbjct: 361  DEAASVLYNIQNTLHQYCDVFLF------CQRRAYDAERNGCDENVNMLLSIVVAAFTLS 420

Query: 544  FRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKLCCR 603
            FRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKLCCR
Sbjct: 421  FRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEASKALKLCCR 480

Query: 604  ASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEKTMA 663
            ASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEKTMA
Sbjct: 481  ASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCDIYKIEKTMA 540

Query: 664  KILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMSKAK 723
            KILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMSKAK
Sbjct: 541  KILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLPPSGMMSKAK 600

Query: 724  IGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKARALR 783
            IGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKARALR
Sbjct: 601  IGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARVLIKKARALR 660

Query: 784  FCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNSKQQ 843
            FCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNSKQ 
Sbjct: 661  FCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTHEAVPNSKQ- 720

Query: 844  VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIYSLM 903
            VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIYSLM
Sbjct: 721  VLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMDYHHNIYSLM 780

Query: 904  IRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTNCLK 963
            IRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTNCLK
Sbjct: 781  IRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKSFDFWTNCLK 840

Query: 964  TLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSSIFF 1023
            TLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSSIFF
Sbjct: 841  TLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHIPASKSSIFF 900

Query: 1024 AGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHVSQK 1083
            AGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHVSQK
Sbjct: 901  AGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKYCEIAHVSQK 960

Query: 1084 PAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIGNGS 1143
            PAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIGNGS
Sbjct: 961  PAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGLVHEYIGNGS 1020

Query: 1144 EAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSITSC 1203
            EAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSITSC
Sbjct: 1021 EAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDSITSC 1080

Query: 1204 LKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDSISC 1263
            LKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDSISC
Sbjct: 1081 LKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNLSVWKDSISC 1140

Query: 1264 PDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRSIQS 1323
            PDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRSIQS
Sbjct: 1141 PDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLTRSRCRSIQS 1200

Query: 1324 QSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGCWQC 1383
            QSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGCWQC
Sbjct: 1201 QSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCKNGKVGCWQC 1260

Query: 1384 LPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSIVVS 1443
            LPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSIVVS
Sbjct: 1261 LPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETIFKSMSIVVS 1320

Query: 1444 RNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIICCPL 1503
            RNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIICCPL
Sbjct: 1321 RNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYNDSKIICCPL 1380

Query: 1504 SQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTDSHW 1563
            SQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTDSHW
Sbjct: 1381 SQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSSNQILTDSHW 1440

Query: 1564 ASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEEFAR 1623
            ASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEEFAR
Sbjct: 1441 ASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPISSQDLEEFAR 1500

Query: 1624 KFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPVETI 1683
            KFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPVETI
Sbjct: 1501 KFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPVETI 1560

Query: 1684 LKEDYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTEDTKA 1743
            LKEDYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTEDTKA
Sbjct: 1561 LKEDYSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTEDTKA 1620

Query: 1744 NRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLRSKC 1803
            NRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLRSKC
Sbjct: 1621 NRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLRSKC 1680

Query: 1804 KMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVDQLS 1863
            KMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVDQLS
Sbjct: 1681 KMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDPFQNTFNGVDQLS 1740

Query: 1864 ALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGSICA 1923
            ALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGSICA
Sbjct: 1741 ALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGSICA 1800

Query: 1924 TLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKAGYA 1983
            TLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKAGYA
Sbjct: 1801 TLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKAGYA 1860

Query: 1984 PTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGYYVP 2043
            PTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGYYVP
Sbjct: 1861 PTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGYYVP 1920

Query: 2044 QGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTKEFE 2103
            QGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTKEFE
Sbjct: 1921 QGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPSSPRCDILTKEFE 1980

Query: 2104 TINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGASPV 2163
            TINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGASPV
Sbjct: 1981 TINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREACNLRYLIGASPV 2040

Query: 2164 CYGVPTSIKKKKDV 2177
            CYGVPTSIKKKKDV
Sbjct: 2041 CYGVPTSIKKKKDV 2047

BLAST of MC10g0420 vs. ExPASy TrEMBL
Match: A0A6J1FW16 (Separase OS=Cucurbita moschata OX=3662 GN=LOC111447831 PE=4 SV=1)

HSP 1 Score: 3523 bits (9135), Expect = 0.0
Identity = 1772/2186 (81.06%), Postives = 1925/2186 (88.06%), Query Frame = 0

Query: 1    MASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKR 60
            MASPSE+++IS+LETADSKGI S VSDFL+PFSD KNPKK K+SAKPSDDSSAIRSLAK+
Sbjct: 1    MASPSEASIISILETADSKGIFSTVSDFLRPFSDIKNPKKSKKSAKPSDDSSAIRSLAKK 60

Query: 61   FLTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQ 120
            FL FLNRALSILPKRL++P KLGNDL+LALEFF++Y+LCL CLESL SQLSCKPYTVDVQ
Sbjct: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEIYRLCLACLESLTSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLL 180
            RVRMVHCMEDWGL+KDAE EGFRILE LR I   S     DC++  D DKGGGDK+F LL
Sbjct: 121  RVRMVHCMEDWGLFKDAEAEGFRILERLRVIDRTS-----DCRVIHDRDKGGGDKEFCLL 180

Query: 181  LVEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCT 240
              EVVVT+VKC ASGRSK+SGDY R+LGLVEEV+PWFRLLD KVSEKT RAL TYL KCT
Sbjct: 181  FAEVVVTLVKCAASGRSKESGDYGRLLGLVEEVRPWFRLLDAKVSEKTQRALATYLGKCT 240

Query: 241  IFLVEELACFGGSLTSLFCRTTLAEYAKSSLRDQIYKVARRICSTLFSQHLKEDSSMLI- 300
            IFLVEEL  FG SL SLFCR T AEYAKSSL+DQIYK+ARRICSTLFS   ++ SSMLI 
Sbjct: 241  IFLVEELDYFGESLVSLFCRITFAEYAKSSLKDQIYKLARRICSTLFSLQQEQHSSMLIM 300

Query: 301  ----CVLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQ 360
                CV+KS+TLE K+E     VEFLQLI YSANKC S SP+CCCAFAKHL++MAG+ HQ
Sbjct: 301  HILICVVKSLTLEWKDETEWEVVEFLQLICYSANKCLSTSPECCCAFAKHLQEMAGEFHQ 360

