MC10g0030 (gene) Bitter gourd (Dali-11) v1

BLAST of MC10g0030 vs. ExPASy Swiss-Prot
Match: Q94BY9 (Rab GTPase-activating protein 22 OS=Arabidopsis thaliana OX=3702 GN=RABGAP22 PE=1 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 2.9e-175
Identity = 365/593 (61.55%), Postives = 437/593 (73.69%), Query Frame = 0

Query: 49  YSASRALKRTQTPSSPDPNSNSPPSSSSSSSASSSAPS---SSSSSSSSSWVHLRSVLFV 108
           Y+++ +   + + S P  +S+S PSSSSSS  SS++ S   S+SSSSSSSW+HLRSVLFV
Sbjct: 8   YTSTSSGNSSSSSSLPSSSSSSLPSSSSSSPPSSNSNSYSNSNSSSSSSSWIHLRSVLFV 67

Query: 109 VTSSSPASSSSSDRGRLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGG 168
              SSP+S +SSDR R KSPWSR+KRK ALTP QWRSLFTP+GKLRDGG+ FLKKVRS G
Sbjct: 68  ANLSSPSSVTSSDR-RRKSPWSRRKRKWALTPHQWRSLFTPEGKLRDGGVGFLKKVRSRG 127

Query: 169 VDPSIRAEVWPFLLGVYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDD 228
           VDPSIRAEVW FLLGVYDL+S+ EER+AV+ QKRKEYE+L+++C+ LLK G+ ST     
Sbjct: 128 VDPSIRAEVWLFLLGVYDLNSTSEEREAVKTQKRKEYEKLQRRCQMLLKCGNGST----- 187

Query: 229 DMNSNKERDENIKLNDGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRN 288
                                    DN                   E + SDE  S    
Sbjct: 188 -------------------------DN------------------LEELPSDEANSQCVR 247

Query: 289 LDGTSGVFLEEDDSSRRMTSAD-ASAPNTESSDSDSSEDPE---VSQTFPSSDGR---ED 348
                  F+++   +  MTS D  SA NT+SSD+DS ED E   +  +F  SD +   ED
Sbjct: 248 -------FVDDYKITGPMTSQDVVSALNTDSSDTDSCEDNEDVLLLSSFAHSDEKKPEED 307

Query: 349 NDPDFTSKNSSPSVA---EVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSD 408
           N  + + +NSS  VA   EV  +   +EDF+TWQRIIRLDA+RA+SEW  Y+P   A+++
Sbjct: 308 NSNNNSEENSSLLVAAASEVQVEVAVHEDFSTWQRIIRLDALRADSEWANYSPYSTAITE 367

Query: 409 DKARHSAEVVGLKDYDHLEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVI 468
            KAR  AE VGLKDYDHLE  R++HAARLV ILEAYA+YDPEIGYCQGMSDLLSPI+ VI
Sbjct: 368 SKARRLAESVGLKDYDHLESCRLYHAARLVAILEAYAMYDPEIGYCQGMSDLLSPILAVI 427

Query: 469 TEDQEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFF 528
           +ED EAFWCFVGFM+KARHNFRLDE GI++QL+IVS+IIK KDS LY+HLE LQAEDC F
Sbjct: 428 SEDHEAFWCFVGFMKKARHNFRLDEAGIQRQLSIVSKIIKNKDSQLYKHLENLQAEDCSF 487

Query: 529 VYRMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIA 588
           VYRMV+V+FRREL+FEQTLCLWEVMWADQAAIRAGVGKS WSRIRQ+APPT+DLLLYAIA
Sbjct: 488 VYRMVLVMFRRELSFEQTLCLWEVMWADQAAIRAGVGKSPWSRIRQQAPPTDDLLLYAIA 543

Query: 589 ASVLQKRKLIIEKYNSMDEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKIET 629
           A VL +RKLII+KY+SMDEI+ ECNSMAGQL++WKLLDDAH LVVTLH+KIET
Sbjct: 548 ALVL-RRKLIIQKYSSMDEIVEECNSMAGQLNVWKLLDDAHHLVVTLHDKIET 543

BLAST of MC10g0030 vs. ExPASy Swiss-Prot
Match: P09379 (GTPase-activating protein GYP7 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=GYP7 PE=3 SV=2)

HSP 1 Score: 122.9 bits (307), Expect = 1.3e-26
Identity = 65/208 (31.25%), Postives = 109/208 (52.40%), Query Frame = 0

Query: 420 HAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKARHNFRLD 479
           H  +L  +L  Y  ++  +GY QGMSDLLSP+  V+ +D  AFW F  FM +   N+  D
Sbjct: 495 HLIQLRDMLITYNQHNKNLGYVQGMSDLLSPLYVVLQDDTLAFWAFSAFMERMERNYLRD 554

Query: 480 EVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEV 539
           + G+R QL  +  +++F    LY+HLEK ++ + FF +RM++V F+REL ++  L LWEV
Sbjct: 555 QSGMRNQLLCLDHLVQFMLPSLYKHLEKTESTNLFFFFRMLLVWFKRELLWDDVLRLWEV 614

Query: 540 MWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIREC 599
           +W D                      +   +L+   A + + + ++I+     DEI++  
Sbjct: 615 LWTDYL--------------------SSQFVLFVCLAILDKHKDVMIDHLAGFDEILKYM 674

Query: 600 NSMAGQLDIWKLLDDAHDLVVTLHEKIE 628
           N ++  +D+ +LL  A  L       +E
Sbjct: 675 NELSMTIDLDELLVRAELLFYRFRRTVE 682

HSP 2 Score: 21.2 bits (43), Expect = 5.5e+04
Identity = 53/204 (25.98%), Postives = 89/204 (43.63%), Query Frame = 0

Query: 10  PSFGVGGGLVSRMMAGARPNAAV--VAFTALAGLAVVLVLFYSASRALKRTQTPSSPDPN 69
           P  G GG  V R   GA+   A   + +  L+ LA V  L    +R + +    SSP P 
Sbjct: 235 PGRGAGGDPVDR---GAQVQKAFSDIRWGLLSNLAKVTQL----TRKVSQGVWDSSPQPV 294

Query: 70  SN---SPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSPASSSSSDRGRLKS 129
                 P          S+    +  + S +    R+ L V+         S +   L  
Sbjct: 295 KQLLMKPEVKKIGDDFDSARIYLAKWALSVAEESQRAKLKVLFDDELRELVSDEGFELID 354

Query: 130 PWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYDL 189
             +  +R+N ++  +W + F  +G+L     +  +++  GG+ P++R E W FLLGVY  
Sbjct: 355 AENNPQRRNEVSLAEWNAFFDYNGRLIVTVNEVKERIFHGGLAPAVRPEGWLFLLGVYPW 414

Query: 190 SSSEEERDAVRLQKRKEYERLRKQ 209
            S+  ER  +  + R +Y RL+K+
Sbjct: 415 DSTAAERKELVSKLRVDYNRLKKE 431

BLAST of MC10g0030 vs. ExPASy Swiss-Prot
Match: Q9UUH7 (GTPase-activating protein gyp7 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gyp7 PE=3 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 8.1e-24
Identity = 64/232 (27.59%), Postives = 115/232 (49.57%), Query Frame = 0

Query: 380 NSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLEPSRIF-----HAARLVTILEAYALY 439
           N  W     ++      +     E   ++D  H +P   F     +   +  IL  Y  Y
Sbjct: 464 NDRWFIEQRNRIEKDVHRTDRQHEYFQIEDLPHPDPQSTFTGTNMNMEMMKDILLTYNEY 523

Query: 440 DPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRII 499
           D E+GY QGMSDLL+PI     ++   FW  VG M++   NF  D+ G+ +QL+ +  +I
Sbjct: 524 DTELGYVQGMSDLLAPIYVTFNDNALTFWGMVGLMKRLHFNFLRDQSGMHRQLDTLRLLI 583

Query: 500 KFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKS 559
           +F D  L+ HLEK  + + F  +RM+++ F+RE  +E  L LW+V++ +  +    +   
Sbjct: 584 EFMDPELFAHLEKTDSSNLFCFFRMLLIYFKREFDWEVLLKLWDVLFTNYLSYDYHI--- 643

Query: 560 AWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRECNSMAGQL 607
                          + YAIA    + R++++ + ++ DE+++  N ++G+L
Sbjct: 644 --------------FVAYAIAE---RHREVLLNQTSAFDEVLKYFNELSGKL 675

HSP 2 Score: 67.8 bits (164), Expect = 5.1e-10
Identity = 34/81 (41.98%), Postives = 50/81 (61.73%), Query Frame = 0

Query: 128 RKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYDLSSS 187
           R KR + L+ +QW S+F   GKL+    + L  +  GG+ PS+R EVWPFLL VY   S+
Sbjct: 374 RVKRDDPLSVEQWNSMFNAHGKLQVDVHRVLGIIFHGGIQPSLRKEVWPFLLSVYPWDST 433

Query: 188 EEERDAVRLQKRKEYERLRKQ 209
            EER  + L  ++EY  L+++
Sbjct: 434 SEERRVIYLSLQEEYCTLKRK 454

BLAST of MC10g0030 vs. ExPASy Swiss-Prot
Match: Q6BU76 (GTPase-activating protein GYP7 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=GYP7 PE=3 SV=2)

HSP 1 Score: 111.3 bits (277), Expect = 4.0e-23
Identity = 69/237 (29.11%), Postives = 121/237 (51.05%), Query Frame = 0

Query: 401 SAEVVGLKDYDHLEPSRIF--HAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED 460
           S E     D D  + S I   H  ++  IL  Y  ++  +GY QGM+DLLSP+     ++
Sbjct: 495 SPETPDEADDDEFDVSNITNPHLFKMREILLTYNEHNVNLGYVQGMTDLLSPLYVTFQDE 554

Query: 461 QEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYR 520
              FW FV FM +   NF  D+ G++ Q+  ++ +++F    L++HLEK ++ D +F +R
Sbjct: 555 SLTFWAFVNFMDRMERNFLRDQSGMKNQMLTLNELVQFMLPDLFKHLEKCESTDLYFFFR 614

Query: 521 MVVVLFRRELTFEQTLCLWEVMWADQAA------IRAGVGKSAWSRIRQRAPPTEDLLLY 580
           M++V F+RE  +   L LWE++W D  +          V       IRQ     +++L Y
Sbjct: 615 MLLVWFKREFEWSSVLSLWEILWTDYYSGQFHLFFALAVLSDNERIIRQNLSRFDEVLKY 674

Query: 581 AIAAS-------VLQKRKLIIEKYNSMDEIIRECNSMAGQLDIWKLLDDAHDLVVTL 623
               S       +L + +L+  ++  M +II   NS+  +L+   + DDA++ ++ +
Sbjct: 675 MNDLSMNMNLNHLLIRAELLFLRFRRMIDIIDRENSLK-KLNNPGVYDDANNSLIKI 730

HSP 2 Score: 45.4 bits (106), Expect = 2.7e-03
Identity = 33/109 (30.28%), Postives = 51/109 (46.79%), Query Frame = 0

Query: 127 SRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYDLSS 186
           S+  R+  ++  +W  LF   G+L     +   ++  GG++  IR E W FLL VY   S
Sbjct: 357 SKTSRRKPISKVEWEGLFDFSGRLIISIDEIKDRIFHGGLEDCIRGEAWLFLLNVYPWDS 416

Query: 187 SEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKERDE 236
           S EER  +R   +  YE ++      LK  ++  K + D     K R E
Sbjct: 417 SAEERKTLRNSFQTAYEEIK------LKWVNDDDKRSVDFWKDQKHRIE 459

BLAST of MC10g0030 vs. ExPASy Swiss-Prot
Match: Q8TC07 (TBC1 domain family member 15 OS=Homo sapiens OX=9606 GN=TBC1D15 PE=1 SV=2)

HSP 1 Score: 110.5 bits (275), Expect = 6.8e-23
Identity = 57/186 (30.65%), Postives = 99/186 (53.23%), Query Frame = 0

Query: 427 ILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKARHNFRLDEVGIRKQ 486
           IL  Y +YD ++GY QGMSDLLSP++ V+  + +AFWCF  +M +   NF     G++ Q
Sbjct: 439 ILMTYCMYDFDLGYVQGMSDLLSPLLYVMENEVDAFWCFASYMDQMHQNFEEQMQGMKTQ 498

Query: 487 LNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWADQAA 546
           L  +S +++  DS    +LE   +   +F +R +++ F+RE +F   L LWEVMW +   
Sbjct: 499 LIQLSTLLRLLDSGFCSYLESQDSGYLYFCFRWLLIRFKREFSFLDILRLWEVMWTEL-- 558

Query: 547 IRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRECNSMAGQL 606
                             P  +  L    A +  +++ I+EK+   +EI++  N ++ ++
Sbjct: 559 ------------------PCTNFHLLLCCAILESEKQQIMEKHYGFNEILKHINELSMKI 604

Query: 607 DIWKLL 613
           D+  +L
Sbjct: 619 DVEDIL 604

BLAST of MC10g0030 vs. NCBI nr
Match: XP_022141059.1 (GTPase-activating protein GYP7-like isoform X1 [Momordica charantia])

HSP 1 Score: 1099 bits (2842), Expect = 0.0
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0

Query: 53  RALKRTQTPSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSPA 112
           RALKRTQTPSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSPA
Sbjct: 2   RALKRTQTPSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSPA 61

Query: 113 SSSSSDRGRLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRA 172
           SSSSSDRGRLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRA
Sbjct: 62  SSSSSDRGRLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRA 121

Query: 173 EVWPFLLGVYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKE 232
           EVWPFLLGVYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKE
Sbjct: 122 EVWPFLLGVYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKE 181

Query: 233 RDENIKLNDGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGV 292
           RDENIKLNDGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGV
Sbjct: 182 RDENIKLNDGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGV 241

Query: 293 FLEEDDSSRRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSV 352
           FLEEDDSSRRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSV
Sbjct: 242 FLEEDDSSRRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSV 301

Query: 353 AEVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDH 412
           AEVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDH
Sbjct: 302 AEVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDH 361

Query: 413 LEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKA 472
           LEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKA
Sbjct: 362 LEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKA 421

Query: 473 RHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQ 532
           RHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQ
Sbjct: 422 RHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQ 481

Query: 533 TLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSM 592
           TLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSM
Sbjct: 482 TLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSM 541

Query: 593 DEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE 632
           DEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE
Sbjct: 542 DEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE 581

BLAST of MC10g0030 vs. NCBI nr
Match: XP_022141060.1 (GTPase-activating protein GYP7-like isoform X2 [Momordica charantia])

HSP 1 Score: 1063 bits (2749), Expect = 0.0
Identity = 566/632 (89.56%), Postives = 566/632 (89.56%), Query Frame = 0

