MC10g0335 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0335
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionTubulin alpha chain
LocationMC10: 2691236 .. 2693571 (-)
RNA-Seq ExpressionMC10g0335
SyntenyMC10g0335
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGAGTGCATCTCCATCCACATCGGTCAGGCCGGTATCCAGGTCGGAAATGCTTGCTGGGAGCTCTACTGCCTCGAGCACGGCATTCAGGTATTCTACGCTCGTTTCTTTGCCTTTCTTTCTCTCGATCTGTAAGGAATTGTCGTCATTTGTGCATCTAGTGATGTTTTGGCTTCCGTTCTGTTGTTTTTGTTAGATTTGTAAGAGATTTGTCGTCTGCCTTTCATTTGCTCGATCTGTATGGAATTATTGGCATTTCTGCATCTTGCGTTGTTGTGGCATAGGTTTCATTGTCTTTGTTCTGGCTCTCGTTTGCCCGATCTGTAAGGAATTATCATCATTTCTGAATCCAGCGATTTTGTCGCATAGGTTTTGTTGTTCTTGTTAGATTTAGAAGCTTTACCGCACGCATCGAGTGTTTTTATGTGGATCAGTACGACTTGTTTGATTAACTCCACGTTTTTCCCATATTGCATGATGTGTTATTGAGGTTACACGCTGTGTCTCGTTAGATCTACATCATCAATCTGTGTTGGAAAATCGTAAATTGTTGAATCTGTGTTGTATTCCATCGTTAGTCCACCTTGTCTTCGAGATATGGAATATTTGTCCTTCTGTCCATCGAGATCTGGAAATAGAGAACGTATCTAGCTGCGATCTGTTGAATCTCAGGCGTGTGTTGAATTTCTAGTTGAATACCTTTGGATCTTCGATTTGTAATGTGCGTGGTTAAAGTCGCTGTTTATTTGAAATCTCTGTTATTACATTCTCCTCGTTATCTGCATTTTGCCTTATCTCTGAATAATTGGCCTATTTATATTTGATTTAGAACCAATCGAATGCTGTTAGGAAAATGCCACCCAGGTGAAGCGAACAAAGTGATCTAAATCTTGTTTGTAAATCTGTGCAGCCCGATGGCCAAATGCCCGGTGACACCACTCTTGGTGGAGGCGACGATGCTTTCAACACCTTTTTCAGTGAAACAGGTGCAGGAAAGCATGTTCCTCGTGCAGTGTTTGTTGATCTTGAGCCTACTGTCATCGATGAGGTGAGGACTGGAACATACCGCCAGCTCTTCCACCCTGAACAGCTCATCAGTGGCAAAGAAGATGCCGCCAATAATTTCGCCCGTGGTCACTACACCGGTAATTACTTGATCTAGATTTCTCCAATGAATTAGATCCAATATTCCTTTATTCATAATGTTTTCTCTGTTTAATTTCAGTTGGGAAGGAAATTGTTGATCTTTGCTTGGACCGAATCCGTAAGCTGGCAGACAACTGCACTGGTCTTCAAGGATTTCTTGTGTTTAATGCTGTTGGTGGTGGTACTGGCTCTGGTCTTGGCTCCCTCCTTTTGGAGCGCTTGTCGGTTGACTATGGAAAGAAATCCAAGCTCGGGTTCACTGTTTACCCATCCCCACAAGTCTCAACTTCTGTTGTTGAGCCCTACAACAGTGTCCTTTCAACCCACTCTCTCTTGGAACACACCGATGTTGCTGTGCTCCTCGACAATGAAGCCATCTATGATATCTGCAGGCGCTCCCTTGACATCGAGCGACCCAACTACTCCAACCTCAACCGTCTCGTGTCCCAGGTAACCATTTAAGAGTTTTTGGATCAGATCTGATACCAATAAAACTATGAACTTCTGGGAGGCTCACATGATTTTGTATAATTCAGGTTATATCATCTTTAACCGCCAGTCTGAGGTTCGATGGTGCCCTGAACGTGGATGTGAACGAATTCCAGACCAACTTGGTCCCCTACCCCAGAATCCACTTTATGCTTTCCTCCTATGCACCAGTGATCTCAGCCGAGAAGGCCTACCACGAGCAACTCTCAGTGGCCGAAATCACCAACAGTGCCTTCGAGCCGTCGTCCATGATGGTTAAGTGCGACCCCCGACACGGCAAGTACATGGCATGCTGCCTTATGTACCGAGGTGATGTTGTGCCCAAGGACGTGAATGCTGCTGTTGCCACCATCAAGACCAAGCGCACCATCCAGTTTGTTGACTGGTGCCCCACTGGGTTCAAGTGTGGTATCAACTACCAACCACCAACTGTTGTCCCCGGAGGCGATCTGGCCCGTGTGCAGAGGGCTGTTTGCAAGATCTCGAACTCCACCAGTGTTGCTGAGGTCTTCTCTCGCATCGACCACAAGTTCGATCTCATGTATGCCAAACGTGCCTTTGTGCACTGGTATGTCGGTGAGGGTATGGAGGAAGGAGAGTTCTCCGAGGCTCGGGAGGATCTTGCTGCCCTCGAGAAGGATTACGAAGAAGTCGGTGCTGAGTCAGCCGAGGGTGAAGATGATGAAGGTGAGGATTAT

