MC10g0212 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0212
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionmonocopper oxidase-like protein SKS1
LocationMC10: 1540135 .. 1545194 (-)
RNA-Seq ExpressionMC10g0212
SyntenyMC10g0212
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGAGTCCAAGTGAATATAATTATAAAAAGGATGGAAACCGGACGAGACAGTGACTGGTAATTTTTCCTTAAAAAATGAAGTGGATTTTAGGGCAACACTTCCGGTCAGTCTGATTGACCGTATAACCGTTGGGTTTTAATTATTCCATTTTGTTTCTGCAAAAAGCCAAAAGCAGAAGAAGAAGAAGACGAAGAAGAAGAAGTTTCAGAGCTCTCTCTCTCCGATTATGTAAAATCCTTCTCTCATAAATCTCTGAAAACTCTTCGATAATTCTGCTTCCGCTCTTCTAATCTCCAAACTACAGCGACAATGCCTCTACTATGCCGTTCTTCTCTTTCACTGTTTCATCTCTTTCCACTGCTGCTACTGCTTATGCTTCCGTCGCTCTGTTCCGCCGCTGATCCCTACATCTTTTACGAATTTCGCGTCTCTTACATTACTGCCTCTCCCTTGGGCGTTCCTCAACAGGTTCTCTATCTCGCTCTTCTTATGCAACTTTCCGGCTTCCTCTGTTGTGTTCTGTTTCGCTTTTTGCATATTTTCAGATTGAACTGTGCCTTTCTGAATGGTTTCCTCTAGCGTTTGGCTCAAGAGTTTATGTTGTTTGAGGAGTTTTTCTTTTGGAAGTAAGGGTTTAAGGTCTAACTTCCTATTTAGTGATTTTGATTTTTTTCTGCGTGACTCTCAATGAGCGTGAGGAGTTGTTCTGATAATTTTTTGGAGTCTGATTCTGGGAAGTAAAATGGTGAACTTGATTATTATTGATTTTCTCTTGGCTTTAACGCTTTCTGCCGTAAAATTTTGTTTTTGAATCTAGTTATTGGTGTTCTATTGTTTTGCTCTGTAAATCACATAAACAGGAAATGGATGCTGGTGAGAAAAACTGTTTATTTTATTTTGTAGCAATATAGTTTTGTGGATCTTTTTGTTACCTGGTCGCACGGTTTGATGCATTGAACGAATTTAATGCAAATTACAGCCGGATTTAGGGTTTTTTTAATAATACTTTTTCTTTCAGTAGAGTATAAATGTGTTTACTCACAATATTTTATGTTTTGAATCTATTCATAAAATAAACATTTCCAAAATCTCAAGATTCTCGATAAAGTTTTAAAATTAAAAATATATATATTGTTTAAAGATATATATTTAAATAAAAAATTGATAAAAGTATTATATAACGTTTTGAAAATGATGAACTAAGGAATTGTTAGTCAATGCATTTAATTTTAGTAAACATACAGCTAAAGTAGGAACTGGGTATGGAGGCCTTAACATTTAACATCTTTTATCATCAATTTCCCATTTGGTTATGATTTTTCTAAACAATAAAATATCAGTAGATCTGGAGGACTAGAACCTTTCAGCTATATTCGTGTTCTTGTAGTTCATTTTAATGGGTGCTTATTGATCATCTGTCATTTATTTATTTGAATTTTGTATTTATGTTCTGTGGAAGCTTTTCAAGTATTCTTAGGCACTATTTCTTGCTGCAGGTTATAGCCGTGAATGGACAGTTTCCTGGTCCTCGAGTCAATGCTACCACAAATTACAACGTTGCTGTCAATGTGTGGAATGATTTGGATGAGAATCTATTATTGACTTGGTAGGTCTACTTGGAAACAAAGTCTCACTGTTCCATCTCCATTTTCTTTGAGTTGTATTTAAAGGAATGAGATTTTGTAAAGGCTTAAAGTGCTGTGTATCATGGCAGGTCTGGGATTCAAATGCGGCGCAATTCGTGGCAAGATGGTGTTCTTGGCACGAACTGTCCAATTCCTCCAAAGTGGAACTGGACATACCAGTTTCAAGTGAAGGATCAGATTGGAAGTTTCTATTACTTTCCATCTCTCAATTTTCAAAAAGCAGCTGGTGGTTTTGGTCCATTTGTTATTAACAACAGGGAGATTATCCCAATTCCTTTTGCCCAACCTGATGGAGATATTTTCATTATGATTGGTGATTGGTATGCACGCAACCACACGGTTAGTTTCTGAATTGTGGTGTGTAATTATTTCTTTTTATATCAAACAATTTCTTACTGTTTCTTCTTTTTTTTTTTTAATTTTTAATTCAGGCACTCAGGGCAGATCTGGATGCTGGAAAGGATCTTGGAATTCCAGATGGAGTTCTCATGAATGGCAAGGGGCCTTACCAGTACAACAAAACTCTTGTGCCCGCCGGCATACAATATGAAACCATTCAAGTTCATCCAGGTATGGTTCCTCTGTTCGAACTGCTGATGTAATCCAGGTATTATATCAGATCGTTTCTTCCATGGATGATACTCTGCTATTCCTTGAGATCAGTTTAATTTCATCTATTACCAGAAGTATCATGTTTTCTTTTGATCGTTCAGAAAAAGACTATATTTCATCCTGTTGGCATGTTGATGAATGATTAGATAAAAAATAGGGTCACATCTGTTAATGGAATGGGTCCATTGAGAAGTGTAGGATTACACTTACCTCAAACCCACCAATTTGGATTATAAACCCACAAAGTCTTACATTTGTGATTTCTTATCTTAAAAGTTAGAACCCCAGGATAGATGATGCAGAACAGGACGTTCTTTACAACATACTCCCATTTTCGGACATTACTTTGAATAGTTTGGACTCATAACTAATGTTTATCATATGTTTTCAGGCAAAACTTATCGACTTCGAGTGCACAATGTTGGGATCTCTACCAGTTTGAATTTTCGAATCCAGGGTCATAATATGCTTCTGGTAGAAACAGAGGGACATTATACGGTGATGCAAAATTACACAGACTTCGATATTCATGTTGGGCAGTCCTACTCTTTTTTGGTAACCATGGACCAGAATGCCAGTACCGATTACTACATTGTTGCAAGTGCTAGGTTTGTAAACGCATCACTTTGGGAAAAAGTTACTGGTGTTGCCATTTTACACTATTCCAATTCTAAAGGACCAGCAACTGGTCCACTCCCAGATCCTCCAAATGATTTTTATGACAGGGAGAGGTCAATGAACCAGGCCAGGAGTGTAAGGTTCGTTCTTATAGTAGTATCTCTATTTGTATTCTTTATTTCTAGTGATAATGACAGAATTCAAACGTTATCGGTTTTTTTTTTTTCTAGTTATTATGAATATACTGTTAGCTTCAATATTGTTTTGATTCTTTCAATACTGCGGTGCATTATATTATGACCAATCGATATTGGGTCATTTCATCTTCTTGTTATCCAATGATAATTACGGATCAAGATTGGGTTGTTTCAGAGTTCAAACAGTATAAACTTTTTTTCTCCATATTATGAATGTTTTCCATTCACTTGGGAACTGGTAATTCCTTGTGATTAATGTCTATATTTGATGGATTAGGCAAAATGTATCTGCTAGTGGCGCCCGTCCCAATCCGCAGGGATCATTTCATTACGGTCAAATAAATGTCACGCAGACATATTTGTTGAAGGGTGAACCATTGGTGACAATCAACAGAACAGCTCGGGCAACCTTCAATGGTATCTCGTTTGTTCCTCCAAAAACACCAATCAGGCTAGCTGACCAACATAAAGTTAAGGGAGCGTATAAGCTTGATTTTCCAGAGAGGCCATTGAACAGAACTCCACGAGCCGATATATCTATTATAAATGCAACGTATAAAGGCTTTATTGAAGTGATATTTCAGAACAACGATACCATCATCCACAGTATACATATGAATGGCTATTCATTTTTTGTAGCAGGGTGAGATTTTCCTCTCTTTTTCCCACTTATATACATATTTCAGATAAGAAGCTTGCCTCTACTCTATGCATCACTTGCTTTCAAATGTTGATAATTACAAATGTATCATTATTGCAGCATGGGTTATGGTGATTGGTCAGAAGACAAAAGAGGTAGCTACAATAAGTGGGATGCTATTGCTCGATGCACGACGCAGGTTTGTACTTTCAAGTTGTCATGGAAATATTTGTATGAAACGTGCGTGTAATGATGCTTGTCATGAATGCATGGTAAAGTAAATTGAGTTCTTTATTCTTCTCTGACTTTGGCTGTACTTCTTTTCATTCTAAAATTTTAATTGAGCTTAGTAATTCTAGGTTTACTTATAATACAGCTGCAACAATCTAAGACCTGACCGACTATGAAGATCTGCATTTCTCATATTTTCTGTGATGAATTGTGTGAAGTAAGAACTTTGAAGATTTCATTGAAAAGAATGATACTTTCTGGTAGTATTGCATCAGGATCACTGGTGTCAATTGACATCCTTATAAGCTCTGAGATTCTGATTTGGCATTCATTTGATAAATTCTATTATGTTGCGGTGTTCAGGTCTACCCCGGGGCTTGGACAGCAGTTCTTATTTCTCTTGACAATGTTGGATCTTGGAATCTTAGAGCAGAAAATCTAGACCGATGGTATTTAGGTCAAGAAACATATTTGAGAATTGTCAACCCGGAGGAGAATGGGAAAACCGAGATGGCTCCTCCTTCGAACGTTCTATACTGCGGTGCATTGCAGAATTTGCAGAAGTATGTGACTGAACACTTGACATATCTTTTTGTTCTTTCCTTGTGCATCAGACAAGCACAATCTAATCTAATCCTGCCTTGTTTTTTGGTTCTCAGAGAACAGAAACACAACAATGCTGATTCAATCTTCAAGGGACACTCAAAACTCTTCATCTCTCTTCTGATGGCATTCCTTCATATTGTTTTCATTTGTAGCTAAACACACCTGATTGATGAGGCATATTCATTACTTCCCATCAGACACTAGAGTGGTCGTTTTTCGGGCCCATCACTGGTGAGTTGTCGTGTTTCGTTTCGGGTTTGTGTCATTTGTATGTACGCGCATATACACAGCCAGTTAGGTGTGAATGAGTCATTTTCATAGAGAGATTGGTCTAAATGACAATTTTTTCGTGCCCCCTTCTCTTACTCAGTGATTGTAATTTGAATAGTGTTCTAATGCAGCAAAAATTTGCAGTCATGATTCTTGAGAATTCCTTCTCACCATCCTTTTTTGACATAATCTGATATATTTTAATGAAATTCTGTCCCTTATGTGTGAAATGATTGCA

mRNA sequence

AAAGAGTCCAAGTGAATATAATTATAAAAAGGATGGAAACCGGACGAGACAGTGACTGGTAATTTTTCCTTAAAAAATGAAGTGGATTTTAGGGCAACACTTCCGGTCAGTCTGATTGACCGTATAACCGTTGGGTTTTAATTATTCCATTTTGTTTCTGCAAAAAGCCAAAAGCAGAAGAAGAAGAAGACGAAGAAGAAGAAGTTTCAGAGCTCTCTCTCTCCGATTATCGACAATGCCTCTACTATGCCGTTCTTCTCTTTCACTGTTTCATCTCTTTCCACTGCTGCTACTGCTTATGCTTCCGTCGCTCTGTTCCGCCGCTGATCCCTACATCTTTTACGAATTTCGCGTCTCTTACATTACTGCCTCTCCCTTGGGCGTTCCTCAACAGGTTATAGCCGTGAATGGACAGTTTCCTGGTCCTCGAGTCAATGCTACCACAAATTACAACGTTGCTGTCAATGTGTGGAATGATTTGGATGAGAATCTATTATTGACTTGGTCTGGGATTCAAATGCGGCGCAATTCGTGGCAAGATGGTGTTCTTGGCACGAACTGTCCAATTCCTCCAAAGTGGAACTGGACATACCAGTTTCAAGTGAAGGATCAGATTGGAAGTTTCTATTACTTTCCATCTCTCAATTTTCAAAAAGCAGCTGGTGGTTTTGGTCCATTTGTTATTAACAACAGGGAGATTATCCCAATTCCTTTTGCCCAACCTGATGGAGATATTTTCATTATGATTGGTGATTGGTATGCACGCAACCACACGGCACTCAGGGCAGATCTGGATGCTGGAAAGGATCTTGGAATTCCAGATGGAGTTCTCATGAATGGCAAGGGGCCTTACCAGTACAACAAAACTCTTGTGCCCGCCGGCATACAATATGAAACCATTCAAGTTCATCCAGGCAAAACTTATCGACTTCGAGTGCACAATGTTGGGATCTCTACCAGTTTGAATTTTCGAATCCAGGGTCATAATATGCTTCTGGTAGAAACAGAGGGACATTATACGGTGATGCAAAATTACACAGACTTCGATATTCATGTTGGGCAGTCCTACTCTTTTTTGGTAACCATGGACCAGAATGCCAGTACCGATTACTACATTGTTGCAAGTGCTAGGTTTGTAAACGCATCACTTTGGGAAAAAGTTACTGGTGTTGCCATTTTACACTATTCCAATTCTAAAGGACCAGCAACTGGTCCACTCCCAGATCCTCCAAATGATTTTTATGACAGGGAGAGGTCAATGAACCAGGCCAGGAGTGTAAGGCAAAATGTATCTGCTAGTGGCGCCCGTCCCAATCCGCAGGGATCATTTCATTACGGTCAAATAAATGTCACGCAGACATATTTGTTGAAGGGTGAACCATTGGTGACAATCAACAGAACAGCTCGGGCAACCTTCAATGGTATCTCGTTTGTTCCTCCAAAAACACCAATCAGGCTAGCTGACCAACATAAAGTTAAGGGAGCGTATAAGCTTGATTTTCCAGAGAGGCCATTGAACAGAACTCCACGAGCCGATATATCTATTATAAATGCAACGTATAAAGGCTTTATTGAAGTGATATTTCAGAACAACGATACCATCATCCACAGTATACATATGAATGGCTATTCATTTTTTGTAGCAGGCATGGGTTATGGTGATTGGTCAGAAGACAAAAGAGGTAGCTACAATAAGTGGGATGCTATTGCTCGATGCACGACGCAGGTCTACCCCGGGGCTTGGACAGCAGTTCTTATTTCTCTTGACAATGTTGGATCTTGGAATCTTAGAGCAGAAAATCTAGACCGATGGTATTTAGGTCAAGAAACATATTTGAGAATTGTCAACCCGGAGGAGAATGGGAAAACCGAGATGGCTCCTCCTTCGAACGTTCTATACTGCGGTGCATTGCAGAATTTGCAGAAAGAACAGAAACACAACAATGCTGATTCAATCTTCAAGGGACACTCAAAACTCTTCATCTCTCTTCTGATGGCATTCCTTCATATTGTTTTCATTTGTAGCTAAACACACCTGATTGATGAGGCATATTCATTACTTCCCATCAGACACTAGAGTGGTCGTTTTTCGGGCCCATCACTGGTGAGTTGTCGTGTTTCGTTTCGGGTTTGTGTCATTTGTATGTACGCGCATATACACAGCCAGTTAGGTGTGAATGAGTCATTTTCATAGAGAGATTGGTCTAAATGACAATTTTTTCGTGCCCCCTTCTCTTACTCAGTGATTGTAATTTGAATAGTGTTCTAATGCAGCAAAAATTTGCAGTCATGATTCTTGAGAATTCCTTCTCACCATCCTTTTTTGACATAATCTGATATATTTTAATGAAATTCTGTCCCTTATGTGTGAAATGATTGCA

Coding sequence (CDS)

ATGCCTCTACTATGCCGTTCTTCTCTTTCACTGTTTCATCTCTTTCCACTGCTGCTACTGCTTATGCTTCCGTCGCTCTGTTCCGCCGCTGATCCCTACATCTTTTACGAATTTCGCGTCTCTTACATTACTGCCTCTCCCTTGGGCGTTCCTCAACAGGTTATAGCCGTGAATGGACAGTTTCCTGGTCCTCGAGTCAATGCTACCACAAATTACAACGTTGCTGTCAATGTGTGGAATGATTTGGATGAGAATCTATTATTGACTTGGTCTGGGATTCAAATGCGGCGCAATTCGTGGCAAGATGGTGTTCTTGGCACGAACTGTCCAATTCCTCCAAAGTGGAACTGGACATACCAGTTTCAAGTGAAGGATCAGATTGGAAGTTTCTATTACTTTCCATCTCTCAATTTTCAAAAAGCAGCTGGTGGTTTTGGTCCATTTGTTATTAACAACAGGGAGATTATCCCAATTCCTTTTGCCCAACCTGATGGAGATATTTTCATTATGATTGGTGATTGGTATGCACGCAACCACACGGCACTCAGGGCAGATCTGGATGCTGGAAAGGATCTTGGAATTCCAGATGGAGTTCTCATGAATGGCAAGGGGCCTTACCAGTACAACAAAACTCTTGTGCCCGCCGGCATACAATATGAAACCATTCAAGTTCATCCAGGCAAAACTTATCGACTTCGAGTGCACAATGTTGGGATCTCTACCAGTTTGAATTTTCGAATCCAGGGTCATAATATGCTTCTGGTAGAAACAGAGGGACATTATACGGTGATGCAAAATTACACAGACTTCGATATTCATGTTGGGCAGTCCTACTCTTTTTTGGTAACCATGGACCAGAATGCCAGTACCGATTACTACATTGTTGCAAGTGCTAGGTTTGTAAACGCATCACTTTGGGAAAAAGTTACTGGTGTTGCCATTTTACACTATTCCAATTCTAAAGGACCAGCAACTGGTCCACTCCCAGATCCTCCAAATGATTTTTATGACAGGGAGAGGTCAATGAACCAGGCCAGGAGTGTAAGGCAAAATGTATCTGCTAGTGGCGCCCGTCCCAATCCGCAGGGATCATTTCATTACGGTCAAATAAATGTCACGCAGACATATTTGTTGAAGGGTGAACCATTGGTGACAATCAACAGAACAGCTCGGGCAACCTTCAATGGTATCTCGTTTGTTCCTCCAAAAACACCAATCAGGCTAGCTGACCAACATAAAGTTAAGGGAGCGTATAAGCTTGATTTTCCAGAGAGGCCATTGAACAGAACTCCACGAGCCGATATATCTATTATAAATGCAACGTATAAAGGCTTTATTGAAGTGATATTTCAGAACAACGATACCATCATCCACAGTATACATATGAATGGCTATTCATTTTTTGTAGCAGGCATGGGTTATGGTGATTGGTCAGAAGACAAAAGAGGTAGCTACAATAAGTGGGATGCTATTGCTCGATGCACGACGCAGGTCTACCCCGGGGCTTGGACAGCAGTTCTTATTTCTCTTGACAATGTTGGATCTTGGAATCTTAGAGCAGAAAATCTAGACCGATGGTATTTAGGTCAAGAAACATATTTGAGAATTGTCAACCCGGAGGAGAATGGGAAAACCGAGATGGCTCCTCCTTCGAACGTTCTATACTGCGGTGCATTGCAGAATTTGCAGAAAGAACAGAAACACAACAATGCTGATTCAATCTTCAAGGGACACTCAAAACTCTTCATCTCTCTTCTGATGGCATTCCTTCATATTGTTTTCATTTGTAGCTAA

Protein sequence

MPLLCRSSLSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNRTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS
Homology
BLAST of MC10g0212 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 1.5e-258
Identity = 421/578 (72.84%), Postives = 487/578 (84.26%), Query Frame = 0

Query: 17  LLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAV 76
           LL   +L ++  AADP++ Y+FRVSY+TASPLGVPQQVIAVNGQFPGP +NATTNYNV V
Sbjct: 12  LLCFALLSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATTNYNVVV 71

Query: 77  NVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSL 136
           NV+N LDE LLLTW GIQMRRNSWQDGVLGTNCPIPP+WN+TYQFQVKDQIGSF+Y PSL
Sbjct: 72  NVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSFFYSPSL 131

Query: 137 NFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPD 196
           NFQ+A+GGFGP VINNR+IIPIPF QPDG++  +IGDWY ++H ALR  LD+GK+LG+PD
Sbjct: 132 NFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGKELGMPD 191

Query: 197 GVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVE 256
           GVL+NGKGPY+YN + VP GI Y T  V PGKTYR+RVHNVGISTSLNFRIQ H++LLVE
Sbjct: 192 GVLINGKGPYKYNSS-VPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSLLLVE 251

Query: 257 TEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILH 316
           TEGHYT   N+TDFD+HVGQSYSFLVTMDQ+A++DYYIVASARFVN ++W++VTGVAILH
Sbjct: 252 TEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTGVAILH 311

Query: 317 YSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTY 376
           YSNSKGP +GPLP P  D      +M+Q +++RQN SASGARPNPQGSFHYGQIN+T TY
Sbjct: 312 YSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQINITNTY 371

Query: 377 LLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNRTPRADIS 436
           +L+  P   IN   RAT NGISFV P TP+RLAD++KVKGAYKLDFP+RP NR  R D S
Sbjct: 372 ILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKGAYKLDFPDRPFNRPLRLDRS 431

Query: 437 IINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAIARCTT 496
           +INATYKGFI+V+FQNNDT I S H++GYSFFV GM +G WSEDK+GSYN WDAI+R T 
Sbjct: 432 MINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWDAISRSTI 491

Query: 497 QVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPSNVLYC 556
           +VYPG WTAVLISLDNVG WN+R ENLDRWYLG+ETY+RI NPEE+GKTEM PP NVLYC
Sbjct: 492 EVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDPPDNVLYC 551

Query: 557 GALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVF 595
           GAL+NLQKEQ H+ A SI  GH KL   LLM  L  VF
Sbjct: 552 GALKNLQKEQHHSAATSILNGHLKLM--LLMVLLASVF 586

BLAST of MC10g0212 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 858.2 bits (2216), Expect = 5.5e-248
Identity = 397/564 (70.39%), Postives = 470/564 (83.33%), Query Frame = 0

Query: 29  AADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAVNVWNDLDENLLL 88
           A DPY+ Y+F +SYITASPLGVPQQVIAVNG+FPGP +NATTNYNV VNV N LDE LLL
Sbjct: 23  AGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLL 82

Query: 89  TWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNFQKAAGGFGPF 148
           TW G+QMRRNSWQDGVLGTNCPIPP WN+TY FQ+KDQIGS++Y PSLNFQ+A+GGFG  
Sbjct: 83  TWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGAL 142

Query: 149 VINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPDGVLMNGKGPYQY 208
           +INNR+++PIPF +PDG+I  +IGDWY +NHTALR  LD+GK+LG+PDGVL+NGKGP++Y
Sbjct: 143 IINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKY 202

Query: 209 NKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVETEGHYTVMQNYT 268
           N + VP GI++ET+ V PGKTYR+RVHNVGISTSLNFRIQ H +LL+ETEG YT   N+T
Sbjct: 203 NSS-VPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFT 262

Query: 269 DFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILHYSNSKGPATGPL 328
           DFD+HVGQSYSFLVTMDQNA++DYYIVASARFVN ++W++VTGV ILHYSNSKGPA+GPL
Sbjct: 263 DFDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPL 322

Query: 329 PDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTYLLKGEPLVTINR 388
           P    D      +MNQ R+++QN SASGARPNPQGSFHYGQIN+T+TY+L+  P   IN 
Sbjct: 323 PVSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPTKING 382

Query: 389 TARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN-RTPRADISIINATYKGFIE 448
             RAT NGISFV P TP+RLAD HKVKG Y LDFP+RPL+ + PR   SIINATYKGFI+
Sbjct: 383 KLRATLNGISFVNPSTPMRLADDHKVKGDYMLDFPDRPLDEKLPRLSSSIINATYKGFIQ 442

Query: 449 VIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAIARCTTQVYPGAWTAVL 508
           VIFQNNDT I S H++GY+F+V  M +G WSED+  SYN WDA+AR T +VYPGAWTAVL
Sbjct: 443 VIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAWTAVL 502

Query: 509 ISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPSNVLYCGALQNLQKEQK 568
           ISLDNVG WN+R ENLDRWYLGQETY+RI+NPEENG TEM PP NV+YCGALQ +QKEQ 
Sbjct: 503 ISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCGALQAMQKEQH 562

Query: 569 HNNA-DSIFKGHSKLFISLLMAFL 591
           H++A  S+  G   L  S++M  L
Sbjct: 563 HSSATKSMTNGQLILIFSMMMVLL 585

BLAST of MC10g0212 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 4.1e-243
Identity = 406/584 (69.52%), Postives = 465/584 (79.62%), Query Frame = 0

Query: 14  LFPLLLLLMLP--SLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTN 73
           LF +LLL+     S C AADPY FY F VSYITASPLGVPQQVIA+NG+FPGP +N TTN
Sbjct: 3   LFKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTN 62

Query: 74  YNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFY 133
            N+ VNV N LDE LLL W+GIQ RR SWQDGVLGTNCPIPPKWNWTY+FQVKDQIGSF+
Sbjct: 63  ENLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFF 122

Query: 134 YFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKD 193
           YFPSL+FQ+A+GGFG FV+N R IIP+PF+ PDGDI + IGDWY RNHTALR  LD GKD
Sbjct: 123 YFPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKD 182

Query: 194 LGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHN 253
           LG+PDGVL+NGKGPY+YN TLV  GI +ETI VHPGKTYRLRV NVGISTSLNFRIQGHN
Sbjct: 183 LGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHN 242

Query: 254 MLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTG 313
           ++L E+EG YTV QNYT  DIHVGQSYSFLVTMDQNAS+DYYIVASAR VN ++W +VTG
Sbjct: 243 LVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTG 302

Query: 314 VAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQIN 373
           V IL Y+NSKG A G LP  P D +D+  SMNQARS+R NVSASGARPNPQGSF YG IN
Sbjct: 303 VGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSIN 362

Query: 374 VTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNRTP 433
           VT  Y+L+  P VTI+   R T NGISF  P TPIRLAD+ KVK  YKLDFP+RPL    
Sbjct: 363 VTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKDVYKLDFPKRPLTGPA 422

Query: 434 RADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAI 493
           +   SIIN TY+GF+EV+ QNNDT + S HM+GY+FFV GM YG+W+E+ RG+YNKWD I
Sbjct: 423 KVATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGI 482

Query: 494 ARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPS 553
           AR T QVYPGAW+A+LISLDN G+WNLR ENLD WYLGQETY+R+VNP+EN KTE   P 
Sbjct: 483 ARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPD 542

Query: 554 NVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFI 596
           NVLYCGAL  LQK QK +++ S   G    F SL M  + +V +
Sbjct: 543 NVLYCGALSKLQKPQKVSSSASKSIG----FTSLSMVVMALVMM 582

BLAST of MC10g0212 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 1.3e-140
Identity = 242/541 (44.73%), Postives = 349/541 (64.51%), Query Frame = 0

Query: 29  AADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAVNVWNDLDENLLL 88
           A DPY  + + V+Y TASPLGVPQQVI +NGQFPGP +N+T+N NV +NV+N+LDE  LL
Sbjct: 23  AEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNNNVIINVFNNLDEPFLL 82

Query: 89  TWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNFQKAAGGFGPF 148
           TW+GIQ R+N WQDG  GT CPI P  N+TY FQ KDQIGS++Y+P+    +AAGG+G  
Sbjct: 83  TWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFYYPTTGMHRAAGGYGGL 142

Query: 149 VINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPDGVLMNGKGPYQY 208
            +N+R +IP+P+A P+ D  ++IGDWY ++HT L+  LD G+ +G PDG+++NGK     
Sbjct: 143 RVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDGGRTIGRPDGIVINGKSG--- 202

Query: 209 NKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVETEGHYTVMQNYT 268
                  G       + PGKTYR+R+ NVG+ TS+NFRIQ H M LVE EG + +  +Y 
Sbjct: 203 ----KGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEMEGSHVLQNDYD 262

Query: 269 DFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILHYSNSKGPATGPL 328
             D+HVGQ +  +VT +Q    DYY+VAS+RF+       +T   +L Y   KGPA+  L
Sbjct: 263 SLDVHVGQCFGTIVTANQEPK-DYYMVASSRFLKT----VITTTGLLRYEGGKGPASSQL 322

Query: 329 PDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTYLLKGEPLVTINR 388
           P  P  +     S+NQ RS R N++AS ARPNPQGS+HYG+IN+T+T  L       ++ 
Sbjct: 323 PAGPVGW---AWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQ-GKVDG 382

Query: 389 TARATFNGISFVPPKTPIRLADQHKVKG-AYKLDF----PERPLNRTPRADISIINATYK 448
             R   NG+S   P+TP++LA+   +    +K D     P     +  + + +++N T++
Sbjct: 383 KLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLNITHR 442

Query: 449 GFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAIARCTTQVYPGAW 508
            F+EV+F+N++  + S H++GYSFF   +  G W+ +KR +YN  DA++R T QVYP  W
Sbjct: 443 TFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCW 502

Query: 509 TAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPSNVLYCGALQNLQ 565
            A+L++ DN G WN+R+EN +R YLGQ+ Y  +++PE++ + E   P   L CG ++N  
Sbjct: 503 AAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKNTP 547

BLAST of MC10g0212 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 4.8e-135
Identity = 245/552 (44.38%), Postives = 345/552 (62.50%), Query Frame = 0

Query: 19  LLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAVNV 78
           LLL L     A DPY+++ + V+Y T +PLGVPQQ I +NGQFPGPR+N T+N N+ VNV
Sbjct: 11  LLLCLSVGVIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNIVVNV 70

Query: 79  WNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNF 138
           +N+LDE  L TW+G+Q R+NSWQDG  GT CPI P  N+TY+FQVKDQIGS+ YFP+   
Sbjct: 71  FNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFPTTAL 130

Query: 139 QKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPDGV 198
            +AAGG+G   +++R +IP+PF  P  +  + +GDWY + H  L+  LD G+ +G PDG+
Sbjct: 131 HRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGRPDGI 190

Query: 199 LMNGKGPYQYNKTLVPAGIQYETI-QVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVET 258
           ++NGK            G   E +  +  GKTYR R  N+G+ +S+N R QGH M LVE 
Sbjct: 191 IINGKS--------AKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVEL 250

Query: 259 EGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILHY 318
           EG +TV   Y   D+HVGQ  S LVT DQ    DYY+V S+RF+  +L    + VAI+ Y
Sbjct: 251 EGSHTVQNIYDSLDLHVGQCLSVLVTADQEPK-DYYLVVSSRFLKQAL----SSVAIIRY 310

Query: 319 SNSKGPATGPLPDPPNDFYDR-ERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTY 378
           +N KGPA+  LP PP +  +    SMNQ RS R N++AS ARPNPQGS+HYGQIN+T+T 
Sbjct: 311 ANGKGPASPELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTI 370

Query: 379 LLKGEPLVTINRTARATFNGISFVPPKTPIRLADQH-KVKGAYKLDF-----PERPLNRT 438
            +    +  +    R   NGIS    +TP++L +       A+K D      P  P   T
Sbjct: 371 KIFNS-MSQVGGKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPADPSKLT 430

Query: 439 PRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDA 498
              ++   NATY+ F+E+IF+N++  I + H++GYSFF   +  G WS +KR +YN  D 
Sbjct: 431 IATNVK--NATYRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDG 490

Query: 499 IARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPP 558
           ++R   QVYP +W A++++ DN G WNLR+E  ++ YLG++ Y  +++P  + + E   P
Sbjct: 491 LSRNNIQVYPNSWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIP 546

Query: 559 SNVLYCGALQNL 563
            N   CG ++ L
Sbjct: 551 DNHPLCGIVKGL 546

BLAST of MC10g0212 vs. NCBI nr
Match: XP_022140913.1 (monocopper oxidase-like protein SKS1 [Momordica charantia])

HSP 1 Score: 1229 bits (3181), Expect = 0.0
Identity = 597/597 (100.00%), Postives = 597/597 (100.00%), Query Frame = 0

Query: 1   MPLLCRSSLSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQ 60
           MPLLCRSSLSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQ
Sbjct: 1   MPLLCRSSLSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQ 60

Query: 61  FPGPRVNATTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQ 120
           FPGPRVNATTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQ
Sbjct: 61  FPGPRVNATTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQ 120

Query: 121 FQVKDQIGSFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHT 180
           FQVKDQIGSFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHT
Sbjct: 121 FQVKDQIGSFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHT 180

Query: 181 ALRADLDAGKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGIS 240
           ALRADLDAGKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGIS
Sbjct: 181 ALRADLDAGKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGIS 240

Query: 241 TSLNFRIQGHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARF 300
           TSLNFRIQGHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARF
Sbjct: 241 TSLNFRIQGHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARF 300

Query: 301 VNASLWEKVTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPN 360
           VNASLWEKVTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPN
Sbjct: 301 VNASLWEKVTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPN 360

Query: 361 PQGSFHYGQINVTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKL 420
           PQGSFHYGQINVTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKL
Sbjct: 361 PQGSFHYGQINVTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKL 420

Query: 421 DFPERPLNRTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSED 480
           DFPERPLNRTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSED
Sbjct: 421 DFPERPLNRTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSED 480

Query: 481 KRGSYNKWDAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPE 540
           KRGSYNKWDAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPE
Sbjct: 481 KRGSYNKWDAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPE 540

Query: 541 ENGKTEMAPPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS 597
           ENGKTEMAPPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS
Sbjct: 541 ENGKTEMAPPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS 597

BLAST of MC10g0212 vs. NCBI nr
Match: XP_038880777.1 (monocopper oxidase-like protein SKS1 [Benincasa hispida])

HSP 1 Score: 1116 bits (2886), Expect = 0.0
Identity = 538/589 (91.34%), Postives = 563/589 (95.59%), Query Frame = 0

Query: 10  SLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNAT 69
           SLF+LFP++ L   PSL SAADPY+FY+FRVSYITASPLGVPQQVIAVN QFPGP +NAT
Sbjct: 5   SLFYLFPIIFL---PSLSSAADPYVFYDFRVSYITASPLGVPQQVIAVNEQFPGPPINAT 64

Query: 70  TNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGS 129
           TNYNVAVNVWNDLDENLL+TWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGS
Sbjct: 65  TNYNVAVNVWNDLDENLLITWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGS 124

Query: 130 FYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAG 189
           FYYFPSLNFQKA+GGFGP VINNREIIPIPF QPDGDI IMIGDWY RNHTALR DLDAG
Sbjct: 125 FYYFPSLNFQKASGGFGPIVINNREIIPIPFPQPDGDISIMIGDWYIRNHTALRIDLDAG 184

Query: 190 KDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQG 249
           K+LGIPDGVL+NGKGPYQYN TLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQ 
Sbjct: 185 KELGIPDGVLINGKGPYQYNTTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQS 244

Query: 250 HNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKV 309
           HNMLL ETEGHYTVMQNY+DFDIHVGQSYSFLVTMDQNASTDYYIVASARFVN SLWEKV
Sbjct: 245 HNMLLAETEGHYTVMQNYSDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNQSLWEKV 304

Query: 310 TGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQ 369
           TGVAILHYSNSKGPATGPLPDPPNDFYD+ERSMNQARSVRQNVSASGARPNPQGSFHYGQ
Sbjct: 305 TGVAILHYSNSKGPATGPLPDPPNDFYDKERSMNQARSVRQNVSASGARPNPQGSFHYGQ 364

Query: 370 INVTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNR 429
           INVTQTYLLKGEPLVTIN+T RATFNGISF+PP+TPIRLADQHKVKGAYKLDFP+RPLNR
Sbjct: 365 INVTQTYLLKGEPLVTINKTTRATFNGISFIPPQTPIRLADQHKVKGAYKLDFPDRPLNR 424

Query: 430 TPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWD 489
           TPRADISIINATYKGFIEVIFQNND+IIHSIHM+GYSFFV GMGYGDWSEDKRGSYNKWD
Sbjct: 425 TPRADISIINATYKGFIEVIFQNNDSIIHSIHMDGYSFFVVGMGYGDWSEDKRGSYNKWD 484

Query: 490 AIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAP 549
           AI RCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRI+NPEENGKTEMA 
Sbjct: 485 AITRCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIINPEENGKTEMAA 544

Query: 550 PSNVLYCGALQNLQKEQKHN-NADSIFKGHSKLFISLLMAFLHIVFICS 597
           PSNVLYCGAL++LQKEQ H+ NA SI KGHSKLFI+LLMA L++VFI S
Sbjct: 545 PSNVLYCGALRSLQKEQHHHSNAKSILKGHSKLFITLLMALLNLVFIFS 590

BLAST of MC10g0212 vs. NCBI nr
Match: XP_008439091.1 (PREDICTED: monocopper oxidase-like protein SKS1 [Cucumis melo])

HSP 1 Score: 1107 bits (2863), Expect = 0.0
Identity = 535/591 (90.52%), Postives = 562/591 (95.09%), Query Frame = 0

Query: 10  SLFHL-FPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNA 69
           SLFHL FP++ L   PSLCSAADPY+FY+FR+SYITASPLGVPQQVIAVN QFPGP +NA
Sbjct: 5   SLFHLIFPIIFL---PSLCSAADPYVFYDFRLSYITASPLGVPQQVIAVNEQFPGPPINA 64

Query: 70  TTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 129
           TTNYNV VNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG
Sbjct: 65  TTNYNVVVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 124

Query: 130 SFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDA 189
           SFYYFPSLNFQ+A+GGFGP VINNREIIPIPF +PDG+I IMIGDWY RNH ALRADLDA
Sbjct: 125 SFYYFPSLNFQRASGGFGPIVINNREIIPIPFPEPDGEISIMIGDWYTRNHKALRADLDA 184

Query: 190 GKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQ 249
           GK+LGIPDGVL+NGKGPYQYN TLVPAGIQYETIQVHPGKTYR+RVHNVGISTSLNFRIQ
Sbjct: 185 GKELGIPDGVLINGKGPYQYNTTLVPAGIQYETIQVHPGKTYRVRVHNVGISTSLNFRIQ 244

Query: 250 GHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEK 309
            H MLL ETEGHYTVMQN+TDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVN SLW+K
Sbjct: 245 SHYMLLAETEGHYTVMQNFTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNESLWQK 304

Query: 310 VTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYG 369
           VTGVAILHYSNSKGPATGPLPDPPNDFYD+ERSMNQARSVRQNVSASGARPNPQGSFHYG
Sbjct: 305 VTGVAILHYSNSKGPATGPLPDPPNDFYDKERSMNQARSVRQNVSASGARPNPQGSFHYG 364

Query: 370 QINVTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN 429
           QINVT+TYLLK EPLVTIN+TARATFNGISFVPPKTPIRLADQHKVKGAYKLDFP+RPLN
Sbjct: 365 QINVTRTYLLKSEPLVTINKTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPDRPLN 424

Query: 430 RTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKW 489
           RTP+ADISIINATYKGFIEVIFQNND+IIHSIHM+GYSFFV GMGYGDWSEDKRGSYNKW
Sbjct: 425 RTPKADISIINATYKGFIEVIFQNNDSIIHSIHMDGYSFFVVGMGYGDWSEDKRGSYNKW 484

Query: 490 DAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMA 549
           DAI RCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRI+NPEENGKTEMA
Sbjct: 485 DAITRCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIINPEENGKTEMA 544

Query: 550 PPSNVLYCGALQNLQKEQKH--NNADSIFKGHSKLFISLLMAFLHIVFICS 597
            PSNVLYCGALQ+LQKEQ H  NNA SIFKGHSKLFI+LLMA L++V I S
Sbjct: 545 APSNVLYCGALQSLQKEQHHKSNNAKSIFKGHSKLFIALLMALLNLVSIFS 592

BLAST of MC10g0212 vs. NCBI nr
Match: KAG6576799.1 (Monocopper oxidase-like protein SKS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1101 bits (2848), Expect = 0.0
Identity = 526/592 (88.85%), Postives = 560/592 (94.59%), Query Frame = 0

Query: 6   RSSLSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPR 65
           R  LSLF+L P++ L   PS CSAADPY  YEFRVSYITASPLGVPQ+VIAVNG FPGP 
Sbjct: 5   RPVLSLFYLLPIIFL---PSFCSAADPYASYEFRVSYITASPLGVPQKVIAVNGNFPGPP 64

Query: 66  VNATTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKD 125
           +N TTNYNVAVNVWNDLDENLL+TWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKD
Sbjct: 65  INVTTNYNVAVNVWNDLDENLLMTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKD 124

Query: 126 QIGSFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRAD 185
           QIGSF+YFPS+NFQKA+GGFGPFVINNR+IIPIPFAQP+GDIFIMIGDW+ R+HTALRAD
Sbjct: 125 QIGSFFYFPSINFQKASGGFGPFVINNRDIIPIPFAQPEGDIFIMIGDWFTRDHTALRAD 184

Query: 186 LDAGKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNF 245
           L +GK+LGIPDGVL+NGKGPYQYN TLVPAGIQYETI+V PGKTYRLRVHNVGISTSLNF
Sbjct: 185 LSSGKELGIPDGVLINGKGPYQYNATLVPAGIQYETIRVDPGKTYRLRVHNVGISTSLNF 244

Query: 246 RIQGHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASL 305
           RIQ HNMLL ETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVN SL
Sbjct: 245 RIQSHNMLLAETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNESL 304

Query: 306 WEKVTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSF 365
           W+KVTGVAILHYSNSKGPATGPLPDPPNDFYD+ERSMNQARSVRQNVSASGARPNPQGSF
Sbjct: 305 WQKVTGVAILHYSNSKGPATGPLPDPPNDFYDKERSMNQARSVRQNVSASGARPNPQGSF 364

Query: 366 HYGQINVTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPER 425
           HYGQINVTQTYLLKGEPLVTIN+TARATFNGISF+PPKTPIRLADQHKVKGAYKLDFP+ 
Sbjct: 365 HYGQINVTQTYLLKGEPLVTINKTARATFNGISFIPPKTPIRLADQHKVKGAYKLDFPDM 424

Query: 426 PLNRTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSY 485
           PLNRTPRADISII+A YKGFIEVIFQNND+IIHS+H++GYSFFV GMGYGDWSEDKRGSY
Sbjct: 425 PLNRTPRADISIIDAAYKGFIEVIFQNNDSIIHSVHLDGYSFFVVGMGYGDWSEDKRGSY 484

Query: 486 NKWDAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKT 545
           NKWDAI R T+QVYPGAWTAVLISLDNVG WNLRAENLDRWYLGQETYLRIVNPEENGKT
Sbjct: 485 NKWDAITRSTSQVYPGAWTAVLISLDNVGVWNLRAENLDRWYLGQETYLRIVNPEENGKT 544

Query: 546 EMAPPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS 597
           EMA PSNVLYCGALQ+LQKEQ H NA+SIFKGHSKLFI+LLMA L++ F+ S
Sbjct: 545 EMAAPSNVLYCGALQSLQKEQHHGNANSIFKGHSKLFITLLMALLNVAFLLS 593

BLAST of MC10g0212 vs. NCBI nr
Match: XP_011651117.1 (monocopper oxidase-like protein SKS1 [Cucumis sativus] >KGN57269.1 hypothetical protein Csa_010920 [Cucumis sativus])

HSP 1 Score: 1097 bits (2837), Expect = 0.0
Identity = 526/579 (90.85%), Postives = 550/579 (94.99%), Query Frame = 0

Query: 20  LLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAVNVW 79
           ++ LPSLCSAADPY+ Y+FR+SYITASPLGVPQQVIAVN QFPGP +NATTNYNV VNVW
Sbjct: 14  IIFLPSLCSAADPYVSYDFRLSYITASPLGVPQQVIAVNEQFPGPPINATTNYNVVVNVW 73

Query: 80  NDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNFQ 139
           NDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNFQ
Sbjct: 74  NDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNFQ 133

Query: 140 KAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPDGVL 199
           +A+GGFGP VINNREII IPF QPDG+I IMIGDWY RNHTALRADLDAGK+LGIPDGVL
Sbjct: 134 RASGGFGPIVINNREIISIPFPQPDGEISIMIGDWYTRNHTALRADLDAGKELGIPDGVL 193

Query: 200 MNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVETEG 259
           +NGKGPYQYN TLVPAGIQYETIQVHPGKTYR+RVHNVGISTSLNFRIQ HNMLL ETEG
Sbjct: 194 INGKGPYQYNTTLVPAGIQYETIQVHPGKTYRVRVHNVGISTSLNFRIQSHNMLLAETEG 253

Query: 260 HYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILHYSN 319
           HYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVN SLW+KVTGVAILHYSN
Sbjct: 254 HYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNESLWQKVTGVAILHYSN 313

Query: 320 SKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTYLLK 379
           SKGPATGPLPDPPNDFYD+ERSMNQARSVRQNVSASGARPNPQGSFHYGQINVT TYLLK
Sbjct: 314 SKGPATGPLPDPPNDFYDKERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTHTYLLK 373

Query: 380 GEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNRTPRADISIIN 439
            EPLVTIN+TARATFNGISFVPPKTPIRLADQHKVKGAYKLDFP  PLNRTP+ADISIIN
Sbjct: 374 SEPLVTINKTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPHTPLNRTPKADISIIN 433

Query: 440 ATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAIARCTTQVY 499
           ATYKGFIEVIFQNND+IIHSIHM+GYSFFV GMGYGDWSEDKRGSYNKWDAI RCTTQVY
Sbjct: 434 ATYKGFIEVIFQNNDSIIHSIHMDGYSFFVVGMGYGDWSEDKRGSYNKWDAITRCTTQVY 493

Query: 500 PGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPSNVLYCGAL 559
           PGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRI+NPEENGKTEM  PSNVLYCGAL
Sbjct: 494 PGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIINPEENGKTEMEAPSNVLYCGAL 553

Query: 560 QNLQKEQKH-NNADSIFKGHSKLFISLLMAFLHIVFICS 597
           Q+LQKEQ H +N  SIFKGHSKLFI+LLMA L++V I S
Sbjct: 554 QSLQKEQHHKSNGKSIFKGHSKLFIALLMALLNLVSIFS 592

BLAST of MC10g0212 vs. ExPASy TrEMBL
Match: A0A6J1CJ41 (monocopper oxidase-like protein SKS1 OS=Momordica charantia OX=3673 GN=LOC111011462 PE=3 SV=1)

HSP 1 Score: 1229 bits (3181), Expect = 0.0
Identity = 597/597 (100.00%), Postives = 597/597 (100.00%), Query Frame = 0

Query: 1   MPLLCRSSLSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQ 60
           MPLLCRSSLSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQ
Sbjct: 1   MPLLCRSSLSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQ 60

Query: 61  FPGPRVNATTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQ 120
           FPGPRVNATTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQ
Sbjct: 61  FPGPRVNATTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQ 120

Query: 121 FQVKDQIGSFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHT 180
           FQVKDQIGSFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHT
Sbjct: 121 FQVKDQIGSFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHT 180

Query: 181 ALRADLDAGKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGIS 240
           ALRADLDAGKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGIS
Sbjct: 181 ALRADLDAGKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGIS 240

Query: 241 TSLNFRIQGHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARF 300
           TSLNFRIQGHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARF
Sbjct: 241 TSLNFRIQGHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARF 300

Query: 301 VNASLWEKVTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPN 360
           VNASLWEKVTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPN
Sbjct: 301 VNASLWEKVTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPN 360

Query: 361 PQGSFHYGQINVTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKL 420
           PQGSFHYGQINVTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKL
Sbjct: 361 PQGSFHYGQINVTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKL 420

Query: 421 DFPERPLNRTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSED 480
           DFPERPLNRTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSED
Sbjct: 421 DFPERPLNRTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSED 480

Query: 481 KRGSYNKWDAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPE 540
           KRGSYNKWDAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPE
Sbjct: 481 KRGSYNKWDAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPE 540

Query: 541 ENGKTEMAPPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS 597
           ENGKTEMAPPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS
Sbjct: 541 ENGKTEMAPPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS 597

BLAST of MC10g0212 vs. ExPASy TrEMBL
Match: A0A1S3AXI5 (monocopper oxidase-like protein SKS1 OS=Cucumis melo OX=3656 GN=LOC103483983 PE=3 SV=1)

HSP 1 Score: 1107 bits (2863), Expect = 0.0
Identity = 535/591 (90.52%), Postives = 562/591 (95.09%), Query Frame = 0

Query: 10  SLFHL-FPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNA 69
           SLFHL FP++ L   PSLCSAADPY+FY+FR+SYITASPLGVPQQVIAVN QFPGP +NA
Sbjct: 5   SLFHLIFPIIFL---PSLCSAADPYVFYDFRLSYITASPLGVPQQVIAVNEQFPGPPINA 64

Query: 70  TTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 129
           TTNYNV VNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG
Sbjct: 65  TTNYNVVVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIG 124

Query: 130 SFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDA 189
           SFYYFPSLNFQ+A+GGFGP VINNREIIPIPF +PDG+I IMIGDWY RNH ALRADLDA
Sbjct: 125 SFYYFPSLNFQRASGGFGPIVINNREIIPIPFPEPDGEISIMIGDWYTRNHKALRADLDA 184

Query: 190 GKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQ 249
           GK+LGIPDGVL+NGKGPYQYN TLVPAGIQYETIQVHPGKTYR+RVHNVGISTSLNFRIQ
Sbjct: 185 GKELGIPDGVLINGKGPYQYNTTLVPAGIQYETIQVHPGKTYRVRVHNVGISTSLNFRIQ 244

Query: 250 GHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEK 309
            H MLL ETEGHYTVMQN+TDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVN SLW+K
Sbjct: 245 SHYMLLAETEGHYTVMQNFTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNESLWQK 304

Query: 310 VTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYG 369
           VTGVAILHYSNSKGPATGPLPDPPNDFYD+ERSMNQARSVRQNVSASGARPNPQGSFHYG
Sbjct: 305 VTGVAILHYSNSKGPATGPLPDPPNDFYDKERSMNQARSVRQNVSASGARPNPQGSFHYG 364

Query: 370 QINVTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN 429
           QINVT+TYLLK EPLVTIN+TARATFNGISFVPPKTPIRLADQHKVKGAYKLDFP+RPLN
Sbjct: 365 QINVTRTYLLKSEPLVTINKTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPDRPLN 424

Query: 430 RTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKW 489
           RTP+ADISIINATYKGFIEVIFQNND+IIHSIHM+GYSFFV GMGYGDWSEDKRGSYNKW
Sbjct: 425 RTPKADISIINATYKGFIEVIFQNNDSIIHSIHMDGYSFFVVGMGYGDWSEDKRGSYNKW 484

Query: 490 DAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMA 549
           DAI RCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRI+NPEENGKTEMA
Sbjct: 485 DAITRCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIINPEENGKTEMA 544

Query: 550 PPSNVLYCGALQNLQKEQKH--NNADSIFKGHSKLFISLLMAFLHIVFICS 597
            PSNVLYCGALQ+LQKEQ H  NNA SIFKGHSKLFI+LLMA L++V I S
Sbjct: 545 APSNVLYCGALQSLQKEQHHKSNNAKSIFKGHSKLFIALLMALLNLVSIFS 592

BLAST of MC10g0212 vs. ExPASy TrEMBL
Match: A0A0A0L8C4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G175700 PE=3 SV=1)

HSP 1 Score: 1097 bits (2837), Expect = 0.0
Identity = 526/579 (90.85%), Postives = 550/579 (94.99%), Query Frame = 0

Query: 20  LLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAVNVW 79
           ++ LPSLCSAADPY+ Y+FR+SYITASPLGVPQQVIAVN QFPGP +NATTNYNV VNVW
Sbjct: 14  IIFLPSLCSAADPYVSYDFRLSYITASPLGVPQQVIAVNEQFPGPPINATTNYNVVVNVW 73

Query: 80  NDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNFQ 139
           NDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNFQ
Sbjct: 74  NDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNFQ 133

Query: 140 KAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPDGVL 199
           +A+GGFGP VINNREII IPF QPDG+I IMIGDWY RNHTALRADLDAGK+LGIPDGVL
Sbjct: 134 RASGGFGPIVINNREIISIPFPQPDGEISIMIGDWYTRNHTALRADLDAGKELGIPDGVL 193

Query: 200 MNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVETEG 259
           +NGKGPYQYN TLVPAGIQYETIQVHPGKTYR+RVHNVGISTSLNFRIQ HNMLL ETEG
Sbjct: 194 INGKGPYQYNTTLVPAGIQYETIQVHPGKTYRVRVHNVGISTSLNFRIQSHNMLLAETEG 253

Query: 260 HYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILHYSN 319
           HYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVN SLW+KVTGVAILHYSN
Sbjct: 254 HYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNESLWQKVTGVAILHYSN 313

Query: 320 SKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTYLLK 379
           SKGPATGPLPDPPNDFYD+ERSMNQARSVRQNVSASGARPNPQGSFHYGQINVT TYLLK
Sbjct: 314 SKGPATGPLPDPPNDFYDKERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTHTYLLK 373

Query: 380 GEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNRTPRADISIIN 439
            EPLVTIN+TARATFNGISFVPPKTPIRLADQHKVKGAYKLDFP  PLNRTP+ADISIIN
Sbjct: 374 SEPLVTINKTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPHTPLNRTPKADISIIN 433

Query: 440 ATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAIARCTTQVY 499
           ATYKGFIEVIFQNND+IIHSIHM+GYSFFV GMGYGDWSEDKRGSYNKWDAI RCTTQVY
Sbjct: 434 ATYKGFIEVIFQNNDSIIHSIHMDGYSFFVVGMGYGDWSEDKRGSYNKWDAITRCTTQVY 493

Query: 500 PGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPSNVLYCGAL 559
           PGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRI+NPEENGKTEM  PSNVLYCGAL
Sbjct: 494 PGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIINPEENGKTEMEAPSNVLYCGAL 553

Query: 560 QNLQKEQKH-NNADSIFKGHSKLFISLLMAFLHIVFICS 597
           Q+LQKEQ H +N  SIFKGHSKLFI+LLMA L++V I S
Sbjct: 554 QSLQKEQHHKSNGKSIFKGHSKLFIALLMALLNLVSIFS 592

BLAST of MC10g0212 vs. ExPASy TrEMBL
Match: A0A6J1E5V7 (monocopper oxidase-like protein SKS1 OS=Cucurbita moschata OX=3662 GN=LOC111430865 PE=3 SV=1)

HSP 1 Score: 1096 bits (2834), Expect = 0.0
Identity = 524/592 (88.51%), Postives = 558/592 (94.26%), Query Frame = 0

Query: 6   RSSLSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPR 65
           R  LSLF+L P++ L   PS CSAADPY  YEFRVSYITASPLGVPQ+VIAVNG FPGP 
Sbjct: 5   RPVLSLFYLLPIIFL---PSFCSAADPYASYEFRVSYITASPLGVPQKVIAVNGNFPGPP 64

Query: 66  VNATTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKD 125
           +N TTNYNVAVNVWNDLDENLL+TWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKD
Sbjct: 65  INVTTNYNVAVNVWNDLDENLLMTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKD 124

Query: 126 QIGSFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRAD 185
           QIGSF+YFPS+NFQKA+GGFGPFVINNR+IIPIPFAQP+GDIFIMIGDW+ R+HTALRAD
Sbjct: 125 QIGSFFYFPSINFQKASGGFGPFVINNRDIIPIPFAQPEGDIFIMIGDWFTRDHTALRAD 184

Query: 186 LDAGKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNF 245
           L +GK+LGIPDGVL+NGKGPYQYN TLVPAGIQYETI+V PGKTYRLRVHNVGISTSLNF
Sbjct: 185 LSSGKELGIPDGVLINGKGPYQYNATLVPAGIQYETIRVDPGKTYRLRVHNVGISTSLNF 244

Query: 246 RIQGHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASL 305
           RIQ HNMLL ETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVN SL
Sbjct: 245 RIQSHNMLLAETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNESL 304

Query: 306 WEKVTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSF 365
           W+KVTGVAIL YSNSKGPATGPLPDPPNDFYD+ERSMNQARSVRQNVSASGARPNPQGSF
Sbjct: 305 WQKVTGVAILQYSNSKGPATGPLPDPPNDFYDKERSMNQARSVRQNVSASGARPNPQGSF 364

Query: 366 HYGQINVTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPER 425
           HYGQINVTQTYLLK EPLVTIN+TARATFNGISF+PPKTPIRLADQHKVKGAYKLDFP+ 
Sbjct: 365 HYGQINVTQTYLLKSEPLVTINKTARATFNGISFIPPKTPIRLADQHKVKGAYKLDFPDM 424

Query: 426 PLNRTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSY 485
           PLNRTPRADISII+A YKGFIEVIFQNND+IIHS+H++GYSFFV GMGYGDWSEDKRGSY
Sbjct: 425 PLNRTPRADISIIDAAYKGFIEVIFQNNDSIIHSVHLDGYSFFVVGMGYGDWSEDKRGSY 484

Query: 486 NKWDAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKT 545
           NKWDAI R T+QVYPGAWTAVLISLDNVG WNLRAENLDRWYLGQETYLRIVNPEENGKT
Sbjct: 485 NKWDAITRSTSQVYPGAWTAVLISLDNVGVWNLRAENLDRWYLGQETYLRIVNPEENGKT 544

Query: 546 EMAPPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS 597
           EMA PSNVLYCGALQ+LQKEQ H NA+SIFKGHSKLFI+LLMA L++ F+ S
Sbjct: 545 EMAAPSNVLYCGALQSLQKEQHHGNANSIFKGHSKLFITLLMALLNVAFLLS 593

BLAST of MC10g0212 vs. ExPASy TrEMBL
Match: A0A6J1J899 (monocopper oxidase-like protein SKS1 OS=Cucurbita maxima OX=3661 GN=LOC111483473 PE=3 SV=1)

HSP 1 Score: 1093 bits (2828), Expect = 0.0
Identity = 522/592 (88.18%), Postives = 556/592 (93.92%), Query Frame = 0

Query: 6   RSSLSLFHLFPLLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPR 65
           R  LSLF+L P++ L   PS CSAADPY  YEFRVSYITASPLGVPQ+VIAVNG FPGP 
Sbjct: 5   RPVLSLFYLLPIIFL---PSFCSAADPYASYEFRVSYITASPLGVPQKVIAVNGNFPGPP 64

Query: 66  VNATTNYNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKD 125
           +N TTNYNVAVNVWNDLDENLL+TWSGIQMRRNSW DGVLGTNCPIPPKWNWTYQFQVKD
Sbjct: 65  INVTTNYNVAVNVWNDLDENLLMTWSGIQMRRNSWHDGVLGTNCPIPPKWNWTYQFQVKD 124

Query: 126 QIGSFYYFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRAD 185
           QIGSF+YFPS+NFQKA+GGFGPFVINNR+IIPIPFAQP+GDIFIMIGDW+ R+H ALRAD
Sbjct: 125 QIGSFFYFPSINFQKASGGFGPFVINNRDIIPIPFAQPEGDIFIMIGDWFTRDHAALRAD 184

Query: 186 LDAGKDLGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNF 245
           L AGK+LGIPDGVL+NGKGPYQYN TLVPAGIQYETI+VHPGKTYRLRVHNVGISTSLNF
Sbjct: 185 LSAGKELGIPDGVLINGKGPYQYNATLVPAGIQYETIRVHPGKTYRLRVHNVGISTSLNF 244

Query: 246 RIQGHNMLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASL 305
           RIQ HNMLL ETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYY+VASARFVN SL
Sbjct: 245 RIQSHNMLLAETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYVVASARFVNESL 304

Query: 306 WEKVTGVAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSF 365
           W+KVTGVAIL YSNSKGPATGPLPDPPNDFYD+ERSMNQARSVRQNVSASGARPNPQGSF
Sbjct: 305 WQKVTGVAILQYSNSKGPATGPLPDPPNDFYDKERSMNQARSVRQNVSASGARPNPQGSF 364

Query: 366 HYGQINVTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPER 425
           HYGQINVTQTYLLK EPLVTIN+T RATFNGISF+PPKTPIRLADQHKVKGAYKLDFP+R
Sbjct: 365 HYGQINVTQTYLLKSEPLVTINKTTRATFNGISFIPPKTPIRLADQHKVKGAYKLDFPDR 424

Query: 426 PLNRTPRADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSY 485
           PLNRTPRADISII+A YKGFIEVIFQNND+IIHS+H++GYSFFV GMGYGDWSED RGSY
Sbjct: 425 PLNRTPRADISIIDAAYKGFIEVIFQNNDSIIHSVHLDGYSFFVVGMGYGDWSEDLRGSY 484

Query: 486 NKWDAIARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKT 545
           NKWDAI R T+QVYPGAWTAVLISLDNVG WNLRAENLDRWYLGQETYLRIVNPEENGKT
Sbjct: 485 NKWDAITRSTSQVYPGAWTAVLISLDNVGVWNLRAENLDRWYLGQETYLRIVNPEENGKT 544

Query: 546 EMAPPSNVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFICS 597
           EMA PSNVLYCGALQ+LQKEQ H NA+SIFKGHSKLFI+LLMA L++ F+ S
Sbjct: 545 EMAAPSNVLYCGALQSLQKEQHHGNANSIFKGHSKLFITLLMALLNVAFLHS 593

BLAST of MC10g0212 vs. TAIR 10
Match: AT4G25240.1 (SKU5 similar 1 )

HSP 1 Score: 893.3 bits (2307), Expect = 1.1e-259
Identity = 421/578 (72.84%), Postives = 487/578 (84.26%), Query Frame = 0

Query: 17  LLLLLMLPSLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAV 76
           LL   +L ++  AADP++ Y+FRVSY+TASPLGVPQQVIAVNGQFPGP +NATTNYNV V
Sbjct: 12  LLCFALLSAVSFAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATTNYNVVV 71

Query: 77  NVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSL 136
           NV+N LDE LLLTW GIQMRRNSWQDGVLGTNCPIPP+WN+TYQFQVKDQIGSF+Y PSL
Sbjct: 72  NVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSFFYSPSL 131

Query: 137 NFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPD 196
           NFQ+A+GGFGP VINNR+IIPIPF QPDG++  +IGDWY ++H ALR  LD+GK+LG+PD
Sbjct: 132 NFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGKELGMPD 191

Query: 197 GVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVE 256
           GVL+NGKGPY+YN + VP GI Y T  V PGKTYR+RVHNVGISTSLNFRIQ H++LLVE
Sbjct: 192 GVLINGKGPYKYNSS-VPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSLLLVE 251

Query: 257 TEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILH 316
           TEGHYT   N+TDFD+HVGQSYSFLVTMDQ+A++DYYIVASARFVN ++W++VTGVAILH
Sbjct: 252 TEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTGVAILH 311

Query: 317 YSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTY 376
           YSNSKGP +GPLP P  D      +M+Q +++RQN SASGARPNPQGSFHYGQIN+T TY
Sbjct: 312 YSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQINITNTY 371

Query: 377 LLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNRTPRADIS 436
           +L+  P   IN   RAT NGISFV P TP+RLAD++KVKGAYKLDFP+RP NR  R D S
Sbjct: 372 ILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKGAYKLDFPDRPFNRPLRLDRS 431

Query: 437 IINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAIARCTT 496
           +INATYKGFI+V+FQNNDT I S H++GYSFFV GM +G WSEDK+GSYN WDAI+R T 
Sbjct: 432 MINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWDAISRSTI 491

Query: 497 QVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPSNVLYC 556
           +VYPG WTAVLISLDNVG WN+R ENLDRWYLG+ETY+RI NPEE+GKTEM PP NVLYC
Sbjct: 492 EVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDPPDNVLYC 551

Query: 557 GALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVF 595
           GAL+NLQKEQ H+ A SI  GH KL   LLM  L  VF
Sbjct: 552 GALKNLQKEQHHSAATSILNGHLKLM--LLMVLLASVF 586

BLAST of MC10g0212 vs. TAIR 10
Match: AT5G51480.1 (SKU5 similar 2 )

HSP 1 Score: 858.2 bits (2216), Expect = 3.9e-249
Identity = 397/564 (70.39%), Postives = 470/564 (83.33%), Query Frame = 0

Query: 29  AADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAVNVWNDLDENLLL 88
           A DPY+ Y+F +SYITASPLGVPQQVIAVNG+FPGP +NATTNYNV VNV N LDE LLL
Sbjct: 23  AGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLL 82

Query: 89  TWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNFQKAAGGFGPF 148
           TW G+QMRRNSWQDGVLGTNCPIPP WN+TY FQ+KDQIGS++Y PSLNFQ+A+GGFG  
Sbjct: 83  TWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGAL 142

Query: 149 VINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPDGVLMNGKGPYQY 208
           +INNR+++PIPF +PDG+I  +IGDWY +NHTALR  LD+GK+LG+PDGVL+NGKGP++Y
Sbjct: 143 IINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKY 202

Query: 209 NKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVETEGHYTVMQNYT 268
           N + VP GI++ET+ V PGKTYR+RVHNVGISTSLNFRIQ H +LL+ETEG YT   N+T
Sbjct: 203 NSS-VPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFT 262

Query: 269 DFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILHYSNSKGPATGPL 328
           DFD+HVGQSYSFLVTMDQNA++DYYIVASARFVN ++W++VTGV ILHYSNSKGPA+GPL
Sbjct: 263 DFDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPL 322

Query: 329 PDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTYLLKGEPLVTINR 388
           P    D      +MNQ R+++QN SASGARPNPQGSFHYGQIN+T+TY+L+  P   IN 
Sbjct: 323 PVSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPTKING 382

Query: 389 TARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLN-RTPRADISIINATYKGFIE 448
             RAT NGISFV P TP+RLAD HKVKG Y LDFP+RPL+ + PR   SIINATYKGFI+
Sbjct: 383 KLRATLNGISFVNPSTPMRLADDHKVKGDYMLDFPDRPLDEKLPRLSSSIINATYKGFIQ 442

Query: 449 VIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAIARCTTQVYPGAWTAVL 508
           VIFQNNDT I S H++GY+F+V  M +G WSED+  SYN WDA+AR T +VYPGAWTAVL
Sbjct: 443 VIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAWTAVL 502

Query: 509 ISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPSNVLYCGALQNLQKEQK 568
           ISLDNVG WN+R ENLDRWYLGQETY+RI+NPEENG TEM PP NV+YCGALQ +QKEQ 
Sbjct: 503 ISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCGALQAMQKEQH 562

Query: 569 HNNA-DSIFKGHSKLFISLLMAFL 591
           H++A  S+  G   L  S++M  L
Sbjct: 563 HSSATKSMTNGQLILIFSMMMVLL 585

BLAST of MC10g0212 vs. TAIR 10
Match: AT4G12420.1 (Cupredoxin superfamily protein )

HSP 1 Score: 842.0 bits (2174), Expect = 2.9e-244
Identity = 406/584 (69.52%), Postives = 465/584 (79.62%), Query Frame = 0

Query: 14  LFPLLLLLMLP--SLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTN 73
           LF +LLL+     S C AADPY FY F VSYITASPLGVPQQVIA+NG+FPGP +N TTN
Sbjct: 3   LFKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTN 62

Query: 74  YNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFY 133
            N+ VNV N LDE LLL W+GIQ RR SWQDGVLGTNCPIPPKWNWTY+FQVKDQIGSF+
Sbjct: 63  ENLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFF 122

Query: 134 YFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKD 193
           YFPSL+FQ+A+GGFG FV+N R IIP+PF+ PDGDI + IGDWY RNHTALR  LD GKD
Sbjct: 123 YFPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKD 182

Query: 194 LGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHN 253
           LG+PDGVL+NGKGPY+YN TLV  GI +ETI VHPGKTYRLRV NVGISTSLNFRIQGHN
Sbjct: 183 LGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHN 242

Query: 254 MLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTG 313
           ++L E+EG YTV QNYT  DIHVGQSYSFLVTMDQNAS+DYYIVASAR VN ++W +VTG
Sbjct: 243 LVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTG 302

Query: 314 VAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQIN 373
           V IL Y+NSKG A G LP  P D +D+  SMNQARS+R NVSASGARPNPQGSF YG IN
Sbjct: 303 VGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSIN 362

Query: 374 VTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNRTP 433
           VT  Y+L+  P VTI+   R T NGISF  P TPIRLAD+ KVK  YKLDFP+RPL    
Sbjct: 363 VTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKDVYKLDFPKRPLTGPA 422

Query: 434 RADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAI 493
           +   SIIN TY+GF+EV+ QNNDT + S HM+GY+FFV GM YG+W+E+ RG+YNKWD I
Sbjct: 423 KVATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGI 482

Query: 494 ARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPS 553
           AR T QVYPGAW+A+LISLDN G+WNLR ENLD WYLGQETY+R+VNP+EN KTE   P 
Sbjct: 483 ARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPD 542

Query: 554 NVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFI 596
           NVLYCGAL  LQK QK +++ S   G    F SL M  + +V +
Sbjct: 543 NVLYCGALSKLQKPQKVSSSASKSIG----FTSLSMVVMALVMM 582

BLAST of MC10g0212 vs. TAIR 10
Match: AT4G12420.2 (Cupredoxin superfamily protein )

HSP 1 Score: 842.0 bits (2174), Expect = 2.9e-244
Identity = 406/584 (69.52%), Postives = 465/584 (79.62%), Query Frame = 0

Query: 14  LFPLLLLLMLP--SLCSAADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTN 73
           LF +LLL+     S C AADPY FY F VSYITASPLGVPQQVIA+NG+FPGP +N TTN
Sbjct: 3   LFKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTN 62

Query: 74  YNVAVNVWNDLDENLLLTWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFY 133
            N+ VNV N LDE LLL W+GIQ RR SWQDGVLGTNCPIPPKWNWTY+FQVKDQIGSF+
Sbjct: 63  ENLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFF 122

Query: 134 YFPSLNFQKAAGGFGPFVINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKD 193
           YFPSL+FQ+A+GGFG FV+N R IIP+PF+ PDGDI + IGDWY RNHTALR  LD GKD
Sbjct: 123 YFPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKD 182

Query: 194 LGIPDGVLMNGKGPYQYNKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHN 253
           LG+PDGVL+NGKGPY+YN TLV  GI +ETI VHPGKTYRLRV NVGISTSLNFRIQGHN
Sbjct: 183 LGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHN 242

Query: 254 MLLVETEGHYTVMQNYTDFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTG 313
           ++L E+EG YTV QNYT  DIHVGQSYSFLVTMDQNAS+DYYIVASAR VN ++W +VTG
Sbjct: 243 LVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTG 302

Query: 314 VAILHYSNSKGPATGPLPDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQIN 373
           V IL Y+NSKG A G LP  P D +D+  SMNQARS+R NVSASGARPNPQGSF YG IN
Sbjct: 303 VGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSIN 362

Query: 374 VTQTYLLKGEPLVTINRTARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNRTP 433
           VT  Y+L+  P VTI+   R T NGISF  P TPIRLAD+ KVK  YKLDFP+RPL    
Sbjct: 363 VTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKDVYKLDFPKRPLTGPA 422

Query: 434 RADISIINATYKGFIEVIFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAI 493
           +   SIIN TY+GF+EV+ QNNDT + S HM+GY+FFV GM YG+W+E+ RG+YNKWD I
Sbjct: 423 KVATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGI 482

Query: 494 ARCTTQVYPGAWTAVLISLDNVGSWNLRAENLDRWYLGQETYLRIVNPEENGKTEMAPPS 553
           AR T QVYPGAW+A+LISLDN G+WNLR ENLD WYLGQETY+R+VNP+EN KTE   P 
Sbjct: 483 ARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPD 542

Query: 554 NVLYCGALQNLQKEQKHNNADSIFKGHSKLFISLLMAFLHIVFI 596
           NVLYCGAL  LQK QK +++ S   G    F SL M  + +V +
Sbjct: 543 NVLYCGALSKLQKPQKVSSSASKSIG----FTSLSMVVMALVMM 582

BLAST of MC10g0212 vs. TAIR 10
Match: AT5G48450.1 (SKU5 similar 3 )

HSP 1 Score: 726.1 bits (1873), Expect = 2.3e-209
Identity = 342/575 (59.48%), Postives = 432/575 (75.13%), Query Frame = 0

Query: 29  AADPYIFYEFRVSYITASPLGVPQQVIAVNGQFPGPRVNATTNYNVAVNVWNDLDENLLL 88
           AADPY+F+++ VSY++ASPLG  QQVI +NGQFPGP +N TTN+NV +NV N+LDE LLL
Sbjct: 24  AADPYVFFDWTVSYLSASPLGTRQQVIGINGQFPGPILNVTTNWNVVMNVKNNLDEPLLL 83

Query: 89  TWSGIQMRRNSWQDGVLGTNCPIPPKWNWTYQFQVKDQIGSFYYFPSLNFQKAAGGFGPF 148
           TW+GIQ R+NSWQDGVLGTNCPIP  WNWTY+FQVKDQIGSF+YFPS NFQ+A+GG+G  
Sbjct: 84  TWNGIQHRKNSWQDGVLGTNCPIPSGWNWTYEFQVKDQIGSFFYFPSTNFQRASGGYGGI 143

Query: 149 VINNREIIPIPFAQPDGDIFIMIGDWYARNHTALRADLDAGKDLGIPDGVLMNGKGPYQY 208
           ++NNR IIP+PFA PDGD+ + I DWY ++H  LR D+++   L  PDG+++NG GP+  
Sbjct: 144 IVNNRAIIPVPFALPDGDVTLFISDWYTKSHKKLRKDVESKNGLRPPDGIVINGFGPFAS 203

Query: 209 NKTLVPAGIQYETIQVHPGKTYRLRVHNVGISTSLNFRIQGHNMLLVETEGHYTVMQNYT 268
           N      G  + TI V PG+TYR RVHN GI+TSLNFRIQ HN+LLVETEG YT+ QNYT
Sbjct: 204 N------GSPFGTINVEPGRTYRFRVHNSGIATSLNFRIQNHNLLLVETEGSYTIQQNYT 263

Query: 269 DFDIHVGQSYSFLVTMDQNASTDYYIVASARFVNASLWEKVTGVAILHYSNSKGPATGPL 328
           + DIHVGQS+SFLVTMDQ+ S DYYIVAS RF  +    K +GVA+L YSNS+GPA+GPL
Sbjct: 264 NMDIHVGQSFSFLVTMDQSGSNDYYIVASPRFATSI---KASGVAVLRYSNSQGPASGPL 323

Query: 329 PDPPNDFYDRERSMNQARSVRQNVSASGARPNPQGSFHYGQINVTQTYLLKGEPLVTINR 388
           PDPP +  D   SMNQARS+R N+S+  ARPNPQGSF YGQI VT  Y++   P   I  
Sbjct: 324 PDPPIEL-DTFFSMNQARSLRLNLSSGAARPNPQGSFKYGQITVTDVYVIVNRPPEMIEG 383

Query: 389 TARATFNGISFVPPKTPIRLADQHKVKGAYKLDFPERPLNRTPRADISIINATYKGFIEV 448
             RAT NGIS++PP TP++LA Q+ + G YKLDFP+RP+NR PR D S+IN T+KGF+E+
Sbjct: 384 RLRATLNGISYLPPATPLKLAQQYNISGVYKLDFPKRPMNRHPRVDTSVINGTFKGFVEI 443

Query: 449 IFQNNDTIIHSIHMNGYSFFVAGMGYGDWSEDKRGSYNKWDAIARCTTQVYPGAWTAVLI 508
           IFQN+DT + S H++GY+FFV GM +G W+E+ R +YNK DA+AR TTQV+PGAWTAVL+
Sbjct: 444 IFQNSDTTVKSYHLDGYAFFVVGMDFGLWTENSRSTYNKGDAVARSTTQVFPGAWTAVLV 503

Query: 509 SLDNVGSWNLRAENLDRWYLGQETYLRIVNPE-ENGKTEMAPPSNVLYCGALQNLQKEQK 568
           SLDN G WNLR +NL  WYLGQE YL +VNPE +   +E + P N +YCG L  LQK+Q 
Sbjct: 504 SLDNAGMWNLRIDNLASWYLGQELYLSVVNPEIDIDSSENSVPKNSIYCGRLSPLQKDQA 563

Query: 569 H-----NNADSIFKGHSKLFISLLMAFLHIVFICS 598
                  +  SIF     + ++L    ++I  +C+
Sbjct: 564 QRVNFSGSQRSIFVTSRGILLALFAILVNINRLCN 588

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VXX51.5e-25872.84Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Q9FHN65.5e-24870.39Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Q9SU404.1e-24369.52Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q006241.3e-14044.73L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291624.8e-13544.38L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022140913.10.0100.00monocopper oxidase-like protein SKS1 [Momordica charantia][more]
XP_038880777.10.091.34monocopper oxidase-like protein SKS1 [Benincasa hispida][more]
XP_008439091.10.090.52PREDICTED: monocopper oxidase-like protein SKS1 [Cucumis melo][more]
KAG6576799.10.088.85Monocopper oxidase-like protein SKS1, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_011651117.10.090.85monocopper oxidase-like protein SKS1 [Cucumis sativus] >KGN57269.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
A0A6J1CJ410.0100.00monocopper oxidase-like protein SKS1 OS=Momordica charantia OX=3673 GN=LOC111011... [more]
A0A1S3AXI50.090.52monocopper oxidase-like protein SKS1 OS=Cucumis melo OX=3656 GN=LOC103483983 PE=... [more]
A0A0A0L8C40.090.85Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G175700 PE=3 SV=1[more]
A0A6J1E5V70.088.51monocopper oxidase-like protein SKS1 OS=Cucurbita moschata OX=3662 GN=LOC1114308... [more]
A0A6J1J8990.088.18monocopper oxidase-like protein SKS1 OS=Cucurbita maxima OX=3661 GN=LOC111483473... [more]
Match NameE-valueIdentityDescription
AT4G25240.11.1e-25972.84SKU5 similar 1 [more]
AT5G51480.13.9e-24970.39SKU5 similar 2 [more]
AT4G12420.12.9e-24469.52Cupredoxin superfamily protein [more]
AT4G12420.22.9e-24469.52Cupredoxin superfamily protein [more]
AT5G48450.12.3e-20959.48SKU5 similar 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 167..319
e-value: 5.8E-44
score: 150.0
IPR008972CupredoxinGENE3D2.60.40.420coord: 163..356
e-value: 5.2E-42
score: 146.2
IPR008972CupredoxinGENE3D2.60.40.420coord: 369..570
e-value: 5.1E-33
score: 116.5
IPR008972CupredoxinGENE3D2.60.40.420coord: 28..152
e-value: 3.4E-30
score: 106.9
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 160..359
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 386..563
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 29..180
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 40..154
e-value: 4.8E-40
score: 136.3
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 413..540
e-value: 1.5E-25
score: 89.6
NoneNo IPR availablePANTHERPTHR11709:SF270MONOCOPPER OXIDASE-LIKE PROTEIN SKS1coord: 18..568
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 18..568
IPR034273Ascorbate oxidase homologue, first cupredoxin domainCDDcd13846CuRO_1_AAO_like_1coord: 34..151
e-value: 9.45601E-78
score: 240.002

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0212.1MC10g0212.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity