MC10g0085 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0085
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptioncation/H(+) antiporter 3-like
LocationMC10: 532148 .. 535507 (-)
RNA-Seq ExpressionMC10g0085
SyntenyMC10g0085
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTCTTTTTGTTCAACCTTGGCGGGTTAGCTCTGACGGTATTTGGGGAAAGTTCTCTCGCCCTCAATGGTGGCTCAATAATTCATTGCCTCTCTTGGAGCTGCAGCTCTTCTTGTTCTTTCTCCTCATGTCAATTATTCATCTTCTCCTCAAGCCTTTTGGGGTCTCCAAGATTTCCTCTCAAATTATTGTACGTACACACCAAATTCCAATCCATGTTAGTTCTGTTTCTGTATTGTTTGGTTCTTCCTTAATTCCTTTGCCTGCCCCTCATGCATGTTTTAGAGATAGCCACATTCCTTTCTGGGTTTATATCAATTGTGTTTCTTCTCACTATGGTGAAAAAATTGAATCAGATTGAGTTACATTGTCAAGTAATGCATGAATGCACACGAACATGAGTTCAGCTCAACGGTTAGAACATATTAGTAAATCTTTCTTACGAAAGTTTGAATTCTCACCTTATAATCATAATGGAAAAAGAAACATGAATACAAATATAAATAAGTTCATGTATAATTTATCTATTGAAAAACATGTTTAAGGTCCAATTTATATATGTAATCATCAATTTTGTGAAAGAAACTAATTTTTTGAGGTTGTTTAGAAATGGGTTATTTCTTGTAATTTTTGTCAACTCAATCATCATCATAAACTTTGTAATCACATACACTTTGTAGACAAAAAGAGGCAAGTTTTAATTTTCCTGTGATATAACTATTGATGTGAGTGAATACGATGAAAAAATACTAACTTTTGATATTTTGGCAGACTGGTCTGGTATTTGGTTGTTCATGGGGCCAATTGAACAACAAAAAATTGGAATTTTTTAGGATTAACAGTGAAGAGATCATAGGGCTGTTAGCGTGCTTCGGCTACATGCTATTTTTGTTCATAACAGCAGTGAAAATGGACGTGAGAAGGACCATGAAAACAGGAAGAAAGGCCTGGATAATCGGAATCCCTTCAGTGTTCGTGCCTGTAATTTGTGGTCTTTTCGTTGGATCTTATTTCCAAGATGGCTTAAGTTCCGCAGAGAAAAATACAGTCCCTCTAATGGTTAGCATCCATAGTTTTATCTCGTTCCCAGTGATTGCTTCTCTGCTGAGCGAGCTGAAGTTGGTGAGCACAGAACTTGGCCGTTTGGGGATGTCCTCAGCATTGATTGCTGACCTATTCAGCCAATTTGTTATGGGGACTGCAAACCAAATCAGAATCTCCAGAACCAGCATTACACTCGGCTATTTTAGCAAGGCAGGAATTATGGTTCAAATTTGCCTCATACTTTTTGTGTTGAGGCCATCAGTGCTTTGGCTTTTGAAACAGACGCCTGAGGGGAAGCCAGTGAGCCGTGGCACTACTCACGGCATTTTTCTGTTTGTTCTGATTTCTGCTGTTGCTTCTGCTCTTTTGGGGCAGCCAGCAATTTTTGGGCCTTATCTTTTGGGGCTGGCCATCCCTGATGGAGCTACACAATTGGGATTCTCACTGGTGGAGAAGCTGGAGTGCTTTGTTTCGGATTTCTTTCTGCCATTGTTTGTCATAACATGTTCTCTACAGGTGGATATTTCCAAGATATTCATTGTCCTTGGAGCTGCTGATGCAAGAGTTAACATTGTTCTTTCACTTGTCACTTATGGAGTCAAACTGGTGTGTTGCTTCCTTTGTTCTTTGTGTTGCAAGTTGCCCTTGAGAGATTCCTTCGTGCTGTCCCTCATTCTCAGCTGCAAAGGTGTTTTTGAATTGGCCTTCTGCTCAATCATCACTCAATATGAGGTAAGCAGGTTTCGAGATGAATTCTTGAATAATATAGTTAACATGTCGCACAATTGTTAAAATTGATCATAATTAACCGCGTAGACTTATTAAGAACTTTTTAAAGTACAAAGAGTAAATAGAGATGCAACTATGAAAATTTAGTCATCTATTAAGAGTTTTTAAAGTGCACAAACTAGCAGATACAACTCTGAAAAGTTCAAACAACCGAATATATGATTTAACCCTTTTTTCCTTGTCAGGTTAAAATCACTTCTCATTTTTTTCTTTGATCCCACTTGCAGGTTATTAACCGAGGGATTCTGGCATGGTTCATAGTGTTCTTATTAATAGTTGCAACCTTTGTGCCACTGACAGTGAAGTGGCTCACGGACCCCAAAAGGACACATGATGGCTATCAGAACAGAAACATCATGCATTTAAGCCCCAACTCAGAATTGCGAGTCCTTCCCTGCATTCACAAAAACGAACACATCTATGGCCTCATCCATCTCCTCAATCTCTCATGTCCAACGCCGCAAAATCCGCTTGCTGTTTACGCCCTTCACCTCATTGAGCTTGTCGGCCGCACCACCCCCATCTTCATTTCCCATAGACAGGAAAACAATGCCATTTCCGACCAAACCTACTCAGAAAATGTCACTCTCTCCTTTGACCACTTTGAGAAGGACAACTCTGCAGGCTCTGTCTATGCTGAATGCTTCACAGTAATCACACCCCACAAGTTCATGACCAATGACATCTGCAAATTGGCGCTTGACAAGGTCACGTCTCTTATAATTCTGCCCTTTCACAGGACATGGACGGCGGATGGCCTTATAGACCAAGAGGACAACATGTTGAGGAATTTGAACTGCAGTGTGCTCCAGAAGGCGGATTGTTCAGTGGCAATTTTTGCTGACAAAGGGCATTTAGGGAGCGTTGCTTCTATGGCTTCTTCTGGCACACAATTTAGATATTCAGTTTGCTTGATATATTTGGGAGGGAGTGATGACAGGGAGGCAATTTCTTTTGCCAAACGCATGGCAAAGGAGCCCACAATTGAGCTCACTGTGCTGAAACTTGGGTCTCCTATGGAAGATGGGTCGAGTAGCAAATGGGAGAACATGCTTGATTCTGAGGTTCTAAAGGATTTCAAAATGAACTGTTTGGGGAATGGGGGAGTGAGGTATTTTGAGGAAGTGTGTGATGATGGGCCTCAAACAGCACTGACACTTAGAAAAATTGTGAATGAGTTTGATCTTATGATAGTAGGGAGAAGAGATGGGTTGGAATCTCCTCAGACTGCTGGGCTTAGTGAGTGGAATGAGTTTCCAGAGCTTGGAGTTCTTGGAGATTTGATTGCTTCATTAGATAACAACACAAGAACTTCAGTGTTGGTAATACAACAGCAGAAGTAGTTAATTCACTAGACATCAAGATGTGTATGTAGTTCAATTATCTTTTTGCAAGAATACATACAGATAGTTTAATTTCCTTGTCAGTGGACAAGGCACAAAGTAAAAGAACAATACTCTTGTTTATGATCAACATTTTTGCAGTTTGTCTTAACTTTTAGTGAACATCTTATAAATGAATTAAGGGA

mRNA sequence

TGTCTTTTTGTTCAACCTTGGCGGGTTAGCTCTGACGGTATTTGGGGAAAGTTCTCTCGCCCTCAATGGTGGCTCAATAATTCATTGCCTCTCTTGGAGCTGCAGCTCTTCTTGTTCTTTCTCCTCATGTCAATTATTCATCTTCTCCTCAAGCCTTTTGGGGTCTCCAAGATTTCCTCTCAAATTATTACTGGTCTGGTATTTGGTTGTTCATGGGGCCAATTGAACAACAAAAAATTGGAATTTTTTAGGATTAACAGTGAAGAGATCATAGGGCTGTTAGCGTGCTTCGGCTACATGCTATTTTTGTTCATAACAGCAGTGAAAATGGACGTGAGAAGGACCATGAAAACAGGAAGAAAGGCCTGGATAATCGGAATCCCTTCAGTGTTCGTGCCTGTAATTTGTGGTCTTTTCGTTGGATCTTATTTCCAAGATGGCTTAAGTTCCGCAGAGAAAAATACAGTCCCTCTAATGGTTAGCATCCATAGTTTTATCTCGTTCCCAGTGATTGCTTCTCTGCTGAGCGAGCTGAAGTTGGTGAGCACAGAACTTGGCCGTTTGGGGATGTCCTCAGCATTGATTGCTGACCTATTCAGCCAATTTGTTATGGGGACTGCAAACCAAATCAGAATCTCCAGAACCAGCATTACACTCGGCTATTTTAGCAAGGCAGGAATTATGGTTCAAATTTGCCTCATACTTTTTGTGTTGAGGCCATCAGTGCTTTGGCTTTTGAAACAGACGCCTGAGGGGAAGCCAGTGAGCCGTGGCACTACTCACGGCATTTTTCTGTTTGTTCTGATTTCTGCTGTTGCTTCTGCTCTTTTGGGGCAGCCAGCAATTTTTGGGCCTTATCTTTTGGGGCTGGCCATCCCTGATGGAGCTACACAATTGGGATTCTCACTGGTGGAGAAGCTGGAGTGCTTTGTTTCGGATTTCTTTCTGCCATTGTTTGTCATAACATGTTCTCTACAGGTGGATATTTCCAAGATATTCATTGTCCTTGGAGCTGCTGATGCAAGAGTTAACATTGTTCTTTCACTTGTCACTTATGGAGTCAAACTGGTGTGTTGCTTCCTTTGTTCTTTGTGTTGCAAGTTGCCCTTGAGAGATTCCTTCGTGCTGTCCCTCATTCTCAGCTGCAAAGGTGTTTTTGAATTGGCCTTCTGCTCAATCATCACTCAATATGAGGTTATTAACCGAGGGATTCTGGCATGGTTCATAGTGTTCTTATTAATAGTTGCAACCTTTGTGCCACTGACAGTGAAGTGGCTCACGGACCCCAAAAGGACACATGATGGCTATCAGAACAGAAACATCATGCATTTAAGCCCCAACTCAGAATTGCGAGTCCTTCCCTGCATTCACAAAAACGAACACATCTATGGCCTCATCCATCTCCTCAATCTCTCATGTCCAACGCCGCAAAATCCGCTTGCTGTTTACGCCCTTCACCTCATTGAGCTTGTCGGCCGCACCACCCCCATCTTCATTTCCCATAGACAGGAAAACAATGCCATTTCCGACCAAACCTACTCAGAAAATGTCACTCTCTCCTTTGACCACTTTGAGAAGGACAACTCTGCAGGCTCTGTCTATGCTGAATGCTTCACAGTAATCACACCCCACAAGTTCATGACCAATGACATCTGCAAATTGGCGCTTGACAAGGTCACGTCTCTTATAATTCTGCCCTTTCACAGGACATGGACGGCGGATGGCCTTATAGACCAAGAGGACAACATGTTGAGGAATTTGAACTGCAGTGTGCTCCAGAAGGCGGATTGTTCAGTGGCAATTTTTGCTGACAAAGGGCATTTAGGGAGCGTTGCTTCTATGGCTTCTTCTGGCACACAATTTAGATATTCAGTTTGCTTGATATATTTGGGAGGGAGTGATGACAGGGAGGCAATTTCTTTTGCCAAACGCATGGCAAAGGAGCCCACAATTGAGCTCACTGTGCTGAAACTTGGGTCTCCTATGGAAGATGGGTCGAGTAGCAAATGGGAGAACATGCTTGATTCTGAGGTTCTAAAGGATTTCAAAATGAACTGTTTGGGGAATGGGGGAGTGAGGTATTTTGAGGAAGTGTGTGATGATGGGCCTCAAACAGCACTGACACTTAGAAAAATTGTGAATGAGTTTGATCTTATGATAGTAGGGAGAAGAGATGGGTTGGAATCTCCTCAGACTGCTGGGCTTAGTGAGTGGAATGAGTTTCCAGAGCTTGGAGTTCTTGGAGATTTGATTGCTTCATTAGATAACAACACAAGAACTTCAGTGTTGGTAATACAACAGCAGAAGTAGTTAATTCACTAGACATCAAGATGTGTATGTAGTTCAATTATCTTTTTGCAAGAATACATACAGATAGTTTAATTTCCTTGTCAGTGGACAAGGCACAAAGTAAAAGAACAATACTCTTGTTTATGATCAACATTTTTGCAGTTTGTCTTAACTTTTAGTGAACATCTTATAAATGAATTAAGGGA

Coding sequence (CDS)

