MC10g0231 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g0231
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	gibberellin 20-oxidase-like protein
Location	MC10: 1795867 .. 1797820 (-)
RNA-Seq Expression	MC10g0231
Synteny	MC10g0231

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGGAATCTCAAACCTGTGTGGAGCTTCCAGTGATCGACCTTTCTCAGCCTTTGAGCGAATCGATTCTCTGCTCTCTTTGTGCGGCCTGCAACGAATGGGGATTCTTCTACATCACCAACCATGGCGTCTCCGACGAATTTTACCAGAAACTGATCTCCATCGCCGATGAACTGTTTCGTCTTCCTCAAGACACCAAAATCAAAGTCGGAATTTCGTCGTACGTGCCGAGATTTACGGCGTCGCCTTTCTTTGAGAGCTTCAAGCTTTCGGGGCCGGACTTCTCTGCCTCTGCTCAACTCTTGACGGAAGCGCTCCTCGGCCATCACAATACTCAATTTAGGTAAGAAATAGATTACCAATAAGTTTTTTCCCTTTTTAGGAAGAATAAGTTTGCGTTTTTTTTTCAATTCCAAAAAAAGTCTTAACAAAGTCGAATGATTAAATATTTCAATTTGTCACCTGTGATTTTGAGATTAATTAAAATATATATATATATATATGTTATATAAACTAAATGAAGTATACGATAATTGATATATACTTANTCGTGAGAGGTTTCATCAACCAGATTAAAATTAAATTCAAAACATCTCTTAAATTTTTAGGGGTCAATTTGAATTTTTTTTTGTACAACGCATAACCTCTTGATTGAAGATGTATGTCAATTAGTGATGCTCACTTTGATGCGGTCAAATTGAAATTAATGAGAGATTAAATTGGTAATTTATTTTATAAAGTTTTGTGCTTTTTGTTATCTGATTTCTTAATTGAAGAAAAATAAATTTTGTTTAGTAATGTTTTTAATCGAGTGTACGGTAATTATCAAATTATAATTTTATAATAAAAATTAAATGATTTTTTGTTAAAAGTATAATTTTATAACTACGGTAATTACAATAGATTACAGAGAAGAAAAAAGTTAAGGACAGGAAATAAAAGTTATAGAGATATATATCTCTTTATGATGGGGAGAATAATTTGGAGAACGTTAATATAACCATCTTTTTTTTTTAATATAACAATTTTAAAAATATTTTTTAATATTGACAACGGTAAAAAAAATTTATTTTAAAAAATATAATAGAAATTAATATATTTATCGAGCATACTTTATTCTGATTTGTTTAGGTCTCATAATGCATAGTATCAACTTGAACTCGCGGCCAACTTGAATGTAACTTAATTAAGAGGTGACTAAAAAAATCGAAGAATCTTCCACTTAAAAATAAATAAATCATCAAATAAACAATCGAATAATTTTTTTTTTCTTTTTTCCCCTTTCTACCAAGTTCATTTACATAATGTCCCTCTTCTTCGTTCATTTATTTATGAAAATAATCTCTCTTGTGAGTTGAATTTGTTTGCAGCAACTTACTAGAGGAATATGGAAGCAAAATGACTGACCTATCTAAAAGAATAGTCAAGCTATTACTAAAGGTCTTGGGAGATAATTTCGAAAACAAATTTTATGATTATGAGTTCAAAAACTGTGATGGATATCTTAGGATAAACAACTACTCTCCTCGAGACTCCAATGAAGAAGTTGAAGGGCTTGGGAAACACACTGATATTAGCTGCGTGACAATTCTCTTTCAAGATAAACTTGGTGGACTTCAGATGAGATCTAAAGGAGGAGAGTGGATGGACATAAGGCCTTGTGAGAACGCTCTTGTGGTGAACATTGGGGATTTTATGCAAGCTTGGAGCAATGAGAGGTTGAGATCAGCAGAACATCGAGTTGTTTTGAGGCAAAATGTAAAGAGATTCTCTATGGCATTCTTTTGGTCATATGTAGATGATGAGGAGATATATGCCCCAAGCGAGGTCGTTGGAGAAGGGAATGTGAGGCTTTATCGTACATTTTCAACAAAAGAGTATAGGATGTTCAGGGCAACTGGTGCGAGAGGGACCTATGAGAAAGTTGGAGCCAATGTAAAAGAATTTGCTGGA

mRNA sequence

ATGTCGGAATCTCAAACCTGTGTGGAGCTTCCAGTGATCGACCTTTCTCAGCCTTTGAGCGAATCGATTCTCTGCTCTCTTTGTGCGGCCTGCAACGAATGGGGATTCTTCTACATCACCAACCATGGCGTCTCCGACGAATTTTACCAGAAACTGATCTCCATCGCCGATGAACTGTTTCGTCTTCCTCAAGACACCAAAATCAAAGTCGGAATTTCGTCGTACGTGCCGAGATTTACGGCGTCGCCTTTCTTTGAGAGCTTCAAGCTTTCGGGGCCGGACTTCTCTGCCTCTGCTCAACTCTTGACGGAAGCGCTCCTCGGCCATCACAATACTCAATTTAGCAACTTACTAGAGGAATATGGAAGCAAAATGACTGACCTATCTAAAAGAATAGTCAAGCTATTACTAAAGGTCTTGGGAGATAATTTCGAAAACAAATTTTATGATTATGAGTTCAAAAACTGTGATGGATATCTTAGGATAAACAACTACTCTCCTCGAGACTCCAATGAAGAAGTTGAAGGGCTTGGGAAACACACTGATATTAGCTGCGTGACAATTCTCTTTCAAGATAAACTTGGTGGACTTCAGATGAGATCTAAAGGAGGAGAGTGGATGGACATAAGGCCTTGTGAGAACGCTCTTGTGGTGAACATTGGGGATTTTATGCAAGCTTGGAGCAATGAGAGGTTGAGATCAGCAGAACATCGAGTTGTTTTGAGGCAAAATGTAAAGAGATTCTCTATGGCATTCTTTTGGTCATATGTAGATGATGAGGAGATATATGCCCCAAGCGAGGTCGTTGGAGAAGGGAATGTGAGGCTTTATCGTACATTTTCAACAAAAGAGTATAGGATGTTCAGGGCAACTGGTGCGAGAGGGACCTATGAGAAAGTTGGAGCCAATGTAAAAGAATTTGCTGGA

Coding sequence (CDS)

ATGTCGGAATCTCAAACCTGTGTGGAGCTTCCAGTGATCGACCTTTCTCAGCCTTTGAGCGAATCGATTCTCTGCTCTCTTTGTGCGGCCTGCAACGAATGGGGATTCTTCTACATCACCAACCATGGCGTCTCCGACGAATTTTACCAGAAACTGATCTCCATCGCCGATGAACTGTTTCGTCTTCCTCAAGACACCAAAATCAAAGTCGGAATTTCGTCGTACGTGCCGAGATTTACGGCGTCGCCTTTCTTTGAGAGCTTCAAGCTTTCGGGGCCGGACTTCTCTGCCTCTGCTCAACTCTTGACGGAAGCGCTCCTCGGCCATCACAATACTCAATTTAGCAACTTACTAGAGGAATATGGAAGCAAAATGACTGACCTATCTAAAAGAATAGTCAAGCTATTACTAAAGGTCTTGGGAGATAATTTCGAAAACAAATTTTATGATTATGAGTTCAAAAACTGTGATGGATATCTTAGGATAAACAACTACTCTCCTCGAGACTCCAATGAAGAAGTTGAAGGGCTTGGGAAACACACTGATATTAGCTGCGTGACAATTCTCTTTCAAGATAAACTTGGTGGACTTCAGATGAGATCTAAAGGAGGAGAGTGGATGGACATAAGGCCTTGTGAGAACGCTCTTGTGGTGAACATTGGGGATTTTATGCAAGCTTGGAGCAATGAGAGGTTGAGATCAGCAGAACATCGAGTTGTTTTGAGGCAAAATGTAAAGAGATTCTCTATGGCATTCTTTTGGTCATATGTAGATGATGAGGAGATATATGCCCCAAGCGAGGTCGTTGGAGAAGGGAATGTGAGGCTTTATCGTACATTTTCAACAAAAGAGTATAGGATGTTCAGGGCAACTGGTGCGAGAGGGACCTATGAGAAAGTTGGAGCCAATGTAAAAGAATTTGCTGGA

Protein sequence

MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELFRLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKVGANVKEFAG

Homology

[Close]

BLAST of MC10g0231 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 2.6e-84
Identity = 158/318 (49.69%), Postives = 221/318 (69.50%), Query Frame = 0


Query: 8   VELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELFRLPQDTK 67
           +ELPV D+S+PLSES L SL  AC EWGFFY+TNHGVS + Y+KL   +  +F L  + K
Sbjct: 3   LELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEEK 62

Query: 68  IKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGH-HNTQFSNLLEEYGSKMT 127
           +K+G S+Y PRF ASPFFES ++SGPDF ASA+   +A      + +FS L++EYG KMT
Sbjct: 63  MKMGASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEYGEKMT 122

Query: 128 DLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYS-PRDSNEE---------VEG 187
            L ++I+K +L   GD+  +K+Y+ EF NC GY RINNY+ P D  ++         +EG
Sbjct: 123 KLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQDLIEG 182

Query: 188 LGKHTDISCVTILFQDKLGGLQMRSKGG-EWMDIRPCENALVVNIGDFMQAWSNERLRSA 247
           LG HTD+SC+TI+ QD +GGLQ+R++ G   MDI P + ALVVN+GD + AW+N RLRS+
Sbjct: 183 LGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLLHAWTNGRLRSS 242

Query: 248 EHRVVLRQN---VKRFSMAFFWSYVDDEEIYAPSEVVG--EGNVRLYRTFSTKEYRMFRA 307
           +HRV+L++      RFS+AFFW + D + ++AP EVVG  EG +R++R+F   +Y  FR 
Sbjct: 243 QHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEG-MRVFRSFKCGDYLRFRE 302

Query: 308 TGARGTYEKVGANVKEFA 309
           +  +G +EKVG  V++FA
Sbjct: 303 SNEKGKFEKVGDTVEDFA 319

[Close]

BLAST of MC10g0231 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 3.8e-35
Identity = 98/310 (31.61%), Postives = 159/310 (51.29%), Query Frame = 0


Query: 8   VELPVIDLSQPLS-ESILCSLC-----AACNEWGFFYITNHGVSDEFYQKLISIADELFR 67
           ++LPVIDLS   S E +    C     AA  EWGFF I NHG+  + ++ ++    +LF 
Sbjct: 38  IKLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFD 97

Query: 68  LPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQ---------LLTE-ALLGHHN 127
            P   K++   S             S++   P  ++ AQ         +L+E + +    
Sbjct: 98  QPFSVKVRERFSDLSK--------NSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDR 157

