MC10g0127 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC10g0127
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Cytochrome P450
Location	MC10: 912154 .. 917150 (-)
RNA-Seq Expression	MC10g0127
Synteny	MC10g0127

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGGTTTTGTTGGTGAGAGTTGTATTTTCATGGTGGATTTTGCCGGTTTTGGCTCACAGAAAGCTCAAGCGAAACGGCTTTAATGGAGGCCCTCCTCCAAGATTCCCTCTCGGAAATATTACTGAGATCAAGAACACGATGAAGAAAGCTGCTGCTTCTGCAGCCGCCGCCGCCATTAATGGCGGTGCAGGCGCTTTTAGTTCATCATCAAATATCTCCCACGATATTCATTCCACTGTTTTCCCTTACTTCGCTCAATGGCAGACCTCTTATGGTAATCAAACTATACCCTCTTTATCTCTTATATATTTTCAAATACCATTTTTAACAAAATATATATATAGAAAATCTTTGCAGTGTTGTTTCCTTCTTATTTGTTATCATACTTGTTGGGGTTCGTGTAATTATCCAAATCTACAAGTGAGGAGAGTGTTAGATATATATCTATAAATTTGTTCGATCCATCCACTTAAATTTTTGAATTGAATGGTGGTTTATTCATCCTTAATAAACTTCAACTGAAAAAATGTAATTAAATTACAATAAACTAAAAGAGTTAGGATAAATTTAATTCGTTTACACATGTTTCGATTTGTTAAATGTTAAGTGCTTTCTTTTTATATATTTTTTTTCTGTATCTAATAATATTTTAAGACAAAGCTGCAGGTGATGATAATTGAATATTATTTTGAGAAAGATTAGGCATATCTAATTATTCTCATTACAATAATATTTCAATGGCTATTTGTCCATCGTCTAAGCATTTTCTTAAATCTTATAAACAAAAATATAATGAATTCTATAAATGGGACGTAAGCGGAGTTAGCTACTATTTTATTAGGTTAGAGATTCAAATTTTCATACTCATGTGCCGTATAATAAATTTAATGAGATAGAAAATTAATAATAACAAAACAAGCTTACTATTTTATCAATTTTCTTTTGTTTTTTTATTGAAAAGAAATAACTGCTATGTTAACAAACAAATGACATACCGCACATAGAAGAACAACTTTTAGGATCCAATAAATTAAAATCAGTATATCTATTCATAACATATCTAAAAATATAATTTTCATATTTATTTCTCAATATTTAAAACTATTTAGCGCCTCTAAGGAACCTATTAAACCATGGATGAGAAATGGATTTTAGCTTTGGTCTAAGGAGACTAAGATTAATTAAGAAGGAATGGGTTCAAACCCCGTGGTGTTCACCCCTTCAAGAATTAATAATTACTATATGTTTTTCGAGACCAAATGTGTCCTAATAGGGATAGAGTCAGGAGAATAAGATGTGAACTGAGAAGAAAGGAAAAGAATAAGTGTTGGAAGTATATTTCTCCACCATGACTGAAAATAGATGACAAGATTTCTCTTTCCATAAACATAAAGTCTTATTCATGACTAACACAGTTGACAAATATGTCTCCTCCGACAAAATATATAATGGTCACATCACTGTGCATATAAATATCTTCAGTTAAGGTCTCCATATGTTTGTTGTAACAAAAACTAATGTCAAATTCAACCACAAAAATCAAGATCTAAGTTATACGGAAACTTTTAAAGTTGAAGAACTAGGAGAAAATATGATAGATACATAAAATGGAGGTTTTATATATGTTTTGTGTTGCAGGGAAGAAGTTTGTGTATTGGTTGGGGACAGAGCCATTTTTGTACATAGCAGAGCCGCAGTTTGTGAAGAACATGTCTGAAGCAGTGATGGGAAAGGGCTGGGGGAAGCCGGCGGTCTTCAGAAACGACAGAGAGCCGATGTTCGGCGACGGCCTCGTCATGACTGAAGGCGACGACTGGATTCGCCATCGCCATATACTCACCCCCGCCTTCAACCCAGCTAACTTGAAGGTAATTCTCACTTAACTTTCATTTGTCGAACATGTTCGATTCAAGCCGAGCTTATATTTAGTACATGAGTTCACTTAACTTTACATACTAATTTTGTTGTTGTAGTTCACTGGCTAAGTATATGTTATACAAATTTGACTACCAATTTTTGTGTACACTAAAATCTTAAGTTAAACAACTCAAATTTGTTAAAATTGTGAGTCAAAATGGTTGTGAATTAATATTATTTATGAGGGTATGAAAATCTAACATGACATTGCAAAATGAGGAGGGTTTTTTTCTTATTATTATTATTATCATCGTGATTGATTATTATATTTTTGGCAAAGGAAAAATTTAATATGTTTATATATGTGAAATGAAAACTGAAAAGCAACCGTTGGTGGTGGAGATATGACATATCACTCTATATGTAATGACAAACAAATTATATTAGTAGTTTATGGTTTGGTTGATAAGATTGGTTTTATCTCTTTTCTTTTAGAATATCCTCTTAATTTTTAGAGGGAAAAAAAAAAAGAGAGAGAGAAAAACCCTAACCTTTTTGTGTACAGAAAGCATGGCCAAATCAATTTTTATTAATAATTTTTATTTTTCTTAATTCATATTGAAAGTCAAATACATGTTAAAAAAAAGCTATCATCAAAATTAATCATAAAGTAATATTAAAAAGAACCAGATTTAACTACCGGCCATCAAAATGCTTAATGAAGAATTTAATTATATATTGAGATTTAAAACGTGCTTCTTTATTGAATAAAAAATATATATTAAACTTGATTTAAGAGAGTCTTACCTCATTTACTTTCTCACGTAATTGTTTATCTATATAATTAAGTAAATAATGCTTTTACTTTATAAATTTTTTAATTAAGTCATTTCGTTAGTGAAAGTAATTTCGCTTTTTTATAATTAATCACAAAATAAAGGCCACTAAAATTTTAAAAACACAATCAATCAGAATTAACCACGCTTAATAATTAATCAATTAATTAATTTAGTTTTTGTTTGTGTTTTTTTTTTTTAATTTTCAGGCAATGACAAGGCTGATGGTGGGGTCCACCACTAAAATGCTGGACGAGTGGGCCCGCCGCTTGAGCTCCGGCGGCCCAGAGATGGACGTGGAGAGAGAAATAACGGCGACCGCCGGCGACATAATCGCGAAGGCGAGCTTCGGAATATGCGATCGGAGCGGCAGAGACGCGTTCAACAAGCTCAGAGATCTCCAATTCACTCTCTTCCGGAACAGCAGCTACGTCGGCGTCCCGTTCAGCAGCATTTTCCTGTACCCGACCCGAACCCTGACCGCGAAGAGACTCGGATCCGAAATCGACCGCCTCTTCTTGTCGATCATATCGGAGAGGAAGCAATCGCCGGACGCCTCCGCCTCCGATTTGCTCAGTAGGTTGATTGAGAGTCAGAGGAACGATAGCGCCCCCGCCGCCGGAAAGTCGTGGCTGACGACGAGAGAACTGGTGGACGAGTGCAAGACGTTCTTCTTCGGCGGCCACGAGACGACGGCGCTGGCGATTAGTTGGACGATGCTGCTGCTGGCGACGAGGCCTGAGTGGCAAACCATTTTACGAGAAGAGATCAAAGAAGTTATCGGAGATAGAGAGATCGATTTCGCCATGCTTTCTCGACTCAAAAAGGTTAGGGTTTCTCTTTATCTTTATTAATTGGATTATGAATTTGGTTAGATTATTGCAATTTTCATTAGTTAATATATATATATATATAATAATGAAAAAGGTTGAGAATTAGGGTCAGAAATCCATGCGATGGAGTTTCACGCGAGAGTTATATGGATCGATTATTGGGTGTGGTTGTACGGCATGATATGACTTTTTGGCCCATAAGATCATAAAATATTCTCCTTTTTTTTCAAAATCATTTTTAATTCTGTTGAAGTAATGATTCGGTCTTATATTTTAAATCGGTAAATATTTAGTTATTAAGCTCAACTCAATACATACTAATTCAATACATACTAATTTAATCTATGTAGTTTAAAGTTTGTAAAAATTTAGTTGTCGTAAAAAGTAAATTTATGAGATTAAATATGTTTATAAACTATAAAAACTAAAATTTTGATACTTAATTGTAATGAGATTTTTCATGATTCAGATTCAATTGTTACAAGTTTTAAATTATAGAGATTAAATTATTATCTATATAGATTAAATTGTTACATACTAAAATTTTTAACATTCTTTCTAGTTTCGGAAGAGATTGCATTGTGCTCCTACTCTTTTGGAAATTACATTTGGAAAACTTGCACAAATTTATAATTTTTTTTTTCTTTTAGCGTACCCAACAACACGTGTGAGTTCTTTAATTTTCAAATGGATTCAATGTTTGACCAATAAAAATTCATCTAATCTTCGTATCTTTCTAACTTAAACTTAAGCTGATTTGAACATAGTTTAAATTAAGACCACATACATATATATCTTCGAATCTCTATCTTCCTGGACATGTTGAACTCAGAAAAATATATGTGATTACACTTTTACATTGTGTTAATAACTATTTTAAACTTTGTTTAGTATTTTCCCTTCTCCCATTTGCATATGGATCGGCACAATCCCATGCATTAATCTCAAACCCTAAAATCCCAATTCTAGAACAATCAAATTCATTTATAAAGAAATTACCTAATGATCATGACAAGGTGTGTGTTGATAAAATGTGCAGATGGGATGGGTGTTCAATGAGGTACTTAGGCTATACCCATCAGCCCCAAACATACAAAGACAAGCCAAAGGAGACATTAAAGTAGGCGACACGACGATCCCGAAAGGAACCAACATATGGATCGACATCGTAGCGATGCATCACGACCCGAGCCTTTGGGGCGATGACGTGAACGAGTTCAAGCCCGAGAGGTTCGAGAAGGACAGCATTCATGGCGGGTGCAAGCACAAGATGGGATATTTGCCATTTGGGTTTGGAGGGAGAATGTGCATTGGTAGAAACTTGTCTTCCATGGAGTATAAGATTGTGCTAACCCTAATTATTTCTAGGTTTTCTCTCTCTATTTCTCCCTCTTATTCTCATTCTCCTGCTATTTTGCTCTCTTTAAGACCTGCCCATGGCCTCCCTCTCATTCTAAAACCCCTTCACCCT

