MC10g0280 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g0280
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Ribonuclease T(2)
Location	MC10: 2309105 .. 2311763 (-)
RNA-Seq Expression	MC10g0280
Synteny	MC10g0280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAAAAATAAAACTATATACATCTTAATTAATGATACGAAGTTAAAGAACAATTCTACCCACCAAAAACAACTCGGATTACTTTGTATAAGTACGAGGATGCATGGAATTTGGAAAGCCAGATTTCAGTAGTACAAAAGCAGATTACTTTCCTATTTTGCGAAAAAAATGGCTTCTTCCTCCATCAAAGGCAACCCCACGGTGGTGGCCACCGTGTTCGTTCTTGTTGTTCTTCAGTTGCAGGCTGCGGCCTTTGCAGCGGGCGCCGCCCCCGGCAGCGAGGAGCAGCGGGAGTTCGACTACTTTGTGTTGGCCTTGCAATGGCCAGCCACTTCCTGCACAAATCCCGGCAAGTGTTGCCCCTCCAACGGTTGCTGTCGCGGGTGAGTTTTATTAATATTCATGCGGTTCCATCTAAAAATCAATTAACAACGGAAGGAATAATGAATGTCTATTAGAAATTTTTATTGTCGGGACTCTTCTGGTTCATTAATCGTGGTCAGATCCTCGTTTAAACGTGTTTTAGACTCAAATACCGTATCGATATTCATAGGTTTTATCTAAAAACTAATTGACTATGAGAAGAGTAAATCATGTCAATTATAAACACTGGTTAAGTCTTTTATCTTTTTCTCGTAATTAACCTTCATCTAGATAAACCTTCATCTTTGGATTTTTTTTCTTTCTTTTGATGGGGTTGGTTTCAGTTGACTTTTTGAAGCTAACGATTGTTTTGTCTGATTTTTTTTCGTGTGTCTGCAGAGCTGAGTCTCCCACTGAATTTACAATCCGTAAGTGGTGTAATTAAATTGATTAATAGTGTTTTAGTTAAATTAATTAATAGTGTTTTAATTATGTGGTGGAATAATTAATTAAATGTGGCAGATGGGCTGTGGCCTCAGTATGAAGAGAAAGGATGGCCTTCATGTTGCAGTGATGCAAGCTTCAGTGAAAATGAGGTCTCTCTCTCTCTCTCACACACACAACAATCATCTCTCTTTTCAAACATAACTTAAGTGTTTGCTCGTCTTATTTTATACTACATTTAAATTTGATTTCGACTTAAATTATGCTAGTTTTGTAAAACGAATTAAAGGTGAACAAAATGTATCATTTCCAATTTGTTTTCATACTTATAAAACGAACAAATCGTTAAAACGAGTCTAACTCAACGATAATTGACATATATCTCTCCAATCAATAGATTGTGAGTTCAATTCCCTACACCCACGTGTTTGTACTTTAAAAAAATGAACAAAACACAACAACATTTCCAATAATGCTAATGGCTAAATATTTAACCAGCTTAAAAATAGGTTTAAATGAGTAGTTTTAACTTTAGTTTATTTTAGTTTGTGAACTTTCAAAATATCTATTTTGATGCACTTTTAAAATGTCCCTATCGTTTTCAAAAGTATTAACCTTTAATTTGTTTTCCTATGGTGTAGATTCTTAGCTTGAATAAGGATATTCAAAAGTATTGGCCAACTTATCGATGTGGGACAACATCAACCTGCCATAGGACCAAAGGCTCATTTTGGGCTCATGAGGTTTCAAAATTCTCTCTCTCTTCTTCTTTCTTTAGCTGAAAATCAACCAAAATTTGAGACTCATCCAAGTTTCTCTCTTGTTGTGTGATGCCCATTATTTGACATGTTATCATTGGTGCATGAAGTATGGTAAGTCGTATTATATTCTTCAATATTTCATAATATATATTTGTAATGTTTAATTTTTTGATCTTCATCTTTTATTTTGTTTCTACTTCTCTTTGATCTGTCTTTGCAGAAAAGCATGGGACTTGCGCAGCTCCTGTAATTGTGGGAGAATTTGATTACTTTTTGACAACCCTCACCATCTTCTCCAAGTACAATGTCACTGTAAGCTAATAGATCTTTGGTGCATATAACGTGTATAAAATTTTTCATTATTATGGAAGTTCGAAGAGGATCACGTGTTCAAAATAAAAATGACTTAAACGAGCATCCGGCTCAAGAAATCAGATCTAACCAAGATCGGTGAACCAGAACAATTTTGAGGGGCATGTTAAAGATCTAGGAGTTATAATAAACCTACGATGAACACCGACAAAGTTGATATTGAATTATGGTCTTAAAATTTGGTCTCTTTTTCCATTTTTTTGTTACCAGAAAGTTCTGAATGATGCTGGATTTGTAGCTTCCGATACTGAAAAGTATGCCCTTGGAGATGTGGTAGCTGCAGTTCAGGATGCTTTCAATGCAACTCCCAAGATTTCCTGCGCCAAAAAGGGAGCTGTTAAGGAACTTTGGCTATGCTTTGACAAAACTTTTCAGGTATATTTCATTTCTGTGTTTTCATCATAAAATCATACCCAAAAGCTTTATGATATGAATTTAATTGGCTAACTTTTCAAATTTAATTTCCTCGTTGAAGCCACGTGATTGCGATCTTGCGAATTCATGCCCGGATTTCATCAAGCTCCCAACATATGAGCCACAGGGTTAGTTTAATTATTTCTTTTAACCCAAAAGTTTAAGCTAAGTTATATGGTATAGGTTTAATCTTGTTTAATTTGTTTATTCTCTTTTAACCTTTATGTTTTGTGATATATAATTGCAGAGGCTAATAAGGTTGAGGGAGCAATTCCATGGATGGGTGATGCTGAAGCAATTATC