TGTCTTTTTGTTCAACCTTGGCGGGTTAGCTCTGACGGTATTTGGGGAAAGTTCTCTCGCCCTCAATGGTGGCTCAATAATTCATTGCCTCTCTTGGAGCTGCAGCTCTTCTTGTTCTTTCTCCTCATGTCAATTATTCATCTTCTCCTCAAGCCTTTTGGGGTCTCCAAGATTTCCTCTCAAATTATTACTGGTCTGGTATTTGGTTGTTCATGGGGCCAATTGAACAACAAAAAATTGGAATTTTTTAGGATTAACAGTGAAGAGATCATAGGGCTGTTAGCGTGCTTCGGCTACATGCTATTTTTGTTCATAACAGCAGTGAAAATGGACGTGAGAAGGACCATGAAAACAGGAAGAAAGGCCTGGATAATCGGAATCCCTTCAGTGTTCGTGCCTGTAATTTGTGGTCTTTTCGTTGGATCTTATTTCCAAGATGGCTTAAGTTCCGCAGAGAAAAATACAGTCCCTCTAATGGTTAGCATCCATAGTTTTATCTCGTTCCCAGTGATTGCTTCTCTGCTGAGCGAGCTGAAGTTGGTGAGCACAGAACTTGGCCGTTTGGGGATGTCCTCAGCATTGATTGCTGACCTATTCAGCCAATTTGTTATGGGGACTGCAAACCAAATCAGAATCTCCAGAACCAGCATTACACTCGGCTATTTTAGCAAGGCAGGAATTATGGTTCAAATTTGCCTCATACTTTTTGTGTTGAGGCCATCAGTGCTTTGGCTTTTGAAACAGACGCCTGAGGGGAAGCCAGTGAGCCGTGGCACTACTCACGGCATTTTTCTGTTTGTTCTGATTTCTGCTGTTGCTTCTGCTCTTTTGGGGCAGCCAGCAATTTTTGGGCCTTATCTTTTGGGGCTGGCCATCCCTGATGGAGCTACACAATTGGGATTCTCACTGGTGGAGAAGCTGGAGTGCTTTGTTTCGGATTTCTTTCTGCCATTGTTTGTCATAACATGTTCTCTACAGGTGGATATTTCCAAGATATTCATTGTCCTTGGAGCTGCTGATGCAAGAGTTAACATTGTTCTTTCACTTGTCACTTATGGAGTCAAACTGGTGTGTTGCTTCCTTTGTTCTTTGTGTTGCAAGTTGCCCTTGAGAGATTCCTTCGTGCTGTCCCTCATTCTCAGCTGCAAAGGTGTTTTTGAATTGGCCTTCTGCTCAATCATCACTCAATATGAGGTTATTAACCGAGGGATTCTGGCATGGTTCATAGTGTTCTTATTAATAGTTGCAACCTTTGTGCCACTGACAGTGAAGTGGCTCACGGACCCCAAAAGGACACATGATGGCTATCAGAACAGAAACATCATGCATTTAAGCCCCAACTCAGAATTGCGAGTCCTTCCCTGCATTCACAAAAACGAACACATCTATGGCCTCATCCATCTCCTCAATCTCTCATGTCCAACGCCGCAAAATCCGCTTGCTGTTTACGCCCTTCACCTCATTGAGCTTGTCGGCCGCACCACCCCCATCTTCATTTCCCATAGACAGGAAAACAATGCCATTTCCGACCAAACCTACTCAGAAAATGTCACTCTCTCCTTTGACCACTTTGAGAAGGACAACTCTGCAGGCTCTGTCTATGCTGAATGCTTCACAGTAATCACACCCCACAAGTTCATGACCAATGACATCTGCAAATTGGCGCTTGACAAGGTCACGTCTCTTATAATTCTGCCCTTTCACAGGACATGGACGGCGGATGGCCTTATAGACCAAGAGGACAACATGTTGAGGAATTTGAACTGCAGTGTGCTCCAGAAGGCGGATTGTTCAGTGGCAATTTTTGCTGACAAAGGGCATTTAGGGAGCGTTGCTTCTATGGCTTCTTCTGGCACACAATTTAGATATTCAGTTTGCTTGATATATTTGGGAGGGAGTGATGACAGGGAGGCAATTTCTTTTGCCAAACGCATGGCAAAGGAGCCCACAATTGAGCTCACTGTGCTGAAACTTGGGTCTCCTATGGAAGATGGGTCGAGTAGCAAATGGGAGAACATGCTTGATTCTGAGGTTCTAAAGGATTTCAAAATGAACTGTTTGGGGAATGGGGGAGTGAGGTATTTTGAGGAAGTGTGTGATGATGGGCCTCAAACAGCACTGACACTTAGAAAAATTGTGAATGAGTTTGATCTTATGATAGTAGGGAGAAGAGATGGGTTGGAATCTCCTCAGACTGCTGGGCTTAGTGAGTGGAATGAGTTTCCAGAGCTTGGAGTTCTTGGAGATTTGATTGCTTCATTAGATAACAACACAAGAACTTCAGTGTTGGTAATACAACAGCAGAAGTAG

Protein sequence

CLFVQPWRVSSDGIWGKFSRPQWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSKISSQIITGLVFGCSWGQLNNKKLEFFRINSEEIIGLLACFGYMLFLFITAVKMDVRRTMKTGRKAWIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPLMVSIHSFISFPVIASLLSELKLVSTELGRLGMSSALIADLFSQFVMGTANQIRISRTSITLGYFSKAGIMVQICLILFVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLGLAIPDGATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAADARVNIVLSLVTYGVKLVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFIVFLLIVATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLLNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNSAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQKADCSVAIFADKGHLGSVASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK
Homology
BLAST of MC10g0085 vs. ExPASy Swiss-Prot
Match: Q9FFB8 (Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 4.3e-136
Identity = 299/804 (37.19%), Postives = 471/804 (58.58%), Query Frame = 0

Query: 4   VQPWRVSSDGIW--GKFSRP----QWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSK 63
           V P   SS+G+W   KFS P     +W N + P L++   +   L   +H  L+  G+ +
Sbjct: 25  VLPINPSSNGVWPQQKFSDPNINVHFW-NYAFPHLQMIFLIISFLWQFLHFFLRRLGMIR 84

Query: 64  ISSQIITGLVFGCSWGQLNNKKLEFFRIN--SEEIIGLLACFGYMLFLFITAVKMDVRRT 123
            +S ++TG++   S+ + N+    FF      E +  L A   YM+F F+  VKMD    
Sbjct: 85  FTSHMLTGVLLSKSFLKENSAARRFFSTEDYKEIVFSLTAACSYMMFWFLMGVKMDTGLI 144

Query: 124 MKTGRKAWIIGIPSVFV-PVICG-LFVGSYFQDGLSSAEK--NTVPLMV--SIHSFISFP 183
             TGRKA  IG+ SV +  ++C  +F G+    G  +++   N++  +V  SI    SFP
Sbjct: 145 RTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSDHTLNSLEYVVIYSIQCLSSFP 204

Query: 184 VIASLLSELKLVSTELGRLGMSSALIADLFSQFVMGTA----NQIRISRT---SITLG-- 243
           V+ +LL EL+L ++ELGRL +SSA+I+D FS  ++ +      +++  +T   S+ +G  
Sbjct: 205 VVGNLLFELRLQNSELGRLAISSAVISD-FSTSILASVLIFMKELKDEQTRLGSVFIGDV 264

Query: 244 -----YFSKAGIMVQ-ICLILFVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVAS 303
                   +AGI+V  +C+ ++V RP + +++KQTP G+PV       I + V  SA+ +
Sbjct: 265 IAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQTPSGRPVKAIYLSTIIVMVSGSAILA 324

Query: 304 ALLGQPAIFGPYLLGLAIPDGATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFI 363
               Q    GP++LGLA+P G   LG ++++K E  +   FLP F+ + S ++DIS +F 
Sbjct: 325 NWCKQSIFMGPFILGLAVPHG-PPLGSAIIQKYESAIFGTFLPFFIASSSTEIDISALF- 384

Query: 364 VLGAADARVNIVLSLVTYGVKLVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSII 423
             G       I++ + ++ VK +   + +L   +P+ D F LSLI+S KG+FEL   ++ 
Sbjct: 385 --GWEGLNGIILIMVTSFVVKFIFTTVPALFYGMPMEDCFALSLIMSFKGIFELGAYALA 444

Query: 424 TQYEVINRGILAWFIVFLLIVATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLP 483
            Q   +         +++ + +  +P  +++L DP R + GY+ RN+ HL PNSELR+L 
Sbjct: 445 YQRGSVRPETFTVACLYITLNSAIIPPILRYLYDPSRMYAGYEKRNMQHLKPNSELRILS 504

Query: 484 CIHKNEHIYGLIHLLNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTYS 543
           CI++ + I  +I+LL   CP+ ++P+A Y LHL+ELVG+  PIFISH+ +     + +YS
Sbjct: 505 CIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQANPIFISHKLQTRRTEETSYS 564

Query: 544 ENVTLSFDHFEKDNSAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTAD 603
            NV +SF+ F KD   GSV+   +T ++    M  DIC LAL+  TSLI+LPFH+TW+AD
Sbjct: 565 NNVLVSFEKFRKD-FYGSVFVSTYTALSMPDTMHGDICMLALNNTTSLILLPFHQTWSAD 624

Query: 604 G-LIDQEDNMLRNLNCSVLQKADCSVAIFADKGHLG----SVASMASSGT---QFRYSVC 663
           G  +   +NM+RNLN SVL  A CSV +F  +   G    S      +GT      Y++C
Sbjct: 625 GSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKNISSGRKTINGTVPNLSSYNIC 684

Query: 664 LIYLGGSDDREAISFAKRMAKEPTIELTVLKLGSPMEDG-SSSKWENMLDSEVLKDFKMN 723
           +I+LGG DDREA++ A RMA++P I +T+++L +  E    ++ W+ MLD E+L+D K N
Sbjct: 685 MIFLGGKDDREAVTLATRMARDPRINITIVRLITTDEKARENTVWDKMLDDELLRDVKSN 744

Query: 724 CLGNGGVRYFEEVCDDGPQTALTLRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELG 770
            L +  + Y E+  +D  +T+  LR +V++FD+ IVGR +G  S  T GL EW+EF ELG
Sbjct: 745 TLVD--IFYSEKAIEDAAETSSLLRSMVSDFDMFIVGRGNGRTSVFTEGLEEWSEFKELG 804

BLAST of MC10g0085 vs. ExPASy Swiss-Prot
Match: Q9FYC1 (Cation/H(+) antiporter 4 OS=Arabidopsis thaliana OX=3702 GN=CHX4 PE=2 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 1.8e-134
Identity = 292/797 (36.64%), Postives = 448/797 (56.21%), Query Frame = 0

Query: 4   VQPWRVSSDGIWGKFSRP------QWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSK 63
           + P   SS G+W     P      ++W N   P +++   +  +L    H  L+  G+ +
Sbjct: 24  ILPINPSSSGLWPSPKLPDPQANIEFW-NYMFPHVQIIFLIVTILWQFFHFFLRRLGMIR 83

Query: 64  ISSQIITGLVFGCSWGQLNNKKLEFFRIN--SEEIIGLLACFGYMLFLFITAVKMDVRRT 123
            +S ++TG++   S+ + N    +F       E + GL+    YM+F F+  VKMD+   
Sbjct: 84  FTSHMLTGILLSKSFLKENTPARKFLSTEDYKETLFGLVGACSYMMFWFLMGVKMDLSLI 143

Query: 124 MKTGRKAWIIGIPSVFVPV-ICGLFVGSYFQD-GLSSAEK----NTVPLMVSIHSFISFP 183
             TGRKA  IG+ SV + + +C L      +D G    E       +  +  I    SFP
Sbjct: 144 RSTGRKAVAIGLSSVLLSITVCALIFFLILRDVGTKKGEPVMSFFEIIFIYLIQCLSSFP 203

Query: 184 VIASLLSELKLVSTELGRLGMSSALIADLFSQF---VMGTANQIRISRT---SITLG--- 243
           VI +LL EL+L ++ELGRL MSSA+I+D  +     V+    +++  ++   S+ +G   
Sbjct: 204 VIGNLLFELRLQNSELGRLAMSSAVISDFSTSILSAVLVFLKELKDDKSRLGSVFIGDVI 263

Query: 244 ----YFSKAG-IMVQICLILFVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASA 303
                  +AG +++ +C  +++ RP + +++K+TP G+PV +   + I + V  SA+ + 
Sbjct: 264 VGNRPMKRAGTVVLFVCFAIYIFRPLMFFIIKRTPSGRPVKKFYIYAIIILVFGSAILAD 323

Query: 304 LLGQPAIFGPYLLGLAIPDGATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIV 363
              Q    GP++LGLA+P G   LG ++++K E  V   FLP FV T + ++D S   I+
Sbjct: 324 WCKQSIFIGPFILGLAVPHG-PPLGSAILQKFESVVFGTFLPFFVATSAEEIDTS---IL 383

Query: 364 LGAADARVNIVLSLVTYGVKLVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIIT 423
               D +  ++L  V++ VK     L +    +P +D   LSLI+S KG+FE        
Sbjct: 384 QSWIDLKSIVILVSVSFIVKFALTTLPAFLYGMPAKDCIALSLIMSFKGIFEFGAYGYAY 443

Query: 424 QYEVINRGILAWFIVFLLIVATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPC 483
           Q   I         +++L+ +  +P  +K + DP R + GY+ RN++H+ PNSELR+L C
Sbjct: 444 QRGTIRPVTFTVLSLYILLNSAVIPPLLKRIYDPSRMYAGYEKRNMLHMKPNSELRILSC 503

Query: 484 IHKNEHIYGLIHLLNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTY-S 543
           I+K + I  +I+LL  +CP+ +NP+A Y LHL+ELVG+  P+ ISHR +     + +Y S
Sbjct: 504 IYKTDDIRPMINLLEATCPSRENPVATYVLHLMELVGQANPVLISHRLQTRKSENMSYNS 563

Query: 544 ENVTLSFDHFEKDNSAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTAD 603
           ENV +SF+ F  D   GSV+   +T ++  K M  DIC LAL+  TSLIILPFH+TW+AD
Sbjct: 564 ENVVVSFEQFHND-FFGSVFVSTYTALSVPKMMHGDICMLALNNTTSLIILPFHQTWSAD 623

Query: 604 G-LIDQEDNMLRNLNCSVLQKADCSVAIFADKGHLGSVASMASSGTQFRYSVCLIYLGGS 663
           G  I  +  M+R LN SVL  + CSV IF  +   G      ++     Y VC+++LGG 
Sbjct: 624 GSAIVSDSLMIRQLNKSVLDLSPCSVGIFVYRSSNGRRTIKETAANFSSYQVCMLFLGGK 683

Query: 664 DDREAISFAKRMAKEPTIELTVLKL-GSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGV 723
           DDREA+S AKRMA++  I +TV+ L  S      ++ W+ MLD E+L+D K N L    +
Sbjct: 684 DDREALSLAKRMARDSRITITVVSLISSEQRANQATDWDRMLDLELLRDVKSNVLAGADI 743

Query: 724 RYFEEVCDDGPQTALTLRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIA 770
            + EEV +D  QT+  L+ I NE+DL IVGR  G +S  T GL EW+EF ELG++GDL+ 
Sbjct: 744 VFSEEVVNDANQTSQLLKSIANEYDLFIVGREKGRKSVFTEGLEEWSEFEELGIIGDLLT 803

BLAST of MC10g0085 vs. ExPASy Swiss-Prot
Match: Q9FYC0 (Cation/H(+) antiporter 12 OS=Arabidopsis thaliana OX=3702 GN=CHX12 PE=2 SV=2)

HSP 1 Score: 438.7 bits (1127), Expect = 1.3e-121
Identity = 277/781 (35.47%), Postives = 424/781 (54.29%), Query Frame = 0

Query: 7   WRVSSDGIWGKFSRPQWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSKISSQIITGL 66
           + +SS G W     P      SLPL+E Q+ L F+ + IIH  LK FG+S I S ++ GL
Sbjct: 15  FNISSFGFWENLKSPDVIFGYSLPLMEFQILLIFVFIIIIHSFLKSFGISPIPSYMLAGL 74

Query: 67  VFG---CSWGQLNNKKLEFFRI--NSEEIIGLLACFGYMLFLFITAVKMDVRRTMKTGRK 126
           + G    +  +++++KL +      +  + GL  C   ML  F+T VK+  R     G  
Sbjct: 75  ILGPQLFNLREVSSRKLSWDPALDGNGPLRGLSVCGNIMLAFFMT-VKISRRLAFNNGWL 134

Query: 127 AWIIGIPSVFVPVICGLFVGSYFQDGLSS--AEKNTV----PLMVSIHSFISFPVIASLL 186
             +IG  S  VP + G  V +   D +       N V     +++S  S I  P +   L
Sbjct: 135 PIVIGTLSFIVPFLGGFCVRNLHTDNIDPYYMSPNKVLAERIVVISSQSSILLPTVVHFL 194

Query: 187 SELKLVSTELGRLGMSSALIADLFSQ------FVMGTANQIRISRTSITLGYFSKAGIMV 246
           SELK++++ELGRL +S++LI D+F+       +++GT   I     S    Y     +++
Sbjct: 195 SELKILNSELGRLVLSASLINDIFASTVSIFAYLVGTYKNI-----SPMTAYRDLIAVII 254

Query: 247 QICLILFVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLG 306
            I +   VLRP V W++++TPEGKPV+    H + L V+ SA  S+      + GP+LLG
Sbjct: 255 LILVAFCVLRPVVEWIVERTPEGKPVADVYVHAVVLSVIASAAYSSFFNMKYLLGPFLLG 314

Query: 307 LAIPDGATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAADARVNIVLSL 366
           + IP+G   +G +L  K E    +  +P+ +   +++ D+ K  IV    D   NI L  
Sbjct: 315 IIIPEG-PPIGSALEAKYEALTMNVLIPISITFSTMRCDVMK--IVYQYDDIWYNIFLMT 374

Query: 367 VTYGVKLVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFI 426
            T  +K+    +  L CK+P +++   SL+L  K   E+           I++    + I
Sbjct: 375 FTGFLKMATGMVPCLYCKIPFKEAIAASLLLCSKSFSEIFLYESTYDDSYISQATYTFLI 434

Query: 427 VFLLIVATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLL 486
              LI +  +P  +  L DPKR + GYQ +NIM+L P+S+LR+L CIH+ E+I   I  L
Sbjct: 435 TCALINSGIIPTALAGLYDPKRKYVGYQKKNIMNLKPDSDLRILTCIHRPENISAAISFL 494

Query: 487 NLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNS 546
                T    + V  LHL++LVG+T P+ ISH ++ N +   +Y     L+F   E    
Sbjct: 495 QFLPST----IVVTVLHLVKLVGKTVPVLISHNKQINRVVTNSYIHTANLAFSQLE---- 554

Query: 547 AGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNC 606
             SV    FT IT    M ++ICK+AL++ TS+II+P  R WT DG  + ED  +R LN 
Sbjct: 555 --SVTMTMFTAITHENLMHDEICKVALEQATSIIIVPSGRKWTVDGAFESEDEAIRRLNE 614

Query: 607 SVLQKADCSVAIFADKGHLGSVASMASSGT-QFRYSVCLIYLGGSDDREAISFAKRMAKE 666
           S+L+ A CS+ I  D+G L      +  GT +F   V +I++GG DDREA+S  K+M + 
Sbjct: 615 SLLKSASCSIGILVDRGQL------SLKGTRKFNIDVGVIFIGGKDDREALSLVKKMKQN 674

Query: 667 PTIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALT 726
           P +++TV++L S  E   S+ W+ +LD EVL+D K +      + Y E +   GP+ A T
Sbjct: 675 PRVKITVIRLISDRET-ESTNWDYILDHEVLEDLK-DTEATNSIAYTERIVTGGPEVATT 734

Query: 727 LRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQ 770
           +R +  ++DLM+VGR  G+ SP   GL EW E PELGV+GDL+AS + ++R SVLV+QQQ
Sbjct: 735 VRSLSEDYDLMVVGRDHGMASPDFDGLMEWVELPELGVIGDLLASRELDSRVSVLVVQQQ 768

BLAST of MC10g0085 vs. ExPASy Swiss-Prot
Match: Q9FYB9 (Cation/H(+) antiporter 11 OS=Arabidopsis thaliana OX=3702 GN=CHX11 PE=2 SV=2)

HSP 1 Score: 425.2 bits (1092), Expect = 1.5e-117
Identity = 265/775 (34.19%), Postives = 414/775 (53.42%), Query Frame = 0

Query: 7   WRVSSDGIWGKFSRPQWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSKISSQIITGL 66
           + +SS G W     P      SLPLLE+Q+ L F  + + H+ L+  GVS+I S +I GL
Sbjct: 15  FNISSQGFWENLKSPDVVFGYSLPLLEIQIILIFFCIVMSHMFLRCIGVSQIVSYMIAGL 74

Query: 67  VFGCSWGQLNNK---KLEFF-RINSEEIIGLLACFGYMLFLFITAVKMDVRRTMKTGRKA 126
           + G     +  K   KL     ++    +  ++ FG ++F F+  V+   R    +G+  
Sbjct: 75  ILGPQLFDILEKSSGKLSADPALDGTAALRCISVFGRLMFTFLMTVRTSRRVAFHSGKLP 134

Query: 127 WIIGIPSVFVPVICGLFVGSYFQDGLS----SAEKNTV--PLMVSIHSFISFPVIASLLS 186
            +IGI S F P+    F+ + F D +     S +K      ++V   S I  P    +L 
Sbjct: 135 VVIGIVSFFAPLFSLSFL-NLFTDNIDPHYMSLDKALAERTVIVITQSQILLPSTTYILL 194

Query: 187 ELKLVSTELGRLGMSSALIADLFSQFVMGTA-NQIRISRTSITLGYFSKAGIMVQICLIL 246
           ELK++++ELGRL +S++ I D+   F M  A  Q      S  + Y     +++   ++ 
Sbjct: 195 ELKIINSELGRLALSASAINDMLGIFAMIVATTQATYIHVSHAIAYRDLVAVIIFFLIVF 254

Query: 247 FVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLGLAIPDG 306
           FV +P V W++ +TPE KPV     H + L    SA          + GP ++G+ IP+G
Sbjct: 255 FVFKPMVQWIIDRTPEDKPVEDIYIHAVILTAFASAAYFVFFNMKYVLGPLIIGIIIPEG 314

Query: 307 ATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAADARVNIVLSLVTYGVK 366
              LG +L  K E    + FLP+ +   +++ D   + I+    D   NI L+L+   +K
Sbjct: 315 -PPLGSALEAKFERLTMNVFLPISITFSAMRCD--GLRILSQFTDIYFNIFLTLLILVIK 374

Query: 367 LVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFIVFLLIV 426
           LV C    L  KLP  +S  +SLILS K   E      + + + I++   A+ I++ L+ 
Sbjct: 375 LVACLTLCLYYKLPRSESLAVSLILSYKSFVEFVLYEAVLEEKFISQATYAFLILYSLLS 434

Query: 427 ATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLLNL-SCP 486
           A  VP+ V+ + DPKR +  YQ R+I+HL  NS LR+L C+HK E++   I  L L S P
Sbjct: 435 AGIVPMVVRSMYDPKRKYVNYQKRDILHLEANSGLRILTCLHKPENVSETIAFLQLFSSP 494

Query: 487 TPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNSAGSVY 546
               P+AV  LHL++LVG+  PI +SH ++   +   +Y     L+F  F ++ S  SV 
Sbjct: 495 IHDFPIAVTVLHLVKLVGQINPIIVSHDKKLKRLHKNSYIHTANLAFRQFMQE-SLESVT 554

Query: 547 AECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQK 606
              FT  +    M  DIC LALD+ TS+I++P  R WT DG+ + +D   R LN S+L +
Sbjct: 555 VTTFTAFSHENLMHEDICTLALDRTTSMIVVPSGRKWTVDGMFESDDLAARQLNQSLLDR 614

Query: 607 ADCSVAIFADKGHLGSVASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELT 666
           A CS+ I  D+G   S  S  +S  ++   V ++++GG DDREA+S  KRM   P + +T
Sbjct: 615 APCSIGILVDRGQF-SRKSYVTSKNRYNIDVGVLFIGGKDDREALSLVKRMKYNPRVRVT 674

Query: 667 VLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVN 726
           V++L    +    S+W+ +LD+E LKD K +   N  + Y E +     +    ++ +  
Sbjct: 675 VIRL--IFDHEIESEWDYILDNEGLKDLK-STESNEDILYTERIVTSVVEVVKAVQLLAE 734

Query: 727 EFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 770
           E+DLM+VGR   + S   +GL+EW E PELGV+GDL+A+ D N++ SVLV+QQQ+
Sbjct: 735 EYDLMVVGRDHDMTSQDLSGLTEWVELPELGVIGDLLAARDLNSKVSVLVVQQQQ 780

BLAST of MC10g0085 vs. ExPASy Swiss-Prot
Match: Q58P69 (Cation/H(+) antiporter 10 OS=Arabidopsis thaliana OX=3702 GN=CHX10 PE=2 SV=2)

HSP 1 Score: 420.6 bits (1080), Expect = 3.8e-116
Identity = 262/778 (33.68%), Postives = 415/778 (53.34%), Query Frame = 0

Query: 7   WRVSSDGIWGKFSRPQWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSKISSQIITGL 66
           + +SS G W     P      SLPLLE+Q+ L F  + + H+ L+  G+S+I+S +I G+
Sbjct: 15  FNISSQGFWDNLKSPDVVFGYSLPLLEIQIILIFFCIVMSHMFLRCIGISQIASYMIAGI 74

Query: 67  VFGCSWGQLNNKKLEFFRIN-SEEIIGLLAC---FGYMLFLFITAVKMDVRRTMKTGRKA 126
           V G     +  K      ++ + + I  L C   FG ++F F+  V+   R    +G+  
Sbjct: 75  VLGPQLFDVLEKSSGKLSVDPALDGIAALRCISVFGTLMFTFLMTVRTSRRVAFHSGKLP 134

Query: 127 WIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPL---------MVSIHSFISFPVIAS 186
            +IGI S F P+  GL   ++F D +   + + +PL         +V   S I  P    
Sbjct: 135 VVIGIVSFFAPLF-GLGFQNFFSDNI---DPHYMPLTKALGERTAIVITQSSILLPSTTY 194

Query: 187 LLSELKLVSTELGRLGMSSALIADLFSQFVMGTAN-QIRISRTSITLGYFSKAGIMVQIC 246
           +L ELK++++ELGRL +S+ +I D+   F M  A+ Q      S    Y     +++   
Sbjct: 195 ILLELKIINSELGRLALSACVINDILGIFSMIVASIQATYIHVSHATAYRDTVAVIIFFL 254

Query: 247 LILFVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLGLAI 306
           ++  V +P V W++ +TPE KPV     H + +  L SA          I GP ++G+ I
Sbjct: 255 VVFLVFKPMVQWVIDRTPEDKPVEDMYIHAVIITALASAAYFVFFNMKYILGPLMIGIII 314

Query: 307 PDGATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAADARVNIVLSLVTY 366
           P+G   LG +L  K E    + FLP+ +   +++ D ++I       D   NI L+ +  
Sbjct: 315 PEG-PPLGSALEAKFERLTMNVFLPISITFSAMRCDGARILSQFN--DIFFNIFLTFLIL 374

Query: 367 GVKLVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFIVFL 426
            +KLV C    L  KLPL +S  +S ILS K   +      +     I++   ++ I++ 
Sbjct: 375 VIKLVACLAPCLYYKLPLSESLAVSFILSYKSFADFVLYEAVLDDTYISQATYSFLILYS 434

Query: 427 LIVATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLLN-L 486
           L+ A  VP  ++ + DP+R +  YQ R+I+HL  NS+LR+L C+HK E++   I  L  L
Sbjct: 435 LLNAGIVPTVLRRMYDPRRKYVNYQKRDILHLERNSDLRILTCLHKPENVSETIAFLQLL 494

Query: 487 SCPTPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNSAG 546
           S P    P+AV  LHL++LVG+  PI +SH ++   ++  +Y     L+F  F  + S  
Sbjct: 495 SSPNLDFPIAVTVLHLVKLVGQINPIIVSHDKKLKRLNKDSYIHTANLAFRQFVLE-SLE 554

Query: 547 SVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSV 606
           SV    FT  +    M  DIC LALDK TS+I++P  R WT DGL + ++  +R+LN S+
Sbjct: 555 SVTVTTFTAFSHENLMHEDICTLALDKTTSMIVVPSGRKWTVDGLFESDNTAIRHLNQSL 614

Query: 607 LQKADCSVAIFADKGHLGSVASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTI 666
           L +A CS+ I  D+G   S  S+ +S  ++   V ++++GG DDREA+S  KRM   P I
Sbjct: 615 LDRAPCSIGILVDRGQF-SRKSIVTSKKRYIIDVGVLFIGGKDDREALSLVKRMKNNPRI 674

Query: 667 ELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRK 726
            +TV++L    +    S W+ +LD+E LKD K +   N  + Y E +     +    ++ 
Sbjct: 675 RVTVIRL--VFDHEIESDWDYILDNEGLKDLK-STEDNKDIDYIERIVTSSVEVVKAVQL 734

Query: 727 IVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 770
           +  E+DLM+VGR   + S   +GL EW E PELGV+GDL+A+ D +++ SVLV+QQQ+
Sbjct: 735 LAEEYDLMVVGRDHDMTSQDLSGLMEWVELPELGVIGDLLAARDLSSKVSVLVVQQQQ 780

BLAST of MC10g0085 vs. NCBI nr
Match: XP_022141218.1 (cation/H(+) antiporter 3-like [Momordica charantia])

HSP 1 Score: 1345 bits (3482), Expect = 0.0
Identity = 671/770 (87.14%), Postives = 714/770 (92.73%), Query Frame = 0

Query: 1   CLFVQPWRVSSDGIWGKFSRPQWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSKISS 60
           CL + P RV S+GIWG  S  +WWLNNSLPLLELQLF FFLLMSIIHLL KPFGV KISS
Sbjct: 26  CLLMPP-RVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFFLLMSIIHLLFKPFGVPKISS 85

Query: 61  QIITGLVFGCSWGQLNNKKLEFFRINSEEIIGLLACFGYMLFLFITAVKMDVRRTMKTGR 120
           QII GLV GCSWG L++ K   FRINSEEI+GLL+ FGYMLFLFITA KMDVR  +KTGR
Sbjct: 86  QIIAGLVVGCSWGPLDDTKFRLFRINSEEILGLLSYFGYMLFLFITAAKMDVRMILKTGR 145

Query: 121 KAWIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPLMVSIHSFISFPVIASLLSELKL 180
           KAWIIGIPS  +P+ICG +VGSYF DGLS AEKN VP++VSI SFISFPVIASLLSELK+
Sbjct: 146 KAWIIGIPSTLIPIICGFWVGSYFLDGLSPAEKNRVPVIVSIQSFISFPVIASLLSELKM 205

Query: 181 VSTELGRLGMSSALIADLFSQFVMGTANQIRISRTSITLGYFSKAGIMVQICLILFVLRP 240
           VSTELGRLG+SSALIADL  QFVM TANQIRISR++ITLGYF+KAGIMVQ CLI+FVLRP
Sbjct: 206 VSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLGYFNKAGIMVQFCLIIFVLRP 265

Query: 241 SVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLGLAIPDGATQLG 300
           SVLW+LKQTPEGKPVSR TTHG+F+ VL+SAV+SALLGQPA+FGPYLLGLAIPDGA+ LG
Sbjct: 266 SVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQPAVFGPYLLGLAIPDGASGLG 325

Query: 301 FSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAAD-ARVNIVLSLVTYGVKLVCC 360
           FSLVEK+ECFVSDFF+PLFVITCSLQ D+SK+F+V  AA  AR+NI+++LVTYGVKLVCC
Sbjct: 326 FSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAAFARINIIITLVTYGVKLVCC 385

Query: 361 FLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFIVFLLIVATFV 420
           FLCSL CKLPLRD FVLSLILSCKGV EL F  +  QY++IN GILAWFI+FLL +ATFV
Sbjct: 386 FLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKIINHGILAWFILFLLTIATFV 445

Query: 421 PLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLLNLSCPTPQNP 480
           PLTVKWLTDPKRTH  +QNRNIMHLSPNSELR+LPCIHKNEHIYGLIHLLNLSCPTPQNP
Sbjct: 446 PLTVKWLTDPKRTHSSHQNRNIMHLSPNSELRILPCIHKNEHIYGLIHLLNLSCPTPQNP 505

Query: 481 LAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNSAGSVYAECFT 540
           LAVYALHLIELVGRTTPIFISHRQEN AI+DQ YSENVTLSFDHFEKDNSAGSVYAECFT
Sbjct: 506 LAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTLSFDHFEKDNSAGSVYAECFT 565

Query: 541 VITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQKADCSV 600
           VITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQKADCSV
Sbjct: 566 VITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQKADCSV 625

Query: 601 AIFADKGHLGSVASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELTVLKLG 660
           AIFADKGHLGS ASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELTVLKLG
Sbjct: 626 AIFADKGHLGSXASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELTVLKLG 685

Query: 661 SPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVNEFDLM 720
           SPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVNEFDLM
Sbjct: 686 SPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVNEFDLM 745

Query: 721 IVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 769
           IVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK
Sbjct: 746 IVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 794

BLAST of MC10g0085 vs. NCBI nr
Match: XP_022141141.1 (cation/H(+) antiporter 4-like [Momordica charantia])

HSP 1 Score: 1300 bits (3363), Expect = 0.0
Identity = 659/660 (99.85%), Postives = 659/660 (99.85%), Query Frame = 0

Query: 110 MDVRRTMKTGRKAWIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPLMVSIHSFISFP 169
           MDVRRTMKTGRKAWIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPLMVSIHSFISFP
Sbjct: 1   MDVRRTMKTGRKAWIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPLMVSIHSFISFP 60

Query: 170 VIASLLSELKLVSTELGRLGMSSALIADLFSQFVMGTANQIRISRTSITLGYFSKAGIMV 229
           VIASLLSELKLVSTELGRLGMSSALIADLFSQFVMGTANQIRISRTSITLGYFSKAGIMV
Sbjct: 61  VIASLLSELKLVSTELGRLGMSSALIADLFSQFVMGTANQIRISRTSITLGYFSKAGIMV 120

Query: 230 QICLILFVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLG 289
           QICLILFVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLG
Sbjct: 121 QICLILFVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLG 180

Query: 290 LAIPDGATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAADARVNIVLSL 349
           LAIPDGATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAADARVNIVLSL
Sbjct: 181 LAIPDGATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAADARVNIVLSL 240

Query: 350 VTYGVKLVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFI 409
           VTYGVKLVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFI
Sbjct: 241 VTYGVKLVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFI 300

Query: 410 VFLLIVATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLL 469
           VFLLIVATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLL
Sbjct: 301 VFLLIVATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLL 360

Query: 470 NLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNS 529
           NLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNS
Sbjct: 361 NLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNS 420

Query: 530 AGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNC 589
           AGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNC
Sbjct: 421 AGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNC 480

Query: 590 SVLQKADCSVAIFADKGHLGSVASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEP 649
           SVLQKADCSVAIFADKGHLGS ASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEP
Sbjct: 481 SVLQKADCSVAIFADKGHLGSXASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEP 540

Query: 650 TIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTL 709
           TIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTL
Sbjct: 541 TIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTL 600

Query: 710 RKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 769
           RKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK
Sbjct: 601 RKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 660

BLAST of MC10g0085 vs. NCBI nr
Match: KGN57865.1 (hypothetical protein Csa_011167 [Cucumis sativus])

HSP 1 Score: 1071 bits (2770), Expect = 0.0
Identity = 535/771 (69.39%), Postives = 639/771 (82.88%), Query Frame = 0

Query: 1   CLFVQPWRVSSDGIWGKFSRPQWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSKISS 60
           C F  P+ ++S G+W    RPQWWLNNSLPLLELQL LF   M+IIH LLK  GVSK+SS
Sbjct: 10  CTFHPPF-INSGGLWINIHRPQWWLNNSLPLLELQLVLFCFFMAIIHFLLKRSGVSKLSS 69

Query: 61  QIITGLVFGCSWGQLNNKKLEFFRINSEEIIGLLACFGYMLFLFITAVKMDVRRTMKTGR 120
           QIITGL+FGCSWG+LN  K E FR+ SEEI+GL + F YMLF+FITAVKMDV  T+KTG+
Sbjct: 70  QIITGLIFGCSWGKLNKGKFELFRVESEEILGLFSYFAYMLFMFITAVKMDVSMTLKTGK 129

Query: 121 KAWIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPLMVSIHSFISFPVIASLLSELKL 180
           +AWIIG+PS+ +P+ CGL V S+  +GL+ +E   +PLMVS+ S ISFPVIA LL+ELK+
Sbjct: 130 RAWIIGLPSILLPLTCGLLVSSFLLEGLTISEIRKLPLMVSMQSMISFPVIACLLNELKI 189

Query: 181 VSTELGRLGMSSALIADLFSQFVMGTANQIRISRTSITLGYFSKAGIMVQICLILFVLRP 240
           VSTELGRLG+SSAL+AD+FSQ  +  ANQIRISR +   GY+S  G+ VQ+ L+ F+ RP
Sbjct: 190 VSTELGRLGLSSALVADMFSQCAVAIANQIRISRKNAAKGYYSIGGLCVQVFLVSFLFRP 249

Query: 241 SVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLGLAIPDGATQLG 300
            VLW++KQT EGKPVSRGTT  +FL VL+SAV S LLGQPAI GPYLLGL++ DG    G
Sbjct: 250 VVLWIMKQTTEGKPVSRGTTQTVFLVVLLSAVTSTLLGQPAIVGPYLLGLSLTDGGPT-G 309

Query: 301 FSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAAD-ARVNIVLSLVTYGVKLVCC 360
           FSL+EKLECFVSDFF+P+FVITC+LQVD+S+I +V    +  RVNI+L+ VTY  K VC 
Sbjct: 310 FSLIEKLECFVSDFFMPVFVITCALQVDLSRILLVAALDNYTRVNIILAFVTYVTKFVCT 369

Query: 361 FLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFIVFLLIVATFV 420
           FL SL C+L  R+S +LSLILS KGV EL+FC++ T+Y +++RGILAWF VFLL++ATFV
Sbjct: 370 FLTSLYCQLSFRESLMLSLILSSKGVVELSFCTLFTEYNILSRGILAWFTVFLLLIATFV 429

Query: 421 PLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLLNLSCPTPQNP 480
           P+ +K+L D  +     QNRNIMHLS NSELRVL C+H NE+IYG IHLLN+SCPT +NP
Sbjct: 430 PMVLKFLNDISKLQASNQNRNIMHLSQNSELRVLACVHTNENIYGFIHLLNISCPTQENP 489

Query: 481 LAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNSAGSVYAECFT 540
           +AVYALHLIELVGRT P+FISHR EN  I DQTYSEN+ LSFDHFEK+NS GSVYAECFT
Sbjct: 490 VAVYALHLIELVGRTAPVFISHRIENKPIGDQTYSENMLLSFDHFEKENS-GSVYAECFT 549

Query: 541 VITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQKADCSV 600
            I+PHKFM N+ICKLA+DK+TSLIILPFH TWT+DGL+DQEDN +RNLNCSV++KA CSV
Sbjct: 550 SISPHKFMHNEICKLAMDKITSLIILPFHITWTSDGLVDQEDNTMRNLNCSVIEKAPCSV 609

Query: 601 AIFADKGHLGSVASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELTVLKLG 660
           AI ADKGHLGS+ASMASSG +  Y+VC+IYLGGSDDREAISFAKR+AK+  IELTVLKLG
Sbjct: 610 AILADKGHLGSIASMASSGVRCEYTVCVIYLGGSDDREAISFAKRLAKDIKIELTVLKLG 669

Query: 661 SPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVNEFDLM 720
           S +ED  +SKWE MLDSEV+KDFKM CLG+G V++ EEV +DGPQTAL LR++VN+FDLM
Sbjct: 670 SSVEDNGTSKWEKMLDSEVIKDFKMTCLGDGRVKFLEEVSEDGPQTALRLRELVNDFDLM 729

Query: 721 IVGRRDGLES-PQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 769
           IVGRR G+ES PQT+GLSEWNEFPELGVLGDLIASLD NTRTSVLVIQQQK
Sbjct: 730 IVGRRKGMESSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQQQK 777

BLAST of MC10g0085 vs. NCBI nr
Match: XP_016899409.1 (PREDICTED: cation/H(+) antiporter 4-like [Cucumis melo])

HSP 1 Score: 1070 bits (2767), Expect = 0.0
Identity = 534/771 (69.26%), Postives = 639/771 (82.88%), Query Frame = 0

Query: 1   CLFVQPWRVSSDGIWGKFSRPQWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSKISS 60
           C F  P+ V+SDG+W   +R +WWLNNSLPLLELQL LF   M+IIH LLK  GVSKISS
Sbjct: 20  CTFHPPF-VNSDGLWTNINRHEWWLNNSLPLLELQLVLFCFFMAIIHFLLKRSGVSKISS 79

Query: 61  QIITGLVFGCSWGQLNNKKLEFFRINSEEIIGLLACFGYMLFLFITAVKMDVRRTMKTGR 120
           QIITGL+FGCSWG+L+  K + FR+ SEEI+GL + F YMLF+FITAVKMDV  TMKTG+
Sbjct: 80  QIITGLIFGCSWGKLDKGKFKLFRVESEEILGLFSYFAYMLFMFITAVKMDVSMTMKTGK 139

Query: 121 KAWIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPLMVSIHSFISFPVIASLLSELKL 180
           +AWIIG+PS+ +P+ CGL V S+  +GL+ +E   +PLMVS+ S ISFPVIA LL+ELK+
Sbjct: 140 RAWIIGLPSILMPLTCGLLVSSFLLEGLTISEIRKLPLMVSMQSMISFPVIACLLNELKI 199

Query: 181 VSTELGRLGMSSALIADLFSQFVMGTANQIRISRTSITLGYFSKAGIMVQICLILFVLRP 240
           VSTELGRLG+SSAL+AD+FSQ  +  ANQIRISR +   GY+S  G+ VQ+ ++ F+ RP
Sbjct: 200 VSTELGRLGLSSALVADMFSQCAVAIANQIRISRKNAGKGYYSIGGLCVQVFMVSFLFRP 259

Query: 241 SVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLGLAIPDGATQLG 300
           +VLW++KQTPEGKP SRGTT  +FL VL+SAV S LLGQPA+ GPYLLGL++ DG   +G
Sbjct: 260 AVLWIMKQTPEGKPASRGTTQTVFLVVLLSAVTSTLLGQPAVVGPYLLGLSLTDGGP-MG 319

Query: 301 FSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAAD-ARVNIVLSLVTYGVKLVCC 360
           FSL+EKLECFVSDFF+P+FVITC+LQVD+S+I +V    D  RVNI+L+ VTY  +  C 
Sbjct: 320 FSLIEKLECFVSDFFMPVFVITCALQVDLSQILLVAVVDDFTRVNIILAFVTYVTQFFCT 379

Query: 361 FLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFIVFLLIVATFV 420
           FL SL C+L  RDS +LSLIL  KGV EL+FC++ T+Y +I+RGILAWF VFLL++ATFV
Sbjct: 380 FLTSLYCQLSFRDSLMLSLILGSKGVVELSFCTLFTEYNIISRGILAWFTVFLLLIATFV 439

Query: 421 PLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLLNLSCPTPQNP 480
           P+ +K L D  +     QNRNIMHLS NSE RVL C+HKNE+IYG IHLLN+SCPT +NP
Sbjct: 440 PIMLKCLNDISKLQASNQNRNIMHLSQNSEFRVLACVHKNENIYGFIHLLNISCPTLENP 499

Query: 481 LAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNSAGSVYAECFT 540
           +AVYALHLIELVGRTTP+FISHR EN  I DQTYSEN+ LSFDHFEKDNS GSVYAECFT
Sbjct: 500 VAVYALHLIELVGRTTPVFISHRIENKPIGDQTYSENMLLSFDHFEKDNS-GSVYAECFT 559

Query: 541 VITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQKADCSV 600
            I+PHKFM NDICKLA+DK+TSLIILPFH TWT+DGLIDQEDN +RNLNC V++KA CSV
Sbjct: 560 SISPHKFMHNDICKLAMDKITSLIILPFHITWTSDGLIDQEDNTMRNLNCCVIEKAPCSV 619

Query: 601 AIFADKGHLGSVASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELTVLKLG 660
           AI ADKGHLGS+ASMASSG +  Y+VC+IY+GGSDDREAISFAKR+AK+  IELTVLKLG
Sbjct: 620 AILADKGHLGSIASMASSGVKCEYTVCVIYMGGSDDREAISFAKRLAKDIKIELTVLKLG 679

Query: 661 SPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVNEFDLM 720
           S +ED  +SKWE MLDSEV+KDFKM CLG+G V++ EEV +DGPQTAL LR++VN+FDLM
Sbjct: 680 SSVEDNGTSKWEKMLDSEVIKDFKMTCLGDGRVKFLEEVSEDGPQTALRLRELVNDFDLM 739

Query: 721 IVGRRDGLES-PQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 769
           IVGRR G+ES PQT+GLSEWNEFPELG+LGDLIASLD NTRTSVLVIQQQK
Sbjct: 740 IVGRRKGMESSPQTSGLSEWNEFPELGILGDLIASLDINTRTSVLVIQQQK 787

BLAST of MC10g0085 vs. NCBI nr
Match: TYK03148.1 (cation/H(+) antiporter 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 1069 bits (2764), Expect = 0.0
Identity = 534/771 (69.26%), Postives = 638/771 (82.75%), Query Frame = 0

Query: 1   CLFVQPWRVSSDGIWGKFSRPQWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSKISS 60
           C F  P+ V+SDG+W    R QWWLNNSLPLLELQL LF   M+IIH LLK  GVSKISS
Sbjct: 20  CTFHPPF-VNSDGLWTNIHRHQWWLNNSLPLLELQLVLFCFFMAIIHFLLKRSGVSKISS 79

Query: 61  QIITGLVFGCSWGQLNNKKLEFFRINSEEIIGLLACFGYMLFLFITAVKMDVRRTMKTGR 120
           QIITGL+FGCSWG+L+  K + FR+ SEEI+GL + F YMLF+FITAVKMDV  T+KTG+
Sbjct: 80  QIITGLIFGCSWGKLDKGKFKLFRVESEEILGLFSYFAYMLFMFITAVKMDVSMTVKTGK 139

Query: 121 KAWIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPLMVSIHSFISFPVIASLLSELKL 180
           +AWIIG+PS+ +P+ CGL V S+  +GL+ +E   +PLMVS+ S ISFPVIA LL+ELK+
Sbjct: 140 RAWIIGLPSILMPLTCGLLVSSFLLEGLTISEIRKLPLMVSMQSMISFPVIACLLNELKI 199

Query: 181 VSTELGRLGMSSALIADLFSQFVMGTANQIRISRTSITLGYFSKAGIMVQICLILFVLRP 240
           VSTELGRLG+SSAL+AD+FSQ  +  ANQIRISR +   GY+S  G+ VQ+ ++ F+ RP
Sbjct: 200 VSTELGRLGLSSALVADMFSQCAVAIANQIRISRKNAGKGYYSIGGLCVQVFMVSFLFRP 259

Query: 241 SVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLGLAIPDGATQLG 300
           +VLW++KQTPEGKP SRGTT  +FL VL+SAV S LLGQPA+ GPYLLGL++ DG   +G
Sbjct: 260 AVLWIMKQTPEGKPASRGTTQTVFLVVLLSAVTSTLLGQPAVVGPYLLGLSLTDGGP-MG 319

Query: 301 FSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAAD-ARVNIVLSLVTYGVKLVCC 360
           FSL+EKLECFVSDFF+P+FVITC+LQVD+S+I +V    D  RVNI+L+ VTY  +  C 
Sbjct: 320 FSLIEKLECFVSDFFMPVFVITCALQVDLSQILLVAVVDDFTRVNIILAFVTYVTQFFCT 379

Query: 361 FLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFIVFLLIVATFV 420
           FL SL C+L  RDS +LSLIL  KGV EL+FC++ T+Y +I+RGILAWF VFLL++ATFV
Sbjct: 380 FLTSLYCQLSFRDSLMLSLILGSKGVVELSFCTLFTEYNIISRGILAWFTVFLLLIATFV 439

Query: 421 PLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLLNLSCPTPQNP 480
           P+ +K L D  +     QNRNIMHLS NSE RVL C+HKNE+IYG IHLLN+SCPT +NP
Sbjct: 440 PIMLKCLNDISKLQASNQNRNIMHLSQNSEFRVLACVHKNENIYGFIHLLNISCPTLENP 499

Query: 481 LAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNSAGSVYAECFT 540
           +AVYALHLIELVGRTTP+FISHR EN  I DQTYSEN+ LSFDHFEKDNS GSVYAECFT
Sbjct: 500 VAVYALHLIELVGRTTPVFISHRIENKPIGDQTYSENMLLSFDHFEKDNS-GSVYAECFT 559

Query: 541 VITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQKADCSV 600
            I+PHKFM NDICKLA+DK+TSLIILPFH TWT+DGLIDQEDN +RNLNC V++KA CSV
Sbjct: 560 SISPHKFMHNDICKLAMDKITSLIILPFHITWTSDGLIDQEDNTMRNLNCCVIEKAPCSV 619

Query: 601 AIFADKGHLGSVASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELTVLKLG 660
           AI ADKGHLGS+ASMASSG +  Y+VC+IY+GGSDDREAISFAKR+AK+  IELTVLKLG
Sbjct: 620 AILADKGHLGSIASMASSGVKCEYTVCVIYMGGSDDREAISFAKRLAKDIKIELTVLKLG 679

Query: 661 SPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVNEFDLM 720
           S +ED  +SKWE MLDSEV+KDFKM CLG+G V++ EEV +DGPQTAL LR++VN+FDLM
Sbjct: 680 SSVEDNGTSKWEKMLDSEVIKDFKMTCLGDGRVKFLEEVSEDGPQTALRLRELVNDFDLM 739

Query: 721 IVGRRDGLES-PQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 769
           IVGRR G+ES PQT+GLSEWNEFPELG+LGDLIASLD NTRTSVLVIQQQK
Sbjct: 740 IVGRRKGMESSPQTSGLSEWNEFPELGILGDLIASLDINTRTSVLVIQQQK 787

BLAST of MC10g0085 vs. ExPASy TrEMBL
Match: A0A6J1CJU7 (cation/H(+) antiporter 3-like OS=Momordica charantia OX=3673 GN=LOC111011670 PE=4 SV=1)

HSP 1 Score: 1345 bits (3482), Expect = 0.0
Identity = 671/770 (87.14%), Postives = 714/770 (92.73%), Query Frame = 0

Query: 1   CLFVQPWRVSSDGIWGKFSRPQWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSKISS 60
           CL + P RV S+GIWG  S  +WWLNNSLPLLELQLF FFLLMSIIHLL KPFGV KISS
Sbjct: 26  CLLMPP-RVGSNGIWGNSSTSEWWLNNSLPLLELQLFFFFLLMSIIHLLFKPFGVPKISS 85

Query: 61  QIITGLVFGCSWGQLNNKKLEFFRINSEEIIGLLACFGYMLFLFITAVKMDVRRTMKTGR 120
           QII GLV GCSWG L++ K   FRINSEEI+GLL+ FGYMLFLFITA KMDVR  +KTGR
Sbjct: 86  QIIAGLVVGCSWGPLDDTKFRLFRINSEEILGLLSYFGYMLFLFITAAKMDVRMILKTGR 145

Query: 121 KAWIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPLMVSIHSFISFPVIASLLSELKL 180
           KAWIIGIPS  +P+ICG +VGSYF DGLS AEKN VP++VSI SFISFPVIASLLSELK+
Sbjct: 146 KAWIIGIPSTLIPIICGFWVGSYFLDGLSPAEKNRVPVIVSIQSFISFPVIASLLSELKM 205

Query: 181 VSTELGRLGMSSALIADLFSQFVMGTANQIRISRTSITLGYFSKAGIMVQICLILFVLRP 240
           VSTELGRLG+SSALIADL  QFVM TANQIRISR++ITLGYF+KAGIMVQ CLI+FVLRP
Sbjct: 206 VSTELGRLGLSSALIADLLGQFVMATANQIRISRSNITLGYFNKAGIMVQFCLIIFVLRP 265

Query: 241 SVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLGLAIPDGATQLG 300
           SVLW+LKQTPEGKPVSR TTHG+F+ VL+SAV+SALLGQPA+FGPYLLGLAIPDGA+ LG
Sbjct: 266 SVLWILKQTPEGKPVSRATTHGVFILVLLSAVSSALLGQPAVFGPYLLGLAIPDGASGLG 325

Query: 301 FSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAAD-ARVNIVLSLVTYGVKLVCC 360
           FSLVEK+ECFVSDFF+PLFVITCSLQ D+SK+F+V  AA  AR+NI+++LVTYGVKLVCC
Sbjct: 326 FSLVEKMECFVSDFFMPLFVITCSLQADVSKMFLVFAAAAFARINIIITLVTYGVKLVCC 385

Query: 361 FLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFIVFLLIVATFV 420
           FLCSL CKLPLRD FVLSLILSCKGV EL F  +  QY++IN GILAWFI+FLL +ATFV
Sbjct: 386 FLCSLYCKLPLRDCFVLSLILSCKGVVELGFYLVTHQYKIINHGILAWFILFLLTIATFV 445

Query: 421 PLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLLNLSCPTPQNP 480
           PLTVKWLTDPKRTH  +QNRNIMHLSPNSELR+LPCIHKNEHIYGLIHLLNLSCPTPQNP
Sbjct: 446 PLTVKWLTDPKRTHSSHQNRNIMHLSPNSELRILPCIHKNEHIYGLIHLLNLSCPTPQNP 505

Query: 481 LAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNSAGSVYAECFT 540
           LAVYALHLIELVGRTTPIFISHRQEN AI+DQ YSENVTLSFDHFEKDNSAGSVYAECFT
Sbjct: 506 LAVYALHLIELVGRTTPIFISHRQENKAIADQPYSENVTLSFDHFEKDNSAGSVYAECFT 565

Query: 541 VITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQKADCSV 600
           VITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQKADCSV
Sbjct: 566 VITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQKADCSV 625

Query: 601 AIFADKGHLGSVASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELTVLKLG 660
           AIFADKGHLGS ASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELTVLKLG
Sbjct: 626 AIFADKGHLGSXASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELTVLKLG 685

Query: 661 SPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVNEFDLM 720
           SPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVNEFDLM
Sbjct: 686 SPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVNEFDLM 745

Query: 721 IVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 769
           IVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK
Sbjct: 746 IVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 794

BLAST of MC10g0085 vs. ExPASy TrEMBL
Match: A0A6J1CI53 (cation/H(+) antiporter 4-like OS=Momordica charantia OX=3673 GN=LOC111011605 PE=4 SV=1)

HSP 1 Score: 1300 bits (3363), Expect = 0.0
Identity = 659/660 (99.85%), Postives = 659/660 (99.85%), Query Frame = 0

Query: 110 MDVRRTMKTGRKAWIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPLMVSIHSFISFP 169
           MDVRRTMKTGRKAWIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPLMVSIHSFISFP
Sbjct: 1   MDVRRTMKTGRKAWIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPLMVSIHSFISFP 60

Query: 170 VIASLLSELKLVSTELGRLGMSSALIADLFSQFVMGTANQIRISRTSITLGYFSKAGIMV 229
           VIASLLSELKLVSTELGRLGMSSALIADLFSQFVMGTANQIRISRTSITLGYFSKAGIMV
Sbjct: 61  VIASLLSELKLVSTELGRLGMSSALIADLFSQFVMGTANQIRISRTSITLGYFSKAGIMV 120

Query: 230 QICLILFVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLG 289
           QICLILFVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLG
Sbjct: 121 QICLILFVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLG 180

Query: 290 LAIPDGATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAADARVNIVLSL 349
           LAIPDGATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAADARVNIVLSL
Sbjct: 181 LAIPDGATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAADARVNIVLSL 240

Query: 350 VTYGVKLVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFI 409
           VTYGVKLVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFI
Sbjct: 241 VTYGVKLVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFI 300

Query: 410 VFLLIVATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLL 469
           VFLLIVATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLL
Sbjct: 301 VFLLIVATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLL 360

Query: 470 NLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNS 529
           NLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNS
Sbjct: 361 NLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNS 420

Query: 530 AGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNC 589
           AGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNC
Sbjct: 421 AGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNC 480

Query: 590 SVLQKADCSVAIFADKGHLGSVASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEP 649
           SVLQKADCSVAIFADKGHLGS ASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEP
Sbjct: 481 SVLQKADCSVAIFADKGHLGSXASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEP 540

Query: 650 TIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTL 709
           TIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTL
Sbjct: 541 TIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTL 600

Query: 710 RKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 769
           RKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK
Sbjct: 601 RKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 660

BLAST of MC10g0085 vs. ExPASy TrEMBL
Match: A0A0A0L7A8 (Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G355570 PE=4 SV=1)

HSP 1 Score: 1071 bits (2770), Expect = 0.0
Identity = 535/771 (69.39%), Postives = 639/771 (82.88%), Query Frame = 0

Query: 1   CLFVQPWRVSSDGIWGKFSRPQWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSKISS 60
           C F  P+ ++S G+W    RPQWWLNNSLPLLELQL LF   M+IIH LLK  GVSK+SS
Sbjct: 10  CTFHPPF-INSGGLWINIHRPQWWLNNSLPLLELQLVLFCFFMAIIHFLLKRSGVSKLSS 69

Query: 61  QIITGLVFGCSWGQLNNKKLEFFRINSEEIIGLLACFGYMLFLFITAVKMDVRRTMKTGR 120
           QIITGL+FGCSWG+LN  K E FR+ SEEI+GL + F YMLF+FITAVKMDV  T+KTG+
Sbjct: 70  QIITGLIFGCSWGKLNKGKFELFRVESEEILGLFSYFAYMLFMFITAVKMDVSMTLKTGK 129

Query: 121 KAWIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPLMVSIHSFISFPVIASLLSELKL 180
           +AWIIG+PS+ +P+ CGL V S+  +GL+ +E   +PLMVS+ S ISFPVIA LL+ELK+
Sbjct: 130 RAWIIGLPSILLPLTCGLLVSSFLLEGLTISEIRKLPLMVSMQSMISFPVIACLLNELKI 189

Query: 181 VSTELGRLGMSSALIADLFSQFVMGTANQIRISRTSITLGYFSKAGIMVQICLILFVLRP 240
           VSTELGRLG+SSAL+AD+FSQ  +  ANQIRISR +   GY+S  G+ VQ+ L+ F+ RP
Sbjct: 190 VSTELGRLGLSSALVADMFSQCAVAIANQIRISRKNAAKGYYSIGGLCVQVFLVSFLFRP 249

Query: 241 SVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLGLAIPDGATQLG 300
            VLW++KQT EGKPVSRGTT  +FL VL+SAV S LLGQPAI GPYLLGL++ DG    G
Sbjct: 250 VVLWIMKQTTEGKPVSRGTTQTVFLVVLLSAVTSTLLGQPAIVGPYLLGLSLTDGGPT-G 309

Query: 301 FSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAAD-ARVNIVLSLVTYGVKLVCC 360
           FSL+EKLECFVSDFF+P+FVITC+LQVD+S+I +V    +  RVNI+L+ VTY  K VC 
Sbjct: 310 FSLIEKLECFVSDFFMPVFVITCALQVDLSRILLVAALDNYTRVNIILAFVTYVTKFVCT 369

Query: 361 FLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFIVFLLIVATFV 420
           FL SL C+L  R+S +LSLILS KGV EL+FC++ T+Y +++RGILAWF VFLL++ATFV
Sbjct: 370 FLTSLYCQLSFRESLMLSLILSSKGVVELSFCTLFTEYNILSRGILAWFTVFLLLIATFV 429

Query: 421 PLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLLNLSCPTPQNP 480
           P+ +K+L D  +     QNRNIMHLS NSELRVL C+H NE+IYG IHLLN+SCPT +NP
Sbjct: 430 PMVLKFLNDISKLQASNQNRNIMHLSQNSELRVLACVHTNENIYGFIHLLNISCPTQENP 489

Query: 481 LAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNSAGSVYAECFT 540
           +AVYALHLIELVGRT P+FISHR EN  I DQTYSEN+ LSFDHFEK+NS GSVYAECFT
Sbjct: 490 VAVYALHLIELVGRTAPVFISHRIENKPIGDQTYSENMLLSFDHFEKENS-GSVYAECFT 549

Query: 541 VITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQKADCSV 600
            I+PHKFM N+ICKLA+DK+TSLIILPFH TWT+DGL+DQEDN +RNLNCSV++KA CSV
Sbjct: 550 SISPHKFMHNEICKLAMDKITSLIILPFHITWTSDGLVDQEDNTMRNLNCSVIEKAPCSV 609

Query: 601 AIFADKGHLGSVASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELTVLKLG 660
           AI ADKGHLGS+ASMASSG +  Y+VC+IYLGGSDDREAISFAKR+AK+  IELTVLKLG
Sbjct: 610 AILADKGHLGSIASMASSGVRCEYTVCVIYLGGSDDREAISFAKRLAKDIKIELTVLKLG 669

Query: 661 SPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVNEFDLM 720
           S +ED  +SKWE MLDSEV+KDFKM CLG+G V++ EEV +DGPQTAL LR++VN+FDLM
Sbjct: 670 SSVEDNGTSKWEKMLDSEVIKDFKMTCLGDGRVKFLEEVSEDGPQTALRLRELVNDFDLM 729

Query: 721 IVGRRDGLES-PQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 769
           IVGRR G+ES PQT+GLSEWNEFPELGVLGDLIASLD NTRTSVLVIQQQK
Sbjct: 730 IVGRRKGMESSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQQQK 777

BLAST of MC10g0085 vs. ExPASy TrEMBL
Match: A0A1S4DTU4 (cation/H(+) antiporter 4-like OS=Cucumis melo OX=3656 GN=LOC103485746 PE=4 SV=1)

HSP 1 Score: 1070 bits (2767), Expect = 0.0
Identity = 534/771 (69.26%), Postives = 639/771 (82.88%), Query Frame = 0

Query: 1   CLFVQPWRVSSDGIWGKFSRPQWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSKISS 60
           C F  P+ V+SDG+W   +R +WWLNNSLPLLELQL LF   M+IIH LLK  GVSKISS
Sbjct: 20  CTFHPPF-VNSDGLWTNINRHEWWLNNSLPLLELQLVLFCFFMAIIHFLLKRSGVSKISS 79

Query: 61  QIITGLVFGCSWGQLNNKKLEFFRINSEEIIGLLACFGYMLFLFITAVKMDVRRTMKTGR 120
           QIITGL+FGCSWG+L+  K + FR+ SEEI+GL + F YMLF+FITAVKMDV  TMKTG+
Sbjct: 80  QIITGLIFGCSWGKLDKGKFKLFRVESEEILGLFSYFAYMLFMFITAVKMDVSMTMKTGK 139

Query: 121 KAWIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPLMVSIHSFISFPVIASLLSELKL 180
           +AWIIG+PS+ +P+ CGL V S+  +GL+ +E   +PLMVS+ S ISFPVIA LL+ELK+
Sbjct: 140 RAWIIGLPSILMPLTCGLLVSSFLLEGLTISEIRKLPLMVSMQSMISFPVIACLLNELKI 199

Query: 181 VSTELGRLGMSSALIADLFSQFVMGTANQIRISRTSITLGYFSKAGIMVQICLILFVLRP 240
           VSTELGRLG+SSAL+AD+FSQ  +  ANQIRISR +   GY+S  G+ VQ+ ++ F+ RP
Sbjct: 200 VSTELGRLGLSSALVADMFSQCAVAIANQIRISRKNAGKGYYSIGGLCVQVFMVSFLFRP 259

Query: 241 SVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLGLAIPDGATQLG 300
           +VLW++KQTPEGKP SRGTT  +FL VL+SAV S LLGQPA+ GPYLLGL++ DG   +G
Sbjct: 260 AVLWIMKQTPEGKPASRGTTQTVFLVVLLSAVTSTLLGQPAVVGPYLLGLSLTDGGP-MG 319

Query: 301 FSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAAD-ARVNIVLSLVTYGVKLVCC 360
           FSL+EKLECFVSDFF+P+FVITC+LQVD+S+I +V    D  RVNI+L+ VTY  +  C 
Sbjct: 320 FSLIEKLECFVSDFFMPVFVITCALQVDLSQILLVAVVDDFTRVNIILAFVTYVTQFFCT 379

Query: 361 FLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFIVFLLIVATFV 420
           FL SL C+L  RDS +LSLIL  KGV EL+FC++ T+Y +I+RGILAWF VFLL++ATFV
Sbjct: 380 FLTSLYCQLSFRDSLMLSLILGSKGVVELSFCTLFTEYNIISRGILAWFTVFLLLIATFV 439

Query: 421 PLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLLNLSCPTPQNP 480
           P+ +K L D  +     QNRNIMHLS NSE RVL C+HKNE+IYG IHLLN+SCPT +NP
Sbjct: 440 PIMLKCLNDISKLQASNQNRNIMHLSQNSEFRVLACVHKNENIYGFIHLLNISCPTLENP 499

Query: 481 LAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNSAGSVYAECFT 540
           +AVYALHLIELVGRTTP+FISHR EN  I DQTYSEN+ LSFDHFEKDNS GSVYAECFT
Sbjct: 500 VAVYALHLIELVGRTTPVFISHRIENKPIGDQTYSENMLLSFDHFEKDNS-GSVYAECFT 559

Query: 541 VITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQKADCSV 600
            I+PHKFM NDICKLA+DK+TSLIILPFH TWT+DGLIDQEDN +RNLNC V++KA CSV
Sbjct: 560 SISPHKFMHNDICKLAMDKITSLIILPFHITWTSDGLIDQEDNTMRNLNCCVIEKAPCSV 619

Query: 601 AIFADKGHLGSVASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELTVLKLG 660
           AI ADKGHLGS+ASMASSG +  Y+VC+IY+GGSDDREAISFAKR+AK+  IELTVLKLG
Sbjct: 620 AILADKGHLGSIASMASSGVKCEYTVCVIYMGGSDDREAISFAKRLAKDIKIELTVLKLG 679

Query: 661 SPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVNEFDLM 720
           S +ED  +SKWE MLDSEV+KDFKM CLG+G V++ EEV +DGPQTAL LR++VN+FDLM
Sbjct: 680 SSVEDNGTSKWEKMLDSEVIKDFKMTCLGDGRVKFLEEVSEDGPQTALRLRELVNDFDLM 739

Query: 721 IVGRRDGLES-PQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 769
           IVGRR G+ES PQT+GLSEWNEFPELG+LGDLIASLD NTRTSVLVIQQQK
Sbjct: 740 IVGRRKGMESSPQTSGLSEWNEFPELGILGDLIASLDINTRTSVLVIQQQK 787

BLAST of MC10g0085 vs. ExPASy TrEMBL
Match: A0A5D3BVZ8 (Cation/H(+) antiporter 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11G00240 PE=4 SV=1)

HSP 1 Score: 1069 bits (2764), Expect = 0.0
Identity = 534/771 (69.26%), Postives = 638/771 (82.75%), Query Frame = 0

Query: 1   CLFVQPWRVSSDGIWGKFSRPQWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSKISS 60
           C F  P+ V+SDG+W    R QWWLNNSLPLLELQL LF   M+IIH LLK  GVSKISS
Sbjct: 20  CTFHPPF-VNSDGLWTNIHRHQWWLNNSLPLLELQLVLFCFFMAIIHFLLKRSGVSKISS 79

Query: 61  QIITGLVFGCSWGQLNNKKLEFFRINSEEIIGLLACFGYMLFLFITAVKMDVRRTMKTGR 120
           QIITGL+FGCSWG+L+  K + FR+ SEEI+GL + F YMLF+FITAVKMDV  T+KTG+
Sbjct: 80  QIITGLIFGCSWGKLDKGKFKLFRVESEEILGLFSYFAYMLFMFITAVKMDVSMTVKTGK 139

Query: 121 KAWIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPLMVSIHSFISFPVIASLLSELKL 180
           +AWIIG+PS+ +P+ CGL V S+  +GL+ +E   +PLMVS+ S ISFPVIA LL+ELK+
Sbjct: 140 RAWIIGLPSILMPLTCGLLVSSFLLEGLTISEIRKLPLMVSMQSMISFPVIACLLNELKI 199

Query: 181 VSTELGRLGMSSALIADLFSQFVMGTANQIRISRTSITLGYFSKAGIMVQICLILFVLRP 240
           VSTELGRLG+SSAL+AD+FSQ  +  ANQIRISR +   GY+S  G+ VQ+ ++ F+ RP
Sbjct: 200 VSTELGRLGLSSALVADMFSQCAVAIANQIRISRKNAGKGYYSIGGLCVQVFMVSFLFRP 259

Query: 241 SVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLGLAIPDGATQLG 300
           +VLW++KQTPEGKP SRGTT  +FL VL+SAV S LLGQPA+ GPYLLGL++ DG   +G
Sbjct: 260 AVLWIMKQTPEGKPASRGTTQTVFLVVLLSAVTSTLLGQPAVVGPYLLGLSLTDGGP-MG 319

Query: 301 FSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAAD-ARVNIVLSLVTYGVKLVCC 360
           FSL+EKLECFVSDFF+P+FVITC+LQVD+S+I +V    D  RVNI+L+ VTY  +  C 
Sbjct: 320 FSLIEKLECFVSDFFMPVFVITCALQVDLSQILLVAVVDDFTRVNIILAFVTYVTQFFCT 379

Query: 361 FLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFIVFLLIVATFV 420
           FL SL C+L  RDS +LSLIL  KGV EL+FC++ T+Y +I+RGILAWF VFLL++ATFV
Sbjct: 380 FLTSLYCQLSFRDSLMLSLILGSKGVVELSFCTLFTEYNIISRGILAWFTVFLLLIATFV 439

Query: 421 PLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLLNLSCPTPQNP 480
           P+ +K L D  +     QNRNIMHLS NSE RVL C+HKNE+IYG IHLLN+SCPT +NP
Sbjct: 440 PIMLKCLNDISKLQASNQNRNIMHLSQNSEFRVLACVHKNENIYGFIHLLNISCPTLENP 499

Query: 481 LAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNSAGSVYAECFT 540
           +AVYALHLIELVGRTTP+FISHR EN  I DQTYSEN+ LSFDHFEKDNS GSVYAECFT
Sbjct: 500 VAVYALHLIELVGRTTPVFISHRIENKPIGDQTYSENMLLSFDHFEKDNS-GSVYAECFT 559

Query: 541 VITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQKADCSV 600
            I+PHKFM NDICKLA+DK+TSLIILPFH TWT+DGLIDQEDN +RNLNC V++KA CSV
Sbjct: 560 SISPHKFMHNDICKLAMDKITSLIILPFHITWTSDGLIDQEDNTMRNLNCCVIEKAPCSV 619

Query: 601 AIFADKGHLGSVASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELTVLKLG 660
           AI ADKGHLGS+ASMASSG +  Y+VC+IY+GGSDDREAISFAKR+AK+  IELTVLKLG
Sbjct: 620 AILADKGHLGSIASMASSGVKCEYTVCVIYMGGSDDREAISFAKRLAKDIKIELTVLKLG 679

Query: 661 SPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVNEFDLM 720
           S +ED  +SKWE MLDSEV+KDFKM CLG+G V++ EEV +DGPQTAL LR++VN+FDLM
Sbjct: 680 SSVEDNGTSKWEKMLDSEVIKDFKMTCLGDGRVKFLEEVSEDGPQTALRLRELVNDFDLM 739

Query: 721 IVGRRDGLES-PQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 769
           IVGRR G+ES PQT+GLSEWNEFPELG+LGDLIASLD NTRTSVLVIQQQK
Sbjct: 740 IVGRRKGMESSPQTSGLSEWNEFPELGILGDLIASLDINTRTSVLVIQQQK 787

BLAST of MC10g0085 vs. TAIR 10
Match: AT5G22900.1 (cation/H+ exchanger 3 )

HSP 1 Score: 486.9 bits (1252), Expect = 3.0e-137
Identity = 299/804 (37.19%), Postives = 471/804 (58.58%), Query Frame = 0

Query: 4   VQPWRVSSDGIW--GKFSRP----QWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSK 63
           V P   SS+G+W   KFS P     +W N + P L++   +   L   +H  L+  G+ +
Sbjct: 25  VLPINPSSNGVWPQQKFSDPNINVHFW-NYAFPHLQMIFLIISFLWQFLHFFLRRLGMIR 84

Query: 64  ISSQIITGLVFGCSWGQLNNKKLEFFRIN--SEEIIGLLACFGYMLFLFITAVKMDVRRT 123
            +S ++TG++   S+ + N+    FF      E +  L A   YM+F F+  VKMD    
Sbjct: 85  FTSHMLTGVLLSKSFLKENSAARRFFSTEDYKEIVFSLTAACSYMMFWFLMGVKMDTGLI 144

Query: 124 MKTGRKAWIIGIPSVFV-PVICG-LFVGSYFQDGLSSAEK--NTVPLMV--SIHSFISFP 183
             TGRKA  IG+ SV +  ++C  +F G+    G  +++   N++  +V  SI    SFP
Sbjct: 145 RTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSDHTLNSLEYVVIYSIQCLSSFP 204

Query: 184 VIASLLSELKLVSTELGRLGMSSALIADLFSQFVMGTA----NQIRISRT---SITLG-- 243
           V+ +LL EL+L ++ELGRL +SSA+I+D FS  ++ +      +++  +T   S+ +G  
Sbjct: 205 VVGNLLFELRLQNSELGRLAISSAVISD-FSTSILASVLIFMKELKDEQTRLGSVFIGDV 264

Query: 244 -----YFSKAGIMVQ-ICLILFVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVAS 303
                   +AGI+V  +C+ ++V RP + +++KQTP G+PV       I + V  SA+ +
Sbjct: 265 IAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQTPSGRPVKAIYLSTIIVMVSGSAILA 324

Query: 304 ALLGQPAIFGPYLLGLAIPDGATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFI 363
               Q    GP++LGLA+P G   LG ++++K E  +   FLP F+ + S ++DIS +F 
Sbjct: 325 NWCKQSIFMGPFILGLAVPHG-PPLGSAIIQKYESAIFGTFLPFFIASSSTEIDISALF- 384

Query: 364 VLGAADARVNIVLSLVTYGVKLVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSII 423
             G       I++ + ++ VK +   + +L   +P+ D F LSLI+S KG+FEL   ++ 
Sbjct: 385 --GWEGLNGIILIMVTSFVVKFIFTTVPALFYGMPMEDCFALSLIMSFKGIFELGAYALA 444

Query: 424 TQYEVINRGILAWFIVFLLIVATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLP 483
            Q   +         +++ + +  +P  +++L DP R + GY+ RN+ HL PNSELR+L 
Sbjct: 445 YQRGSVRPETFTVACLYITLNSAIIPPILRYLYDPSRMYAGYEKRNMQHLKPNSELRILS 504

Query: 484 CIHKNEHIYGLIHLLNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTYS 543
           CI++ + I  +I+LL   CP+ ++P+A Y LHL+ELVG+  PIFISH+ +     + +YS
Sbjct: 505 CIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQANPIFISHKLQTRRTEETSYS 564

Query: 544 ENVTLSFDHFEKDNSAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTAD 603
            NV +SF+ F KD   GSV+   +T ++    M  DIC LAL+  TSLI+LPFH+TW+AD
Sbjct: 565 NNVLVSFEKFRKD-FYGSVFVSTYTALSMPDTMHGDICMLALNNTTSLILLPFHQTWSAD 624

Query: 604 G-LIDQEDNMLRNLNCSVLQKADCSVAIFADKGHLG----SVASMASSGT---QFRYSVC 663
           G  +   +NM+RNLN SVL  A CSV +F  +   G    S      +GT      Y++C
Sbjct: 625 GSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKNISSGRKTINGTVPNLSSYNIC 684

Query: 664 LIYLGGSDDREAISFAKRMAKEPTIELTVLKLGSPMEDG-SSSKWENMLDSEVLKDFKMN 723
           +I+LGG DDREA++ A RMA++P I +T+++L +  E    ++ W+ MLD E+L+D K N
Sbjct: 685 MIFLGGKDDREAVTLATRMARDPRINITIVRLITTDEKARENTVWDKMLDDELLRDVKSN 744

Query: 724 CLGNGGVRYFEEVCDDGPQTALTLRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELG 770
            L +  + Y E+  +D  +T+  LR +V++FD+ IVGR +G  S  T GL EW+EF ELG
Sbjct: 745 TLVD--IFYSEKAIEDAAETSSLLRSMVSDFDMFIVGRGNGRTSVFTEGLEEWSEFKELG 804

BLAST of MC10g0085 vs. TAIR 10
Match: AT3G44900.1 (cation/H+ exchanger 4 )

HSP 1 Score: 481.5 bits (1238), Expect = 1.3e-135
Identity = 292/797 (36.64%), Postives = 448/797 (56.21%), Query Frame = 0

Query: 4   VQPWRVSSDGIWGKFSRP------QWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSK 63
           + P   SS G+W     P      ++W N   P +++   +  +L    H  L+  G+ +
Sbjct: 24  ILPINPSSSGLWPSPKLPDPQANIEFW-NYMFPHVQIIFLIVTILWQFFHFFLRRLGMIR 83

Query: 64  ISSQIITGLVFGCSWGQLNNKKLEFFRIN--SEEIIGLLACFGYMLFLFITAVKMDVRRT 123
            +S ++TG++   S+ + N    +F       E + GL+    YM+F F+  VKMD+   
Sbjct: 84  FTSHMLTGILLSKSFLKENTPARKFLSTEDYKETLFGLVGACSYMMFWFLMGVKMDLSLI 143

Query: 124 MKTGRKAWIIGIPSVFVPV-ICGLFVGSYFQD-GLSSAEK----NTVPLMVSIHSFISFP 183
             TGRKA  IG+ SV + + +C L      +D G    E       +  +  I    SFP
Sbjct: 144 RSTGRKAVAIGLSSVLLSITVCALIFFLILRDVGTKKGEPVMSFFEIIFIYLIQCLSSFP 203

Query: 184 VIASLLSELKLVSTELGRLGMSSALIADLFSQF---VMGTANQIRISRT---SITLG--- 243
           VI +LL EL+L ++ELGRL MSSA+I+D  +     V+    +++  ++   S+ +G   
Sbjct: 204 VIGNLLFELRLQNSELGRLAMSSAVISDFSTSILSAVLVFLKELKDDKSRLGSVFIGDVI 263

Query: 244 ----YFSKAG-IMVQICLILFVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASA 303
                  +AG +++ +C  +++ RP + +++K+TP G+PV +   + I + V  SA+ + 
Sbjct: 264 VGNRPMKRAGTVVLFVCFAIYIFRPLMFFIIKRTPSGRPVKKFYIYAIIILVFGSAILAD 323

Query: 304 LLGQPAIFGPYLLGLAIPDGATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIV 363
              Q    GP++LGLA+P G   LG ++++K E  V   FLP FV T + ++D S   I+
Sbjct: 324 WCKQSIFIGPFILGLAVPHG-PPLGSAILQKFESVVFGTFLPFFVATSAEEIDTS---IL 383

Query: 364 LGAADARVNIVLSLVTYGVKLVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIIT 423
               D +  ++L  V++ VK     L +    +P +D   LSLI+S KG+FE        
Sbjct: 384 QSWIDLKSIVILVSVSFIVKFALTTLPAFLYGMPAKDCIALSLIMSFKGIFEFGAYGYAY 443

Query: 424 QYEVINRGILAWFIVFLLIVATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPC 483
           Q   I         +++L+ +  +P  +K + DP R + GY+ RN++H+ PNSELR+L C
Sbjct: 444 QRGTIRPVTFTVLSLYILLNSAVIPPLLKRIYDPSRMYAGYEKRNMLHMKPNSELRILSC 503

Query: 484 IHKNEHIYGLIHLLNLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTY-S 543
           I+K + I  +I+LL  +CP+ +NP+A Y LHL+ELVG+  P+ ISHR +     + +Y S
Sbjct: 504 IYKTDDIRPMINLLEATCPSRENPVATYVLHLMELVGQANPVLISHRLQTRKSENMSYNS 563

Query: 544 ENVTLSFDHFEKDNSAGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTAD 603
           ENV +SF+ F  D   GSV+   +T ++  K M  DIC LAL+  TSLIILPFH+TW+AD
Sbjct: 564 ENVVVSFEQFHND-FFGSVFVSTYTALSVPKMMHGDICMLALNNTTSLIILPFHQTWSAD 623

Query: 604 G-LIDQEDNMLRNLNCSVLQKADCSVAIFADKGHLGSVASMASSGTQFRYSVCLIYLGGS 663
           G  I  +  M+R LN SVL  + CSV IF  +   G      ++     Y VC+++LGG 
Sbjct: 624 GSAIVSDSLMIRQLNKSVLDLSPCSVGIFVYRSSNGRRTIKETAANFSSYQVCMLFLGGK 683

Query: 664 DDREAISFAKRMAKEPTIELTVLKL-GSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGV 723
           DDREA+S AKRMA++  I +TV+ L  S      ++ W+ MLD E+L+D K N L    +
Sbjct: 684 DDREALSLAKRMARDSRITITVVSLISSEQRANQATDWDRMLDLELLRDVKSNVLAGADI 743

Query: 724 RYFEEVCDDGPQTALTLRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIA 770
            + EEV +D  QT+  L+ I NE+DL IVGR  G +S  T GL EW+EF ELG++GDL+ 
Sbjct: 744 VFSEEVVNDANQTSQLLKSIANEYDLFIVGREKGRKSVFTEGLEEWSEFEELGIIGDLLT 803

BLAST of MC10g0085 vs. TAIR 10
Match: AT3G44910.1 (cation/H+ exchanger 12 )

HSP 1 Score: 438.7 bits (1127), Expect = 9.5e-123
Identity = 277/781 (35.47%), Postives = 424/781 (54.29%), Query Frame = 0

Query: 7   WRVSSDGIWGKFSRPQWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSKISSQIITGL 66
           + +SS G W     P      SLPL+E Q+ L F+ + IIH  LK FG+S I S ++ GL
Sbjct: 15  FNISSFGFWENLKSPDVIFGYSLPLMEFQILLIFVFIIIIHSFLKSFGISPIPSYMLAGL 74

Query: 67  VFG---CSWGQLNNKKLEFFRI--NSEEIIGLLACFGYMLFLFITAVKMDVRRTMKTGRK 126
           + G    +  +++++KL +      +  + GL  C   ML  F+T VK+  R     G  
Sbjct: 75  ILGPQLFNLREVSSRKLSWDPALDGNGPLRGLSVCGNIMLAFFMT-VKISRRLAFNNGWL 134

Query: 127 AWIIGIPSVFVPVICGLFVGSYFQDGLSS--AEKNTV----PLMVSIHSFISFPVIASLL 186
             +IG  S  VP + G  V +   D +       N V     +++S  S I  P +   L
Sbjct: 135 PIVIGTLSFIVPFLGGFCVRNLHTDNIDPYYMSPNKVLAERIVVISSQSSILLPTVVHFL 194

Query: 187 SELKLVSTELGRLGMSSALIADLFSQ------FVMGTANQIRISRTSITLGYFSKAGIMV 246
           SELK++++ELGRL +S++LI D+F+       +++GT   I     S    Y     +++
Sbjct: 195 SELKILNSELGRLVLSASLINDIFASTVSIFAYLVGTYKNI-----SPMTAYRDLIAVII 254

Query: 247 QICLILFVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLG 306
            I +   VLRP V W++++TPEGKPV+    H + L V+ SA  S+      + GP+LLG
Sbjct: 255 LILVAFCVLRPVVEWIVERTPEGKPVADVYVHAVVLSVIASAAYSSFFNMKYLLGPFLLG 314

Query: 307 LAIPDGATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAADARVNIVLSL 366
           + IP+G   +G +L  K E    +  +P+ +   +++ D+ K  IV    D   NI L  
Sbjct: 315 IIIPEG-PPIGSALEAKYEALTMNVLIPISITFSTMRCDVMK--IVYQYDDIWYNIFLMT 374

Query: 367 VTYGVKLVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFI 426
            T  +K+    +  L CK+P +++   SL+L  K   E+           I++    + I
Sbjct: 375 FTGFLKMATGMVPCLYCKIPFKEAIAASLLLCSKSFSEIFLYESTYDDSYISQATYTFLI 434

Query: 427 VFLLIVATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLL 486
              LI +  +P  +  L DPKR + GYQ +NIM+L P+S+LR+L CIH+ E+I   I  L
Sbjct: 435 TCALINSGIIPTALAGLYDPKRKYVGYQKKNIMNLKPDSDLRILTCIHRPENISAAISFL 494

Query: 487 NLSCPTPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNS 546
                T    + V  LHL++LVG+T P+ ISH ++ N +   +Y     L+F   E    
Sbjct: 495 QFLPST----IVVTVLHLVKLVGKTVPVLISHNKQINRVVTNSYIHTANLAFSQLE---- 554

Query: 547 AGSVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNC 606
             SV    FT IT    M ++ICK+AL++ TS+II+P  R WT DG  + ED  +R LN 
Sbjct: 555 --SVTMTMFTAITHENLMHDEICKVALEQATSIIIVPSGRKWTVDGAFESEDEAIRRLNE 614

Query: 607 SVLQKADCSVAIFADKGHLGSVASMASSGT-QFRYSVCLIYLGGSDDREAISFAKRMAKE 666
           S+L+ A CS+ I  D+G L      +  GT +F   V +I++GG DDREA+S  K+M + 
Sbjct: 615 SLLKSASCSIGILVDRGQL------SLKGTRKFNIDVGVIFIGGKDDREALSLVKKMKQN 674

Query: 667 PTIELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALT 726
           P +++TV++L S  E   S+ W+ +LD EVL+D K +      + Y E +   GP+ A T
Sbjct: 675 PRVKITVIRLISDRET-ESTNWDYILDHEVLEDLK-DTEATNSIAYTERIVTGGPEVATT 734

Query: 727 LRKIVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQ 770
           +R +  ++DLM+VGR  G+ SP   GL EW E PELGV+GDL+AS + ++R SVLV+QQQ
Sbjct: 735 VRSLSEDYDLMVVGRDHGMASPDFDGLMEWVELPELGVIGDLLASRELDSRVSVLVVQQQ 768

BLAST of MC10g0085 vs. TAIR 10
Match: AT3G44920.1 (cation/H+ exchanger 11 )

HSP 1 Score: 425.2 bits (1092), Expect = 1.1e-118
Identity = 265/775 (34.19%), Postives = 414/775 (53.42%), Query Frame = 0

Query: 7   WRVSSDGIWGKFSRPQWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSKISSQIITGL 66
           + +SS G W     P      SLPLLE+Q+ L F  + + H+ L+  GVS+I S +I GL
Sbjct: 15  FNISSQGFWENLKSPDVVFGYSLPLLEIQIILIFFCIVMSHMFLRCIGVSQIVSYMIAGL 74

Query: 67  VFGCSWGQLNNK---KLEFF-RINSEEIIGLLACFGYMLFLFITAVKMDVRRTMKTGRKA 126
           + G     +  K   KL     ++    +  ++ FG ++F F+  V+   R    +G+  
Sbjct: 75  ILGPQLFDILEKSSGKLSADPALDGTAALRCISVFGRLMFTFLMTVRTSRRVAFHSGKLP 134

Query: 127 WIIGIPSVFVPVICGLFVGSYFQDGLS----SAEKNTV--PLMVSIHSFISFPVIASLLS 186
            +IGI S F P+    F+ + F D +     S +K      ++V   S I  P    +L 
Sbjct: 135 VVIGIVSFFAPLFSLSFL-NLFTDNIDPHYMSLDKALAERTVIVITQSQILLPSTTYILL 194

Query: 187 ELKLVSTELGRLGMSSALIADLFSQFVMGTA-NQIRISRTSITLGYFSKAGIMVQICLIL 246
           ELK++++ELGRL +S++ I D+   F M  A  Q      S  + Y     +++   ++ 
Sbjct: 195 ELKIINSELGRLALSASAINDMLGIFAMIVATTQATYIHVSHAIAYRDLVAVIIFFLIVF 254

Query: 247 FVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLGLAIPDG 306
           FV +P V W++ +TPE KPV     H + L    SA          + GP ++G+ IP+G
Sbjct: 255 FVFKPMVQWIIDRTPEDKPVEDIYIHAVILTAFASAAYFVFFNMKYVLGPLIIGIIIPEG 314

Query: 307 ATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAADARVNIVLSLVTYGVK 366
              LG +L  K E    + FLP+ +   +++ D   + I+    D   NI L+L+   +K
Sbjct: 315 -PPLGSALEAKFERLTMNVFLPISITFSAMRCD--GLRILSQFTDIYFNIFLTLLILVIK 374

Query: 367 LVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFIVFLLIV 426
           LV C    L  KLP  +S  +SLILS K   E      + + + I++   A+ I++ L+ 
Sbjct: 375 LVACLTLCLYYKLPRSESLAVSLILSYKSFVEFVLYEAVLEEKFISQATYAFLILYSLLS 434

Query: 427 ATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLLNL-SCP 486
           A  VP+ V+ + DPKR +  YQ R+I+HL  NS LR+L C+HK E++   I  L L S P
Sbjct: 435 AGIVPMVVRSMYDPKRKYVNYQKRDILHLEANSGLRILTCLHKPENVSETIAFLQLFSSP 494

Query: 487 TPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNSAGSVY 546
               P+AV  LHL++LVG+  PI +SH ++   +   +Y     L+F  F ++ S  SV 
Sbjct: 495 IHDFPIAVTVLHLVKLVGQINPIIVSHDKKLKRLHKNSYIHTANLAFRQFMQE-SLESVT 554

Query: 547 AECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSVLQK 606
              FT  +    M  DIC LALD+ TS+I++P  R WT DG+ + +D   R LN S+L +
Sbjct: 555 VTTFTAFSHENLMHEDICTLALDRTTSMIVVPSGRKWTVDGMFESDDLAARQLNQSLLDR 614

Query: 607 ADCSVAIFADKGHLGSVASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTIELT 666
           A CS+ I  D+G   S  S  +S  ++   V ++++GG DDREA+S  KRM   P + +T
Sbjct: 615 APCSIGILVDRGQF-SRKSYVTSKNRYNIDVGVLFIGGKDDREALSLVKRMKYNPRVRVT 674

Query: 667 VLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRKIVN 726
           V++L    +    S+W+ +LD+E LKD K +   N  + Y E +     +    ++ +  
Sbjct: 675 VIRL--IFDHEIESEWDYILDNEGLKDLK-STESNEDILYTERIVTSVVEVVKAVQLLAE 734

Query: 727 EFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 770
           E+DLM+VGR   + S   +GL+EW E PELGV+GDL+A+ D N++ SVLV+QQQ+
Sbjct: 735 EYDLMVVGRDHDMTSQDLSGLTEWVELPELGVIGDLLAARDLNSKVSVLVVQQQQ 780

BLAST of MC10g0085 vs. TAIR 10
Match: AT3G44930.1 (cation/H+ exchanger 10 )

HSP 1 Score: 420.6 bits (1080), Expect = 2.7e-117
Identity = 262/778 (33.68%), Postives = 415/778 (53.34%), Query Frame = 0

Query: 7   WRVSSDGIWGKFSRPQWWLNNSLPLLELQLFLFFLLMSIIHLLLKPFGVSKISSQIITGL 66
           + +SS G W     P      SLPLLE+Q+ L F  + + H+ L+  G+S+I+S +I G+
Sbjct: 15  FNISSQGFWDNLKSPDVVFGYSLPLLEIQIILIFFCIVMSHMFLRCIGISQIASYMIAGI 74

Query: 67  VFGCSWGQLNNKKLEFFRIN-SEEIIGLLAC---FGYMLFLFITAVKMDVRRTMKTGRKA 126
           V G     +  K      ++ + + I  L C   FG ++F F+  V+   R    +G+  
Sbjct: 75  VLGPQLFDVLEKSSGKLSVDPALDGIAALRCISVFGTLMFTFLMTVRTSRRVAFHSGKLP 134

Query: 127 WIIGIPSVFVPVICGLFVGSYFQDGLSSAEKNTVPL---------MVSIHSFISFPVIAS 186
            +IGI S F P+  GL   ++F D +   + + +PL         +V   S I  P    
Sbjct: 135 VVIGIVSFFAPLF-GLGFQNFFSDNI---DPHYMPLTKALGERTAIVITQSSILLPSTTY 194

Query: 187 LLSELKLVSTELGRLGMSSALIADLFSQFVMGTAN-QIRISRTSITLGYFSKAGIMVQIC 246
           +L ELK++++ELGRL +S+ +I D+   F M  A+ Q      S    Y     +++   
Sbjct: 195 ILLELKIINSELGRLALSACVINDILGIFSMIVASIQATYIHVSHATAYRDTVAVIIFFL 254

Query: 247 LILFVLRPSVLWLLKQTPEGKPVSRGTTHGIFLFVLISAVASALLGQPAIFGPYLLGLAI 306
           ++  V +P V W++ +TPE KPV     H + +  L SA          I GP ++G+ I
Sbjct: 255 VVFLVFKPMVQWVIDRTPEDKPVEDMYIHAVIITALASAAYFVFFNMKYILGPLMIGIII 314

Query: 307 PDGATQLGFSLVEKLECFVSDFFLPLFVITCSLQVDISKIFIVLGAADARVNIVLSLVTY 366
           P+G   LG +L  K E    + FLP+ +   +++ D ++I       D   NI L+ +  
Sbjct: 315 PEG-PPLGSALEAKFERLTMNVFLPISITFSAMRCDGARILSQFN--DIFFNIFLTFLIL 374

Query: 367 GVKLVCCFLCSLCCKLPLRDSFVLSLILSCKGVFELAFCSIITQYEVINRGILAWFIVFL 426
            +KLV C    L  KLPL +S  +S ILS K   +      +     I++   ++ I++ 
Sbjct: 375 VIKLVACLAPCLYYKLPLSESLAVSFILSYKSFADFVLYEAVLDDTYISQATYSFLILYS 434

Query: 427 LIVATFVPLTVKWLTDPKRTHDGYQNRNIMHLSPNSELRVLPCIHKNEHIYGLIHLLN-L 486
           L+ A  VP  ++ + DP+R +  YQ R+I+HL  NS+LR+L C+HK E++   I  L  L
Sbjct: 435 LLNAGIVPTVLRRMYDPRRKYVNYQKRDILHLERNSDLRILTCLHKPENVSETIAFLQLL 494

Query: 487 SCPTPQNPLAVYALHLIELVGRTTPIFISHRQENNAISDQTYSENVTLSFDHFEKDNSAG 546
           S P    P+AV  LHL++LVG+  PI +SH ++   ++  +Y     L+F  F  + S  
Sbjct: 495 SSPNLDFPIAVTVLHLVKLVGQINPIIVSHDKKLKRLNKDSYIHTANLAFRQFVLE-SLE 554

Query: 547 SVYAECFTVITPHKFMTNDICKLALDKVTSLIILPFHRTWTADGLIDQEDNMLRNLNCSV 606
           SV    FT  +    M  DIC LALDK TS+I++P  R WT DGL + ++  +R+LN S+
Sbjct: 555 SVTVTTFTAFSHENLMHEDICTLALDKTTSMIVVPSGRKWTVDGLFESDNTAIRHLNQSL 614

Query: 607 LQKADCSVAIFADKGHLGSVASMASSGTQFRYSVCLIYLGGSDDREAISFAKRMAKEPTI 666
           L +A CS+ I  D+G   S  S+ +S  ++   V ++++GG DDREA+S  KRM   P I
Sbjct: 615 LDRAPCSIGILVDRGQF-SRKSIVTSKKRYIIDVGVLFIGGKDDREALSLVKRMKNNPRI 674

Query: 667 ELTVLKLGSPMEDGSSSKWENMLDSEVLKDFKMNCLGNGGVRYFEEVCDDGPQTALTLRK 726
            +TV++L    +    S W+ +LD+E LKD K +   N  + Y E +     +    ++ 
Sbjct: 675 RVTVIRL--VFDHEIESDWDYILDNEGLKDLK-STEDNKDIDYIERIVTSSVEVVKAVQL 734

Query: 727 IVNEFDLMIVGRRDGLESPQTAGLSEWNEFPELGVLGDLIASLDNNTRTSVLVIQQQK 770
           +  E+DLM+VGR   + S   +GL EW E PELGV+GDL+A+ D +++ SVLV+QQQ+
Sbjct: 735 LAEEYDLMVVGRDHDMTSQDLSGLMEWVELPELGVIGDLLAARDLSSKVSVLVVQQQQ 780

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FFB84.3e-13637.19Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1[more]
Q9FYC11.8e-13436.64Cation/H(+) antiporter 4 OS=Arabidopsis thaliana OX=3702 GN=CHX4 PE=2 SV=1[more]
Q9FYC01.3e-12135.47Cation/H(+) antiporter 12 OS=Arabidopsis thaliana OX=3702 GN=CHX12 PE=2 SV=2[more]
Q9FYB91.5e-11734.19Cation/H(+) antiporter 11 OS=Arabidopsis thaliana OX=3702 GN=CHX11 PE=2 SV=2[more]
Q58P693.8e-11633.68Cation/H(+) antiporter 10 OS=Arabidopsis thaliana OX=3702 GN=CHX10 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_022141218.10.087.14cation/H(+) antiporter 3-like [Momordica charantia][more]
XP_022141141.10.099.85cation/H(+) antiporter 4-like [Momordica charantia][more]
KGN57865.10.069.39hypothetical protein Csa_011167 [Cucumis sativus][more]
XP_016899409.10.069.26PREDICTED: cation/H(+) antiporter 4-like [Cucumis melo][more]
TYK03148.10.069.26cation/H(+) antiporter 4-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A6J1CJU70.087.14cation/H(+) antiporter 3-like OS=Momordica charantia OX=3673 GN=LOC111011670 PE=... [more]
A0A6J1CI530.099.85cation/H(+) antiporter 4-like OS=Momordica charantia OX=3673 GN=LOC111011605 PE=... [more]
A0A0A0L7A80.069.39Na_H_Exchanger domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G355... [more]
A0A1S4DTU40.069.26cation/H(+) antiporter 4-like OS=Cucumis melo OX=3656 GN=LOC103485746 PE=4 SV=1[more]
A0A5D3BVZ80.069.26Cation/H(+) antiporter 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
Match NameE-valueIdentityDescription
AT5G22900.13.0e-13737.19cation/H+ exchanger 3 [more]
AT3G44900.11.3e-13536.64cation/H+ exchanger 4 [more]
AT3G44910.19.5e-12335.47cation/H+ exchanger 12 [more]
AT3G44920.11.1e-11834.19cation/H+ exchanger 11 [more]
AT3G44930.12.7e-11733.68cation/H+ exchanger 10 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038770Sodium/solute symporter superfamilyGENE3D1.20.1530.20coord: 32..434
e-value: 5.4E-52
score: 178.9
IPR006153Cation/H+ exchangerPFAMPF00999Na_H_Exchangercoord: 38..426
e-value: 1.1E-31
score: 110.0
NoneNo IPR availablePANTHERPTHR32468CATION/H + ANTIPORTERcoord: 16..769
NoneNo IPR availablePANTHERPTHR32468:SF17CATION/H(+) ANTIPORTER 4coord: 16..769

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0085.1MC10g0085.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 proton transmembrane transport
biological_process GO:0006885 regulation of pH
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0012505 endomembrane system
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015299 solute:proton antiporter activity