Query: 1   MIASGTDIAPSFGVGGGLVSRMMAGARPNAAVVAFTALAGLAVVLVLFYSASRALKRTQT 60
           MIASGTDIAPSFGVGGGLVSRMMAGARPNAAVVAFTALAGLAVVLVLFYSASR       
Sbjct: 1   MIASGTDIAPSFGVGGGLVSRMMAGARPNAAVVAFTALAGLAVVLVLFYSASR------- 60

Query: 61  PSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSPASSSSSDRG 120
                                                                      G
Sbjct: 61  -----------------------------------------------------------G 120

Query: 121 RLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG 180
           RLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG
Sbjct: 121 RLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG 180

Query: 181 VYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKERDENIKLN 240
           VYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKERDENIKLN
Sbjct: 181 VYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKERDENIKLN 240

Query: 241 DGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGVFLEEDDSS 300
           DGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGVFLEEDDSS
Sbjct: 241 DGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGVFLEEDDSS 300

Query: 301 RRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSVAEVTSKFR 360
           RRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSVAEVTSKFR
Sbjct: 301 RRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSVAEVTSKFR 360

Query: 361 SNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLEPSRIFH 420
           SNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLEPSRIFH
Sbjct: 361 SNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLEPSRIFH 420

Query: 421 AARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKARHNFRLDE 480
           AARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKARHNFRLDE
Sbjct: 421 AARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKARHNFRLDE 480

Query: 481 VGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVM 540
           VGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVM
Sbjct: 481 VGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVM 540

Query: 541 WADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRECN 600
           WADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRECN
Sbjct: 541 WADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRECN 566

Query: 601 SMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE 632
           SMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE
Sbjct: 601 SMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE 566

BLAST of MC10g0030 vs. NCBI nr
Match: XP_038896369.1 (small G protein signaling modulator 1-like isoform X3 [Benincasa hispida])

HSP 1 Score: 1012 bits (2617), Expect = 0.0
Identity = 553/636 (86.95%), Postives = 572/636 (89.94%), Query Frame = 0

Query: 1   MIASGTDIAPSFGVGGGLVSRMMAGARPNAAVVAFTALAGLAVVLVLFYSASRALKRTQT 60
           MIASGTDIAPSFGVGGGL S+MMA ARPNAAV A TALAG AVV+VLFYSASRALKRT T
Sbjct: 1   MIASGTDIAPSFGVGGGLFSQMMAVARPNAAV-AVTALAGFAVVVVLFYSASRALKRTPT 60

Query: 61  PSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSPASSSSS--D 120
            SSPDPNSNSPPSS           SSSSSS+SSSWVHLRSVLFVVTSSSPASSSSS  D
Sbjct: 61  SSSPDPNSNSPPSS-----------SSSSSSASSSWVHLRSVLFVVTSSSPASSSSSSSD 120

Query: 121 RGRLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFL 180
           RGRLKSPWSRKKRK+AL+PQQWR LFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFL
Sbjct: 121 RGRLKSPWSRKKRKHALSPQQWRGLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFL 180

Query: 181 LGVYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKERDENIK 240
           LG YDLSSSEEERDAVR+QKRKEYERLRKQCR LLK G ES KL+DD+MN N        
Sbjct: 181 LGFYDLSSSEEERDAVRVQKRKEYERLRKQCRSLLKFGAESIKLDDDEMNCN-------- 240

Query: 241 LNDGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGVFLEEDD 300
                    KEG NQ+VAHGDD P+LE+VVSARESI SDE+ SNFR LDGTSGV LEEDD
Sbjct: 241 ---------KEGGNQHVAHGDDSPTLEDVVSARESIYSDEKASNFRYLDGTSGVLLEEDD 300

Query: 301 SSRRMTS--ADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSVAEVT 360
           SSRRMTS  ADAS  NTESSDSDSSEDPEVSQTFPSSDGREDNDP+FTS NSSP V EVT
Sbjct: 301 SSRRMTSVDADASVLNTESSDSDSSEDPEVSQTFPSSDGREDNDPEFTSNNSSPLVTEVT 360

Query: 361 SKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLEPS 420
           SKFR NEDFTTWQRIIRLDAVRAN+EWIAYAPS AAVSDD+ARH AEVVGLKDYDHLE  
Sbjct: 361 SKFRCNEDFTTWQRIIRLDAVRANAEWIAYAPSLAAVSDDEARHCAEVVGLKDYDHLESC 420

Query: 421 RIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKARHNF 480
           RIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED EAFWCFVGFMRKARHNF
Sbjct: 421 RIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNF 480

Query: 481 RLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCL 540
           RLDEVGIRKQLNIVSRIIKFKDSHLYRHL+ L+AEDCFFVYRMVVVLFRRELTFEQTLCL
Sbjct: 481 RLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLEAEDCFFVYRMVVVLFRRELTFEQTLCL 540

Query: 541 WEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEII 600
           WEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEII
Sbjct: 541 WEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEII 600

Query: 601 RECNSMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE 632
           RECNSMAGQLD+WKLLDDAHDLVVTLHEKIETSF+E
Sbjct: 601 RECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFDE 607

BLAST of MC10g0030 vs. NCBI nr
Match: XP_022984925.1 (GTPase-activating protein GYP7 isoform X5 [Cucurbita maxima])

HSP 1 Score: 1012 bits (2617), Expect = 0.0
Identity = 547/632 (86.55%), Postives = 572/632 (90.51%), Query Frame = 0

Query: 1   MIASGTDIAPSFGVGGGLVSRMMAGARPNAAVVAFTALAGLAVVLVLFYSASRALKRTQT 60
           MIASGTDIAPSFGV GG  SRMMAGA+PNAAV A TALAGLAV+LVLFYSA RALKRT T
Sbjct: 1   MIASGTDIAPSFGVAGGFFSRMMAGAQPNAAV-AVTALAGLAVLLVLFYSARRALKRTPT 60

Query: 61  PSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSPASSSSSDRG 120
            SSPDPNSNSPPSSSS        PSS+ SS+SSSWVHLRSVLFVVTSSSPASSSSSDRG
Sbjct: 61  SSSPDPNSNSPPSSSS--------PSSALSSASSSWVHLRSVLFVVTSSSPASSSSSDRG 120

Query: 121 RLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG 180
           RLKSPWSRKKRK+AL+PQQWRSLFTPDG+LRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG
Sbjct: 121 RLKSPWSRKKRKHALSPQQWRSLFTPDGRLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG 180

Query: 181 VYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKERDENIKLN 240
            YDLSSSEEERDAVR+QKRKEYERLRKQCR LLK GD   KLNDD+MN            
Sbjct: 181 FYDLSSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDGCIKLNDDEMN------------ 240

Query: 241 DGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGVFLEEDDSS 300
                +NKEGD Q V+HGDD PSLE+V SARESISSDERG+NFR LDG+S V LEED+SS
Sbjct: 241 -----FNKEGDAQLVSHGDDSPSLEDVGSARESISSDERGTNFRYLDGSSEVLLEEDNSS 300

Query: 301 RRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSVAEVTSKFR 360
           R+MT+AD S  NTESSDSDSSEDPEVSQTFPSSDGREDNDP FTSKNSSP V EVTSKFR
Sbjct: 301 RQMTNADVSVLNTESSDSDSSEDPEVSQTFPSSDGREDNDPVFTSKNSSPLVTEVTSKFR 360

Query: 361 SNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLEPSRIFH 420
            NEDFTTWQRIIRLDAVRAN+EWIAYAPS AAVSDDKAR+SAEVVGLKDYDHLE  RIFH
Sbjct: 361 GNEDFTTWQRIIRLDAVRANAEWIAYAPSLAAVSDDKARYSAEVVGLKDYDHLESCRIFH 420

Query: 421 AARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKARHNFRLDE 480
           AARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED EAFWCFVGFMRKARHNFRLDE
Sbjct: 421 AARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLDE 480

Query: 481 VGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVM 540
           VGIRKQLN+VS+II+FKDSHLYRHL+ L+AEDCFFVYRMVVVLFRRELTFEQTLCLWEVM
Sbjct: 481 VGIRKQLNVVSKIIRFKDSHLYRHLQDLEAEDCFFVYRMVVVLFRRELTFEQTLCLWEVM 540

Query: 541 WADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRECN 600
           WADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKY SMDEIIRECN
Sbjct: 541 WADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYYSMDEIIRECN 600

Query: 601 SMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE 632
           SMAGQLD+WKLLDDAHDLVVTLHEKIETSFNE
Sbjct: 601 SMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE 606

BLAST of MC10g0030 vs. NCBI nr
Match: XP_023553419.1 (small G protein signaling modulator 1 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1007 bits (2604), Expect = 0.0
Identity = 544/632 (86.08%), Postives = 565/632 (89.40%), Query Frame = 0

Query: 1   MIASGTDIAPSFGVGGGLVSRMMAGARPNAAVVAFTALAGLAVVLVLFYSASRALKRTQT 60
           MIASGTDIAPSFGV GG  SRMMAGA+PNAAV A TALAGLAV+LVLFYSA RALKRT T
Sbjct: 1   MIASGTDIAPSFGVAGGFFSRMMAGAQPNAAV-AVTALAGLAVLLVLFYSARRALKRTPT 60

Query: 61  PSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSPASSSSSDRG 120
            SSPDPNSNSPPSSSS SSASSS            WVHLRSVLFVVTSSSPASSSSSDRG
Sbjct: 61  SSSPDPNSNSPPSSSSPSSASSS------------WVHLRSVLFVVTSSSPASSSSSDRG 120

Query: 121 RLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG 180
           RLKSPWSRKKRK+AL+PQQWRSLFTPDG+LRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG
Sbjct: 121 RLKSPWSRKKRKHALSPQQWRSLFTPDGRLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG 180

Query: 181 VYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKERDENIKLN 240
            YDLSSSEEERDAVR QKRKEYERLRKQCR  LK GD   KLNDD+MN            
Sbjct: 181 FYDLSSSEEERDAVRGQKRKEYERLRKQCRSFLKFGDGCIKLNDDEMN------------ 240

Query: 241 DGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGVFLEEDDSS 300
                +NKEGD Q V+HGDD PSLE+V SARESISSDERG+NFR LDGTS V L+EDDSS
Sbjct: 241 -----FNKEGDAQLVSHGDDSPSLEDVGSARESISSDERGTNFRYLDGTSEVLLQEDDSS 300

Query: 301 RRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSVAEVTSKFR 360
           R+MT+AD S  NTESSDSDSSEDPEVSQTFPS+DGREDNDP FTSKNS P V EVTSKFR
Sbjct: 301 RQMTNADVSVLNTESSDSDSSEDPEVSQTFPSTDGREDNDPVFTSKNSPPLVTEVTSKFR 360

Query: 361 SNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLEPSRIFH 420
            NEDFTTWQRIIRLDAVRAN+EWIAYAPS AAVSDDKARHSAEVVGLKDYDHLE  RIFH
Sbjct: 361 GNEDFTTWQRIIRLDAVRANAEWIAYAPSLAAVSDDKARHSAEVVGLKDYDHLESCRIFH 420

Query: 421 AARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKARHNFRLDE 480
           AARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED EAFWCFVGFMRKARHNFRLDE
Sbjct: 421 AARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLDE 480

Query: 481 VGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVM 540
           VGIRKQLN+VS+II+FKDSHLYRHL+ L+AEDCFFVYRMVVVLFRRELTFEQTLCLWEVM
Sbjct: 481 VGIRKQLNVVSKIIRFKDSHLYRHLQDLEAEDCFFVYRMVVVLFRRELTFEQTLCLWEVM 540

Query: 541 WADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRECN 600
           WADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKY SMDEIIRECN
Sbjct: 541 WADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYYSMDEIIRECN 600

Query: 601 SMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE 632
           SMAGQLD+WKLLDDAHDLVVTLHEKIETSFNE
Sbjct: 601 SMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE 602

BLAST of MC10g0030 vs. ExPASy TrEMBL
Match: A0A6J1CHH5 (GTPase-activating protein GYP7-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011552 PE=4 SV=1)

HSP 1 Score: 1099 bits (2842), Expect = 0.0
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0

Query: 53  RALKRTQTPSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSPA 112
           RALKRTQTPSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSPA
Sbjct: 2   RALKRTQTPSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSPA 61

Query: 113 SSSSSDRGRLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRA 172
           SSSSSDRGRLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRA
Sbjct: 62  SSSSSDRGRLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRA 121

Query: 173 EVWPFLLGVYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKE 232
           EVWPFLLGVYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKE
Sbjct: 122 EVWPFLLGVYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKE 181

Query: 233 RDENIKLNDGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGV 292
           RDENIKLNDGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGV
Sbjct: 182 RDENIKLNDGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGV 241

Query: 293 FLEEDDSSRRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSV 352
           FLEEDDSSRRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSV
Sbjct: 242 FLEEDDSSRRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSV 301

Query: 353 AEVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDH 412
           AEVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDH
Sbjct: 302 AEVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDH 361

Query: 413 LEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKA 472
           LEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKA
Sbjct: 362 LEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKA 421

Query: 473 RHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQ 532
           RHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQ
Sbjct: 422 RHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQ 481

Query: 533 TLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSM 592
           TLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSM
Sbjct: 482 TLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSM 541

Query: 593 DEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE 632
           DEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE
Sbjct: 542 DEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE 581

BLAST of MC10g0030 vs. ExPASy TrEMBL
Match: A0A6J1CGW8 (GTPase-activating protein GYP7-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011552 PE=4 SV=1)

HSP 1 Score: 1063 bits (2749), Expect = 0.0
Identity = 566/632 (89.56%), Postives = 566/632 (89.56%), Query Frame = 0

Query: 1   MIASGTDIAPSFGVGGGLVSRMMAGARPNAAVVAFTALAGLAVVLVLFYSASRALKRTQT 60
           MIASGTDIAPSFGVGGGLVSRMMAGARPNAAVVAFTALAGLAVVLVLFYSASR       
Sbjct: 1   MIASGTDIAPSFGVGGGLVSRMMAGARPNAAVVAFTALAGLAVVLVLFYSASR------- 60

Query: 61  PSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSPASSSSSDRG 120
                                                                      G
Sbjct: 61  -----------------------------------------------------------G 120

Query: 121 RLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG 180
           RLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG
Sbjct: 121 RLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG 180

Query: 181 VYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKERDENIKLN 240
           VYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKERDENIKLN
Sbjct: 181 VYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKERDENIKLN 240

Query: 241 DGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGVFLEEDDSS 300
           DGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGVFLEEDDSS
Sbjct: 241 DGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGVFLEEDDSS 300