mRNA sequence

ATGAGAGAGTGCATCTCCATCCACATCGGTCAGGCCGGTATCCAGGTCGGAAATGCTTGCTGGGAGCTCTACTGCCTCGAGCACGGCATTCAGCCCGATGGCCAAATGCCCGGTGACACCACTCTTGGTGGAGGCGACGATGCTTTCAACACCTTTTTCAGTGAAACAGGTGCAGGAAAGCATGTTCCTCGTGCAGTGTTTGTTGATCTTGAGCCTACTGTCATCGATGAGGTGAGGACTGGAACATACCGCCAGCTCTTCCACCCTGAACAGCTCATCAGTGGCAAAGAAGATGCCGCCAATAATTTCGCCCGTGGTCACTACACCGTTGGGAAGGAAATTGTTGATCTTTGCTTGGACCGAATCCGTAAGCTGGCAGACAACTGCACTGGTCTTCAAGGATTTCTTGTGTTTAATGCTGTTGGTGGTGGTACTGGCTCTGGTCTTGGCTCCCTCCTTTTGGAGCGCTTGTCGGTTGACTATGGAAAGAAATCCAAGCTCGGGTTCACTGTTTACCCATCCCCACAAGTCTCAACTTCTGTTGTTGAGCCCTACAACAGTGTCCTTTCAACCCACTCTCTCTTGGAACACACCGATGTTGCTGTGCTCCTCGACAATGAAGCCATCTATGATATCTGCAGGCGCTCCCTTGACATCGAGCGACCCAACTACTCCAACCTCAACCGTCTCGTGTCCCAGGTTATATCATCTTTAACCGCCAGTCTGAGGTTCGATGGTGCCCTGAACGTGGATGTGAACGAATTCCAGACCAACTTGGTCCCCTACCCCAGAATCCACTTTATGCTTTCCTCCTATGCACCAGTGATCTCAGCCGAGAAGGCCTACCACGAGCAACTCTCAGTGGCCGAAATCACCAACAGTGCCTTCGAGCCGTCGTCCATGATGGTTAAGTGCGACCCCCGACACGGCAAGTACATGGCATGCTGCCTTATGTACCGAGGTGATGTTGTGCCCAAGGACGTGAATGCTGCTGTTGCCACCATCAAGACCAAGCGCACCATCCAGTTTGTTGACTGGTGCCCCACTGGGTTCAAGTGTGGTATCAACTACCAACCACCAACTGTTGTCCCCGGAGGCGATCTGGCCCGTGTGCAGAGGGCTGTTTGCAAGATCTCGAACTCCACCAGTGTTGCTGAGGTCTTCTCTCGCATCGACCACAAGTTCGATCTCATGTATGCCAAACGTGCCTTTGTGCACTGGTATGTCGGTGAGGGTATGGAGGAAGGAGAGTTCTCCGAGGCTCGGGAGGATCTTGCTGCCCTCGAGAAGGATTACGAAGAAGTCGGTGCTGAGTCAGCCGAGGGTGAAGATGATGAAGGTGAGGATTAT

Coding sequence (CDS)