Query: 128 TQFSNLLEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSN 187
                ++E Y  ++  +++ I ++L K +  N  +++++  F+  + +LR+N Y P    
Sbjct: 158 NNLRTIVETYVQEIARVAQMICEILGKQV--NVSSEYFENIFELENSFLRLNKYHPSVFG 217

Query: 188 EEVEGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNER 247
            EV GL  HTD S +TIL QD++GGL++ +  G+W+ ++PC  AL VNIGD  QA SN  
Sbjct: 218 SEVFGLVPHTDTSFLTILSQDQIGGLELEN-NGQWISVKPCLEALTVNIGDMFQALSNGV 277

Query: 248 LRSAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRAT 302
            +S  HRV+   N++R S+AFF         Y  +E+   G  + YR FS +EY+     
Sbjct: 278 YQSVRHRVISPANIERMSIAFFVC------PYLETEIDCFGYPKKYRRFSFREYKEQSEH 330

[Close]

BLAST of MC10g0231 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 146.7 bits (369), Expect = 4.2e-34
Identity = 98/294 (33.33%), Postives = 147/294 (50.00%), Query Frame = 0


Query: 8   VELPVIDLSQPLS------ESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELFR 67
           VELPVID+S+ +       E    ++  A  EWGFF + NHG+S +  +K+      +FR
Sbjct: 40  VELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFR 99

Query: 68  LPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQL---------LTEALLGHHNT 127
            P D K K        +F+A     S++   P  ++  QL         +T+       T
Sbjct: 100 EPFDKKSK------SEKFSAG----SYRWGTPSATSIRQLSWSEAFHVPMTDISDNKDFT 159

Query: 128 QFSNLLEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNE 187
             S+ +E++ S+   L+  + ++L +  G N  + F+         YLR+N Y P     
Sbjct: 160 TLSSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPPCPKPS 219

Query: 188 EVEGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERL 247
           EV GL  HTD   +TIL+QD++GGLQ+  K   W+ ++P   AL++NIGD  QAWSN   
Sbjct: 220 EVYGLMPHTDSDFLTILYQDQVGGLQL-IKDNRWIAVKPNPKALIINIGDLFQAWSNGMY 279

Query: 248 RSAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYR 287
           +S EHRV+    V+RFS A+F     D  I   S+         YR FS +E+R
Sbjct: 280 KSVEHRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPA------YRNFSFREFR 314

[Close]

BLAST of MC10g0231 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 7.9e-33
Identity = 92/291 (31.62%), Postives = 143/291 (49.14%), Query Frame = 0


Query: 10  LPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELFRLPQDTKIK 69
           +PVIDLS P    +   +  A   WG F I NHG+S +    + S++  LF +P + K++
Sbjct: 49  IPVIDLSNP---DVTTLIGDASKTWGAFQIANHGISQKLLDDIESLSKTLFDMPSERKLE 108

Query: 70  V-----GISSY-VPRFTASPFFESFKLSGPDFSA---SAQLLTEALLGHHNTQFSNLLEE 129
                 G+S Y  PR   SPFFE  K+    F+    S +     L  H +T++  +++E
Sbjct: 109 AASSDKGVSGYGEPRI--SPFFEK-KMWSEGFTIADDSYRNHFNTLWPHDHTKYCGIIQE 168

Query: 130 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCD-----GYLRINNYSPRDSNEEVE 189
           Y  +M  L+ R++  +L  LG   E+  + ++ +        G +R+N+Y      E   
Sbjct: 169 YVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHYPVCPEPERAM 228

Query: 190 GLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSA 249
           GL  HTD + +TIL Q   GGLQ+  +   W+ + P    LVVNIGD     SN ++ S 
Sbjct: 229 GLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPAPGVLVVNIGDLFHILSNGKIPSV 288

Query: 250 EHRVVLRQNVKRFSMAFFW-SYVDDEEIYAPSEVVGEGNVRLYRTFSTKEY 286
            HR  +     R S+A+ W     D +I   S++ G     LYR+ + KEY
Sbjct: 289 VHRAKVNHTRSRISIAYLWGGPAGDVQIAPISKLTGPAEPSLYRSITWKEY 333

[Close]

BLAST of MC10g0231 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 8.8e-32
Identity = 97/308 (31.49%), Postives = 149/308 (48.38%), Query Frame = 0


Query: 9   ELPVIDLSQPLSESILC--SLCAACNEWGFFYITNHGVSDEFYQKLISIADELFRLPQDT 68
           ELPVIDL+  +    +C   +  A +EWGFF +TNHGV     ++L      +FR P   
Sbjct: 41  ELPVIDLAGLMEAEQVCRADMVRAASEWGFFQVTNHGVPQALLRELHDAQVAVFRRPFQE 100

Query: 69  KIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHH----------NTQFSN 128
           K+   +  + P        ES++   P      QL       +H          + +   
Sbjct: 101 KVTERLLGFSP--------ESYRWGTPTAKCLEQLSWSE--AYHIPMTTPRPSTSIRARA 160

Query: 129 LLEEYGSKMTDLSKRIVKLLLKVL-GDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVE 188
           ++EE    M +L++++ ++L++ L G            + C  +LR+N Y P        
Sbjct: 161 VIEEVSRAMYELAQKLAEILMRGLPGAGEGETMVTTREETC--FLRLNRYPPCAMAMGGF 220

Query: 189 GLGKHTDISCVTILF--QDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLR 248
           GL  HTD   +TI+   QD +GGLQ+  KGG W+ ++P  + L+VN+GD +QAWSN+  +
Sbjct: 221 GLCPHTDSDLLTIVHQQQDTVGGLQL-LKGGRWVAVKPSPSTLIVNVGDLLQAWSNDVYK 280

Query: 249 SAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGA 302
           S EHRV+    ++RFSMAFF        I   S  V + +   YR+F+  EYR       
Sbjct: 281 SVEHRVMANATLERFSMAFFLCPSYHTLIIPSSSHVHDDDAH-YRSFTFGEYRKQIMEDV 334

[Close]

BLAST of MC10g0231 vs. NCBI nr
Match: XP_022141254.1 (gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia])

HSP 1 Score: 630 bits (1625), Expect = 6.26e-228
Identity = 308/309 (99.68%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF
Sbjct: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60

Query: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120
           RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE
Sbjct: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120

Query: 121 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGLGKH 180
           YGSKMT+LSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGLGKH
Sbjct: 121 YGSKMTBLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGLGKH 180

Query: 181 TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV 240
           TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV
Sbjct: 181 TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV 240

Query: 241 LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV 300
           LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV
Sbjct: 241 LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV 300

Query: 301 GANVKEFAG 309
           GANVKEFAG
Sbjct: 301 GANVKEFAG 309

[Close]

BLAST of MC10g0231 vs. NCBI nr
Match: XP_022141253.1 (gibberellin 20-oxidase-like protein [Momordica charantia])

HSP 1 Score: 524 bits (1349), Expect = 8.70e-186
Identity = 260/314 (82.80%), Postives = 276/314 (87.90%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           MSESQTCVELPVID+ + LSES L SL  ACNEWGFFYI NHGVS EFY +LISIADELF
Sbjct: 1   MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF 60

Query: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120
           RLPQ+TK KVGIS YVPRF ASPFFESFK +GPDFSASA  LTEAL  H NT+FSNLLEE
Sbjct: 61  RLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLEE 120

Query: 121 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSN-----EEVE 180
           YGSKM +LSKRIV+LLLK+L D FENKFYD EF NC G L INNYSPRDSN     EE+E
Sbjct: 121 YGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEIE 180

Query: 181 GLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSA 240
           GLGKHTDISCVTILFQD +GGLQMRSKGGEWMDI+PCE+ALVVNIGDFMQAWSNERLRSA
Sbjct: 181 GLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRSA 240

Query: 241 EHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARG 300
           EHRVVLRQNVKRFSM+FFWSYVDD EIYAPSEVVGEGNVRLYR FS KEY+MFRAT ARG
Sbjct: 241 EHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSARG 300

Query: 301 TYEKVGANVKEFAG 309
            YEKVGA VK+FAG
Sbjct: 301 NYEKVGATVKDFAG 314

[Close]

BLAST of MC10g0231 vs. NCBI nr
Match: XP_022141255.1 (gibberellin 20-oxidase-like protein isoform X2 [Momordica charantia])

HSP 1 Score: 516 bits (1329), Expect = 1.49e-183
Identity = 263/309 (85.11%), Postives = 263/309 (85.11%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF
Sbjct: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60

Query: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120
           RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQF      
Sbjct: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQF------ 120

Query: 121 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGLGKH 180
                                                   RINNYSPRDSNEEVEGLGKH
Sbjct: 121 ----------------------------------------RINNYSPRDSNEEVEGLGKH 180

Query: 181 TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV 240
           TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV
Sbjct: 181 TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV 240

Query: 241 LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV 300
           LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV
Sbjct: 241 LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV 263

Query: 301 GANVKEFAG 309
           GANVKEFAG
Sbjct: 301 GANVKEFAG 263

[Close]

BLAST of MC10g0231 vs. NCBI nr
Match: XP_022140867.1 (gibberellin 20-oxidase-like protein [Momordica charantia])

HSP 1 Score: 492 bits (1267), Expect = 3.09e-173
Identity = 248/318 (77.99%), Postives = 274/318 (86.16%), Query Frame = 0


Query: 1   MSESQT-CVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADEL 60
           MSES++  VELPVID+ + LSES L SL  AC+EWGFFYI NHGVS EFYQKLISIAD+L
Sbjct: 1   MSESKSYTVELPVIDVYEDLSESALSSLSEACSEWGFFYIRNHGVSKEFYQKLISIADQL 60

Query: 61  FRLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLE 120
           F LP +TK KVG S Y+PRF ASPF+ESFK SGPDFSASA  LTE LLG H+ +FSNLL+
Sbjct: 61  FCLPHETKNKVGFSCYIPRFIASPFYESFKFSGPDFSASAHHLTETLLGKHDAEFSNLLK 120

Query: 121 EYGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNE-----EV 180
           EYGSKMTD+S+RIVKLLLK+LGDNFENKFYD EF NCDGYLR+NNYSP+DSNE     EV
Sbjct: 121 EYGSKMTDISRRIVKLLLKILGDNFENKFYDSEFSNCDGYLRMNNYSPQDSNENVKIEEV 180

Query: 181 EGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRS 240
           EGLGKHTDISCVTILFQD +GGLQMRSK GEWMDI+PCE+ALVVNIGDFM AWSNE+LRS
Sbjct: 181 EGLGKHTDISCVTILFQDHVGGLQMRSKEGEWMDIKPCEDALVVNIGDFMHAWSNEKLRS 240

Query: 241 AEHRVVLRQN-VKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRAT-- 300
           AEHRVVLRQN VKRFSM F WS+ DD+EIYAPSEVVGEGN RLY+ F TKEYR+FRAT  
Sbjct: 241 AEHRVVLRQNSVKRFSMVFIWSFEDDKEIYAPSEVVGEGNARLYKPFLTKEYRIFRATPR 300

Query: 301 GARGTYEKVGANVKEFAG 309
           GAR  YEKVGANV +FAG
Sbjct: 301 GARENYEKVGANVTDFAG 318

[Close]

BLAST of MC10g0231 vs. NCBI nr
Match: XP_031265650.1 (gibberellin 20-oxidase-like protein [Pistacia vera])

HSP 1 Score: 398 bits (1022), Expect = 5.22e-136
Identity = 194/315 (61.59%), Postives = 243/315 (77.14%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           M+ESQT VELPV+D+SQPLS S L +L AAC EWGFFYITNHGVS + Y+KL S+++ LF
Sbjct: 1   MAESQTTVELPVLDISQPLSTSKLSALSAACKEWGFFYITNHGVSKDLYRKLHSLSNHLF 60

Query: 61  RLPQDTKIKVG----ISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSN 120
            LP +TK+KVG    I +Y P F ASPFFESF++SGPDFS+SAQ   EALL   N+ FS 
Sbjct: 61  NLPYETKVKVGPFSSIKTYTPHFIASPFFESFRVSGPDFSSSAQSSVEALLKEPNSDFSE 120

Query: 121 LLEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDS--NEEV 180
            L+EYG KMT+LSK IV+ +L  LG +F+ KFY+ EF  C GYLRI NYSP ++   ++V
Sbjct: 121 TLKEYGRKMTELSKAIVQFVLMSLGKDFQKKFYESEFSKCHGYLRIINYSPPENVVEKQV 180

Query: 181 EGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRS 240
           EGLG HTD+SCVTI++QD++GGLQ+RSK G WMDI PCE+ LVVNIGD M AWSN RLRS
Sbjct: 181 EGLGMHTDMSCVTIVYQDEIGGLQVRSKEGTWMDINPCEDTLVVNIGDLMHAWSNGRLRS 240

Query: 241 AEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGAR 300
           +EHRVVL++N  RFS+AFFW + D++ I AP EVVGE + R+Y+TF  K+Y  FR +  +
Sbjct: 241 SEHRVVLKRNANRFSLAFFWCFEDEKNISAPDEVVGEEDNRVYKTFICKDYLRFRESSEK 300

Query: 301 GTYEKVGANVKEFAG 309
           G +EKVG  VK+FAG
Sbjct: 301 GKFEKVGFTVKQFAG 315

[Close]

BLAST of MC10g0231 vs. ExPASy TrEMBL
Match: A0A6J1CI26 (gibberellin 20-oxidase-like protein isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011697 PE=3 SV=1)

HSP 1 Score: 631 bits (1627), Expect = 1.50e-228
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF
Sbjct: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60

Query: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120
           RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE
Sbjct: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120

Query: 121 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGLGKH 180
           YGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGLGKH
Sbjct: 121 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGLGKH 180

Query: 181 TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV 240
           TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV
Sbjct: 181 TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV 240

Query: 241 LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV 300
           LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV
Sbjct: 241 LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV 300

Query: 301 GANVKEFAG 309
           GANVKEFAG
Sbjct: 301 GANVKEFAG 309

[Close]

BLAST of MC10g0231 vs. ExPASy TrEMBL
Match: A0A6J1CJY7 (gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC111011696 PE=3 SV=1)

HSP 1 Score: 524 bits (1349), Expect = 4.21e-186
Identity = 260/314 (82.80%), Postives = 276/314 (87.90%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           MSESQTCVELPVID+ + LSES L SL  ACNEWGFFYI NHGVS EFY +LISIADELF
Sbjct: 1   MSESQTCVELPVIDVYEDLSESALSSLTTACNEWGFFYIRNHGVSKEFYHRLISIADELF 60

Query: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120
           RLPQ+TK KVGIS YVPRF ASPFFESFK +GPDFSASA  LTEAL  H NT+FSNLLEE
Sbjct: 61  RLPQETKNKVGISCYVPRFMASPFFESFKFAGPDFSASAYSLTEALFDHPNTEFSNLLEE 120

Query: 121 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSN-----EEVE 180
           YGSKM +LSKRIV+LLLK+L D FENKFYD EF NC G L INNYSPRDSN     EE+E
Sbjct: 121 YGSKMNNLSKRIVELLLKILVDKFENKFYDSEFSNCKGNLGINNYSPRDSNKEDKVEEIE 180

Query: 181 GLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSA 240
           GLGKHTDISCVTILFQD +GGLQMRSKGGEWMDI+PCE+ALVVNIGDFMQAWSNERLRSA
Sbjct: 181 GLGKHTDISCVTILFQDNVGGLQMRSKGGEWMDIKPCEDALVVNIGDFMQAWSNERLRSA 240

Query: 241 EHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARG 300
           EHRVVLRQNVKRFSM+FFWSYVDD EIYAPSEVVGEGNVRLYR FS KEY+MFRAT ARG
Sbjct: 241 EHRVVLRQNVKRFSMSFFWSYVDDREIYAPSEVVGEGNVRLYRPFSIKEYKMFRATSARG 300

Query: 301 TYEKVGANVKEFAG 309
            YEKVGA VK+FAG
Sbjct: 301 NYEKVGATVKDFAG 314

[Close]

BLAST of MC10g0231 vs. ExPASy TrEMBL
Match: A0A6J1CHI8 (gibberellin 20-oxidase-like protein isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011697 PE=3 SV=1)

HSP 1 Score: 516 bits (1329), Expect = 7.24e-184
Identity = 263/309 (85.11%), Postives = 263/309 (85.11%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF
Sbjct: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60

Query: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLEE 120
           RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQF      
Sbjct: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQF------ 120

Query: 121 YGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGLGKH 180
                                                   RINNYSPRDSNEEVEGLGKH
Sbjct: 121 ----------------------------------------RINNYSPRDSNEEVEGLGKH 180

Query: 181 TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV 240
           TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV
Sbjct: 181 TDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEHRVV 240

Query: 241 LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV 300
           LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV
Sbjct: 241 LRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGTYEKV 263

Query: 301 GANVKEFAG 309
           GANVKEFAG
Sbjct: 301 GANVKEFAG 263

[Close]

BLAST of MC10g0231 vs. ExPASy TrEMBL
Match: A0A6J1CI97 (gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC111011430 PE=3 SV=1)

HSP 1 Score: 492 bits (1267), Expect = 1.50e-173
Identity = 248/318 (77.99%), Postives = 274/318 (86.16%), Query Frame = 0


Query: 1   MSESQT-CVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADEL 60
           MSES++  VELPVID+ + LSES L SL  AC+EWGFFYI NHGVS EFYQKLISIAD+L
Sbjct: 1   MSESKSYTVELPVIDVYEDLSESALSSLSEACSEWGFFYIRNHGVSKEFYQKLISIADQL 60

Query: 61  FRLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSNLLE 120
           F LP +TK KVG S Y+PRF ASPF+ESFK SGPDFSASA  LTE LLG H+ +FSNLL+
Sbjct: 61  FCLPHETKNKVGFSCYIPRFIASPFYESFKFSGPDFSASAHHLTETLLGKHDAEFSNLLK 120

Query: 121 EYGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNE-----EV 180
           EYGSKMTD+S+RIVKLLLK+LGDNFENKFYD EF NCDGYLR+NNYSP+DSNE     EV
Sbjct: 121 EYGSKMTDISRRIVKLLLKILGDNFENKFYDSEFSNCDGYLRMNNYSPQDSNENVKIEEV 180

Query: 181 EGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRS 240
           EGLGKHTDISCVTILFQD +GGLQMRSK GEWMDI+PCE+ALVVNIGDFM AWSNE+LRS
Sbjct: 181 EGLGKHTDISCVTILFQDHVGGLQMRSKEGEWMDIKPCEDALVVNIGDFMHAWSNEKLRS 240

Query: 241 AEHRVVLRQN-VKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRAT-- 300
           AEHRVVLRQN VKRFSM F WS+ DD+EIYAPSEVVGEGN RLY+ F TKEYR+FRAT  
Sbjct: 241 AEHRVVLRQNSVKRFSMVFIWSFEDDKEIYAPSEVVGEGNARLYKPFLTKEYRIFRATPR 300

Query: 301 GARGTYEKVGANVKEFAG 309
           GAR  YEKVGANV +FAG
Sbjct: 301 GARENYEKVGANVTDFAG 318

[Close]

BLAST of MC10g0231 vs. ExPASy TrEMBL
Match: A0A5N6QMY6 (Fe2OG dioxygenase domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_003930 PE=3 SV=1)

HSP 1 Score: 391 bits (1004), Expect = 1.53e-133
Identity = 188/314 (59.87%), Postives = 239/314 (76.11%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           MSESQT VELP++D+SQPL  S L SL   C +WGFF+I NHG+S +FY+KL  ++  LF
Sbjct: 1   MSESQTSVELPILDISQPLDPSFLSSLSEGCKKWGFFHIINHGISIDFYRKLYLLSKGLF 60

Query: 61  RLPQDTKIKVG----ISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGHHNTQFSN 120
            LP DTKIK+G    I +Y P F ASP+FES ++SGP+F ASAQ   + L    +++FS 
Sbjct: 61  SLPSDTKIKLGPFSSIKTYTPHFIASPYFESLRVSGPNFFASAQSSADVLFAQQSSEFSV 120

Query: 121 LLEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNE-EVE 180
           +L+E GSKM +LSK I+K+LL  LGD FE K+Y+ EFKNC GYLRINNYSP +S E EVE
Sbjct: 121 ILQECGSKMAELSKTILKILLLSLGDGFEEKYYESEFKNCHGYLRINNYSPPESLEDEVE 180

Query: 181 GLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSA 240
           GLG HTD+SCVTI+ QD++GGLQ+RSK G+WMDIRPCE  LVVNIGD +QAWSNE+ RS+
Sbjct: 181 GLGMHTDMSCVTIVCQDEIGGLQVRSKEGKWMDIRPCEGTLVVNIGDMLQAWSNEKFRSS 240

Query: 241 EHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARG 300
           EHRV+L+Q   RFS+AFFW + D++ I AP EVVGEGN++LY+ F   +Y  FR +  RG
Sbjct: 241 EHRVILKQPANRFSLAFFWCFEDEKMIMAPDEVVGEGNMKLYKPFVCLDYLKFRESNERG 300

Query: 301 TYEKVGANVKEFAG 309
            +EKVG  V++FAG
Sbjct: 301 KFEKVGFTVRDFAG 314

[Close]

BLAST of MC10g0231 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 354.0 bits (907), Expect = 1.2e-97
Identity = 167/315 (53.02%), Postives = 225/315 (71.43%), Query Frame = 0


Query: 1   MSESQTCVELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELF 60
           MSE  + ++LPV+DL+QP+  SIL SL  AC EWGFFY+TNHG+S E + K+ S++ ++F
Sbjct: 1   MSELHSSLQLPVLDLTQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLSRDVF 60

Query: 61  RLPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALL-GHHNTQFSNLLE 120
           + P ++K+K+G  SY PR+ ASP+FES  +SGPDFS SA+   + L   HH  +    ++
Sbjct: 61  KAPLESKLKLGPISYTPRYIASPYFESLVVSGPDFSDSAKASADVLFQDHHKPELRETMQ 120

Query: 121 EYGSKMTDLSKRIVKLLLKV-LGDNFENKFYDYEFKNCDGYLRINNYSP----RDSNEEV 180
           EYG+KM +LSKR++K+LL + LGD    + Y  +F NC GYLR+ NY+P        E V
Sbjct: 121 EYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEELV 180

Query: 181 EGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRS 240
           EGLG HTD+SC+TI++QD +GGLQMRSK G+W+DI PC + LVVNIGD MQAWSN RLRS
Sbjct: 181 EGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCNDFLVVNIGDLMQAWSNGRLRS 240

Query: 241 AEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGAR 300
           +EHRVVLR+ V R S+AFF  + D++ I AP E+VGEG  R Y++F   EY  FR +   
Sbjct: 241 SEHRVVLRKLVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQSNEE 300

Query: 301 GTYEKVGANVKEFAG 310
           G +EK+G  VK+FAG
Sbjct: 301 GKFEKIGFTVKDFAG 315

[Close]

BLAST of MC10g0231 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 313.5 bits (802), Expect = 1.8e-85
Identity = 158/318 (49.69%), Postives = 221/318 (69.50%), Query Frame = 0


Query: 8   VELPVIDLSQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELFRLPQDTK 67
           +ELPV D+S+PLSES L SL  AC EWGFFY+TNHGVS + Y+KL   +  +F L  + K
Sbjct: 3   LELPVFDISKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDEEK 62

Query: 68  IKVGISSYVPRFTASPFFESFKLSGPDFSASAQLLTEALLGH-HNTQFSNLLEEYGSKMT 127
           +K+G S+Y PRF ASPFFES ++SGPDF ASA+   +A      + +FS L++EYG KMT
Sbjct: 63  MKMGASNYTPRFIASPFFESLRVSGPDFYASAKSSVDAFSDQATDEEFSGLMKEYGEKMT 122

Query: 128 DLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYS-PRDSNEE---------VEG 187
            L ++I+K +L   GD+  +K+Y+ EF NC GY RINNY+ P D  ++         +EG
Sbjct: 123 KLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGDEQDLIEG 182

Query: 188 LGKHTDISCVTILFQDKLGGLQMRSKGG-EWMDIRPCENALVVNIGDFMQAWSNERLRSA 247
           LG HTD+SC+TI+ QD +GGLQ+R++ G   MDI P + ALVVN+GD + AW+N RLRS+
Sbjct: 183 LGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLLHAWTNGRLRSS 242

Query: 248 EHRVVLRQN---VKRFSMAFFWSYVDDEEIYAPSEVVG--EGNVRLYRTFSTKEYRMFRA 307
           +HRV+L++      RFS+AFFW + D + ++AP EVVG  EG +R++R+F   +Y  FR 
Sbjct: 243 QHRVILKRRGFVGNRFSLAFFWCFDDGKVVFAPDEVVGGCEG-MRVFRSFKCGDYLRFRE 302

Query: 308 TGARGTYEKVGANVKEFA 309
           +  +G +EKVG  V++FA
Sbjct: 303 SNEKGKFEKVGDTVEDFA 319

[Close]

BLAST of MC10g0231 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 232.6 bits (592), Expect = 4.1e-61
Identity = 110/197 (55.84%), Postives = 143/197 (72.59%), Query Frame = 0


Query: 118 LEEYGSKMTDLSKRIVKLLLKV-LGDNFENKFYDYEFKNCDGYLRINNYSP----RDSNE 177
           ++EYG+KM +LSKR++K+LL + LGD    + Y  +F NC GYLR+ NY+P        E
Sbjct: 1   MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEE 60

Query: 178 EVEGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERL 237
            VEGLG HTD+SC+TI++QD +GGLQMRSK G+W+DI PC + LVVNIGD MQAWSN RL
Sbjct: 61  LVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCNDFLVVNIGDLMQAWSNGRL 120

Query: 238 RSAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRATG 297
           RS+EHRVVLR+ V R S+AFF  + D++ I AP E+VGEG  R Y++F   EY  FR + 
Sbjct: 121 RSSEHRVVLRKLVNRVSLAFFLCFEDEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQSN 180

Query: 298 ARGTYEKVGANVKEFAG 310
             G +EK+G  VK+FAG
Sbjct: 181 EEGKFEKIGFTVKDFAG 197

[Close]

BLAST of MC10g0231 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 150.2 bits (378), Expect = 2.7e-36
Identity = 98/310 (31.61%), Postives = 159/310 (51.29%), Query Frame = 0


Query: 8   VELPVIDLSQPLS-ESILCSLC-----AACNEWGFFYITNHGVSDEFYQKLISIADELFR 67
           ++LPVIDLS   S E +    C     AA  EWGFF I NHG+  + ++ ++    +LF 
Sbjct: 38  IKLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFD 97