mRNA sequence

AAAAAAAAAATAAAACTATATACATCTTAATTAATGATACGAAGTTAAAGAACAATTCTACCCACCAAAAACAACTCGGATTACTTTGTATAAGTACGAGGATGCATGGAATTTGGAAAGCCAGATTTCAGTAGTACAAAAGCAGATTACTTTCCTATTTTGCGAAAAAAATGGCTTCTTCCTCCATCAAAGGCAACCCCACGGTGGTGGCCACCGTGTTCGTTCTTGTTGTTCTTCAGTTGCAGGCTGCGGCCTTTGCAGCGGGCGCCGCCCCCGGCAGCGAGGAGCAGCGGGAGTTCGACTACTTTGTGTTGGCCTTGCAATGGCCAGCCACTTCCTGCACAAATCCCGGCAAGTGTTGCCCCTCCAACGGTTGCTGTCGCGGAGCTGAGTCTCCCACTGAATTTACAATCCATGGGCTGTGGCCTCAGTATGAAGAGAAAGGATGGCCTTCATGTTGCAGTGATGCAAGCTTCAGTGAAAATGAGATTCTTAGCTTGAATAAGGATATTCAAAAGTATTGGCCAACTTATCGATGTGGGACAACATCAACCTGCCATAGGACCAAAGGCTCATTTTGGGCTCATGAGTATGAAAAGCATGGGACTTGCGCAGCTCCTGTAATTGTGGGAGAATTTGATTACTTTTTGACAACCCTCACCATCTTCTCCAAGTACAATGTCACTAAAGTTCTGAATGATGCTGGATTTGTAGCTTCCGATACTGAAAAGTATGCCCTTGGAGATGTGGTAGCTGCAGTTCAGGATGCTTTCAATGCAACTCCCAAGATTTCCTGCGCCAAAAAGGGAGCTGTTAAGGAACTTTGGCTATGCTTTGACAAAACTTTTCAGCCACGTGATTGCGATCTTGCGAATTCATGCCCGGATTTCATCAAGCTCCCAACATATGAGCCACAGGAGGCTAATAAGGTTGAGGGAGCAATTCCATGGATGGGTGATGCTGAAGCAATTATC

Coding sequence (CDS)

ATGGCTTCTTCCTCCATCAAAGGCAACCCCACGGTGGTGGCCACCGTGTTCGTTCTTGTTGTTCTTCAGTTGCAGGCTGCGGCCTTTGCAGCGGGCGCCGCCCCCGGCAGCGAGGAGCAGCGGGAGTTCGACTACTTTGTGTTGGCCTTGCAATGGCCAGCCACTTCCTGCACAAATCCCGGCAAGTGTTGCCCCTCCAACGGTTGCTGTCGCGGAGCTGAGTCTCCCACTGAATTTACAATCCATGGGCTGTGGCCTCAGTATGAAGAGAAAGGATGGCCTTCATGTTGCAGTGATGCAAGCTTCAGTGAAAATGAGATTCTTAGCTTGAATAAGGATATTCAAAAGTATTGGCCAACTTATCGATGTGGGACAACATCAACCTGCCATAGGACCAAAGGCTCATTTTGGGCTCATGAGTATGAAAAGCATGGGACTTGCGCAGCTCCTGTAATTGTGGGAGAATTTGATTACTTTTTGACAACCCTCACCATCTTCTCCAAGTACAATGTCACTAAAGTTCTGAATGATGCTGGATTTGTAGCTTCCGATACTGAAAAGTATGCCCTTGGAGATGTGGTAGCTGCAGTTCAGGATGCTTTCAATGCAACTCCCAAGATTTCCTGCGCCAAAAAGGGAGCTGTTAAGGAACTTTGGCTATGCTTTGACAAAACTTTTCAGCCACGTGATTGCGATCTTGCGAATTCATGCCCGGATTTCATCAAGCTCCCAACATATGAGCCACAGGAGGCTAATAAGGTTGAGGGAGCAATTCCATGGATGGGTGATGCTGAAGCAATTATC

Protein sequence

MASSSIKGNPTVVATVFVLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNPGKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWPTYRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGFVASDTEKYALGDVVAAVQDAFNATPKISCAKKGAVKELWLCFDKTFQPRDCDLANSCPDFIKLPTYEPQEANKVEGAIPWMGDAEAII

Homology

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BLAST of MC10g0280 vs. ExPASy Swiss-Prot
Match: P42814 (Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 6.8e-73
Identity = 122/248 (49.19%), Postives = 169/248 (68.15%), Query Frame = 0


Query: 18  VLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNPGKCCPSNGCCRGAESPT 77
           +L+++   A AFA      +  QREFDYF L+LQWP T C     CC  N CCRG+++PT
Sbjct: 7   LLLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPT 66

Query: 78  EFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWPTYRCGTTSTCHRTKGSFW 137
           +FTIHGLWP Y +  WPSCC  + F E EI +L   ++KYWP+  CG+ S+C+  KGSFW
Sbjct: 67  QFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFW 126

Query: 138 AHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGFVASDTEKYALGDVVAAV 197
            HE+EKHGTC++PV   E++YFLTTL ++ K+NVT VL  AG+VAS++EKY LG +V A+
Sbjct: 127 GHEWEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAI 186

Query: 198 QDAFNATPKISCAKKGAVKELWLCFDKTFQPRDC----DLAN--SCPDFIKLPTYEPQEA 257
           Q+AF+ TP++ C K+ A+ E+ +CF K F+PRDC    DL +  SCP ++ LP Y P + 
Sbjct: 187 QNAFHITPEVVC-KRDAIDEIRICFYKDFKPRDCVGSQDLTSRKSCPKYVSLPEYTPLDG 246

Query: 258 NKVEGAIP 260
             +   +P
Sbjct: 247 EAMVLKMP 253

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BLAST of MC10g0280 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.5e-27
Identity = 69/214 (32.24%), Postives = 104/214 (48.60%), Query Frame = 0


Query: 16  VFVLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNPGKCC-PSNGCCRGAE 75
           +F+L + QL   +FA          ++FD+F   LQWP   C +   CC P  G     +
Sbjct: 5   IFILALQQLYVQSFA----------QDFDFFYFVLQWPGAYCDSRHSCCYPQTG-----K 64

Query: 76  SPTEFTIHGLWPQYEEKGWPSCCS-DASFSENEILSLNKDIQKYWPTYRCGTTSTCHRTK 135
              +F IHGLWP Y+  GWP  C+ D+ F +  +  L  D+Q+ WPT  C +        
Sbjct: 65  PAADFGIHGLWPNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGM---- 124

Query: 136 GSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGFVASDTEKYALGDV 195
             FW HE+EKHGTCA   +  + DYF   L +  K N+   L +AG +  D + Y + D+
Sbjct: 125 -KFWTHEWEKHGTCAESEL-DQHDYFEAGLKLKQKANLLHALTNAG-IKPDDKFYEMKDI 184

Query: 196 VAAVQDAFNATPKISCAKKGA----VKELWLCFD 224
              ++      P I C K  +    + +++LC D
Sbjct: 185 ENTIKQVVGFAPGIECNKDSSHNSQLYQIYLCVD 196

[Close]

BLAST of MC10g0280 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.1e-26
Identity = 70/220 (31.82%), Postives = 117/220 (53.18%), Query Frame = 0


Query: 12  VVATVFVLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNPGKCCPSNGCCR 71
           ++A++ ++ +L +  + F+A     S    +FD+F    QWP + C    KCC  N    
Sbjct: 4   LLASLCLISLLVILPSVFSA-----SSSSEDFDFFYFVQQWPGSYCDTQKKCCYPN---- 63

Query: 72  GAESPTEFTIHGLWPQYEEKGWPSCCSDAS--FSENEILSLNKDIQKYWPTYRCGTTSTC 131
             +   +F IHGLWP Y++  +PS C DAS  F  + I  L   ++K WPT  C + S  
Sbjct: 64  SGKPAADFGIHGLWPNYKDGTYPSNC-DASKPFDSSTISDLLTSMKKSWPTLACPSGS-- 123

Query: 132 HRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGFVASDTEKYA 191
                +FW HE+EKHGTC+  VI  + +YF T L +  K N+   L  AG +  D + Y+
Sbjct: 124 ---GEAFWEHEWEKHGTCSESVI-DQHEYFQTALNLKQKTNLLGALTKAG-INPDGKSYS 183

Query: 192 LGDVVAAVQDAFNATPKISCAKKGA----VKELWLCFDKT 226
           L  +  +++++   TP + C + G+    + +++LC D++
Sbjct: 184 LESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDRS 206

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BLAST of MC10g0280 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 118.6 bits (296), Expect = 1.1e-25
Identity = 65/220 (29.55%), Postives = 109/220 (49.55%), Query Frame = 0


Query: 41  REFDYFVLALQWPATSCTNPGKCC-PSNGCCRGAESPTEFTIHGLWPQYEEKGWP-SCCS 100
           ++FD+F    QWPA+ C     CC P+ G     +   +F+IHGLWP Y++  WP +C  
Sbjct: 26  QDFDFFYFVQQWPASYCDTRRSCCYPTTG-----KPDEDFSIHGLWPNYKDGKWPQNCDR 85

Query: 101 DASFSENEILSLNKDIQKYWPTYRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDY 160
           ++S  E+E   L   ++K WP+  C ++         FW+HE+ KHGTC+A   + +  Y
Sbjct: 86  ESSLDESEFSDLISTMEKNWPSLACPSSDGL-----KFWSHEWLKHGTCSA---LNQHAY 145

Query: 161 FLTTLTIFSKYNVTKVLNDAGFVASDTEKYALGDVVAAVQDAFNATPKISC----AKKGA 220
           F T L   +K N+ + LN+AG    + + Y +  +  A++     TP I C         
Sbjct: 146 FQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEKGVGHTPFIECNVDSQGNHQ 205

Query: 221 VKELWLCFDKTFQP-RDCDL---ANSCPDFIKLPTYEPQE 251
           + +++LC D +     DC +      C   I+ P++   +
Sbjct: 206 LYQVYLCVDSSASKFIDCPIFPHGGKCGSKIEFPSFSTND 232

[Close]

BLAST of MC10g0280 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 115.5 bits (288), Expect = 9.0e-25
Identity = 63/215 (29.30%), Postives = 108/215 (50.23%), Query Frame = 0


Query: 41  REFDYFVLALQWPATSCTNPGKCC-PSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCS- 100
           ++FD+F    QWP + C     CC P+ G     +   +F IHGLWP   +  +PS C  
Sbjct: 27  KDFDFFYFVQQWPGSYCDTKQSCCYPTTG-----KPAADFGIHGLWPNNNDGTYPSNCDP 86

Query: 101 DASFSENEILSLNKDIQKYWPTYRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDY 160
           ++ + +++I  L   +Q+ WPT  C + S       +FW+HE+EKHGTCA  V+  +  Y
Sbjct: 87  NSPYDQSQISDLISSMQQNWPTLACPSGS-----GSTFWSHEWEKHGTCAESVLTNQHAY 146

Query: 161 FLTTLTIFSKYNVTKVLNDAGFVASDTEKYALGDVVAAVQDAFNATPKISC----AKKGA 220
           F   L + ++ ++  +L  A  +  D E Y L ++  A++ A   TP I C    +    
Sbjct: 147 FKKALDLKNQIDLLSILQGAD-IHPDGESYDLVNIRNAIKSAIGYTPWIQCNVDQSGNSQ 206

Query: 221 VKELWLCFDKTFQPR-DCDL--ANSCPDFIKLPTY 247
           + ++++C D +     +C +     C   I+ PT+
Sbjct: 207 LYQVYICVDGSGSSLIECPIFPGGKCGTSIEFPTF 230

[Close]

BLAST of MC10g0280 vs. NCBI nr
Match: XP_022140985.1 (ribonuclease 2-like [Momordica charantia])

HSP 1 Score: 561 bits (1447), Expect = 3.17e-202
Identity = 265/268 (98.88%), Postives = 266/268 (99.25%), Query Frame = 0


Query: 1   MASSSIKGNPTVVATVFVLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNP 60
           MASSSIKGNPT  ATVF+LVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNP
Sbjct: 1   MASSSIKGNPTAAATVFLLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNP 60

Query: 61  GKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWPT 120
           GKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWPT
Sbjct: 61  GKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWPT 120

Query: 121 YRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGF 180
           YRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGF
Sbjct: 121 YRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGF 180

Query: 181 VASDTEKYALGDVVAAVQDAFNATPKISCAKKGAVKELWLCFDKTFQPRDCDLANSCPDF 240
           VASDTEKYALGDVVAAVQDAFNATPKISCAKKGAVKELWLCFDKTFQPRDCDLANSCPDF
Sbjct: 181 VASDTEKYALGDVVAAVQDAFNATPKISCAKKGAVKELWLCFDKTFQPRDCDLANSCPDF 240

Query: 241 IKLPTYEPQEANKVEGAIPWMGDAEAII 268
           IKLPTYEPQEANKVEGAIPWMGDAEAII
Sbjct: 241 IKLPTYEPQEANKVEGAIPWMGDAEAII 268

[Close]

BLAST of MC10g0280 vs. NCBI nr
Match: XP_038876700.1 (ribonuclease 2-like [Benincasa hispida])

HSP 1 Score: 463 bits (1192), Expect = 2.27e-163
Identity = 214/269 (79.55%), Postives = 236/269 (87.73%), Query Frame = 0


Query: 1   MASSSIKGNPTVVATVFVLVVLQLQAAAFAAGA-APGSEEQREFDYFVLALQWPATSCTN 60
           MAS SIK  P     VF+L  LQL A AF   A AP   EQREFDYFVLALQWPATSC N
Sbjct: 1   MASFSIKAKPMAAPVVFLL--LQLLAVAFGFTAVAPSGSEQREFDYFVLALQWPATSCKN 60

Query: 61  PGKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWP 120
           PGKCCP+NGCCRG +SPTEFTIHGLWPQY ++GWPSCC+DA+F+ENEI  L +DIQKYWP
Sbjct: 61  PGKCCPANGCCRGVDSPTEFTIHGLWPQYNDRGWPSCCTDANFNENEINILAEDIQKYWP 120

Query: 121 TYRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAG 180
           TYRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGE+DYFLTTLTIFSKYNVTKVLNDAG
Sbjct: 121 TYRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTLTIFSKYNVTKVLNDAG 180

Query: 181 FVASDTEKYALGDVVAAVQDAFNATPKISCAKKGAVKELWLCFDKTFQPRDCDLANSCPD 240
           FVAS+TEKY + DVVAA+++ FN+TPKI+CAKKG VKELW+CFDK+F+PRDCDL NSCPD
Sbjct: 181 FVASNTEKYPIEDVVAAIKNEFNSTPKITCAKKGVVKELWICFDKSFEPRDCDLQNSCPD 240

Query: 241 FIKLPTYEPQEANKVEGAIPWMGDAEAII 268
           FIKLPTY+PQE NKVEGAIPW+ DAE II
Sbjct: 241 FIKLPTYDPQEPNKVEGAIPWIADAEEII 267

[Close]

BLAST of MC10g0280 vs. NCBI nr
Match: XP_004137162.1 (ribonuclease 2 [Cucumis sativus])

HSP 1 Score: 454 bits (1168), Expect = 1.03e-159
Identity = 217/271 (80.07%), Postives = 235/271 (86.72%), Query Frame = 0


Query: 1   MASSSIKGNPTVVATVFVLVVL-QLQAAAFAAGA-APGSEEQREFDYFVLALQWPATSCT 60
           MAS SIK      A VFV+VVL QL    F   A AP S EQREFDYFVLALQWP +SC 
Sbjct: 1   MASLSIKAK----AMVFVVVVLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCK 60

Query: 61  NPGKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYW 120
           NPGKCCPSN CCRGAESPTEFTIHGLWPQY EKGWPSCC+DASF+ENEI  L +DIQKYW
Sbjct: 61  NPGKCCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYW 120

Query: 121 PTYRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDA 180
           PTYRCGTTSTCH+TKGSFWAHEYEKHGTCAAPVIVGE+DYFLTT+TIFSKYNVTKVL+DA
Sbjct: 121 PTYRCGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDA 180

Query: 181 GFVASDTEKYALGDVVAAVQDAFN-ATPKISCAKKGAVKELWLCFDKTFQPRDCDLANSC 240
           GFVAS+TEKY + DVVAA+++ FN ATPKISCAKKGAVKELWLCFDK+F+PRDC+L NSC
Sbjct: 181 GFVASNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSC 240

Query: 241 PDFIKLPTYEPQEANKVEGAIPWMGDAEAII 268
           PDFIK PTYEPQE NKVEGAIPW+ D E II
Sbjct: 241 PDFIKFPTYEPQEPNKVEGAIPWIADVEEII 267

[Close]

BLAST of MC10g0280 vs. NCBI nr
Match: XP_022922895.1 (ribonuclease 2-like [Cucurbita moschata])

HSP 1 Score: 451 bits (1161), Expect = 1.12e-158
Identity = 213/268 (79.48%), Postives = 231/268 (86.19%), Query Frame = 0


Query: 1   MASSSIKGNPTVVATVFVLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNP 60
           MAS SIK NP VVA    L+VLQL A   AA   P   EQREFDYFVLALQWPATSC N 
Sbjct: 1   MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAET-PSGGEQREFDYFVLALQWPATSCKNT 60

Query: 61  GKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWPT 120
           GKCCP+NGCCRGA+SPTEFTIHGLWPQY EKGWPSCC+DA FS+NEI  L +DI+KYWPT
Sbjct: 61  GKCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQNEITLLKEDIEKYWPT 120

Query: 121 YRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGF 180
           YRCGTTS CH TKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTK L+DAGF
Sbjct: 121 YRCGTTSNCHGTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKALSDAGF 180

Query: 181 VASDTEKYALGDVVAAVQDAFNATPKISCAKKGAVKELWLCFDKTFQPRDCDLANSCPDF 240
           VAS+TEKY L DVV AV++A N+TPKISCAKKGAVKELW+CFDK F+PRDCDLANSCP+F
Sbjct: 181 VASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDCDLANSCPEF 240

Query: 241 IKLPTYEPQEANKVEGAIPWMGDAEAII 268
           IKLPTYEPQ+   VEGAIPW+ DAE +I
Sbjct: 241 IKLPTYEPQDV--VEGAIPWIADAEELI 265

[Close]

BLAST of MC10g0280 vs. NCBI nr
Match: XP_022984766.1 (ribonuclease 2-like [Cucurbita maxima])

HSP 1 Score: 447 bits (1150), Expect = 5.69e-157
Identity = 210/269 (78.07%), Postives = 232/269 (86.25%), Query Frame = 0


Query: 1   MASSSIKGNPTVVA-TVFVLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTN 60
           MAS  IK NP VVA  VF+L+ LQL A   AA    G   Q+EFDYFVLALQWPATSC N
Sbjct: 1   MASFYIKSNPMVVAPAVFLLLALQLVAVVLAAETPSGGGGQKEFDYFVLALQWPATSCKN 60

Query: 61  PGKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWP 120
            GKCCP+NGCCRGA+SPTEFTIHGLWPQY EKGWPSCC+DA F++NEI  L++DI+KYWP
Sbjct: 61  TGKCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLSEDIEKYWP 120

Query: 121 TYRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAG 180
           TYRCGTTS CH TKGSFWAHEYEKHGTCAAPVIVGE+DYFLTTLTIFSKYNVTK L+DAG
Sbjct: 121 TYRCGTTSNCHGTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTLTIFSKYNVTKALSDAG 180

Query: 181 FVASDTEKYALGDVVAAVQDAFNATPKISCAKKGAVKELWLCFDKTFQPRDCDLANSCPD 240
           FVAS+TEKY LGDVV AV++A N+TPKISCAKKGAVKELW+CFDK F+PRDCDLANSCP+
Sbjct: 181 FVASNTEKYPLGDVVDAVKNATNSTPKISCAKKGAVKELWICFDKNFEPRDCDLANSCPE 240

Query: 241 FIKLPTYEPQEANKVEGAIPWMGDAEAII 268
           FIKLPTYEPQ    VEGAIPW+ DAE +I
Sbjct: 241 FIKLPTYEPQGV--VEGAIPWIADAEELI 267

[Close]

BLAST of MC10g0280 vs. ExPASy TrEMBL
Match: A0A6J1CGP7 (Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111011504 PE=3 SV=1)

HSP 1 Score: 561 bits (1447), Expect = 1.53e-202
Identity = 265/268 (98.88%), Postives = 266/268 (99.25%), Query Frame = 0


Query: 1   MASSSIKGNPTVVATVFVLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNP 60
           MASSSIKGNPT  ATVF+LVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNP
Sbjct: 1   MASSSIKGNPTAAATVFLLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNP 60

Query: 61  GKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWPT 120
           GKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWPT
Sbjct: 61  GKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWPT 120

Query: 121 YRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGF 180
           YRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGF
Sbjct: 121 YRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGF 180

Query: 181 VASDTEKYALGDVVAAVQDAFNATPKISCAKKGAVKELWLCFDKTFQPRDCDLANSCPDF 240
           VASDTEKYALGDVVAAVQDAFNATPKISCAKKGAVKELWLCFDKTFQPRDCDLANSCPDF
Sbjct: 181 VASDTEKYALGDVVAAVQDAFNATPKISCAKKGAVKELWLCFDKTFQPRDCDLANSCPDF 240

Query: 241 IKLPTYEPQEANKVEGAIPWMGDAEAII 268
           IKLPTYEPQEANKVEGAIPWMGDAEAII
Sbjct: 241 IKLPTYEPQEANKVEGAIPWMGDAEAII 268

[Close]

BLAST of MC10g0280 vs. ExPASy TrEMBL
Match: A0A0A0L0P9 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_4G109540 PE=3 SV=1)

HSP 1 Score: 454 bits (1168), Expect = 4.99e-160
Identity = 217/271 (80.07%), Postives = 235/271 (86.72%), Query Frame = 0


Query: 1   MASSSIKGNPTVVATVFVLVVL-QLQAAAFAAGA-APGSEEQREFDYFVLALQWPATSCT 60
           MAS SIK      A VFV+VVL QL    F   A AP S EQREFDYFVLALQWP +SC 
Sbjct: 1   MASLSIKAK----AMVFVVVVLLQLLTVVFGFSAEAPSSSEQREFDYFVLALQWPGSSCK 60

Query: 61  NPGKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYW 120
           NPGKCCPSN CCRGAESPTEFTIHGLWPQY EKGWPSCC+DASF+ENEI  L +DIQKYW
Sbjct: 61  NPGKCCPSNACCRGAESPTEFTIHGLWPQYNEKGWPSCCTDASFNENEINILTEDIQKYW 120