Query: 361  ATTPLGMILRLYAAGLKIFSKLPRGETHDSAFSILVDDGESMQGLANLNGYLGSYFRIGC 420
            AT PLGM LRLYAAGLKI SKLPRGET  SAFS L+DD +++Q L NLNG+LGSYF IGC
Sbjct: 361  ATPPLGMNLRLYAAGLKIVSKLPRGETCGSAFSTLLDDEDTIQSLVNLNGFLGSYFCIGC 420

Query: 421  REGNESCSIKQKDFV-QPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLAELVN 480
            REGNESCSI+QKDFV QPC H NSNHE  V SL   RE YL SYLDAIKF CQPLAE VN
Sbjct: 421  REGNESCSIEQKDFVGQPCLHRNSNHENGVPSLLSLREAYLSSYLDAIKFFCQPLAESVN 480

Query: 481  SERKKILAEDEAASVLYNIQNTLHQYCDVFLFYPPSLSCFRRAYDAERNGCDENVNMLLS 540
             ERK+ILAED+AA VLYNIQNTLHQ+CDVFLF      C RRAYDA+ +GCDENVNMLLS
Sbjct: 481  VERKEILAEDKAAPVLYNIQNTLHQFCDVFLF------CQRRAYDAKSDGCDENVNMLLS 540

Query: 541  IVVAAFTLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYRHKQIKEA 600
            IVVAAFTLSFRT+L+MKRST LIK++I SKWVQP ALK LFASLNNIGIIL+R+KQI EA
Sbjct: 541  IVVAAFTLSFRTRLDMKRSTNLIKDVIASKWVQPFALKRLFASLNNIGIILFRNKQIGEA 600

Query: 601  SKALKLCCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLVDILYQCD 660
            SKALKLCCRASWTCV  FCE FD+KSRPSDNEFSE++VL  VNE+IMRSAFLVDILYQCD
Sbjct: 601  SKALKLCCRASWTCVKQFCEIFDNKSRPSDNEFSENSVLIIVNEVIMRSAFLVDILYQCD 660

Query: 661  IYKIEKTMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTPTLNCLLP 720
            + K+EK M +IL NWS AAKLF+ELP+PVQLVKQWVKMQCK+HKNADPK +TPTLNCLL 
Sbjct: 661  MCKVEKAMTEILTNWSAAAKLFDELPVPVQLVKQWVKMQCKYHKNADPKKSTPTLNCLLQ 720

Query: 721  PSGMMSKAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDHMLERARV 780
             S  +SKA+IG LLEQELVEYEEMS LYP FCQ LQMKILS LL D YITPDH LE+ARV
Sbjct: 721  SSETVSKARIGVLLEQELVEYEEMSGLYPEFCQSLQMKILSILLTDTYITPDHRLEKARV 780

Query: 781  LIKKARALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYCLRALCTH 840
            L+KKAR LR CETVCL+  IQCLS+AISTMNE SSG+ S GVLH HQLAVAYCLRA CTH
Sbjct: 781  LMKKARVLRLCETVCLDGCIQCLSEAISTMNEISSGSRSFGVLHSHQLAVAYCLRAFCTH 840

Query: 841  EAVPNSKQQVLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLLSIKGCMD 900
            E VPNSKQ V QDIDSALS+WLEI SL++LPDDQR ILSEYMLLLLCN FDLLSIKGC+D
Sbjct: 841  ETVPNSKQ-VQQDIDSALSIWLEISSLNTLPDDQRLILSEYMLLLLCNAFDLLSIKGCVD 900

Query: 901  YHHNIYSLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEHLGELPKS 960
            YH NIYSLMIRLFKWKNV LEKL+A LWESRRM HAMC AP NE+ IAQLSEHLG+LPKS
Sbjct: 901  YHDNIYSLMIRLFKWKNVPLEKLMATLWESRRMSHAMCIAPANEIVIAQLSEHLGDLPKS 960

Query: 961  FDFWTNCLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAASKLISHI 1020
             DFWTNCLKTLP MLVGFQQNFSFLCSNYTQ+SCEHEKS +++VTVDE KEAA KLISHI
Sbjct: 961  NDFWTNCLKTLPGMLVGFQQNFSFLCSNYTQTSCEHEKSSRMHVTVDEVKEAALKLISHI 1020

Query: 1021 PASKSSIFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSVEQHPEKY 1080
            PA KSSIF AGYLYYDLCERLISEGCLTEAL CAKEAH+LRSKLFQE F YSVEQHPEKY
Sbjct: 1021 PACKSSIFSAGYLYYDLCERLISEGCLTEALLCAKEAHRLRSKLFQEKFTYSVEQHPEKY 1080

Query: 1081 CEIAHVSQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLESTLQVGL 1140
            CEI H SQKP YGIKNLRK+GSV+RN+WSFD+ISWDVE CYLSPFNVLQCYLESTLQVGL
Sbjct: 1081 CEITHASQKPPYGIKNLRKSGSVSRNIWSFDKISWDVEGCYLSPFNVLQCYLESTLQVGL 1140

Query: 1141 VHEYIGNGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQ 1200
            VHE IGNGSEAETLLQWGKSISCL+SLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQ
Sbjct: 1141 VHEIIGNGSEAETLLQWGKSISCLRSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQ 1200

Query: 1201 ILTDSITSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLYKLALEKLNL 1260
            ILTD+ITSCLKCRLVLEVIVDQ+LGDLFRSMYVN NGN SEE LINAE+LYKLALEKLNL
Sbjct: 1201 ILTDNITSCLKCRLVLEVIVDQYLGDLFRSMYVNGNGNTSEELLINAEELYKLALEKLNL 1260

Query: 1261 SVWKDSISCPDEDRYLSSLTTQAERPKAKRDGKKCKKAINTSRSFQMDQCINPQSNVRLT 1320
            S WK+SISCPDED +LSSLT   ER KA++DG+K KK  N  RS +MDQC+NPQ+NVRLT
Sbjct: 1261 SGWKNSISCPDEDSHLSSLTIPVERAKARKDGRKSKKTTNAPRSLKMDQCVNPQTNVRLT 1320

Query: 1321 RSRCRSIQSQSTSNSNDVEVDLSVHLKSNVPDRSSASGQRQLHLQINSCTPTSVCGASCK 1380
            RSRCRSIQ QST+ SN+VEVDL+VHLKSNVPD   ASGQ+Q+HLQ+N CTPT  CGASCK
Sbjct: 1321 RSRCRSIQGQSTNISNEVEVDLTVHLKSNVPDLFGASGQKQMHLQVNCCTPTLGCGASCK 1380

Query: 1381 NGKVGCWQCLPMEITEAGQMNNFIYLKWEFVRKRLLLRQLSGLGKCLGIRGQFHQTHETI 1440
            NGKVGCWQCL MEI EAGQ+NNFIYLKWEFVR+RLLLRQLSGLGKC GIRGQ HQTHETI
Sbjct: 1381 NGKVGCWQCLQMEIMEAGQINNFIYLKWEFVRRRLLLRQLSGLGKCSGIRGQIHQTHETI 1440

Query: 1441 FKSMSIVVSRNLFFQAHYAVEPTVLLDLVGKEVHGDMFAVERASVLYNVCWFSLKSCKYN 1500
             KSMSI+VSRN+F QAH  VEP VLLDLVGKEV GDMFAVERASVLYN+CWF+LKS KY+
Sbjct: 1441 LKSMSILVSRNIFSQAHGVVEPVVLLDLVGKEVPGDMFAVERASVLYNICWFALKSYKYD 1500

Query: 1501 DSKIICCPLSQVQFKTLASWLMVALVLCSEVPVLLQKVSKLLAAVHVISSSRELFSLPSS 1560
            D+KIICCPLSQVQ KTL SWLMVALVLC EVPVL QKVSKLLA +HVISSS EL SLPSS
Sbjct: 1501 DTKIICCPLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSIELLSLPSS 1560

Query: 1561 NQILTDSHWASYFHQASIGTHLNYQYFPYRTGSSCVQDLNFAKGFDTGEQTLKSRRGPIS 1620
            N+ILTDSHWASYFHQASIGTHLN+Q+FPY TG SC+QDLNFAKGFDTGEQT+K  RG  S
Sbjct: 1561 NKILTDSHWASYFHQASIGTHLNHQFFPYTTGRSCIQDLNFAKGFDTGEQTVKLFRGSFS 1620

Query: 1621 SQDLEEFARKFFDGLPCMTIVCISLIGGDLACLLQQTLDYPSSVHGWILVSYLNSKYQPL 1680
            SQDLEE+ RKFFDGLPC+ +VCISLIGGDLACLLQQ L +PSSVH WILVS+LNSK QPL
Sbjct: 1621 SQDLEEYVRKFFDGLPCVAMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLNSKRQPL 1680

Query: 1681 VVLLPVETILKED---YSNPQSDDIYERNDLNKHWKCPWGSSVIDEVAPAFRIILQENYL 1740
            V+LLPVETILKED   YSNPQSDDI ERND  KHW+CPWGSSVIDE+APAFR IL++NYL
Sbjct: 1681 VLLLPVETILKEDSEDYSNPQSDDICERNDSTKHWQCPWGSSVIDEIAPAFRTILEDNYL 1740

Query: 1741 SSSVFPTEDTKANRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKHMLLGELTNRKHADSV 1800
            SSSVFP+EDTK NRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWK++LLG+ +NRKH DSV
Sbjct: 1741 SSSVFPSEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYILLGDWSNRKHVDSV 1800

Query: 1801 LNTLVLDLRSKCKMDVNESLLKVILEGPEEVLEAFGSNLNSRKGCFVGKVGLHDKQRSDP 1860
            LNTLVL+L+SKCKMDVNE LLK+ILEG E VL  F S L SRKGCFVG+   HDK+RS+P
Sbjct: 1801 LNTLVLNLKSKCKMDVNEGLLKIILEGSENVLVGFDSKLYSRKGCFVGRARFHDKERSNP 1860

Query: 1861 FQNTFNGVDQLSALALKLIQDAKKELEGEDNSSREPIIFVLDYDVQMLPWENIPILQNQE 1920
            FQN  NGVD+LS LALKLIQDAKKELEGED +SREPII VLDYDVQMLPWENIPIL+NQE
Sbjct: 1861 FQNALNGVDKLSTLALKLIQDAKKELEGEDGTSREPIILVLDYDVQMLPWENIPILKNQE 1920

Query: 1921 VYRMPSVGSICATLDRRCRQQEQVSGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWF 1980
            VYRMPSVGSIC TLD R  QQEQ SGII  FPSIDP D+FYLLNPSGDL +TQIEFENWF
Sbjct: 1921 VYRMPSVGSICVTLDGRLHQQEQDSGIIATFPSIDPLDAFYLLNPSGDLSSTQIEFENWF 1980

Query: 1981 KDQNLEGKAGYAPTSAELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCS 2040
            KDQNLEGKAGYAP S+ELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLD CAASLLMGCS
Sbjct: 1981 KDQNLEGKAGYAPKSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAASLLMGCS 2040

Query: 2041 SGSLTLNGYYVPQGVPLSYLKAGSPVIVANLWEVTDKDIDRFGKAVLEAWLRERSSVLPS 2100
            SGSLTLNGYYVPQG+PLSYLKAGSPVIVANLWEVTDKDIDRFGKA+LEAWLRERS  LPS
Sbjct: 2041 SGSLTLNGYYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRERSCALPS 2100

Query: 2101 SPRCDILTKEFETINISSKRISKKVASKNPPAACESDSSSRGRPVTSRMIGSFLYEAREA 2160
            S +CDI+TKE E + ISSK  +KKVASK+ P  CES SSS+   V SRMIGSFL EAREA
Sbjct: 2101 SVQCDIVTKELEAMKISSKLANKKVASKSLPTTCES-SSSKDHSVHSRMIGSFLCEAREA 2160

Query: 2161 CNLRYLIGASPVCYGVPTSIKKKKDV 2177
            CNLRYLIGASPVCYGVPTSI+KKK++
Sbjct: 2161 CNLRYLIGASPVCYGVPTSIRKKKEL 2173

BLAST of MC10g0420 vs. TAIR 10
Match: AT4G22970.2 (homolog of separase )

HSP 1 Score: 1687.9 bits (4370), Expect = 0.0e+00
Identity = 970/2223 (43.63%), Postives = 1340/2223 (60.28%), Query Frame = 0

Query: 2    ASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKRF 61
            +S  +  L+S+++  D+  + S  SD+L+PFS          S K  D ++ IR+LAK+F
Sbjct: 3    SSGDDLRLLSLIDVGDN--VFSSFSDYLKPFSTLST------SRKKQDRATTIRALAKQF 62

Query: 62   LTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQR 121
            L FLN+++S+LPKRL+         + AL+ F+ Y+LCL CLE + +QL+CKP+TV  QR
Sbjct: 63   LPFLNKSISLLPKRLSVANSDKEARESALDLFRAYELCLDCLELVSAQLACKPHTVQSQR 122

Query: 122  VRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLLL 181
            +RM+HC++ WGLY++   E F++LE LR    +S+  +L     P+V    GD +  L++
Sbjct: 123  LRMIHCLDVWGLYENVYTEAFKVLEKLRGSDSKSRKSRL----LPEVQ--DGDAEMALVV 182

Query: 182  VEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCTI 241
            V+ V  I +  A  +  D   Y +VL L+EEV  W R+LD KV EK HRA+VT + KC +
Sbjct: 183  VDAVAAIFRAVAMSQQLDDKRYRKVLLLLEEVGGWLRVLDAKVYEKLHRAMVTSMGKCAV 242

Query: 242  FLVEELACFGGSLTSLFCRTTLAEYAKSSL-RDQIYKVARRICSTLF---SQHLKEDSSM 301
             LV E   F G L   FC  T+ E+ KS+L +D++YK AR + S LF    + +     +
Sbjct: 243  SLVREAERFNGDLVISFCDLTVKEHYKSALSKDRVYKFAREVLSVLFGFKDRKMSVTIDI 302

Query: 302  LICVLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQAT 361
             + VL+S++ + ++E     +EF  L+ Y A+K ++A    C   +K L +MA    +A 
Sbjct: 303  SMSVLRSLSCQFEDESNENLMEFFDLVDYCAHKFRAAGDMYCAKVSKKLNEMAAIFVEAI 362

Query: 362  TPLGMILRLYAAGLKIF---SKLPRGETHDSA----FSILVDDGESMQGLANLNGYLGSY 421
              L ++LRLY+ GL I    SKL   +  DS        + DD    Q L +L G + SY
Sbjct: 363  PQLNLVLRLYSTGLSITVCNSKLGEIKLEDSTDDWKIQAMFDDDARWQSLVSLLGMVDSY 422

Query: 422  FRIGCREGNESCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLA 481
                  EGN++ S           H N N++   +   R + T  P Y+DA+KFLCQPLA
Sbjct: 423  ---SGDEGNQTGSSSIG------GHRNYNNKTHDSCKDRNKITCWPQYVDALKFLCQPLA 482

Query: 482  ELVNSERKKILAEDE---AASVLYNIQNTLHQYCDVFLFYPPSLSCFRRAYDAERNGCDE 541
            + + S ++KI+ E E   A++ L  I +   Q+CD  LF        +R    + +    
Sbjct: 483  DFIYSVKRKIVLETEMSCASAHLITIHDAFLQFCDGCLF-------LQRCTSDKGDREIA 542

Query: 542  NVNMLLSIVVAAFTLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYR 601
            N    L+  + AF +S RT+L ++ S +L++++I S W+Q   LK+L A+L NIGI+LYR
Sbjct: 543  NNKAFLNAAMGAFIVSLRTQLKLEISAHLVEDVIGSPWIQSQELKYLIATLYNIGIVLYR 602

Query: 602  HKQIKEASKALKLCCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLV 661
            +K++ +A +ALKLC + SW CV   C  F ++S  SDN+ SEDA++ FV E   R AF +
Sbjct: 603  NKELNKACEALKLCSKVSWRCVELHCHMFVNQSSSSDNDLSEDAIMDFVGEACNRCAFYL 662

Query: 662  DILYQCDIYKIEKTMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTP 721
            DIL +C   KI + +  ILENW  A  L   LP P  +VKQWVK++ + H + D   +  
Sbjct: 663  DILQKCSRRKIRQNIVHILENWLSAEHLIRRLPGPEAIVKQWVKIERECHTDLDAAGSCT 722

Query: 722  TLNCLLPPSGMMSKAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDH 781
            TL  LL  S   SK  IG +LEQEL+ Y+ +  L     Q  ++KI   LL+DVY+T D 
Sbjct: 723  TLYSLLSSSQKKSKRGIGKILEQELLAYDRVLPLRSNLGQQTRIKIADILLKDVYVTEDM 782

Query: 782  MLERARVLIKKARALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYC 841
             +ERAR+LI KAR  R   T  + + I  LS+AIS + E   G    G    H L +AYC
Sbjct: 783  HIERARILIWKARMTRTSGTEHITECICFLSEAISILGELHHGPNEEGSPSSHMLPIAYC 842

Query: 842  LRALCTHEAVPNSKQQVLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLL 901
            LRA CT EA PNSK +V QDI ++L++WL ILSLD   D    + +E ++ LL N  DL+
Sbjct: 843  LRAFCTQEADPNSK-KVFQDISTSLNLWLRILSLDDSGDS---LPTENIIPLLYNMIDLM 902

Query: 902  SIKGCMDYHHNIYSLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEH 961
            S+KGC + HH+IY L+ RLFKWKNV+LE  LA+LWE RR+ HA+C +P+++ FI  LSE+
Sbjct: 903  SVKGCTELHHHIYQLIFRLFKWKNVKLEVCLAMLWECRRLSHALCPSPISDAFIQTLSEN 962

Query: 962  LGELPKSFDFWTNCLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAA 1021
              +     DFW +CLK     L+GFQQNF  L +       + E   Q  +T+D+ K+AA
Sbjct: 963  CADKSTCIDFWMDCLKDSKAKLIGFQQNFHDLHN-------KDEGPFQSDITIDDIKDAA 1022

Query: 1022 SKLISHIPASKSSIFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSV 1081
            S+LIS    S +S F A YLYYDLCERLIS G L+EALS AKEA+++R+ +FQ+ F Y+ 
Sbjct: 1023 SELISSASLSGNSSFAAAYLYYDLCERLISFGKLSEALSYAKEAYRIRTLIFQDKFKYTA 1082

Query: 1082 EQHPEKYCEIAHVSQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLE 1141
            E+H EK+ E   +S+   + IKN +    +A + W      WD+  CYLSP++VLQCYLE
Sbjct: 1083 EKHIEKHNEDGKISEIRTFSIKNFQVYRLLATDFWPCGNFLWDINRCYLSPWSVLQCYLE 1142

Query: 1142 STLQVGLVHEYIGNGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQK 1201
            STLQVG+++E IGNG EAET+L WGK+ SC QSL  F VAFSSALG +Y KKQ   LA+K
Sbjct: 1143 STLQVGILNELIGNGLEAETILSWGKAFSCSQSLFPFVVAFSSALGNLYHKKQCLDLAEK 1202

Query: 1202 ELEGAKQIL--TDSITSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLY 1261
            EL+ AK+IL       SC+KC+L LEV +D+ LGD+ R      +     +  ++AE L+
Sbjct: 1203 ELQNAKEILIANQRDFSCVKCKLKLEVTLDKQLGDISRKQIDRVS---QTDGFLHAESLF 1262

Query: 1262 KLALEKLNLSVWKDSISCPDED----------------RYLSSLTTQAERPKAKRDGKKC 1321
              AL K   S WK  I    E+                   S      + P   R  ++ 
Sbjct: 1263 SAALGKFCCSAWKSCIRSHGEEIAEEIVIDRNGGEGLGHNSSKTKLSIKEPPGNRGSRRG 1322

Query: 1322 KKAINTSRSFQMDQCINPQSNVRLTRSRCRSIQSQSTSNSNDVEVDLSVHLKSNVPDRSS 1381
             +A  T  S   D    P S  RLTRS   S++ Q  + SN  EV   V  K N+ DRS 
Sbjct: 1323 GRANKTCLSKDQDLISEPTS--RLTRSMRHSLREQCQNRSNVPEV---VSKKPNLCDRSV 1382

Query: 1382 ASGQRQLHLQINSCTPTSVCGASCKNGKVGCWQCLPMEITEAGQMNNFIYLKWEFVRKRL 1441
             S   ++ L  ++  P       C   K    QCL  E+TE+G +NN + LKWE   ++L
Sbjct: 1383 GSRGERVLLDTSNALP-----GFCICYKEKRQQCLSEEVTESGSLNNLVSLKWELCHRKL 1442

Query: 1442 LLRQLSGLGKCLGIRGQFHQTHETIFKSMSIVVSRNLFFQAHYAVEPTVLLDLVGKEVHG 1501
                L  LGKCLG  G+ H  HE +  S+S++      + +H     + LL+ +GKEV  
Sbjct: 1443 ASSILVSLGKCLGDSGRIHLAHEALLHSISVLFKST--WSSHNQPSVSQLLEFIGKEVTR 1502

Query: 1502 DMFAVERASVLYNVCWFSLKSCKYNDSKIICCPLSQVQFKTLASWLMVALVLCSEVPVLL 1561
            D+FAV+RA +LYN+CW +L++     S+ ICC L  + F  L SWLM+A VL  EVP+L 
Sbjct: 1503 DVFAVDRAIILYNLCWLNLRNYHCRKSRSICCDLFHIPFTKLVSWLMLAFVLSGEVPILF 1562

Query: 1562 QKVSKLLAAVHVISSSRELFSLPSSNQILTDSHWASYFHQASIGTHLNYQY---FPYRTG 1621
            QKVS+LLA+++++SSS   F+  S    L+ SHW S+FHQAS+GTHL+Y +      +  
Sbjct: 1563 QKVSRLLASLYLLSSSNSEFTFESDGNELSASHWVSFFHQASLGTHLSYHFISNLSQKHK 1622

Query: 1622 SSCVQDLNFAKGFDTG----EQTLKSRRGPISSQDLEEFARKFFDGLPCMTIVCISLIGG 1681
            S C+ D    +   +     E     R  P  +QDL +FA++FF  LP  TI+CISL+GG
Sbjct: 1623 SQCLSDKECTEATCSSCMVPEDLDLPRLAPDRTQDLVQFAKEFFINLPSSTIICISLLGG 1682

Query: 1682 DLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPVETILKEDYSNPQSDDIYERNDL 1741
             L  LLQ+ +   S V  W+L+S LN + QP+  LLPV++I+++D +N  S +  +   L
Sbjct: 1683 ALNQLLQELMHIRSPVCAWVLISRLNPESQPVATLLPVDSIVEDDSANLSSTEATQVKSL 1742

Query: 1742 NKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTEDTKANRMLWWKRRTKLDDCLGKL 1801
               W CPWG++V+DEVAPAF+ IL+E++ SSS    EDT  +R LWWK+R KL+  LG  
Sbjct: 1743 KGPWLCPWGTTVVDEVAPAFKSILEESHSSSST-TEEDTIESRGLWWKKRKKLNHRLGIF 1802

Query: 1802 LGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLRSKCKMDVNESLLKVILEGPEEV 1861
            L  +E SWLGPW+ +LLGE +N K  DS    LV DL+SKCKM+VNE LLKVIL G  + 
Sbjct: 1803 LRNLEASWLGPWRCLLLGEWSNYKLPDSAQKKLVNDLKSKCKMEVNEMLLKVILGGGTDN 1862

Query: 1862 L--EAFGSNLNSRKGCFVGKVG-LHDKQRSDPFQNTFNGVDQLSALALKLIQDAKKELEG 1921
               EA  + L+ R GC+VG+ G L+++          N  +    LALKLI DA  +L  
Sbjct: 1863 FKGEACVAQLSLRNGCYVGRGGYLYEEDSCKTPTAASNISESRHELALKLIHDAASKLGQ 1922

Query: 1922 ED-NSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGSICATLDRRCRQQEQVSGI 1981
            +D + +REPII VLD +VQMLPWENIPIL+ QEVYRMPSVG I A L +R  Q E     
Sbjct: 1923 QDGHENREPIILVLDPEVQMLPWENIPILRKQEVYRMPSVGCISAVLKKRSLQGEPAKSH 1982

Query: 1982 IPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKAGYAPTSAELIEELKSRDL 2041
            + +FP IDP DSFYLLNP GDL +TQ+ FE+WF+DQN EGKAG  P++ EL E L++ DL
Sbjct: 1983 VASFPLIDPLDSFYLLNPGGDLTDTQVTFESWFRDQNFEGKAGSEPSAIELTEALETHDL 2042

Query: 2042 FIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGYYVPQGVPLSYLKAGSPVI 2101
            F+YFGHGSGAQYIPR EI+KLD C+A+ LMGCSSGSL L G Y+PQGVPLSYL  GSP I
Sbjct: 2043 FLYFGHGSGAQYIPRREIEKLDNCSATFLMGCSSGSLWLKGCYIPQGVPLSYLLGGSPAI 2102

Query: 2102 VANLWEVTDKDIDRFGKAVLEAWLRER--SSVLPSSPRCDILTKEFETINISSKRISKKV 2161
            VA LW+VTD+DIDRFGKA+LEAWL+ER  SS      +C+ L  +   + +   + S+K 
Sbjct: 2103 VATLWDVTDRDIDRFGKALLEAWLQERSDSSSEGGCSQCESLANDLAAMTLKGTKRSRKP 2162

Query: 2162 ASKNPPAACESDSSSR--GRPVTSRMIGSFLYEAREACNLRYLIGASPVCYGVPTSIKKK 2178
            +S+N PA  + D S +        R IGSF+  AR+ACNL+YLIGA+PVCYGVPT I +K
Sbjct: 2163 SSRNKPAQSDVDGSGKIECNHKHRRKIGSFIAAARDACNLQYLIGAAPVCYGVPTGITRK 2168

BLAST of MC10g0420 vs. TAIR 10
Match: AT4G22970.1 (homolog of separase )

HSP 1 Score: 1682.5 bits (4356), Expect = 0.0e+00
Identity = 970/2226 (43.58%), Postives = 1339/2226 (60.15%), Query Frame = 0

Query: 2    ASPSESTLISMLETADSKGISSLVSDFLQPFSDAKNPKKGKRSAKPSDDSSAIRSLAKRF 61
            +S  +  L+S+++  D+  + S  SD+L+PFS          S K  D ++ IR+LAK+F
Sbjct: 3    SSGDDLRLLSLIDVGDN--VFSSFSDYLKPFSTLST------SRKKQDRATTIRALAKQF 62

Query: 62   LTFLNRALSILPKRLTQPPKLGNDLDLALEFFQMYKLCLGCLESLMSQLSCKPYTVDVQR 121
            L FLN+++S+LPKRL+         + AL+ F+ Y+LCL CLE + +QL+CKP+TV  QR
Sbjct: 63   LPFLNKSISLLPKRLSVANSDKEARESALDLFRAYELCLDCLELVSAQLACKPHTVQSQR 122

Query: 122  VRMVHCMEDWGLYKDAEVEGFRILESLREIVLRSKAGKLDCQIFPDVDKGGGDKDFGLLL 181
            +RM+HC++ WGLY++   E F++LE LR    +S+  +L     P+V    GD +  L++
Sbjct: 123  LRMIHCLDVWGLYENVYTEAFKVLEKLRGSDSKSRKSRL----LPEVQ--DGDAEMALVV 182

Query: 182  VEVVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCTI 241
            V+ V  I +  A  +  D   Y +VL L+EEV  W R+LD KV EK HRA+VT + KC +
Sbjct: 183  VDAVAAIFRAVAMSQQLDDKRYRKVLLLLEEVGGWLRVLDAKVYEKLHRAMVTSMGKCAV 242

Query: 242  FLVEELACFGGSLTSLFCRTTLAEYAKSSL-RDQIYKVARRICSTLF---SQHLKEDSSM 301
             LV E   F G L   FC  T+ E+ KS+L +D++YK AR + S LF    + +     +
Sbjct: 243  SLVREAERFNGDLVISFCDLTVKEHYKSALSKDRVYKFAREVLSVLFGFKDRKMSVTIDI 302

Query: 302  LICVLKSITLELKEEIGCTGVEFLQLIFYSANKCQSASPDCCCAFAKHLEDMAGDIHQAT 361
             + VL+S++ + ++E     +EF  L+ Y A+K ++A    C   +K L +MA    +A 
Sbjct: 303  SMSVLRSLSCQFEDESNENLMEFFDLVDYCAHKFRAAGDMYCAKVSKKLNEMAAIFVEAI 362

Query: 362  TPLGMILRLYAAGLKIF---SKLPRGETHDSA----FSILVDDGESMQGLANLNGYLGSY 421
              L ++LRLY+ GL I    SKL   +  DS        + DD    Q L +L G + SY
Sbjct: 363  PQLNLVLRLYSTGLSITVCNSKLGEIKLEDSTDDWKIQAMFDDDARWQSLVSLLGMVDSY 422

Query: 422  FRIGCREGNESCSIKQKDFVQPCSHWNSNHEFEVTSLSRRRETYLPSYLDAIKFLCQPLA 481
                  EGN++ S           H N N++   +   R + T  P Y+DA+KFLCQPLA
Sbjct: 423  ---SGDEGNQTGSSSIG------GHRNYNNKTHDSCKDRNKITCWPQYVDALKFLCQPLA 482

Query: 482  ELVNSERKKILAEDE---AASVLYNIQNTLHQYCDVFLFYPPSLSCFRRAYDAERNGCDE 541
            + + S ++KI+ E E   A++ L  I +   Q+CD  LF        +R    + +    
Sbjct: 483  DFIYSVKRKIVLETEMSCASAHLITIHDAFLQFCDGCLF-------LQRCTSDKGDREIA 542

Query: 542  NVNMLLSIVVAAFTLSFRTKLNMKRSTYLIKEIITSKWVQPLALKHLFASLNNIGIILYR 601
            N    L+  + AF +S RT+L ++ S +L++++I S W+Q   LK+L A+L NIGI+LYR
Sbjct: 543  NNKAFLNAAMGAFIVSLRTQLKLEISAHLVEDVIGSPWIQSQELKYLIATLYNIGIVLYR 602

Query: 602  HKQIKEASKALKLCCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNEMIMRSAFLV 661
            +K++ +A +ALKLC + SW CV   C  F ++S  SDN+ SEDA++ FV E   R AF +
Sbjct: 603  NKELNKACEALKLCSKVSWRCVELHCHMFVNQSSSSDNDLSEDAIMDFVGEACNRCAFYL 662

Query: 662  DILYQCDIYKIEKTMAKILENWSIAAKLFNELPIPVQLVKQWVKMQCKHHKNADPKTTTP 721
            DIL +C   KI + +  ILENW  A  L   LP P  +VKQWVK++ + H + D   +  
Sbjct: 663  DILQKCSRRKIRQNIVHILENWLSAEHLIRRLPGPEAIVKQWVKIERECHTDLDAAGSCT 722

Query: 722  TLNCLLPPSGMMSKAKIGALLEQELVEYEEMSVLYPAFCQPLQMKILSTLLRDVYITPDH 781
            TL  LL  S   SK  IG +LEQEL+ Y+ +  L     Q  ++KI   LL+DVY+T D 
Sbjct: 723  TLYSLLSSSQKKSKRGIGKILEQELLAYDRVLPLRSNLGQQTRIKIADILLKDVYVTEDM 782

Query: 782  MLERARVLIKKARALRFCETVCLEDSIQCLSDAISTMNETSSGTCSSGVLHCHQLAVAYC 841
             +ERAR+LI KAR  R   T  + + I  LS+AIS + E   G    G    H L +AYC
Sbjct: 783  HIERARILIWKARMTRTSGTEHITECICFLSEAISILGELHHGPNEEGSPSSHMLPIAYC 842

Query: 842  LRALCTHEAVPNSKQQVLQDIDSALSMWLEILSLDSLPDDQRPILSEYMLLLLCNTFDLL 901
            LRA CT EA PNSK +V QDI ++L++WL ILSLD   D    + +E ++ LL N  DL+
Sbjct: 843  LRAFCTQEADPNSK-KVFQDISTSLNLWLRILSLDDSGDS---LPTENIIPLLYNMIDLM 902

Query: 902  SIKGCMDYHHNIYSLMIRLFKWKNVQLEKLLAILWESRRMGHAMCTAPVNEMFIAQLSEH 961
            S+KGC + HH+IY L+ RLFKWKNV+LE  LA+LWE RR+ HA+C +P+++ FI  LSE+
Sbjct: 903  SVKGCTELHHHIYQLIFRLFKWKNVKLEVCLAMLWECRRLSHALCPSPISDAFIQTLSEN 962

Query: 962  LGELPKSFDFWTNCLKTLPVMLVGFQQNFSFLCSNYTQSSCEHEKSIQLYVTVDEAKEAA 1021
              +     DFW +CLK     L+GFQQNF  L +       + E   Q  +T+D+ K+AA
Sbjct: 963  CADKSTCIDFWMDCLKDSKAKLIGFQQNFHDLHN-------KDEGPFQSDITIDDIKDAA 1022

Query: 1022 SKLISHIPASKSSIFFAGYLYYDLCERLISEGCLTEALSCAKEAHQLRSKLFQEMFMYSV 1081
            S+LIS    S +S F A YLYYDLCERLIS G L+EALS AKEA+++R+ +FQ+ F Y+ 
Sbjct: 1023 SELISSASLSGNSSFAAAYLYYDLCERLISFGKLSEALSYAKEAYRIRTLIFQDKFKYTA 1082

Query: 1082 EQHPEKYCEIAHVSQKPAYGIKNLRKNGSVARNVWSFDRISWDVESCYLSPFNVLQCYLE 1141
            E+H EK+ E   +S+   + IKN +    +A + W      WD+  CYLSP++VLQCYLE
Sbjct: 1083 EKHIEKHNEDGKISEIRTFSIKNFQVYRLLATDFWPCGNFLWDINRCYLSPWSVLQCYLE 1142

Query: 1142 STLQVGLVHEYIGNGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQK 1201
            STLQVG+++E IGNG EAET+L WGK+ SC QSL  F VAFSSALG +Y KKQ   LA+K
Sbjct: 1143 STLQVGILNELIGNGLEAETILSWGKAFSCSQSLFPFVVAFSSALGNLYHKKQCLDLAEK 1202

Query: 1202 ELEGAKQIL--TDSITSCLKCRLVLEVIVDQHLGDLFRSMYVNANGNISEERLINAEKLY 1261
            EL+ AK+IL       SC+KC+L LEV +D+ LGD+ R      +     +  ++AE L+
Sbjct: 1203 ELQNAKEILIANQRDFSCVKCKLKLEVTLDKQLGDISRKQIDRVS---QTDGFLHAESLF 1262

Query: 1262 KLALEKLNLSVWKDSISCPDED----------------RYLSSLTTQAERPKAKRDGKKC 1321
              AL K   S WK  I    E+                   S      + P   R  ++ 
Sbjct: 1263 SAALGKFCCSAWKSCIRSHGEEIAEEIVIDRNGGEGLGHNSSKTKLSIKEPPGNRGSRRG 1322

Query: 1322 KKAINTSRSFQMDQCINPQSNVRLTRSRCRSIQSQSTSNSNDVEVDLSVHLKSNVPDRSS 1381
             +A  T  S   D    P S  RLTRS   S++ Q  + SN  EV   V  K N+ DRS 
Sbjct: 1323 GRANKTCLSKDQDLISEPTS--RLTRSMRHSLREQCQNRSNVPEV---VSKKPNLCDRSV 1382

Query: 1382 ASGQRQLHLQINSCTPTSVCGASCKNGKVGCWQCLPMEITEAGQMNNFIYLKWEFVRKRL 1441
             S   ++ L  ++  P       C   K    QCL  E+TE+G +NN + LKWE   ++L
Sbjct: 1383 GSRGERVLLDTSNALP-----GFCICYKEKRQQCLSEEVTESGSLNNLVSLKWELCHRKL 1442

Query: 1442 LLRQLSGLGKCLGIRGQFHQTHETIFKSMSIVVSRNLFFQAHYAVEPTVLLDLVGKEVHG 1501
                L  LGKCLG  G+ H  HE +  S+S++      + +H     + LL+ +GKEV  
Sbjct: 1443 ASSILVSLGKCLGDSGRIHLAHEALLHSISVLFKST--WSSHNQPSVSQLLEFIGKEVTR 1502

Query: 1502 DMFAVERASVLYNVCWFSLKSCKYNDSKIICCPLSQVQFKTLASWLMVALVLCSEVPVLL 1561
            D+FAV+RA +LYN+CW +L++     S+ ICC L  + F  L SWLM+A VL  EVP+L 
Sbjct: 1503 DVFAVDRAIILYNLCWLNLRNYHCRKSRSICCDLFHIPFTKLVSWLMLAFVLSGEVPILF 1562

Query: 1562 QKVSKLLAAVHVISSSRELFSLPSSNQILTDSHWASYFHQASIGTHLNYQY---FPYRTG 1621
            QKVS+LLA+++++SSS   F+  S    L+ SHW S+FHQAS+GTHL+Y +      +  
Sbjct: 1563 QKVSRLLASLYLLSSSNSEFTFESDGNELSASHWVSFFHQASLGTHLSYHFISNLSQKHK 1622

Query: 1622 SSCVQDLNFAKGFDTG----EQTLKSRRGPISSQDLEEFARKFFDGLPCMTIVCISLIGG 1681
            S C+ D    +   +     E     R  P  +QDL +FA++FF  LP  TI+CISL+GG
Sbjct: 1623 SQCLSDKECTEATCSSCMVPEDLDLPRLAPDRTQDLVQFAKEFFINLPSSTIICISLLGG 1682

Query: 1682 DLACLLQQTLDYPSSVHGWILVSYLNSKYQPLVVLLPVETI---LKEDYSNPQSDDIYER 1741
             L  LLQ+ +   S V  W+L+S LN + QP+  LLPV++I   + +D +N  S +  + 
Sbjct: 1683 ALNQLLQELMHIRSPVCAWVLISRLNPESQPVATLLPVDSIVEDMSDDSANLSSTEATQV 1742

Query: 1742 NDLNKHWKCPWGSSVIDEVAPAFRIILQENYLSSSVFPTEDTKANRMLWWKRRTKLDDCL 1801
              L   W CPWG++V+DEVAPAF+ IL+E++ SSS    EDT  +R LWWK+R KL+  L
Sbjct: 1743 KSLKGPWLCPWGTTVVDEVAPAFKSILEESHSSSST-TEEDTIESRGLWWKKRKKLNHRL 1802

Query: 1802 GKLLGTIEDSWLGPWKHMLLGELTNRKHADSVLNTLVLDLRSKCKMDVNESLLKVILEGP 1861
            G  L  +E SWLGPW+ +LLGE +N K  DS    LV DL+SKCKM+VNE LLKVIL G 
Sbjct: 1803 GIFLRNLEASWLGPWRCLLLGEWSNYKLPDSAQKKLVNDLKSKCKMEVNEMLLKVILGGG 1862

Query: 1862 EEVL--EAFGSNLNSRKGCFVGKVG-LHDKQRSDPFQNTFNGVDQLSALALKLIQDAKKE 1921
             +    EA  + L+ R GC+VG+ G L+++          N  +    LALKLI DA  +
Sbjct: 1863 TDNFKGEACVAQLSLRNGCYVGRGGYLYEEDSCKTPTAASNISESRHELALKLIHDAASK 1922

Query: 1922 LEGED-NSSREPIIFVLDYDVQMLPWENIPILQNQEVYRMPSVGSICATLDRRCRQQEQV 1981
            L  +D + +REPII VLD +VQMLPWENIPIL+ QEVYRMPSVG I A L +R  Q E  
Sbjct: 1923 LGQQDGHENREPIILVLDPEVQMLPWENIPILRKQEVYRMPSVGCISAVLKKRSLQGEPA 1982

Query: 1982 SGIIPAFPSIDPFDSFYLLNPSGDLRNTQIEFENWFKDQNLEGKAGYAPTSAELIEELKS 2041
               + +FP IDP DSFYLLNP GDL +TQ+ FE+WF+DQN EGKAG  P++ EL E L++
Sbjct: 1983 KSHVASFPLIDPLDSFYLLNPGGDLTDTQVTFESWFRDQNFEGKAGSEPSAIELTEALET 2042

Query: 2042 RDLFIYFGHGSGAQYIPRHEIQKLDVCAASLLMGCSSGSLTLNGYYVPQGVPLSYLKAGS 2101
             DLF+YFGHGSGAQYIPR EI+KLD C+A+ LMGCSSGSL L G Y+PQGVPLSYL  GS
Sbjct: 2043 HDLFLYFGHGSGAQYIPRREIEKLDNCSATFLMGCSSGSLWLKGCYIPQGVPLSYLLGGS 2102

Query: 2102 PVIVANLWEVTDKDIDRFGKAVLEAWLRER--SSVLPSSPRCDILTKEFETINISSKRIS 2161
            P IVA LW+VTD+DIDRFGKA+LEAWL+ER  SS      +C+ L  +   + +   + S
Sbjct: 2103 PAIVATLWDVTDRDIDRFGKALLEAWLQERSDSSSEGGCSQCESLANDLAAMTLKGTKRS 2162

Query: 2162 KKVASKNPPAACESDSSSR--GRPVTSRMIGSFLYEAREACNLRYLIGASPVCYGVPTSI 2178
            +K +S+N PA  + D S +        R IGSF+  AR+ACNL+YLIGA+PVCYGVPT I
Sbjct: 2163 RKPSSRNKPAQSDVDGSGKIECNHKHRRKIGSFIAAARDACNLQYLIGAAPVCYGVPTGI 2171

BLAST of MC10g0420 vs. TAIR 10
Match: AT5G28550.1 (BEST Arabidopsis thaliana protein match is: homolog of separase (TAIR:AT4G22970.2); Has 25 Blast hits to 25 proteins in 6 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 25; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 124.0 bits (310), Expect = 1.5e-27
Identity = 67/174 (38.51%), Postives = 101/174 (58.05%), Query Frame = 0

Query: 532 LLSIVVAAFTLSFRTKLNM-------------KRSTYLIKEIITSKWVQPLALKHLFASL 591
           LL++V+AAF  S RT  N+             K +  L++++I S W+ P  LK LFAS 
Sbjct: 80  LLNVVMAAFIFSMRTLRNVDVKRLLAIVTNQWKITNRLVEDVIASPWISPPELKFLFASF 139

Query: 592 NNIGIILYRHKQIKEASKALKLCCRASWTCVIHFCEKFDDKSRPSDNEFSEDAVLSFVNE 651
           ++I +  Y +K +++AS   KLC R  WTCV   C+ + +KS  S++   ++A++ FV+E
Sbjct: 140 HDISVAFYNNKHLEKASMVFKLCIRTVWTCVRLLCQIYVNKSDLSEDCLPKEAIIDFVSE 199

Query: 652 MIMRSAFLVDILYQCDIYKIEKTMAKILENWSIAAKLFNELPIPVQLVKQWVKM 693
              +SAF +D L+Q    + EK    ILENW  A  L  +LP P  +VKQWVK+
Sbjct: 200 ACSKSAFYLDNLHQHGARETEKLHVFILENWPEAEDLIKKLPDPTPIVKQWVKL 253

BLAST of MC10g0420 vs. TAIR 10
Match: AT4G24300.1 (Peptidase C50, separase )

HSP 1 Score: 64.7 bits (156), Expect = 1.1e-09
Identity = 39/141 (27.66%), Postives = 71/141 (50.35%), Query Frame = 0

Query: 184 VVVTIVKCTASGRSKDSGDYSRVLGLVEEVKPWFRLLDDKVSEKTHRALVTYLNKCTIFL 243
           VV +IV+  A  R  +   Y R + L++ +KPW R LD     K  + +++ + +C + +
Sbjct: 2   VVASIVRSVAMSRDMNDLQYLRGISLLQGLKPWLRYLDANEHHKFLKLVLSDMGECALSV 61

Query: 244 VEELACFGGSLTSLFCRTTLAEYAKSSL-RDQIYKVARRICSTLFSQHLKEDS---SMLI 303
           + E   F  +    FC +TL EY+ S L +   YK +R++ S LF     + S    +++
Sbjct: 62  IREAERFDEAFVHSFCISTLEEYSVSPLPKCHFYKFSRQVLSLLFPSKEAKTSLNLKIMM 121

Query: 304 CVLKSITLELKEEIGCTGVEF 321
            VLK +  E + +   + V +
Sbjct: 122 SVLKFVAGEFQNQQSSSRVRY 142