Query: 68  LPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQ---------LLTE-ALLGHHN 127
            P   K++   S             S++   P  ++ AQ         +L+E + +    
Sbjct: 98  QPFSVKVRERFSDLSK--------NSYRWGNPSATSPAQYSVSEAFHIILSEVSRISDDR 157

Query: 128 TQFSNLLEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSN 187
                ++E Y  ++  +++ I ++L K +  N  +++++  F+  + +LR+N Y P    
Sbjct: 158 NNLRTIVETYVQEIARVAQMICEILGKQV--NVSSEYFENIFELENSFLRLNKYHPSVFG 217

Query: 188 EEVEGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNER 247
            EV GL  HTD S +TIL QD++GGL++ +  G+W+ ++PC  AL VNIGD  QA SN  
Sbjct: 218 SEVFGLVPHTDTSFLTILSQDQIGGLELEN-NGQWISVKPCLEALTVNIGDMFQALSNGV 277

Query: 248 LRSAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYRMFRAT 302
            +S  HRV+   N++R S+AFF         Y  +E+   G  + YR FS +EY+     
Sbjct: 278 YQSVRHRVISPANIERMSIAFFVC------PYLETEIDCFGYPKKYRRFSFREYKEQSEH 330

[Close]

BLAST of MC10g0231 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 146.7 bits (369), Expect = 3.0e-35
Identity = 98/294 (33.33%), Postives = 147/294 (50.00%), Query Frame = 0


Query: 8   VELPVIDLSQPLS------ESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELFR 67
           VELPVID+S+ +       E    ++  A  EWGFF + NHG+S +  +K+      +FR
Sbjct: 40  VELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFR 99

Query: 68  LPQDTKIKVGISSYVPRFTASPFFESFKLSGPDFSASAQL---------LTEALLGHHNT 127
            P D K K        +F+A     S++   P  ++  QL         +T+       T
Sbjct: 100 EPFDKKSK------SEKFSAG----SYRWGTPSATSIRQLSWSEAFHVPMTDISDNKDFT 159

Query: 128 QFSNLLEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNE 187
             S+ +E++ S+   L+  + ++L +  G N  + F+         YLR+N Y P     
Sbjct: 160 TLSSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYPPCPKPS 219

Query: 188 EVEGLGKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERL 247
           EV GL  HTD   +TIL+QD++GGLQ+  K   W+ ++P   AL++NIGD  QAWSN   
Sbjct: 220 EVYGLMPHTDSDFLTILYQDQVGGLQL-IKDNRWIAVKPNPKALIINIGDLFQAWSNGMY 279

Query: 248 RSAEHRVVLRQNVKRFSMAFFWSYVDDEEIYAPSEVVGEGNVRLYRTFSTKEYR 287
           +S EHRV+    V+RFS A+F     D  I   S+         YR FS +E+R
Sbjct: 280 KSVEHRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPA------YRNFSFREFR 314

The following BLAST results are available for this feature:

BLAST of MC10g0231 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KBY0	2.6e-84	49.69	Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310...	[more]
Q9C6I4	3.8e-35	31.61	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ...	[more]
O49561	4.2e-34	33.33	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
Q9C971	7.9e-33	31.62	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
Q8LGZ9	8.8e-32	31.49	Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]

BLAST of MC10g0231 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141254.1	6.26e-228	99.68	gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia]	[more]
XP_022141253.1	8.70e-186	82.80	gibberellin 20-oxidase-like protein [Momordica charantia]	[more]
XP_022141255.1	1.49e-183	85.11	gibberellin 20-oxidase-like protein isoform X2 [Momordica charantia]	[more]
XP_022140867.1	3.09e-173	77.99	gibberellin 20-oxidase-like protein [Momordica charantia]	[more]
XP_031265650.1	5.22e-136	61.59	gibberellin 20-oxidase-like protein [Pistacia vera]	[more]

BLAST of MC10g0231 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CI26	1.50e-228	100.00	gibberellin 20-oxidase-like protein isoform X1 OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1CJY7	4.21e-186	82.80	gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC1110116...	[more]
A0A6J1CHI8	7.24e-184	85.11	gibberellin 20-oxidase-like protein isoform X2 OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1CI97	1.50e-173	77.99	gibberellin 20-oxidase-like protein OS=Momordica charantia OX=3673 GN=LOC1110114...	[more]
A0A5N6QMY6	1.53e-133	59.87	Fe2OG dioxygenase domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH...	[more]

BLAST of MC10g0231 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23340.1	1.2e-97	53.02	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51310.1	1.8e-85	49.69	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G23340.2	4.1e-61	55.84	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G50960.1	2.7e-36	31.61	gibberellin 2-oxidase 7	[more]
AT4G21200.1	3.0e-35	33.33	gibberellin 2-oxidase 8	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 180..201 score: 37.6 coord: 216..242 score: 26.94 coord: 27..44 score: 53.54
None	No IPR available	PANTHER	PTHR47990:SF9	GIBBERELLIN 20-OXIDASE-LIKE PROTEIN	coord: 2..305
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 2..305
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 6..292
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 161..253 e-value: 1.7E-22 score: 79.8
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..288 e-value: 6.6E-73 score: 248.1
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 10..74 e-value: 2.1E-16 score: 60.8
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 155..256 score: 10.948321

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g0231.1	MC10g0231.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009987	cellular process
molecular_function	GO:0016706	2-oxoglutarate-dependent dioxygenase activity
molecular_function	GO:0046872	metal ion binding