Query: 121 PTYRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDA 180
           PTYRCGTTSTCH+TKGSFWAHEYEKHGTCAAPVIVGE+DYFLTT+TIFSKYNVTKVL+DA
Sbjct: 121 PTYRCGTTSTCHQTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTITIFSKYNVTKVLSDA 180

Query: 181 GFVASDTEKYALGDVVAAVQDAFN-ATPKISCAKKGAVKELWLCFDKTFQPRDCDLANSC 240
           GFVAS+TEKY + DVVAA+++ FN ATPKISCAKKGAVKELWLCFDK+F+PRDC+L NSC
Sbjct: 181 GFVASNTEKYPIEDVVAAIKNEFNNATPKISCAKKGAVKELWLCFDKSFEPRDCNLPNSC 240

Query: 241 PDFIKLPTYEPQEANKVEGAIPWMGDAEAII 268
           PDFIK PTYEPQE NKVEGAIPW+ D E II
Sbjct: 241 PDFIKFPTYEPQEPNKVEGAIPWIADVEEII 267

[Close]

BLAST of MC10g0280 vs. ExPASy TrEMBL
Match: A0A6J1EA39 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111430737 PE=3 SV=1)

HSP 1 Score: 451 bits (1161), Expect = 5.40e-159
Identity = 213/268 (79.48%), Postives = 231/268 (86.19%), Query Frame = 0


Query: 1   MASSSIKGNPTVVATVFVLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNP 60
           MAS SIK NP VVA    L+VLQL A   AA   P   EQREFDYFVLALQWPATSC N 
Sbjct: 1   MASFSIKSNPMVVAPAVFLLVLQLVAVVLAAET-PSGGEQREFDYFVLALQWPATSCKNT 60

Query: 61  GKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWPT 120
           GKCCP+NGCCRGA+SPTEFTIHGLWPQY EKGWPSCC+DA FS+NEI  L +DI+KYWPT
Sbjct: 61  GKCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFSQNEITLLKEDIEKYWPT 120

Query: 121 YRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGF 180
           YRCGTTS CH TKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTK L+DAGF
Sbjct: 121 YRCGTTSNCHGTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKALSDAGF 180

Query: 181 VASDTEKYALGDVVAAVQDAFNATPKISCAKKGAVKELWLCFDKTFQPRDCDLANSCPDF 240
           VAS+TEKY L DVV AV++A N+TPKISCAKKGAVKELW+CFDK F+PRDCDLANSCP+F
Sbjct: 181 VASNTEKYPLEDVVDAVKNATNSTPKISCAKKGAVKELWICFDKKFEPRDCDLANSCPEF 240

Query: 241 IKLPTYEPQEANKVEGAIPWMGDAEAII 268
           IKLPTYEPQ+   VEGAIPW+ DAE +I
Sbjct: 241 IKLPTYEPQDV--VEGAIPWIADAEELI 265

[Close]

BLAST of MC10g0280 vs. ExPASy TrEMBL
Match: A0A6J1J670 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111482949 PE=3 SV=1)

HSP 1 Score: 447 bits (1150), Expect = 2.75e-157
Identity = 210/269 (78.07%), Postives = 232/269 (86.25%), Query Frame = 0


Query: 1   MASSSIKGNPTVVA-TVFVLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTN 60
           MAS  IK NP VVA  VF+L+ LQL A   AA    G   Q+EFDYFVLALQWPATSC N
Sbjct: 1   MASFYIKSNPMVVAPAVFLLLALQLVAVVLAAETPSGGGGQKEFDYFVLALQWPATSCKN 60

Query: 61  PGKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWP 120
            GKCCP+NGCCRGA+SPTEFTIHGLWPQY EKGWPSCC+DA F++NEI  L++DI+KYWP
Sbjct: 61  TGKCCPANGCCRGADSPTEFTIHGLWPQYNEKGWPSCCTDAVFNQNEISLLSEDIEKYWP 120

Query: 121 TYRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAG 180
           TYRCGTTS CH TKGSFWAHEYEKHGTCAAPVIVGE+DYFLTTLTIFSKYNVTK L+DAG
Sbjct: 121 TYRCGTTSNCHGTKGSFWAHEYEKHGTCAAPVIVGEYDYFLTTLTIFSKYNVTKALSDAG 180

Query: 181 FVASDTEKYALGDVVAAVQDAFNATPKISCAKKGAVKELWLCFDKTFQPRDCDLANSCPD 240
           FVAS+TEKY LGDVV AV++A N+TPKISCAKKGAVKELW+CFDK F+PRDCDLANSCP+
Sbjct: 181 FVASNTEKYPLGDVVDAVKNATNSTPKISCAKKGAVKELWICFDKNFEPRDCDLANSCPE 240

Query: 241 FIKLPTYEPQEANKVEGAIPWMGDAEAII 268
           FIKLPTYEPQ    VEGAIPW+ DAE +I
Sbjct: 241 FIKLPTYEPQGV--VEGAIPWIADAEELI 267

[Close]

BLAST of MC10g0280 vs. ExPASy TrEMBL
Match: A0A1S3CHX1 (Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103501148 PE=3 SV=1)

HSP 1 Score: 445 bits (1144), Expect = 2.18e-156
Identity = 206/270 (76.30%), Postives = 232/270 (85.93%), Query Frame = 0


Query: 1   MASSSIKGNPTVVATVFVLVVLQLQAAAFAAGA-APGSEEQREFDYFVLALQWPATSCTN 60
           MAS SIKG P V    F+ ++++L    F   A AP   EQREFDYFVLALQWPATSC N
Sbjct: 1   MASFSIKGKPMV----FLFLIVELLTVVFGFSAEAPSGSEQREFDYFVLALQWPATSCKN 60

Query: 61  PGKCCPSNGCCRGAESPTEFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWP 120
            GKCCPSN CCRGA+SPTEFTIHGLWPQY EKGWPSCC+DASF+ENEI  L +DIQKYWP
Sbjct: 61  AGKCCPSNACCRGADSPTEFTIHGLWPQYNEKGWPSCCTDASFNENEIGILAEDIQKYWP 120

Query: 121 TYRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAG 180
           TYRCGT+STCHRTKGSFWAHEYEKHGTCAAPV+VGE+DYFLT LTIFSKYNVTKVL++ G
Sbjct: 121 TYRCGTSSTCHRTKGSFWAHEYEKHGTCAAPVLVGEYDYFLTALTIFSKYNVTKVLSEGG 180

Query: 181 FVASDTEKYALGDVVAAVQDAF-NATPKISCAKKGAVKELWLCFDKTFQPRDCDLANSCP 240
           FVAS+TEKY + DV+AA+++ F NATPKI+CAKKG VKELWLCFDK+F+PRDC+L +SCP
Sbjct: 181 FVASNTEKYPIEDVIAAIKNKFYNATPKITCAKKGVVKELWLCFDKSFEPRDCNLQHSCP 240

Query: 241 DFIKLPTYEPQEANKVEGAIPWMGDAEAII 268
           DFIKLPTYEPQE NKVEGAIPW+ D E II
Sbjct: 241 DFIKLPTYEPQEPNKVEGAIPWIADVEEII 266

[Close]

BLAST of MC10g0280 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 275.4 bits (703), Expect = 4.8e-74
Identity = 122/248 (49.19%), Postives = 169/248 (68.15%), Query Frame = 0


Query: 18  VLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNPGKCCPSNGCCRGAESPT 77
           +L+++   A AFA      +  QREFDYF L+LQWP T C     CC  N CCRG+++PT
Sbjct: 7   LLLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPT 66

Query: 78  EFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWPTYRCGTTSTCHRTKGSFW 137
           +FTIHGLWP Y +  WPSCC  + F E EI +L   ++KYWP+  CG+ S+C+  KGSFW
Sbjct: 67  QFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFW 126

Query: 138 AHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGFVASDTEKYALGDVVAAV 197
            HE+EKHGTC++PV   E++YFLTTL ++ K+NVT VL  AG+VAS++EKY LG +V A+
Sbjct: 127 GHEWEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAI 186

Query: 198 QDAFNATPKISCAKKGAVKELWLCFDKTFQPRDC----DLAN--SCPDFIKLPTYEPQEA 257
           Q+AF+ TP++ C K+ A+ E+ +CF K F+PRDC    DL +  SCP ++ LP Y P + 
Sbjct: 187 QNAFHITPEVVC-KRDAIDEIRICFYKDFKPRDCVGSQDLTSRKSCPKYVSLPEYTPLDG 246

Query: 258 NKVEGAIP 260
             +   +P
Sbjct: 247 EAMVLKMP 253

[Close]

BLAST of MC10g0280 vs. TAIR 10
Match: AT2G39780.2 (ribonuclease 2 )

HSP 1 Score: 214.9 bits (546), Expect = 7.8e-56
Identity = 103/248 (41.53%), Postives = 142/248 (57.26%), Query Frame = 0


Query: 18  VLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNPGKCCPSNGCCRGAESPT 77
           +L+++   A AFA      +  QREFDYF L+LQWP T C     CC  N CCRG+++PT
Sbjct: 7   LLLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPT 66

Query: 78  EFTIHGLWPQYEEKGWPSCCSDASFSENEILSLNKDIQKYWPTYRCGTTSTCHRTKGSFW 137
           +FTIHGLWP Y +  WPSCC  + F E EI +L   ++KYWP+  CG+ S+C+  KGSFW
Sbjct: 67  QFTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFW 126

Query: 138 AHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGFVASDTEKYALGDVVAAV 197
            HE                                 VL  AG+VAS++EKY LG +V A+
Sbjct: 127 GHE--------------------------------DVLYQAGYVASNSEKYPLGGIVTAI 186

Query: 198 QDAFNATPKISCAKKGAVKELWLCFDKTFQPRDC----DLAN--SCPDFIKLPTYEPQEA 257
           Q+AF+ TP++ C K+ A+ E+ +CF K F+PRDC    DL +  SCP ++ LP Y P + 
Sbjct: 187 QNAFHITPEVVC-KRDAIDEIRICFYKDFKPRDCVGSQDLTSRKSCPKYVSLPEYTPLDG 221

Query: 258 NKVEGAIP 260
             +   +P
Sbjct: 247 EAMVLKMP 221

[Close]

BLAST of MC10g0280 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 127.1 bits (318), Expect = 2.1e-29
Identity = 70/219 (31.96%), Postives = 109/219 (49.77%), Query Frame = 0


Query: 37  SEEQREFDYFVLALQWPATSCTNPGKCC-PSNGCCRGAESPTEFTIHGLWPQYEEKGWPS 96
           S  Q +FD+F   LQWP   C     CC P++G     +   +F IHGLWP Y    WPS
Sbjct: 22  SRPQEDFDFFYFVLQWPGAYCDTSRACCYPTSG-----KPAADFGIHGLWPNYNGGSWPS 81

Query: 97  CCS-DASFSENEILSLNKDIQKYWPTYRCGTTSTCHRTKGSFWAHEYEKHGTCAAPVIVG 156
            C  D+ F  ++I  L   ++K WPT  C +         +FW HE+EKHGTC+  V + 
Sbjct: 82  NCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGF-----NFWEHEWEKHGTCSESV-MD 141

Query: 157 EFDYFLTTLTIFSKYNVTKVLNDAGFVASDTEKYALGDVVAAVQDAFNATPKISCAK--- 216
           + +YF   L +  K N+ ++L ++G +  D   Y L  +  A++D    TP I C K   
Sbjct: 142 QHEYFENALKLKQKANLLQILKNSG-INPDDGFYNLDKITNAIKDGIGFTPGIECNKDPE 201

Query: 217 -KGAVKELWLCFDKT-FQPRDCDL--ANSCPDFIKLPTY 247
               + ++++C D +  +  +C +    SCP  I+   +
Sbjct: 202 RNAQLHQIYICVDTSGTEFIECPVLPRGSCPSQIQFSKF 228

[Close]

BLAST of MC10g0280 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 124.8 bits (312), Expect = 1.1e-28
Identity = 69/214 (32.24%), Postives = 104/214 (48.60%), Query Frame = 0


Query: 16  VFVLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNPGKCC-PSNGCCRGAE 75
           +F+L + QL   +FA          ++FD+F   LQWP   C +   CC P  G     +
Sbjct: 5   IFILALQQLYVQSFA----------QDFDFFYFVLQWPGAYCDSRHSCCYPQTG-----K 64

Query: 76  SPTEFTIHGLWPQYEEKGWPSCCS-DASFSENEILSLNKDIQKYWPTYRCGTTSTCHRTK 135
              +F IHGLWP Y+  GWP  C+ D+ F +  +  L  D+Q+ WPT  C +        
Sbjct: 65  PAADFGIHGLWPNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGM---- 124

Query: 136 GSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGFVASDTEKYALGDV 195
             FW HE+EKHGTCA   +  + DYF   L +  K N+   L +AG +  D + Y + D+
Sbjct: 125 -KFWTHEWEKHGTCAESEL-DQHDYFEAGLKLKQKANLLHALTNAG-IKPDDKFYEMKDI 184

Query: 196 VAAVQDAFNATPKISCAKKGA----VKELWLCFD 224
              ++      P I C K  +    + +++LC D
Sbjct: 185 ENTIKQVVGFAPGIECNKDSSHNSQLYQIYLCVD 196

[Close]

BLAST of MC10g0280 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 120.9 bits (302), Expect = 1.5e-27
Identity = 70/220 (31.82%), Postives = 117/220 (53.18%), Query Frame = 0


Query: 12  VVATVFVLVVLQLQAAAFAAGAAPGSEEQREFDYFVLALQWPATSCTNPGKCCPSNGCCR 71
           ++A++ ++ +L +  + F+A     S    +FD+F    QWP + C    KCC  N    
Sbjct: 4   LLASLCLISLLVILPSVFSA-----SSSSEDFDFFYFVQQWPGSYCDTQKKCCYPN---- 63

Query: 72  GAESPTEFTIHGLWPQYEEKGWPSCCSDAS--FSENEILSLNKDIQKYWPTYRCGTTSTC 131
             +   +F IHGLWP Y++  +PS C DAS  F  + I  L   ++K WPT  C + S  
Sbjct: 64  SGKPAADFGIHGLWPNYKDGTYPSNC-DASKPFDSSTISDLLTSMKKSWPTLACPSGS-- 123

Query: 132 HRTKGSFWAHEYEKHGTCAAPVIVGEFDYFLTTLTIFSKYNVTKVLNDAGFVASDTEKYA 191
                +FW HE+EKHGTC+  VI  + +YF T L +  K N+   L  AG +  D + Y+
Sbjct: 124 ---GEAFWEHEWEKHGTCSESVI-DQHEYFQTALNLKQKTNLLGALTKAG-INPDGKSYS 183

Query: 192 LGDVVAAVQDAFNATPKISCAKKGA----VKELWLCFDKT 226
           L  +  +++++   TP + C + G+    + +++LC D++
Sbjct: 184 LESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDRS 206

The following BLAST results are available for this feature:

BLAST of MC10g0280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42814	6.8e-73	49.19	Ribonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=RNS2 PE=2 SV=1	[more]
P42815	1.5e-27	32.24	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
P42813	2.1e-26	31.82	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P80196	1.1e-25	29.55	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]
P80022	9.0e-25	29.30	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]

BLAST of MC10g0280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140985.1	3.17e-202	98.88	ribonuclease 2-like [Momordica charantia]	[more]
XP_038876700.1	2.27e-163	79.55	ribonuclease 2-like [Benincasa hispida]	[more]
XP_004137162.1	1.03e-159	80.07	ribonuclease 2 [Cucumis sativus]	[more]
XP_022922895.1	1.12e-158	79.48	ribonuclease 2-like [Cucurbita moschata]	[more]
XP_022984766.1	5.69e-157	78.07	ribonuclease 2-like [Cucurbita maxima]	[more]

BLAST of MC10g0280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CGP7	1.53e-202	98.88	Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111011504 PE=3 SV=1	[more]
A0A0A0L0P9	4.99e-160	80.07	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_4G109540 PE=3 SV=1	[more]
A0A6J1EA39	5.40e-159	79.48	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111430737 PE=3 SV=1	[more]
A0A6J1J670	2.75e-157	78.07	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111482949 PE=3 SV=1	[more]
A0A1S3CHX1	2.18e-156	76.30	Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103501148 PE=3 SV=1	[more]

BLAST of MC10g0280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39780.1	4.8e-74	49.19	ribonuclease 2	[more]
AT2G39780.2	7.8e-56	41.53	ribonuclease 2	[more]
AT1G14220.1	2.1e-29	31.96	Ribonuclease T2 family protein	[more]
AT1G26820.1	1.1e-28	32.24	ribonuclease 3	[more]
AT2G02990.1	1.5e-27	31.82	ribonuclease 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 39..248 e-value: 9.0E-72 score: 243.4
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 42..245
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 43..231 e-value: 5.0E-47 score: 160.4
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 22..253
None	No IPR available	PANTHER	PTHR11240:SF66	OS01G0897300 PROTEIN	coord: 22..253
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 79..86
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 136..147
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 43..239 e-value: 2.10252E-59 score: 185.228

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g0280.1	MC10g0280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016020	membrane
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding