MC10g0078 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC10g0078
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionWRKY transcription factor 13
LocationMC10: 498149 .. 500305 (+)
RNA-Seq ExpressionMC10g0078
SyntenyMC10g0078
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCTTTATGTTTTAATTTGGCCAATCTCTGTTTTTAAGTGGAATTGGTCAGCAATTGGACTTTGATCTCTCAATCAATTTACAAACAATGTATCTACGACCGAAGACTGGTCTCCTTTGAAGAGAGTCAAACTCTCTCTCTCCCTCCTTTTCCTTTGGATTCAAAAATATTCAGAGCTTTCTTTTAAATTGCCTCCTTCATCACCTGCTTCCTCTTCACGATTCCCCATCTCTTTTCTCCATTTCTCTACTCGGATTCATATCTTTTGCTCCTCTTTCACAATACCCACTAGTGTAAAACTGTAAAGTCTCTGTTTTTTCAACTTTATCTCACAACCCATTTCATATCTTTGCTTTAATTCTAACATTATATTGTTTCCTCACCTTCCTTTGAAACTTTAATGCTACCTTTCTGGTGTCAGCCTTATATATGCATTTCCTAGCTGTCTAGCATTTGCTCATAGCATTACTCTTCTTCTTCTTCTTCTCCAACTTTGAGGACATAAATTTGGCAGTTGTGATTAAATGGAGAATATTGGGGAATGGGAGCAGAAGAATCTGAAAAATGAGCTACTTAGAGGGAAGGAATTGGCAAAGCAGCTCCAACTTTATCTCAATGTGCAACCCTCATCATCCATGCCTGCTTCTCCTTCTGAAGATGGTGAATTATTAGTTCAGAAAATCTTATCTTCTTATGAAAAGGCACTCTCATTTTTCAGCTCAACTGGGATCCAAATATCTGAATCTCCATCATCCCCTAATGGAAGTCCAAGGAGTGAGGACTCTGACCGTGAATTCAAGGACCAGGACCAGAGAATTAATGCTTCCCGCAAAAGGTAACTTTGAACAAAAGCTTATTTATGATCTGAACTGTTCTTAAAATCTCACAGTTTTGAGATATTTCCTGTTTTGTATGTTTGTAGGAACAGTCTTCCAACTTGGACACAGAAATTTCAAGTTAGCCCAGGGATGGCCCCTGAAGGCTCTCTTGATGATGGCTTTTGCTGGAGGAAATATGGTCAAAAAGACATTCTTGGTGCCAAACATCCAAGGTAAAATCTTTGGATCTCTTAACTATTCTAACATTTATTTGCCTATTTTGCTGTTTTAAAGCATTTTTGCAGCAAAATGGTTATATGGTTATGTGTCTGTTGCACACCAGCAGAGGCTATTACAGATGCACGCACAAGAACCTCCAAGGTTGTCTTGCAACCAAACAAGTTCAACGCTCCGACGATGATCCGACCGTCTTCGAAATCACGTACCGAGGAAACCATACTTGCAGCCAAGTCACCAACCTCAGTCCTCCATCAGCAGCACCCGAGTTTCAGCAACCAAACAGTAGCAATATTGATCCAAATTTGGTGCAAAAGCAGAGCCACTCTGTTCCTGACCAAAACCAGCAAAGTTCACCTGATGCTCTCTTGAACTCTTGGGCATCCCTCAGAGTCATAACTGAAAATCTTGACACTCATGAACCAACATTGTTTCCCGCTTTTAGTTATGATCCCACATCAAGTTACAAGGCTGCAGACCATGTCGAGTCGGCATCGATGACGGTTGGTGTGAACTTTGCCGAGTTTCCTCCTTCATTTTTATCCCCAACAACATCTTGTTCTGGGTTGAGCTATTTCTCTGCCTCCTCAGGTGGGTTGAGTGAAGGATTTGTTGGGAATCAGAAGTTGCAGGCAAACAAATCTGAGCTTTCTTTTCCCTCACCAACTTCAGCTCTAAACTCTCAAACTCTTGGCTCAGAGTTCCCATTTGGTGAGGTTGAAATGGAGCCAAGCTTCACATTTGACCACACAAATTTCTTCTCCTAATTCTCCTCGGTTCTAAACTCGAACACTCGCAATCATACTGTGAAGCCTTTTTTTTTTTTTTTTTTTTTGTGTAGTAAAGTAACGTTGAAGCCCAAAATGTTAATTCTTACCATCTTTGAAGATGTTCAAGTTTGTATTTCTAGAGAAATTGTAACAGTAATTCATGTTTGAGAATATTATAACATTGTAGTTAACTTTGTTAACTATGAATTTTATAAGAGAATTATTTAAATTAGTACTAAAGCCTAAAGTACAATTCGAATCATGGAGGCCCACTTGAGCATTAAGCCCATTGTTTGGGCCATAACAAGGACCAAGCCCACACATAAAAATT

mRNA sequence

CTCCTTTATGTTTTAATTTGGCCAATCTCTGTTTTTAAGTGGAATTGGTCAGCAATTGGACTTTGATCTCTCAATCAATTTACAAACAATGTATCTACGACCGAAGACTGGTCTCCTTTGAAGAGAGTCAAACTCTCTCTCTCCCTCCTTTTCCTTTGGATTCAAAAATATTCAGAGCTTTCTTTTAAATTGCCTCCTTCATCACCTGCTTCCTCTTCACGATTCCCCATCTCTTTTCTCCATTTCTCTACTCGGATTCATATCTTTTGCTCCTCTTTCACAATACCCACTAGTGTAAAACTGTAAAGTCTCTGTTTTTTCAACTTTATCTCACAACCCATTTCATATCTTTGCTTTAATTCTAACATTATATTGTTTCCTCACCTTCCTTTGAAACTTTAATGCTACCTTTCTGGTGTCAGCCTTATATATGCATTTCCTAGCTGTCTAGCATTTGCTCATAGCATTACTCTTCTTCTTCTTCTTCTCCAACTTTGAGGACATAAATTTGGCAGTTGTGATTAAATGGAGAATATTGGGGAATGGGAGCAGAAGAATCTGAAAAATGAGCTACTTAGAGGGAAGGAATTGGCAAAGCAGCTCCAACTTTATCTCAATGTGCAACCCTCATCATCCATGCCTGCTTCTCCTTCTGAAGATGGTGAATTATTAGTTCAGAAAATCTTATCTTCTTATGAAAAGGCACTCTCATTTTTCAGCTCAACTGGGATCCAAATATCTGAATCTCCATCATCCCCTAATGGAAGTCCAAGGAGTGAGGACTCTGACCGTGAATTCAAGGACCAGGACCAGAGAATTAATGCTTCCCGCAAAAGGAACAGTCTTCCAACTTGGACACAGAAATTTCAAGTTAGCCCAGGGATGGCCCCTGAAGGCTCTCTTGATGATGGCTTTTGCTGGAGGAAATATGGTCAAAAAGACATTCTTGGTGCCAAACATCCAAGAGGCTATTACAGATGCACGCACAAGAACCTCCAAGGTTGTCTTGCAACCAAACAAGTTCAACGCTCCGACGATGATCCGACCGTCTTCGAAATCACGTACCGAGGAAACCATACTTGCAGCCAAGTCACCAACCTCAGTCCTCCATCAGCAGCACCCGAGTTTCAGCAACCAAACAGTAGCAATATTGATCCAAATTTGGTGCAAAAGCAGAGCCACTCTGTTCCTGACCAAAACCAGCAAAGTTCACCTGATGCTCTCTTGAACTCTTGGGCATCCCTCAGAGTCATAACTGAAAATCTTGACACTCATGAACCAACATTGTTTCCCGCTTTTAGTTATGATCCCACATCAAGTTACAAGGCTGCAGACCATGTCGAGTCGGCATCGATGACGGTTGGTGTGAACTTTGCCGAGTTTCCTCCTTCATTTTTATCCCCAACAACATCTTGTTCTGGGTTGAGCTATTTCTCTGCCTCCTCAGGTGGGTTGAGTGAAGGATTTGTTGGGAATCAGAAGTTGCAGGCAAACAAATCTGAGCTTTCTTTTCCCTCACCAACTTCAGCTCTAAACTCTCAAACTCTTGGCTCAGAGTTCCCATTTGGTGAGGTTGAAATGGAGCCAAGCTTCACATTTGACCACACAAATTTCTTCTCCTAATTCTCCTCGGTTCTAAACTCGAACACTCGCAATCATACTGTGAAGCCTTTTTTTTTTTTTTTTTTTTTGTGTAGTAAAGTAACGTTGAAGCCCAAAATGTTAATTCTTACCATCTTTGAAGATGTTCAAGTTTGTATTTCTAGAGAAATTGTAACAGTAATTCATGTTTGAGAATATTATAACATTGTAGTTAACTTTGTTAACTATGAATTTTATAAGAGAATTATTTAAATTAGTACTAAAGCCTAAAGTACAATTCGAATCATGGAGGCCCACTTGAGCATTAAGCCCATTGTTTGGGCCATAACAAGGACCAAGCCCACACATAAAAATT

Coding sequence (CDS)

ATGGAGAATATTGGGGAATGGGAGCAGAAGAATCTGAAAAATGAGCTACTTAGAGGGAAGGAATTGGCAAAGCAGCTCCAACTTTATCTCAATGTGCAACCCTCATCATCCATGCCTGCTTCTCCTTCTGAAGATGGTGAATTATTAGTTCAGAAAATCTTATCTTCTTATGAAAAGGCACTCTCATTTTTCAGCTCAACTGGGATCCAAATATCTGAATCTCCATCATCCCCTAATGGAAGTCCAAGGAGTGAGGACTCTGACCGTGAATTCAAGGACCAGGACCAGAGAATTAATGCTTCCCGCAAAAGGAACAGTCTTCCAACTTGGACACAGAAATTTCAAGTTAGCCCAGGGATGGCCCCTGAAGGCTCTCTTGATGATGGCTTTTGCTGGAGGAAATATGGTCAAAAAGACATTCTTGGTGCCAAACATCCAAGAGGCTATTACAGATGCACGCACAAGAACCTCCAAGGTTGTCTTGCAACCAAACAAGTTCAACGCTCCGACGATGATCCGACCGTCTTCGAAATCACGTACCGAGGAAACCATACTTGCAGCCAAGTCACCAACCTCAGTCCTCCATCAGCAGCACCCGAGTTTCAGCAACCAAACAGTAGCAATATTGATCCAAATTTGGTGCAAAAGCAGAGCCACTCTGTTCCTGACCAAAACCAGCAAAGTTCACCTGATGCTCTCTTGAACTCTTGGGCATCCCTCAGAGTCATAACTGAAAATCTTGACACTCATGAACCAACATTGTTTCCCGCTTTTAGTTATGATCCCACATCAAGTTACAAGGCTGCAGACCATGTCGAGTCGGCATCGATGACGGTTGGTGTGAACTTTGCCGAGTTTCCTCCTTCATTTTTATCCCCAACAACATCTTGTTCTGGGTTGAGCTATTTCTCTGCCTCCTCAGGTGGGTTGAGTGAAGGATTTGTTGGGAATCAGAAGTTGCAGGCAAACAAATCTGAGCTTTCTTTTCCCTCACCAACTTCAGCTCTAAACTCTCAAACTCTTGGCTCAGAGTTCCCATTTGGTGAGGTTGAAATGGAGCCAAGCTTCACATTTGACCACACAAATTTCTTCTCCTAA

Protein sequence

MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKALSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITYRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWASLRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSGLSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFDHTNFFS
Homology
BLAST of MC10g0078 vs. ExPASy Swiss-Prot
Match: Q9SUP6 (Probable WRKY transcription factor 53 OS=Arabidopsis thaliana OX=3702 GN=WRKY53 PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.3e-47
Identity = 128/304 (42.11%), Postives = 169/304 (55.59%), Query Frame = 0

Query: 7   WEQKNLKNELLRGKELAKQLQLYLNVQPS-----SSMPASPSEDGELLVQKILSSYEKA- 66
           WEQK L +EL+ G + AK+LQ  L   PS     SS   + +E  E+LV++I+SSYE++ 
Sbjct: 9   WEQKTLLSELINGFDAAKKLQARLREAPSPSSSFSSPATAVAETNEILVKQIVSSYERSL 68

Query: 67  --LSFFSSTGIQ----------------ISESPSSPNGSPRSED------SDREFKDQDQ 126
             L++ SS  +Q                + ESP+S NGSPRSE+      S      QD 
Sbjct: 69  LLLNWSSSPSVQLIPTPVTVVPVANPGSVPESPASINGSPRSEEFADGGGSSESHHRQDY 128

Query: 127 RINASRKRNSLPTWTQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKN 186
             N S+KR  LP W++K ++SP    EG  DD F WRKYGQKDILGAK PR YYRCTH++
Sbjct: 129 IFN-SKKRKMLPKWSEKVRISPERGLEGPQDDVFSWRKYGQKDILGAKFPRSYYRCTHRS 188

Query: 187 LQGCLATKQVQRSDDDPTVFEITYRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQK 246
            Q C ATKQVQRSD D TVFE+TYRG HTCSQ    +PP A+PE +Q   + + P + QK
Sbjct: 189 TQNCWATKQVQRSDGDATVFEVTYRGTHTCSQAITRTPPLASPEKRQ--DTRVKPAITQK 248

Query: 247 QSHSVPDQNQQSSPDALLNSWASLRVITENLDTHE-----PTLFPAFSYDPTSSYKAADH 276
                         D L +  ++L V T+ LD  +     P   P ++Y   +      H
Sbjct: 249 PK------------DILESLKSNLTVRTDGLDDGKDVFSFPDTPPFYNYGTING--EFGH 295

BLAST of MC10g0078 vs. ExPASy Swiss-Prot
Match: Q8H0Y8 (Probable WRKY transcription factor 41 OS=Arabidopsis thaliana OX=3702 GN=WRKY41 PE=1 SV=2)

HSP 1 Score: 163.7 bits (413), Expect = 3.9e-39
Identity = 93/200 (46.50%), Postives = 123/200 (61.50%), Query Frame = 0

Query: 7   WEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKALSFFSS 66
           WE+++L NEL+ G + AKQLQ   +   S+S     +E  E L+  I+SS++KA+   + 
Sbjct: 6   WERRSLLNELIHGLKAAKQLQGSSSPSLSASSSYLTTEIKENLLHNIVSSFKKAILMLNG 65

Query: 67  TGIQ-----------------ISESPSSPNGSPRSEDSDREFKDQDQRIN-ASRKRNSLP 126
           +  Q                 +  SP+S  G+PRSE+    F  + +  N +S+KR  LP
Sbjct: 66  STTQHNPTIELAPDPLAHPGKVPGSPASITGNPRSEEF---FNVRSKEFNLSSKKRKMLP 125

Query: 127 TWTQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQR 186
            WT++ ++SP    EG  DD F WRKYGQKDILGAK PR YYRCT +N Q C ATKQVQR
Sbjct: 126 KWTEQVRISPERGLEGPHDDIFSWRKYGQKDILGAKFPRSYYRCTFRNTQYCWATKQVQR 185

Query: 187 SDDDPTVFEITYRGNHTCSQ 189
           SD DPT+FE+TYRG HTCSQ
Sbjct: 186 SDGDPTIFEVTYRGTHTCSQ 202

BLAST of MC10g0078 vs. ExPASy Swiss-Prot
Match: Q9FL62 (Probable WRKY transcription factor 30 OS=Arabidopsis thaliana OX=3702 GN=WRKY30 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 5.3e-36
Identity = 113/306 (36.93%), Postives = 164/306 (53.59%), Query Frame = 0

Query: 5   GEWEQ-KNLKNEL-LRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKALS 64
           GEWE+ KN  NEL + G++ A Q              AS  E  E L +KIL SY K+L+
Sbjct: 8   GEWEKMKNEINELMIEGRDYAHQFG-----------SASSQETREHLAKKILQSYHKSLT 67

Query: 65  FFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGMAP 124
             + +G    +  S   GSP+S+DSD+E       I +S+K  S+P W+ K +++PG   
Sbjct: 68  IMNYSG--ELDQVSQGGGSPKSDDSDQE----PLVIKSSKK--SMPRWSSKVRIAPGAGV 127

Query: 125 EGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITYRG 184
           + +LDDGF WRKYGQKDILGAK PRGYYRCT++  QGC ATKQVQRSD++  + EI+YRG
Sbjct: 128 DRTLDDGFSWRKYGQKDILGAKFPRGYYRCTYRKSQGCEATKQVQRSDENQMLLEISYRG 187

Query: 185 NHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQ------------SSP 244
            H+CSQ  N+          +PN +    NL      SV + N Q            S+P
Sbjct: 188 IHSCSQAANVGTTMPIQNL-EPNQTQEHGNL-DMVKESVDNYNHQAHLHHNLHYPLSSTP 247

Query: 245 DALLNSWASLRVITENLDTHEPTLF-----PAFSYDPTSSYKAADHVESASMTVGVN--F 290
           +   N+   L++  +N++    T F      + +Y+  +S  A+    ++  TV +   F
Sbjct: 248 NLENNNAYMLQMRDQNIEYFGSTSFSSDLGTSINYNFPASGSASHSASNSPSTVPLESPF 292

BLAST of MC10g0078 vs. ExPASy Swiss-Prot
Match: Q9SKD9 (Probable WRKY transcription factor 46 OS=Arabidopsis thaliana OX=3702 GN=WRKY46 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 5.9e-27
Identity = 103/295 (34.92%), Postives = 156/295 (52.88%), Query Frame = 0

Query: 8   EQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKALSFFSST 67
           E+K + NEL  GKELA +L    N++ +SS+     +  + L+  IL  Y+ A+   S  
Sbjct: 4   EEKLVINELELGKELANRLM--NNLKHTSSV-----DSNKTLISDILRIYQNAIFMLSFN 63

Query: 68  GIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGMAPE-GSL 127
                      N   RS + D     +D + N  +KR      T+K +V      E GS+
Sbjct: 64  --------QDKNILKRSLEID----GKDSK-NVFKKRKVSEKNTEKVKVFVATEQENGSI 123

Query: 128 DDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITYRGNHTC 187
           DDG CWRKYGQK+I G+K+PR YYRCTH+  Q CLA KQVQ+SD DP++FE+ Y GNHTC
Sbjct: 124 DDGHCWRKYGQKEIHGSKNPRAYYRCTHRFTQDCLAVKQVQKSDTDPSLFEVKYLGNHTC 183

Query: 188 SQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWASLRVITEN 247
           + +T+   P     F   + S  + N+ +     V +Q++   P        SL    E+
Sbjct: 184 NNITS---PKTTTNF---SVSLTNTNIFEGNRVHVTEQSEDMKPTKSEEVMISL----ED 243

Query: 248 LDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSGLS 302
           L+ ++  +F  FS+   S+++  + V  +++ +G NF E     LSP TS S ++
Sbjct: 244 LE-NKKNIFRTFSF---SNHEIENGVWKSNLFLG-NFVE----DLSPATSGSAIT 259

BLAST of MC10g0078 vs. ExPASy Swiss-Prot
Match: Q0DFT7 (Transcription factor WRKY19 OS=Oryza sativa subsp. japonica OX=39947 GN=WRKY19 PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 2.1e-24
Identity = 56/113 (49.56%), Postives = 74/113 (65.49%), Query Frame = 0

Query: 100 ASRKRNSLPTWTQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQG 159
           A +KR  +    ++ +V+  +    SLDDG  WRKYGQKDILGAK+PR Y+RCTH++ QG
Sbjct: 80  AFKKRKGMAKVRRQVRVT-SVQDTASLDDGLSWRKYGQKDILGAKYPRAYFRCTHRHTQG 139

Query: 160 CLATKQVQRSDDDPTVFEITYRGNHTCSQ-----VTNLSPPSAAPEFQQPNSS 208
           C ATKQVQR+D DP +F++ Y G+HTC Q         +PP  A + QQ  SS
Sbjct: 140 CNATKQVQRADGDPLLFDVVYLGDHTCGQAAVAAAAQSAPPEHAGQEQQRQSS 191

BLAST of MC10g0078 vs. NCBI nr
Match: XP_022140884.1 (probable WRKY transcription factor 53 isoform X2 [Momordica charantia])

HSP 1 Score: 724 bits (1870), Expect = 1.98e-263
Identity = 365/365 (100.00%), Postives = 365/365 (100.00%), Query Frame = 0

Query: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60
           MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA
Sbjct: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60

Query: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120
           LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM
Sbjct: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120

Query: 121 APEGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITY 180
           APEGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITY
Sbjct: 121 APEGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITY 180

Query: 181 RGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWASL 240
           RGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWASL
Sbjct: 181 RGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWASL 240

Query: 241 RVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSGL 300
           RVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSGL
Sbjct: 241 RVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSGL 300

Query: 301 SYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFDH 360
           SYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFDH
Sbjct: 301 SYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFDH 360

Query: 361 TNFFS 365
           TNFFS
Sbjct: 361 TNFFS 365

BLAST of MC10g0078 vs. NCBI nr
Match: XP_022140883.1 (probable WRKY transcription factor 53 isoform X1 [Momordica charantia])

HSP 1 Score: 720 bits (1858), Expect = 1.39e-261
Identity = 365/366 (99.73%), Postives = 365/366 (99.73%), Query Frame = 0

Query: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60
           MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA
Sbjct: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60

Query: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120
           LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM
Sbjct: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120

Query: 121 APEGSLDDGFCWRKYGQKDILGAKHP-RGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180
           APEGSLDDGFCWRKYGQKDILGAKHP RGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT
Sbjct: 121 APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180

Query: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240
           YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS
Sbjct: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240

Query: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300
           LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG
Sbjct: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300

Query: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360
           LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD
Sbjct: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360

Query: 361 HTNFFS 365
           HTNFFS
Sbjct: 361 HTNFFS 366

BLAST of MC10g0078 vs. NCBI nr
Match: XP_022140885.1 (probable WRKY transcription factor 53 isoform X3 [Momordica charantia])

HSP 1 Score: 711 bits (1834), Expect = 5.86e-258
Identity = 363/366 (99.18%), Postives = 363/366 (99.18%), Query Frame = 0

Query: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60
           MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA
Sbjct: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60

Query: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120
           LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRK  SLPTWTQKFQVSPGM
Sbjct: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRK--SLPTWTQKFQVSPGM 120

Query: 121 APEGSLDDGFCWRKYGQKDILGAKHP-RGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180
           APEGSLDDGFCWRKYGQKDILGAKHP RGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT
Sbjct: 121 APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180

Query: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240
           YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS
Sbjct: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240

Query: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300
           LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG
Sbjct: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300

Query: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360
           LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD
Sbjct: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360

Query: 361 HTNFFS 365
           HTNFFS
Sbjct: 361 HTNFFS 364

BLAST of MC10g0078 vs. NCBI nr
Match: AZU90763.1 (WRKY transcription factor 13 [Siraitia grosvenorii] >QEE82235.1 WRKY transcription factor 13 [Siraitia grosvenorii])

HSP 1 Score: 520 bits (1338), Expect = 2.54e-182
Identity = 303/383 (79.11%), Postives = 324/383 (84.60%), Query Frame = 0

Query: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSED---------GELLVQ 60
           MENIGEWEQKNLKNELLRGKELA+QLQ+YLNV+ SSSM AS S           GELLV+
Sbjct: 1   MENIGEWEQKNLKNELLRGKELARQLQIYLNVRQSSSMAASSSSSSSSSSSNGGGELLVK 60

Query: 61  KILSSYEKALSFFSS-TGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTW 120
           KILSSYEKALS  SS TGIQISESPS+ NGSPRSEDSD      DQR NASRKRN LPTW
Sbjct: 61  KILSSYEKALSLLSSSTGIQISESPSTLNGSPRSEDSDH-----DQR-NASRKRNILPTW 120

Query: 121 TQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQRSD 180
           TQKFQVSPGMA EGSLDDGFCWRKYGQK ILGAKHPRGYYRCTH+N+QGCLATKQVQR D
Sbjct: 121 TQKFQVSPGMALEGSLDDGFCWRKYGQKGILGAKHPRGYYRCTHRNVQGCLATKQVQRCD 180

Query: 181 DDPTVFEITYRGNHTCSQVTNLSPPSAAPE-FQQPNSSNIDPNLVQKQSHSVPDQNQQSS 240
           DDPTVFEITYRGNHTCSQV+NL+PPSA P+ FQQPNSS ID +LVQ  SH  PDQNQQ+S
Sbjct: 181 DDPTVFEITYRGNHTCSQVSNLTPPSATPDQFQQPNSS-ID-SLVQ--SH-PPDQNQQTS 240

Query: 241 P-DALLNSWASLRVITENLDT-HEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFP 300
           P DALLNSWASL+VITENLDT HEPT FP FSYDPTS+Y+AA+HV+SAS TV VNFAEF 
Sbjct: 241 PPDALLNSWASLKVITENLDTTHEPT-FPPFSYDPTSNYEAANHVKSAS-TVDVNFAEFS 300

Query: 301 PSFLSPTT--SCSGLSYFSASSGGLSEGFVGNQKLQANKSELS--FPSPTSALNSQTLGS 360
           PSF+SPTT  S SGL YF ASS  LSEGFVG+Q LQANK ELS  F SPTSA+NSQTLG 
Sbjct: 301 PSFISPTTTTSGSGLRYFPASSSVLSEGFVGHQNLQANKPELSEIFSSPTSAVNSQTLGL 360

Query: 361 EFPFGEVE-MEPSFTFDHTNFFS 365
           EFPFGEVE MEPSFTF +TNFFS
Sbjct: 361 EFPFGEVELMEPSFTFHNTNFFS 370

BLAST of MC10g0078 vs. NCBI nr
Match: KAG6576671.1 (putative WRKY transcription factor 53, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 474 bits (1220), Expect = 2.23e-164
Identity = 270/385 (70.13%), Postives = 302/385 (78.44%), Query Frame = 0

Query: 1   MENIGEWEQKN-LKNELLRGKELAKQLQLYLNVQPSSSMPA-------SPSEDG-ELLVQ 60
           M++ GEW+QK  LKNELL+GKELAKQLQ++LNV+PS+S  A       S S DG ELLVQ
Sbjct: 1   MDSFGEWDQKKKLKNELLKGKELAKQLQIHLNVRPSTSSMAAAAASSSSASNDGSELLVQ 60

Query: 61  KILSSYEKALSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWT 120
           KILSSYEKALS  SS GIQISESPSS NGSPRSEDSDREFKD D    +SR RN LPTWT
Sbjct: 61  KILSSYEKALSLLSSNGIQISESPSSLNGSPRSEDSDREFKDHDHTNASSRTRNILPTWT 120

Query: 121 QKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQRSDD 180
           QKFQVSPGMA EGS DDGFCWRKYGQK ILGA+HPRGYYRCTH+NLQGCLATKQVQRSD 
Sbjct: 121 QKFQVSPGMALEGSFDDGFCWRKYGQKGILGARHPRGYYRCTHRNLQGCLATKQVQRSDH 180

Query: 181 DPTVFEITYRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQ---NQQS 240
           DP VFEITYRG H+C+QV     PSA+ EFQQ N   +           +PDQ   NQQ+
Sbjct: 181 DPNVFEITYRGTHSCTQVI----PSASTEFQQQNHPLL-----------LPDQRVQNQQT 240

Query: 241 SPDALLNSWASLRVITENLDT-HEPTLFPAFSYDPTSSYKAADHVESAS-MTVGVNFAEF 300
           SPDALL+SW SLRVITENLDT H+PTLF  F+YDPTS+Y+AAD VES S +   VNF+EF
Sbjct: 241 SPDALLSSWPSLRVITENLDTTHDPTLFHPFNYDPTSNYEAADCVESGSTLRANVNFSEF 300

Query: 301 -PPSFLSPTTSCSGLSYFSASSGGLSEGFVGNQK---LQANKSELS--FPSPTSALNSQT 360
            PP FLSPTTS SGLSYFSASS GLSEGF+G+ +   LQ NKSELS  F SPTSA NS T
Sbjct: 301 SPPPFLSPTTSGSGLSYFSASSSGLSEGFIGHHQKLDLQPNKSELSEIFSSPTSASNSAT 360

Query: 361 LGSEFPFGEVEMEPSFTFDHTNFFS 365
            G +FPFGE++MEP+FTF +TNFFS
Sbjct: 361 PGLDFPFGELQMEPTFTFHNTNFFS 370

BLAST of MC10g0078 vs. ExPASy TrEMBL
Match: A0A6J1CH11 (probable WRKY transcription factor 53 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011439 PE=4 SV=1)

HSP 1 Score: 724 bits (1870), Expect = 9.59e-264
Identity = 365/365 (100.00%), Postives = 365/365 (100.00%), Query Frame = 0

Query: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60
           MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA
Sbjct: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60

Query: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120
           LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM
Sbjct: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120

Query: 121 APEGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITY 180
           APEGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITY
Sbjct: 121 APEGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITY 180

Query: 181 RGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWASL 240
           RGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWASL
Sbjct: 181 RGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWASL 240

Query: 241 RVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSGL 300
           RVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSGL
Sbjct: 241 RVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSGL 300

Query: 301 SYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFDH 360
           SYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFDH
Sbjct: 301 SYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFDH 360

Query: 361 TNFFS 365
           TNFFS
Sbjct: 361 TNFFS 365

BLAST of MC10g0078 vs. ExPASy TrEMBL
Match: A0A6J1CJ16 (probable WRKY transcription factor 53 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011439 PE=4 SV=1)

HSP 1 Score: 720 bits (1858), Expect = 6.72e-262
Identity = 365/366 (99.73%), Postives = 365/366 (99.73%), Query Frame = 0

Query: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60
           MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA
Sbjct: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60

Query: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120
           LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM
Sbjct: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120

Query: 121 APEGSLDDGFCWRKYGQKDILGAKHP-RGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180
           APEGSLDDGFCWRKYGQKDILGAKHP RGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT
Sbjct: 121 APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180

Query: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240
           YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS
Sbjct: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240

Query: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300
           LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG
Sbjct: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300

Query: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360
           LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD
Sbjct: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360

Query: 361 HTNFFS 365
           HTNFFS
Sbjct: 361 HTNFFS 366

BLAST of MC10g0078 vs. ExPASy TrEMBL
Match: A0A6J1CGF2 (probable WRKY transcription factor 53 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111011439 PE=4 SV=1)

HSP 1 Score: 711 bits (1834), Expect = 2.84e-258
Identity = 363/366 (99.18%), Postives = 363/366 (99.18%), Query Frame = 0

Query: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60
           MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA
Sbjct: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKA 60

Query: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGM 120
           LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRK  SLPTWTQKFQVSPGM
Sbjct: 61  LSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRK--SLPTWTQKFQVSPGM 120

Query: 121 APEGSLDDGFCWRKYGQKDILGAKHP-RGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180
           APEGSLDDGFCWRKYGQKDILGAKHP RGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT
Sbjct: 121 APEGSLDDGFCWRKYGQKDILGAKHPSRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEIT 180

Query: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240
           YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS
Sbjct: 181 YRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWAS 240

Query: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300
           LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG
Sbjct: 241 LRVITENLDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSG 300

Query: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360
           LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD
Sbjct: 301 LSYFSASSGGLSEGFVGNQKLQANKSELSFPSPTSALNSQTLGSEFPFGEVEMEPSFTFD 360

Query: 361 HTNFFS 365
           HTNFFS
Sbjct: 361 HTNFFS 364

BLAST of MC10g0078 vs. ExPASy TrEMBL
Match: A0A3T0I8G0 (WRKY transcription factor 13 OS=Siraitia grosvenorii OX=190515 GN=WRKY13 PE=2 SV=1)

HSP 1 Score: 520 bits (1338), Expect = 1.23e-182
Identity = 303/383 (79.11%), Postives = 324/383 (84.60%), Query Frame = 0

Query: 1   MENIGEWEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSED---------GELLVQ 60
           MENIGEWEQKNLKNELLRGKELA+QLQ+YLNV+ SSSM AS S           GELLV+
Sbjct: 1   MENIGEWEQKNLKNELLRGKELARQLQIYLNVRQSSSMAASSSSSSSSSSSNGGGELLVK 60

Query: 61  KILSSYEKALSFFSS-TGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTW 120
           KILSSYEKALS  SS TGIQISESPS+ NGSPRSEDSD      DQR NASRKRN LPTW
Sbjct: 61  KILSSYEKALSLLSSSTGIQISESPSTLNGSPRSEDSDH-----DQR-NASRKRNILPTW 120

Query: 121 TQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQRSD 180
           TQKFQVSPGMA EGSLDDGFCWRKYGQK ILGAKHPRGYYRCTH+N+QGCLATKQVQR D
Sbjct: 121 TQKFQVSPGMALEGSLDDGFCWRKYGQKGILGAKHPRGYYRCTHRNVQGCLATKQVQRCD 180

Query: 181 DDPTVFEITYRGNHTCSQVTNLSPPSAAPE-FQQPNSSNIDPNLVQKQSHSVPDQNQQSS 240
           DDPTVFEITYRGNHTCSQV+NL+PPSA P+ FQQPNSS ID +LVQ  SH  PDQNQQ+S
Sbjct: 181 DDPTVFEITYRGNHTCSQVSNLTPPSATPDQFQQPNSS-ID-SLVQ--SH-PPDQNQQTS 240

Query: 241 P-DALLNSWASLRVITENLDT-HEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFP 300
           P DALLNSWASL+VITENLDT HEPT FP FSYDPTS+Y+AA+HV+SAS TV VNFAEF 
Sbjct: 241 PPDALLNSWASLKVITENLDTTHEPT-FPPFSYDPTSNYEAANHVKSAS-TVDVNFAEFS 300

Query: 301 PSFLSPTT--SCSGLSYFSASSGGLSEGFVGNQKLQANKSELS--FPSPTSALNSQTLGS 360
           PSF+SPTT  S SGL YF ASS  LSEGFVG+Q LQANK ELS  F SPTSA+NSQTLG 
Sbjct: 301 PSFISPTTTTSGSGLRYFPASSSVLSEGFVGHQNLQANKPELSEIFSSPTSAVNSQTLGL 360

Query: 361 EFPFGEVE-MEPSFTFDHTNFFS 365
           EFPFGEVE MEPSFTF +TNFFS
Sbjct: 361 EFPFGEVELMEPSFTFHNTNFFS 370

BLAST of MC10g0078 vs. ExPASy TrEMBL
Match: A0A6J1E4E2 (probable WRKY transcription factor 53 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430671 PE=4 SV=1)

HSP 1 Score: 473 bits (1216), Expect = 5.81e-164
Identity = 266/391 (68.03%), Postives = 300/391 (76.73%), Query Frame = 0

Query: 1   MENIGEWEQKN-LKNELLRGKELAKQLQLYLNVQPSSSMPASPS---------------- 60
           M++ GEW+QK  LKNELL+GKELAKQLQ++LNV+PSSS  A+ +                
Sbjct: 1   MDSFGEWDQKKKLKNELLKGKELAKQLQIHLNVRPSSSSMAAAAAAAAAVSSSSSSSVSN 60

Query: 61  EDGELLVQKILSSYEKALSFFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRK 120
           + GELLVQKILSSYEKALS  SS GIQISESPSS NGSPRSEDSDREFKD D    +SR 
Sbjct: 61  DGGELLVQKILSSYEKALSLLSSNGIQISESPSSLNGSPRSEDSDREFKDHDHTNASSRT 120

Query: 121 RNSLPTWTQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLAT 180
           RN LPTWTQKFQVSPGMA EGSLDDGFCWRKYGQK ILGA+HPRGYYRCTH+NLQGCLAT
Sbjct: 121 RNILPTWTQKFQVSPGMALEGSLDDGFCWRKYGQKGILGARHPRGYYRCTHRNLQGCLAT 180

Query: 181 KQVQRSDDDPTVFEITYRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPD 240
           KQVQRSD DP +FEITYRG H+C+QV     PSA+ EFQQ N   +    V         
Sbjct: 181 KQVQRSDHDPNIFEITYRGTHSCTQVI----PSASTEFQQQNHPLLPDQRV--------- 240

Query: 241 QNQQSSPDALLNSWASLRVITENLDT-HEPTLFPAFSYDPTSSYKAADHVESAS-MTVGV 300
           QNQQ+SPDALL+SW SLRVITENLDT H+PTLF  F+YDPTS+Y+ AD VES S +   V
Sbjct: 241 QNQQTSPDALLSSWPSLRVITENLDTIHDPTLFHPFNYDPTSNYEVADRVESGSTLRANV 300

Query: 301 NFAEF--PPSFLSPTTSCSGLSYFSASSGGLSEGFVGNQK---LQANKSELS--FPSPTS 360
           NF+EF  PP FLSPTTS SGLSYFSASS GLSEGF+G+ +   LQ NKSELS  F SPTS
Sbjct: 301 NFSEFSPPPPFLSPTTSGSGLSYFSASSSGLSEGFIGHHQKLDLQPNKSELSEIFSSPTS 360

Query: 361 ALNSQTLGSEFPFGEVEMEPSFTFDHTNFFS 365
           A NS T G +FPFGE++MEP+FTF +TNFFS
Sbjct: 361 ASNSATPGLDFPFGELQMEPTFTFHNTNFFS 378

BLAST of MC10g0078 vs. TAIR 10
Match: AT4G23810.1 (WRKY family transcription factor )

HSP 1 Score: 191.0 bits (484), Expect = 1.6e-48
Identity = 128/304 (42.11%), Postives = 169/304 (55.59%), Query Frame = 0

Query: 7   WEQKNLKNELLRGKELAKQLQLYLNVQPS-----SSMPASPSEDGELLVQKILSSYEKA- 66
           WEQK L +EL+ G + AK+LQ  L   PS     SS   + +E  E+LV++I+SSYE++ 
Sbjct: 9   WEQKTLLSELINGFDAAKKLQARLREAPSPSSSFSSPATAVAETNEILVKQIVSSYERSL 68

Query: 67  --LSFFSSTGIQ----------------ISESPSSPNGSPRSED------SDREFKDQDQ 126
             L++ SS  +Q                + ESP+S NGSPRSE+      S      QD 
Sbjct: 69  LLLNWSSSPSVQLIPTPVTVVPVANPGSVPESPASINGSPRSEEFADGGGSSESHHRQDY 128

Query: 127 RINASRKRNSLPTWTQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKN 186
             N S+KR  LP W++K ++SP    EG  DD F WRKYGQKDILGAK PR YYRCTH++
Sbjct: 129 IFN-SKKRKMLPKWSEKVRISPERGLEGPQDDVFSWRKYGQKDILGAKFPRSYYRCTHRS 188

Query: 187 LQGCLATKQVQRSDDDPTVFEITYRGNHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQK 246
            Q C ATKQVQRSD D TVFE+TYRG HTCSQ    +PP A+PE +Q   + + P + QK
Sbjct: 189 TQNCWATKQVQRSDGDATVFEVTYRGTHTCSQAITRTPPLASPEKRQ--DTRVKPAITQK 248

Query: 247 QSHSVPDQNQQSSPDALLNSWASLRVITENLDTHE-----PTLFPAFSYDPTSSYKAADH 276
                         D L +  ++L V T+ LD  +     P   P ++Y   +      H
Sbjct: 249 PK------------DILESLKSNLTVRTDGLDDGKDVFSFPDTPPFYNYGTING--EFGH 295

BLAST of MC10g0078 vs. TAIR 10
Match: AT4G11070.1 (WRKY family transcription factor )

HSP 1 Score: 163.7 bits (413), Expect = 2.8e-40
Identity = 93/200 (46.50%), Postives = 123/200 (61.50%), Query Frame = 0

Query: 7   WEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKALSFFSS 66
           WE+++L NEL+ G + AKQLQ   +   S+S     +E  E L+  I+SS++KA+   + 
Sbjct: 6   WERRSLLNELIHGLKAAKQLQGSSSPSLSASSSYLTTEIKENLLHNIVSSFKKAILMLNG 65

Query: 67  TGIQ-----------------ISESPSSPNGSPRSEDSDREFKDQDQRIN-ASRKRNSLP 126
           +  Q                 +  SP+S  G+PRSE+    F  + +  N +S+KR  LP
Sbjct: 66  STTQHNPTIELAPDPLAHPGKVPGSPASITGNPRSEEF---FNVRSKEFNLSSKKRKMLP 125

Query: 127 TWTQKFQVSPGMAPEGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQR 186
            WT++ ++SP    EG  DD F WRKYGQKDILGAK PR YYRCT +N Q C ATKQVQR
Sbjct: 126 KWTEQVRISPERGLEGPHDDIFSWRKYGQKDILGAKFPRSYYRCTFRNTQYCWATKQVQR 185

Query: 187 SDDDPTVFEITYRGNHTCSQ 189
           SD DPT+FE+TYRG HTCSQ
Sbjct: 186 SDGDPTIFEVTYRGTHTCSQ 202

BLAST of MC10g0078 vs. TAIR 10
Match: AT5G24110.1 (WRKY DNA-binding protein 30 )

HSP 1 Score: 153.3 bits (386), Expect = 3.8e-37
Identity = 113/306 (36.93%), Postives = 164/306 (53.59%), Query Frame = 0

Query: 5   GEWEQ-KNLKNEL-LRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKALS 64
           GEWE+ KN  NEL + G++ A Q              AS  E  E L +KIL SY K+L+
Sbjct: 8   GEWEKMKNEINELMIEGRDYAHQFG-----------SASSQETREHLAKKILQSYHKSLT 67

Query: 65  FFSSTGIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGMAP 124
             + +G    +  S   GSP+S+DSD+E       I +S+K  S+P W+ K +++PG   
Sbjct: 68  IMNYSG--ELDQVSQGGGSPKSDDSDQE----PLVIKSSKK--SMPRWSSKVRIAPGAGV 127

Query: 125 EGSLDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITYRG 184
           + +LDDGF WRKYGQKDILGAK PRGYYRCT++  QGC ATKQVQRSD++  + EI+YRG
Sbjct: 128 DRTLDDGFSWRKYGQKDILGAKFPRGYYRCTYRKSQGCEATKQVQRSDENQMLLEISYRG 187

Query: 185 NHTCSQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQ------------SSP 244
            H+CSQ  N+          +PN +    NL      SV + N Q            S+P
Sbjct: 188 IHSCSQAANVGTTMPIQNL-EPNQTQEHGNL-DMVKESVDNYNHQAHLHHNLHYPLSSTP 247

Query: 245 DALLNSWASLRVITENLDTHEPTLF-----PAFSYDPTSSYKAADHVESASMTVGVN--F 290
           +   N+   L++  +N++    T F      + +Y+  +S  A+    ++  TV +   F
Sbjct: 248 NLENNNAYMLQMRDQNIEYFGSTSFSSDLGTSINYNFPASGSASHSASNSPSTVPLESPF 292

BLAST of MC10g0078 vs. TAIR 10
Match: AT4G11070.2 (WRKY family transcription factor )

HSP 1 Score: 151.4 bits (381), Expect = 1.4e-36
Identity = 87/183 (47.54%), Postives = 113/183 (61.75%), Query Frame = 0

Query: 7   WEQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKALSFFSS 66
           WE+++L NEL+ G + AKQLQ  L +  S++                  + E A    + 
Sbjct: 6   WERRSLLNELIHGLKAAKQLQAILMLNGSTTQHN--------------PTIELAPDPLAH 65

Query: 67  TGIQISESPSSPNGSPRSEDSDREFKDQDQRIN-ASRKRNSLPTWTQKFQVSPGMAPEGS 126
            G ++  SP+S  G+PRSE+    F  + +  N +S+KR  LP WT++ ++SP    EG 
Sbjct: 66  PG-KVPGSPASITGNPRSEEF---FNVRSKEFNLSSKKRKMLPKWTEQVRISPERGLEGP 125

Query: 127 LDDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITYRGNHT 186
            DD F WRKYGQKDILGAK PR YYRCT +N Q C ATKQVQRSD DPT+FE+TYRG HT
Sbjct: 126 HDDIFSWRKYGQKDILGAKFPRSYYRCTFRNTQYCWATKQVQRSDGDPTIFEVTYRGTHT 170

Query: 187 CSQ 189
           CSQ
Sbjct: 186 CSQ 170

BLAST of MC10g0078 vs. TAIR 10
Match: AT2G46400.1 (WRKY DNA-binding protein 46 )

HSP 1 Score: 123.2 bits (308), Expect = 4.2e-28
Identity = 103/295 (34.92%), Postives = 156/295 (52.88%), Query Frame = 0

Query: 8   EQKNLKNELLRGKELAKQLQLYLNVQPSSSMPASPSEDGELLVQKILSSYEKALSFFSST 67
           E+K + NEL  GKELA +L    N++ +SS+     +  + L+  IL  Y+ A+   S  
Sbjct: 4   EEKLVINELELGKELANRLM--NNLKHTSSV-----DSNKTLISDILRIYQNAIFMLSFN 63

Query: 68  GIQISESPSSPNGSPRSEDSDREFKDQDQRINASRKRNSLPTWTQKFQVSPGMAPE-GSL 127
                      N   RS + D     +D + N  +KR      T+K +V      E GS+
Sbjct: 64  --------QDKNILKRSLEID----GKDSK-NVFKKRKVSEKNTEKVKVFVATEQENGSI 123

Query: 128 DDGFCWRKYGQKDILGAKHPRGYYRCTHKNLQGCLATKQVQRSDDDPTVFEITYRGNHTC 187
           DDG CWRKYGQK+I G+K+PR YYRCTH+  Q CLA KQVQ+SD DP++FE+ Y GNHTC
Sbjct: 124 DDGHCWRKYGQKEIHGSKNPRAYYRCTHRFTQDCLAVKQVQKSDTDPSLFEVKYLGNHTC 183

Query: 188 SQVTNLSPPSAAPEFQQPNSSNIDPNLVQKQSHSVPDQNQQSSPDALLNSWASLRVITEN 247
           + +T+   P     F   + S  + N+ +     V +Q++   P        SL    E+
Sbjct: 184 NNITS---PKTTTNF---SVSLTNTNIFEGNRVHVTEQSEDMKPTKSEEVMISL----ED 243

Query: 248 LDTHEPTLFPAFSYDPTSSYKAADHVESASMTVGVNFAEFPPSFLSPTTSCSGLS 302
           L+ ++  +F  FS+   S+++  + V  +++ +G NF E     LSP TS S ++
Sbjct: 244 LE-NKKNIFRTFSF---SNHEIENGVWKSNLFLG-NFVE----DLSPATSGSAIT 259

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SUP62.3e-4742.11Probable WRKY transcription factor 53 OS=Arabidopsis thaliana OX=3702 GN=WRKY53 ... [more]
Q8H0Y83.9e-3946.50Probable WRKY transcription factor 41 OS=Arabidopsis thaliana OX=3702 GN=WRKY41 ... [more]
Q9FL625.3e-3636.93Probable WRKY transcription factor 30 OS=Arabidopsis thaliana OX=3702 GN=WRKY30 ... [more]
Q9SKD95.9e-2734.92Probable WRKY transcription factor 46 OS=Arabidopsis thaliana OX=3702 GN=WRKY46 ... [more]
Q0DFT72.1e-2449.56Transcription factor WRKY19 OS=Oryza sativa subsp. japonica OX=39947 GN=WRKY19 P... [more]
Match NameE-valueIdentityDescription
XP_022140884.11.98e-263100.00probable WRKY transcription factor 53 isoform X2 [Momordica charantia][more]
XP_022140883.11.39e-26199.73probable WRKY transcription factor 53 isoform X1 [Momordica charantia][more]
XP_022140885.15.86e-25899.18probable WRKY transcription factor 53 isoform X3 [Momordica charantia][more]
AZU90763.12.54e-18279.11WRKY transcription factor 13 [Siraitia grosvenorii] >QEE82235.1 WRKY transcripti... [more]
KAG6576671.12.23e-16470.13putative WRKY transcription factor 53, partial [Cucurbita argyrosperma subsp. so... [more]
Match NameE-valueIdentityDescription
A0A6J1CH119.59e-264100.00probable WRKY transcription factor 53 isoform X2 OS=Momordica charantia OX=3673 ... [more]
A0A6J1CJ166.72e-26299.73probable WRKY transcription factor 53 isoform X1 OS=Momordica charantia OX=3673 ... [more]
A0A6J1CGF22.84e-25899.18probable WRKY transcription factor 53 isoform X3 OS=Momordica charantia OX=3673 ... [more]
A0A3T0I8G01.23e-18279.11WRKY transcription factor 13 OS=Siraitia grosvenorii OX=190515 GN=WRKY13 PE=2 SV... [more]
A0A6J1E4E25.81e-16468.03probable WRKY transcription factor 53 isoform X1 OS=Cucurbita moschata OX=3662 G... [more]
Match NameE-valueIdentityDescription
AT4G23810.11.6e-4842.11WRKY family transcription factor [more]
AT4G11070.12.8e-4046.50WRKY family transcription factor [more]
AT5G24110.13.8e-3736.93WRKY DNA-binding protein 30 [more]
AT4G11070.21.4e-3647.54WRKY family transcription factor [more]
AT2G46400.14.2e-2834.92WRKY DNA-binding protein 46 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 126..188
e-value: 3.2E-42
score: 156.2
IPR003657WRKY domainPFAMPF03106WRKYcoord: 127..186
e-value: 1.1E-26
score: 92.9
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 121..184
score: 22.802212
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 112..181
e-value: 1.5E-27
score: 97.0
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 124..188
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 70..109
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 83..100
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 189..230
NoneNo IPR availablePANTHERPTHR32096:SF36WRKY TRANSCRIPTION FACTOR 30-RELATEDcoord: 1..362
IPR044810WRKY transcription factor, plantPANTHERPTHR32096WRKY TRANSCRIPTION FACTOR 30-RELATED-RELATEDcoord: 1..362

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC10g0078.1MC10g0078.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0000976 transcription cis-regulatory region binding
molecular_function GO:0043565 sequence-specific DNA binding