ATGAGAGAGTGCATCTCCATCCACATCGGTCAGGCCGGTATCCAGGTCGGAAATGCTTGCTGGGAGCTCTACTGCCTCGAGCACGGCATTCAGCCCGATGGCCAAATGCCCGGTGACACCACTCTTGGTGGAGGCGACGATGCTTTCAACACCTTTTTCAGTGAAACAGGTGCAGGAAAGCATGTTCCTCGTGCAGTGTTTGTTGATCTTGAGCCTACTGTCATCGATGAGGTGAGGACTGGAACATACCGCCAGCTCTTCCACCCTGAACAGCTCATCAGTGGCAAAGAAGATGCCGCCAATAATTTCGCCCGTGGTCACTACACCGTTGGGAAGGAAATTGTTGATCTTTGCTTGGACCGAATCCGTAAGCTGGCAGACAACTGCACTGGTCTTCAAGGATTTCTTGTGTTTAATGCTGTTGGTGGTGGTACTGGCTCTGGTCTTGGCTCCCTCCTTTTGGAGCGCTTGTCGGTTGACTATGGAAAGAAATCCAAGCTCGGGTTCACTGTTTACCCATCCCCACAAGTCTCAACTTCTGTTGTTGAGCCCTACAACAGTGTCCTTTCAACCCACTCTCTCTTGGAACACACCGATGTTGCTGTGCTCCTCGACAATGAAGCCATCTATGATATCTGCAGGCGCTCCCTTGACATCGAGCGACCCAACTACTCCAACCTCAACCGTCTCGTGTCCCAGGTTATATCATCTTTAACCGCCAGTCTGAGGTTCGATGGTGCCCTGAACGTGGATGTGAACGAATTCCAGACCAACTTGGTCCCCTACCCCAGAATCCACTTTATGCTTTCCTCCTATGCACCAGTGATCTCAGCCGAGAAGGCCTACCACGAGCAACTCTCAGTGGCCGAAATCACCAACAGTGCCTTCGAGCCGTCGTCCATGATGGTTAAGTGCGACCCCCGACACGGCAAGTACATGGCATGCTGCCTTATGTACCGAGGTGATGTTGTGCCCAAGGACGTGAATGCTGCTGTTGCCACCATCAAGACCAAGCGCACCATCCAGTTTGTTGACTGGTGCCCCACTGGGTTCAAGTGTGGTATCAACTACCAACCACCAACTGTTGTCCCCGGAGGCGATCTGGCCCGTGTGCAGAGGGCTGTTTGCAAGATCTCGAACTCCACCAGTGTTGCTGAGGTCTTCTCTCGCATCGACCACAAGTTCGATCTCATGTATGCCAAACGTGCCTTTGTGCACTGGTATGTCGGTGAGGGTATGGAGGAAGGAGAGTTCTCCGAGGCTCGGGAGGATCTTGCTGCCCTCGAGAAGGATTACGAAGAAGTCGGTGCTGAGTCAGCCGAGGGTGAAGATGATGAAGGTGAGGATTAT

Protein sequence

MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLDRIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTSVVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTASLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSSMMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPPTVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAESAEGEDDEGEDY
Homology
BLAST of MC10g0335 vs. ExPASy Swiss-Prot
Match: P33629 (Tubulin alpha chain OS=Prunus dulcis OX=3755 GN=TUBA PE=2 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 1.6e-260
Identity = 440/450 (97.78%), Postives = 445/450 (98.89%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGD T+GGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYT+GKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERP Y+NLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDV EFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MM KCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRAVC ISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 451
           AREDLAALEKDYEEVGAESAEGEDDEG+DY
Sbjct: 421 AREDLAALEKDYEEVGAESAEGEDDEGDDY 450

BLAST of MC10g0335 vs. ExPASy Swiss-Prot
Match: Q9ZRR5 (Tubulin alpha-3 chain OS=Hordeum vulgare OX=4513 GN=TUBA3 PE=2 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 4.9e-257
Identity = 436/451 (96.67%), Postives = 443/451 (98.23%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGD T+GGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYT+GKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERP Y+NLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS
Sbjct: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MM KCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           +VVPGGDLA+VQRAVC ISNSTSV EVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 SVVPGGDLAKVQRAVCMISNSTSVVEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGED-DEGEDY 451
           AREDLAALEKDYEEVGAE  EGED DEG++Y
Sbjct: 421 AREDLAALEKDYEEVGAEFDEGEDGDEGDEY 451