mRNA sequence

GTGGTTTTGTTGGTGAGAGTTGTATTTTCATGGTGGATTTTGCCGGTTTTGGCTCACAGAAAGCTCAAGCGAAACGGCTTTAATGGAGGCCCTCCTCCAAGATTCCCTCTCGGAAATATTACTGAGATCAAGAACACGATGAAGAAAGCTGCTGCTTCTGCAGCCGCCGCCGCCATTAATGGCGGTGCAGGCGCTTTTAGTTCATCATCAAATATCTCCCACGATATTCATTCCACTGTTTTCCCTTACTTCGCTCAATGGCAGACCTCTTATGGGAAGAAGTTTGTGTATTGGTTGGGGACAGAGCCATTTTTGTACATAGCAGAGCCGCAGTTTGTGAAGAACATGTCTGAAGCAGTGATGGGAAAGGGCTGGGGGAAGCCGGCGGTCTTCAGAAACGACAGAGAGCCGATGTTCGGCGACGGCCTCGTCATGACTGAAGGCGACGACTGGATTCGCCATCGCCATATACTCACCCCCGCCTTCAACCCAGCTAACTTGAAGGCAATGACAAGGCTGATGGTGGGGTCCACCACTAAAATGCTGGACGAGTGGGCCCGCCGCTTGAGCTCCGGCGGCCCAGAGATGGACGTGGAGAGAGAAATAACGGCGACCGCCGGCGACATAATCGCGAAGGCGAGCTTCGGAATATGCGATCGGAGCGGCAGAGACGCGTTCAACAAGCTCAGAGATCTCCAATTCACTCTCTTCCGGAACAGCAGCTACGTCGGCGTCCCGTTCAGCAGCATTTTCCTGTACCCGACCCGAACCCTGACCGCGAAGAGACTCGGATCCGAAATCGACCGCCTCTTCTTGTCGATCATATCGGAGAGGAAGCAATCGCCGGACGCCTCCGCCTCCGATTTGCTCAGTAGGTTGATTGAGAGTCAGAGGAACGATAGCGCCCCCGCCGCCGGAAAGTCGTGGCTGACGACGAGAGAACTGGTGGACGAGTGCAAGACGTTCTTCTTCGGCGGCCACGAGACGACGGCGCTGGCGATTAGTTGGACGATGCTGCTGCTGGCGACGAGGCCTGAGTGGCAAACCATTTTACGAGAAGAGATCAAAGAAGTTATCGGAGATAGAGAGATCGATTTCGCCATGCTTTCTCGACTCAAAAAGATGGGATGGGTGTTCAATGAGGTACTTAGGCTATACCCATCAGCCCCAAACATACAAAGACAAGCCAAAGGAGACATTAAAGTAGGCGACACGACGATCCCGAAAGGAACCAACATATGGATCGACATCGTAGCGATGCATCACGACCCGAGCCTTTGGGGCGATGACGTGAACGAGTTCAAGCCCGAGAGGTTCGAGAAGGACAGCATTCATGGCGGGTGCAAGCACAAGATGGGATATTTGCCATTTGGGTTTGGAGGGAGAATGTGCATTGGTAGAAACTTGTCTTCCATGGAGTATAAGATTGTGCTAACCCTAATTATTTCTAGGTTTTCTCTCTCTATTTCTCCCTCTTATTCTCATTCTCCTGCTATTTTGCTCTCTTTAAGACCTGCCCATGGCCTCCCTCTCATTCTAAAACCCCTTCACCCT

Coding sequence (CDS)

GTGGTTTTGTTGGTGAGAGTTGTATTTTCATGGTGGATTTTGCCGGTTTTGGCTCACAGAAAGCTCAAGCGAAACGGCTTTAATGGAGGCCCTCCTCCAAGATTCCCTCTCGGAAATATTACTGAGATCAAGAACACGATGAAGAAAGCTGCTGCTTCTGCAGCCGCCGCCGCCATTAATGGCGGTGCAGGCGCTTTTAGTTCATCATCAAATATCTCCCACGATATTCATTCCACTGTTTTCCCTTACTTCGCTCAATGGCAGACCTCTTATGGGAAGAAGTTTGTGTATTGGTTGGGGACAGAGCCATTTTTGTACATAGCAGAGCCGCAGTTTGTGAAGAACATGTCTGAAGCAGTGATGGGAAAGGGCTGGGGGAAGCCGGCGGTCTTCAGAAACGACAGAGAGCCGATGTTCGGCGACGGCCTCGTCATGACTGAAGGCGACGACTGGATTCGCCATCGCCATATACTCACCCCCGCCTTCAACCCAGCTAACTTGAAGGCAATGACAAGGCTGATGGTGGGGTCCACCACTAAAATGCTGGACGAGTGGGCCCGCCGCTTGAGCTCCGGCGGCCCAGAGATGGACGTGGAGAGAGAAATAACGGCGACCGCCGGCGACATAATCGCGAAGGCGAGCTTCGGAATATGCGATCGGAGCGGCAGAGACGCGTTCAACAAGCTCAGAGATCTCCAATTCACTCTCTTCCGGAACAGCAGCTACGTCGGCGTCCCGTTCAGCAGCATTTTCCTGTACCCGACCCGAACCCTGACCGCGAAGAGACTCGGATCCGAAATCGACCGCCTCTTCTTGTCGATCATATCGGAGAGGAAGCAATCGCCGGACGCCTCCGCCTCCGATTTGCTCAGTAGGTTGATTGAGAGTCAGAGGAACGATAGCGCCCCCGCCGCCGGAAAGTCGTGGCTGACGACGAGAGAACTGGTGGACGAGTGCAAGACGTTCTTCTTCGGCGGCCACGAGACGACGGCGCTGGCGATTAGTTGGACGATGCTGCTGCTGGCGACGAGGCCTGAGTGGCAAACCATTTTACGAGAAGAGATCAAAGAAGTTATCGGAGATAGAGAGATCGATTTCGCCATGCTTTCTCGACTCAAAAAGATGGGATGGGTGTTCAATGAGGTACTTAGGCTATACCCATCAGCCCCAAACATACAAAGACAAGCCAAAGGAGACATTAAAGTAGGCGACACGACGATCCCGAAAGGAACCAACATATGGATCGACATCGTAGCGATGCATCACGACCCGAGCCTTTGGGGCGATGACGTGAACGAGTTCAAGCCCGAGAGGTTCGAGAAGGACAGCATTCATGGCGGGTGCAAGCACAAGATGGGATATTTGCCATTTGGGTTTGGAGGGAGAATGTGCATTGGTAGAAACTTGTCTTCCATGGAGTATAAGATTGTGCTAACCCTAATTATTTCTAGGTTTTCTCTCTCTATTTCTCCCTCTTATTCTCATTCTCCTGCTATTTTGCTCTCTTTAAGACCTGCCCATGGCCTCCCTCTCATTCTAAAACCCCTTCACCCT

Protein sequence

VVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASASDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP

Homology

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BLAST of MC10g0127 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 9.4e-87
Identity = 188/531 (35.40%), Postives = 282/531 (53.11%), Query Frame = 0


