MC10g0202 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0202
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionMitochondrial intermediate peptidase, mitochondrial
LocationMC10: 1467920 .. 1476364 (-)
RNA-Seq ExpressionMC10g0202
SyntenyMC10g0202
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CGGCAAACGCACACACACTCGGACCTCGAAGCCGCCGCCTCCGCCGCCGCATGTCGAGCCTGATCCGCAGAACCACCCCGAAGCTCAGTTCGGGAGCAGCTCTAAAATCATGCTTCTCGAATCGCCATAGGTCCCGCCGTTCGATCCACGCCCCAGCCTCAGCTCCGCAGAAAGGTGCTGCAACCGGTCTTTATGGCTTTGAGCACCTCAAGTCGCCTAAAGGTTTCCGACGTTTCGTCGATGAAGCCATTGAGAGGTAACAACATTAACGCTAGAAGTTGAAAGCCTTTAATGCTTGAGAGATGAGACTCAATTTGGAAGCCAAAGTGAAGAAACTGGAAGTATTAACTTCTGTATTGTGAAGCATAGAGATTACTTAACATCTTTGTAGCTAATTTGGTTCCTTGTTCTGTCAGAGTTGCTTGATCAACAAGATAATGCCCGGAAAATGTAACAACGATTTTTTCTGACAATTTTTATTGTTCTGGTCTTCTGTACCATCGATTAGCTTAACACTAAAACAACTGAGCTTAGTTATGTATATGACAGAGTGGAGGAATTCTCATTCTATGTAAATTTTAAGGAAGTTCAAGGGGGCTTTTATCTACGGGGTTTTTTTTTCTTTGAATTGGGGGAGTAAAGAAAAGTGCAAGATACAAAGGATTTCGAAACAAAAATCATCACGTCTTATATTGTATCATATGGGAAGTCATGTGTTCTGGGTTTGGGAGCCACTTTCTTGAGGTATTAATATAATAATCCATGCGTTTTCCAACACGTCAGCTGACTTGAACACATGGATACTCAAAAAATAACTCATCACCATGTTACAACAAATATCTAAATTCATTGATAGCGAGAGGATGCTTGGAACCTTTATCTAATTGAAACAGCAAGGAAAAAATTACTTTCTTCCCTTTGAAAAATAATTTATCCGTTTTTTAGGTCTGGCGAGCTTGTTACTTTCATTTCTAGCAAGCCTTCTTCAGCAGAAGTTATCCGAGCAATGGATGAAATTTCCAACAGAGTAGGTTTAGAATTTTGGATTTACTTTGAGTTGTAATTTTGAACCATCTGTCGGATATATTTCAGCTTCCATGTAAGAATTCTTGGTTTAATTGTGGTATTTATTGATTAGGTGTGCTCTGTTTTTGATTCTGCAGAACTTTGTAGGCAAACTCATCCAGACAGGTTTGTTACTTTTCAGATAATTTTTGCACCTTGGTTATCGCTATTGTTATTCATATTGCTTTGCCAATCAGTCTAGCAGTTGTTCCTGTTAAATGACTGTTTGCTACAATTTTTTCACGTAATAGGGAATTTGTGGAGGAGGCAAACAAGGCAGCTATGAGATTGAACGAATATTTTCATGTGAGGAGCCCAGCAAACCATATCTTGAATGTGACTTTTTTTATTTTCTTTGTCAGTAGTCAGTATTACTGGTTTGCCAACTAATTCATTATCACTGTCAAGTCTGAGCTTTATCATCAGTGAAAACCAAACATGTGTTCTGGAATAGTGTATTCTTCTCCACTTCAACTCTAGTGCCAAAATATTATTTAAAATTTTAAGAATAACTAATTTGGTGAGCTGTAGCGGTAAAATGTGATATACTATCAGGACTGAATTGCCTCTTTTATTATATAAACCTTCTTTGAATAGTAATTCGGACAGTGGACTTGCCAGTCTCGGAAAACAGCCTAGTTTACAGGCTTCATCCTAAATGTTATCTCTCATGATCACCATTATAAGATGACAACTTTTAAAATTTTATACTTGATTAGTTTTATTAAACTGTTTCTTTCTCTCTCCAACATCCCTTTGAACCTGCGACATGTGGGTTAAGTAACCATGAAGCCTACGAGCTACAGAGAGAATCAATATTACTTGACTAATTATGAGATAGCAAGTTTTAACTATTAAACTTTTTTACTTGATTTACATCTGTTATTTTAGTAACCTGAAGCTACTGAAACTGAAAAGTACTTAACGTAAGTAACTCGAACCTACTGATTGAAAAATACTTTAACATTTGAATTTGAACAAAATGGATAAAGACTTTTGCTGTAGTTAAATTGAAAACAAAATCTGGAAAAAACTTTGTATTTGGATCTGAAAAGAAAATATTGAGATGAGTTACATATGCTTATATATCAGTTTTATCCGTGCCTTCCTTAGCCTTACATAATATAGAGTAGGAAAACTAAAATGATCACCAAGCTTCCTTTGTACTTTTCTATTTTGCATGAGAATTGTTCTTGGCTGTTTGAAAACTTAGTACCCAAACGTTAATTCATGTTTTCTTTATGTTTTATAGTATCTCAATACAAATCATACTTTGTATAATGCTGTAAAAAAAGCTGAGCATGAGGCTCATCTACTTACACGTGAAGCTCATAAGGCTGCCCATTACCTCCGTGTTGACTTTGAAAGGGCTGGAATTCATCTTTCTGCTGGTACTTTCTGTTTGTAGTTCACTCCGTGCTTCTTACTCATGCTTCTGCATTTAAAATTTAACACCTTAATTATGCTTATGCAGAGAGATTAGATCGAGTAAATCAGCTAAGTATAGAAATTTCTCAGCTATGTCAGGAGTAAGTTGCATGTAATAGTCGCTAAATTATATTTGCTTTGCTCTTCATATCATTTTCATGCTTCTTTCAATGATATTATCTGCACATCAATTAAAAAAAGAAAAAGAGAAGAAAAAAGAAAAAAAAAAGAGAAATAAAAGGAGTCACTAGCTGTCACTATAAACTGTACATAATACTACGGTCACAAAATTCAAGTGGTCTTCAATAAATTGATTGGATCATACTTTAACTTCAGAGCTGTTGTAAAGTTGTAATATTTGGTGAATGCTCTTCTTTATCAGTTATCGAGAAACCTCATTATATTTATACTGTTATATGCAAGTATTTCTCAACGACCTAGGCCATTTCTTACCTATTGGAGGGGAAGAAGAGAAAAAGAAAGATGTTCCATGCATCTTTGTTAATTTTGTTGAATATGTTTCATTTGGTCAGGTTCAAAGAAAATATCGTCATTGACCCAGGCTCTGTAGATATATTTCCACCATTGTGTATGCCAAACAAGTTGCACCATCTTGTTAAGCCCATCTATCGTTCTAGTGATTCATTTGGGTCAAGAAAAGGCATGAAAGAAAATGGATTTCGATTAGTGACTGATGCAGATACTCTATATTCTGTTCTACAATACGCATCAGATGATGAGGTGGGTAGTCCTGTTAAGTGTTAGTTGTATAGTAATGCACGATGTGTTTTCAGTGGCTTGGGGTACTTTGAAGGATGTCGAAGTTTCCATAAATTCTTGTAGGTTGAAGCCCTATGATGCATTTTTTAAGTCAAACAGATTTAGATATCTGAGGAAGGAGATCTTATAAAACATATGATGAGATGTTTCAGAAATAATGTTTTGCTAAGGCATCAATTTTTTTCATGATTAGGCTTGATCCTTTTTCTGATTAACAATTTTTTTTTTTTTTTTTTGCACTTAAAACACTTTTTCAGGTAAGGAAAATGGCATATATCAAGGGAAATTCAAGCCCTCGTGCTAACCTTGATGTTCTTGATAAGCTTATTGCCACTCGTCACGAGCTAGCTCAGGTTATTCATTTTCATTTTCCACTCTCTGTAATTTGTAAAGAACAATGTAATATGTAACATTTCTTCTCTTCCCTGTCTTCCATTATTCAATATTGAATTCTTAAAGTTATTCTTTCCTTGGAACGAGTCATTCAAATATTGTGGTGCAAAACTTTTCATCATTCTTTTATGAGGACAATAGCCATGTGAACTTGTTTCCTGCAGAGGATTCGTATTCAATCATTTATGGCAACAATTTGCTTCTTGCTTGGGTTTGGTTCCCTAGCCCATCCCTATAGCTCTTTTTCTCACTTGTAGATTAATTCCAAAAAAAGGAAGATCACCTCTCAACCTTTTTCAATCATATGTGAAGTGAACACGACAGGCAGCTTGCCATTTCTGTCATCTCAATCACAGTGACACTTTGAGAAATTCTGGAGAAATATTCGATTAATAGAACAGAATTGTGTAATAAATTTATCAAATAACTTGAGATTATCGAGAAATTTCTGTTTGTTCCCATTTGTGAAAAAGGTTAAAGAAAAAATTTCTTATATATGGAAAAAGGATCATGTTACAGTGCATTACCCCCGATATCTTAGCCTTTTGCTTCATGTTGATTATGCAGATAGTAGGGTATAGATCTTATGCAGAATTTGCAGTAACGCCTAACTTGGCTTCATCTCCAGCTGTGGTAATGTCCTTTTTGCAAGAGTTGAGCAAAGTAGTCCGTCCCTGTGCCGATGAGGTACTTCAATTTTTTTTTTTTGAGACTTTGCAATTTGCAAGGCATGGAACCGTTGTTTCTCTGTCATCATAACTTGTTGAACTTCTGCTTTCATATTTTAACAAGAAGTTGTAACCGCCTTCAGGAGTTCAATCAAATCAGGGAATTTAAGTTAAAGAAGTGCGTTAATAAGTCTGAAGATTTAGAACCATGGGATGAGGCTTACTATACATCAATGATGAAATCTACTGCATATAACCTGGATTCATCGGCAAGTTACATCTTCTATTTCTTTCTTTCTTTCTTTCTCTCTCTTTTTTCTTTAAAAAAAAATGTGCAACGTTGTTCCTTGTTCACATTTGTAAAGCAGGGTTCTTTGACGCTTTTTTTTTTGTCCACTGAGATTCTCATAGTATCCTTTTCATGCTCCTAACTGCTAGTATTATTATGGCACTCTCTTCTTTTTCTAGGTTGTAGCATCATACTTTCCTTTATCACAGTGTATTGAGGGTTTGAAAATTCTCGTGGAGTCATTGTTTGGCGCATCCTTTTATAGTATTCCCCTAGCACCAGGTGAATCATGGCATCCTGACGTGCTTAAATTGTCTCTTCATCATCCTGAAGAGGTATATACTACATAATATTAACTGTAGATTTCTTCTTCGTTGGTTATAAATTGTAACGGAATTAGACTGTTGTTAACTGAAACTTCATCCATTAAGTTTTCTGATTTCCGTTTTGTACAAAGCCCCAATACTTAATATGGTTACTAGGAATTATTACAATTAGTAATGTTTATTTAATGTGAGCATTTTCTTGTGTGAGTAAAAAGGTGCATGGTACTTTTTTTTTAAAAGAAAGGTAATCACGATGATCTTTGTAGTTATAATTGTAGGAAGCAGTTATGATTTCTGGATGGAGGAGTATCATTAAATCTGTTTCTATCTTTTCTGGAACAACACCAGTTCCCCTGAAACAAAGTGGAGACTGTGGTGATAATCGTTTGTGATTCATATATTCAAAGATAAATATTTTTAATCAATATTAGAGATTCCATCACATCTATAGTCCTGAAGTGAGAAACTACCAGATCAAATCTTGAAAGGATGTACATGACCGTCTCTTGAGATCAAATCCTCGGGGCTCAGTTTATGAGTTATGGTGCGGACAGTTTTTGCAACTTAGTTTTCACTTAAAAGTTTTCATTGATGCACATACTTAAACTTTCCCCCCTTTCTGCAAACATCAAAATTACTTCCAAACTCATAGTGCCACCATATTTCTGTTTCAGGGTGACTTGGGATATTTATACCTGGATTTGTACTCAAGAAAAGGAAAATATCCAGGTTGTGCTCATTTTGCAATTAGGGGAGGCCGCAAGGTTTCTGAAATGGAATACCAACTTCCTGTATGTATTAGAATTGAACTTCATACGTGATAGCAACTTTTGATTTCTGTAATACGTCGTCCTACAGCTGTATGCCTGTATCTGGTTAAACATTTTTGGGATGCTTCAATTATTGTTTCTGGACAATTGCAAAGACACGGAATCCTCTTATAGCTATTATTAATCATGCTGTAATTGAGAATAATGTGAAAATCTACTTATGCTTCTAATGTGAGATAGAAAGTTCATTGTACGGTGAAGGGGATTGACATTGCTTATTATTATGATGGTGTATGCCCTGATGGGAACGTGCTTATGGTTTTTGTTTGCTAATGTTAGACTAATGTCTGGCTAATTTAATGGTCTTGCGTGTTCTCTTTCAACTTGTAATCAAAACCAAAAGGTTTATAATTTAATGCTAAAAATATATGCATTTAAGAAGGCTCATGAGGAAAAAGTTTTTAGAAATGTAAAGCAGTAGACTGATTTAGTGACCTTCCAAATCAAAAGTCTGTACTGGTTATGTGCAGGTTGTAGCTCTTGTTTGCAATTTTTCCAGTTCCCATGGTCGATCAAATGTGAGGCTTAATCATTCGGAAGTAGAAACTCTTTTCCACGAATTTGGACATGCTCTTCATTCACTGCTTTCAAGAACGGTTTGTGCTGATTCTTTACTCTTGAACAGACATGTCTAACACCTCCTCTGGGTTTCTTTGTTTCTTGTTATACAACTCATCAGTTCTCAACTACTGCAGGAATATCAACATTTTTCAGGTACAAGAGTGGTTCTTGATCTGGCAGAGACACCTTCAAACCTATTTGAGTGAGTAGAAATGTCCCGCTTATTTCTTGCCTTTTGGTTTATGAAGATCGTATAAAGGTTTTGAATCACCTCTATGCTTAATTATTCTTATACTTAAGCTATAATGTGTTTACTATGGCTTTTCTAAGAAAGATGCCCATTTATCTTTATCGTATATGAACGTCAAATTACGCAATAAAAAATGTCCAGAATGTCCTAGTCTAAATTCTTAGTAAGAGATAATAAATCAACAAATTTATATAATTACTCTCTTGTAAATATGATGATAAAGGTAATGAGCCAACAGCATGGATAAAATCACCCTGCTGTCTAGAATAAGTTGTTCAAGTGTGTAATCTTGTACATATAACTTGACGAACTGTTTCTCTTATAAGTCATTTTTTTATATGAGCGGTGCTCTGTGCTTCTTGTTGCTTCATAAGAGCTATCACCGATATTTGATGACTAGGTACTATGCATCAGATTATCGTGTTTTGAGAACATTTGCAAAGCACTATTCAACTGGTGATATAATTCCTGAAAAGCTTGTAAAGTCAATGCAGGGTGCCAAAAAGATGTTTGAAGCCACTGAACTGCAGCGTCAGGTAGCATGTTGCTGGTATTGTTAACTTTGAGCATAGCTTATATTTCAATCTGAACTAACCAATTTTTACCTGTCTCTTCGGTTACCAGATTCTTTATGCTTTAATTGATCAAACACTGTTTGGAGAAAGGTTAACTTCAGAGAGAGATACAAGTTCTGTTGTTGCAGAGCTAAAAAGACAATATACTAATTGGAAGCATGTGGATGACACTCATTGGCAGACCCGATTTTGTCACCTCCTGACTTATGGTGCAGGTAGCAAAAGTTTATCTATTTTTCAGTTTGAAATGTGAAATATTTTCGCTCCTCATTTTGATATGAGATCTCTAATCTCGAGTGACCGATTTGATCTCTTTGCTACCAGTTGGATCGAGGAACAAAGAGTATCGATTCTCTTGGCATCATTCTAACTAATAAGTGCTAATTTCTTAAGCCATTCGAGCATGCTTGCATAATAGTAATTGTAGATTGGAGTTCATAATAACCAAAATACCCACCTAAACTATTTCCTGGACCAACTTGAATTGCTTTTTTTTTTCTTTACTAAGATTTTACAAAAACTTAAACAGCATGCAATTGAGTTATCCAAATAATTTCCTTATCTCTCTTTTTTTCCATAAACAATTCATTCCAAGAAATGGAAAATCCAAATTCTATGCAACTTCAACTTTAGTCATATCATACCCGAATTCTGGATTCATGTTGTGAAATCAACTATCGGCTGAACCAGTACCCTGAGTAGCAGTTTATATGAATCAATCATGGAATGTTCATTTCATCTCTGTGGTGTGAGATATTTACAGGTCTGTTCTTACAGGTTACTACAGCTACCTTTATGCGAAATGTTTTTCTGCAACCATATGGGAGAAGCTTTGTCAGGAGGATCCTCTTTCAAGAAACACAGGAACTGCCTTGAGAACAAAATTTTTACAACATGGCGGCTCCAAAGAAGCAGTTGATCTATTAACTGATCTCGTGGGAGATGGGATCGTAAGGTATTGTGATGGAGGAGTAATTCCTGACCTAACGAGTCTTTGCAAGGAAATGGATCTTAGTGGAAGCTTGTAGCTAAAGGCCTTTCGCCATTGTCTTTTCCTTTCTAGAAATTATTGTTCTTCCATTTTTGGTCCTCGATGTATCATTTGGAAGCTCATTCATTGTTAAGTTTTAACTTGATGATTCTCATCACCTTCTCTGTTCTGTTCTGTTGTTGAAGTTCATAAGCTTCAATTTGTAAGCATTCTTTGTAATGGCTGTTCTATTTTATTTATCTTAATAAAAGTTCTGTTTTACACTAAAAAAAAAAAAAAAAATTTTGCA

mRNA sequence

CGGCAAACGCACACACACTCGGACCTCGAAGCCGCCGCCTCCGCCGCCGCATGTCGAGCCTGATCCGCAGAACCACCCCGAAGCTCAGTTCGGGAGCAGCTCTAAAATCATGCTTCTCGAATCGCCATAGGTCCCGCCGTTCGATCCACGCCCCAGCCTCAGCTCCGCAGAAAGGTGCTGCAACCGGTCTTTATGGCTTTGAGCACCTCAAGTCGCCTAAAGGTTTCCGACGTTTCGTCGATGAAGCCATTGAGAGGTCTGGCGAGCTTGTTACTTTCATTTCTAGCAAGCCTTCTTCAGCAGAAGTTATCCGAGCAATGGATGAAATTTCCAACAGAGTGTGCTCTGTTTTTGATTCTGCAGAACTTTGTAGGCAAACTCATCCAGACAGGGAATTTGTGGAGGAGGCAAACAAGGCAGCTATGAGATTGAACGAATATTTTCATTATCTCAATACAAATCATACTTTGTATAATGCTGTAAAAAAAGCTGAGCATGAGGCTCATCTACTTACACGTGAAGCTCATAAGGCTGCCCATTACCTCCGTGTTGACTTTGAAAGGGCTGGAATTCATCTTTCTGCTGAGAGATTAGATCGAGTAAATCAGCTAAGTATAGAAATTTCTCAGCTATGTCAGGAGTTCAAAGAAAATATCGTCATTGACCCAGGCTCTGTAGATATATTTCCACCATTGTGTATGCCAAACAAGTTGCACCATCTTGTTAAGCCCATCTATCGTTCTAGTGATTCATTTGGGTCAAGAAAAGGCATGAAAGAAAATGGATTTCGATTAGTGACTGATGCAGATACTCTATATTCTGTTCTACAATACGCATCAGATGATGAGGTAAGGAAAATGGCATATATCAAGGGAAATTCAAGCCCTCGTGCTAACCTTGATGTTCTTGATAAGCTTATTGCCACTCGTCACGAGCTAGCTCAGATAGTAGGGTATAGATCTTATGCAGAATTTGCAGTAACGCCTAACTTGGCTTCATCTCCAGCTGTGGTAATGTCCTTTTTGCAAGAGTTGAGCAAAGTAGTCCGTCCCTGTGCCGATGAGGAGTTCAATCAAATCAGGGAATTTAAGTTAAAGAAGTGCGTTAATAAGTCTGAAGATTTAGAACCATGGGATGAGGCTTACTATACATCAATGATGAAATCTACTGCATATAACCTGGATTCATCGGCAACATCATACTTTCCTTTATCACAGTGTATTGAGGGTTTGAAAATTCTCGTGGAGTCATTGTTTGGCGCATCCTTTTATAGTATTCCCCTAGCACCAGGTGAATCATGGCATCCTGACGTGCTTAAATTGTCTCTTCATCATCCTGAAGAGGGTGACTTGGGATATTTATACCTGGATTTGTACTCAAGAAAAGGAAAATATCCAGGTTGTGCTCATTTTGCAATTAGGGGAGGCCGCAAGGTTTCTGAAATGGAATACCAACTTCCTGTTGTAGCTCTTGTTTGCAATTTTTCCAGTTCCCATGGTCGATCAAATGTGAGGCTTAATCATTCGGAAGTAGAAACTCTTTTCCACGAATTTGGACATGCTCTTCATTCACTGCTTTCAAGAACGGAATATCAACATTTTTCAGGTACAAGAGTGGTTCTTGATCTGGCAGAGACACCTTCAAACCTATTTGAGTACTATGCATCAGATTATCGTGTTTTGAGAACATTTGCAAAGCACTATTCAACTGGTGATATAATTCCTGAAAAGCTTGTAAAGTCAATGCAGGGTGCCAAAAAGATGTTTGAAGCCACTGAACTGCAGCGTCAGATTCTTTATGCTTTAATTGATCAAACACTGTTTGGAGAAAGGTTAACTTCAGAGAGAGATACAAGTTCTGTTGTTGCAGAGCTAAAAAGACAATATACTAATTGGAAGCATGTGGATGACACTCATTGGCAGACCCGATTTTGTCACCTCCTGACTTATGGTGCAGGTTACTACAGCTACCTTTATGCGAAATGTTTTTCTGCAACCATATGGGAGAAGCTTTGTCAGGAGGATCCTCTTTCAAGAAACACAGGAACTGCCTTGAGAACAAAATTTTTACAACATGGCGGCTCCAAAGAAGCAGTTGATCTATTAACTGATCTCGTGGGAGATGGGATCGTAAGGTATTGTGATGGAGGAGTAATTCCTGACCTAACGAGTCTTTGCAAGGAAATGGATCTTAGTGGAAGCTTGTAGCTAAAGGCCTTTCGCCATTGTCTTTTCCTTTCTAGAAATTATTGTTCTTCCATTTTTGGTCCTCGATGTATCATTTGGAAGCTCATTCATTGTTAAGTTTTAACTTGATGATTCTCATCACCTTCTCTGTTCTGTTCTGTTGTTGAAGTTCATAAGCTTCAATTTGTAAGCATTCTTTGTAATGGCTGTTCTATTTTATTTATCTTAATAAAAGTTCTGTTTTACACTAAAAAAAAAAAAAAAAATTTTGCA

Coding sequence (CDS)

ATGTCGAGCCTGATCCGCAGAACCACCCCGAAGCTCAGTTCGGGAGCAGCTCTAAAATCATGCTTCTCGAATCGCCATAGGTCCCGCCGTTCGATCCACGCCCCAGCCTCAGCTCCGCAGAAAGGTGCTGCAACCGGTCTTTATGGCTTTGAGCACCTCAAGTCGCCTAAAGGTTTCCGACGTTTCGTCGATGAAGCCATTGAGAGGTCTGGCGAGCTTGTTACTTTCATTTCTAGCAAGCCTTCTTCAGCAGAAGTTATCCGAGCAATGGATGAAATTTCCAACAGAGTGTGCTCTGTTTTTGATTCTGCAGAACTTTGTAGGCAAACTCATCCAGACAGGGAATTTGTGGAGGAGGCAAACAAGGCAGCTATGAGATTGAACGAATATTTTCATTATCTCAATACAAATCATACTTTGTATAATGCTGTAAAAAAAGCTGAGCATGAGGCTCATCTACTTACACGTGAAGCTCATAAGGCTGCCCATTACCTCCGTGTTGACTTTGAAAGGGCTGGAATTCATCTTTCTGCTGAGAGATTAGATCGAGTAAATCAGCTAAGTATAGAAATTTCTCAGCTATGTCAGGAGTTCAAAGAAAATATCGTCATTGACCCAGGCTCTGTAGATATATTTCCACCATTGTGTATGCCAAACAAGTTGCACCATCTTGTTAAGCCCATCTATCGTTCTAGTGATTCATTTGGGTCAAGAAAAGGCATGAAAGAAAATGGATTTCGATTAGTGACTGATGCAGATACTCTATATTCTGTTCTACAATACGCATCAGATGATGAGGTAAGGAAAATGGCATATATCAAGGGAAATTCAAGCCCTCGTGCTAACCTTGATGTTCTTGATAAGCTTATTGCCACTCGTCACGAGCTAGCTCAGATAGTAGGGTATAGATCTTATGCAGAATTTGCAGTAACGCCTAACTTGGCTTCATCTCCAGCTGTGGTAATGTCCTTTTTGCAAGAGTTGAGCAAAGTAGTCCGTCCCTGTGCCGATGAGGAGTTCAATCAAATCAGGGAATTTAAGTTAAAGAAGTGCGTTAATAAGTCTGAAGATTTAGAACCATGGGATGAGGCTTACTATACATCAATGATGAAATCTACTGCATATAACCTGGATTCATCGGCAACATCATACTTTCCTTTATCACAGTGTATTGAGGGTTTGAAAATTCTCGTGGAGTCATTGTTTGGCGCATCCTTTTATAGTATTCCCCTAGCACCAGGTGAATCATGGCATCCTGACGTGCTTAAATTGTCTCTTCATCATCCTGAAGAGGGTGACTTGGGATATTTATACCTGGATTTGTACTCAAGAAAAGGAAAATATCCAGGTTGTGCTCATTTTGCAATTAGGGGAGGCCGCAAGGTTTCTGAAATGGAATACCAACTTCCTGTTGTAGCTCTTGTTTGCAATTTTTCCAGTTCCCATGGTCGATCAAATGTGAGGCTTAATCATTCGGAAGTAGAAACTCTTTTCCACGAATTTGGACATGCTCTTCATTCACTGCTTTCAAGAACGGAATATCAACATTTTTCAGGTACAAGAGTGGTTCTTGATCTGGCAGAGACACCTTCAAACCTATTTGAGTACTATGCATCAGATTATCGTGTTTTGAGAACATTTGCAAAGCACTATTCAACTGGTGATATAATTCCTGAAAAGCTTGTAAAGTCAATGCAGGGTGCCAAAAAGATGTTTGAAGCCACTGAACTGCAGCGTCAGATTCTTTATGCTTTAATTGATCAAACACTGTTTGGAGAAAGGTTAACTTCAGAGAGAGATACAAGTTCTGTTGTTGCAGAGCTAAAAAGACAATATACTAATTGGAAGCATGTGGATGACACTCATTGGCAGACCCGATTTTGTCACCTCCTGACTTATGGTGCAGGTTACTACAGCTACCTTTATGCGAAATGTTTTTCTGCAACCATATGGGAGAAGCTTTGTCAGGAGGATCCTCTTTCAAGAAACACAGGAACTGCCTTGAGAACAAAATTTTTACAACATGGCGGCTCCAAAGAAGCAGTTGATCTATTAACTGATCTCGTGGGAGATGGGATCGTAAGGTATTGTGATGGAGGAGTAATTCCTGACCTAACGAGTCTTTGCAAGGAAATGGATCTTAGTGGAAGCTTGTAG

Protein sequence

MSSLIRRTTPKLSSGAALKSCFSNRHRSRRSIHAPASAPQKGAATGLYGFEHLKSPKGFRRFVDEAIERSGELVTFISSKPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEANKAAMRLNEYFHYLNTNHTLYNAVKKAEHEAHLLTREAHKAAHYLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEFKENIVIDPGSVDIFPPLCMPNKLHHLVKPIYRSSDSFGSRKGMKENGFRLVTDADTLYSVLQYASDDEVRKMAYIKGNSSPRANLDVLDKLIATRHELAQIVGYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCADEEFNQIREFKLKKCVNKSEDLEPWDEAYYTSMMKSTAYNLDSSATSYFPLSQCIEGLKILVESLFGASFYSIPLAPGESWHPDVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIRGGRKVSEMEYQLPVVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEATELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQYTNWKHVDDTHWQTRFCHLLTYGAGYYSYLYAKCFSATIWEKLCQEDPLSRNTGTALRTKFLQHGGSKEAVDLLTDLVGDGIVRYCDGGVIPDLTSLCKEMDLSGSL
Homology
BLAST of MC10g0202 vs. ExPASy Swiss-Prot
Match: F4KDA5 (Mitochondrial intermediate peptidase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OCT1 PE=3 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 1.8e-274
Identity = 463/679 (68.19%), Postives = 551/679 (81.15%), Query Frame = 0

Query: 42  GAATGLYGFEHLKSPKGFRRFVDEAIERSGELVTFISSKPSSAEVIRAMDEISNRVCSVF 101
           G ATGLYGF+HLK+ KGF+RFV +AIERSGELV++IS  PSS E+I+AMDEIS+ VC V 
Sbjct: 28  GDATGLYGFDHLKTAKGFQRFVADAIERSGELVSYISGMPSSPEIIKAMDEISDTVCCVV 87

Query: 102 DSAELCRQTHPDREFVEEANKAAMRLNEYFHYLNTNHTLYNAVKKAEHEAHLLTREAHKA 161
           DSAELCRQTHPDREFVEEANKAA+ +N+Y H+LNTNHTLY AVKKAE +++LLT+EA + 
Sbjct: 88  DSAELCRQTHPDREFVEEANKAAIEMNDYLHHLNTNHTLYAAVKKAEQDSNLLTKEASRT 147

Query: 162 AHYLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEFKENIVIDPGSVDIFPPLCMPNKL 221
           AH+LR+DFER GIHL  E+LD+VN L+  I QLC+EF ENI  DPG VDIFP   +P  L
Sbjct: 148 AHHLRMDFERGGIHLDPEKLDKVNNLTTNIFQLCREFSENIADDPGHVDIFPGSRIPRHL 207

Query: 222 HHLVKPIYRSSD--SFGSRKG---MKENGFRLVTDADTLYSVLQYASDDEVRKMAYIKGN 281
           HHL+ P YRS+   S GS +     K+ GFR+ TD  T+ S+LQ+ SD+EVRKM YI+GN
Sbjct: 208 HHLLNPTYRSTSGGSRGSTRSAHKSKQKGFRINTDPRTVSSILQWTSDEEVRKMVYIQGN 267

Query: 282 SSPRANLDVLDKLIATRHELAQIVGYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCA 341
           S P AN  VL+KLIA RHEL+Q++G  SYA+  V PNLA SP VV SFLQELSK V+P A
Sbjct: 268 SVPHANHGVLEKLIAARHELSQMMGCNSYADIMVEPNLAKSPKVVTSFLQELSKTVKPKA 327

Query: 342 DEEFNQIREFKLKKCVNKSEDLEPWDEAYYTSMMKSTAYNLDSS-ATSYFPLSQCIEGLK 401
           DEEF  IR+FK +KC N S +LEPWDE YYTSMMKS+  ++D++   SYFPL QCIEGLK
Sbjct: 328 DEEFIAIRDFKREKCGNPSAELEPWDETYYTSMMKSSINDVDTAVVASYFPLPQCIEGLK 387

Query: 402 ILVESLFGASFYSIPLAPGESWHPDVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFA 461
           +LVESLFGA+F++IPLAPGESWHP+V+KLSLHHP+EGDLGYLYLDLYSRKGKYPGCA FA
Sbjct: 388 VLVESLFGATFHTIPLAPGESWHPNVVKLSLHHPDEGDLGYLYLDLYSRKGKYPGCASFA 447

Query: 462 IRGGRKVSEMEYQLPVVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQ 521
           IRGGRK+SE EYQLPV+ALVCNFS +   S V+LNHSEVE LFHEFGHALHSLLSRT+YQ
Sbjct: 448 IRGGRKISETEYQLPVIALVCNFSRACDSSIVKLNHSEVEVLFHEFGHALHSLLSRTDYQ 507

Query: 522 HFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEAT 581
           HFSGTRV LDLAE PSNLFEYYA DYR+L+ FA+HYSTG+ IPEKLV S+QGA+ MF AT
Sbjct: 508 HFSGTRVALDLAEMPSNLFEYYAWDYRLLKRFARHYSTGETIPEKLVNSLQGARNMFAAT 567

Query: 582 ELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQYTNWKHVDDTHWQTRFCHLLTYGA 641
           E+QRQ+ YALIDQ LFGE+  + RD S +VAELKRQ+T+W HV+ THW  RF HLL YGA
Sbjct: 568 EMQRQVFYALIDQMLFGEQPETARDVSHLVAELKRQHTSWNHVEGTHWYIRFSHLLNYGA 627

Query: 642 GYYSYLYAKCFSATIWEKLCQEDPLSRNTGTALRTKFLQHGGSKEAVDLLTDLVGDGIVR 701
           GYYSYLYAKCF++TIW+ +C+EDPLS NTGT LR KF +HGG+K+  +LLTDL G  I+ 
Sbjct: 628 GYYSYLYAKCFASTIWQSICEEDPLSLNTGTLLREKFFKHGGAKDPAELLTDLAGKEIIS 687

Query: 702 YCDGGVIPDLTSLCKEMDL 715
               G++P  T L  E+ L
Sbjct: 688 VHGEGIVPATTYLLNELRL 706

BLAST of MC10g0202 vs. ExPASy Swiss-Prot
Match: Q5RF14 (Mitochondrial intermediate peptidase OS=Pongo abelii OX=9601 GN=MIPEP PE=2 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 5.6e-106
Identity = 231/677 (34.12%), Postives = 355/677 (52.44%), Query Frame = 0

Query: 42  GAATGLYGFEHLKSPKGFRRFVDEAIERSGELVTFISSKPSSAEVIRAMDEISNRVCSVF 101
           G   GL+G   L +P+GF    ++A+ ++  LV    S P   + +   DE+S+ +C V 
Sbjct: 60  GERRGLFGVPELSAPEGFHAAQEKALRKAELLVGRACSTPPGPQTVLIFDELSDSLCRVA 119

Query: 102 DSAELCRQTHPDREFVEEANKAAMRLNEYFHYLNTNHTLYNAVKKAEHEAHL---LTREA 161
           D A+  +  HP+  F E A +A   +      LNTN  LY +++K   +  L   L  E 
Sbjct: 120 DLADFVKIAHPEPAFREAAEEACRSIGTMVEKLNTNVDLYQSLRKLLADKKLVDSLDPET 179

Query: 162 HKAAHYLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEFKENIVIDPGSVDIFPPLCMP 221
            + A     DFE +GIHL  E+  R   L+++I  L   F                   P
Sbjct: 180 RRVAELFMFDFEISGIHLDKEKRKRAVDLNVKILDLSSTF-------------LMGANFP 239

Query: 222 NKLHHLVKPIYRSSDSFGSRKGMKENGFRLVTDADTLYSVLQYASDDEVRKMAYIKGNSS 281
           NK+   + P +        R+     G  ++ D      +   + DD VR+ AY      
Sbjct: 240 NKIEKHLLPEH-------IRRNFTSAGDHIIID-----GLHAESPDDLVREAAYKIFLYP 299

Query: 282 PRANLDVLDKLIATRHELAQIVGYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCADE 341
               L  L++L+++R  LA++VGY +++  A+   +A +P  VM FL++LS  +     +
Sbjct: 300 NAGQLKCLEELLSSRDLLAKLVGYSTFSHRALQGTIAKNPETVMQFLEKLSDKLSERTLK 359

Query: 342 EFNQIREFKLKKCVNKSEDLEPWDEAYYTSMMKSTAYNLDSSA-TSYFPLSQCIEGLKIL 401
           +F  IR  K+K     SE + PWD  YY+ ++++  YN++ S    +F L  C+EGL IL
Sbjct: 360 DFEMIRGMKMKLNPQNSE-VMPWDPPYYSGVIRAERYNIEPSLYCPFFSLGACMEGLNIL 419

Query: 402 VESLFGASFYSIPLAPGESWHPDVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIR 461
           +  L G S Y+   A GE W  DV KL++ H  EG LGY+Y D + R  K     HF IR
Sbjct: 420 LNRLLGISLYAEQPAKGEVWSEDVRKLAVVHESEGLLGYIYCDFFQRADKPHQDCHFTIR 479

Query: 462 GGRKVSEMEYQLPVVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHF 521
           GGR   + +YQLPVV L+ N   S   S   L    +E LFHE GHA+HS+L RT YQH 
Sbjct: 480 GGRLKEDGDYQLPVVVLMLNLPRSSRSSPTLLTPGMMENLFHEMGHAMHSMLGRTRYQHV 539

Query: 522 SGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEATEL 581
           +GTR   D AE PS L EY+A+DYRV+  FA+HY TG  +P+ +V  +  +KK+  A ++
Sbjct: 540 TGTRCPTDFAEVPSILMEYFANDYRVVNQFARHYQTGQPLPKNMVSRLCESKKVCAAADM 599

Query: 582 QRQILYALIDQTLFGERLTSERDTSSVVAELKRQYTNWKHVDDTHWQTRFCHLLTYGAGY 641
           Q Q+ YA +DQ   G+       T+ ++ E + ++    +V DT WQ RF HL+ YGA Y
Sbjct: 600 QLQVFYATLDQIYHGKH-PLRNSTTDILKETQEKFYGLPYVPDTAWQLRFSHLVGYGAKY 659

Query: 642 YSYLYAKCFSATIWEKLCQEDPLSRNTGTALRTKFLQHGGSKEAVDLLTDLVGDGIVRYC 701
           YSYL ++  ++ +W++   +DP +R  G   R + L HGG +E +     L+ +G+++ C
Sbjct: 660 YSYLMSRAVASMVWKECFLQDPFNRAAGERYRREMLAHGGGREPM-----LMVEGMLQKC 703

Query: 702 DGGVIPDLTSLCKEMDL 715
              V   +++L  ++DL
Sbjct: 720 P-SVDDFVSALVSDLDL 703

BLAST of MC10g0202 vs. ExPASy Swiss-Prot
Match: Q99797 (Mitochondrial intermediate peptidase OS=Homo sapiens OX=9606 GN=MIPEP PE=1 SV=2)

HSP 1 Score: 385.6 bits (989), Expect = 1.3e-105
Identity = 231/677 (34.12%), Postives = 356/677 (52.58%), Query Frame = 0

Query: 42  GAATGLYGFEHLKSPKGFRRFVDEAIERSGELVTFISSKPSSAEVIRAMDEISNRVCSVF 101
           G   GL+G   L +P+GF    ++A+ ++  LV    S P   + +   DE+S+ +C V 
Sbjct: 60  GERRGLFGVPELSAPEGFHIAQEKALRKTELLVDRACSTPPGPQTVLIFDELSDSLCRVA 119

Query: 102 DSAELCRQTHPDREFVEEANKAAMRLNEYFHYLNTNHTLYNAVKKAEHEAHL---LTREA 161
           D A+  +  HP+  F E A +A   +      LNTN  LY +++K   +  L   L  E 
Sbjct: 120 DLADFVKIAHPEPAFREAAEEACRSIGTMVEKLNTNVDLYQSLQKLLADKKLVDSLDPET 179

Query: 162 HKAAHYLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEFKENIVIDPGSVDIFPPLCMP 221
            + A     DFE +GIHL  E+  R   L+++I  L   F                   P
Sbjct: 180 RRVAELFMFDFEISGIHLDKEKRKRAVDLNVKILDLSSTF-------------LMGTNFP 239

Query: 222 NKLHHLVKPIYRSSDSFGSRKGMKENGFRLVTDADTLYSVLQYASDDEVRKMAYIKGNSS 281
           NK+   + P +        R+     G  ++ D      +   + DD VR+ AY      
Sbjct: 240 NKIEKHLLPEH-------IRRNFTSAGDHIIID-----GLHAESPDDLVREAAYKIFLYP 299

Query: 282 PRANLDVLDKLIATRHELAQIVGYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCADE 341
               L  L++L+++R  LA++VGY +++  A+   +A +P  VM FL++LS  +     +
Sbjct: 300 NAGQLKCLEELLSSRDLLAKLVGYSTFSHRALQGTIAKNPETVMQFLEKLSDKLSERTLK 359

Query: 342 EFNQIREFKLKKCVNKSEDLEPWDEAYYTSMMKSTAYNLDSSA-TSYFPLSQCIEGLKIL 401
           +F  IR  K+K     SE + PWD  YY+ ++++  YN++ S    +F L  C+EGL IL
Sbjct: 360 DFEMIRGMKMKLNPQNSE-VMPWDPPYYSGVIRAERYNIEPSLYCPFFSLGACMEGLNIL 419

Query: 402 VESLFGASFYSIPLAPGESWHPDVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIR 461
           +  L G S Y+   A GE W  DV KL++ H  EG LGY+Y D + R  K     HF IR
Sbjct: 420 LNRLLGISLYAEQPAKGEVWSEDVRKLAVVHESEGLLGYIYCDFFQRADKPHQDCHFTIR 479

Query: 462 GGRKVSEMEYQLPVVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHF 521
           GGR   + +YQLPVV L+ N   S   S   L  S +E LFHE GHA+HS+L RT YQH 
Sbjct: 480 GGRLKEDGDYQLPVVVLMLNLPRSSRSSPTLLTPSMMENLFHEMGHAMHSMLGRTRYQHV 539

Query: 522 SGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEATEL 581
           +GTR   D AE PS L EY+A+DYRV+  FA+HY TG  +P+ +V  +  +KK+  A ++
Sbjct: 540 TGTRCPTDFAEVPSILMEYFANDYRVVNQFARHYQTGQPLPKNMVSRLCESKKVCAAADM 599

Query: 582 QRQILYALIDQTLFGERLTSERDTSSVVAELKRQYTNWKHVDDTHWQTRFCHLLTYGAGY 641
           Q Q+ YA +DQ   G+       T+ ++ E + ++    +V +T WQ RF HL+ YGA Y
Sbjct: 600 QLQVFYATLDQIYHGKH-PLRNSTTDILKETQEKFYGLPYVPNTAWQLRFSHLVGYGARY 659

Query: 642 YSYLYAKCFSATIWEKLCQEDPLSRNTGTALRTKFLQHGGSKEAVDLLTDLVGDGIVRYC 701
           YSYL ++  ++ +W++   +DP +R  G   R + L HGG +E +     L+ +G+++ C
Sbjct: 660 YSYLMSRAVASMVWKECFLQDPFNRAAGERYRREMLAHGGGREPM-----LMVEGMLQKC 703

Query: 702 DGGVIPDLTSLCKEMDL 715
              V   +++L  ++DL
Sbjct: 720 P-SVDDFVSALVSDLDL 703

BLAST of MC10g0202 vs. ExPASy Swiss-Prot
Match: A6H611 (Mitochondrial intermediate peptidase OS=Mus musculus OX=10090 GN=Mipep PE=1 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 5.8e-103
Identity = 225/653 (34.46%), Postives = 346/653 (52.99%), Query Frame = 0

Query: 42  GAATGLYGFEHLKSPKGFRRFVDEAIERSGELVTFISSKPSSAEVIRAMDEISNRVCSVF 101
           G   GL+G   L +P+GF+   +EA++++  LV    S P   + +   DE+S+ +C V 
Sbjct: 58  GERRGLFGVPELSTPEGFQVAQEEALKKTEWLVERACSTPPGPQTVLIFDELSDCLCRVA 117

Query: 102 DSAELCRQTHPDREFVEEANKAAMRLNEYFHYLNTNHTLYNAVKKAEHEAHL---LTREA 161
           D A+  +  HPD  F E A +A   +      LNTN  LY ++++   +  L   L  E 
Sbjct: 118 DLADFVKIGHPDPAFREAAQEACRSIGTMVEKLNTNVELYQSLQRLLGDKKLMESLDAET 177

Query: 162 HKAAHYLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEFKENIVIDPGSVDIFPPLCMP 221
            + A     DFE +GIHL  E+  R   L+++I  L   F                   P
Sbjct: 178 RRVAELFMFDFEISGIHLDEEKRRRAVDLNVKILDLSNAF-------------LMRTNFP 237

Query: 222 NKLHHLVKPIYRSSDSFGSRKGMKENGFRLVTDADTLYSVLQYASDDEVRKMAYIKGNSS 281
           NK+   + P +        +     +G  L+ D      +   ASDD VR+ AY K    
Sbjct: 238 NKIRKSLLPEH-------IQHHFARDGSHLIID-----GLHAEASDDLVREAAY-KIFLY 297

Query: 282 PRAN-LDVLDKLIATRHELAQIVGYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCAD 341
           P A+ L  L++L+++R  LA++VGY +++  A+   +A +P  VM FL++LS+ +     
Sbjct: 298 PNADQLKCLEELLSSRDLLAKLVGYSTFSHRALQGTIAQTPETVMQFLEKLSEKLSERTR 357

Query: 342 EEFNQIREFKLKKCVNKSEDLEPWDEAYYTSMMKSTAYNLDSSA-TSYFPLSQCIEGLKI 401
           ++F  ++  K K     S+ L PWD  YY+ ++++  YN++ S    +  L  C+EGL +
Sbjct: 358 KDFKMMQGMKTKLNPQNSK-LMPWDPPYYSGVIRAERYNIEPSLYCPFLSLGACMEGLNV 417

Query: 402 LVESLFGASFYSIPLAPGESWHPDVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAI 461
           L   L G + Y+     GE W  D+ KL++ H  EG LGY+Y D + R  K     HF I
Sbjct: 418 LFNKLLGITLYAEQTFKGEVWCNDIRKLAVVHESEGLLGYIYCDFFQRANKPQQDCHFTI 477

Query: 462 RGGRKVSEMEYQLPVVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQH 521
           RGGR   +  YQLPVV L+ N   +       L    +E LFHE GHA+HS+L RT YQH
Sbjct: 478 RGGRLKEDGSYQLPVVVLMLNLPHASRDFPTLLTPGMMENLFHEMGHAMHSMLGRTRYQH 537

Query: 522 FSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEATE 581
            +GTR   D AE PS L EY+++DYRV+  FAKHY TG  +P+ +V  +  +KK+  A E
Sbjct: 538 VTGTRCPTDFAEVPSILMEYFSNDYRVVSQFAKHYQTGQPLPKAMVSRLCESKKVCTAAE 597

Query: 582 LQRQILYALIDQTLFGERLTSERDTSSVVAELKRQYTNWKHVDDTHWQTRFCHLLTYGAG 641
           +Q Q+ YA +DQ   G+    ++ T+ ++ E + Q+    +V DT WQ RF HL+ YGA 
Sbjct: 598 MQLQVFYAALDQIYHGQH-PLKKSTTDILMETQEQFYGLPYVPDTAWQLRFSHLVGYGAK 657

Query: 642 YYSYLYAKCFSATIWEKLCQEDPLSRNTGTALRTKFLQHGGSKEAVDLLTDLV 690
           YYSYL ++  ++ IW++   +DP +R  G   R + L HGG KE + ++  ++
Sbjct: 658 YYSYLMSRAVASMIWKECFLQDPFNRAAGERYRREMLAHGGGKEPMLMIQGML 682

BLAST of MC10g0202 vs. ExPASy Swiss-Prot
Match: Q01992 (Mitochondrial intermediate peptidase OS=Rattus norvegicus OX=10116 GN=Mipep PE=1 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 3.5e-100
Identity = 226/653 (34.61%), Postives = 342/653 (52.37%), Query Frame = 0

Query: 42  GAATGLYGFEHLKSPKGFRRFVDEAIERSGELVTFISSKPSSAEVIRAMDEISNRVCSVF 101
           G   GL+G   L +P+GF+   +EA+ ++  LV    S P   + +   DE+S+ +C V 
Sbjct: 58  GERRGLFGVPELSTPEGFQVAQEEALRKTEWLVERACSTPPGPQTVLIFDELSDCLCRVA 117

Query: 102 DSAELCRQTHPDREFVEEANKAAMRLNEYFHYLNTNHTLYNAVKKAEHEAHL---LTREA 161
           D A+  +  HP++ F E A +A   +      LNTN  LY +++K   +  L   L  E 
Sbjct: 118 DLADFVKIGHPEQAFREAAQEACRSIGTMVEKLNTNVELYQSLQKLLDDKKLMDSLDAET 177

Query: 162 HKAAHYLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEFKENIVIDPGSVDIFPPLCMP 221
            + A     DFE +GIHL  E+  R   L+++I  L   F   ++     + I   L   
Sbjct: 178 RRVAELFMFDFEISGIHLDEEKRRRAVDLNVKILDLSSAF---LMGTNFPIKIQKHLLPE 237

Query: 222 NKLHHLVKPIYRSSDSFGSRKGMKENGFRLVTDADTLYSVLQYASDDEVRKMAYIKGNSS 281
           +  HH  +                 +G  LV D      +   ASDD VR+ AY K    
Sbjct: 238 HIQHHFAR-----------------DGRHLVID-----GLHAEASDDLVREAAY-KIFLY 297

Query: 282 PRAN-LDVLDKLIATRHELAQIVGYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCAD 341
           P A+ L  L++L+++R  LA +VGY  +        +A +P  VM FL++LS+ +     
Sbjct: 298 PNADQLKCLEELLSSRDLLANLVGYLPFPT-GPPGTIAQTPETVMQFLEKLSEKLCERTR 357

Query: 342 EEFNQIREFKLKKCVNKSEDLEPWDEAYYTSMMKSTAYNLDSSA-TSYFPLSQCIEGLKI 401
           ++F  ++  K K     SE L PWD  YY+ ++++  YN++ S    +  L  C+EGL +
Sbjct: 358 KDFEMMQGMKTKLNPQNSE-LMPWDPPYYSGVIRAERYNIEPSLYCPFLSLGACMEGLNV 417

Query: 402 LVESLFGASFYSIPLAPGESWHPDVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAI 461
           L   L G + Y+     GE W  DV KL++ H  EG LGY+Y D + R  K     HF I
Sbjct: 418 LFNRLLGVTLYAEQPFKGEVWCIDVRKLAVVHESEGLLGYIYCDFFQRANKPQQDCHFTI 477

Query: 462 RGGRKVSEMEYQLPVVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQH 521
           RGGR   +  YQLPVV L+ N   +       L    +E LFHE GHA+HS+L RT YQH
Sbjct: 478 RGGRLKEDGSYQLPVVVLMLNLPHASRDFPTLLTPGMMENLFHEMGHAMHSMLGRTRYQH 537

Query: 522 FSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEATE 581
            +GTR   D AE PS L EY+++DYRV+  FAKHY TG  +P+ +V  +  +KK+  A E
Sbjct: 538 VTGTRCPTDFAEVPSILMEYFSNDYRVVSQFAKHYQTGQPLPKAMVSRLCESKKVCAAAE 597

Query: 582 LQRQILYALIDQTLFGERLTSERDTSSVVAELKRQYTNWKHVDDTHWQTRFCHLLTYGAG 641
           +Q Q+ YA +DQ   G+    ++ T+ ++ E + Q+    +V DT WQ RF HL+ YGA 
Sbjct: 598 MQLQVFYAALDQIYHGQH-PLKKSTTDILMETQEQFYGLPYVPDTAWQLRFSHLVGYGAK 657

Query: 642 YYSYLYAKCFSATIWEKLCQEDPLSRNTGTALRTKFLQHGGSKEAVDLLTDLV 690
           YYSYL ++  ++ +W++   +DP +R  G   R + L HGG KE + ++  ++
Sbjct: 658 YYSYLMSRAVASMVWKECFLQDPFNRAAGERYRREMLAHGGGKEPMLMIQGML 681

BLAST of MC10g0202 vs. NCBI nr
Match: XP_022141120.1 (probable mitochondrial intermediate peptidase, mitochondrial, partial [Momordica charantia])

HSP 1 Score: 1379 bits (3568), Expect = 0.0
Identity = 686/689 (99.56%), Postives = 686/689 (99.56%), Query Frame = 0

Query: 31  SIHAPASAPQKGAATGLYGFEHLKSPKGFRRFVDEAIERSGELVTFISSKPSSAEVIRAM 90
           SIHAPASAPQKGAATGLYGFEHLKSPKGFRRFVDEAIERSGELVTFISSKPSSAEVIRAM
Sbjct: 1   SIHAPASAPQKGAATGLYGFEHLKSPKGFRRFVDEAIERSGELVTFISSKPSSAEVIRAM 60

Query: 91  DEISNRVCSVFDSAELCRQTHPDREFVEEANKAAMRLNEYFHYLNTNHTLYNAVKKAEHE 150
           DEISNRVCSVFDSAELCRQTHPDREFVEEANKAAMRLNEYFHYLNTNHTLYNAVKKAEHE
Sbjct: 61  DEISNRVCSVFDSAELCRQTHPDREFVEEANKAAMRLNEYFHYLNTNHTLYNAVKKAEHE 120

Query: 151 AHLLTREAHKAAHYLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEFKENIVIDPGSVD 210
           AHLLTREAHKAAHYLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEFKENIVIDPGSVD
Sbjct: 121 AHLLTREAHKAAHYLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEFKENIVIDPGSVD 180

Query: 211 IFPPLCMPNKLHHLVKPIYRSSDSFGSRKGMKENGFRLVTDADTLYSVLQYASDDEVRKM 270
           IFPPLCMPNKLHHLVKPIYRSSDSFGSRKGMKENGFRLVTDADTLYSVLQYASDDEVRKM
Sbjct: 181 IFPPLCMPNKLHHLVKPIYRSSDSFGSRKGMKENGFRLVTDADTLYSVLQYASDDEVRKM 240

Query: 271 AYIKGNSSPRANLDVLDKLIATRHELAQIVGYRSYAEFAVTPNLASSPAVVMSFLQELSK 330
           AYIKGNSSPRANLDVLDKLIATRHELAQIVGYRSYAEFAVTPNLASSPAVVMSFLQELSK
Sbjct: 241 AYIKGNSSPRANLDVLDKLIATRHELAQIVGYRSYAEFAVTPNLASSPAVVMSFLQELSK 300

Query: 331 VVRPCADEEFNQIREFKLKKCVNKSEDLEPWDEAYYTSMMKSTAYNLDSSAT-SYFPLSQ 390
           VVRPCADEEFNQIREFKLKKCVNKSEDLEPWDEAYYTSMMKSTAYNLDSS   SYFPLSQ
Sbjct: 301 VVRPCADEEFNQIREFKLKKCVNKSEDLEPWDEAYYTSMMKSTAYNLDSSVVASYFPLSQ 360

Query: 391 CIEGLKILVESLFGASFYSIPLAPGESWHPDVLKLSLHHPEEGDLGYLYLDLYSRKGKYP 450
           CIEGLKILVESLFGASFYSIPLAPGESWHPDVLKLSLHHPEEGDLGYLYLDLYSRKGKYP
Sbjct: 361 CIEGLKILVESLFGASFYSIPLAPGESWHPDVLKLSLHHPEEGDLGYLYLDLYSRKGKYP 420

Query: 451 GCAHFAIRGGRKVSEMEYQLPVVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALHSLL 510
           GCAHFAIRGGRKVSEMEYQLPVVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALHSLL
Sbjct: 421 GCAHFAIRGGRKVSEMEYQLPVVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALHSLL 480

Query: 511 SRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAK 570
           SRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAK
Sbjct: 481 SRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAK 540

Query: 571 KMFEATELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQYTNWKHVDDTHWQTRFCH 630
           KMFEATELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQYTNWKHVDDTHWQTRFCH
Sbjct: 541 KMFEATELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQYTNWKHVDDTHWQTRFCH 600

Query: 631 LLTYGAGYYSYLYAKCFSATIWEKLCQEDPLSRNTGTALRTKFLQHGGSKEAVDLLTDLV 690
           LLTYGAGYYSYLYAKCFSATIWEKLCQEDPLSRNTGTALRTKFLQHGGSKEAVDLLTDLV
Sbjct: 601 LLTYGAGYYSYLYAKCFSATIWEKLCQEDPLSRNTGTALRTKFLQHGGSKEAVDLLTDLV 660

Query: 691 GDGIVRYCDGGVIPDLTSLCKEMDLSGSL 718
           GDGIVRYCDGGVIPDLTSLCKEMDLSGSL
Sbjct: 661 GDGIVRYCDGGVIPDLTSLCKEMDLSGSL 689

BLAST of MC10g0202 vs. NCBI nr
Match: XP_022941716.1 (mitochondrial intermediate peptidase, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 1277 bits (3305), Expect = 0.0
Identity = 642/719 (89.29%), Postives = 674/719 (93.74%), Query Frame = 0

Query: 1   MSSLIRRTTPKLSSGAALKSCFSNRHRSRRSIHAPASAPQKGAATGLYGFEHLKSPKGFR 60
           M SLIR+TT K+SSGA LKSC+ N   S RSIHA ASAP +GAATGLYGFE LKSPKGFR
Sbjct: 1   MLSLIRKTTRKVSSGAVLKSCYLN-CLSSRSIHAAASAPHEGAATGLYGFEQLKSPKGFR 60

Query: 61  RFVDEAIERSGELVTFISSKPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA 120
           RFVDEAIERSGELVTFISS PSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA
Sbjct: 61  RFVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA 120

Query: 121 NKAAMRLNEYFHYLNTNHTLYNAVKKAEHEAHLLTREAHKAAHYLRVDFERAGIHLSAER 180
           NKAAMR++EYFH+LNTNHTLYNAVKKAE EAHLLTREAH AAH+LRVDFERAGIHLSAER
Sbjct: 121 NKAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAER 180

Query: 181 LDRVNQLSIEISQLCQEFKENIVIDPGSVDIFPPLCMPNKLHHLVKPIYRSSDSFGSRKG 240
           LDRVNQLSIEISQLCQE+KENIVIDPGSVDIFPP+ +PN LHHLVKPIYRSS SFGS   
Sbjct: 181 LDRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISS 240

Query: 241 MKENGFRLVTDADTLYSVLQYASDDEVRKMAYIKGNSSPRANLDVLDKLIATRHELAQIV 300
           MKE GFRL+TDAD+L S+LQY SDDEVRKMAY+KGNSSP ANLDVLDK+IATRHELAQI+
Sbjct: 241 MKEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQIL 300

Query: 301 GYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCADEEFNQIREFKLKKCVNKSEDLEP 360
           GYRSYAEFAVTPN+ASSPAVV+SFLQELS +VRP ADEEFNQI+EFKLKK  NK EDLEP
Sbjct: 301 GYRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIKEFKLKKRFNKFEDLEP 360

Query: 361 WDEAYYTSMMKSTAYNLDSSA-TSYFPLSQCIEGLKILVESLFGASFYSIPLAPGESWHP 420
           WDEAYYTSMMKSTAYNL+SS  +SYFPLSQCIEGLK LV SLFGASF SIPLAPGESWHP
Sbjct: 361 WDEAYYTSMMKSTAYNLNSSVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHP 420

Query: 421 DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIRGGRKVSEMEYQLPVVALVCNFS 480
           DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAI+GG KVSEMEYQLPVVALVCNFS
Sbjct: 421 DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFS 480

Query: 481 SSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS 540
            S  RSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS
Sbjct: 481 GSRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS 540

Query: 541 DYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEATELQRQILYALIDQTLFGERLTSER 600
           DYRVLRTFAKHYSTG++IPEKLVKSMQGAKKMF ATELQRQILYALIDQTLFGERLTSER
Sbjct: 541 DYRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSER 600

Query: 601 DTSSVVAELKRQYTNWKHVDDTHWQTRFCHLLTYGAGYYSYLYAKCFSATIWEKLCQEDP 660
           DTSSVVAELKRQ T+WKHVD THWQTRF HLLTYGAGYYSYLYAKCF+ATIWEKLCQEDP
Sbjct: 601 DTSSVVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDP 660

Query: 661 LSRNTGTALRTKFLQHGGSKEAVDLLTDLVGDGIVRYCDGGVIPDLTSLCKEMDLSGSL 718
           LSR+TGTALRTKFLQHGGSKE VDLL DLVGDGI+RYCDGGVIPD+TSLCKEMD+SG L
Sbjct: 661 LSRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKL 718

BLAST of MC10g0202 vs. NCBI nr
Match: XP_022972575.1 (mitochondrial intermediate peptidase, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 1275 bits (3300), Expect = 0.0
Identity = 640/719 (89.01%), Postives = 674/719 (93.74%), Query Frame = 0

Query: 1   MSSLIRRTTPKLSSGAALKSCFSNRHRSRRSIHAPASAPQKGAATGLYGFEHLKSPKGFR 60
           M SLIR+TT K+SSGA LKSC+ N   S RSIHA ASAP +GAATGLYGFE LKSPKGF+
Sbjct: 1   MLSLIRKTTRKVSSGAVLKSCYLN-CLSSRSIHAAASAPHEGAATGLYGFEQLKSPKGFQ 60

Query: 61  RFVDEAIERSGELVTFISSKPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA 120
           RFVDEAIERSGELVTFISS PSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA
Sbjct: 61  RFVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA 120

Query: 121 NKAAMRLNEYFHYLNTNHTLYNAVKKAEHEAHLLTREAHKAAHYLRVDFERAGIHLSAER 180
           NKAAMR++EYFH+LNTNHTLYNAVKKAEHEAHLLTREAH AAH+LRVDFERAGIHLSAER
Sbjct: 121 NKAAMRMSEYFHFLNTNHTLYNAVKKAEHEAHLLTREAHMAAHFLRVDFERAGIHLSAER 180

Query: 181 LDRVNQLSIEISQLCQEFKENIVIDPGSVDIFPPLCMPNKLHHLVKPIYRSSDSFGSRKG 240
           LDRVNQLSIEISQLCQE+KENIVIDPGSVDIFPP+ +PN LHHLVKPIYRSS SFGS   
Sbjct: 181 LDRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISS 240

Query: 241 MKENGFRLVTDADTLYSVLQYASDDEVRKMAYIKGNSSPRANLDVLDKLIATRHELAQIV 300
           MKE GFRL+TDAD+L S+LQY SDDEVRKMAY+KGNSSPRANLDVLDK+IATRHELAQI+
Sbjct: 241 MKEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPRANLDVLDKIIATRHELAQIL 300

Query: 301 GYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCADEEFNQIREFKLKKCVNKSEDLEP 360
           GYRSYAEFAVTPN+ASSPAVV+SFLQELS +VRP ADEEFNQIREFKLKK  NK EDLEP
Sbjct: 301 GYRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEP 360

Query: 361 WDEAYYTSMMKSTAYNLDSSA-TSYFPLSQCIEGLKILVESLFGASFYSIPLAPGESWHP 420
           WDEAYYTSMMKSTAYNL+SS  +SYFPL+QCIEGLK LV SLFGASF SI LAPGESWHP
Sbjct: 361 WDEAYYTSMMKSTAYNLNSSVISSYFPLTQCIEGLKTLVNSLFGASFNSISLAPGESWHP 420

Query: 421 DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIRGGRKVSEMEYQLPVVALVCNFS 480
           DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAI+GG KVSEMEYQLPVVALVCNFS
Sbjct: 421 DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFS 480

Query: 481 SSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS 540
            S  RSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS
Sbjct: 481 GSRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS 540

Query: 541 DYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEATELQRQILYALIDQTLFGERLTSER 600
           DYRVLRTFAKHYSTG++IPEKLVKSMQGAKKMF ATELQRQILYALIDQTLFGERLT ER
Sbjct: 541 DYRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTLER 600

Query: 601 DTSSVVAELKRQYTNWKHVDDTHWQTRFCHLLTYGAGYYSYLYAKCFSATIWEKLCQEDP 660
           DTSSVVAELKRQ T+WKHVD THWQTRF HLLTYGAGYYSYLYAKCF+ATIWEKLCQEDP
Sbjct: 601 DTSSVVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDP 660

Query: 661 LSRNTGTALRTKFLQHGGSKEAVDLLTDLVGDGIVRYCDGGVIPDLTSLCKEMDLSGSL 718
           LSR+TGTALRTKFLQHGGSKE VDLL DL+GDGI+RYCDGGVIPD+TSLCKEMD+SG L
Sbjct: 661 LSRSTGTALRTKFLQHGGSKEPVDLLKDLLGDGIIRYCDGGVIPDITSLCKEMDISGKL 718

BLAST of MC10g0202 vs. NCBI nr
Match: XP_023518581.1 (mitochondrial intermediate peptidase, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1274 bits (3297), Expect = 0.0
Identity = 640/719 (89.01%), Postives = 673/719 (93.60%), Query Frame = 0

Query: 1   MSSLIRRTTPKLSSGAALKSCFSNRHRSRRSIHAPASAPQKGAATGLYGFEHLKSPKGFR 60
           M SLIR+TT K+SSGA LKSC+ N   S RSIHA ASAP +GAATGLYGFE LKSPKGFR
Sbjct: 1   MLSLIRKTTRKVSSGAILKSCYLN-CLSSRSIHAAASAPHEGAATGLYGFEQLKSPKGFR 60

Query: 61  RFVDEAIERSGELVTFISSKPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA 120
           RFVDEAIERSGELVTFISS PSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA
Sbjct: 61  RFVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA 120

Query: 121 NKAAMRLNEYFHYLNTNHTLYNAVKKAEHEAHLLTREAHKAAHYLRVDFERAGIHLSAER 180
           NKAAMR++EYFH+LNTNHTLYNAVKKAE EAHLLT EAH AAH+LRVDFERAGIHLSAER
Sbjct: 121 NKAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTHEAHMAAHFLRVDFERAGIHLSAER 180

Query: 181 LDRVNQLSIEISQLCQEFKENIVIDPGSVDIFPPLCMPNKLHHLVKPIYRSSDSFGSRKG 240
           LDRVNQLSIEISQLCQE+KENIVIDPGSVDIFPP+ +PN LHHLVKPIYRSS SFGS   
Sbjct: 181 LDRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISS 240

Query: 241 MKENGFRLVTDADTLYSVLQYASDDEVRKMAYIKGNSSPRANLDVLDKLIATRHELAQIV 300
           MKE GFRL+TDAD+L S+LQY SDDEVRKMAY+KGNSSP ANLDVLDK+IATRHELAQI+
Sbjct: 241 MKEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPHANLDVLDKIIATRHELAQIL 300

Query: 301 GYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCADEEFNQIREFKLKKCVNKSEDLEP 360
           GY+SYAEFAVTPN+ASSPAVV+SFLQELS +VRP ADEEFNQIREFKLKK  NK EDLEP
Sbjct: 301 GYKSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEP 360

Query: 361 WDEAYYTSMMKSTAYNLDSSA-TSYFPLSQCIEGLKILVESLFGASFYSIPLAPGESWHP 420
           WDEAYYTSMMKSTAYNL+SS  +SYFPLSQCIEGLK LV SLFGASF SIPLAPGESWHP
Sbjct: 361 WDEAYYTSMMKSTAYNLNSSVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHP 420

Query: 421 DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIRGGRKVSEMEYQLPVVALVCNFS 480
           DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAI+GG KVSEMEYQLPVVALVCNF 
Sbjct: 421 DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFY 480

Query: 481 SSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS 540
            S  RSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS
Sbjct: 481 GSRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS 540

Query: 541 DYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEATELQRQILYALIDQTLFGERLTSER 600
           DYRVLRTFAKHYSTG++IPEKLVKSMQGAKKMF ATELQRQILYALIDQTLFGERLTSER
Sbjct: 541 DYRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSER 600

Query: 601 DTSSVVAELKRQYTNWKHVDDTHWQTRFCHLLTYGAGYYSYLYAKCFSATIWEKLCQEDP 660
           DTSSVVAELKRQ T+WKHVD THWQTRF HLLTYGAGYYSYLYAKCF+ATIWEKLCQEDP
Sbjct: 601 DTSSVVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDP 660

Query: 661 LSRNTGTALRTKFLQHGGSKEAVDLLTDLVGDGIVRYCDGGVIPDLTSLCKEMDLSGSL 718
           LSR+TGTALRTKFLQHGGSKE VDLL DLVGDGI+RYCDGGVIPD+TSLCKEMD+SG+L
Sbjct: 661 LSRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGNL 718

BLAST of MC10g0202 vs. NCBI nr
Match: XP_004148134.1 (mitochondrial intermediate peptidase, mitochondrial isoform X3 [Cucumis sativus] >KGN57283.1 hypothetical protein Csa_010120 [Cucumis sativus])

HSP 1 Score: 1269 bits (3285), Expect = 0.0
Identity = 632/719 (87.90%), Postives = 677/719 (94.16%), Query Frame = 0

Query: 1   MSSLIRRTTPKLSSGAALKSCFSNRHRSRRSIHAPASAPQKGAATGLYGFEHLKSPKGFR 60
           MSSLIR+T  KL+SG  LKS + N  RSR SIHAPASAPQKGAATGLYGF+HLKSP+GFR
Sbjct: 1   MSSLIRKTPGKLTSGLVLKSSYWNCFRSR-SIHAPASAPQKGAATGLYGFDHLKSPQGFR 60

Query: 61  RFVDEAIERSGELVTFISSKPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA 120
           RFVDEAIERSGELVTFISS PSSAEVIRAMDEISN VCSVFDSAELCRQTHPDREFVEEA
Sbjct: 61  RFVDEAIERSGELVTFISSMPSSAEVIRAMDEISNSVCSVFDSAELCRQTHPDREFVEEA 120

Query: 121 NKAAMRLNEYFHYLNTNHTLYNAVKKAEHEAHLLTREAHKAAHYLRVDFERAGIHLSAER 180
           N AAMR+NEYFH+LNTNHTLY+AVKKAEHEAHLLTREAH AAHYLRVDFERAGIHLSA++
Sbjct: 121 NNAAMRMNEYFHFLNTNHTLYSAVKKAEHEAHLLTREAHMAAHYLRVDFERAGIHLSADK 180

Query: 181 LDRVNQLSIEISQLCQEFKENIVIDPGSVDIFPPLCMPNKLHHLVKPIYRSSDSFGSRKG 240
           LDRVNQL+IEISQLCQEFKENI+IDPG VDIFPPL MPN LHHL KPIYRSS+SFGSR  
Sbjct: 181 LDRVNQLNIEISQLCQEFKENILIDPGYVDIFPPLRMPNNLHHLAKPIYRSSESFGSRSS 240

Query: 241 MKENGFRLVTDADTLYSVLQYASDDEVRKMAYIKGNSSPRANLDVLDKLIATRHELAQIV 300
            KENGFRL+TD+D+L S+LQ+ASDDEVRKMAY+KGNSSPRANL VLDKLIATRH LAQI+
Sbjct: 241 KKENGFRLMTDSDSLSSILQFASDDEVRKMAYVKGNSSPRANLGVLDKLIATRHSLAQIL 300

Query: 301 GYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCADEEFNQIREFKLKKCVNKSEDLEP 360
           GYRS+AEFAVTPNLASSPAVVMSFLQELSKVVR  ADEEFNQIREFKLKKC+NK EDLEP
Sbjct: 301 GYRSFAEFAVTPNLASSPAVVMSFLQELSKVVRSRADEEFNQIREFKLKKCINKFEDLEP 360

Query: 361 WDEAYYTSMMKSTAYNLDSSA-TSYFPLSQCIEGLKILVESLFGASFYSIPLAPGESWHP 420
           WDEAYYTSMMKSTAYNLDSS   SYFPLSQCIEGLK LV+SLFGASFY+IPLAPGESWHP
Sbjct: 361 WDEAYYTSMMKSTAYNLDSSVIASYFPLSQCIEGLKTLVKSLFGASFYNIPLAPGESWHP 420

Query: 421 DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIRGGRKVSEMEYQLPVVALVCNFS 480
           DVLKLSL HPEEG+LG+LYLDLYSRKGKYPGCAHFAIRGGRKVSE EYQLPVVALVCNFS
Sbjct: 421 DVLKLSLQHPEEGELGFLYLDLYSRKGKYPGCAHFAIRGGRKVSETEYQLPVVALVCNFS 480

Query: 481 SSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS 540
           SS+ RSNVRLNHSE+ETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS
Sbjct: 481 SSNDRSNVRLNHSELETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS 540

Query: 541 DYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEATELQRQILYALIDQTLFGERLTSER 600
           DYRVL+TFAKHYSTG+I+PEKLVKSM+GAK MF ATELQRQILYALIDQTLFGE+LTSER
Sbjct: 541 DYRVLKTFAKHYSTGEILPEKLVKSMKGAKMMFAATELQRQILYALIDQTLFGEKLTSER 600

Query: 601 DTSSVVAELKRQYTNWKHVDDTHWQTRFCHLLTYGAGYYSYLYAKCFSATIWEKLCQEDP 660
           DT SVVA+LKRQYT+WKHVD THWQ++FCHLLTYGAGYY+YLYAKCF+ATIWEKLC+EDP
Sbjct: 601 DTVSVVADLKRQYTSWKHVDGTHWQSQFCHLLTYGAGYYTYLYAKCFAATIWEKLCKEDP 660

Query: 661 LSRNTGTALRTKFLQHGGSKEAVDLLTDLVGDGIVRYCDGGVIPDLTSLCKEMDLSGSL 718
           LSR TG ALRTKFLQHGGSKEAVDLLTDLVGDGI+RY +GGVIPD+TSLCKEM L+ +L
Sbjct: 661 LSRETGNALRTKFLQHGGSKEAVDLLTDLVGDGIIRYSEGGVIPDITSLCKEMGLTKNL 718

BLAST of MC10g0202 vs. ExPASy TrEMBL
Match: A0A6J1CH32 (probable mitochondrial intermediate peptidase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011592 PE=3 SV=1)

HSP 1 Score: 1379 bits (3568), Expect = 0.0
Identity = 686/689 (99.56%), Postives = 686/689 (99.56%), Query Frame = 0

Query: 31  SIHAPASAPQKGAATGLYGFEHLKSPKGFRRFVDEAIERSGELVTFISSKPSSAEVIRAM 90
           SIHAPASAPQKGAATGLYGFEHLKSPKGFRRFVDEAIERSGELVTFISSKPSSAEVIRAM
Sbjct: 1   SIHAPASAPQKGAATGLYGFEHLKSPKGFRRFVDEAIERSGELVTFISSKPSSAEVIRAM 60

Query: 91  DEISNRVCSVFDSAELCRQTHPDREFVEEANKAAMRLNEYFHYLNTNHTLYNAVKKAEHE 150
           DEISNRVCSVFDSAELCRQTHPDREFVEEANKAAMRLNEYFHYLNTNHTLYNAVKKAEHE
Sbjct: 61  DEISNRVCSVFDSAELCRQTHPDREFVEEANKAAMRLNEYFHYLNTNHTLYNAVKKAEHE 120

Query: 151 AHLLTREAHKAAHYLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEFKENIVIDPGSVD 210
           AHLLTREAHKAAHYLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEFKENIVIDPGSVD
Sbjct: 121 AHLLTREAHKAAHYLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEFKENIVIDPGSVD 180

Query: 211 IFPPLCMPNKLHHLVKPIYRSSDSFGSRKGMKENGFRLVTDADTLYSVLQYASDDEVRKM 270
           IFPPLCMPNKLHHLVKPIYRSSDSFGSRKGMKENGFRLVTDADTLYSVLQYASDDEVRKM
Sbjct: 181 IFPPLCMPNKLHHLVKPIYRSSDSFGSRKGMKENGFRLVTDADTLYSVLQYASDDEVRKM 240

Query: 271 AYIKGNSSPRANLDVLDKLIATRHELAQIVGYRSYAEFAVTPNLASSPAVVMSFLQELSK 330
           AYIKGNSSPRANLDVLDKLIATRHELAQIVGYRSYAEFAVTPNLASSPAVVMSFLQELSK
Sbjct: 241 AYIKGNSSPRANLDVLDKLIATRHELAQIVGYRSYAEFAVTPNLASSPAVVMSFLQELSK 300

Query: 331 VVRPCADEEFNQIREFKLKKCVNKSEDLEPWDEAYYTSMMKSTAYNLDSSAT-SYFPLSQ 390
           VVRPCADEEFNQIREFKLKKCVNKSEDLEPWDEAYYTSMMKSTAYNLDSS   SYFPLSQ
Sbjct: 301 VVRPCADEEFNQIREFKLKKCVNKSEDLEPWDEAYYTSMMKSTAYNLDSSVVASYFPLSQ 360

Query: 391 CIEGLKILVESLFGASFYSIPLAPGESWHPDVLKLSLHHPEEGDLGYLYLDLYSRKGKYP 450
           CIEGLKILVESLFGASFYSIPLAPGESWHPDVLKLSLHHPEEGDLGYLYLDLYSRKGKYP
Sbjct: 361 CIEGLKILVESLFGASFYSIPLAPGESWHPDVLKLSLHHPEEGDLGYLYLDLYSRKGKYP 420

Query: 451 GCAHFAIRGGRKVSEMEYQLPVVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALHSLL 510
           GCAHFAIRGGRKVSEMEYQLPVVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALHSLL
Sbjct: 421 GCAHFAIRGGRKVSEMEYQLPVVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALHSLL 480

Query: 511 SRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAK 570
           SRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAK
Sbjct: 481 SRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAK 540

Query: 571 KMFEATELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQYTNWKHVDDTHWQTRFCH 630
           KMFEATELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQYTNWKHVDDTHWQTRFCH
Sbjct: 541 KMFEATELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQYTNWKHVDDTHWQTRFCH 600

Query: 631 LLTYGAGYYSYLYAKCFSATIWEKLCQEDPLSRNTGTALRTKFLQHGGSKEAVDLLTDLV 690
           LLTYGAGYYSYLYAKCFSATIWEKLCQEDPLSRNTGTALRTKFLQHGGSKEAVDLLTDLV
Sbjct: 601 LLTYGAGYYSYLYAKCFSATIWEKLCQEDPLSRNTGTALRTKFLQHGGSKEAVDLLTDLV 660

Query: 691 GDGIVRYCDGGVIPDLTSLCKEMDLSGSL 718
           GDGIVRYCDGGVIPDLTSLCKEMDLSGSL
Sbjct: 661 GDGIVRYCDGGVIPDLTSLCKEMDLSGSL 689

BLAST of MC10g0202 vs. ExPASy TrEMBL
Match: A0A6J1FLV9 (mitochondrial intermediate peptidase, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446997 PE=3 SV=1)

HSP 1 Score: 1277 bits (3305), Expect = 0.0
Identity = 642/719 (89.29%), Postives = 674/719 (93.74%), Query Frame = 0

Query: 1   MSSLIRRTTPKLSSGAALKSCFSNRHRSRRSIHAPASAPQKGAATGLYGFEHLKSPKGFR 60
           M SLIR+TT K+SSGA LKSC+ N   S RSIHA ASAP +GAATGLYGFE LKSPKGFR
Sbjct: 1   MLSLIRKTTRKVSSGAVLKSCYLN-CLSSRSIHAAASAPHEGAATGLYGFEQLKSPKGFR 60

Query: 61  RFVDEAIERSGELVTFISSKPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA 120
           RFVDEAIERSGELVTFISS PSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA
Sbjct: 61  RFVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA 120

Query: 121 NKAAMRLNEYFHYLNTNHTLYNAVKKAEHEAHLLTREAHKAAHYLRVDFERAGIHLSAER 180
           NKAAMR++EYFH+LNTNHTLYNAVKKAE EAHLLTREAH AAH+LRVDFERAGIHLSAER
Sbjct: 121 NKAAMRMSEYFHFLNTNHTLYNAVKKAEREAHLLTREAHMAAHFLRVDFERAGIHLSAER 180

Query: 181 LDRVNQLSIEISQLCQEFKENIVIDPGSVDIFPPLCMPNKLHHLVKPIYRSSDSFGSRKG 240
           LDRVNQLSIEISQLCQE+KENIVIDPGSVDIFPP+ +PN LHHLVKPIYRSS SFGS   
Sbjct: 181 LDRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISS 240

Query: 241 MKENGFRLVTDADTLYSVLQYASDDEVRKMAYIKGNSSPRANLDVLDKLIATRHELAQIV 300
           MKE GFRL+TDAD+L S+LQY SDDEVRKMAY+KGNSSP ANLDVLDK+IATRHELAQI+
Sbjct: 241 MKEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPCANLDVLDKIIATRHELAQIL 300

Query: 301 GYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCADEEFNQIREFKLKKCVNKSEDLEP 360
           GYRSYAEFAVTPN+ASSPAVV+SFLQELS +VRP ADEEFNQI+EFKLKK  NK EDLEP
Sbjct: 301 GYRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIKEFKLKKRFNKFEDLEP 360

Query: 361 WDEAYYTSMMKSTAYNLDSSA-TSYFPLSQCIEGLKILVESLFGASFYSIPLAPGESWHP 420
           WDEAYYTSMMKSTAYNL+SS  +SYFPLSQCIEGLK LV SLFGASF SIPLAPGESWHP
Sbjct: 361 WDEAYYTSMMKSTAYNLNSSVISSYFPLSQCIEGLKTLVNSLFGASFNSIPLAPGESWHP 420

Query: 421 DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIRGGRKVSEMEYQLPVVALVCNFS 480
           DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAI+GG KVSEMEYQLPVVALVCNFS
Sbjct: 421 DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFS 480

Query: 481 SSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS 540
            S  RSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS
Sbjct: 481 GSRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS 540

Query: 541 DYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEATELQRQILYALIDQTLFGERLTSER 600
           DYRVLRTFAKHYSTG++IPEKLVKSMQGAKKMF ATELQRQILYALIDQTLFGERLTSER
Sbjct: 541 DYRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTSER 600

Query: 601 DTSSVVAELKRQYTNWKHVDDTHWQTRFCHLLTYGAGYYSYLYAKCFSATIWEKLCQEDP 660
           DTSSVVAELKRQ T+WKHVD THWQTRF HLLTYGAGYYSYLYAKCF+ATIWEKLCQEDP
Sbjct: 601 DTSSVVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDP 660

Query: 661 LSRNTGTALRTKFLQHGGSKEAVDLLTDLVGDGIVRYCDGGVIPDLTSLCKEMDLSGSL 718
           LSR+TGTALRTKFLQHGGSKE VDLL DLVGDGI+RYCDGGVIPD+TSLCKEMD+SG L
Sbjct: 661 LSRSTGTALRTKFLQHGGSKEPVDLLKDLVGDGIIRYCDGGVIPDITSLCKEMDISGKL 718

BLAST of MC10g0202 vs. ExPASy TrEMBL
Match: A0A6J1I911 (mitochondrial intermediate peptidase, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471087 PE=3 SV=1)

HSP 1 Score: 1275 bits (3300), Expect = 0.0
Identity = 640/719 (89.01%), Postives = 674/719 (93.74%), Query Frame = 0

Query: 1   MSSLIRRTTPKLSSGAALKSCFSNRHRSRRSIHAPASAPQKGAATGLYGFEHLKSPKGFR 60
           M SLIR+TT K+SSGA LKSC+ N   S RSIHA ASAP +GAATGLYGFE LKSPKGF+
Sbjct: 1   MLSLIRKTTRKVSSGAVLKSCYLN-CLSSRSIHAAASAPHEGAATGLYGFEQLKSPKGFQ 60

Query: 61  RFVDEAIERSGELVTFISSKPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA 120
           RFVDEAIERSGELVTFISS PSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA
Sbjct: 61  RFVDEAIERSGELVTFISSMPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA 120

Query: 121 NKAAMRLNEYFHYLNTNHTLYNAVKKAEHEAHLLTREAHKAAHYLRVDFERAGIHLSAER 180
           NKAAMR++EYFH+LNTNHTLYNAVKKAEHEAHLLTREAH AAH+LRVDFERAGIHLSAER
Sbjct: 121 NKAAMRMSEYFHFLNTNHTLYNAVKKAEHEAHLLTREAHMAAHFLRVDFERAGIHLSAER 180

Query: 181 LDRVNQLSIEISQLCQEFKENIVIDPGSVDIFPPLCMPNKLHHLVKPIYRSSDSFGSRKG 240
           LDRVNQLSIEISQLCQE+KENIVIDPGSVDIFPP+ +PN LHHLVKPIYRSS SFGS   
Sbjct: 181 LDRVNQLSIEISQLCQEYKENIVIDPGSVDIFPPMRVPNNLHHLVKPIYRSSKSFGSISS 240

Query: 241 MKENGFRLVTDADTLYSVLQYASDDEVRKMAYIKGNSSPRANLDVLDKLIATRHELAQIV 300
           MKE GFRL+TDAD+L S+LQY SDDEVRKMAY+KGNSSPRANLDVLDK+IATRHELAQI+
Sbjct: 241 MKEKGFRLMTDADSLSSILQYTSDDEVRKMAYVKGNSSPRANLDVLDKIIATRHELAQIL 300

Query: 301 GYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCADEEFNQIREFKLKKCVNKSEDLEP 360
           GYRSYAEFAVTPN+ASSPAVV+SFLQELS +VRP ADEEFNQIREFKLKK  NK EDLEP
Sbjct: 301 GYRSYAEFAVTPNMASSPAVVVSFLQELSNLVRPSADEEFNQIREFKLKKRFNKFEDLEP 360

Query: 361 WDEAYYTSMMKSTAYNLDSSA-TSYFPLSQCIEGLKILVESLFGASFYSIPLAPGESWHP 420
           WDEAYYTSMMKSTAYNL+SS  +SYFPL+QCIEGLK LV SLFGASF SI LAPGESWHP
Sbjct: 361 WDEAYYTSMMKSTAYNLNSSVISSYFPLTQCIEGLKTLVNSLFGASFNSISLAPGESWHP 420

Query: 421 DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIRGGRKVSEMEYQLPVVALVCNFS 480
           DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAI+GG KVSEMEYQLPVVALVCNFS
Sbjct: 421 DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIQGGCKVSEMEYQLPVVALVCNFS 480

Query: 481 SSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS 540
            S  RSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS
Sbjct: 481 GSRERSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS 540

Query: 541 DYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEATELQRQILYALIDQTLFGERLTSER 600
           DYRVLRTFAKHYSTG++IPEKLVKSMQGAKKMF ATELQRQILYALIDQTLFGERLT ER
Sbjct: 541 DYRVLRTFAKHYSTGEVIPEKLVKSMQGAKKMFAATELQRQILYALIDQTLFGERLTLER 600

Query: 601 DTSSVVAELKRQYTNWKHVDDTHWQTRFCHLLTYGAGYYSYLYAKCFSATIWEKLCQEDP 660
           DTSSVVAELKRQ T+WKHVD THWQTRF HLLTYGAGYYSYLYAKCF+ATIWEKLCQEDP
Sbjct: 601 DTSSVVAELKRQCTSWKHVDGTHWQTRFFHLLTYGAGYYSYLYAKCFAATIWEKLCQEDP 660

Query: 661 LSRNTGTALRTKFLQHGGSKEAVDLLTDLVGDGIVRYCDGGVIPDLTSLCKEMDLSGSL 718
           LSR+TGTALRTKFLQHGGSKE VDLL DL+GDGI+RYCDGGVIPD+TSLCKEMD+SG L
Sbjct: 661 LSRSTGTALRTKFLQHGGSKEPVDLLKDLLGDGIIRYCDGGVIPDITSLCKEMDISGKL 718

BLAST of MC10g0202 vs. ExPASy TrEMBL
Match: A0A0A0L676 (Peptidase_M3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G176300 PE=3 SV=1)

HSP 1 Score: 1269 bits (3285), Expect = 0.0
Identity = 632/719 (87.90%), Postives = 677/719 (94.16%), Query Frame = 0

Query: 1   MSSLIRRTTPKLSSGAALKSCFSNRHRSRRSIHAPASAPQKGAATGLYGFEHLKSPKGFR 60
           MSSLIR+T  KL+SG  LKS + N  RSR SIHAPASAPQKGAATGLYGF+HLKSP+GFR
Sbjct: 1   MSSLIRKTPGKLTSGLVLKSSYWNCFRSR-SIHAPASAPQKGAATGLYGFDHLKSPQGFR 60

Query: 61  RFVDEAIERSGELVTFISSKPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA 120
           RFVDEAIERSGELVTFISS PSSAEVIRAMDEISN VCSVFDSAELCRQTHPDREFVEEA
Sbjct: 61  RFVDEAIERSGELVTFISSMPSSAEVIRAMDEISNSVCSVFDSAELCRQTHPDREFVEEA 120

Query: 121 NKAAMRLNEYFHYLNTNHTLYNAVKKAEHEAHLLTREAHKAAHYLRVDFERAGIHLSAER 180
           N AAMR+NEYFH+LNTNHTLY+AVKKAEHEAHLLTREAH AAHYLRVDFERAGIHLSA++
Sbjct: 121 NNAAMRMNEYFHFLNTNHTLYSAVKKAEHEAHLLTREAHMAAHYLRVDFERAGIHLSADK 180

Query: 181 LDRVNQLSIEISQLCQEFKENIVIDPGSVDIFPPLCMPNKLHHLVKPIYRSSDSFGSRKG 240
           LDRVNQL+IEISQLCQEFKENI+IDPG VDIFPPL MPN LHHL KPIYRSS+SFGSR  
Sbjct: 181 LDRVNQLNIEISQLCQEFKENILIDPGYVDIFPPLRMPNNLHHLAKPIYRSSESFGSRSS 240

Query: 241 MKENGFRLVTDADTLYSVLQYASDDEVRKMAYIKGNSSPRANLDVLDKLIATRHELAQIV 300
            KENGFRL+TD+D+L S+LQ+ASDDEVRKMAY+KGNSSPRANL VLDKLIATRH LAQI+
Sbjct: 241 KKENGFRLMTDSDSLSSILQFASDDEVRKMAYVKGNSSPRANLGVLDKLIATRHSLAQIL 300

Query: 301 GYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCADEEFNQIREFKLKKCVNKSEDLEP 360
           GYRS+AEFAVTPNLASSPAVVMSFLQELSKVVR  ADEEFNQIREFKLKKC+NK EDLEP
Sbjct: 301 GYRSFAEFAVTPNLASSPAVVMSFLQELSKVVRSRADEEFNQIREFKLKKCINKFEDLEP 360

Query: 361 WDEAYYTSMMKSTAYNLDSSA-TSYFPLSQCIEGLKILVESLFGASFYSIPLAPGESWHP 420
           WDEAYYTSMMKSTAYNLDSS   SYFPLSQCIEGLK LV+SLFGASFY+IPLAPGESWHP
Sbjct: 361 WDEAYYTSMMKSTAYNLDSSVIASYFPLSQCIEGLKTLVKSLFGASFYNIPLAPGESWHP 420

Query: 421 DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIRGGRKVSEMEYQLPVVALVCNFS 480
           DVLKLSL HPEEG+LG+LYLDLYSRKGKYPGCAHFAIRGGRKVSE EYQLPVVALVCNFS
Sbjct: 421 DVLKLSLQHPEEGELGFLYLDLYSRKGKYPGCAHFAIRGGRKVSETEYQLPVVALVCNFS 480

Query: 481 SSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS 540
           SS+ RSNVRLNHSE+ETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS
Sbjct: 481 SSNDRSNVRLNHSELETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS 540

Query: 541 DYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEATELQRQILYALIDQTLFGERLTSER 600
           DYRVL+TFAKHYSTG+I+PEKLVKSM+GAK MF ATELQRQILYALIDQTLFGE+LTSER
Sbjct: 541 DYRVLKTFAKHYSTGEILPEKLVKSMKGAKMMFAATELQRQILYALIDQTLFGEKLTSER 600

Query: 601 DTSSVVAELKRQYTNWKHVDDTHWQTRFCHLLTYGAGYYSYLYAKCFSATIWEKLCQEDP 660
           DT SVVA+LKRQYT+WKHVD THWQ++FCHLLTYGAGYY+YLYAKCF+ATIWEKLC+EDP
Sbjct: 601 DTVSVVADLKRQYTSWKHVDGTHWQSQFCHLLTYGAGYYTYLYAKCFAATIWEKLCKEDP 660

Query: 661 LSRNTGTALRTKFLQHGGSKEAVDLLTDLVGDGIVRYCDGGVIPDLTSLCKEMDLSGSL 718
           LSR TG ALRTKFLQHGGSKEAVDLLTDLVGDGI+RY +GGVIPD+TSLCKEM L+ +L
Sbjct: 661 LSRETGNALRTKFLQHGGSKEAVDLLTDLVGDGIIRYSEGGVIPDITSLCKEMGLTKNL 718

BLAST of MC10g0202 vs. ExPASy TrEMBL
Match: A0A5A7VHX4 (Putative mitochondrial intermediate peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold70G00530 PE=3 SV=1)

HSP 1 Score: 1254 bits (3245), Expect = 0.0
Identity = 625/719 (86.93%), Postives = 673/719 (93.60%), Query Frame = 0

Query: 1   MSSLIRRTTPKLSSGAALKSCFSNRHRSRRSIHAPASAPQKGAATGLYGFEHLKSPKGFR 60
           MSSL+R+TT KL+SG  LKS + N  R R SIHAPASAPQKGAATGLYGF+HLKSP+GFR
Sbjct: 1   MSSLVRKTTGKLTSGLVLKSSYWNCSRFR-SIHAPASAPQKGAATGLYGFDHLKSPQGFR 60

Query: 61  RFVDEAIERSGELVTFISSKPSSAEVIRAMDEISNRVCSVFDSAELCRQTHPDREFVEEA 120
           RFVDEAIERSGELVTFISS PSSAEVI+AMDEISN VCSVFDSAELCRQTHPDREFVEEA
Sbjct: 61  RFVDEAIERSGELVTFISSMPSSAEVIQAMDEISNSVCSVFDSAELCRQTHPDREFVEEA 120

Query: 121 NKAAMRLNEYFHYLNTNHTLYNAVKKAEHEAHLLTREAHKAAHYLRVDFERAGIHLSAER 180
           N AAMR+NEYFH+LNTNHTLY+AVKKAEHE HLLTREAH AAHYLRVDFERAGIHLSA++
Sbjct: 121 NNAAMRMNEYFHFLNTNHTLYSAVKKAEHETHLLTREAHMAAHYLRVDFERAGIHLSADK 180

Query: 181 LDRVNQLSIEISQLCQEFKENIVIDPGSVDIFPPLCMPNKLHHLVKPIYRSSDSFGSRKG 240
           LDRVNQL+IEISQLCQEFKENIVIDPG VDIFPPL MPN L+HL KPIYRS+   GSR  
Sbjct: 181 LDRVNQLNIEISQLCQEFKENIVIDPGYVDIFPPLRMPNNLYHLAKPIYRSNKLLGSRSS 240

Query: 241 MKENGFRLVTDADTLYSVLQYASDDEVRKMAYIKGNSSPRANLDVLDKLIATRHELAQIV 300
            +ENGFRL+TD+D+L S+LQ+ASDDEVRKMAY+KGNSSPRANL VLDKLIATRH LAQI+
Sbjct: 241 KEENGFRLMTDSDSLSSILQFASDDEVRKMAYVKGNSSPRANLGVLDKLIATRHSLAQIL 300

Query: 301 GYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCADEEFNQIREFKLKKCVNKSEDLEP 360
           GYRS+AEFAVTPNLASSPAVVMSFLQELSKVVR  ADEEFNQIREFKLKKC+NK EDLEP
Sbjct: 301 GYRSFAEFAVTPNLASSPAVVMSFLQELSKVVRSRADEEFNQIREFKLKKCMNKFEDLEP 360

Query: 361 WDEAYYTSMMKSTAYNLDSSA-TSYFPLSQCIEGLKILVESLFGASFYSIPLAPGESWHP 420
           WDEAYYTSMMKSTAYNLDSSA  SYFPLSQCIEGLK LV+SLFGASFY+IPLAPGESWHP
Sbjct: 361 WDEAYYTSMMKSTAYNLDSSAIASYFPLSQCIEGLKTLVKSLFGASFYNIPLAPGESWHP 420

Query: 421 DVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFAIRGGRKVSEMEYQLPVVALVCNFS 480
           DVLKLSL HPEEG+LG+LYLDLYSRKGKYPGCAHFAI+GGRKVSE EYQLPVVALVCNFS
Sbjct: 421 DVLKLSLQHPEEGELGFLYLDLYSRKGKYPGCAHFAIKGGRKVSETEYQLPVVALVCNFS 480

Query: 481 SSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS 540
           SS+ RSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS
Sbjct: 481 SSNDRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTRVVLDLAETPSNLFEYYAS 540

Query: 541 DYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEATELQRQILYALIDQTLFGERLTSER 600
           DYRVL+TFAKHYSTG+IIPEKLVKSMQGAK MF ATELQRQILYALIDQTLFGE+LTSER
Sbjct: 541 DYRVLKTFAKHYSTGEIIPEKLVKSMQGAKMMFAATELQRQILYALIDQTLFGEKLTSER 600

Query: 601 DTSSVVAELKRQYTNWKHVDDTHWQTRFCHLLTYGAGYYSYLYAKCFSATIWEKLCQEDP 660
            T+SVVA+LKRQYT+WKHVD THWQ++FCHLLTYGAGYY+YLYAKCF+ATIWEKLC+EDP
Sbjct: 601 GTASVVADLKRQYTSWKHVDGTHWQSQFCHLLTYGAGYYTYLYAKCFAATIWEKLCKEDP 660

Query: 661 LSRNTGTALRTKFLQHGGSKEAVDLLTDLVGDGIVRYCDGGVIPDLTSLCKEMDLSGSL 718
           LSR TG ALRTKFL+HGGSKE VDLLTDLVGDGI+RY +GGVIPD+TSLCKEM L+ +L
Sbjct: 661 LSRETGNALRTKFLEHGGSKEPVDLLTDLVGDGIIRYREGGVIPDITSLCKEMGLTKNL 718

BLAST of MC10g0202 vs. TAIR 10
Match: AT5G51540.1 (Zincin-like metalloproteases family protein )

HSP 1 Score: 946.4 bits (2445), Expect = 1.3e-275
Identity = 463/679 (68.19%), Postives = 551/679 (81.15%), Query Frame = 0

Query: 42  GAATGLYGFEHLKSPKGFRRFVDEAIERSGELVTFISSKPSSAEVIRAMDEISNRVCSVF 101
           G ATGLYGF+HLK+ KGF+RFV +AIERSGELV++IS  PSS E+I+AMDEIS+ VC V 
Sbjct: 28  GDATGLYGFDHLKTAKGFQRFVADAIERSGELVSYISGMPSSPEIIKAMDEISDTVCCVV 87

Query: 102 DSAELCRQTHPDREFVEEANKAAMRLNEYFHYLNTNHTLYNAVKKAEHEAHLLTREAHKA 161
           DSAELCRQTHPDREFVEEANKAA+ +N+Y H+LNTNHTLY AVKKAE +++LLT+EA + 
Sbjct: 88  DSAELCRQTHPDREFVEEANKAAIEMNDYLHHLNTNHTLYAAVKKAEQDSNLLTKEASRT 147

Query: 162 AHYLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEFKENIVIDPGSVDIFPPLCMPNKL 221
           AH+LR+DFER GIHL  E+LD+VN L+  I QLC+EF ENI  DPG VDIFP   +P  L
Sbjct: 148 AHHLRMDFERGGIHLDPEKLDKVNNLTTNIFQLCREFSENIADDPGHVDIFPGSRIPRHL 207

Query: 222 HHLVKPIYRSSD--SFGSRKG---MKENGFRLVTDADTLYSVLQYASDDEVRKMAYIKGN 281
           HHL+ P YRS+   S GS +     K+ GFR+ TD  T+ S+LQ+ SD+EVRKM YI+GN
Sbjct: 208 HHLLNPTYRSTSGGSRGSTRSAHKSKQKGFRINTDPRTVSSILQWTSDEEVRKMVYIQGN 267

Query: 282 SSPRANLDVLDKLIATRHELAQIVGYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCA 341
           S P AN  VL+KLIA RHEL+Q++G  SYA+  V PNLA SP VV SFLQELSK V+P A
Sbjct: 268 SVPHANHGVLEKLIAARHELSQMMGCNSYADIMVEPNLAKSPKVVTSFLQELSKTVKPKA 327

Query: 342 DEEFNQIREFKLKKCVNKSEDLEPWDEAYYTSMMKSTAYNLDSS-ATSYFPLSQCIEGLK 401
           DEEF  IR+FK +KC N S +LEPWDE YYTSMMKS+  ++D++   SYFPL QCIEGLK
Sbjct: 328 DEEFIAIRDFKREKCGNPSAELEPWDETYYTSMMKSSINDVDTAVVASYFPLPQCIEGLK 387

Query: 402 ILVESLFGASFYSIPLAPGESWHPDVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHFA 461
           +LVESLFGA+F++IPLAPGESWHP+V+KLSLHHP+EGDLGYLYLDLYSRKGKYPGCA FA
Sbjct: 388 VLVESLFGATFHTIPLAPGESWHPNVVKLSLHHPDEGDLGYLYLDLYSRKGKYPGCASFA 447

Query: 462 IRGGRKVSEMEYQLPVVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQ 521
           IRGGRK+SE EYQLPV+ALVCNFS +   S V+LNHSEVE LFHEFGHALHSLLSRT+YQ
Sbjct: 448 IRGGRKISETEYQLPVIALVCNFSRACDSSIVKLNHSEVEVLFHEFGHALHSLLSRTDYQ 507

Query: 522 HFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEAT 581
           HFSGTRV LDLAE PSNLFEYYA DYR+L+ FA+HYSTG+ IPEKLV S+QGA+ MF AT
Sbjct: 508 HFSGTRVALDLAEMPSNLFEYYAWDYRLLKRFARHYSTGETIPEKLVNSLQGARNMFAAT 567

Query: 582 ELQRQILYALIDQTLFGERLTSERDTSSVVAELKRQYTNWKHVDDTHWQTRFCHLLTYGA 641
           E+QRQ+ YALIDQ LFGE+  + RD S +VAELKRQ+T+W HV+ THW  RF HLL YGA
Sbjct: 568 EMQRQVFYALIDQMLFGEQPETARDVSHLVAELKRQHTSWNHVEGTHWYIRFSHLLNYGA 627

Query: 642 GYYSYLYAKCFSATIWEKLCQEDPLSRNTGTALRTKFLQHGGSKEAVDLLTDLVGDGIVR 701
           GYYSYLYAKCF++TIW+ +C+EDPLS NTGT LR KF +HGG+K+  +LLTDL G  I+ 
Sbjct: 628 GYYSYLYAKCFASTIWQSICEEDPLSLNTGTLLREKFFKHGGAKDPAELLTDLAGKEIIS 687

Query: 702 YCDGGVIPDLTSLCKEMDL 715
               G++P  T L  E+ L
Sbjct: 688 VHGEGIVPATTYLLNELRL 706

BLAST of MC10g0202 vs. TAIR 10
Match: AT1G67690.1 (Zincin-like metalloproteases family protein )

HSP 1 Score: 180.3 bits (456), Expect = 5.7e-45
Identity = 160/669 (23.92%), Postives = 303/669 (45.29%), Query Frame = 0

Query: 37  SAPQKGAATGLYGFEHL---KSPKGFRRFVDEAIERS---GELVTFIS-SKPSSAEVIRA 96
           S  QK    GL G + +    S K      +E I +S    + V  +S  K S   V+  
Sbjct: 45  SHKQKSKKKGLPGCDTVCVNLSAKEILDLAEEIIHKSTRVHDAVALVSLDKLSYENVVLP 104

Query: 97  MDEISNRVCSVFDSAELCRQTHPDREFVEEANKAAMRLNEYFHYLNTNHTLYNAVKKAEH 156
           + E+  R  S+       +   P     + + +A  +++ +         +Y  +K    
Sbjct: 105 LAELEARQLSLIQCCVFPKMLSPHDNVRKASTEAEQKIDAHILSCRKREDVYRIIKIYAA 164

Query: 157 EAHLLTREAHKAAHYLRVDFERAGIHLSAERLDRVNQLSIEISQLCQEFKENIVIDPGSV 216
           +   ++ EA      L  DFE  G++L+A + + V +L  EI +L   + +N+  D   +
Sbjct: 165 KGESISPEAKCYLQCLVRDFEDNGLNLTAIKREEVERLKYEIDELSLRYIQNLNEDSSCL 224

Query: 217 DIFPPLCMPNKLHHLVKPIYRSSDSFGSRKGMKENGFRLVTDADTLYSVLQYASDDEVRK 276
                    ++L  L     ++ +   +++      F+L  ++  + ++L+     + RK
Sbjct: 225 -----FFTEDELAGLPLEFLQNLEKTQNKE------FKLTLESRHVAAILELCKIAKTRK 284

Query: 277 -MAYIKGNSSPRANLDVLDKLIATRHELAQIVGYRSYAEFAVTPNLASSPAVVMSFLQEL 336
            +A   G      N+ VL +L+ +RH LA + GY  +A++A+   ++ +   V+ FL+++
Sbjct: 285 TVAMAYGKRCGDTNIPVLQRLVQSRHRLACVCGYAHFADYALDRRMSKTSMRVIRFLEDI 344

Query: 337 SKVVRPCADEEFNQIREFKLKKCVNKSEDLEPWDEAYYTSMMKSTAYNLD-SSATSYFPL 396
           S  +   A  EF+ + + K K+       +E  D  YY   ++   ++LD      YFP+
Sbjct: 345 SSSLTDLAIREFSILEDLKRKEEGEIPFGVE--DLLYYIKRVEELQFDLDFGDIRQYFPV 404

Query: 397 SQCIEGLKILVESLFGASFYSIPLAPGESWHPDVLKLSLHHPEEGD-LGYLYLDLYSRKG 456
           +  + G+  + + LFG  F  +     + W+ D+   ++     G  LGY YLD+++R+G
Sbjct: 405 NLVLSGIFKICQDLFGIKFEEV--TEVDVWYHDIRAFAVFDSGSGKLLGYFYLDMFTREG 464

Query: 457 KYPGCAHFAIRGGRKVSEMEYQLPVVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALH 516
           K       A++     S    Q+PV  L+  F+       V L  S+V  LFHEFGH + 
Sbjct: 465 KCNHSCVVALQNNALFSNGACQIPVALLIAQFAKDGSGEAVPLGFSDVVNLFHEFGHVVQ 524

Query: 517 SLLSRTEYQHFSGTRVVLDLAETPSNLFEYYASDYRVLRTFAKHYSTGDI---IPEKLVK 576
            + +R  +  FSG RV  D  E PS L E +  +   L+  + +    DI   + +++ K
Sbjct: 525 HICNRASFARFSGLRVDPDFREIPSQLLENWCYESFTLKLISGYRQ--DITKPLVDEVCK 584

Query: 577 SMQGAKKMFEATELQRQILYALIDQTLFGERLTSERDTSSVVAEL-KRQYTNWKHVDDTH 636
           +++  +  F A +  ++ILY L DQ ++ +    + D   ++  L  +       V+ T+
Sbjct: 585 TLKRWRYSFSALKSLQEILYCLFDQIIYSD---DDADLLQLIRSLHPKVMIGLPVVEGTN 644

Query: 637 WQTRFCH-LLTYGAGYYSYLYAKCFSATIWEKLCQEDPLSRNTGTALRTKFLQHGGSKEA 691
             + F   ++   A  YS L+++ ++A I+     +   +   G   R K L  GG KE 
Sbjct: 645 PASCFPRAVIGSEATCYSRLWSEVYAADIFASKFGDGHPNLYAGLQFRDKVLAPGGGKEP 693

BLAST of MC10g0202 vs. TAIR 10
Match: AT5G65620.1 (Zincin-like metalloproteases family protein )

HSP 1 Score: 166.4 bits (420), Expect = 8.5e-41
Identity = 151/648 (23.30%), Postives = 286/648 (44.14%), Query Frame = 0

Query: 78  SSKPSSAEVIRAMDEISNR---VCSVFDSAELCRQTHPDREFVEEANKAAMRLNEYFHYL 137
           S +P+  +++  +++I +R   V  + +  +  + T   R  +E+     ++   +   L
Sbjct: 138 SVEPTWPKLVEPLEKIVDRLTVVWGMINHLKAVKDTPELRAAIEDVQPEKVK---FQLRL 197

Query: 138 NTNHTLYNAVKKAEHEAHLLTREAHKAAHYLRVDFERA---GIHLSAERLDRVNQLSIEI 197
             +  +YNA  KA  E+   +  +      +    + A   GI L  E+ +  N++  E+
Sbjct: 198 GQSKPIYNAF-KAIRESPDWSSLSEARQRLVEAQIKEAVLIGIALDDEKREEFNKIEQEL 257

Query: 198 SQLCQEFKENI----------VIDPGSVDIFPPLCMPNKLHHLVKPIYRSSDSFGSRKGM 257
            +L  +F EN+          + D   ++  PP  +            +++ S G     
Sbjct: 258 EKLSHKFSENVLDATKKFEKLITDKKEIEGLPPSAL--------GLFAQAAVSKGHENAT 317

Query: 258 KENGFRLVT-DADTLYSVLQYASDDEVRKMAY----IKGNSSPRANLDVLDKLIATRHEL 317
            ENG  ++T DA +   V+Q+A +  +R+  Y     + +S    N  ++D+++  R E 
Sbjct: 318 AENGPWIITLDAPSYLPVMQHAKNRALREEVYRAYLSRASSGDLDNTAIIDQILKLRLEK 377

Query: 318 AQIVGYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCADEEFNQIREFKLKKCVNKSE 377
           A+++GY +YAE ++   +A+        L++L       A ++   ++ F   +   +S+
Sbjct: 378 AKLLGYNNYAEVSMAMKMATVEKAA-ELLEKLRSASWDAAVQDMEDLKSFAKNQGAAESD 437

Query: 378 DLEPWDEAYYTSMMKSTAYNL-DSSATSYFPLSQCIEGLKILVESLFGASFYSIP-LAPG 437
            +  WD  +++  ++ + Y++ +     YF L + ++GL  L ++LFG        LAP 
Sbjct: 438 SMTHWDTTFWSERLRESKYDINEEELRPYFSLPKVMDGLFSLAKTLFGIDIEPADGLAP- 497

Query: 438 ESWHPDVLKLSLHHPEEGDLGYLYLDLYSRKGKYPGCAHF--AIRGGRKVSE--MEYQLP 497
             W+ DV    +       + Y Y D YSR  +  G A     +   R +++     +LP
Sbjct: 498 -VWNNDVRFYRVKDSSGNPIAYFYFDPYSRPSEKRGGAWMDEVVSRSRVMAQKGSSVRLP 557

Query: 498 VVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTR-VVLDLAET 557
           V  +VCN +   G     +   EVET+FHEFGHAL  +L++ +    +G R +  D  E 
Sbjct: 558 VAHMVCNQTPPVGDKPSLMTFREVETVFHEFGHALQHMLTKQDEGLVAGIRNIEWDAVEL 617

Query: 558 PSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEATELQRQILYALIDQT 617
           PS   E +      L + AKHY TG+ +PE++ K +  A+     +   RQ+ +A +D  
Sbjct: 618 PSQFMENWCYHRDTLMSIAKHYETGETLPEEVYKKLLAARTFRAGSFSLRQLKFASVDLE 677

Query: 618 LFGERLTSERDTSSVVAELKRQYTN-WKHVDDTHWQTRFCHLLT--YGAGYYSYLYAKCF 677
           L  + +    ++   V +     T     + +  +   F H+    Y AGYYSY +A+  
Sbjct: 678 LHTKYVPGGPESIYDVDQRVSVKTQVIPPLPEDRFLCSFSHIFAGGYAAGYYSYKWAEVL 737

Query: 678 SATIWEKL----CQEDPLSRNTGTALRTKFLQHGGSKEAVDLLTDLVG 691
           SA  +         +    + TG   R   L  GG K  + +  +  G
Sbjct: 738 SADAFSAFEDAGLDDIKAVKETGQRFRNTILALGGGKAPLKVFVEFRG 770

BLAST of MC10g0202 vs. TAIR 10
Match: AT5G10540.1 (Zincin-like metalloproteases family protein )

HSP 1 Score: 161.0 bits (406), Expect = 3.6e-39
Identity = 149/650 (22.92%), Postives = 282/650 (43.38%), Query Frame = 0

Query: 80  KPSSAEVIRAMDEISNR---VCSVFDSAELCRQTHPDREFVEEANKAAMRLNEYFHYLNT 139
           +PS  +++  +++I +R   V  + +  +  + T   R  +EE     ++   +   L  
Sbjct: 52  EPSWPKLVEPLEKIIDRLSVVWGMINHLKAVKDTPELRAAIEEVQPEKVK---FQLRLGQ 111

Query: 140 NHTLYNAVK--KAEHEAHLLTREAHKAAHYLRVDFERAGIHLSAERLDRVNQLSIEISQL 199
           +  +YNA K  +   + + L+    +       +   +GI L  ++ +  N++  E+ +L
Sbjct: 112 SKPIYNAFKAIRESPDWNSLSEARQRLVEAQIKEAVLSGIALEDDKREEFNKIEQELEKL 171

Query: 200 CQEFKENI----------VIDPGSVDIFPPLCMPNKLHHLVKPIYRSSDSFGSRKGMKEN 259
             +F EN+          + D   ++  PP  +            +++ S G      + 
Sbjct: 172 SHKFSENVLDATKKFEKLITDKKEIEGLPPSAL--------GLFAQAAVSKGHETATADT 231

Query: 260 GFRLVT-DADTLYSVLQYASDDEVRKMAY----IKGNSSPRANLDVLDKLIATRHELAQI 319
           G  L+T DA +   V+Q+A +  +R+  Y     + +S    N  ++D+++  R E A++
Sbjct: 232 GPWLITLDAPSYLPVMQHAKNRALREEVYRAYLSRASSGDLDNTAIIDQILKLRLEKAKL 291

Query: 320 VGYRSYAEFAVTPNLASSPAVVMSFLQELSKVVRPCADEEFNQIREFKLKKCVNKSEDLE 379
           +GYR+YAE ++   +A+        L++L       A ++   ++ F   +   +++ L 
Sbjct: 292 LGYRNYAEVSMATKMATVEK-ADELLEKLRSASWDPAVQDIEDLKSFAKNQGAAEADSLT 351

Query: 380 PWDEAYYTSMMKSTAYNL-DSSATSYFPLSQCIEGLKILVESLFGASFYSIPLAPGES-- 439
            WD  +++  ++ + Y++ +     YF L + ++ L  L ++LFG     I + P +   
Sbjct: 352 HWDITFWSERLRESKYDINEEELRPYFSLPKVMDALFGLAKTLFG-----IDVVPADGVA 411

Query: 440 --WHPDVLKLSLHHPEEGDLGYLYLDLYSRKG-KYPGCAHFAIRGGRKVSEME---YQLP 499
             W+ DV    +         Y Y D YSR   K  G     +    +V   +    +LP
Sbjct: 412 PVWNSDVRFYCVKDSSGNPTAYFYFDPYSRPSEKRDGAWMDEVFSRSRVMAQKGSSVRLP 471

Query: 500 VVALVCNFSSSHGRSNVRLNHSEVETLFHEFGHALHSLLSRTEYQHFSGTR-VVLDLAET 559
           V  +VCN +   G     +   EVET+FHEFGHAL  +L++ +    +G R +  D  E 
Sbjct: 472 VAQMVCNQTPPVGDKPSLMTFREVETVFHEFGHALQHMLTKEDEGLVAGIRNIEWDAVEL 531

Query: 560 PSNLFEYYASDYRVLRTFAKHYSTGDIIPEKLVKSMQGAKKMFEATELQRQILYALIDQT 619
           PS   E +      L + AKHY TG+ +PE + K +  A+     +   RQ+ +A +D  
Sbjct: 532 PSQFMENWCYHRDTLMSIAKHYQTGETLPENVYKKLLAARTFRAGSLSLRQLKFATVDLE 591

Query: 620 LFGERLTSERDTSSVV---AELKRQYTNWKHVDDTHWQTRFCHLLT--YGAGYYSYLYAK 679
           L  + +    +T   V     +K Q      + +  +   F H+    Y AGYYSY +A+
Sbjct: 592 LHTKYMPGGAETIYEVDQRVSIKTQVI--PPLPEDRFLCSFSHIFAGGYAAGYYSYKWAE 651

Query: 680 CFSATIWEKL----CQEDPLSRNTGTALRTKFLQHGGSKEAVDLLTDLVG 691
             SA  +         +    + TG   R   L  GG K  + +  +  G
Sbjct: 652 VLSADAFSAFEDAGLDDIKAVKETGQRFRNTILALGGGKAPLKVFVEFRG 682

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4KDA51.8e-27468.19Mitochondrial intermediate peptidase, mitochondrial OS=Arabidopsis thaliana OX=3... [more]
Q5RF145.6e-10634.12Mitochondrial intermediate peptidase OS=Pongo abelii OX=9601 GN=MIPEP PE=2 SV=1[more]
Q997971.3e-10534.12Mitochondrial intermediate peptidase OS=Homo sapiens OX=9606 GN=MIPEP PE=1 SV=2[more]
A6H6115.8e-10334.46Mitochondrial intermediate peptidase OS=Mus musculus OX=10090 GN=Mipep PE=1 SV=1[more]
Q019923.5e-10034.61Mitochondrial intermediate peptidase OS=Rattus norvegicus OX=10116 GN=Mipep PE=1... [more]
Match NameE-valueIdentityDescription
XP_022141120.10.099.56probable mitochondrial intermediate peptidase, mitochondrial, partial [Momordica... [more]
XP_022941716.10.089.29mitochondrial intermediate peptidase, mitochondrial isoform X1 [Cucurbita moscha... [more]
XP_022972575.10.089.01mitochondrial intermediate peptidase, mitochondrial isoform X1 [Cucurbita maxima... [more]
XP_023518581.10.089.01mitochondrial intermediate peptidase, mitochondrial isoform X1 [Cucurbita pepo s... [more]
XP_004148134.10.087.90mitochondrial intermediate peptidase, mitochondrial isoform X3 [Cucumis sativus]... [more]
Match NameE-valueIdentityDescription
A0A6J1CH320.099.56probable mitochondrial intermediate peptidase, mitochondrial OS=Momordica charan... [more]
A0A6J1FLV90.089.29mitochondrial intermediate peptidase, mitochondrial isoform X1 OS=Cucurbita mosc... [more]
A0A6J1I9110.089.01mitochondrial intermediate peptidase, mitochondrial isoform X1 OS=Cucurbita maxi... [more]
A0A0A0L6760.087.90Peptidase_M3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G17630... [more]
A0A5A7VHX40.086.93Putative mitochondrial intermediate peptidase OS=Cucumis melo var. makuwa OX=119... [more]
Match NameE-valueIdentityDescription
AT5G51540.11.3e-27568.19Zincin-like metalloproteases family protein [more]
AT1G67690.15.7e-4523.92Zincin-like metalloproteases family protein [more]
AT5G65620.18.5e-4123.30Zincin-like metalloproteases family protein [more]
AT5G10540.13.6e-3922.92Zincin-like metalloproteases family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001567Peptidase M3A/M3B catalytic domainPFAMPF01432Peptidase_M3coord: 258..691
e-value: 2.6E-119
score: 399.5
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 386..537
e-value: 1.6E-140
score: 471.0
IPR024077Neurolysin/Thimet oligopeptidase, domain 2GENE3D1.10.1370.10Neurolysin, domain 3coord: 182..690
e-value: 1.6E-140
score: 471.0
NoneNo IPR availablePANTHERPTHR11804:SF79MITOCHONDRIAL INTERMEDIATE PEPTIDASE, MITOCHONDRIALcoord: 30..714
NoneNo IPR availableCDDcd06457M3A_MIPcoord: 46..691
e-value: 0.0
score: 764.021
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 55..690
IPR045090Peptidase M3A/M3BPANTHERPTHR11804PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATEDcoord: 30..714

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0202.1MC10g0202.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006518 peptide metabolic process
biological_process GO:0006508 proteolysis
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008237 metallopeptidase activity