BLAST of MC10g0335 vs. ExPASy Swiss-Prot
Match: P46259 (Tubulin alpha-1 chain OS=Pisum sativum OX=3888 GN=TUBA1 PE=3 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 3.2e-256
Identity = 432/445 (97.08%), Postives = 438/445 (98.43%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGD T+GGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTG YRQLFHPEQLISGKEDAANNFARGHYT+GKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGAYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERP Y+NLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDV EFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MM KCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRAVC ISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDD 446
           AREDLAALEKDYEEVGAES +G+DD
Sbjct: 421 AREDLAALEKDYEEVGAESGDGDDD 445

BLAST of MC10g0335 vs. ExPASy Swiss-Prot
Match: P14640 (Tubulin alpha-1 chain OS=Zea mays OX=4577 GN=TUBA1 PE=3 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 4.1e-256
Identity = 434/451 (96.23%), Postives = 442/451 (98.00%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQ DGQMPGD T+GGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQADGQMPGDKTIGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYT+GKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDVA+LLDNEAIYDICRRSLDIERP Y+NLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAILLDNEAIYDICRRSLDIERPTYTNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS
Sbjct: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MM KCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           +VVPGGDLA+VQRAVC ISNSTSV EVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 SVVPGGDLAKVQRAVCMISNSTSVVEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGED-DEGEDY 451
           AREDLAALEKDYEEVGAE  EGED DEG++Y
Sbjct: 421 AREDLAALEKDYEEVGAEFDEGEDGDEGDEY 451

BLAST of MC10g0335 vs. ExPASy Swiss-Prot
Match: P29511 (Tubulin alpha-6 chain OS=Arabidopsis thaliana OX=3702 GN=TUBA6 PE=1 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 5.4e-256
Identity = 430/450 (95.56%), Postives = 441/450 (98.00%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGD T+GGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYT+GKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDV++LLDNEAIYDICRRSL+IERP Y+NLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSILLDNEAIYDICRRSLNIERPTYTNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDV EFQTNLVPYPRIHFMLSSYAPVISAEKA+HEQLSVAEITNSAFEP+S
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAFHEQLSVAEITNSAFEPAS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MM KCDPRHGKYMACCLMYRGDVVPKDVNAAV TIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVGTIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRAVC ISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 451
           AREDLAALEKDYEEVGAE  + EDDEGE+Y
Sbjct: 421 AREDLAALEKDYEEVGAEGGDDEDDEGEEY 450

BLAST of MC10g0335 vs. NCBI nr
Match: XP_004150268.1 (tubulin alpha chain isoform X1 [Cucumis sativus] >XP_008454421.1 PREDICTED: tubulin alpha chain [Cucumis melo] >XP_022149319.1 tubulin alpha chain-like [Momordica charantia] >XP_038904098.1 tubulin alpha chain [Benincasa hispida] >ADN33817.1 tubulin alpha chain [Cucumis melo subsp. melo] >KAA0044315.1 tubulin alpha chain isoform X1 [Cucumis melo var. makuwa] >KGN52737.1 hypothetical protein Csa_015231 [Cucumis sativus] >TYK29444.1 tubulin alpha chain isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 908 bits (2346), Expect = 0.0
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS
Sbjct: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450
           AREDLAALEKDYEEVGAESAEGEDDEGEDY
Sbjct: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450

BLAST of MC10g0335 vs. NCBI nr
Match: XP_022942294.1 (tubulin alpha chain-like [Cucurbita moschata] >XP_022976046.1 tubulin alpha chain [Cucurbita maxima] >XP_023532274.1 tubulin alpha chain-like [Cucurbita pepo subsp. pepo] >KAG6600270.1 Tubulin alpha chain, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030929.1 Tubulin alpha chain [Cucurbita argyrosperma subsp. argyrosperma] >QBA97745.1 tubulin alpha chain [Cucurbita pepo])

HSP 1 Score: 906 bits (2341), Expect = 0.0
Identity = 449/450 (99.78%), Postives = 449/450 (99.78%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTT GGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTTGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS
Sbjct: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450
           AREDLAALEKDYEEVGAESAEGEDDEGEDY
Sbjct: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450

BLAST of MC10g0335 vs. NCBI nr
Match: XP_022922758.1 (tubulin alpha chain-like [Cucurbita moschata] >KAG6576904.1 Tubulin alpha chain, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014929.1 Tubulin alpha chain [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 905 bits (2339), Expect = 0.0
Identity = 449/450 (99.78%), Postives = 449/450 (99.78%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTT GGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTHGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS
Sbjct: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450
           AREDLAALEKDYEEVGAESAEGEDDEGEDY
Sbjct: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450

BLAST of MC10g0335 vs. NCBI nr
Match: XP_022984449.1 (tubulin alpha chain-like [Cucurbita maxima] >XP_023552263.1 tubulin alpha chain-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 904 bits (2335), Expect = 0.0
Identity = 448/450 (99.56%), Postives = 449/450 (99.78%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGD+T GGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDSTHGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS
Sbjct: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450
           AREDLAALEKDYEEVGAESAEGEDDEGEDY
Sbjct: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450

BLAST of MC10g0335 vs. NCBI nr
Match: XP_034227058.1 (tubulin alpha chain [Prunus dulcis] >P33629.1 RecName: Full=Tubulin alpha chain [Prunus dulcis] >CAA47635.1 alpha-tubulin [Prunus dulcis] >VVA15546.1 PREDICTED: tubulin [Prunus dulcis])

HSP 1 Score: 892 bits (2305), Expect = 0.0
Identity = 440/450 (97.78%), Postives = 445/450 (98.89%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGD T+GGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYT+GKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERP Y+NLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDV EFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MM KCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRAVC ISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450
           AREDLAALEKDYEEVGAESAEGEDDEG+DY
Sbjct: 421 AREDLAALEKDYEEVGAESAEGEDDEGDDY 450

BLAST of MC10g0335 vs. ExPASy TrEMBL
Match: A0A5A7TSF5 (Tubulin alpha chain OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00370 PE=3 SV=1)

HSP 1 Score: 908 bits (2346), Expect = 0.0
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS
Sbjct: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450
           AREDLAALEKDYEEVGAESAEGEDDEGEDY
Sbjct: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450

BLAST of MC10g0335 vs. ExPASy TrEMBL
Match: E5GBH5 (Tubulin alpha chain OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 908 bits (2346), Expect = 0.0
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS
Sbjct: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450
           AREDLAALEKDYEEVGAESAEGEDDEGEDY
Sbjct: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450

BLAST of MC10g0335 vs. ExPASy TrEMBL
Match: A0A0A0KWB5 (Tubulin alpha chain OS=Cucumis sativus OX=3659 GN=Csa_4G000580 PE=3 SV=1)

HSP 1 Score: 908 bits (2346), Expect = 0.0
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS
Sbjct: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450
           AREDLAALEKDYEEVGAESAEGEDDEGEDY
Sbjct: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450

BLAST of MC10g0335 vs. ExPASy TrEMBL
Match: A0A6J1D6Q0 (Tubulin alpha chain OS=Momordica charantia OX=3673 GN=LOC111017762 PE=3 SV=1)

HSP 1 Score: 908 bits (2346), Expect = 0.0
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS
Sbjct: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450
           AREDLAALEKDYEEVGAESAEGEDDEGEDY
Sbjct: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450

BLAST of MC10g0335 vs. ExPASy TrEMBL
Match: A0A1S3BZT0 (Tubulin alpha chain OS=Cucumis melo OX=3656 GN=LOC103494831 PE=3 SV=1)

HSP 1 Score: 908 bits (2346), Expect = 0.0
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS
Sbjct: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450
           AREDLAALEKDYEEVGAESAEGEDDEGEDY
Sbjct: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 450

BLAST of MC10g0335 vs. TAIR 10
Match: AT4G14960.2 (Tubulin/FtsZ family protein )

HSP 1 Score: 884.4 bits (2284), Expect = 3.8e-257
Identity = 430/450 (95.56%), Postives = 441/450 (98.00%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGD T+GGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYT+GKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDV++LLDNEAIYDICRRSL+IERP Y+NLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSILLDNEAIYDICRRSLNIERPTYTNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDV EFQTNLVPYPRIHFMLSSYAPVISAEKA+HEQLSVAEITNSAFEP+S
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAFHEQLSVAEITNSAFEPAS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MM KCDPRHGKYMACCLMYRGDVVPKDVNAAV TIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVGTIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRAVC ISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 451
           AREDLAALEKDYEEVGAE  + EDDEGE+Y
Sbjct: 421 AREDLAALEKDYEEVGAEGGDDEDDEGEEY 450

BLAST of MC10g0335 vs. TAIR 10
Match: AT1G04820.1 (tubulin alpha-4 chain )

HSP 1 Score: 881.7 bits (2277), Expect = 2.5e-256
Identity = 429/450 (95.33%), Postives = 439/450 (97.56%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMP D T+GGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYT+GKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDV++LLDNEAIYDICRRSL IERP Y+NLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSILLDNEAIYDICRRSLSIERPTYTNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDV EFQTNLVPYPRIHFMLSSYAPVISAEKA+HEQLSVAEITNSAFEP+S
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAFHEQLSVAEITNSAFEPAS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MM KCDPRHGKYMACCLMYRGDVVPKDVNAAV TIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVGTIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRAVC ISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 451
           AREDLAALEKDYEEVGAE  + EDDEGE+Y
Sbjct: 421 AREDLAALEKDYEEVGAEGGDDEDDEGEEY 450

BLAST of MC10g0335 vs. TAIR 10
Match: AT1G50010.1 (tubulin alpha-2 chain )

HSP 1 Score: 881.7 bits (2277), Expect = 2.5e-256
Identity = 429/450 (95.33%), Postives = 439/450 (97.56%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMP D T+GGGDDAFNTFFSETGAGK
Sbjct: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPSDKTVGGGDDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYT+GKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTIGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS
Sbjct: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDV++LLDNEAIYDICRRSL IERP Y+NLNRLVSQVISSLTA
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVSILLDNEAIYDICRRSLSIERPTYTNLNRLVSQVISSLTA 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGALNVDV EFQTNLVPYPRIHFMLSSYAPVISAEKA+HEQLSVAEITNSAFEP+S
Sbjct: 241 SLRFDGALNVDVTEFQTNLVPYPRIHFMLSSYAPVISAEKAFHEQLSVAEITNSAFEPAS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MM KCDPRHGKYMACCLMYRGDVVPKDVNAAV TIKTKRTIQFVDWCPTGFKCGINYQPP
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVGTIKTKRTIQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRAVC ISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 451
           AREDLAALEKDYEEVGAE  + EDDEGE+Y
Sbjct: 421 AREDLAALEKDYEEVGAEGGDDEDDEGEEY 450

BLAST of MC10g0335 vs. TAIR 10
Match: AT5G19770.1 (tubulin alpha-3 )

HSP 1 Score: 859.4 bits (2219), Expect = 1.3e-249
Identity = 414/450 (92.00%), Postives = 432/450 (96.00%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRE ISIHIGQAGIQVGN+CWELYCLEHGIQPDG MP DTT+G   DAFNTFFSETGAGK
Sbjct: 1   MREIISIHIGQAGIQVGNSCWELYCLEHGIQPDGMMPSDTTVGVAHDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           R+RKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFT+YPSPQVST+
Sbjct: 121 RVRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTIYPSPQVSTA 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERP Y+NLNRL+SQ+ISSLT 
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLISQIISSLTT 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGA+NVD+ EFQTNLVPYPRIHFMLSSYAPVISA KAYHEQLSV EITN+ FEP+S
Sbjct: 241 SLRFDGAINVDITEFQTNLVPYPRIHFMLSSYAPVISAAKAYHEQLSVPEITNAVFEPAS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MM KCDPRHGKYMACCLMYRGDVVPKDVNAAV TIKTKRT+QFVDWCPTGFKCGINYQPP
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVGTIKTKRTVQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRAVC ISN+T+VAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNNTAVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 451
           AREDLAALEKDYEEVGAE  + E+DEGEDY
Sbjct: 421 AREDLAALEKDYEEVGAEGGDDEEDEGEDY 450

BLAST of MC10g0335 vs. TAIR 10
Match: AT5G19780.1 (tubulin alpha-5 )

HSP 1 Score: 859.4 bits (2219), Expect = 1.3e-249
Identity = 414/450 (92.00%), Postives = 432/450 (96.00%), Query Frame = 0

Query: 1   MRECISIHIGQAGIQVGNACWELYCLEHGIQPDGQMPGDTTLGGGDDAFNTFFSETGAGK 60
           MRE ISIHIGQAGIQVGN+CWELYCLEHGIQPDG MP DTT+G   DAFNTFFSETGAGK
Sbjct: 1   MREIISIHIGQAGIQVGNSCWELYCLEHGIQPDGMMPSDTTVGVAHDAFNTFFSETGAGK 60

Query: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120
           HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD
Sbjct: 61  HVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLISGKEDAANNFARGHYTVGKEIVDLCLD 120

Query: 121 RIRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTVYPSPQVSTS 180
           R+RKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFT+YPSPQVST+
Sbjct: 121 RVRKLADNCTGLQGFLVFNAVGGGTGSGLGSLLLERLSVDYGKKSKLGFTIYPSPQVSTA 180

Query: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPNYSNLNRLVSQVISSLTA 240
           VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERP Y+NLNRL+SQ+ISSLT 
Sbjct: 181 VVEPYNSVLSTHSLLEHTDVAVLLDNEAIYDICRRSLDIERPTYTNLNRLISQIISSLTT 240

Query: 241 SLRFDGALNVDVNEFQTNLVPYPRIHFMLSSYAPVISAEKAYHEQLSVAEITNSAFEPSS 300
           SLRFDGA+NVD+ EFQTNLVPYPRIHFMLSSYAPVISA KAYHEQLSV EITN+ FEP+S
Sbjct: 241 SLRFDGAINVDITEFQTNLVPYPRIHFMLSSYAPVISAAKAYHEQLSVPEITNAVFEPAS 300

Query: 301 MMVKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWCPTGFKCGINYQPP 360
           MM KCDPRHGKYMACCLMYRGDVVPKDVNAAV TIKTKRT+QFVDWCPTGFKCGINYQPP
Sbjct: 301 MMAKCDPRHGKYMACCLMYRGDVVPKDVNAAVGTIKTKRTVQFVDWCPTGFKCGINYQPP 360

Query: 361 TVVPGGDLARVQRAVCKISNSTSVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420
           TVVPGGDLA+VQRAVC ISN+T+VAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
Sbjct: 361 TVVPGGDLAKVQRAVCMISNNTAVAEVFSRIDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 420

Query: 421 AREDLAALEKDYEEVGAESAEGEDDEGEDY 451
           AREDLAALEKDYEEVGAE  + E+DEGEDY
Sbjct: 421 AREDLAALEKDYEEVGAEGGDDEEDEGEDY 450

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P336291.6e-26097.78Tubulin alpha chain OS=Prunus dulcis OX=3755 GN=TUBA PE=2 SV=1[more]
Q9ZRR54.9e-25796.67Tubulin alpha-3 chain OS=Hordeum vulgare OX=4513 GN=TUBA3 PE=2 SV=1[more]
P462593.2e-25697.08Tubulin alpha-1 chain OS=Pisum sativum OX=3888 GN=TUBA1 PE=3 SV=1[more]
P146404.1e-25696.23Tubulin alpha-1 chain OS=Zea mays OX=4577 GN=TUBA1 PE=3 SV=1[more]
P295115.4e-25695.56Tubulin alpha-6 chain OS=Arabidopsis thaliana OX=3702 GN=TUBA6 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_004150268.10.0100.00tubulin alpha chain isoform X1 [Cucumis sativus] >XP_008454421.1 PREDICTED: tubu... [more]
XP_022942294.10.099.78tubulin alpha chain-like [Cucurbita moschata] >XP_022976046.1 tubulin alpha chai... [more]
XP_022922758.10.099.78tubulin alpha chain-like [Cucurbita moschata] >KAG6576904.1 Tubulin alpha chain,... [more]
XP_022984449.10.099.56tubulin alpha chain-like [Cucurbita maxima] >XP_023552263.1 tubulin alpha chain-... [more]
XP_034227058.10.097.78tubulin alpha chain [Prunus dulcis] >P33629.1 RecName: Full=Tubulin alpha chain ... [more]
Match NameE-valueIdentityDescription
A0A5A7TSF50.0100.00Tubulin alpha chain OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G... [more]
E5GBH50.0100.00Tubulin alpha chain OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A0A0KWB50.0100.00Tubulin alpha chain OS=Cucumis sativus OX=3659 GN=Csa_4G000580 PE=3 SV=1[more]
A0A6J1D6Q00.0100.00Tubulin alpha chain OS=Momordica charantia OX=3673 GN=LOC111017762 PE=3 SV=1[more]
A0A1S3BZT00.0100.00Tubulin alpha chain OS=Cucumis melo OX=3656 GN=LOC103494831 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G14960.23.8e-25795.56Tubulin/FtsZ family protein [more]
AT1G04820.12.5e-25695.33tubulin alpha-4 chain [more]
AT1G50010.12.5e-25695.33tubulin alpha-2 chain [more]
AT5G19770.11.3e-24992.00tubulin alpha-3 [more]
AT5G19780.11.3e-24992.00tubulin alpha-5 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 418..438
NoneNo IPR availablePANTHERPTHR11588:SF405TUBULIN ALPHA CHAINcoord: 1..444
NoneNo IPR availableCDDcd02186alpha_tubulincoord: 2..435
e-value: 0.0
score: 894.587
IPR000217TubulinPRINTSPR01161TUBULINcoord: 53..72
score: 68.27
coord: 134..152
score: 66.84
coord: 108..132
score: 59.46
coord: 192..212
score: 61.21
coord: 380..408
score: 57.59
coord: 10..30
score: 66.54
coord: 95..106
score: 64.97
coord: 153..174
score: 49.68
coord: 178..191
score: 70.91
IPR000217TubulinPANTHERPTHR11588TUBULINcoord: 1..444
IPR002452Alpha tubulinPRINTSPR01162ALPHATUBULINcoord: 88..101
score: 94.12
coord: 214..226
score: 75.05
coord: 274..289
score: 88.92
coord: 161..172
score: 83.82
coord: 422..438
score: 90.81
coord: 148..160
score: 86.23
coord: 18..33
score: 95.27
coord: 120..128
score: 86.55
coord: 321..342
score: 90.72
coord: 45..58
score: 85.23
coord: 360..379
score: 79.66
coord: 347..360
score: 93.1
coord: 385..401
score: 83.59
IPR018316Tubulin/FtsZ, 2-layer sandwich domainSMARTSM00865Tubulin_C_4coord: 248..393
e-value: 8.1E-54
score: 194.8
IPR018316Tubulin/FtsZ, 2-layer sandwich domainPFAMPF03953Tubulin_Ccoord: 263..392
e-value: 2.3E-50
score: 170.0
IPR003008Tubulin/FtsZ, GTPase domainSMARTSM00864Tubulin_4coord: 49..246
e-value: 1.5E-72
score: 257.0
IPR003008Tubulin/FtsZ, GTPase domainPFAMPF00091Tubulincoord: 3..213
e-value: 1.1E-67
score: 228.1
IPR036525Tubulin/FtsZ, GTPase domain superfamilyGENE3D3.40.50.1440Tubulin/FtsZ, GTPase domaincoord: 1..268
e-value: 9.9E-132
score: 440.3
IPR036525Tubulin/FtsZ, GTPase domain superfamilySUPERFAMILY52490Tubulin nucleotide-binding domain-likecoord: 1..244
IPR037103Tubulin/FtsZ-like, C-terminal domainGENE3D3.30.1330.20coord: 269..383
e-value: 1.5E-58
score: 197.6
IPR023123Tubulin, C-terminalGENE3D1.10.287.600Helix hairpin bincoord: 384..436
e-value: 1.4E-31
score: 109.9
IPR017975Tubulin, conserved sitePROSITEPS00227TUBULINcoord: 142..148
IPR008280Tubulin/FtsZ, C-terminalSUPERFAMILY55307Tubulin C-terminal domain-likecoord: 246..436

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0335.1MC10g0335.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000226 microtubule cytoskeleton organization
biological_process GO:0000278 mitotic cell cycle
biological_process GO:0007017 microtubule-based process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005874 microtubule
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0005200 structural constituent of cytoskeleton