Query: 1   VVLLVRVVFS----WWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAA 60
           V  ++RV++     +W+ P    + +++ G   GP PR   GNI EI   + ++A+    
Sbjct: 14  VTTILRVLYDTISCYWLTPRRIKKIMEQQGVT-GPKPRPLTGNILEISAMVSQSAS---- 73

Query: 61  AAINGGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNM 120
                         +I HDI   + P++  W   YGK+F+ W GT+P L + E + +K +
Sbjct: 74  ----------KDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKEL 133

Query: 121 ---SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRL 180
                 V G+ W +    +N      G GL+M  G DW   RH+  PAF    LK   R 
Sbjct: 134 LMKHNGVSGRSWLQQQGTKN----FIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARH 193

Query: 181 MVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQ 240
           MV  T+K+++   + +  G  E+++  E+     DII++  FG     G++ FN L  LQ
Sbjct: 194 MVECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQ 253

Query: 241 FTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASAS-----D 300
               + + ++  P  S FL        K L  E++RL + II  R+   +   S     D
Sbjct: 254 RRCAQATRHLCFP-GSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDD 313

Query: 301 LLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQ 360
           LL  L+     D       + L  + ++DECKTFFF GHETTAL ++WT +LLA  P WQ
Sbjct: 314 LLGLLLNEMDIDKNNNNNNNNL--QLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQ 373

Query: 361 TILREEIKEVIGDREI-DFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTI 420
             +REE++EV G   +     LS+L  +  V NE LRLYP A  + R A  D+K+GD TI
Sbjct: 374 EKVREEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTI 433

Query: 421 PKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMG--YLPFGFGGRMCI 480
           PKG +IWI ++A+HH   LWG D N+F PERF      GG     G  ++PF  G R CI
Sbjct: 434 PKGLSIWIPVLAIHHSEELWGKDANQFNPERF------GGRPFASGRHFIPFAAGPRNCI 493

Query: 481 GRNLSSMEYKIVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 517
           G+  + ME KI+L  +IS+F+ +IS +Y H+P ++L+++P +G+ +ILKPL
Sbjct: 494 GQQFALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

[Close]

BLAST of MC10g0127 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 5.1e-85
Identity = 188/529 (35.54%), Postives = 288/529 (54.44%), Query Frame = 0


Query: 1   VVLLVRVVFS----WWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAA 60
           + L++RV++     +++ P    + ++R G   GP PR   GNI +I   +  +A+    
Sbjct: 15  MTLILRVLYDSICCYFLTPRRIKKFMERQGIT-GPKPRLLTGNIIDISKMLSHSAS---- 74

Query: 61  AAINGGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNM 120
                     +  S+I H+I   + P++  W   YGK+F+ W GTEP L + E + +K +
Sbjct: 75  ----------NDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKEL 134

Query: 121 ---SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRL 180
                 V GK W    + +   +   G GL+M  G+ W   RH+  PAF    LK   + 
Sbjct: 135 LTKHNPVTGKSW----LQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKH 194

Query: 181 MVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQ 240
           MV   TKM+ E  R+    G E+++  E+     DII++  FG     G++ F+ L  LQ
Sbjct: 195 MV-ECTKMMAERLRK--EVGEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQ 254

Query: 241 FTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASAS-----D 300
               + + ++  P  S FL        K L +E++RL + II  RK S +   S     D
Sbjct: 255 RLCAQATRHLCFP-GSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDD 314

Query: 301 LLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQ 360
           LL  L+    ++      K+ L  + ++DECKTFFF GHETT+L ++WT++LLA  P WQ
Sbjct: 315 LLGLLLNQMDSN------KNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQ 374

Query: 361 TILREEIKEVIG-DREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTI 420
             +R+E+++V G D       LS L  +  V NE LRLYP A  + R A  DIK+GD  I
Sbjct: 375 DNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLII 434

Query: 421 PKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGR 480
           PKG +IWI ++A+HH   LWG+D NEF PERF   S     +H   ++PF  G R CIG+
Sbjct: 435 PKGLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSF-ASSRH---FMPFAAGPRNCIGQ 494

Query: 481 NLSSMEYKIVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 517
             + ME KI+L +++S+FS +IS +Y H+P ++L+++P +G+ L+LKPL
Sbjct: 495 TFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510

[Close]

BLAST of MC10g0127 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 7.4e-84
Identity = 185/522 (35.44%), Postives = 278/522 (53.26%), Query Frame = 0


Query: 1   VVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAIN 60
           ++L   V    W+ P    +KL+  G   GP P F  GNI E++   + A ++    A  
Sbjct: 20  ILLFSYVYIILWLRPERLRQKLRSQGVR-GPKPSFLFGNIPEMRRIQQLAKSAHEQEA-- 79

Query: 61  GGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAV 120
           G    FSS      +  +T+FPYF  W   YG  ++Y  G+   L + +P  VK ++   
Sbjct: 80  GSTDMFSS------NYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDPNMVKELANC- 139

Query: 121 MGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTK 180
                GKP   + +R  + G G++ + GD W+  R ++ P      +K M  LM+ +   
Sbjct: 140 KSLDLGKPCYLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKGMVNLMMEAAMS 199

Query: 181 MLDEWARRLS--SGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFR 240
           ML+ W   +    G  E+ V+  +   + D+I++A FG     G++ F K+R LQ  + +
Sbjct: 200 MLNSWKNEVEDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFIKIRQLQKAMAK 259

Query: 241 NSSYVGVPFSSIFLYPTRTLTA-KRLGSEIDRLFLSIISERKQSPDASAS---DLLSRLI 300
            S  +GVP S     PTR+      L S I  L L+I   +K   D+S S   DLL  +I
Sbjct: 260 QSMLIGVPGSRYL--PTRSNRGIWNLDSSIRTLILNI--SKKYEHDSSTSVNKDLLHSII 319

Query: 301 ESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREE 360
           +  ++   P A  S      +VD CK  +F GHETT+   +W ++LLA+  EWQ+  R E
Sbjct: 320 QGSKD--GPFA--SCTPEDFIVDNCKNIYFAGHETTSTTAAWCLMLLASHHEWQSRARVE 379

Query: 361 IKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIW 420
             ++   R +DF +L +LKK+  V  E LRLYP A  + R+A  D+K+G   IPKGTNIW
Sbjct: 380 SLDICQGRPLDFDILRKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGIDIPKGTNIW 439

Query: 421 IDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEY 480
           I I   H DPS+WG   ++F P+RF  + I G CK    Y+PFG G R C G+NL+ +E 
Sbjct: 440 IPIAMAHRDPSVWGPSADKFDPDRF-ANGIAGACKPPHMYMPFGVGVRTCAGQNLAMVEL 499

Query: 481 KIVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 517
           K+VL+L++S+F   +SP+Y H PA  L++ P  G+PLI + L
Sbjct: 500 KVVLSLLLSKFEFKLSPNYVHCPAFRLTIEPGKGVPLIFREL 522

[Close]

BLAST of MC10g0127 vs. ExPASy Swiss-Prot
Match: B6SSW8 (Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 7.0e-82
Identity = 171/507 (33.73%), Postives = 268/507 (52.86%), Query Frame = 0


Query: 12  WILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAINGGAGAFSSSSN 71
           W++P     K +     GGP P FP GN+ +++     A A+ A+A  +G  G       
Sbjct: 32  WLVPQKVLAKFEDQKI-GGPRPSFPYGNLADMREAAAAAKAARASARRSGSGG-----GG 91

Query: 72  ISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAVMGKGWGKPAVF 131
           I HD    V PY+ +W+  YG  F Y +G   FL+++ P  V++++  V     GK +  
Sbjct: 92  IVHDYRPAVLPYYEKWRKEYGPIFTYSMGNVVFLHVSRPDVVRDINLCV-SLDLGKSSYL 151

Query: 132 RNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTKMLDEWARRLSS 191
           +   EP+FG G++ + G+ W+  R I+ P F    +K M  LMV S   +L  W  R+  
Sbjct: 152 KATHEPLFGGGILKSNGEAWLHQRKIIAPEFFLDKVKGMVDLMVDSAQPLLMSWEERVDR 211

Query: 192 GG--PEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFRNSSYVGVPFSS 251
            G   ++ ++ +I A + D+I++  FG     G++ F K+R+LQ  + + +  V    + 
Sbjct: 212 NGGITDIKIDDDIRAYSADVISRTCFGSSYIKGKEIFMKIRELQQAVSKPN--VLAEMTG 271

Query: 252 IFLYPT-RTLTAKRLGSEIDRLFLSIISERKQSPDASASDLLSRLIESQRNDSAPAAGKS 311
           +  +P+ R   A  L  ++ +L L I+ E  +       +LLS ++ S        A   
Sbjct: 272 LRFFPSMRNKQAWELHKQVRKLILEIVKESGED-----RNLLSAILHSASTSRVGIAE-- 331

Query: 312 WLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEIKEVIGDREIDFAM 371
                 +VD CK+ +F GHE+TA+  +W ++LL   PEWQ  +REE+ EV   + +D   
Sbjct: 332 --AENFIVDNCKSIYFAGHESTAVTAAWCLMLLGLHPEWQNRVREEVHEVCRGQPVDSRS 391

Query: 372 LSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVAMHHDPSLWG 431
           L ++K +  V  E LRLYP+   + RQA  ++K+G   IPKG NI+I +  MH DP LWG
Sbjct: 392 LQKMKNLTMVIQETLRLYPAGAFVSRQALQELKLGGVHIPKGVNIYIPVSTMHLDPELWG 451

Query: 432 DDVNEFKPERFE--KDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLIISRFS 491
            DV EF PERF   +  +H        YLPFG G R C+G+  +  E KI+++LI+S+F 
Sbjct: 452 PDVKEFNPERFSDVRPQLH-------SYLPFGAGARTCLGQGFAMAELKILISLIVSKFV 511

Query: 492 LSISPSYSHSPAILLSLRPAHGLPLIL 514
           L +SP Y HSP + L + P  G+ L L
Sbjct: 512 LKLSPHYQHSPTLKLIVEPELGVDLTL 513

[Close]

BLAST of MC10g0127 vs. ExPASy Swiss-Prot
Match: Q5KQH7 (Cytochrome P450 714D1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714D1 PE=1 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 7.7e-81
Identity = 171/540 (31.67%), Postives = 276/540 (51.11%), Query Frame = 0


Query: 7   VVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAA-------AAAI 66
           +  +W   P       +R G +G PP  F  GN+ E+K  +  AA++AA        A+ 
Sbjct: 27  ITAAWLARPRRVAEVFRRQGIDGPPPSSFLAGNLPEMKARVAAAASAAAPTADGEETASA 86

Query: 67  NGGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEA 126
            GG G      +   D  + +FPYF +W+ +YG+ ++YWL   P LY+ +P+ +  +   
Sbjct: 87  GGGGGGRDFEKDGFDDYCTRIFPYFHKWRKAYGETYLYWLRRRPALYVTDPELIGEIGRC 146

Query: 127 VMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTT 186
           V     GKP   +  +EP+FG G++   G  W R R ++ P F  A ++AM +LMV +  
Sbjct: 147 V-SLDMGKPKYLQKGQEPLFGGGVLKANGACWARQRKVIAPEFYMARVRAMVQLMVDAAQ 206

Query: 187 KMLDEWARRLSSGG----PEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFT 246
            ++  W  R+ + G     E+ V+ ++ + + D+I++A FG     GR+ F +LR+L   
Sbjct: 207 PLIASWESRIDAAGGAAAAEVVVDGDLRSFSFDVISRACFGSDYSRGREIFLRLRELSGL 266

Query: 247 LFRNSSYVGVPFSSIFLYPT-RTLTAKRLGSEIDRLFLSIISERK-------------QS 306
           +   S    +P  S+   PT +     RL  EI  L + ++ ER+               
Sbjct: 267 MSETSVIFSIP--SLRHLPTGKNRRIWRLTGEIRSLIMELVRERRCAARAAREHGGKAAP 326

Query: 307 PDASASDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLL 366
           P     D L  +IE+      P           +VD CK  +F GHET+A+  +W ++LL
Sbjct: 327 PSPPERDFLGSIIENSGGQPRP--------DDFVVDNCKNIYFAGHETSAVTATWCLMLL 386

Query: 367 ATRPEWQTILREEIKEVIG------DREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQ 426
           A  PEWQ   R E+ EV G          DF M+SR++ +G V  E LRL+P +  + R+
Sbjct: 387 AAHPEWQDRARAEVLEVCGGDGAAAPAAPDFDMVSRMRTVGMVVQETLRLFPPSSFVVRE 446

Query: 427 AKGDIKVGDTTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKH-KMGY 486
              D+++G    PKGT +++ +  MHHD + WG     F P RF +D +   CKH +  +
Sbjct: 447 TFRDMQLGRLLAPKGTYLFVPVSTMHHDVAAWGPTARLFDPSRF-RDGVAAACKHPQASF 506

Query: 487 LPFGFGGRMCIGRNLSSMEYKIVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILK 515
           +PFG G R C+G+NL+ +E K ++ ++++RF  ++SP Y HSPA  L + P  GL L ++
Sbjct: 507 MPFGLGARTCLGQNLALVEVKTLVAVVLARFEFTLSPEYRHSPAFRLIIEPEFGLRLRIR 554

[Close]

BLAST of MC10g0127 vs. NCBI nr
Match: XP_022141037.1 (cytokinin hydroxylase [Momordica charantia])

HSP 1 Score: 1042 bits (2695), Expect = 0.0
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 0


Query: 1   VVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAIN 60
           VVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAIN
Sbjct: 17  VVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAIN 76

Query: 61  GGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAV 120
           GGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAV
Sbjct: 77  GGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAV 136

Query: 121 MGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTK 180
           MGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTK
Sbjct: 137 MGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTK 196

Query: 181 MLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFRNS 240
           MLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFRNS
Sbjct: 197 MLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFRNS 256

Query: 241 SYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASASDLLSRLIESQRND 300
           SYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASASDLLSRLIESQRND
Sbjct: 257 SYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASASDLLSRLIESQRND 316

Query: 301 SAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEIKEVIG 360
           SAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEIKEVIG
Sbjct: 317 SAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEIKEVIG 376

Query: 361 DREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVAM 420
           DREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVAM
Sbjct: 377 DREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVAM 436

Query: 421 HHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTL 480
           HHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTL
Sbjct: 437 HHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTL 496

Query: 481 IISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 518
           IISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP
Sbjct: 497 IISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 534

[Close]

BLAST of MC10g0127 vs. NCBI nr
Match: KAG6574181.1 (Cytochrome P450 714C2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 811 bits (2095), Expect = 1.74e-292
Identity = 407/517 (78.72%), Postives = 450/517 (87.04%), Query Frame = 0


Query: 2   VLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAING 61
           VL V+VV SWW+LPVLAH+KLKRNGF G PPP FPLGNI E+K   K      A+AAING
Sbjct: 18  VLFVKVVVSWWVLPVLAHQKLKRNGFRG-PPPSFPLGNIAEMKRIRK------ASAAING 77

Query: 62  GAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAVM 121
            A +F SS  +SHDIHSTVFP+FAQWQ SYGKKF+YWLGTEPFLYIA+P F+K +SEAVM
Sbjct: 78  DAHSFPSS-KVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYIADPHFMKKISEAVM 137

Query: 122 GKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTKM 181
            KGWGKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANLKAMT+ MV STTKM
Sbjct: 138 AKGWGKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANLKAMTKFMVESTTKM 197

Query: 182 LDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFRNSS 241
           L++W+  ++S G E+DVEREITATAGDIIAKASFGIC+  GRDAF+ LR LQFTLF+NS+
Sbjct: 198 LNKWSSLVNSAGGEIDVEREITATAGDIIAKASFGICNDGGRDAFDYLRALQFTLFQNST 257

Query: 242 YVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASASDLLSRLIESQRNDS 301
           YVGVPFSS+F  P RTL+AKRLG+ IDRLFLS++S+RK S D+S SDLLSRLIE ++  S
Sbjct: 258 YVGVPFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQDSS-SDLLSRLIEGEK--S 317

Query: 302 APAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEIKEVIGD 361
               GK  LTTRELVDECKTFFFGGHETTALA SWT+LLLATRPEWQ ILREEIKEVIGD
Sbjct: 318 TGDGGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGD 377

Query: 362 REIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVAMH 421
           +EIDF MLS LKKMG VF+EVLRLYPSAPNIQRQAKGDI VG  TIPKGTNIWIDIVAMH
Sbjct: 378 KEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKGTNIWIDIVAMH 437

Query: 422 HDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLI 481
            DP LWGDD NEFKPERF +DSI GGC HKMGYLPFGFGGRMCIG+NLSSMEYKIVLTLI
Sbjct: 438 RDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLSSMEYKIVLTLI 497

Query: 482 ISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 518
           +SRFSLS+SP+Y+HSPA LLSLRPAHGLPLI+ PLHP
Sbjct: 498 LSRFSLSLSPNYAHSPATLLSLRPAHGLPLIVTPLHP 523

[Close]

BLAST of MC10g0127 vs. NCBI nr
Match: XP_022945852.1 (cytochrome P450 714C2-like [Cucurbita moschata])

HSP 1 Score: 810 bits (2091), Expect = 7.06e-292
Identity = 406/517 (78.53%), Postives = 450/517 (87.04%), Query Frame = 0


Query: 2   VLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAING 61
           VL V+VV SWW+LPVLAH+KLKRNGF G PPP FPLGNI E+K   K      A+AAING
Sbjct: 18  VLFVKVVVSWWVLPVLAHQKLKRNGFRG-PPPSFPLGNIAEMKRIRK------ASAAING 77

Query: 62  GAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAVM 121
            A +F SS  +SHDIHSTVFP+FAQWQ SYGKKF+YWLGTEPFLYIA+P F+K +SEAVM
Sbjct: 78  DAHSFPSS-KVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYIADPHFMKKISEAVM 137

Query: 122 GKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTKM 181
            KGWGKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANLKAMT+ MV STTKM
Sbjct: 138 AKGWGKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANLKAMTKFMVESTTKM 197

Query: 182 LDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFRNSS 241
           L++W+  ++S G E+DVEREITATAGDIIA+ASFGIC+  GRDAF+ LR LQFTLF+NS+
Sbjct: 198 LNKWSSLVNSAGGEIDVEREITATAGDIIAEASFGICNDGGRDAFDYLRALQFTLFQNST 257

Query: 242 YVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASASDLLSRLIESQRNDS 301
           YVGVPFSS+F  P RTL+AKRLG+ IDRLFLS++S+RK S D+S SDLLSRLIE ++  S
Sbjct: 258 YVGVPFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQDSS-SDLLSRLIEGEK--S 317

Query: 302 APAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEIKEVIGD 361
               GK  LTTRELVDECKTFFFGGHETTALA SWT+LLLATRPEWQ ILREEIKEVIGD
Sbjct: 318 TGDGGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGD 377

Query: 362 REIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVAMH 421
           +EIDF MLS LKKMG VF+EVLRLYPSAPNIQRQAKGDI VG  TIPKGTNIWIDIVAMH
Sbjct: 378 KEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKGTNIWIDIVAMH 437

Query: 422 HDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLI 481
            DP LWGDD NEFKPERF +DSI GGC HKMGYLPFGFGGRMCIG+NLSSMEYKIVLTLI
Sbjct: 438 RDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLSSMEYKIVLTLI 497

Query: 482 ISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 518
           +SRFSLS+SP+Y+HSPA LLSLRPAHGLPLI+ PLHP
Sbjct: 498 LSRFSLSLSPNYAHSPATLLSLRPAHGLPLIVTPLHP 523

[Close]

BLAST of MC10g0127 vs. NCBI nr
Match: XP_038877847.1 (cytokinin hydroxylase-like [Benincasa hispida])

HSP 1 Score: 805 bits (2079), Expect = 5.92e-290
Identity = 405/516 (78.49%), Postives = 444/516 (86.05%), Query Frame = 0


Query: 6   RVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAINGGAGA 65
           +VV SWWILP+LAH+ LKRNGF G P P FPLGNITE+K  +K      AAAAINGG+ +
Sbjct: 25  KVVVSWWILPILAHKNLKRNGFEG-PSPTFPLGNITEMKKIIK------AAAAINGGS-S 84

Query: 66  FSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAVMGKGW 125
            +SS+N+SHDIHST+FP+FAQWQ SYGKKFVYWLGTEPFLYIA+PQ VK +SEAVMGK W
Sbjct: 85  LASSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVMGKSW 144

Query: 126 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTKMLDEW 185
           GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNP NLKAMT+ M+ STTKM+ EW
Sbjct: 145 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPTNLKAMTKFMLESTTKMVKEW 204

Query: 186 ARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSG-RDAFNKLRDLQFTLFRNSSYVG 245
              ++SG  E+DVEREITATAGDIIAKASFGI    G  +A N LR LQ TLF NSSYVG
Sbjct: 205 RSLVNSGQSEIDVEREITATAGDIIAKASFGISSGGGGEEALNSLRALQLTLFSNSSYVG 264

Query: 246 VPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERK--QSPDASASDLLSRLIESQRNDSA 305
           VPFSS+FLYP RTL AKRLG++IDRLF SIIS RK   S   S SDLLSRLI+SQ N   
Sbjct: 265 VPFSSLFLYPGRTLAAKRLGAQIDRLFFSIISHRKLISSGSDSDSDLLSRLIQSQSNK-- 324

Query: 306 PAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEIKEVIGDR 365
              G + LT RE+VDECKTFFFGGHETTALAISWT+LLLATRPEWQ ILREEIKEVIGD+
Sbjct: 325 -YKGGAALTPREVVDECKTFFFGGHETTALAISWTLLLLATRPEWQKILREEIKEVIGDK 384

Query: 366 EIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVAMHH 425
           +IDF+MLS LKKMGWV+NEVLRLYPSAPNIQRQAKGDI +GD  IPKGTNIWIDIVAMHH
Sbjct: 385 DIDFSMLSALKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVAMHH 444

Query: 426 DPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLII 485
           DP+LWGDDVNEFKPERF+ D+IHGGC HKMGYLPFGFGGRMCIGRNLSS EYKIVLTLI+
Sbjct: 445 DPTLWGDDVNEFKPERFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSTEYKIVLTLIL 504

Query: 486 SRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 518
           S FSLS+SPSYSHSPAILLSLRP HG+PLIL PLHP
Sbjct: 505 STFSLSLSPSYSHSPAILLSLRPTHGIPLILTPLHP 529

[Close]

BLAST of MC10g0127 vs. NCBI nr
Match: XP_022968014.1 (cytochrome P450 714C2-like [Cucurbita maxima])

HSP 1 Score: 804 bits (2076), Expect = 1.35e-289
Identity = 406/517 (78.53%), Postives = 447/517 (86.46%), Query Frame = 0


Query: 2   VLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAING 61
           VL V+VV SWW+LPVLAH+KLKRNGF G PPP FPLGNI E+K   K      A+AAING
Sbjct: 18  VLFVKVVVSWWVLPVLAHQKLKRNGFRG-PPPTFPLGNIAEMKRIRK------ASAAING 77

Query: 62  GAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAVM 121
            + +F SS  +SHDIHSTVFP+FAQWQ SYGKKFVYWLGTEPFLYIA+P F+K +S AVM
Sbjct: 78  DSHSFPSS-KVSHDIHSTVFPHFAQWQASYGKKFVYWLGTEPFLYIADPHFMKQISVAVM 137

Query: 122 GKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTKM 181
            KGWGKPAVFRNDR PMFGDGLVMTEGD W+RHRHILTPAFNPANLKAMT+ MV STTKM
Sbjct: 138 AKGWGKPAVFRNDRRPMFGDGLVMTEGDVWVRHRHILTPAFNPANLKAMTKFMVESTTKM 197

Query: 182 LDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFRNSS 241
           L+EW+  ++S   E+DVEREITATAGDIIAKASFGIC+  GRDAF+ LR LQFTLF+NS+
Sbjct: 198 LNEWSSLVNSASGEIDVEREITATAGDIIAKASFGICNGGGRDAFDYLRALQFTLFQNST 257

Query: 242 YVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASASDLLSRLIESQRNDS 301
           YVGVPFSS+F  P RTL AKRLG+ IDRLFLS++SERK S ++S SDLLSRLIE Q+  S
Sbjct: 258 YVGVPFSSLFFCPGRTLAAKRLGAAIDRLFLSLVSERKLSQNSS-SDLLSRLIEGQK--S 317

Query: 302 APAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEIKEVIGD 361
               GK  LTTRELVDECKTFFFGGHETTALA SWT+LLLATRPEWQ ILREEIKEVIGD
Sbjct: 318 TGDVGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGD 377

Query: 362 REIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVAMH 421
           +EIDF MLS LKKMG VF+EVLRLYPSAPNIQRQAKGDI+VG  TIPKGTNIWIDIVAMH
Sbjct: 378 KEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIEVGSMTIPKGTNIWIDIVAMH 437

Query: 422 HDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLI 481
            DP LWGDD NEFKPERF +DSI GGC HKMGYLPFGFGGRMCIG++LSSMEYKIVLTLI
Sbjct: 438 RDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKSLSSMEYKIVLTLI 497

Query: 482 ISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 518
           +SRFSLS+SP+YSHSPA LLSLRPAHGLPLI+ PLHP
Sbjct: 498 LSRFSLSLSPNYSHSPATLLSLRPAHGLPLIVTPLHP 523

[Close]

BLAST of MC10g0127 vs. ExPASy TrEMBL
Match: A0A6J1CIQ8 (cytokinin hydroxylase OS=Momordica charantia OX=3673 GN=LOC111011538 PE=3 SV=1)

HSP 1 Score: 1042 bits (2695), Expect = 0.0
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 0


Query: 1   VVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAIN 60
           VVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAIN
Sbjct: 17  VVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAIN 76

Query: 61  GGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAV 120
           GGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAV
Sbjct: 77  GGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAV 136

Query: 121 MGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTK 180
           MGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTK
Sbjct: 137 MGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTK 196

Query: 181 MLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFRNS 240
           MLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFRNS
Sbjct: 197 MLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFRNS 256

Query: 241 SYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASASDLLSRLIESQRND 300
           SYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASASDLLSRLIESQRND
Sbjct: 257 SYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASASDLLSRLIESQRND 316

Query: 301 SAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEIKEVIG 360
           SAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEIKEVIG
Sbjct: 317 SAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEIKEVIG 376

Query: 361 DREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVAM 420
           DREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVAM
Sbjct: 377 DREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVAM 436

Query: 421 HHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTL 480
           HHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTL
Sbjct: 437 HHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTL 496

Query: 481 IISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 518
           IISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP
Sbjct: 497 IISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 534

[Close]

BLAST of MC10g0127 vs. ExPASy TrEMBL
Match: A0A6J1G224 (cytochrome P450 714C2-like OS=Cucurbita moschata OX=3662 GN=LOC111449972 PE=3 SV=1)

HSP 1 Score: 810 bits (2091), Expect = 3.42e-292
Identity = 406/517 (78.53%), Postives = 450/517 (87.04%), Query Frame = 0


Query: 2   VLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAING 61
           VL V+VV SWW+LPVLAH+KLKRNGF G PPP FPLGNI E+K   K      A+AAING
Sbjct: 18  VLFVKVVVSWWVLPVLAHQKLKRNGFRG-PPPSFPLGNIAEMKRIRK------ASAAING 77

Query: 62  GAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAVM 121
            A +F SS  +SHDIHSTVFP+FAQWQ SYGKKF+YWLGTEPFLYIA+P F+K +SEAVM
Sbjct: 78  DAHSFPSS-KVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYIADPHFMKKISEAVM 137

Query: 122 GKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTKM 181
            KGWGKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANLKAMT+ MV STTKM
Sbjct: 138 AKGWGKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANLKAMTKFMVESTTKM 197

Query: 182 LDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFRNSS 241
           L++W+  ++S G E+DVEREITATAGDIIA+ASFGIC+  GRDAF+ LR LQFTLF+NS+
Sbjct: 198 LNKWSSLVNSAGGEIDVEREITATAGDIIAEASFGICNDGGRDAFDYLRALQFTLFQNST 257

Query: 242 YVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASASDLLSRLIESQRNDS 301
           YVGVPFSS+F  P RTL+AKRLG+ IDRLFLS++S+RK S D+S SDLLSRLIE ++  S
Sbjct: 258 YVGVPFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQDSS-SDLLSRLIEGEK--S 317

Query: 302 APAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEIKEVIGD 361
               GK  LTTRELVDECKTFFFGGHETTALA SWT+LLLATRPEWQ ILREEIKEVIGD
Sbjct: 318 TGDGGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGD 377

Query: 362 REIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVAMH 421
           +EIDF MLS LKKMG VF+EVLRLYPSAPNIQRQAKGDI VG  TIPKGTNIWIDIVAMH
Sbjct: 378 KEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKGTNIWIDIVAMH 437

Query: 422 HDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLI 481
            DP LWGDD NEFKPERF +DSI GGC HKMGYLPFGFGGRMCIG+NLSSMEYKIVLTLI
Sbjct: 438 RDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLSSMEYKIVLTLI 497

Query: 482 ISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 518
           +SRFSLS+SP+Y+HSPA LLSLRPAHGLPLI+ PLHP
Sbjct: 498 LSRFSLSLSPNYAHSPATLLSLRPAHGLPLIVTPLHP 523

[Close]

BLAST of MC10g0127 vs. ExPASy TrEMBL
Match: A0A6J1HYD5 (cytochrome P450 714C2-like OS=Cucurbita maxima OX=3661 GN=LOC111467387 PE=3 SV=1)

HSP 1 Score: 804 bits (2076), Expect = 6.56e-290
Identity = 406/517 (78.53%), Postives = 447/517 (86.46%), Query Frame = 0


Query: 2   VLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAING 61
           VL V+VV SWW+LPVLAH+KLKRNGF G PPP FPLGNI E+K   K      A+AAING
Sbjct: 18  VLFVKVVVSWWVLPVLAHQKLKRNGFRG-PPPTFPLGNIAEMKRIRK------ASAAING 77

Query: 62  GAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAVM 121
            + +F SS  +SHDIHSTVFP+FAQWQ SYGKKFVYWLGTEPFLYIA+P F+K +S AVM
Sbjct: 78  DSHSFPSS-KVSHDIHSTVFPHFAQWQASYGKKFVYWLGTEPFLYIADPHFMKQISVAVM 137

Query: 122 GKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTKM 181
            KGWGKPAVFRNDR PMFGDGLVMTEGD W+RHRHILTPAFNPANLKAMT+ MV STTKM
Sbjct: 138 AKGWGKPAVFRNDRRPMFGDGLVMTEGDVWVRHRHILTPAFNPANLKAMTKFMVESTTKM 197

Query: 182 LDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFRNSS 241
           L+EW+  ++S   E+DVEREITATAGDIIAKASFGIC+  GRDAF+ LR LQFTLF+NS+
Sbjct: 198 LNEWSSLVNSASGEIDVEREITATAGDIIAKASFGICNGGGRDAFDYLRALQFTLFQNST 257

Query: 242 YVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASASDLLSRLIESQRNDS 301
           YVGVPFSS+F  P RTL AKRLG+ IDRLFLS++SERK S ++S SDLLSRLIE Q+  S
Sbjct: 258 YVGVPFSSLFFCPGRTLAAKRLGAAIDRLFLSLVSERKLSQNSS-SDLLSRLIEGQK--S 317

Query: 302 APAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEIKEVIGD 361
               GK  LTTRELVDECKTFFFGGHETTALA SWT+LLLATRPEWQ ILREEIKEVIGD
Sbjct: 318 TGDVGKMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGD 377

Query: 362 REIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVAMH 421
           +EIDF MLS LKKMG VF+EVLRLYPSAPNIQRQAKGDI+VG  TIPKGTNIWIDIVAMH
Sbjct: 378 KEIDFNMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIEVGSMTIPKGTNIWIDIVAMH 437

Query: 422 HDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLI 481
            DP LWGDD NEFKPERF +DSI GGC HKMGYLPFGFGGRMCIG++LSSMEYKIVLTLI
Sbjct: 438 RDPDLWGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKSLSSMEYKIVLTLI 497

Query: 482 ISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 518
           +SRFSLS+SP+YSHSPA LLSLRPAHGLPLI+ PLHP
Sbjct: 498 LSRFSLSLSPNYSHSPATLLSLRPAHGLPLIVTPLHP 523

[Close]

BLAST of MC10g0127 vs. ExPASy TrEMBL
Match: A0A1S3AXX2 (cytokinin hydroxylase OS=Cucumis melo OX=3656 GN=LOC103484084 PE=3 SV=1)

HSP 1 Score: 786 bits (2029), Expect = 1.00e-282
Identity = 397/519 (76.49%), Postives = 441/519 (84.97%), Query Frame = 0


Query: 2   VLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAING 61
           VL  +VV S WILP+LAHRKLKRNGF G P P FPLGNITE+K  MK  AA+AA A  NG
Sbjct: 21  VLFGKVVISCWILPILAHRKLKRNGFEG-PSPSFPLGNITEMKKIMK--AAAAATAVTNG 80

Query: 62  GAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAVM 121
                SSS+N+SHDIHST+FP+FAQWQ SYGKKFVYWLGTEPFLYIA+PQ VK +SEAV+
Sbjct: 81  -----SSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVL 140

Query: 122 GKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTKM 181
           GK WGKPAVFRNDREPMFGDGLVMTEGD+WIRHRHILTPAFNP NLKAMT+ MV STTKM
Sbjct: 141 GKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKAMTKFMVESTTKM 200

Query: 182 LDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICD-RSGRDAFNKLRDLQFTLFRNS 241
           L+EW   + SG  E++VER+ITATAG+IIAKASFGI +   G +A N LR LQ TLF+NS
Sbjct: 201 LEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNNLRALQLTLFKNS 260

Query: 242 SYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASA-SDLLSRLIESQRN 301
           SYVGVPFSS+FLYP RTL AKRLG++IDRLFLSIIS RK S   S  SDLLSRLI S+  
Sbjct: 261 SYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYSDLLSRLIGSEYT 320

Query: 302 DSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEIKEVI 361
                 G   L+ RE+VDECKTFFFGGHETTALAISWT+LLLAT P+WQ ILREEIKEV+
Sbjct: 321 ------GGEALSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILREEIKEVV 380

Query: 362 GDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIVA 421
           GD++IDF+MLS LKKMGWV+NEVLRLYPSAPNIQRQAKGDI +GD  IPKGTNIWID+VA
Sbjct: 381 GDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNIWIDVVA 440

Query: 422 MHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVLT 481
           MHHDP+LWGDDVN+F P RF+ D+IHGGC HKMGYLPFGFGGRMCIGRNLSSMEYKIVLT
Sbjct: 441 MHHDPTLWGDDVNDFNPSRFQHDTIHGGCYHKMGYLPFGFGGRMCIGRNLSSMEYKIVLT 500

Query: 482 LIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 518
           LI+S FSLS+SPSYSHSPA LLSLRPAHG+PLIL PL P
Sbjct: 501 LILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 525

[Close]

BLAST of MC10g0127 vs. ExPASy TrEMBL
Match: A0A5D3DI76 (Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00640 PE=3 SV=1)

HSP 1 Score: 728 bits (1878), Expect = 1.03e-260
Identity = 366/478 (76.57%), Postives = 408/478 (85.36%), Query Frame = 0


Query: 43  IKNTMKKAAASAAAAAINGGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTE 102
           +K  MK  AA+AA A  NG     SSS+N+SHDIHST+FP+FAQWQ SYGKKFVYWLGTE
Sbjct: 1   MKKIMK--AAAAATAVTNG-----SSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTE 60

Query: 103 PFLYIAEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAF 162
           PFLYIA+PQ VK +SEAV+GK WGKPAVFRNDREPMFGDGLVMTEGD+WIRHRHILTPAF
Sbjct: 61  PFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAF 120

Query: 163 NPANLKAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICD-RS 222
           NP NLKAMT+ MV STTKML+EW   + SG  E++VER+ITATAG+IIAKASFGI +   
Sbjct: 121 NPTNLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGG 180

Query: 223 GRDAFNKLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQS 282
           G +A N LR LQ TLF+NSSYVGVPFSS+FLYP RTL AKRLG++IDRLFLSIIS RK S
Sbjct: 181 GGEALNNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMS 240

Query: 283 PDASA-SDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLL 342
              S  SDLLSRLI S+        G   L+ RE+VDECKTFFFGGHETTALAISWT+LL
Sbjct: 241 GSGSGYSDLLSRLIGSEYT------GGEALSPREVVDECKTFFFGGHETTALAISWTLLL 300

Query: 343 LATRPEWQTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDI 402
           LAT P+WQ ILREEIKEV+GD++IDF+MLS LKKMGWV+NEVLRLYPSAPNIQRQAKGDI
Sbjct: 301 LATSPDWQRILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDI 360

Query: 403 KVGDTTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFG 462
            +GD  IPKGTNIWID+VAMHHDP+LWGDDVN+F P RF+ D+IHGGC HKMGYLPFGFG
Sbjct: 361 DLGDIKIPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFG 420

Query: 463 GRMCIGRNLSSMEYKIVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 518
           GRMCIGRNLSSMEYKIVLTLI+S FSLS+SPSYSHSPA LLSLRPAHG+PLIL PL P
Sbjct: 421 GRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 465

[Close]

BLAST of MC10g0127 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 607.8 bits (1566), Expect = 7.9e-174
Identity = 301/518 (58.11%), Postives = 375/518 (72.39%), Query Frame = 0


Query: 2   VLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAING 61
           ++ +++    WI PV A +KL+ NGF  GP P FP GN+    N MKK     A+  ++ 
Sbjct: 23  LMFLKLFLRCWIWPVRAQKKLRGNGF-VGPAPSFPFGNL----NDMKK--LKMASVVVDN 82

Query: 62  GAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAVM 121
                 SS+ I+HDIHS   P+FA+WQ  YGK FVYWLG EPF+Y+A+P+F+  MS+ V+
Sbjct: 83  S----KSSTIINHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYVADPEFLSVMSKGVL 142

Query: 122 GKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTKM 181
           GK WGKP VF+ DREPMFG GLVM EGDDW RHRHI+TPAF P NLK MT +MV S + M
Sbjct: 143 GKSWGKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLKVMTNMMVESVSNM 202

Query: 182 LDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTLFRNSS 241
           LD W  +++SG PE D+E EI  TAG+IIAK SFG+   +G      LR +QF LF ++ 
Sbjct: 203 LDRWGIQINSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLKNLRAVQFALFNSNR 262

Query: 242 YVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQS---PDASASDLLSRLIESQR 301
           YVGVPFS+I  Y  +T+ AK LG EID L LS I++RK S    D    DLL  L+++ +
Sbjct: 263 YVGVPFSNILSY-KQTVKAKGLGHEIDGLLLSFINKRKISLAEGDDQGHDLLGMLLKADQ 322

Query: 302 NDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEIKEV 361
                   K   T +ELVDECKTFFF GHETTALA++WT +LLA  PEWQ  +REEI+EV
Sbjct: 323 --------KGNFTAKELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQDTIREEIREV 382

Query: 362 IGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWIDIV 421
           IGD +I++  L+ LKKM WV NEVLRLYP APN QRQA+ DI+V    IP GTNIWID+V
Sbjct: 383 IGDSKIEYNKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGRVIPNGTNIWIDVV 442

Query: 422 AMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYKIVL 481
           AMHHD  LWGDDVNEFKPERF+  ++HGGCK+KMGY+PFGFGGRMCIGRNL++MEYKIVL
Sbjct: 443 AMHHDVELWGDDVNEFKPERFD-GNLHGGCKNKMGYMPFGFGGRMCIGRNLTTMEYKIVL 502

Query: 482 TLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 517
           +L++SRF +S+SP Y HSP  +LSLRP +GLPLI++PL
Sbjct: 503 SLVLSRFEISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519

[Close]

BLAST of MC10g0127 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 322.4 bits (825), Expect = 6.7e-88
Identity = 188/531 (35.40%), Postives = 282/531 (53.11%), Query Frame = 0


Query: 1   VVLLVRVVFS----WWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAA 60
           V  ++RV++     +W+ P    + +++ G   GP PR   GNI EI   + ++A+    
Sbjct: 14  VTTILRVLYDTISCYWLTPRRIKKIMEQQGVT-GPKPRPLTGNILEISAMVSQSAS---- 73

Query: 61  AAINGGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNM 120
                         +I HDI   + P++  W   YGK+F+ W GT+P L + E + +K +
Sbjct: 74  ----------KDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKEL 133

Query: 121 ---SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRL 180
                 V G+ W +    +N      G GL+M  G DW   RH+  PAF    LK   R 
Sbjct: 134 LMKHNGVSGRSWLQQQGTKN----FIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARH 193

Query: 181 MVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQ 240
           MV  T+K+++   + +  G  E+++  E+     DII++  FG     G++ FN L  LQ
Sbjct: 194 MVECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQ 253

Query: 241 FTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASAS-----D 300
               + + ++  P  S FL        K L  E++RL + II  R+   +   S     D
Sbjct: 254 RRCAQATRHLCFP-GSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDD 313

Query: 301 LLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQ 360
           LL  L+     D       + L  + ++DECKTFFF GHETTAL ++WT +LLA  P WQ
Sbjct: 314 LLGLLLNEMDIDKNNNNNNNNL--QLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQ 373

Query: 361 TILREEIKEVIGDREI-DFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTI 420
             +REE++EV G   +     LS+L  +  V NE LRLYP A  + R A  D+K+GD TI
Sbjct: 374 EKVREEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTI 433

Query: 421 PKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMG--YLPFGFGGRMCI 480
           PKG +IWI ++A+HH   LWG D N+F PERF      GG     G  ++PF  G R CI
Sbjct: 434 PKGLSIWIPVLAIHHSEELWGKDANQFNPERF------GGRPFASGRHFIPFAAGPRNCI 493

Query: 481 GRNLSSMEYKIVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 517
           G+  + ME KI+L  +IS+F+ +IS +Y H+P ++L+++P +G+ +ILKPL
Sbjct: 494 GQQFALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

[Close]

BLAST of MC10g0127 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 316.6 bits (810), Expect = 3.7e-86
Identity = 188/529 (35.54%), Postives = 288/529 (54.44%), Query Frame = 0


Query: 1   VVLLVRVVFS----WWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAA 60
           + L++RV++     +++ P    + ++R G   GP PR   GNI +I   +  +A+    
Sbjct: 15  MTLILRVLYDSICCYFLTPRRIKKFMERQGIT-GPKPRLLTGNIIDISKMLSHSAS---- 74

Query: 61  AAINGGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNM 120
                     +  S+I H+I   + P++  W   YGK+F+ W GTEP L + E + +K +
Sbjct: 75  ----------NDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKEL 134

Query: 121 ---SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRL 180
                 V GK W    + +   +   G GL+M  G+ W   RH+  PAF    LK   + 
Sbjct: 135 LTKHNPVTGKSW----LQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKH 194

Query: 181 MVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQ 240
           MV   TKM+ E  R+    G E+++  E+     DII++  FG     G++ F+ L  LQ
Sbjct: 195 MV-ECTKMMAERLRK--EVGEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQ 254

Query: 241 FTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPDASAS-----D 300
               + + ++  P  S FL        K L +E++RL + II  RK S +   S     D
Sbjct: 255 RLCAQATRHLCFP-GSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDD 314

Query: 301 LLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQ 360
           LL  L+    ++      K+ L  + ++DECKTFFF GHETT+L ++WT++LLA  P WQ
Sbjct: 315 LLGLLLNQMDSN------KNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQ 374

Query: 361 TILREEIKEVIG-DREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTI 420
             +R+E+++V G D       LS L  +  V NE LRLYP A  + R A  DIK+GD  I
Sbjct: 375 DNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLII 434

Query: 421 PKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGR 480
           PKG +IWI ++A+HH   LWG+D NEF PERF   S     +H   ++PF  G R CIG+
Sbjct: 435 PKGLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSF-ASSRH---FMPFAAGPRNCIGQ 494

Query: 481 NLSSMEYKIVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 517
             + ME KI+L +++S+FS +IS +Y H+P ++L+++P +G+ L+LKPL
Sbjct: 495 TFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510

[Close]

BLAST of MC10g0127 vs. TAIR 10
Match: AT5G24900.1 (cytochrome P450, family 714, subfamily A, polypeptide 2 )

HSP 1 Score: 302.0 bits (772), Expect = 9.3e-82
Identity = 171/514 (33.27%), Postives = 262/514 (50.97%), Query Frame = 0


Query: 6   RVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAINGGAGA 65
           R V   W +     R LK  G   GPPP    GN++E++              I   A  
Sbjct: 30  RAVVEQWRM----RRSLKLQGVK-GPPPSIFNGNVSEMQR-------------IQSEAKH 89

Query: 66  FSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAVMGKGW 125
            S  + ISHD  S++FP+F  W+  YG+ + Y  G +  LYI  P+ VK +S+       
Sbjct: 90  CSGDNIISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQ-TNTLNL 149

Query: 126 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVGSTTKMLDEW 185
           G+         P+ G+G++ + G  W   R I+   F    +K M  LMV S   ML++W
Sbjct: 150 GRITHITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPMLNKW 209

Query: 186 ARRLSSGGP---EMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDL------QFTL 245
              +  GG    ++ V+ ++   + D+IAKA FG     G+  F+ +RDL      +  L
Sbjct: 210 EEMVKRGGEMGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDLLTAITKRSVL 269

Query: 246 FRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERK-QSPDASASDLLSRLIE 305
           FR + +  + F S        +    L  E++      + ER+ +  D    DL+  ++E
Sbjct: 270 FRFNGFTDMVFGS---KKHGDVDIDALEMELESSIWETVKEREIECKDTHKKDLMQLILE 329

Query: 306 SQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEI 365
                            R +VD CK+ +F GH++TA+++SW ++LLA  P WQ  +R+EI
Sbjct: 330 GAMRSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIRDEI 389

Query: 366 KEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWI 425
                +   D   +  LK +  V  E +RLYP AP + R+A  DI++GD  +PKG  IW 
Sbjct: 390 LSSCKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVCIWT 449

Query: 426 DIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYK 485
            I A+H DP +WG D N+FKPERF  + I   CK+   Y+PFG G R C+G+N   ME K
Sbjct: 450 LIPALHRDPEIWGPDANDFKPERF-SEGISKACKYPQSYIPFGLGPRTCVGKNFGMMEVK 509

Query: 486 IVLTLIISRFSLSISPSYSHSPAILLSLRPAHGL 510
           ++++LI+S+FS ++SP+Y HSP+  L + P HG+
Sbjct: 510 VLVSLIVSKFSFTLSPTYQHSPSHKLLVEPQHGV 520

[Close]

BLAST of MC10g0127 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-79
Identity = 174/538 (32.34%), Postives = 284/538 (52.79%), Query Frame = 0


Query: 1   VVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAAAAIN 60
           +V++  +   WW  P        + G   GPP  F +GN+ E+   M KA+         
Sbjct: 22  LVIVKGMSLLWW-RPRKIEEHFSKQGIR-GPPYHFFIGNVKELVGMMLKAS--------- 81

Query: 61  GGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNMSEAV 120
                 S     SH+I   V  ++  W+  YG  F+ W G    L +A+P  ++ +    
Sbjct: 82  ------SHPMPFSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREI---- 141

Query: 121 MGKGWGKPAVF-RNDREPMF----GDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMV 180
               + K   + +N+  P+     GDGL+  +G+ W  HR I++P F+  NLK +  +++
Sbjct: 142 ----FSKSEFYEKNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVL 201

Query: 181 GSTTKMLDEWARRLSSGGP-EMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQF 240
            S T M+D+W+ +LS  G  E+DV         D+I++ +FG     GR  F +L+  Q 
Sbjct: 202 KSVTDMVDKWSDKLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVF-RLQAQQM 261

Query: 241 TLFRNSSYVGVPFSSIFL-----YPTR-TLTAKRLGSEIDRLFLSIISERKQS------- 300
            L   +      F  +F+     +PTR  L + +L  EI +  L +I  R+Q+       
Sbjct: 262 LLCAEA------FQKVFIPGYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGE 321

Query: 301 --PDASASDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTML 360
              + +A DLL  +I+++            +T +++V+ECK+FFF G +TT+  ++WT +
Sbjct: 322 ECKEPAAKDLLGLMIQAKN-----------VTVQDIVEECKSFFFAGKQTTSNLLTWTTI 381

Query: 361 LLATRPEWQTILREEIKEVIGDREIDFA-MLSRLKKMGWVFNEVLRLYPSAPNIQRQAKG 420
           LL+  PEWQ   R+E+  V G R++     + +LK +  + NE LRLYP      R+AK 
Sbjct: 382 LLSMHPEWQAKARDEVLRVCGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKS 441

Query: 421 DIKVGDTTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFG 480
           D+K+G   IP GT + I I+A+HHD ++WG+DVNEF P RF  D +    KH +G++PFG
Sbjct: 442 DVKLGGYKIPCGTELLIPIIAVHHDQAIWGNDVNEFNPARF-ADGVPRAAKHPVGFIPFG 501

Query: 481 FGGRMCIGRNLSSMEYKIVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPL 517
            G R CIG+NL+ ++ K+ L ++I RF+  ++P+Y H+P +L+ L P HG P+  + L
Sbjct: 502 LGVRTCIGQNLAILQAKLTLAVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRL 515

The following BLAST results are available for this feature:

BLAST of MC10g0127 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FF18	9.4e-87	35.40	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1	[more]
Q9ZW95	5.1e-85	35.54	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1	[more]
Q2QYH7	7.4e-84	35.44	Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ...	[more]
B6SSW8	7.0e-82	33.73	Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1	[more]
Q5KQH7	7.7e-81	31.67	Cytochrome P450 714D1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714D1 PE=1 ...	[more]

BLAST of MC10g0127 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141037.1	0.0	100.00	cytokinin hydroxylase [Momordica charantia]	[more]
KAG6574181.1	1.74e-292	78.72	Cytochrome P450 714C2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022945852.1	7.06e-292	78.53	cytochrome P450 714C2-like [Cucurbita moschata]	[more]
XP_038877847.1	5.92e-290	78.49	cytokinin hydroxylase-like [Benincasa hispida]	[more]
XP_022968014.1	1.35e-289	78.53	cytochrome P450 714C2-like [Cucurbita maxima]	[more]

BLAST of MC10g0127 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CIQ8	0.0	100.00	cytokinin hydroxylase OS=Momordica charantia OX=3673 GN=LOC111011538 PE=3 SV=1	[more]
A0A6J1G224	3.42e-292	78.53	cytochrome P450 714C2-like OS=Cucurbita moschata OX=3662 GN=LOC111449972 PE=3 SV...	[more]
A0A6J1HYD5	6.56e-290	78.53	cytochrome P450 714C2-like OS=Cucurbita maxima OX=3661 GN=LOC111467387 PE=3 SV=1	[more]
A0A1S3AXX2	1.00e-282	76.49	cytokinin hydroxylase OS=Cucumis melo OX=3656 GN=LOC103484084 PE=3 SV=1	[more]
A0A5D3DI76	1.03e-260	76.57	Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold38...	[more]

BLAST of MC10g0127 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52400.1	7.9e-174	58.11	cytochrome P450, family 715, subfamily A, polypeptide 1	[more]
AT5G38450.1	6.7e-88	35.40	cytochrome P450, family 735, subfamily A, polypeptide 1	[more]
AT1G67110.1	3.7e-86	35.54	cytochrome P450, family 735, subfamily A, polypeptide 2	[more]
AT5G24900.1	9.3e-82	33.27	cytochrome P450, family 714, subfamily A, polypeptide 2	[more]
AT2G26710.1	1.1e-79	32.34	Cytochrome P450 superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 88..107 score: 24.75 coord: 464..487 score: 30.56 coord: 314..331 score: 28.15 coord: 454..464 score: 51.95 coord: 334..360 score: 35.84
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 377..388 score: 41.1 coord: 325..342 score: 37.37 coord: 464..475 score: 36.49 coord: 455..464 score: 57.77
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 80..492 e-value: 2.3E-84 score: 283.8
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 16..517 e-value: 2.3E-109 score: 368.1
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 73..515
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 2..512
None	No IPR available	PANTHER	PTHR24282:SF149	CYTOCHROME P450 FAMILY 72 PROTEIN	coord: 2..512
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 457..466

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC10g0127.1	MC10g0127.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen