MC10g0227 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g0227
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	DNA-directed RNA polymerase III subunit rpc4-like
Location	MC10: 1775979 .. 1780296 (-)
RNA-Seq Expression	MC10g0227
Synteny	MC10g0227

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTTCTCTCTTCTTTCTTTTTCCCCCTAAAAAAATGAAACTTATAATTTATAAAACAATAAAAAATTTAAATGTAAGTAATGAAAAATTCAAAATTATTTTTTGGCATTTCCCAGACAAATAATTATATATATTTGTATTTAAATTTGCAAGTTGGGGTCTTGTAAACAGCGTGTCTTCAGTGGCAAAACATCATTTCTTTCATCCGTCTGGTCTAGGGTTCGAACCTTCCCTTTTTTCATTTTCACCATTTTTCCCTTCCCTTTCCCTTGTCATCTTCAACCTCCAGCCATGAAGAATCCGGATCCTTCATCTCGTCCACGCAGAAAGGTGATTTTTTATTTTCCTTAAATCTCGTTTTCTTCTACTTCGATTTAGATCTGAACACTCGTTTTTGCAGGTTAAATTTGCCCCAAAACCCACACCGCGCAAAAGGCCACCCCCCGCGCCAAAACCGTACTCCTCTGAATTTAATTCCTCATAGAGTTTTCCTTTTCCTTCTCCATATTTTGGCTTCTGGATTCGCAAAAAGTTACAGTAATCAATCTTGTTGTTTTGCCTAATCTCTTCCAGCGAAGACGAAGACGGCAATCAAAATGCTGCCCAAACTCGGTATCTCTTGCGCCGCGCCACCGTAAGCTCCTTCGTTCTTTCTGTGATTTTCGAATTCTTGTTTGCTTTTACATAATCTCTCTGAGTTTACCTTTGGAGAGATCAGATTTGTGGATAAATATTAAAGCGTTTGAATTTTCCCACTTGCAAGCGACGTTTTGGGTTTACTACAAGTTTCCGTTTGCTTCCCGATAAAAAAATCAAAAAAAAAAGAAAAAAAGAAAAAAATCGATGGAAATGAATTTCGTTTTCACCAACGCAGCGTTTGCGTTTCCCTTTCTTGTGAGATGAAAATGAAATTAATTTTGGAGGACATTTGTGTGTTTACGGTTTACACGTGTATGGCTCGACTTTGAGCCTTTCTTAAATTAACTTTTAAGACTTGTCTTTTTTTTAATACATTTTTTGTTCTAATATTTTATTACGGTACTTTCAAAACGTTTATTTTAATTTTTTTAAATTTTTTATCTAATTTTTATATTTTTAAAATGTTGATTTTAGTATTTATACATGATCTTCATTTTAGTTTTTATCTCATTTATTTCTAGCAAAATTCCAAATAAGATTTTATGTTCTAAAATTCTTGAAATGTAGTCATAAATCTACGTAGAATTTATATTAAAATTTTGTATAATCACTTTTTAAAAGTACGGAAACCAAATATGATGTTTTGAAATTGTATGAAGTAAGATAAACAAAAGTTGAAAAGACATTGCCAGCACCAAAAAGTATTTAAACAATTTTTTATATTGTTTTTTTTTTCATTTTTAACTTAAAAGGCAAAAATCTATATATTTATATAACAGTAATAGTAGTATAGATATGTTTTTGCCTTAAAAAAAAAGAAGTAATAATAGAAACATGTTTTCTTTCTTTGACGACTTGATCTTTTTGTTTCTTTTTTTAAATATTGTTGTACTTTGTACTTAGTTTAATATCTAAACTTTTATTTTAAATTGATTTTTGTGGCATTAGAGCTAACTATTTGACTTATAATCCATGAATGGATGCTTAATCACTTCAACTATACCAATCCAATGTGTGAAACAAATTTGTCTAGTTGTATCGAACATTTCGTTGCTAAATTTTAGTTTTTTTTTTTTTTTTGGCAATTTGATTTTGGATGAGTTTGAATATTTTTTTAGTTTATTTGACATTTTCTTTATTTACTAACATGATTGTATATAAAACATGTAATGACTCTATCCCCAAATGATGGATATATAATGAAAAGGGCAACAATCGACATTCTTCATTTGGTCTTAATACTGATATTGATTTGGTTTTTTTCCAATTTACAGGAGAATCTTGGAAAACGTGGGACCAAGGTCGAGAAAAAACGTAAAGTCTTCATTCATCAAGATTTTTCGACTGATATAAAGAACTCTGTATTTAATCGATTAATTTTCATTCGTAAAAGAGTCACAGATAAGGATAAGATTGAGAAAATAAAATCCCAAGTTTCTAGCAAGGGAAGATTTAAGTAAATATGATTTTACTTGCTATGACTGATCAACAGAGACGAGGATAAGATGAAGTTTCCATTTTTGCCTTACTATGATCTCCATTTTCATGTTTATATCTGCAGCTTCAGTCCAAGTTGCATTTGGTCCCGGAGCAGCATCAGCATCATCTTCCATAAGAACATATGGCGTTCCCAAAGTGGGGAACAGTAGTAGAAAAAATAACATTGAGCCTGAAATTTCTGACGATGAGGAATCTATCTTGCCTCTCCCTGTGGATGTTGAAGAAGATGGGATGATTTTCGATCAAAAAAGCGAAGATGGAACACCCGAATCCGCCATGGCTACCAATGCTGTGGTACTAACCAAGGCCAAGAAGGATTACAGAGAACCTTGGGTAATCTCTCCCTCTCTTTCTCTATCTCGCCTTTGCGGCACACACTTATAGATTAACATCCCGATTTCGAGATGCTTTTCAGGATTACCATCATTCTTACTATCCCAATACGCTTCCTTTACGGAAGCCTTACTCCGGACACCCAGGTATGTCTCTATTTCTTGGTTCACTATTAGTTTTTGTTTGTGAAAAGTGATCTCTAGTGCTCAAAATCAACCTTGAAAACAAAAGCAGACTCCCAATAACTAATGACTATGGATGATTAATGGTGGATGAATCCTTGCTTATTTATGTTGCAATGAACATTTTACCTTGGTGGTGGATCATTCTGGCTTTGCTCTGTAATTGCTAACTTTCCAGCTTCTGATTTTAGAACTACTTGATAAAGCTGAATTCGGGCTGGATGCACCGGATAGGGAGTATGACGAGAACGCTGCAATACCTGCATTAGATCTAGAATTGCTGGTTGGTATTTGGATTGATAACTGTGGATTATTTCATATACTTTTTGGTAGTCTTTCTTCATTTAGAAACTAGTTATAATATGGTGGCCATTTGAAGCAGGAGGAAAAGGAGGAGAAGACTATGTACTTTTTTCAGCTTCCTGCCTGTCTTCCTTTACCCAAACAATCATCTACTGCAACAAGGAAGGAGAAAGTAGGAAGTTCGAGATCTTCGAACAGCAAAGGCACGTCAGAGCTCGGCGATTTGAAGAAATTGCCTGGTGGATATATGGGGAAACTACTGATATACAAGAGTGGAGCTGTTAAGTTGAGGCTTGGAGAAACTCTCTACGACGTGAGTAATATGTCATTGGCTTCCTATAAATCGAAATAGAGCATCAAGGAGTTCGTTTTTTGTTATTGTTCTTGATGTTTGGGGATTAGTTAGACCTTGTAATTTCTTTTTGGTTTCTCTTTTTGGGGCTTTTTGTTGCAGGTTTCTTCTGGTTCAAACTGCAGCTTTCTTCAACATGTTGTGGCAATCAACACCGAAGAGGGACACTGCTGTGATCTCGGAGATGTCGGAAAACGAGTCGTTGTGACGCCAGATATTAGTTCCCTGCTGAATGCAGTGACTGACTAAGGATTAATGTGAAAAACTATGCCTGTGGATAGAACAACCAAAAAGAAGAGAAGAAGGCAAAGGCTGCAATGTCAATAGATTTGATGGCTGGCTCTGGCTTAGGCTGTGTGTACTACTTGTAAGTGGAACTGCTGAAGATGCTGACTTGGTAAGGAAGTTTCATTTTGATGGTCTTAGGATGTGAATTTCTTGACCAAACTGCCTCCTTTGTTGATTCTAATACCAAAAAAAAAAAAAAACATATTTATTGTCTCTGATTTCTAGCTATGGAATAGATTGACAGATGACAATGTTGATATTGTAAATTTTCTCTTACTTATGATCTTCATTTGATGTTTGAGTTATTTTAAAATAACCCATTAACCATCTGAGGTTTTTTTATTTTATACGTGTGTCGGGCAGCTTACGCGCACCTCGACTATTCACGGGCCTATCGCCTATCATGCTATAGGATTAACTATCTGAGTTGTTGTAGTGCCAATAATAATTACGGTAAATAGTAAAGGATTTAGAATAAAAGGAGTTTGTACCATGGTGACCAGCTTAGGGTTGAATGGTCAAGTGAATTGGACGCTGGTTTTTGGAGCTCATCAGTGTATTTAACCCACTCAATACGGTTTAGCCTACCATGTATTGTGAAATTTTTTTTGGTATTATGTATGTACATATTTCATTTATTCACACCCTTAATTTTTCTTTATAGATAATTATTCAATACTACTAGTCCCCACATCATTCTTAAATGAAATAATAGATGATAC

mRNA sequence

TGTTCTCTCTTCTTTCTTTTTCCCCCTAAAAAAATGAAACTTATAATTTATAAAACAATAAAAAATTTAAATGTAAGTAATGAAAAATTCAAAATTATTTTTTGGCATTTCCCAGACAAATAATTATATATATTTGTATTTAAATTTGCAAGTTGGGGTCTTGTAAACAGCGTGTCTTCAGTGGCAAAACATCATTTCTTTCATCCGTCTGGTCTAGGGTTCGAACCTTCCCTTTTTTCATTTTCACCATTTTTCCCTTCCCTTTCCCTTGTCATCTTCAACCTCCAGCCATGAAGAATCCGGATCCTTCATCTCGTCCACGCAGAAAGGTTAAATTTGCCCCAAAACCCACACCGCGCAAAAGGCCACCCCCCGCGCCAAAACCCGAAGACGAAGACGGCAATCAAAATGCTGCCCAAACTCGGTATCTCTTGCGCCGCGCCACCGAGAATCTTGGAAAACGTGGGACCAAGGTCGAGAAAAAACCTTCAGTCCAAGTTGCATTTGGTCCCGGAGCAGCATCAGCATCATCTTCCATAAGAACATATGGCGTTCCCAAAGTGGGGAACAGTAGTAGAAAAAATAACATTGAGCCTGAAATTTCTGACGATGAGGAATCTATCTTGCCTCTCCCTGTGGATGTTGAAGAAGATGGGATGATTTTCGATCAAAAAAGCGAAGATGGAACACCCGAATCCGCCATGGCTACCAATGCTGTGGTACTAACCAAGGCCAAGAAGGATTACAGAGAACCTTGGGATTACCATCATTCTTACTATCCCAATACGCTTCCTTTACGGAAGCCTTACTCCGGACACCCAGAACTACTTGATAAAGCTGAATTCGGGCTGGATGCACCGGATAGGGAGTATGACGAGAACGCTGCAATACCTGCATTAGATCTAGAATTGCTGGAGGAAAAGGAGGAGAAGACTATGTACTTTTTTCAGCTTCCTGCCTGTCTTCCTTTACCCAAACAATCATCTACTGCAACAAGGAAGGAGAAAGTAGGAAGTTCGAGATCTTCGAACAGCAAAGGCACGTCAGAGCTCGGCGATTTGAAGAAATTGCCTGGTGGATATATGGGGAAACTACTGATATACAAGAGTGGAGCTGTTAAGTTGAGGCTTGGAGAAACTCTCTACGACGTTTCTTCTGGTTCAAACTGCAGCTTTCTTCAACATGTTGTGGCAATCAACACCGAAGAGGGACACTGCTGTGATCTCGGAGATGTCGGAAAACGAGTCGTTGTGACGCCAGATATTAGTTCCCTGCTGAATGCAGTGACTGACTAAGGATTAATGTGAAAAACTATGCCTGTGGATAGAACAACCAAAAAGAAGAGAAGAAGGCAAAGGCTGCAATGTCAATAGATTTGATGGCTGGCTCTGGCTTAGGCTGTGTGTACTACTTGTAAGTGGAACTGCTGAAGATGCTGACTTGGTAAGGAAGTTTCATTTTGATGGTCTTAGGATGTGAATTTCTTGACCAAACTGCCTCCTTTGTTGATTCTAATACCAAAAAAAAAAAAAAACATATTTATTGTCTCTGATTTCTAGCTATGGAATAGATTGACAGATGACAATGTTGATATTGTAAATTTTCTCTTACTTATGATCTTCATTTGATGTTTGAGTTATTTTAAAATAACCCATTAACCATCTGAGGTTTTTTTATTTTATACGTGTGTCGGGCAGCTTACGCGCACCTCGACTATTCACGGGCCTATCGCCTATCATGCTATAGGATTAACTATCTGAGTTGTTGTAGTGCCAATAATAATTACGGTAAATAGTAAAGGATTTAGAATAAAAGGAGTTTGTACCATGGTGACCAGCTTAGGGTTGAATGGTCAAGTGAATTGGACGCTGGTTTTTGGAGCTCATCAGTGTATTTAACCCACTCAATACGGTTTAGCCTACCATGTATTGTGAAATTTTTTTTGGTATTATGTATGTACATATTTCATTTATTCACACCCTTAATTTTTCTTTATAGATAATTATTCAATACTACTAGTCCCCACATCATTCTTAAATGAAATAATAGATGATAC

Coding sequence (CDS)

ATGAAGAATCCGGATCCTTCATCTCGTCCACGCAGAAAGGTTAAATTTGCCCCAAAACCCACACCGCGCAAAAGGCCACCCCCCGCGCCAAAACCCGAAGACGAAGACGGCAATCAAAATGCTGCCCAAACTCGGTATCTCTTGCGCCGCGCCACCGAGAATCTTGGAAAACGTGGGACCAAGGTCGAGAAAAAACCTTCAGTCCAAGTTGCATTTGGTCCCGGAGCAGCATCAGCATCATCTTCCATAAGAACATATGGCGTTCCCAAAGTGGGGAACAGTAGTAGAAAAAATAACATTGAGCCTGAAATTTCTGACGATGAGGAATCTATCTTGCCTCTCCCTGTGGATGTTGAAGAAGATGGGATGATTTTCGATCAAAAAAGCGAAGATGGAACACCCGAATCCGCCATGGCTACCAATGCTGTGGTACTAACCAAGGCCAAGAAGGATTACAGAGAACCTTGGGATTACCATCATTCTTACTATCCCAATACGCTTCCTTTACGGAAGCCTTACTCCGGACACCCAGAACTACTTGATAAAGCTGAATTCGGGCTGGATGCACCGGATAGGGAGTATGACGAGAACGCTGCAATACCTGCATTAGATCTAGAATTGCTGGAGGAAAAGGAGGAGAAGACTATGTACTTTTTTCAGCTTCCTGCCTGTCTTCCTTTACCCAAACAATCATCTACTGCAACAAGGAAGGAGAAAGTAGGAAGTTCGAGATCTTCGAACAGCAAAGGCACGTCAGAGCTCGGCGATTTGAAGAAATTGCCTGGTGGATATATGGGGAAACTACTGATATACAAGAGTGGAGCTGTTAAGTTGAGGCTTGGAGAAACTCTCTACGACGTTTCTTCTGGTTCAAACTGCAGCTTTCTTCAACATGTTGTGGCAATCAACACCGAAGAGGGACACTGCTGTGATCTCGGAGATGTCGGAAAACGAGTCGTTGTGACGCCAGATATTAGTTCCCTGCTGAATGCAGTGACTGACTAA

Protein sequence

MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGTKVEKKPSVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFGLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD

Homology

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BLAST of MC10g0227 vs. ExPASy Swiss-Prot
Match: P05423 (DNA-directed RNA polymerase III subunit RPC4 OS=Homo sapiens OX=9606 GN=POLR3D PE=1 SV=2)

HSP 1 Score: 62.4 bits (150), Expect = 1.1e-08
Identity = 54/169 (31.95%), Postives = 80/169 (47.34%), Query Frame = 0


Query: 181 DKAEFGLDAPDREYDENAAIP--ALDLELLEE---KEEKTMYFFQLPACLP--LPKQSST 240
           ++ E  + AP +   +   +P      ELL E    +E+ + F QLP  LP   P Q   
Sbjct: 226 EEEEAKMKAPPKAARKTPGLPKDVSVAELLRELSLTKEEELLFLQLPDTLPGQPPTQDIK 285

Query: 241 ATRKEKVG---------SSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETL 300
             + E  G           +   +K       L  L  G +GKLLI KSG V+L LG+  
Sbjct: 286 PIKTEVQGEDGQVVLIKQEKDREAKLAENACTLADLTEGQVGKLLIRKSGRVQLLLGKVT 345

Query: 301 YDVSSGSNCSFLQHVVAI---NTEEGHCCDLGDVGKRVVVTPDISSLLN 331
            DV+ G+ CSFLQ +V++   ++  G    LG V  ++V +PD  SLL+
Sbjct: 346 LDVTMGTACSFLQELVSVGLGDSRTGEMTVLGHVKHKLVCSPDFESLLD 394

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BLAST of MC10g0227 vs. ExPASy Swiss-Prot
Match: Q91WD1 (DNA-directed RNA polymerase III subunit RPC4 OS=Mus musculus OX=10090 GN=Polr3d PE=2 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 3.3e-08
Identity = 67/252 (26.59%), Postives = 111/252 (44.05%), Query Frame = 0


Query: 101 EPEISDDEESILPLPVDVEEDGMIFDQKSED--------GTPESAMATNAVVLTKAKKDY 160
           +P + +D  + +P+ + +   G +F ++SE+        G  E  M  + V   K K++ 
Sbjct: 166 DPGLKNDTRN-MPVQLPLAHSGWLFKEESEEPEAKPFSAGPKEEDMEVD-VPAVKVKEEP 225

Query: 161 REPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFGLDAPDREYDENAAIPALDLELLEEKE 220
           R+  +      P     + P  G P+ +  AE                   +L L++++E
Sbjct: 226 RDEEEEAKVKAPPRAARKTP--GLPKDVSVAEL----------------LRELSLMKDEE 285

Query: 221 EKTMYFFQLPACLP--LPKQSSTATRKEKVG---------SSRSSNSKGTSELGDLKKLP 280
              + F QLP  LP   P Q     + E  G           +   ++       L  L 
Sbjct: 286 ---LLFLQLPDTLPGQPPTQDIKPVKTEVQGEDGQMVVIKQEKDREARLAENACTLADLT 345

Query: 281 GGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAI---NTEEGHCCDLGDVGKR 331
            G +GKLLI KSG V+L LG+   DV+ G+ CSFLQ +V++   ++  G    LG V  +
Sbjct: 346 EGQVGKLLIRKSGKVQLLLGKVTLDVTMGTTCSFLQELVSVGLGDSRTGEMTVLGHVKHK 394

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BLAST of MC10g0227 vs. ExPASy Swiss-Prot
Match: Q5E9Z7 (DNA-directed RNA polymerase III subunit RPC4 OS=Bos taurus OX=9913 GN=POLR3D PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.2e-07
Identity = 49/145 (33.79%), Postives = 73/145 (50.34%), Query Frame = 0


Query: 206 ELLEE---KEEKTMYFFQLPACLP-LPKQSSTATRKEKVGSS-------------RSSNS 265
           ELL E    +E+ + F QLP  LP  P        K +V S               +  +
Sbjct: 253 ELLRELSLTQEEELLFLQLPDSLPGQPPTQDIKPIKTEVQSEDGQMVVIKQEKDREARLA 312

Query: 266 KGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAI---NTE 325
           + T  L DL +   G +GKLLI KSG V+L LG+   DV+ G+ CSFLQ +V++   ++ 
Sbjct: 313 ENTCTLADLTE---GQVGKLLIRKSGKVQLLLGKVTLDVTMGTTCSFLQELVSVGLGDSR 372

Query: 326 EGHCCDLGDVGKRVVVTPDISSLLN 331
            G    LG +  ++V +P+  SLL+
Sbjct: 373 TGDMTVLGHIKHKLVCSPNFESLLD 394

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BLAST of MC10g0227 vs. ExPASy Swiss-Prot
Match: Q8T1V7 (DNA-directed RNA polymerase III subunit rpc4 OS=Dictyostelium discoideum OX=44689 GN=polr3d PE=3 SV=2)

HSP 1 Score: 53.5 bits (127), Expect = 5.2e-06
Identity = 29/77 (37.66%), Postives = 41/77 (53.25%), Query Frame = 0


Query: 257 LKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTEEG-----HCCD 316
           +K++P G +G L IYKSG   L++G   YD+SSG    FL+ V    T+ G      C  
Sbjct: 351 IKEMPSGKLGTLKIYKSGKSILKIGTVEYDISSGDKLKFLEEVQCFQTKGGPDNSPTCYT 410

Query: 317 LGDVGKRVVVTPDISSL 329
           LG   + +V  P IS +
Sbjct: 411 LGIPSQHLVAAPIISQI 427

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BLAST of MC10g0227 vs. ExPASy Swiss-Prot
Match: P25441 (DNA-directed RNA polymerase III subunit RPC4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RPC53 PE=1 SV=2)

HSP 1 Score: 50.4 bits (119), Expect = 4.4e-05
Identity = 38/131 (29.01%), Postives = 69/131 (52.67%), Query Frame = 0


Query: 205 LELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRSSNS--------KGTSELGD 264
           L+ +  K E+ M  FQLP  LP  ++ +    KE + +  S  S        K T +   
Sbjct: 294 LKKMNNKPERFM-VFQLPTRLPAFERPAVKEEKEDMETQASDPSKKKKNIKKKDTKDALS 353

Query: 265 LKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAIN-TEEGHCCD-LGD 324
            ++L  G +G + ++KSG + +++G  + D+  G+  +FLQ V+A++  ++    + LG 
Sbjct: 354 TREL-AGKVGSIRVHKSGKLSVKIGNVVMDIGKGAETTFLQDVIALSIADDASSAELLGR 413

Query: 325 VGKRVVVTPDI 326
           V  ++VVTP I
Sbjct: 414 VDGKIVVTPQI 422

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BLAST of MC10g0227 vs. NCBI nr
Match: XP_022141051.1 (DNA-directed RNA polymerase III subunit RPC4-like isoform X2 [Momordica charantia])

HSP 1 Score: 653 bits (1684), Expect = 3.98e-236
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0


Query: 1   MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT 60
           MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT
Sbjct: 1   MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT 60

Query: 61  KVEKKPSVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEE 120
           KVEKKPSVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEE
Sbjct: 61  KVEKKPSVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEE 120

Query: 121 DGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELL 180
           DGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELL
Sbjct: 121 DGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELL 180

Query: 181 DKAEFGLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKV 240
           DKAEFGLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKV
Sbjct: 181 DKAEFGLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKV 240

Query: 241 GSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVV 300
           GSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVV
Sbjct: 241 GSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVV 300

Query: 301 AINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD 334
           AINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD
Sbjct: 301 AINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD 334

[Close]

BLAST of MC10g0227 vs. NCBI nr
Match: XP_022141050.1 (DNA-directed RNA polymerase III subunit RPC4-like isoform X1 [Momordica charantia])

HSP 1 Score: 648 bits (1672), Expect = 2.79e-234
Identity = 334/335 (99.70%), Postives = 334/335 (99.70%), Query Frame = 0


Query: 1   MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT 60
           MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT
Sbjct: 1   MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT 60

Query: 61  KVEKKPSVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEE 120
           KVEKKPSVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEE
Sbjct: 61  KVEKKPSVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEE 120

Query: 121 DGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELL 180
           DGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELL
Sbjct: 121 DGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELL 180

Query: 181 DKAEFGLDAPDREYDENAAIPALDLELL-EEKEEKTMYFFQLPACLPLPKQSSTATRKEK 240
           DKAEFGLDAPDREYDENAAIPALDLELL EEKEEKTMYFFQLPACLPLPKQSSTATRKEK
Sbjct: 181 DKAEFGLDAPDREYDENAAIPALDLELLQEEKEEKTMYFFQLPACLPLPKQSSTATRKEK 240

Query: 241 VGSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHV 300
           VGSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHV
Sbjct: 241 VGSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHV 300

Query: 301 VAINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD 334
           VAINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD
Sbjct: 301 VAINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD 335

[Close]

BLAST of MC10g0227 vs. NCBI nr
Match: XP_038879339.1 (DNA-directed RNA polymerase III subunit rpc4-like [Benincasa hispida])

HSP 1 Score: 485 bits (1248), Expect = 9.57e-170
Identity = 253/334 (75.75%), Postives = 289/334 (86.53%), Query Frame = 0


Query: 1   MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT 60
           MKNPD SS PRRKVKFAPK  PR+RPPP  K EDEDG+   AQTRYLLRRATENLGKRG 
Sbjct: 1   MKNPD-SSPPRRKVKFAPKSAPRRRPPPVQKIEDEDGDGYVAQTRYLLRRATENLGKRGN 60

Query: 61  KVEKKPSVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEE 120
           KV++K SVQVAFGPGAAS SSSIRTYGVPKV NSSRKN+IEP++ +DEE ILPLP+DVEE
Sbjct: 61  KVQRKSSVQVAFGPGAASTSSSIRTYGVPKVENSSRKNDIEPDVDEDEEFILPLPMDVEE 120

Query: 121 DGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELL 180
           +G IFD+K++DG  ES+   ++ ++TK KKDY+EPWDYH+S YP TLP+R PYSG PE L
Sbjct: 121 NGKIFDKKTKDGIAESS---SSAIVTKTKKDYKEPWDYHNSNYPTTLPIRMPYSGDPEQL 180

Query: 181 DKAEFGLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKV 240
           D+AEFG DA DREYDE++ IPALDL LL+EK E T YFFQLPA LPLPKQSSTAT KEKV
Sbjct: 181 DEAEFGQDATDREYDEDSVIPALDLGLLDEKTENTKYFFQLPARLPLPKQSSTATGKEKV 240

Query: 241 GSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVV 300
           G+ RSS S G+SELGDLKKL  G MGKLLIYKSGA+KLRLG+TLYDVSSGS+CSFLQHVV
Sbjct: 241 GNLRSSKSMGSSELGDLKKLSAGCMGKLLIYKSGAIKLRLGDTLYDVSSGSDCSFLQHVV 300

Query: 301 AINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD 334
           AINT+EG CCDLGD+GKRV+VTPDISSLLN++T+
Sbjct: 301 AINTKEGQCCDLGDIGKRVIVTPDISSLLNSMTN 330

[Close]

BLAST of MC10g0227 vs. NCBI nr
Match: KAG6576814.1 (DNA-directed RNA polymerase III subunit RPC4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 470 bits (1210), Expect = 4.35e-164
Identity = 249/336 (74.11%), Postives = 279/336 (83.04%), Query Frame = 0


Query: 1   MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT 60
           MKNPDPS  PRRK+KFAPK TPR+RPPPAPK EDED   N AQTRYLLRRATENLGKRG 
Sbjct: 1   MKNPDPSP-PRRKIKFAPKSTPRRRPPPAPKTEDEDVVGNVAQTRYLLRRATENLGKRGN 60

Query: 61  KVEKKPSVQVAFGPGAASAS--SSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDV 120
           KVEKK SVQVAFGPGAASAS  SSIRTYGVPK+GNSSRK +IEPE ++DEE ILPLP+D 
Sbjct: 61  KVEKKSSVQVAFGPGAASASESSSIRTYGVPKIGNSSRKKDIEPESAEDEEHILPLPMDF 120

Query: 121 EEDGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPE 180
           EE G   ++K+ED T             K  KDY+EPWDYH+SYYP TLPLR PYSG PE
Sbjct: 121 EEYGKNLNEKTEDAT-------------KNTKDYKEPWDYHNSYYPTTLPLRMPYSGDPE 180

Query: 181 LLDKAEFGLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKE 240
           +LD+AEFG D  DREYDEN++IPALDL LL+E  E T YFFQLPACLP PK+SST+T KE
Sbjct: 181 MLDEAEFGQDVMDREYDENSSIPALDLGLLDENSENTRYFFQLPACLPFPKRSSTSTGKE 240

Query: 241 KVGSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQH 300
           KVG+ RSS S  +SELGDL+ LPGGYMGKLLIYKSGAVKLRLG+TLYDVSSG +CSFLQH
Sbjct: 241 KVGNLRSSISTASSELGDLQNLPGGYMGKLLIYKSGAVKLRLGDTLYDVSSGLDCSFLQH 300

Query: 301 VVAINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD 334
           VVAINT++G CCDLG++GKRVVVTPDI SLLN++TD
Sbjct: 301 VVAINTDKGQCCDLGEIGKRVVVTPDIGSLLNSMTD 322

[Close]

BLAST of MC10g0227 vs. NCBI nr
Match: XP_022922968.1 (DNA-directed RNA polymerase III subunit rpc4-like [Cucurbita moschata])

HSP 1 Score: 468 bits (1203), Expect = 5.05e-163
Identity = 248/336 (73.81%), Postives = 278/336 (82.74%), Query Frame = 0


Query: 1   MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT 60
           MKNPDPS  PRRK+KFAPK TPR+RPPPAPK EDED   N AQTRYLLRRATENLGKRG 
Sbjct: 1   MKNPDPSP-PRRKIKFAPKSTPRRRPPPAPKTEDEDVVGNVAQTRYLLRRATENLGKRGN 60

Query: 61  KVEKKPSVQVAFGPGAASAS--SSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDV 120
           KVEKK SVQVAFGPGAASAS  SSIRTYGVPK+GNSSRK +IEPE ++DEE ILP P+D 
Sbjct: 61  KVEKKSSVQVAFGPGAASASESSSIRTYGVPKIGNSSRKKDIEPESAEDEELILPPPMDF 120

Query: 121 EEDGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPE 180
           EE G   ++K+ED T             K  KDY+EPWDYH+SYYP TLPLR PYSG PE
Sbjct: 121 EEYGKNLNEKTEDAT-------------KNTKDYKEPWDYHNSYYPTTLPLRMPYSGDPE 180

Query: 181 LLDKAEFGLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKE 240
           +LD+AEFG D  DREYDEN++IPALDL LL+E  E T YFFQLPACLP PK+SST+T KE
Sbjct: 181 MLDEAEFGQDVMDREYDENSSIPALDLGLLDENSENTRYFFQLPACLPFPKRSSTSTGKE 240

Query: 241 KVGSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQH 300
           KVG+ RSS S  +SELGDL+ LPGGYMGKLLIYKSGAVKLRLG+TLYDVSSG +CSFLQH
Sbjct: 241 KVGNLRSSISTASSELGDLRNLPGGYMGKLLIYKSGAVKLRLGDTLYDVSSGLDCSFLQH 300

Query: 301 VVAINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD 334
           VVAINT++G CCDLG++GKRVVVTPDI SLLN++TD
Sbjct: 301 VVAINTDKGQCCDLGEIGKRVVVTPDIGSLLNSMTD 322

[Close]

BLAST of MC10g0227 vs. ExPASy TrEMBL
Match: A0A6J1CHV0 (DNA-directed RNA polymerase III subunit RPC4-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011545 PE=4 SV=1)

HSP 1 Score: 653 bits (1684), Expect = 1.93e-236
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0


Query: 1   MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT 60
           MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT
Sbjct: 1   MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT 60

Query: 61  KVEKKPSVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEE 120
           KVEKKPSVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEE
Sbjct: 61  KVEKKPSVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEE 120

Query: 121 DGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELL 180
           DGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELL
Sbjct: 121 DGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELL 180

Query: 181 DKAEFGLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKV 240
           DKAEFGLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKV
Sbjct: 181 DKAEFGLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKV 240

Query: 241 GSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVV 300
           GSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVV
Sbjct: 241 GSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVV 300

Query: 301 AINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD 334
           AINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD
Sbjct: 301 AINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD 334

[Close]

BLAST of MC10g0227 vs. ExPASy TrEMBL
Match: A0A6J1CGV5 (DNA-directed RNA polymerase III subunit RPC4-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011545 PE=4 SV=1)

HSP 1 Score: 648 bits (1672), Expect = 1.35e-234
Identity = 334/335 (99.70%), Postives = 334/335 (99.70%), Query Frame = 0


Query: 1   MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT 60
           MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT
Sbjct: 1   MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT 60

Query: 61  KVEKKPSVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEE 120
           KVEKKPSVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEE
Sbjct: 61  KVEKKPSVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEE 120

Query: 121 DGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELL 180
           DGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELL
Sbjct: 121 DGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELL 180

Query: 181 DKAEFGLDAPDREYDENAAIPALDLELL-EEKEEKTMYFFQLPACLPLPKQSSTATRKEK 240
           DKAEFGLDAPDREYDENAAIPALDLELL EEKEEKTMYFFQLPACLPLPKQSSTATRKEK
Sbjct: 181 DKAEFGLDAPDREYDENAAIPALDLELLQEEKEEKTMYFFQLPACLPLPKQSSTATRKEK 240

Query: 241 VGSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHV 300
           VGSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHV
Sbjct: 241 VGSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHV 300

Query: 301 VAINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD 334
           VAINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD
Sbjct: 301 VAINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD 335

[Close]

BLAST of MC10g0227 vs. ExPASy TrEMBL
Match: A0A6J1E8A8 (DNA-directed RNA polymerase III subunit rpc4-like OS=Cucurbita moschata OX=3662 GN=LOC111430793 PE=4 SV=1)

HSP 1 Score: 468 bits (1203), Expect = 2.44e-163
Identity = 248/336 (73.81%), Postives = 278/336 (82.74%), Query Frame = 0


Query: 1   MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT 60
           MKNPDPS  PRRK+KFAPK TPR+RPPPAPK EDED   N AQTRYLLRRATENLGKRG 
Sbjct: 1   MKNPDPSP-PRRKIKFAPKSTPRRRPPPAPKTEDEDVVGNVAQTRYLLRRATENLGKRGN 60

Query: 61  KVEKKPSVQVAFGPGAASAS--SSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDV 120
           KVEKK SVQVAFGPGAASAS  SSIRTYGVPK+GNSSRK +IEPE ++DEE ILP P+D 
Sbjct: 61  KVEKKSSVQVAFGPGAASASESSSIRTYGVPKIGNSSRKKDIEPESAEDEELILPPPMDF 120

Query: 121 EEDGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPE 180
           EE G   ++K+ED T             K  KDY+EPWDYH+SYYP TLPLR PYSG PE
Sbjct: 121 EEYGKNLNEKTEDAT-------------KNTKDYKEPWDYHNSYYPTTLPLRMPYSGDPE 180

Query: 181 LLDKAEFGLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKE 240
           +LD+AEFG D  DREYDEN++IPALDL LL+E  E T YFFQLPACLP PK+SST+T KE
Sbjct: 181 MLDEAEFGQDVMDREYDENSSIPALDLGLLDENSENTRYFFQLPACLPFPKRSSTSTGKE 240

Query: 241 KVGSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQH 300
           KVG+ RSS S  +SELGDL+ LPGGYMGKLLIYKSGAVKLRLG+TLYDVSSG +CSFLQH
Sbjct: 241 KVGNLRSSISTASSELGDLRNLPGGYMGKLLIYKSGAVKLRLGDTLYDVSSGLDCSFLQH 300

Query: 301 VVAINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD 334
           VVAINT++G CCDLG++GKRVVVTPDI SLLN++TD
Sbjct: 301 VVAINTDKGQCCDLGEIGKRVVVTPDIGSLLNSMTD 322

[Close]

BLAST of MC10g0227 vs. ExPASy TrEMBL
Match: A0A6J1J8D2 (DNA-directed RNA polymerase III subunit rpc4-like OS=Cucurbita maxima OX=3661 GN=LOC111483488 PE=4 SV=1)

HSP 1 Score: 460 bits (1184), Expect = 1.90e-160
Identity = 247/336 (73.51%), Postives = 277/336 (82.44%), Query Frame = 0


Query: 1   MKNPDPSSRPRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGT 60
           MKNPDPS  PRRK+KFAPK TPR+RPPPAPK EDED + N AQTRYLLRRATENLGKRG 
Sbjct: 1   MKNPDPSP-PRRKIKFAPKSTPRRRPPPAPKTEDEDVDGNVAQTRYLLRRATENLGKRGN 60

Query: 61  KVEKKPSVQVAFGPGAASAS--SSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDV 120
           KVEKK SVQVAFGPGAASAS  SSIRTYGVPK+GNSSRKN+IEPE ++DEE ILPLP+D 
Sbjct: 61  KVEKKSSVQVAFGPGAASASESSSIRTYGVPKIGNSSRKNDIEPESAEDEELILPLPLDF 120

Query: 121 EEDGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPE 180
           EE G   ++K+ED T             K  KDY+EPW+ H+SYYP TLPLR P+SG PE
Sbjct: 121 EEYGKNLNEKTEDAT-------------KNTKDYKEPWNCHNSYYPTTLPLRMPFSGDPE 180

Query: 181 LLDKAEFGLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKE 240
           +LD+AEFG D  DREYDEN+ IPALDL LL+E  E T YFFQLPA LP PK+SST+T KE
Sbjct: 181 MLDEAEFGQDVMDREYDENSNIPALDLGLLDENSENTRYFFQLPARLPFPKRSSTSTGKE 240

Query: 241 KVGSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQH 300
           KVG+ RSS S G+SELGDL+ LPGGYMGKLLIYKSGAVKLRLG+TLYDVSSG +CSFLQH
Sbjct: 241 KVGNLRSSISTGSSELGDLQNLPGGYMGKLLIYKSGAVKLRLGDTLYDVSSGLDCSFLQH 300

Query: 301 VVAINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD 334
           VVAINT++G C DLGD+GKRVVVTPDI SLLN+ TD
Sbjct: 301 VVAINTDKGQCFDLGDIGKRVVVTPDIGSLLNSTTD 322

[Close]

BLAST of MC10g0227 vs. ExPASy TrEMBL
Match: A0A0A0KZ61 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G022870 PE=4 SV=1)

HSP 1 Score: 460 bits (1184), Expect = 2.71e-160
Identity = 247/336 (73.51%), Postives = 275/336 (81.85%), Query Frame = 0


Query: 1   MKNPDPSSRPRRKVKFAPKPTPRKRPPPAP--KPEDEDGNQNAAQTRYLLRRATENLGKR 60
           MKN DPS  PRRKVKFAPK + RKRPPP P  K EDEDG    AQTRYLLRRA ENLGKR
Sbjct: 1   MKNSDPSP-PRRKVKFAPKSSQRKRPPPPPVQKTEDEDGEGYVAQTRYLLRRANENLGKR 60

Query: 61  GTKVEKKPSVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDV 120
             KVEKK SVQVAFGPGA S SSSIRTYGVPKV N SRKN+IEPE+ +DEE +LP+  DV
Sbjct: 61  ANKVEKKSSVQVAFGPGAESTSSSIRTYGVPKVENGSRKNDIEPEVDEDEEFVLPVARDV 120

Query: 121 EEDGMIFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPE 180
            EDG  FD+K++DG  ES+   ++ + TK K+DY+EPWDY +SYYP TLPLR PYSG PE
Sbjct: 121 NEDGKYFDKKTKDGITESS---SSAMETKTKRDYKEPWDYQNSYYPTTLPLRMPYSGDPE 180

Query: 181 LLDKAEFGLDAPDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKE 240
            LD+AEFG D  +REYDEN+ IPALDL LL+E  E T YFFQLPA LPLPKQSSTAT KE
Sbjct: 181 RLDEAEFGQDVMNREYDENSVIPALDLGLLDENTESTKYFFQLPARLPLPKQSSTATGKE 240

Query: 241 KVGSSRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQH 300
           KVG+SRSSNS  +S+L DLKKL  G MGKLLIYKSGA+KLRLG+ LYDVSSGSNCSFLQH
Sbjct: 241 KVGNSRSSNSTSSSDLDDLKKLSAGCMGKLLIYKSGAIKLRLGDILYDVSSGSNCSFLQH 300

Query: 301 VVAINTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD 334
           VVAINTEEG CCDLGD+G RVVVTPDISSLLN+VT+
Sbjct: 301 VVAINTEEGQCCDLGDIGNRVVVTPDISSLLNSVTN 332

[Close]

BLAST of MC10g0227 vs. TAIR 10
Match: AT4G25180.1 (RNA polymerase III RPC4 )

HSP 1 Score: 170.6 bits (431), Expect = 2.1e-42
Identity = 128/332 (38.55%), Postives = 174/332 (52.41%), Query Frame = 0


Query: 7   SSRPRRKVKFAPK-PTPRKRPPPAP--KPEDEDGNQNAAQTRYLLRRATENLGKRGTKVE 66
           S   + K +F P  P P +R P AP    E E+  +N   +R   RR    +G+R     
Sbjct: 3   SGEQKSKRRFQPNPPRPSRRLPIAPTSNTEAEEDEENIKASRQFDRRI---VGRRPKTET 62

Query: 67  KKPSVQVAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGM 126
           K  S +VAF P  +    +IR++GVPK  +    +++ P       SILP    V     
Sbjct: 63  KASSPEVAFQPSLSPL--AIRSFGVPK-EDDKPNSDVNP---SSPASILPAVSSV----- 122

Query: 127 IFDQKSEDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKA 186
                 EDG            +T+   DY EPWDY +SYYP  LPLRKP SG  ELLD+ 
Sbjct: 123 --TAAQEDGEEVHNF------VTRTGDDYVEPWDYRNSYYPTVLPLRKPNSGDIELLDQE 182

Query: 187 EFGLDAPDREYDENAAIPALDLELLE-EKEEKTMYFFQLPACLPLPKQSSTATRKEKVGS 246
           EFG  A +R+YDEN    A +L L   +  +K M+ F++P CLP+ KQ++ AT K  V  
Sbjct: 183 EFGEVAKNRDYDENTINSAEELGLTSVQHSKKQMFIFKIPDCLPVVKQTTGATTKRSVRE 242

Query: 247 SRSSNSKGTSELGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAI 306
             S  S         + LP G+MGK+L+YKSGAVKL++G+ L+DVS G        VVAI
Sbjct: 243 YSSGIS------NPFEGLPEGFMGKMLVYKSGAVKLKVGDALFDVSPGPGTKIPNDVVAI 302

Query: 307 NTEEGHCCDLGDVGKRVVVTPDISSLLNAVTD 335
           + +  +C  +G   K V VTPD+ SLLN  +D
Sbjct: 303 DIKGRNCSRIGSSAKFVTVTPDVESLLNPASD 306

[Close]

BLAST of MC10g0227 vs. TAIR 10
Match: AT5G09380.1 (RNA polymerase III RPC4 )

HSP 1 Score: 167.2 bits (422), Expect = 2.3e-41
Identity = 123/325 (37.85%), Postives = 169/325 (52.00%), Query Frame = 0


Query: 10  PRRKVKFAPKPTPRKRPPPAPKPEDEDGNQNAAQTRYLLRRATENLGKRGTKVEKKPSVQ 69
           P RK+KFAPK  P++ P P  KPE  + N N+AQ   LLRR  E   ++    +K P+ Q
Sbjct: 6   PVRKMKFAPKAPPKRVPKPEVKPEVVEDNSNSAQASELLRRVNERSLRKPKADKKVPASQ 65

Query: 70  VAFGPGAASASSSIRTYGVPKVGNSSRKNNIEPEISDDEESILPLPVDVEEDGMIFDQKS 129
           VA+  G  +++ S +          +R N      S  E                     
Sbjct: 66  VAWLGGVVNSTRSNKYL--------NRSNGAYGSTSTQE--------------------- 125

Query: 130 EDGTPESAMATNAVVLTKAKKDYREPWDYHHSYYPNTLPLRKPYSGHPELLDKAEFGLDA 189
                                +Y+EPWDY +SYYP TLP+R+PY+G PE+LD  EF    
Sbjct: 126 --------------------IEYKEPWDY-YSYYPITLPMRRPYAGDPEVLDVEEF---M 185

Query: 190 PDREYDENAAIPALDLELLEEKEEKTMYFFQLPACLPLPKQSSTATRKEKVGSSRSSNSK 249
               + E++   A +L L+E+  E+ M+F +LP+ +PL   +ST T   +       N K
Sbjct: 186 QAGGHHEDSLNTAANLGLMEDSGEQKMFFMRLPS-VPL---ASTPTENLET----RPNIK 245

Query: 250 GTSE--LGDLKKLPGGYMGKLLIYKSGAVKLRLGETLYDVSSGSNCSFLQHVVAINTEEG 309
           G  E    DLK LP GYMGKLL+YKSGAVK++LGE LYDVS G    F Q V+ +NTE+ 
Sbjct: 246 GPVEKKTVDLKALPEGYMGKLLVYKSGAVKMKLGEVLYDVSPGLKSEFAQDVMVVNTEQK 269

Query: 310 HCCDLGDVGKRVVVTPDISSLLNAV 333
           +CC +GDV K  V+TPDI S+L  +
Sbjct: 306 NCCLVGDVYKHAVLTPDIDSILKDI 269

The following BLAST results are available for this feature:

BLAST of MC10g0227 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P05423	1.1e-08	31.95	DNA-directed RNA polymerase III subunit RPC4 OS=Homo sapiens OX=9606 GN=POLR3D P...	[more]
Q91WD1	3.3e-08	26.59	DNA-directed RNA polymerase III subunit RPC4 OS=Mus musculus OX=10090 GN=Polr3d ...	[more]
Q5E9Z7	1.2e-07	33.79	DNA-directed RNA polymerase III subunit RPC4 OS=Bos taurus OX=9913 GN=POLR3D PE=...	[more]
Q8T1V7	5.2e-06	37.66	DNA-directed RNA polymerase III subunit rpc4 OS=Dictyostelium discoideum OX=4468...	[more]
P25441	4.4e-05	29.01	DNA-directed RNA polymerase III subunit RPC4 OS=Saccharomyces cerevisiae (strain...	[more]

BLAST of MC10g0227 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141051.1	3.98e-236	100.00	DNA-directed RNA polymerase III subunit RPC4-like isoform X2 [Momordica charanti...	[more]
XP_022141050.1	2.79e-234	99.70	DNA-directed RNA polymerase III subunit RPC4-like isoform X1 [Momordica charanti...	[more]
XP_038879339.1	9.57e-170	75.75	DNA-directed RNA polymerase III subunit rpc4-like [Benincasa hispida]	[more]
KAG6576814.1	4.35e-164	74.11	DNA-directed RNA polymerase III subunit RPC4, partial [Cucurbita argyrosperma su...	[more]
XP_022922968.1	5.05e-163	73.81	DNA-directed RNA polymerase III subunit rpc4-like [Cucurbita moschata]	[more]

BLAST of MC10g0227 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CHV0	1.93e-236	100.00	DNA-directed RNA polymerase III subunit RPC4-like isoform X2 OS=Momordica charan...	[more]
A0A6J1CGV5	1.35e-234	99.70	DNA-directed RNA polymerase III subunit RPC4-like isoform X1 OS=Momordica charan...	[more]
A0A6J1E8A8	2.44e-163	73.81	DNA-directed RNA polymerase III subunit rpc4-like OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1J8D2	1.90e-160	73.51	DNA-directed RNA polymerase III subunit rpc4-like OS=Cucurbita maxima OX=3661 GN...	[more]
A0A0A0KZ61	2.71e-160	73.51	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G022870 PE=4 SV=1	[more]

BLAST of MC10g0227 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25180.1	2.1e-42	38.55	RNA polymerase III RPC4	[more]
AT5G09380.1	2.3e-41	37.85	RNA polymerase III RPC4	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007811	DNA-directed RNA polymerase III subunit RPC4	PFAM	PF05132	RNA_pol_Rpc4	coord: 214..325 e-value: 2.1E-26 score: 93.1
IPR007811	DNA-directed RNA polymerase III subunit RPC4	PANTHER	PTHR13408	DNA-DIRECTED RNA POLYMERASE III	coord: 2..334
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..111
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 230..253
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 74..99
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 230..256
None	No IPR available	PANTHER	PTHR13408:SF6	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4-LIKE ISOFORM X1	coord: 2..334

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g0227.1	MC10g0227.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006383	transcription by RNA polymerase III
biological_process	GO:0006412	translation
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005840	ribosome
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0003729	mRNA binding
molecular_function	GO:0003735	structural constituent of ribosome