MC10g0359 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g0359
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Trypsin inhibitor 1-like
Location	MC10: 2886587 .. 2886826 (-)
RNA-Seq Expression	MC10g0359
Synteny	MC10g0359

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAGCAAGAAGGTTGTTGTTGTTGTGGCAATGGTGGTGATGATGCTGGTGGCAACGTCGTCAGCCGCCTTCAACGACGGTCGAGCTGAAACGGGTGAAGTTAATTATCCAAGAAAAATGTTTATAAAAATTGGGGTGTATAATGAGGATGAAAGAATCTGTCCCCTAATCTGGATGGAATGCAAACGCGACTCCGATTGCCTGGCTCAGTGTATTTGCGTGGATGGGCATTGCGGT

mRNA sequence

ATGGAGAGCAAGAAGGTTGTTGTTGTTGTGGCAATGGTGGTGATGATGCTGGTGGCAACGTCGTCAGCCGCCTTCAACGACGGTCGAGCTGAAACGGGTGAAGTTAATTATCCAAGAAAAATGTTTATAAAAATTGGGGTGTATAATGAGGATGAAAGAATCTGTCCCCTAATCTGGATGGAATGCAAACGCGACTCCGATTGCCTGGCTCAGTGTATTTGCGTGGATGGGCATTGCGGT

Coding sequence (CDS)

ATGGAGAGCAAGAAGGTTGTTGTTGTTGTGGCAATGGTGGTGATGATGCTGGTGGCAACGTCGTCAGCCGCCTTCAACGACGGTCGAGCTGAAACGGGTGAAGTTAATTATCCAAGAAAAATGTTTATAAAAATTGGGGTGTATAATGAGGATGAAAGAATCTGTCCCCTAATCTGGATGGAATGCAAACGCGACTCCGATTGCCTGGCTCAGTGTATTTGCGTGGATGGGCATTGCGGT

Protein sequence

MESKKVVVVVAMVVMMLVATSSAAFNDGRAETGEVNYPRKMFIKIGVYNEDERICPLIWMECKRDSDCLAQCICVDGHCG

Homology

[Close]

BLAST of MC10g0359 vs. ExPASy Swiss-Prot
Match: P10296 (Elastase inhibitor 4 OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 4.0e-13
Identity = 30/31 (96.77%), Postives = 31/31 (100.00%), Query Frame = 0


Query: 50 EDERICPLIWMECKRDSDCLAQCICVDGHCG 81
          E+ERICPLIWMECKRDSDCLAQCICVDGHCG
Sbjct: 1  EEERICPLIWMECKRDSDCLAQCICVDGHCG 31

[Close]

BLAST of MC10g0359 vs. ExPASy Swiss-Prot
Match: P10295 (Trypsin inhibitor 2 OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 8.4e-11
Identity = 26/28 (92.86%), Postives = 27/28 (96.43%), Query Frame = 0


Query: 53 RICPLIWMECKRDSDCLAQCICVDGHCG 81
          RICP IWMECKRDSDC+AQCICVDGHCG
Sbjct: 1  RICPRIWMECKRDSDCMAQCICVDGHCG 28

[Close]

BLAST of MC10g0359 vs. ExPASy Swiss-Prot
Match: P30709 (Trypsin inhibitor A OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 5.1e-08
Identity = 22/28 (78.57%), Postives = 24/28 (85.71%), Query Frame = 0


Query: 53 RICPLIWMECKRDSDCLAQCICVDGHCG 81
          R CP IWMEC RDSDC+A+CICV GHCG
Sbjct: 1  RSCPRIWMECTRDSDCMAKCICVAGHCG 28

[Close]

BLAST of MC10g0359 vs. ExPASy Swiss-Prot
Match: P10293 (Trypsin inhibitor 3 OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 1.3e-06
Identity = 21/31 (67.74%), Postives = 28/31 (90.32%), Query Frame = 0


Query: 51 DERICPLIWMECKRDSDCLAQCICVD-GHCG 81
          +ER+CP I MECK+DSDCLA+CIC++ G+CG
Sbjct: 2  EERVCPKILMECKKDSDCLAECICLEHGYCG 32

[Close]

BLAST of MC10g0359 vs. ExPASy Swiss-Prot
Match: P07853 (Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2)

HSP 1 Score: 51.6 bits (122), Expect = 4.8e-06
Identity = 19/31 (61.29%), Postives = 28/31 (90.32%), Query Frame = 0


Query: 51 DERICPLIWMECKRDSDCLAQCICVD-GHCG 81
          +ER+CP I M+CK+DSDCLA+C+C++ G+CG
Sbjct: 2  EERVCPRILMKCKKDSDCLAECVCLEHGYCG 32

[Close]

BLAST of MC10g0359 vs. NCBI nr
Match: P10296.2 (RecName: Full=Elastase inhibitor 4; AltName: Full=Elastase inhibitor IV; AltName: Full=MCEI-IV; Contains: RecName: Full=Elastase inhibitor 3; AltName: Full=Elastase inhibitor III; AltName: Full=MCEI-III; Contains: RecName: Full=Elastase inhibitor 2; AltName: Full=Elastase inhibitor II; AltName: Full=MCEI-II; Contains: RecName: Full=Elastase inhibitor 1; AltName: Full=Elastase inhibitor I; AltName: Full=MCEI-I [Momordica charantia] >AAB34881.1 elastase inhibitor IV, MCEI-IV=serine proteinase inhibitor [Momordica charantia=bitter gourds, LINN, Peptide, 31 aa] [Momordica charantia] >prf||2105265B elastase inhibitor MCEI-IV [Momordica charantia])

HSP 1 Score: 74.7 bits (182), Expect = 5.83e-16
Identity = 30/31 (96.77%), Postives = 31/31 (100.00%), Query Frame = 0


Query: 50 EDERICPLIWMECKRDSDCLAQCICVDGHCG 80
          E+ERICPLIWMECKRDSDCLAQCICVDGHCG
Sbjct: 1  EEERICPLIWMECKRDSDCLAQCICVDGHCG 31

[Close]

BLAST of MC10g0359 vs. NCBI nr
Match: AAB34880.1 (elastase inhibitor III, MCEI-III=serine proteinase inhibitor [Momordica charantia=bitter gourds, LINN, Peptide, 30 aa] [Momordica charantia])

HSP 1 Score: 72.8 bits (177), Expect = 3.33e-15
Identity = 29/30 (96.67%), Postives = 30/30 (100.00%), Query Frame = 0


Query: 51 DERICPLIWMECKRDSDCLAQCICVDGHCG 80
          +ERICPLIWMECKRDSDCLAQCICVDGHCG
Sbjct: 1  EERICPLIWMECKRDSDCLAQCICVDGHCG 30

[Close]

BLAST of MC10g0359 vs. NCBI nr
Match: AAB34879.1 (elastase inhibitor II, MCEI-II=serine proteinase inhibitor [Momordica charantia=bitter gourds, LINN, Peptide, 29 aa] [Momordica charantia])

HSP 1 Score: 72.0 bits (175), Expect = 6.63e-15
Identity = 29/29 (100.00%), Postives = 29/29 (100.00%), Query Frame = 0


Query: 52 ERICPLIWMECKRDSDCLAQCICVDGHCG 80
          ERICPLIWMECKRDSDCLAQCICVDGHCG
Sbjct: 1  ERICPLIWMECKRDSDCLAQCICVDGHCG 29

[Close]

BLAST of MC10g0359 vs. NCBI nr
Match: KAA0044339.1 (trypsin inhibitor 1-like [Cucumis melo var. makuwa] >TYK29468.1 trypsin inhibitor 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 72.0 bits (175), Expect = 2.80e-14
Identity = 42/94 (44.68%), Postives = 60/94 (63.83%), Query Frame = 0


Query: 1  MESKKVVVV--VAMVVMMLVATSSAAF-----------NDGRAETGEVNYPRKMFIKIGV 60
          M+SKK+VVV  V M++M+   +SSA+F           +DGR        PRK+ IK GV
Sbjct: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNN--GCPRKIMIKGGV 60

Query: 61 YNEDERICPLIWMECKRDSDCLAQCICV-DGHCG 80
          + E++R+CP I M+CK+DSDCL  C+C+ +G CG
Sbjct: 61 FKEEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 92

[Close]

BLAST of MC10g0359 vs. NCBI nr
Match: prf||1503172C (Ser protease inhibitor MCEI-I [Momordica charantia])

HSP 1 Score: 70.1 bits (170), Expect = 3.79e-14
Identity = 28/28 (100.00%), Postives = 28/28 (100.00%), Query Frame = 0


Query: 53 RICPLIWMECKRDSDCLAQCICVDGHCG 80
          RICPLIWMECKRDSDCLAQCICVDGHCG
Sbjct: 1  RICPLIWMECKRDSDCLAQCICVDGHCG 28

[Close]

BLAST of MC10g0359 vs. ExPASy TrEMBL
Match: A0A5D3E148 (Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00640 PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.35e-14
Identity = 42/94 (44.68%), Postives = 60/94 (63.83%), Query Frame = 0


Query: 1  MESKKVVVV--VAMVVMMLVATSSAAF-----------NDGRAETGEVNYPRKMFIKIGV 60
          M+SKK+VVV  V M++M+   +SSA+F           +DGR        PRK+ IK GV
Sbjct: 1  MDSKKIVVVAIVGMIIMLAAMSSSASFVVDDKDSTNLVSDGRDMNN--GCPRKIMIKGGV 60

Query: 61 YNEDERICPLIWMECKRDSDCLAQCICV-DGHCG 80
          + E++R+CP I M+CK+DSDCL  C+C+ +G CG
Sbjct: 61 FKEEQRMCPKILMKCKQDSDCLLDCVCLKEGFCG 92

[Close]

BLAST of MC10g0359 vs. ExPASy TrEMBL
Match: A0A0A0KV23 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000835 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.82e-13
Identity = 42/98 (42.86%), Postives = 61/98 (62.24%), Query Frame = 0


Query: 1  MESKKVVVV--VAMVVMMLVATSSAAF-----------NDGRAETGEV---NYPRKMFIK 60
          MESKK+V+V  VA +++ +  +SSA+F           +DGRA+  +      PRKM IK
Sbjct: 1  MESKKIVMVAIVATIIVQVAMSSSASFVVDDKDWIKLVSDGRAQADQDMNNGCPRKMMIK 60

Query: 61 IGVYNEDERICPLIWMECKRDSDCLAQCICVDG--HCG 80
           GV+ E+E +CP I M+CK DSDCL  C+C++   +CG
Sbjct: 61 GGVFKEEEMMCPRILMKCKHDSDCLPGCVCLEHIEYCG 98

[Close]

BLAST of MC10g0359 vs. ExPASy TrEMBL
Match: A6N2U8 (Trypsin inhibitor 2 (Fragment) OS=Momordica charantia OX=3673 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.04e-13
Identity = 26/28 (92.86%), Postives = 27/28 (96.43%), Query Frame = 0


Query: 53 RICPLIWMECKRDSDCLAQCICVDGHCG 80
          RICP IWMECKRDSDC+AQCICVDGHCG
Sbjct: 1  RICPRIWMECKRDSDCMAQCICVDGHCG 28

[Close]

BLAST of MC10g0359 vs. ExPASy TrEMBL
Match: A0A0A7HG47 (TIPRE1 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE1 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.56e-09
Identity = 38/81 (46.91%), Postives = 49/81 (60.49%), Query Frame = 0


Query: 11 AMVVMMLVATSSAAFN---------DGRAETGE-VNYPRKMFIKIGVYNEDERICPLIWM 70
          A+V MMLVATS A FN         +GRA+TG+ +N         GVY+E++R CP I  
Sbjct: 3  ALVAMMLVATS-ADFNGGDTIHLISNGRAQTGQDINSG-------GVYSEEQRACPKILK 62

Query: 71 ECKRDSDCLAQCICVD-GHCG 80
           C+RDSDC   C+C D G+CG
Sbjct: 63 RCRRDSDCPGACVCQDNGYCG 75

[Close]

BLAST of MC10g0359 vs. ExPASy TrEMBL
Match: A0A0A7HIA9 (TIPRE5 (Fragment) OS=Momordica friesiorum OX=703365 GN=TIPRE5 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.56e-09
Identity = 35/80 (43.75%), Postives = 49/80 (61.25%), Query Frame = 0


Query: 11 AMVVMMLVATSSAA--------FNDGRAETGE-VNYPRKMFIKIGVYNEDERICPLIWME 70
          A+V MMLVATS+ +         ++GRA+TG+ +N         GVY+E++R CP I   
Sbjct: 3  ALVAMMLVATSADSNGGDTIHLISNGRAQTGQDINSG-------GVYSEEQRACPKILKR 62

Query: 71 CKRDSDCLAQCICVD-GHCG 80
          C+RDSDC   C+C D G+CG
Sbjct: 63 CRRDSDCPGACVCQDNGYCG 75

The following BLAST results are available for this feature:

BLAST of MC10g0359 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P10296	4.0e-13	96.77	Elastase inhibitor 4 OS=Momordica charantia OX=3673 PE=1 SV=2	[more]
P10295	8.4e-11	92.86	Trypsin inhibitor 2 OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P30709	5.1e-08	78.57	Trypsin inhibitor A OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P10293	1.3e-06	67.74	Trypsin inhibitor 3 OS=Cucurbita pepo OX=3663 PE=1 SV=1	[more]
P07853	4.8e-06	61.29	Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2	[more]

BLAST of MC10g0359 vs. NCBI nr


Match Name	E-value	Identity	Description
P10296.2	5.83e-16	96.77	RecName: Full=Elastase inhibitor 4; AltName: Full=Elastase inhibitor IV; AltName...	[more]
AAB34880.1	3.33e-15	96.67	elastase inhibitor III, MCEI-III=serine proteinase inhibitor [Momordica charanti...	[more]
AAB34879.1	6.63e-15	100.00	elastase inhibitor II, MCEI-II=serine proteinase inhibitor [Momordica charantia=...	[more]
KAA0044339.1	2.80e-14	44.68	trypsin inhibitor 1-like [Cucumis melo var. makuwa] >TYK29468.1 trypsin inhibito...	[more]
prf\|\|1503172C	3.79e-14	100.00	Ser protease inhibitor MCEI-I [Momordica charantia]	[more]

BLAST of MC10g0359 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E148	1.35e-14	44.68	Trypsin inhibitor 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A0A0KV23	1.82e-13	42.86	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000835 PE=3 SV=1	[more]
A6N2U8	3.04e-13	92.86	Trypsin inhibitor 2 (Fragment) OS=Momordica charantia OX=3673 PE=3 SV=1	[more]
A0A0A7HG47	2.56e-09	46.91	TIPRE1 (Fragment) OS=Momordica anigosantha OX=703354 GN=TIPRE1 PE=3 SV=1	[more]
A0A0A7HIA9	2.56e-09	43.75	TIPRE5 (Fragment) OS=Momordica friesiorum OX=703365 GN=TIPRE5 PE=3 SV=1	[more]

BLAST of MC10g0359 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00286	PTI_1	coord: 53..80 e-value: 3.8E-11 score: 53.0
None	No IPR available	GENE3D	4.10.75.20		coord: 30..80 e-value: 3.2E-12 score: 48.1
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 53..80 e-value: 3.0E-15 score: 55.9
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 55..74
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 55..80 e-value: 4.31607E-8 score: 42.7499
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	57027	Plant inhibitors of proteinases and amylases	coord: 50..80

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g0359.1	MC10g0359.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity