MC09g_new0479 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g_new0479
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPectin lyase-like superfamily protein
LocationMC09: 22886198 .. 22889433 (-)
RNA-Seq ExpressionMC09g_new0479
SyntenyMC09g_new0479
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGAATTTTTTTCCCCCACATTTTTAATGTACAGAATTTAACGGTATGCAACTGTATTTTACCATGCATAGAAGAAACTTGAGAGTTGAGCTGCCAAGGAAGAAGAAAATGTGAAGTGAGCTCACAACAAAGAGAGAGAGCGATGGGGGAGACACAAAACAAAATGCATCAATGGCGGTGTTGAATTTGGTGTGCTGGTTTTAGAGTAAAGCAAAGAAACTTCAAATATTTTCTCTTCTACTTTACGTGTAGGCATGTTTGAAAATGGCGACCTCCTCTTAATCTGCTTTCAAGCAAAGCCACAGCCCACTAAAATGGCTTCTTTATTTCCCCTTATAAATTCTAACCTCTTTCATTGGATCATTGCATTAACACAAATCACTCCAGTAATCAATCTCTTCTACCAAGTCGACTCGAAATGGATAAATTCTTCGTTCTTTCTCTTCTCGGTATGCTGATAGTTGCCTGTGGAGTTTCGGGGAGTATCGAGCCCGATGGCGACATTGACATGATTAGAGAACTTGAAAATTTCGACATTGGAGGAGAGGATGACGATGTCGTGGACGCATCTGCCGTCCCGGTGTGGAGAAGCGAGCGCGGGAGCAAGGTTCTGATGAATGTGGATAGTTTCAATGCTGTTGGAGATGGAGTTTCAGATGATACAATGGTACATAGACATTTCTGTTTGGAAATATGGATTTTTGCAGGGAGAATAGTGAATCAAAGTGTTCATATTTTGTAATCAGGCTTTCAGAAAGGCATGGGAGACTGCCTGCAGCACTTCAAAATCAGTTCTTTTGGTGCCAAAAGGAGGGACATATCTGGTGAATGCAACCACTTTTAAAGGGCCATGTAAAGATGACCTAGTAATCCAGGTAAGCCATAAGATTATGATGGTTTTCCTTTTTGCAAATAAACAAGTTGAGAAGATCATGGTTACAAAAACAGAGTTAAACTTTGCAGATTGAAGGAACAATAGTTGCACCAGATGAGCCTGAGAAATGGGATCCAAAATTTCAGAGACAATGGCTAGAATTTACCAAACTAAATGCAGTTACCTTTCAAGGGGATGGAGTTATCGACGGCTCGGGCGACAAATGGTGGGCGGAATCCTGCAAAAAGAACAAGTCAAGGGTAAATTTTTTAATTGTCTCTGAGCTTGATTCCTTGGCTATGTGATTGAAATGGTATTCACTCTGGTTTTCTGGTTGTTTTTCTTTTCAGCCCTGCAAAGGAGCACCAACAGTAAGGACTAAACTACTCTCCTCTGAATGAAAACCATGAGTTTTCATGTGACATTCTGATTTGAGGGCTTTCTTGCAGGCATTAACTATTGATTCAAGTTCAAATATAAGAGTAAAAGGCCTTACTATTCAGAACAGCCAACAGATGCATTTCACCATTGCACGGTCTGAATCTGTTCGGGTTACGGAGGTAAGAGTCTCGGCTCCCGGAGATAGCCCCAATACGGACGGGATCCATATCACTCAATCAACTAATGTTGTAATCCAAAACTGCAAGATTTCAACAGGTTCTCATTCTGACCTTCATCAAGTTATCATTAAACACACCCATTTCCTAAACTGGCTGAAAAGGGTCTTGATTTAGTAATGGTTGTGCAATTCATGCAGGTGATGACTGCATCTCCATTGTCAATGCTAGTTCTGGCATCAAGATGAAAGGAATATCATGTGGACCGGGGCATGGGATCAGGTAACTGCAGATGTTTTCATTTCACTCGGCATCGTTTGATTTGTTTCGAGTACCAAAACCAGATGAATGTCTTAGAATTCTTTCCTTTTGTTTGTTATGAAACTGTGTTCCTTAGCATTGGAAGTTTAGGGAAGGACAACTCCACTGGCATTGTAACAAAGGTGGTGTTAGACACAGCCTTCCTCAGAGAGACAACAAATGGTGTTAGAATCAAGACTTGGCAGGTTAGTATCACAGCTTCAATCACCATTTGAATATGAATTTTACATGGTTGATTATATAAAACACGTAACGGGAAAATCGCTCTATTCGTGTGGTGTAGGGGGGTTCTGGATATGTTCGTAGCGTGCGATTCGAAAATGTGAGAATGGAAGATGTTGAGAATCCCATCATCATTGATCAATTCTATTGTGACTCTCCTACTTCCTGTGAAACTCAGGTACACAATCCTCAAAATCCATATTTAGCTTCTTCTAATCAAACAGTGACATTAAATATAAGATCATATTGAGATAGTAAGACAAATTTTGTTGATATCAATCATTAATCTCAATGCCTTTTGCTTTTGTTAGACATCAGCTGTGAAAATAAGCCAAATAATGTATCGGAACATAAGCGGAACCACAACGAGCAAGAACGCGATGAAATTCGCTTGTAGCGACAGCGTCCCGTGTAGTAACATAGTTCTAAGCAACGTCAACTTGGAGAAGACGGATGGAACAGTGGAGACCTATTGCCACTCTGCCCAAGGTTTTGGATACGGCGTCGTGCACCCCTCAGCAGACTGCCTAACTTCCAGCGACAAGTATTCCGCAGCCTCGGATCAGACCGAAGTTCCCGAGCTCGTGGAACTGCACCTCCCAACTGAACACATTGTTCACACTGAGCTCTGACCTTGTTGTCCTGTCTCTCTATACTAAACTACAAAGGGGATCATATTTTGGTAGAGCTTGGTTTTCAGCCGACCAACTCAGGAAATATTTGTACTGTAGCATATATTTACATAGCATATACAATTACACCTACCCTTTTGGAATCTAAATAGAAATATAGACTGCTAAGTATATGATTGTACTATACTATTACTAGAAGAAAAATATACATATTTTGAGCATATAGTAAAGTTTGTTAGCACTAAAGTTCATATCATATGGTTGATGGCTCTCTGAAGATATAATATACACAAAAAGGGGAAGATTGAGGAACAAGGACCACTTATCTTGGAGTACAGTGGACGCCCCGCAAACAGATCAAACTGGGGCTTAAAAACGCGCCACTTTCTGGTAGAATTTCAACTTCATGAAAGCGAAAACTGGCGTATAGTAGAAGTGTCATGGAACAAGACAAAAGTGGTCCAATTGTATCAAAACGGCACTGATTCCACTAACTGATGGTCAATCAAACAGAAAAAACGCACCCCAACAACCCCATAAAACTAGATGGAAATAAAAAACTAATGGTATCCAATGCTAACTTGGAAGGGCAGTGCCAGCTTACAAGCTATACAGGAGAAGATAATAAAG

mRNA sequence

ATGCATTTCACCATTGCACGGTCTGAATCTGTTCGGGTTACGGAGGTAAGAGTCTCGGCTCCCGGAGATAGCCCCAATACGGACGGGATCCATATCACTCAATCAACTAATGTTGTAATCCAAAACTGCAAGATTTCAACAGGTGATGACTGCATCTCCATTGTCAATGCTAGTTCTGGCATCAAGATGAAAGGAATATCATGTGGACCGGGGCATGGGATCAGCATTGGAAGTTTAGGGAAGGACAACTCCACTGGCATTGTAACAAAGGTGGTGTTAGACACAGCCTTCCTCAGAGAGACAACAAATGGTGTTAGAATCAAGACTTGGCAGGGGGGTTCTGGATATGTTCGTAGCGTGCGATTCGAAAATGTGAGAATGGAAGATGTTGAGAATCCCATCATCATTGATCAATTCTATTGTGACTCTCCTACTTCCTGTGAAACTCAGACATCAGCTGTGAAAATAAGCCAAATAATGTATCGGAACATAAGCGGAACCACAACGAGCAAGAACGCGATGAAATTCGCTTGTAGCGACAGCGTCCCGTGTAGTAACATAGTTCTAAGCAACGTCAACTTGGAGAAGACGGATGGAACAGTGGAGACCTATTGCCACTCTGCCCAAGGTTTTGGATACGGCGTCGTGCACCCCTCAGCAGACTGCCTAACTTCCAGCGACAAGTATTCCGCAGCCTCGGATCAGACCGAAGTTCCCGAGCTCGTGGAACTGCACCTCCCAACTGAACACATTGTTCACACTGAGCTCTGA

Coding sequence (CDS)

ATGCATTTCACCATTGCACGGTCTGAATCTGTTCGGGTTACGGAGGTAAGAGTCTCGGCTCCCGGAGATAGCCCCAATACGGACGGGATCCATATCACTCAATCAACTAATGTTGTAATCCAAAACTGCAAGATTTCAACAGGTGATGACTGCATCTCCATTGTCAATGCTAGTTCTGGCATCAAGATGAAAGGAATATCATGTGGACCGGGGCATGGGATCAGCATTGGAAGTTTAGGGAAGGACAACTCCACTGGCATTGTAACAAAGGTGGTGTTAGACACAGCCTTCCTCAGAGAGACAACAAATGGTGTTAGAATCAAGACTTGGCAGGGGGGTTCTGGATATGTTCGTAGCGTGCGATTCGAAAATGTGAGAATGGAAGATGTTGAGAATCCCATCATCATTGATCAATTCTATTGTGACTCTCCTACTTCCTGTGAAACTCAGACATCAGCTGTGAAAATAAGCCAAATAATGTATCGGAACATAAGCGGAACCACAACGAGCAAGAACGCGATGAAATTCGCTTGTAGCGACAGCGTCCCGTGTAGTAACATAGTTCTAAGCAACGTCAACTTGGAGAAGACGGATGGAACAGTGGAGACCTATTGCCACTCTGCCCAAGGTTTTGGATACGGCGTCGTGCACCCCTCAGCAGACTGCCTAACTTCCAGCGACAAGTATTCCGCAGCCTCGGATCAGACCGAAGTTCCCGAGCTCGTGGAACTGCACCTCCCAACTGAACACATTGTTCACACTGAGCTCTGA

Protein sequence

MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSGIKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVRFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDSVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPELVELHLPTEHIVHTEL
Homology
[Close]
BLAST of MC09g_new0479 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.8e-60
Identity = 118/240 (49.17%), Postives = 156/240 (65.00%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           MH  +     V ++ ++V AP  SPNTDGIHI+ S  +VI N  +STGDDCISIV  S+ 
Sbjct: 201 MHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQ 260

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           I +  I CGPGHGISIGSLGK  S   V  + +DTA + +T NGVRIKTWQGGSG V  +
Sbjct: 261 ISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKI 320

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
            F N++M +V NPIIIDQ+YCDS   C  QTSA+ I  I + ++ GT+ SK A+K +CSD
Sbjct: 321 IFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHVRGTSASKEAIKISCSD 380

Query: 181 SVPCSNIVLSNVNLEKT--DGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEV 239
           S PC NI+L +++LE +  DG  E++C  A G   G V+P   CL+    +   S Q+ +
Sbjct: 381 SSPCRNILLQDIDLEPSNGDGFTESFCWEAYGSSSGQVYP-PPCLSDDTSFLEQSVQSGI 439

[Close]
BLAST of MC09g_new0479 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 3.6e-55
Identity = 110/219 (50.23%), Postives = 148/219 (67.58%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           +H +     +V+ + + V+AP +SPNTDGIH+T + N+ I +C I TGDDCISIVN S  
Sbjct: 235 IHVSFDNCVNVQASNLMVTAPENSPNTDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRK 294

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           +++  I+CGPGHGISIGSLG  NS   V+ VV++ A L  TTNGVRIKTWQGGSG   ++
Sbjct: 295 VRVNDITCGPGHGISIGSLGYGNSEAHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNI 354

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
           +F+NV M +VENPIIIDQ YCD    C+ Q+SAV++  ++Y+NI GT  S  A+ F CS 
Sbjct: 355 KFQNVEMHNVENPIIIDQNYCDQDKPCQEQSSAVQVKNVVYQNIKGTCASNVAITFDCSK 414

Query: 181 SVPCSNIVLSNVNLEKTDG-TVETYCHSAQGFGYGVVHP 219
             PC  IVL +V+LE   G   +  C++ +    GVV P
Sbjct: 415 RFPCQGIVLEDVDLEIEGGAAAKALCNNVELSETGVVSP 453

[Close]
BLAST of MC09g_new0479 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.0e-54
Identity = 109/223 (48.88%), Postives = 146/223 (65.47%), Query Frame = 0 

Query: 2   HFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSGI 61
           H      + V++  +++ AP DSPNTDGI I  S    I+ C I TGDDC+++   SS I
Sbjct: 209 HLVFGECDGVKIQGIKIKAPRDSPNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTGSSNI 268

Query: 62  KMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSVR 121
            +K ++CGPGHG+SIGSLGK NS   V+ V LD A   +T NG+RIKTWQGGSG    + 
Sbjct: 269 TIKDLTCGPGHGMSIGSLGKGNSRSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLASHIT 328

Query: 122 FENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSDS 181
           +ENV M + ENPI+I+QFYC S  +C+ Q SAVKI  + ++NI GT+ +  A++  CSDS
Sbjct: 329 YENVEMINAENPILINQFYCTSAAACKNQRSAVKIQDVTFKNIHGTSATTAAIQLMCSDS 388

Query: 182 VPCSNIVLSNVNLEKTDGTVETYCH-SAQGFGYGVVHPSADCL 224
           VPCSNI LSNV L+ T G V T  + +A G+    ++PS   L
Sbjct: 389 VPCSNIKLSNVFLKLTSGKVATCVNKNANGYYTNPLNPSCKSL 431

[Close]
BLAST of MC09g_new0479 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 211.5 bits (537), Expect = 1.2e-53
Identity = 99/194 (51.03%), Postives = 141/194 (72.68%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           MH T    ++V+   + V++P DSPNTDGIH++ + N++IQ+  + TGDDCISIV+ S  
Sbjct: 217 MHLTFQDCKNVKALNLMVTSPADSPNTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSEN 276

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           ++  GI+CGPGHGISIGSLG+DNS   V+ VV++ A L  TTNGVRIKTWQGG G  +++
Sbjct: 277 VRATGITCGPGHGISIGSLGEDNSEAYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNI 336

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
            F+++ M++V NPIII+Q YCD   +C  Q SAV++S ++Y+NI GT++   A+KF CS 
Sbjct: 337 IFQDIIMKNVTNPIIINQDYCDRVEACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSK 396

Query: 181 SVPCSNIVLSNVNL 195
           ++PC  I + NV L
Sbjct: 397 NIPCRGISMQNVKL 410

[Close]
BLAST of MC09g_new0479 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 2.6e-53
Identity = 106/218 (48.62%), Postives = 147/218 (67.43%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           +  +I +   V V+ V ++APGDSPNTDGIHIT + N+ + N  I TGDDCISI + +  
Sbjct: 216 IQISIEKCNKVEVSNVEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQN 275

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           +++  ++CGPGHGISIGSLG DNS   V+ + +D A   E+ NGVRIKT+QGGSG  +++
Sbjct: 276 LQIFDLTCGPGHGISIGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQGGSGTAKNI 335

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
           +F+N+RME+V+NPIIIDQ YCD    CE Q SAV++  ++Y+NISGT+ +  A+   CS+
Sbjct: 336 KFQNIRMENVKNPIIIDQDYCDK-DKCEDQESAVQVKNVVYKNISGTSATDVAITLNCSE 395

Query: 181 SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHP 219
             PC  IVL NV ++   GT    C +A     G V P
Sbjct: 396 KYPCQGIVLENVKIK--GGTAS--CKNANVKNQGTVSP 428

[Close]
BLAST of MC09g_new0479 vs. NCBI nr
Match: XP_022151049.1 (probable polygalacturonase At1g80170 [Momordica charantia])

HSP 1 Score: 511 bits (1315), Expect = 3.38e-179
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG
Sbjct: 213 MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 272

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV
Sbjct: 273 IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 332

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
           RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD
Sbjct: 333 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 392

Query: 181 SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPE 240
           SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPE
Sbjct: 393 SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPE 452

Query: 241 LVELHLPTEHIVHTEL 256
           LVELHLPTEHIVHTEL
Sbjct: 453 LVELHLPTEHIVHTEL 468

[Close]
BLAST of MC09g_new0479 vs. NCBI nr
Match: XP_008446196.1 (PREDICTED: probable polygalacturonase At1g80170 [Cucumis melo])

HSP 1 Score: 473 bits (1218), Expect = 6.78e-164
Identity = 238/257 (92.61%), Postives = 247/257 (96.11%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           MHFTIARSE+VR+TEVRVSAPGDSPNTDGIHITQSTNVV+QN KISTGDDCISIVNASSG
Sbjct: 250 MHFTIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNSKISTGDDCISIVNASSG 309

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV
Sbjct: 310 IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 369

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
           RFENVRMEDVENPIIIDQFYCDSPT+CETQTSAVKISQIMYRNISGTTTSKNAMKFACSD
Sbjct: 370 RFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 429

Query: 181 SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPE 240
           SVPCSNI+LSNVNLEKTDGTVETYCH+AQGFGYG+VHPSADCLTSSD   AA DQT+V E
Sbjct: 430 SVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAALDQTQVLE 489

Query: 241 LVELHLPTE-HIVHTEL 256
           L  + LPTE HIVHTEL
Sbjct: 490 LETVQLPTEQHIVHTEL 506

[Close]
BLAST of MC09g_new0479 vs. NCBI nr
Match: TYK15614.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 476 bits (1224), Expect = 1.36e-163
Identity = 239/257 (93.00%), Postives = 248/257 (96.50%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           MHFTIARSE+VR+TEVRVSAPGDSPNTDGIHITQSTNVV+QN KISTGDDCISIVNASSG
Sbjct: 335 MHFTIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNSKISTGDDCISIVNASSG 394

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV
Sbjct: 395 IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 454

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
           RFENVRMEDVENPIIIDQFYCDSPT+CETQTSAVKISQIMYRNISGTTTSKNAMKFACSD
Sbjct: 455 RFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 514

Query: 181 SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPE 240
           SVPCSNI+LSNVNLEKTDGTVETYCH+AQGFGYG+VHPSADCLTSSD   AASDQT+V E
Sbjct: 515 SVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAASDQTQVLE 574

Query: 241 LVELHLPTE-HIVHTEL 256
           L  + LPTE HIVHTEL
Sbjct: 575 LETVQLPTEQHIVHTEL 591

[Close]
BLAST of MC09g_new0479 vs. NCBI nr
Match: KAA0034306.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 476 bits (1224), Expect = 1.41e-163
Identity = 239/257 (93.00%), Postives = 248/257 (96.50%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           MHFTIARSE+VR+TEVRVSAPGDSPNTDGIHITQSTNVV+QN KISTGDDCISIVNASSG
Sbjct: 336 MHFTIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNSKISTGDDCISIVNASSG 395

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV
Sbjct: 396 IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 455

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
           RFENVRMEDVENPIIIDQFYCDSPT+CETQTSAVKISQIMYRNISGTTTSKNAMKFACSD
Sbjct: 456 RFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 515

Query: 181 SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPE 240
           SVPCSNI+LSNVNLEKTDGTVETYCH+AQGFGYG+VHPSADCLTSSD   AASDQT+V E
Sbjct: 516 SVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAASDQTQVLE 575

Query: 241 LVELHLPTE-HIVHTEL 256
           L  + LPTE HIVHTEL
Sbjct: 576 LETVQLPTEQHIVHTEL 592

[Close]
BLAST of MC09g_new0479 vs. NCBI nr
Match: XP_011655619.1 (probable polygalacturonase At1g80170 isoform X1 [Cucumis sativus])

HSP 1 Score: 469 bits (1207), Expect = 9.10e-163
Identity = 235/257 (91.44%), Postives = 247/257 (96.11%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           MHFTIARSE+VR+T VRVSAPGDSPNTDGIHITQSTNVV+QNCKISTGDDCISIVNASSG
Sbjct: 213 MHFTIARSETVRITGVRVSAPGDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSG 272

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTA+LRETTNGVRIKTWQGGSGYVR+V
Sbjct: 273 IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAV 332

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
           RFENVRMEDVENPIIIDQFYCDSPT+CE+QTSAVKISQIMYRNISGTTTSKNAMKFACSD
Sbjct: 333 RFENVRMEDVENPIIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSD 392

Query: 181 SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPE 240
           SVPCSNI+LSNVNLEKTDGTVETYCH+AQGFGYGVVHPSADCLTSSD   A+SDQT+V E
Sbjct: 393 SVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLE 452

Query: 241 LVELHLPTE-HIVHTEL 256
           L  + L TE HIVHTEL
Sbjct: 453 LETVQLSTEQHIVHTEL 469

[Close]
BLAST of MC09g_new0479 vs. ExPASy TrEMBL
Match: A0A6J1DBU9 (probable polygalacturonase At1g80170 OS=Momordica charantia OX=3673 GN=LOC111019073 PE=3 SV=1)

HSP 1 Score: 511 bits (1315), Expect = 1.64e-179
Identity = 256/256 (100.00%), Postives = 256/256 (100.00%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG
Sbjct: 213 MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 272

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV
Sbjct: 273 IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 332

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
           RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD
Sbjct: 333 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 392

Query: 181 SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPE 240
           SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPE
Sbjct: 393 SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPE 452

Query: 241 LVELHLPTEHIVHTEL 256
           LVELHLPTEHIVHTEL
Sbjct: 453 LVELHLPTEHIVHTEL 468

[Close]
BLAST of MC09g_new0479 vs. ExPASy TrEMBL
Match: A0A1S3BF58 (probable polygalacturonase At1g80170 OS=Cucumis melo OX=3656 GN=LOC103489000 PE=3 SV=1)

HSP 1 Score: 473 bits (1218), Expect = 3.28e-164
Identity = 238/257 (92.61%), Postives = 247/257 (96.11%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           MHFTIARSE+VR+TEVRVSAPGDSPNTDGIHITQSTNVV+QN KISTGDDCISIVNASSG
Sbjct: 250 MHFTIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNSKISTGDDCISIVNASSG 309

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV
Sbjct: 310 IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 369

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
           RFENVRMEDVENPIIIDQFYCDSPT+CETQTSAVKISQIMYRNISGTTTSKNAMKFACSD
Sbjct: 370 RFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 429

Query: 181 SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPE 240
           SVPCSNI+LSNVNLEKTDGTVETYCH+AQGFGYG+VHPSADCLTSSD   AA DQT+V E
Sbjct: 430 SVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAALDQTQVLE 489

Query: 241 LVELHLPTE-HIVHTEL 256
           L  + LPTE HIVHTEL
Sbjct: 490 LETVQLPTEQHIVHTEL 506

[Close]
BLAST of MC09g_new0479 vs. ExPASy TrEMBL
Match: A0A5D3CXE4 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001110 PE=3 SV=1)

HSP 1 Score: 476 bits (1224), Expect = 6.60e-164
Identity = 239/257 (93.00%), Postives = 248/257 (96.50%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           MHFTIARSE+VR+TEVRVSAPGDSPNTDGIHITQSTNVV+QN KISTGDDCISIVNASSG
Sbjct: 335 MHFTIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNSKISTGDDCISIVNASSG 394

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV
Sbjct: 395 IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 454

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
           RFENVRMEDVENPIIIDQFYCDSPT+CETQTSAVKISQIMYRNISGTTTSKNAMKFACSD
Sbjct: 455 RFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 514

Query: 181 SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPE 240
           SVPCSNI+LSNVNLEKTDGTVETYCH+AQGFGYG+VHPSADCLTSSD   AASDQT+V E
Sbjct: 515 SVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAASDQTQVLE 574

Query: 241 LVELHLPTE-HIVHTEL 256
           L  + LPTE HIVHTEL
Sbjct: 575 LETVQLPTEQHIVHTEL 591

[Close]
BLAST of MC09g_new0479 vs. ExPASy TrEMBL
Match: A0A5A7SWQ6 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003060 PE=3 SV=1)

HSP 1 Score: 476 bits (1224), Expect = 6.81e-164
Identity = 239/257 (93.00%), Postives = 248/257 (96.50%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           MHFTIARSE+VR+TEVRVSAPGDSPNTDGIHITQSTNVV+QN KISTGDDCISIVNASSG
Sbjct: 336 MHFTIARSETVRITEVRVSAPGDSPNTDGIHITQSTNVVVQNSKISTGDDCISIVNASSG 395

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV
Sbjct: 396 IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 455

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
           RFENVRMEDVENPIIIDQFYCDSPT+CETQTSAVKISQIMYRNISGTTTSKNAMKFACSD
Sbjct: 456 RFENVRMEDVENPIIIDQFYCDSPTTCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 515

Query: 181 SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPE 240
           SVPCSNI+LSNVNLEKTDGTVETYCH+AQGFGYG+VHPSADCLTSSD   AASDQT+V E
Sbjct: 516 SVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGIVHPSADCLTSSDNNFAASDQTQVLE 575

Query: 241 LVELHLPTE-HIVHTEL 256
           L  + LPTE HIVHTEL
Sbjct: 576 LETVQLPTEQHIVHTEL 592

[Close]
BLAST of MC09g_new0479 vs. ExPASy TrEMBL
Match: A0A0A0KQ65 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G600960 PE=3 SV=1)

HSP 1 Score: 469 bits (1207), Expect = 4.41e-163
Identity = 235/257 (91.44%), Postives = 247/257 (96.11%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           MHFTIARSE+VR+T VRVSAPGDSPNTDGIHITQSTNVV+QNCKISTGDDCISIVNASSG
Sbjct: 213 MHFTIARSETVRITGVRVSAPGDSPNTDGIHITQSTNVVVQNCKISTGDDCISIVNASSG 272

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTA+LRETTNGVRIKTWQGGSGYVR+V
Sbjct: 273 IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAYLRETTNGVRIKTWQGGSGYVRAV 332

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
           RFENVRMEDVENPIIIDQFYCDSPT+CE+QTSAVKISQIMYRNISGTTTSKNAMKFACSD
Sbjct: 333 RFENVRMEDVENPIIIDQFYCDSPTTCESQTSAVKISQIMYRNISGTTTSKNAMKFACSD 392

Query: 181 SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPE 240
           SVPCSNI+LSNVNLEKTDGTVETYCH+AQGFGYGVVHPSADCLTSSD   A+SDQT+V E
Sbjct: 393 SVPCSNIILSNVNLEKTDGTVETYCHAAQGFGYGVVHPSADCLTSSDNNFASSDQTQVLE 452

Query: 241 LVELHLPTE-HIVHTEL 256
           L  + L TE HIVHTEL
Sbjct: 453 LETVQLSTEQHIVHTEL 469

[Close]
BLAST of MC09g_new0479 vs. TAIR 10
Match: AT1G23460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 370.9 bits (951), Expect = 8.1e-103
Identity = 183/243 (75.31%), Postives = 210/243 (86.42%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           M+F IARS+SVRV++V VS+PGDSPNTDGIHIT STNV++Q+CKI TGDDC+SIVNASS 
Sbjct: 214 MNFIIARSDSVRVSKVMVSSPGDSPNTDGIHITGSTNVILQDCKIGTGDDCVSIVNASSN 273

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           IKMK I CGPGHGISIGSLGKDN+TGIVT+VVLDTA LRETTNG+RIKT+QGGSGYV+ +
Sbjct: 274 IKMKNIYCGPGHGISIGSLGKDNTTGIVTQVVLDTALLRETTNGLRIKTYQGGSGYVQGI 333

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
           RF NV M+DV NPI+IDQFYCDSPT+C+ QTSAVKISQIMYRNI+GTT S  A+KFACSD
Sbjct: 334 RFTNVEMQDVANPILIDQFYCDSPTTCQNQTSAVKISQIMYRNITGTTKSAKAIKFACSD 393

Query: 181 SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPE 240
           +VPCS+IVL+NVNLE  DG VE YC+SA+GFGYGV+HPSADCL S D      DQT   E
Sbjct: 394 TVPCSHIVLNNVNLEGNDGQVEAYCNSAEGFGYGVIHPSADCLYSHD--DKGLDQTHKSE 453

Query: 241 LVE 244
             E
Sbjct: 454 EAE 454

[Close]
BLAST of MC09g_new0479 vs. TAIR 10
Match: AT1G70500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 344.4 bits (882), Expect = 8.1e-95
Identity = 171/256 (66.80%), Postives = 205/256 (80.08%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           MH  I RS +VR++ V V++PGDSPNTDGIHIT ST+VV+Q+ KISTGDDC+SIVN S+ 
Sbjct: 214 MHLIIQRSTTVRISRVMVTSPGDSPNTDGIHITASTDVVVQDSKISTGDDCVSIVNGSAK 273

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           IKMK I CGPGHGISIGSLG+ +S G VT VVL+TAFL+ TTNG+RIKTWQGG+GYV+ V
Sbjct: 274 IKMKRIYCGPGHGISIGSLGQGHSKGTVTAVVLETAFLKNTTNGLRIKTWQGGNGYVKGV 333

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
           RFENV M+DV NPIIIDQFYCDSP++C+ QTSAV IS+IMYRNI+GTT S  A+ F CSD
Sbjct: 334 RFENVVMQDVANPIIIDQFYCDSPSTCQNQTSAVHISEIMYRNITGTTKSSKAINFKCSD 393

Query: 181 SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEVPE 240
           +VPCS+IVL+N+NLE  DG VE YC+SA+GFGYGVVHPSADCL S D  S       + E
Sbjct: 394 AVPCSHIVLNNINLEGNDGKVEAYCNSAEGFGYGVVHPSADCLYSHDDKSLNQTDQYLAE 453

Query: 241 LVELHLPTEHIVHTEL 257
            + +   TE+  H EL
Sbjct: 454 TIMVEKETEN-AHDEL 468

[Close]
BLAST of MC09g_new0479 vs. TAIR 10
Match: AT1G80170.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 2.0e-61
Identity = 118/240 (49.17%), Postives = 156/240 (65.00%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           MH  +     V ++ ++V AP  SPNTDGIHI+ S  +VI N  +STGDDCISIV  S+ 
Sbjct: 201 MHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQ 260

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           I +  I CGPGHGISIGSLGK  S   V  + +DTA + +T NGVRIKTWQGGSG V  +
Sbjct: 261 ISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKI 320

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
            F N++M +V NPIIIDQ+YCDS   C  QTSA+ I  I + ++ GT+ SK A+K +CSD
Sbjct: 321 IFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHVRGTSASKEAIKISCSD 380

Query: 181 SVPCSNIVLSNVNLEKT--DGTVETYCHSAQGFGYGVVHPSADCLTSSDKYSAASDQTEV 239
           S PC NI+L +++LE +  DG  E++C  A G   G V+P   CL+    +   S Q+ +
Sbjct: 381 SSPCRNILLQDIDLEPSNGDGFTESFCWEAYGSSSGQVYP-PPCLSDDTSFLEQSVQSGI 439

[Close]
BLAST of MC09g_new0479 vs. TAIR 10
Match: AT3G07970.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 211.5 bits (537), Expect = 8.2e-55
Identity = 99/194 (51.03%), Postives = 141/194 (72.68%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           MH T    ++V+   + V++P DSPNTDGIH++ + N++IQ+  + TGDDCISIV+ S  
Sbjct: 217 MHLTFQDCKNVKALNLMVTSPADSPNTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSEN 276

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           ++  GI+CGPGHGISIGSLG+DNS   V+ VV++ A L  TTNGVRIKTWQGG G  +++
Sbjct: 277 VRATGITCGPGHGISIGSLGEDNSEAYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNI 336

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
            F+++ M++V NPIII+Q YCD   +C  Q SAV++S ++Y+NI GT++   A+KF CS 
Sbjct: 337 IFQDIIMKNVTNPIIINQDYCDRVEACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSK 396

Query: 181 SVPCSNIVLSNVNL 195
           ++PC  I + NV L
Sbjct: 397 NIPCRGISMQNVKL 410

[Close]
BLAST of MC09g_new0479 vs. TAIR 10
Match: AT3G57510.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 210.3 bits (534), Expect = 1.8e-54
Identity = 106/218 (48.62%), Postives = 147/218 (67.43%), Query Frame = 0 

Query: 1   MHFTIARSESVRVTEVRVSAPGDSPNTDGIHITQSTNVVIQNCKISTGDDCISIVNASSG 60
           +  +I +   V V+ V ++APGDSPNTDGIHIT + N+ + N  I TGDDCISI + +  
Sbjct: 216 IQISIEKCNKVEVSNVEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQN 275

Query: 61  IKMKGISCGPGHGISIGSLGKDNSTGIVTKVVLDTAFLRETTNGVRIKTWQGGSGYVRSV 120
           +++  ++CGPGHGISIGSLG DNS   V+ + +D A   E+ NGVRIKT+QGGSG  +++
Sbjct: 276 LQIFDLTCGPGHGISIGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQGGSGTAKNI 335

Query: 121 RFENVRMEDVENPIIIDQFYCDSPTSCETQTSAVKISQIMYRNISGTTTSKNAMKFACSD 180
           +F+N+RME+V+NPIIIDQ YCD    CE Q SAV++  ++Y+NISGT+ +  A+   CS+
Sbjct: 336 KFQNIRMENVKNPIIIDQDYCDK-DKCEDQESAVQVKNVVYKNISGTSATDVAITLNCSE 395

Query: 181 SVPCSNIVLSNVNLEKTDGTVETYCHSAQGFGYGVVHP 219
             PC  IVL NV ++   GT    C +A     G V P
Sbjct: 396 KYPCQGIVLENVKIK--GGTAS--CKNANVKNQGTVSP 428

The following BLAST results are available for this feature:
BLAST of MC09g_new0479 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q94AJ52.8e-6049.17Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P353363.6e-5550.23Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Q9FY191.0e-5448.88Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1[more]
Q9SFB71.2e-5351.03Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2[more]
O231472.6e-5348.62Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1[more]
BLAST of MC09g_new0479 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022151049.13.38e-179100.00probable polygalacturonase At1g80170 [Momordica charantia][more]
XP_008446196.16.78e-16492.61PREDICTED: probable polygalacturonase At1g80170 [Cucumis melo][more]
TYK15614.11.36e-16393.00polygalacturonase [Cucumis melo var. makuwa][more]
KAA0034306.11.41e-16393.00polygalacturonase [Cucumis melo var. makuwa][more]
XP_011655619.19.10e-16391.44probable polygalacturonase At1g80170 isoform X1 [Cucumis sativus][more]
BLAST of MC09g_new0479 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1DBU91.64e-179100.00probable polygalacturonase At1g80170 OS=Momordica charantia OX=3673 GN=LOC111019... [more]
A0A1S3BF583.28e-16492.61probable polygalacturonase At1g80170 OS=Cucumis melo OX=3656 GN=LOC103489000 PE=... [more]
A0A5D3CXE46.60e-16493.00Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001... [more]
A0A5A7SWQ66.81e-16493.00Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003... [more]
A0A0A0KQ654.41e-16391.44Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G600960 PE=3 SV=1[more]
BLAST of MC09g_new0479 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G23460.18.1e-10375.31Pectin lyase-like superfamily protein [more]
AT1G70500.18.1e-9566.80Pectin lyase-like superfamily protein [more]
AT1G80170.12.0e-6149.17Pectin lyase-like superfamily protein [more]
AT3G07970.18.2e-5551.03Pectin lyase-like superfamily protein [more]
AT3G57510.11.8e-5448.62Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 8..34
e-value: 180.0
score: 11.0
coord: 117..138
e-value: 4300.0
score: 2.7
coord: 58..78
e-value: 8900.0
score: 0.5
coord: 184..205
e-value: 6800.0
score: 1.3
coord: 35..56
e-value: 11.0
score: 15.0
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 1..205
e-value: 3.3E-64
score: 217.0
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 65..78
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 1..254
e-value: 4.5E-75
score: 255.1
NoneNo IPR availablePANTHERPTHR31375:SF205BNAA08G20470D PROTEINcoord: 1..235
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 1..235
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 1..224

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g_new0479.1MC09g_new0479.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008234 cysteine-type peptidase activity
molecular_function GO:0004650 polygalacturonase activity