Query: 301 RRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSVAEVTSKFR 360
           RRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSVAEVTSKFR
Sbjct: 301 RRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSVAEVTSKFR 360

Query: 361 SNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLEPSRIFH 420
           SNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLEPSRIFH
Sbjct: 361 SNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLEPSRIFH 420

Query: 421 AARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKARHNFRLDE 480
           AARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKARHNFRLDE
Sbjct: 421 AARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKARHNFRLDE 480

Query: 481 VGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVM 540
           VGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVM
Sbjct: 481 VGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVM 540

Query: 541 WADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRECN 600
           WADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRECN
Sbjct: 541 WADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRECN 566

Query: 601 SMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE 632
           SMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE
Sbjct: 601 SMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE 566

BLAST of MC10g0030 vs. ExPASy TrEMBL
Match: A0A6J1J9X5 (GTPase-activating protein GYP7 isoform X5 OS=Cucurbita maxima OX=3661 GN=LOC111483051 PE=4 SV=1)

HSP 1 Score: 1012 bits (2617), Expect = 0.0
Identity = 547/632 (86.55%), Postives = 572/632 (90.51%), Query Frame = 0

Query: 1   MIASGTDIAPSFGVGGGLVSRMMAGARPNAAVVAFTALAGLAVVLVLFYSASRALKRTQT 60
           MIASGTDIAPSFGV GG  SRMMAGA+PNAAV A TALAGLAV+LVLFYSA RALKRT T
Sbjct: 1   MIASGTDIAPSFGVAGGFFSRMMAGAQPNAAV-AVTALAGLAVLLVLFYSARRALKRTPT 60

Query: 61  PSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSPASSSSSDRG 120
            SSPDPNSNSPPSSSS        PSS+ SS+SSSWVHLRSVLFVVTSSSPASSSSSDRG
Sbjct: 61  SSSPDPNSNSPPSSSS--------PSSALSSASSSWVHLRSVLFVVTSSSPASSSSSDRG 120

Query: 121 RLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG 180
           RLKSPWSRKKRK+AL+PQQWRSLFTPDG+LRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG
Sbjct: 121 RLKSPWSRKKRKHALSPQQWRSLFTPDGRLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG 180

Query: 181 VYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKERDENIKLN 240
            YDLSSSEEERDAVR+QKRKEYERLRKQCR LLK GD   KLNDD+MN            
Sbjct: 181 FYDLSSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDGCIKLNDDEMN------------ 240

Query: 241 DGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGVFLEEDDSS 300
                +NKEGD Q V+HGDD PSLE+V SARESISSDERG+NFR LDG+S V LEED+SS
Sbjct: 241 -----FNKEGDAQLVSHGDDSPSLEDVGSARESISSDERGTNFRYLDGSSEVLLEEDNSS 300

Query: 301 RRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSVAEVTSKFR 360
           R+MT+AD S  NTESSDSDSSEDPEVSQTFPSSDGREDNDP FTSKNSSP V EVTSKFR
Sbjct: 301 RQMTNADVSVLNTESSDSDSSEDPEVSQTFPSSDGREDNDPVFTSKNSSPLVTEVTSKFR 360

Query: 361 SNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDHLEPSRIFH 420
            NEDFTTWQRIIRLDAVRAN+EWIAYAPS AAVSDDKAR+SAEVVGLKDYDHLE  RIFH
Sbjct: 361 GNEDFTTWQRIIRLDAVRANAEWIAYAPSLAAVSDDKARYSAEVVGLKDYDHLESCRIFH 420

Query: 421 AARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKARHNFRLDE 480
           AARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED EAFWCFVGFMRKARHNFRLDE
Sbjct: 421 AARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLDE 480

Query: 481 VGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVM 540
           VGIRKQLN+VS+II+FKDSHLYRHL+ L+AEDCFFVYRMVVVLFRRELTFEQTLCLWEVM
Sbjct: 481 VGIRKQLNVVSKIIRFKDSHLYRHLQDLEAEDCFFVYRMVVVLFRRELTFEQTLCLWEVM 540

Query: 541 WADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRECN 600
           WADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKY SMDEIIRECN
Sbjct: 541 WADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYYSMDEIIRECN 600

Query: 601 SMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE 632
           SMAGQLD+WKLLDDAHDLVVTLHEKIETSFNE
Sbjct: 601 SMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE 606

BLAST of MC10g0030 vs. ExPASy TrEMBL
Match: A0A6J1G038 (small G protein signaling modulator 2-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111449473 PE=4 SV=1)

HSP 1 Score: 999 bits (2584), Expect = 0.0
Identity = 553/652 (84.82%), Postives = 575/652 (88.19%), Query Frame = 0

Query: 1   MIASGTDIAPSFGVGGGLVSRMMAGARPNAAVVAFTALAGLAVVLVLFYSASRALKRTQT 60
           MIASGTDIA SFGVGGG VSRMMAGARPNAAV A TALAGLAVV+VLFYSASRALK+T T
Sbjct: 1   MIASGTDIARSFGVGGGFVSRMMAGARPNAAV-AVTALAGLAVVVVLFYSASRALKQTPT 60

Query: 61  PSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSPASSSSSDRG 120
           PSSPDPNSNSPPSSSS SSASSS            WVHLRSVLFVVTSSSPASSSSSDRG
Sbjct: 61  PSSPDPNSNSPPSSSSLSSASSS------------WVHLRSVLFVVTSSSPASSSSSDRG 120

Query: 121 RLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLG 180
           RLKSPWSRKKRK+AL+PQQWRSL+TPDGKLRDGG+KFLKKVRSGGVDPSIRAEVWPFLLG
Sbjct: 121 RLKSPWSRKKRKHALSPQQWRSLYTPDGKLRDGGLKFLKKVRSGGVDPSIRAEVWPFLLG 180

Query: 181 VYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKE-------R 240
           VYDL+SSEEERDAVR+QKRKEYERLRKQCR LLK GDES KLNDDDMN +KE        
Sbjct: 181 VYDLTSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDESIKLNDDDMNCHKEGVIQHAAH 240

Query: 241 DENIKLN---------------DGETNY-NKEGDNQYVAHGDDCPSLEEVVSARESISSD 300
            ++  +N               D   N  NKEGDNQ+VAHGDD PSL +V+SARESISSD
Sbjct: 241 GDDSSMNCCNKEGVIQHAAHGDDSSMNCCNKEGDNQHVAHGDDSPSLGDVISARESISSD 300

Query: 301 ERGSNFRNLDGTSGVFLEEDDSSRRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGRE 360
           ERG N       SGV LEEDDSSR MT+ADAS  NTESSDSDSS DPEVS TFPSSDGRE
Sbjct: 301 ERGLN-------SGVLLEEDDSSRGMTNADASVLNTESSDSDSSGDPEVSHTFPSSDGRE 360

Query: 361 DNDPDFTSKNSSPSVAEVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDK 420
           DNDPDFTSKN SP V EVTSKFRSNEDFTTWQRIIRLDAVRAN+EWIAYAPSQAAVSDDK
Sbjct: 361 DNDPDFTSKNLSPLV-EVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDK 420

Query: 421 ARHSAEVVGLKDYDHLEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITE 480
           ARH AEVVGLKDYDHLE SRIFHA+RLVTILEAYALYDPEIGYCQGMSDLLSPIITVITE
Sbjct: 421 ARHLAEVVGLKDYDHLESSRIFHASRLVTILEAYALYDPEIGYCQGMSDLLSPIITVITE 480

Query: 481 DQEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVY 540
           D EAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRII+FKDSHLYRHL+ L+AEDCFFVY
Sbjct: 481 DHEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIRFKDSHLYRHLQNLEAEDCFFVY 540

Query: 541 RMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAAS 600
           RMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAAS
Sbjct: 541 RMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAAS 600

Query: 601 VLQKRKLIIEKYNSMDEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKIETS 629
           VLQKRKLIIEKYNSMDEIIRECNSMAGQLD+WKLLD AHDLVVTLHEKIETS
Sbjct: 601 VLQKRKLIIEKYNSMDEIIRECNSMAGQLDVWKLLDGAHDLVVTLHEKIETS 631

BLAST of MC10g0030 vs. ExPASy TrEMBL
Match: A0A6J1EAL1 (GTPase-activating protein GYP7 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430845 PE=4 SV=1)

HSP 1 Score: 944 bits (2441), Expect = 0.0
Identity = 505/580 (87.07%), Postives = 525/580 (90.52%), Query Frame = 0

Query: 53  RALKRTQTPSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSPA 112
           RALKRT T SSPDPNSNSPPSSSS        PSS+ SS+SSSWVHLRSVLFVVTSSSPA
Sbjct: 2   RALKRTPTSSSPDPNSNSPPSSSS--------PSSALSSASSSWVHLRSVLFVVTSSSPA 61

Query: 113 SSSSSDRGRLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRA 172
           SSSSSDRGRLKSPWSRKKRK+AL+PQQWRSLFTPDG+LRDGGIKFLKKVRSGGVDPSIRA
Sbjct: 62  SSSSSDRGRLKSPWSRKKRKHALSPQQWRSLFTPDGRLRDGGIKFLKKVRSGGVDPSIRA 121

Query: 173 EVWPFLLGVYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKE 232
           EVWPFLLG YDLSSSEEERDAVR+QKRKEYERLRKQCR LLK GD   KLNDD+MN    
Sbjct: 122 EVWPFLLGFYDLSSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDGCIKLNDDEMN---- 181

Query: 233 RDENIKLNDGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGV 292
                        +NK GD Q V+HGDD PSLE+V SARESISSDERG+NFR LDGTS V
Sbjct: 182 -------------FNKVGDAQLVSHGDDSPSLEDVGSARESISSDERGTNFRYLDGTSEV 241

Query: 293 FLEEDDSSRRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSPSV 352
            LEEDDSSR+MT+AD S  NTESSDSDSSEDPEVSQTFPSSDGREDNDP FTSKNSSP V
Sbjct: 242 LLEEDDSSRQMTNADVSILNTESSDSDSSEDPEVSQTFPSSDGREDNDPVFTSKNSSPLV 301

Query: 353 AEVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDYDH 412
            EVTSKFR NEDFTTWQRIIRLDAVRAN+EWIAYAPS AAVSDDKARHSAEVVGLKDYDH
Sbjct: 302 TEVTSKFRGNEDFTTWQRIIRLDAVRANAEWIAYAPSLAAVSDDKARHSAEVVGLKDYDH 361

Query: 413 LEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMRKA 472
           LE  RIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED EAFWCFVGFMRKA
Sbjct: 362 LESCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKA 421

Query: 473 RHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTFEQ 532
           RHNFRLDEVGIRKQLN+VS+II+FKDSHLYRHL+ L+AEDCFFVYRMVVVLFRRELTFEQ
Sbjct: 422 RHNFRLDEVGIRKQLNVVSKIIRFKDSHLYRHLQDLEAEDCFFVYRMVVVLFRRELTFEQ 481

Query: 533 TLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSM 592
           TLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKY SM
Sbjct: 482 TLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYYSM 541

Query: 593 DEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE 632
           DEIIRECNSMAGQLD+WKLLDDAHDLVVTLHEKIETSFNE
Sbjct: 542 DEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE 556

BLAST of MC10g0030 vs. TAIR 10
Match: AT3G49350.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 649.4 bits (1674), Expect = 2.9e-186
Identity = 372/581 (64.03%), Postives = 437/581 (75.22%), Query Frame = 0

Query: 53  RALKRTQTPSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSPA 112
           +AL+R QT             +SSSS+ SS   S  SSSSSSSW+ +RS LFVV SSSPA
Sbjct: 2   KALRRIQT-------------TSSSSNPSSPLSSPPSSSSSSSWIQIRSALFVVASSSPA 61

Query: 113 SSSSSDRGRLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRA 172
             S SDR RLKSPWSR+KRK  L PQQW+  FTPDG+LR+GG+  LKKVRS G++PSIR 
Sbjct: 62  --SCSDRPRLKSPWSRRKRKRPLRPQQWKRFFTPDGRLRNGGVDLLKKVRSRGIEPSIRL 121

Query: 173 EVWPFLLGVYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNKE 232
           EVWPFLLG+Y  +SS+EER  +R ++RKEYERLR+QC+ L K             ++N  
Sbjct: 122 EVWPFLLGLYGFNSSKEERVTIRNRRRKEYERLRRQCKRLQK-------------HNNGT 181

Query: 233 RDENIKLNDGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRNLDGTSGV 292
           R    KL +G      E D   V   D   S +EVVSARES+SSDE  +           
Sbjct: 182 R----KLYNGSETIQDEYDWPRVQDTDSSCS-DEVVSARESLSSDEDITEDIGYMSEVSC 241

Query: 293 FLEEDD--SSRRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNSSP 352
            +E DD  SSRR+T+A  S  N+ESSDSDSS++ EV Q F SS    D +  + + +S P
Sbjct: 242 TVERDDGSSSRRITNATISTLNSESSDSDSSDESEVVQVFQSS-ATPDVNSTYPASSSIP 301

Query: 353 SVAEVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLKDY 412
                    R+ EDF TWQRIIRLDAVRA+SEW +Y+PSQA +S+D+A  +AE VGLKDY
Sbjct: 302 ---------RTEEDFVTWQRIIRLDAVRADSEWTSYSPSQAVISEDRACRAAEAVGLKDY 361

Query: 413 DHLEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGFMR 472
           +HLEP +IF AARLV +LEAYALYDP+IGYCQGMSDLLSPI++VI +D E FWCFVGFM+
Sbjct: 362 NHLEPYKIFQAARLVAVLEAYALYDPDIGYCQGMSDLLSPILSVIPDDHEVFWCFVGFMK 421

Query: 473 KARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRRELTF 532
           KARHNFRLDEVGIR+QLNIVS+IIK KDS LYRHLEKLQAEDCFFVYRMVVV+FRRELT 
Sbjct: 422 KARHNFRLDEVGIRRQLNIVSKIIKSKDSQLYRHLEKLQAEDCFFVYRMVVVMFRRELTL 481

Query: 533 EQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYN 592
           +QTLCLWEVMWADQAAIRAG+GKSAWSRIRQRAPPT+DL+LYAIAASVLQ+RK IIE+YN
Sbjct: 482 DQTLCLWEVMWADQAAIRAGMGKSAWSRIRQRAPPTDDLVLYAIAASVLQRRKRIIERYN 539

Query: 593 SMDEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKIETSFN 632
           SMDEI+REC SMAGQLD+WKLLDDAHDLVVTLH KIE SF+
Sbjct: 542 SMDEILRECQSMAGQLDVWKLLDDAHDLVVTLHTKIEHSFS 539

BLAST of MC10g0030 vs. TAIR 10
Match: AT5G53570.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 616.7 bits (1589), Expect = 2.1e-176
Identity = 365/593 (61.55%), Postives = 437/593 (73.69%), Query Frame = 0

Query: 49  YSASRALKRTQTPSSPDPNSNSPPSSSSSSSASSSAPS---SSSSSSSSSWVHLRSVLFV 108
           Y+++ +   + + S P  +S+S PSSSSSS  SS++ S   S+SSSSSSSW+HLRSVLFV
Sbjct: 8   YTSTSSGNSSSSSSLPSSSSSSLPSSSSSSPPSSNSNSYSNSNSSSSSSSWIHLRSVLFV 67

Query: 109 VTSSSPASSSSSDRGRLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGG 168
              SSP+S +SSDR R KSPWSR+KRK ALTP QWRSLFTP+GKLRDGG+ FLKKVRS G
Sbjct: 68  ANLSSPSSVTSSDR-RRKSPWSRRKRKWALTPHQWRSLFTPEGKLRDGGVGFLKKVRSRG 127

Query: 169 VDPSIRAEVWPFLLGVYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDD 228
           VDPSIRAEVW FLLGVYDL+S+ EER+AV+ QKRKEYE+L+++C+ LLK G+ ST     
Sbjct: 128 VDPSIRAEVWLFLLGVYDLNSTSEEREAVKTQKRKEYEKLQRRCQMLLKCGNGST----- 187

Query: 229 DMNSNKERDENIKLNDGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRN 288
                                    DN                   E + SDE  S    
Sbjct: 188 -------------------------DN------------------LEELPSDEANSQCVR 247

Query: 289 LDGTSGVFLEEDDSSRRMTSAD-ASAPNTESSDSDSSEDPE---VSQTFPSSDGR---ED 348
                  F+++   +  MTS D  SA NT+SSD+DS ED E   +  +F  SD +   ED
Sbjct: 248 -------FVDDYKITGPMTSQDVVSALNTDSSDTDSCEDNEDVLLLSSFAHSDEKKPEED 307

Query: 349 NDPDFTSKNSSPSVA---EVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSD 408
           N  + + +NSS  VA   EV  +   +EDF+TWQRIIRLDA+RA+SEW  Y+P   A+++
Sbjct: 308 NSNNNSEENSSLLVAAASEVQVEVAVHEDFSTWQRIIRLDALRADSEWANYSPYSTAITE 367

Query: 409 DKARHSAEVVGLKDYDHLEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVI 468
            KAR  AE VGLKDYDHLE  R++HAARLV ILEAYA+YDPEIGYCQGMSDLLSPI+ VI
Sbjct: 368 SKARRLAESVGLKDYDHLESCRLYHAARLVAILEAYAMYDPEIGYCQGMSDLLSPILAVI 427

Query: 469 TEDQEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFF 528
           +ED EAFWCFVGFM+KARHNFRLDE GI++QL+IVS+IIK KDS LY+HLE LQAEDC F
Sbjct: 428 SEDHEAFWCFVGFMKKARHNFRLDEAGIQRQLSIVSKIIKNKDSQLYKHLENLQAEDCSF 487

Query: 529 VYRMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIA 588
           VYRMV+V+FRREL+FEQTLCLWEVMWADQAAIRAGVGKS WSRIRQ+APPT+DLLLYAIA
Sbjct: 488 VYRMVLVMFRRELSFEQTLCLWEVMWADQAAIRAGVGKSPWSRIRQQAPPTDDLLLYAIA 543

Query: 589 ASVLQKRKLIIEKYNSMDEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKIET 629
           A VL +RKLII+KY+SMDEI+ ECNSMAGQL++WKLLDDAH LVVTLH+KIET
Sbjct: 548 ALVL-RRKLIIQKYSSMDEIVEECNSMAGQLNVWKLLDDAHHLVVTLHDKIET 543

BLAST of MC10g0030 vs. TAIR 10
Match: AT5G53570.2 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 616.7 bits (1589), Expect = 2.1e-176
Identity = 365/593 (61.55%), Postives = 437/593 (73.69%), Query Frame = 0

Query: 49  YSASRALKRTQTPSSPDPNSNSPPSSSSSSSASSSAPS---SSSSSSSSSWVHLRSVLFV 108
           Y+++ +   + + S P  +S+S PSSSSSS  SS++ S   S+SSSSSSSW+HLRSVLFV
Sbjct: 35  YTSTSSGNSSSSSSLPSSSSSSLPSSSSSSPPSSNSNSYSNSNSSSSSSSWIHLRSVLFV 94

Query: 109 VTSSSPASSSSSDRGRLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGG 168
              SSP+S +SSDR R KSPWSR+KRK ALTP QWRSLFTP+GKLRDGG+ FLKKVRS G
Sbjct: 95  ANLSSPSSVTSSDR-RRKSPWSRRKRKWALTPHQWRSLFTPEGKLRDGGVGFLKKVRSRG 154

Query: 169 VDPSIRAEVWPFLLGVYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDD 228
           VDPSIRAEVW FLLGVYDL+S+ EER+AV+ QKRKEYE+L+++C+ LLK G+ ST     
Sbjct: 155 VDPSIRAEVWLFLLGVYDLNSTSEEREAVKTQKRKEYEKLQRRCQMLLKCGNGST----- 214

Query: 229 DMNSNKERDENIKLNDGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERGSNFRN 288
                                    DN                   E + SDE  S    
Sbjct: 215 -------------------------DN------------------LEELPSDEANSQCVR 274

Query: 289 LDGTSGVFLEEDDSSRRMTSAD-ASAPNTESSDSDSSEDPE---VSQTFPSSDGR---ED 348
                  F+++   +  MTS D  SA NT+SSD+DS ED E   +  +F  SD +   ED
Sbjct: 275 -------FVDDYKITGPMTSQDVVSALNTDSSDTDSCEDNEDVLLLSSFAHSDEKKPEED 334

Query: 349 NDPDFTSKNSSPSVA---EVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSD 408
           N  + + +NSS  VA   EV  +   +EDF+TWQRIIRLDA+RA+SEW  Y+P   A+++
Sbjct: 335 NSNNNSEENSSLLVAAASEVQVEVAVHEDFSTWQRIIRLDALRADSEWANYSPYSTAITE 394

Query: 409 DKARHSAEVVGLKDYDHLEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVI 468
            KAR  AE VGLKDYDHLE  R++HAARLV ILEAYA+YDPEIGYCQGMSDLLSPI+ VI
Sbjct: 395 SKARRLAESVGLKDYDHLESCRLYHAARLVAILEAYAMYDPEIGYCQGMSDLLSPILAVI 454

Query: 469 TEDQEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFF 528
           +ED EAFWCFVGFM+KARHNFRLDE GI++QL+IVS+IIK KDS LY+HLE LQAEDC F
Sbjct: 455 SEDHEAFWCFVGFMKKARHNFRLDEAGIQRQLSIVSKIIKNKDSQLYKHLENLQAEDCSF 514

Query: 529 VYRMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIA 588
           VYRMV+V+FRREL+FEQTLCLWEVMWADQAAIRAGVGKS WSRIRQ+APPT+DLLLYAIA
Sbjct: 515 VYRMVLVMFRRELSFEQTLCLWEVMWADQAAIRAGVGKSPWSRIRQQAPPTDDLLLYAIA 570

Query: 589 ASVLQKRKLIIEKYNSMDEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKIET 629
           A VL +RKLII+KY+SMDEI+ ECNSMAGQL++WKLLDDAH LVVTLH+KIET
Sbjct: 575 ALVL-RRKLIIQKYSSMDEIVEECNSMAGQLNVWKLLDDAHHLVVTLHDKIET 570

BLAST of MC10g0030 vs. TAIR 10
Match: AT5G24390.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 597.4 bits (1539), Expect = 1.3e-170
Identity = 325/542 (59.96%), Postives = 407/542 (75.09%), Query Frame = 0

Query: 90  SSSSSSWVHLRSVLFVVTSSSPAS-SSSSDRGRLKSPWSRKKRKNALTPQQWRSLFTPDG 149
           SS SS W HLRS   +VTSSSPAS SSSSD  RLKSPWSR+K K  LT ++WR  FTP+G
Sbjct: 17  SSPSSPWTHLRSAFVLVTSSSPASCSSSSDPHRLKSPWSRRKGKKPLTLRRWRRCFTPEG 76

Query: 150 KLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYDLSSSEEERDAVRLQKRKEYERLRKQ 209
           ++R+GG+  LKKVR+ G+DPSIR+EVWPFLLGV D +SSEEER A R  +RK YERLR+Q
Sbjct: 77  RIRNGGVDLLKKVRTRGIDPSIRSEVWPFLLGVCDFNSSEEERGATRTWRRKVYERLRRQ 136

Query: 210 CRGLLKSGDESTKLNDDDMNSNKERDENIKLNDGETNYNKEGDNQYVAHGDDCPSLEEVV 269
           C+ L K    + KLN              K+N  +T  + E D+  +A   +    ++  
Sbjct: 137 CKRLQKQNSGTFKLN--------------KIN--KTTQDDEHDSWSLAQDSESSCSDDAC 196

Query: 270 SARESISSDERGSNFRNLDGTSGVFLEEDDSSRRMTSADASAPNTESSDSDSSEDPEVSQ 329
           S  ES+ SD+  +            L+ D +  R         N+ESSDSDSS++ +  Q
Sbjct: 197 SVHESLISDKDNTEDIGYMSDVSCTLDRDYNGSRHL-------NSESSDSDSSDENDYVQ 256

Query: 330 TFPSSDGREDNDPDFTSKNSSPSVAEVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAP 389
             PS +G+++N       ++SPS   ++   R+ EDF TWQRIIRLDA+RA++EW  Y+ 
Sbjct: 257 VAPSCEGKDEN-------STSPSSIYIS---RTKEDFVTWQRIIRLDALRADTEWTPYSQ 316

Query: 390 SQAAVSDDKARHSAEVVGLKDYDHLEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLL 449
           SQA VS+++AR +AE VGLKDY +LEPS+IFHAARLV +LEAYAL+DPEIGYCQGMSDLL
Sbjct: 317 SQALVSENRARRAAEAVGLKDYSYLEPSKIFHAARLVAVLEAYALHDPEIGYCQGMSDLL 376

Query: 450 SPIITVITEDQEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKL 509
           SPI++VI +D EAFWCFVGFM+KAR NFR+DEVGI +QLNIVS+IIK KDS LY+HLEK+
Sbjct: 377 SPILSVIPDDYEAFWCFVGFMKKARQNFRVDEVGITRQLNIVSKIIKSKDSQLYKHLEKV 436

Query: 510 QAEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWS-RIRQRAPPTE 569
           +AEDCFFVYRMV+V+FRRELT EQTL LWEV+WADQAAIRAG+GKS+WS RI+QRAPPTE
Sbjct: 437 KAEDCFFVYRMVLVMFRRELTLEQTLHLWEVIWADQAAIRAGMGKSSWSRRIKQRAPPTE 496

Query: 570 DLLLYAIAASVLQKRKLIIEKYNSMDEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKIE 629
           DLLLY +AASVLQ+RK+IIEKY+SM+EI+REC++M G+LD+WKLLDDAHDL+VTLH KIE
Sbjct: 497 DLLLYVVAASVLQRRKVIIEKYSSMEEILRECHNMVGKLDVWKLLDDAHDLIVTLHAKIE 525

BLAST of MC10g0030 vs. TAIR 10
Match: AT5G41940.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 561.2 bits (1445), Expect = 1.0e-159
Identity = 311/526 (59.13%), Postives = 377/526 (71.67%), Query Frame = 0

Query: 101 SVLFVVTSSSPASSSSSDRGRLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKK 160
           +V   V + + A +  S RG + SPWS ++RK  L P+QW + FT +G+L DGG+KFLKK
Sbjct: 55  AVAVTVMAVAVAFTVYSRRGSIGSPWSLRRRKRVLQPKQWNAFFTEEGRLSDGGVKFLKK 114

Query: 161 VRSGGVDPSIRAEVWPFLLGVYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDEST 220
           VRSGGV PSIR EVWPFLLGVYDL S++EERD++R  K  EYE LR+QCR +        
Sbjct: 115 VRSGGVHPSIRPEVWPFLLGVYDLKSNKEERDSIRQLKLTEYENLRRQCREI-------- 174

Query: 221 KLNDDDMNSNKERDENIKLNDGETNYNKEGDNQYVAHGDDCPSLEEVVSARESISSDERG 280
                      ER EN+  +  ET  +   ++  V    D   +EEV S+  +I+ DE  
Sbjct: 175 ----------HERYENV-CDSKETAQSSNTEDSQVLESHD---IEEVESSTRTITVDES- 234

Query: 281 SNFRNLDGTSGVFLEEDDSSRRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDND 340
                L+  S +  +E+     +T+ DA+      SDS + E+ E S    + +    N 
Sbjct: 235 ---EKLNSES-IMQDENCEKSDVTTEDAAG---NDSDSTNPEETETSPFLANEEVESHNT 294

Query: 341 PDFTSKNSSPSVAEVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARH 400
            +   + SSPS ++  S+    +  +TWQRIIRLDAVRAN EW+ Y+P+QAAVS+ KAR 
Sbjct: 295 VNQEKEISSPS-SKPKSQAADEDFMSTWQRIIRLDAVRANDEWVPYSPTQAAVSETKARG 354

Query: 401 SAEVVGLKDYDHLEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQE 460
            A  VGL DYDHLEP RIFHAARLV ILEAYA+YDPEIGYCQGMSDLLSP+I V+ +D  
Sbjct: 355 IAIQVGLNDYDHLEPCRIFHAARLVGILEAYAVYDPEIGYCQGMSDLLSPLIAVMEDDVL 414

Query: 461 AFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMV 520
           AFWCFVGFM KARHNFRLDEVGIR+QL++VS+IIKFKD HLYRHLE L+AEDCFFVYRMV
Sbjct: 415 AFWCFVGFMSKARHNFRLDEVGIRRQLSMVSKIIKFKDIHLYRHLENLEAEDCFFVYRMV 474

Query: 521 VVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQ 580
           VVLFRRELTFEQTLCLWEVMWADQAAIR G+ K+ W RIR RAPPTEDLLLYAIAASVLQ
Sbjct: 475 VVLFRRELTFEQTLCLWEVMWADQAAIRTGIAKATWGRIRLRAPPTEDLLLYAIAASVLQ 534

Query: 581 KRKLIIEKYNSMDEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKI 627
           +RK IIEKY+ MDEI++ECNSMAG LD+WKLLDDAHDLVV LH+KI
Sbjct: 535 RRKTIIEKYSGMDEIMKECNSMAGHLDVWKLLDDAHDLVVNLHDKI 549