MC09g_new0448 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g_new0448
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionProtein of unknown function (DUF1262)
LocationMC09: 21479568 .. 21481415 (-)
RNA-Seq ExpressionMC09g_new0448
SyntenyMC09g_new0448
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTCCTTCATTTGCAGCATCTCAAATTCCTTTCTCAAGACTTGAAGCTGTGCATGCTTTGTTCTCGAAGAGCCTTGATACTTTTTCTTCATTGATTCCCAAATATCCTTGGATGATTCTTTGCAAGGAATAGTCTCCAGGATAGAACGATCAATGGCCTGAAACAGATAATTTTTTGCCTTCAAGTCTTTCAACTTGAGTCCCTCCAACTCCTTTTGTTGAGCGTCTGTGAGAATCTCTCCAGCTGCAGGTGTTGCAACACCCGTTTCAACAACATGCCAGTATTCTTTGGACCGCAAGAAATTTTCCATCAACATGCTCCAATGATCGTAGTGACCATCAAAACGAGGAATAGCTGCTTGCACAAAGTTACCCTCCGAGGCCATTTATGCTGCAAAATAGAAAAACAATATGAGCAAAACAGAACTTAATGCTACTGCACTGATTGCTCTCACTTCCTTGTGTTTTCCTTTCCCTGTGTTTCCCTTTTAGAATTTTCACACTCTTCCCAAATATCAAAATAAACACAGGGGACAACAAATTTGAGGAAGGAATGATTTTCACTCAAATCAATTGAGAATACAGCACCTATTTATTTAGGCAAATACATAACCAAATTAGACAAAAATAGCACTCCACGACCAACTAAGTCATCAACTAGTTTCCTAAAGACTAGGATAATATTGACTTAACATATGACATTAATTAGTAAATAACTAAAAATAGTTTTTATGCTAACATATTTATGAGTTGCAGGTTAGCATATACAAGTTCAAAGGAAGATGAGATGAGCCGCTGCTGCTGGTGCTGCTTCGTCATCAACGATAAACGACCACAACTCTTCGACCCAAAAAATGCTTATCAACAGTTCGAGATTAGTAGTAGAATAATAGAGTGTGGAGGACAACCCTGGGGTTTTGTGTCAAAGTCCGTAGCTCCTGACGGGATTCCTCCAAATTTCCTTAGACACGAGGGTTGGAAGGCTGGAACCAAACCCCTTAACAAAAACTTGGAGCTCACGGAAGCGCTCGGCCTCGACGCCGCGCTGCGTGGCCGCCTCCCTGATCTCAGCTTCCCGCTGCCAGCCAAATGTTCGGACCCGGTGGTGGTGGGGAAGTGGTACTGCCCTTTCATTTTCGTTCGAGATGGGGAGGTTGGTTCTCAAGTGAGTAACTCGCCGTACTACGAAATGACCCTTCAGCAGAATTGGGAGGAGATATTTGGGTGTGGGAATTTGGGGGGAGGTGAGCGAGGGGTGGATTTTGATGTTTCTGTAGAAAGGGAAGTGATTTCGATTGCGGGTCAGCGGGCCGATGGTAGAGAAGTTGGCGATGGGGTTGTATGGTTTGGTTCGAGGGGGGTTGGGCTGAGCTTGGCGATTGAGGAGAGAGTGAAATGGGAGGAGGAGAGGGCGGGATTTGAGTTTGGAAAAGAAAAAGAAGAGAAATATGTGAAGATGAATAGAAGAGAGGATTTTGGAGGGGTGGAAGAATGGAGGAGATTTGGATGTTATGTGTTGGTCGAGAGATTTGTGCTTAAGAGAATGGATGGAAGTTTGGTTCTTACCTGGGAATTCAGGCACACCCACCAAATTAGAACCAAATGGAACTGAGATGATATTAGCGTAGCATATTAATTATCACGTGTATATTGTTCATCTTGTTACTTTGGTTGGGTAGAGAATTCATAGTAATTTGTATTTGGAGTTACCGAAATTTGATTATATTTGTTTTGCTTTTCTATATGAATCTTTATTTGTATTATATTTTTAGACAAATTTTTTTGACACGTAGAAGTAAATGTACCTAACTATCCCTCGTTTTGTATTCATTAGAGCGTTAAAGGGAG

mRNA sequence

ATGAGTTGCAGGTTAGCATATACAAGTTCAAAGGAAGATGAGATGAGCCGCTGCTGCTGGTGCTGCTTCGTCATCAACGATAAACGACCACAACTCTTCGACCCAAAAAATGCTTATCAACAGTTCGAGATTAGTAGTAGAATAATAGAGTGTGGAGGACAACCCTGGGGTTTTGTGTCAAAGTCCGTAGCTCCTGACGGGATTCCTCCAAATTTCCTTAGACACGAGGGTTGGAAGGCTGGAACCAAACCCCTTAACAAAAACTTGGAGCTCACGGAAGCGCTCGGCCTCGACGCCGCGCTGCGTGGCCGCCTCCCTGATCTCAGCTTCCCGCTGCCAGCCAAATGTTCGGACCCGGTGGTGGTGGGGAAGTGGTACTGCCCTTTCATTTTCGTTCGAGATGGGGAGGTTGGTTCTCAAGTGAGTAACTCGCCGTACTACGAAATGACCCTTCAGCAGAATTGGGAGGAGATATTTGGGTGTGGGAATTTGGGGGGAGGTGAGCGAGGGGTGGATTTTGATGTTTCTGTAGAAAGGGAAGTGATTTCGATTGCGGGTCAGCGGGCCGATGGTAGAGAAGTTGGCGATGGGGTTGTATGGTTTGGTTCGAGGGGGGTTGGGCTGAGCTTGGCGATTGAGGAGAGAGTGAAATGGGAGGAGGAGAGGGCGGGATTTGAGTTTGGAAAAGAAAAAGAAGAGAAATATGTGAAGATGAATAGAAGAGAGGATTTTGGAGGGGTGGAAGAATGGAGGAGATTTGGATGTTATGTGTTGGTCGAGAGATTTGTGCTTAAGAGAATGGATGGAAGTTTGGTTCTTACCTGGGAATTCAGGCACACCCACCAAATTAGAACCAAATGGAACTGA

Coding sequence (CDS)

ATGAGTTGCAGGTTAGCATATACAAGTTCAAAGGAAGATGAGATGAGCCGCTGCTGCTGGTGCTGCTTCGTCATCAACGATAAACGACCACAACTCTTCGACCCAAAAAATGCTTATCAACAGTTCGAGATTAGTAGTAGAATAATAGAGTGTGGAGGACAACCCTGGGGTTTTGTGTCAAAGTCCGTAGCTCCTGACGGGATTCCTCCAAATTTCCTTAGACACGAGGGTTGGAAGGCTGGAACCAAACCCCTTAACAAAAACTTGGAGCTCACGGAAGCGCTCGGCCTCGACGCCGCGCTGCGTGGCCGCCTCCCTGATCTCAGCTTCCCGCTGCCAGCCAAATGTTCGGACCCGGTGGTGGTGGGGAAGTGGTACTGCCCTTTCATTTTCGTTCGAGATGGGGAGGTTGGTTCTCAAGTGAGTAACTCGCCGTACTACGAAATGACCCTTCAGCAGAATTGGGAGGAGATATTTGGGTGTGGGAATTTGGGGGGAGGTGAGCGAGGGGTGGATTTTGATGTTTCTGTAGAAAGGGAAGTGATTTCGATTGCGGGTCAGCGGGCCGATGGTAGAGAAGTTGGCGATGGGGTTGTATGGTTTGGTTCGAGGGGGGTTGGGCTGAGCTTGGCGATTGAGGAGAGAGTGAAATGGGAGGAGGAGAGGGCGGGATTTGAGTTTGGAAAAGAAAAAGAAGAGAAATATGTGAAGATGAATAGAAGAGAGGATTTTGGAGGGGTGGAAGAATGGAGGAGATTTGGATGTTATGTGTTGGTCGAGAGATTTGTGCTTAAGAGAATGGATGGAAGTTTGGTTCTTACCTGGGAATTCAGGCACACCCACCAAATTAGAACCAAATGGAACTGA

Protein sequence

MSCRLAYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVSKSVAPDGIPPNFLRHEGWKAGTKPLNKNLELTEALGLDAALRGRLPDLSFPLPAKCSDPVVVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERGVDFDVSVEREVISIAGQRADGREVGDGVVWFGSRGVGLSLAIEERVKWEEERAGFEFGKEKEEKYVKMNRREDFGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKWN
Homology
BLAST of MC09g_new0448 vs. NCBI nr
Match: XP_022149022.1 (uncharacterized protein LOC111017542 [Momordica charantia])

HSP 1 Score: 606 bits (1562), Expect = 4.39e-219
Identity = 287/288 (99.65%), Postives = 288/288 (100.00%), Query Frame = 0

Query: 1   MSCRLAYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVS 60
           MSCRLAYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVS
Sbjct: 1   MSCRLAYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVS 60

Query: 61  KSVAPDGIPPNFLRHEGWKAGTKPLNKNLELTEALGLDAALRGRLPDLSFPLPAKCSDPV 120
           KSVAPDGIPPNFLRHEGWKAGTKPLNKNLELTEALGLDAALRGRLPDLSFPLPAKCSDPV
Sbjct: 61  KSVAPDGIPPNFLRHEGWKAGTKPLNKNLELTEALGLDAALRGRLPDLSFPLPAKCSDPV 120

Query: 121 VVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERGVDFDVSVERE 180
           VVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERGVDFDVSVERE
Sbjct: 121 VVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERGVDFDVSVERE 180

Query: 181 VISIAGQRADGREVGDGVVWFGSRGVGLSLAIEERVKWEEERAGFEFGKEKEEKYVKMNR 240
           VISIAGQRADGREVGDGVVWFGSRGVGLSLAIEERVKWE+ERAGFEFGKEKEEKYVKMNR
Sbjct: 181 VISIAGQRADGREVGDGVVWFGSRGVGLSLAIEERVKWEDERAGFEFGKEKEEKYVKMNR 240

Query: 241 REDFGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKWN 288
           REDFGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKWN
Sbjct: 241 REDFGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKWN 288

BLAST of MC09g_new0448 vs. NCBI nr
Match: XP_022149086.1 (uncharacterized protein LOC111017584 [Momordica charantia])

HSP 1 Score: 465 bits (1197), Expect = 7.59e-162
Identity = 228/284 (80.28%), Postives = 250/284 (88.03%), Query Frame = 0

Query: 5   LAYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVSKSVA 64
           LA TSSKED+ + CC  CF I D  PQLFDP NAYQQF+IS+  ++C G P GF++ S+A
Sbjct: 112 LACTSSKEDDETSCC--CFGIPDTPPQLFDPTNAYQQFQISN-YVQCAG-PCGFMANSIA 171

Query: 65  PDGIPPNFLRHEGWKAGTKPL-NKNLELTEALGLDAALRGRLPDLSFPLPAKCSDPVVVG 124
           PDG+PP FLR  GW+A TK   NKNLELT+ALGLDAALR  LP L+F LPAK SDPVVVG
Sbjct: 172 PDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRAHLPALNFSLPAKSSDPVVVG 231

Query: 125 KWYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERGVDFDVSVEREVIS 184
           KWYCPFIFVRDGEVGSQVSNSPYYEMTLQQ+WEEIFGCGNLGGGERGVDFDVSVE+EVI 
Sbjct: 232 KWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGGERGVDFDVSVEKEVIL 291

Query: 185 IAGQRADGREVGDGVVWFGSRGVGLSLAIEERVKWEEERAGFEFGKEKEEKYVKMNRRED 244
           IAGQR DGREVGDGVVWFGS GVGLSLAI ERVKWEEERAGFEFGKEKE+KYVK+ RRE+
Sbjct: 292 IAGQRTDGREVGDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGKEKEKKYVKVKRREE 351

Query: 245 FGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKW 287
           +GGV EW+RFGCYVL+ERFVLKRMDGSLVLTWEF+HTHQIRTKW
Sbjct: 352 YGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKW 391

BLAST of MC09g_new0448 vs. NCBI nr
Match: XP_022149112.1 (uncharacterized protein LOC111017603 [Momordica charantia])

HSP 1 Score: 419 bits (1076), Expect = 1.63e-143
Identity = 216/284 (76.06%), Postives = 234/284 (82.39%), Query Frame = 0

Query: 5   LAYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVSKSVA 64
           LA TSSKED  SR     + I+D   QL DPKN YQQF+IS+ I  CG +P GF+SKSVA
Sbjct: 111 LACTSSKEDNTSRRSRFFYRIHDAPAQLLDPKNTYQQFQISNYIY-CG-KPNGFISKSVA 170

Query: 65  PDGIPPNFLRHEGWKAGTKPLNKNLEL-TEALGLDAALRGRLPDLSFPLPAKCSDPVVVG 124
           PDG+PP  LR +GW+A  +PLN N  L TEALGLDAALR RLPDL+FP      +PVVVG
Sbjct: 171 PDGVPPELLRRKGWRAYPEPLNNNNSLPTEALGLDAALRARLPDLAFP-----PNPVVVG 230

Query: 125 KWYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERGVDFDVSVEREVIS 184
           KWYCPFIFVRDG V SQ+SNSPYYEMTLQQNWEEIFGCG+LGGG R VDFDVSVEREVIS
Sbjct: 231 KWYCPFIFVRDGAVESQMSNSPYYEMTLQQNWEEIFGCGSLGGGGRVVDFDVSVEREVIS 290

Query: 185 IAGQRADGREVGDGVVWFGSRGVGLSLAIEERVKWEEERAGFEFGKEKEEKYVKMNRRED 244
           IAGQ A GR  GDGV+WFGS GVGLSLAI ERVKWEEERAGFEFGKEKE+KYVK+ RRE+
Sbjct: 291 IAGQAAGGRNAGDGVMWFGSSGVGLSLAIVERVKWEEERAGFEFGKEKEKKYVKVKRREE 350

Query: 245 FGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKW 287
           F    EWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKW
Sbjct: 351 FKERGEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKW 387

BLAST of MC09g_new0448 vs. NCBI nr
Match: KAG6574010.1 (hypothetical protein SDJN03_27897, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 352 bits (904), Expect = 1.75e-117
Identity = 183/284 (64.44%), Postives = 217/284 (76.41%), Query Frame = 0

Query: 5   LAYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVSKSVA 64
           LA TSSKE+E S    CC+ + D  PQLFDPKNAYQQF+IS  I  CGG P G+++KS+A
Sbjct: 110 LACTSSKEEETSG--RCCYSVPDPPPQLFDPKNAYQQFQISDYIY-CGG-PNGYITKSMA 169

Query: 65  PDGIPPNFLRHEGWKAGTKPLNKNLELTEALGLDAALRGRLPDLSFPLPAKCSDPVVVGK 124
           PDG+PP  L  +G +A T+PL KN E TEALGL+ +LRGRLP++      K SDPVVVGK
Sbjct: 170 PDGVPPKRLSRKGLRAYTQPL-KNFEPTEALGLNPSLRGRLPEI------KSSDPVVVGK 229

Query: 125 WYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERGVDFDVSVEREVISI 184
           WYCPFIF+R+G+V SQ+SNSPYYEMTL++NW EIFGC + G G  GVD DV VEREV S+
Sbjct: 230 WYCPFIFIREGDVSSQMSNSPYYEMTLRRNWFEIFGCESSGEGNNGVDVDVYVEREVASV 289

Query: 185 AGQRADGREVG-DGVVWFGSRGVGLSLAIEERVKWEEERAGFEFGKEKEEKYVKMNRRED 244
           AG  A  R  G DG VWF + GVGLS AI ERVKWEE R GF + +E EEK +++ RRE+
Sbjct: 290 AGGAAARRRYGGDGFVWFEAAGVGLSTAIVERVKWEEGREGFGWVEEGEEKKIRVRRREE 349

Query: 245 FGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKW 287
             GV  WRRFGCYVL+ERFVLKRMDGS+VLTWEFRHTHQ+ TKW
Sbjct: 350 LKGVGIWRRFGCYVLIERFVLKRMDGSVVLTWEFRHTHQLTTKW 382

BLAST of MC09g_new0448 vs. NCBI nr
Match: XP_022945417.1 (uncharacterized protein LOC111449654 [Cucurbita moschata])

HSP 1 Score: 350 bits (899), Expect = 1.00e-116
Identity = 183/284 (64.44%), Postives = 217/284 (76.41%), Query Frame = 0

Query: 5   LAYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVSKSVA 64
           LA TSSKE+E S    CC+ + D  PQLFDPKNAYQQF+IS  I  CGG P G+++KS+A
Sbjct: 110 LACTSSKEEETSG--RCCYSVLDPPPQLFDPKNAYQQFQISDYIY-CGG-PNGYITKSMA 169

Query: 65  PDGIPPNFLRHEGWKAGTKPLNKNLELTEALGLDAALRGRLPDLSFPLPAKCSDPVVVGK 124
           PDG+PP  L  +G +A T+PL KN E TEALGL+ +LRGRLP++      K SDPVVVGK
Sbjct: 170 PDGVPPKRLSRKGLRAYTQPL-KNFEPTEALGLNPSLRGRLPEI------KSSDPVVVGK 229

Query: 125 WYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERGVDFDVSVEREVISI 184
           WYCPFIF+R+G+V SQ+SNSPYYEMTL+++W EIFGC + G G  GVD DV VEREV S+
Sbjct: 230 WYCPFIFIREGDVSSQMSNSPYYEMTLRRDWFEIFGCESSGEGNNGVDVDVYVEREVASV 289

Query: 185 AGQRADGREVG-DGVVWFGSRGVGLSLAIEERVKWEEERAGFEFGKEKEEKYVKMNRRED 244
           AG  A  R  G DG VWF + GVGLS AI ERVKWEE R GF + +E EEK +++ RRE+
Sbjct: 290 AGGAAARRRYGGDGFVWFEAAGVGLSTAIVERVKWEEGREGFGWVEEGEEKKIRVRRREE 349

Query: 245 FGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKW 287
             GV  WRRFGCYVLVERFVLKRMDGS+VLTWEFRHTHQ+ TKW
Sbjct: 350 LKGVGIWRRFGCYVLVERFVLKRMDGSVVLTWEFRHTHQLTTKW 382

BLAST of MC09g_new0448 vs. ExPASy TrEMBL
Match: A0A6J1D6P2 (uncharacterized protein LOC111017542 OS=Momordica charantia OX=3673 GN=LOC111017542 PE=4 SV=1)

HSP 1 Score: 606 bits (1562), Expect = 2.12e-219
Identity = 287/288 (99.65%), Postives = 288/288 (100.00%), Query Frame = 0

Query: 1   MSCRLAYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVS 60
           MSCRLAYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVS
Sbjct: 1   MSCRLAYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVS 60

Query: 61  KSVAPDGIPPNFLRHEGWKAGTKPLNKNLELTEALGLDAALRGRLPDLSFPLPAKCSDPV 120
           KSVAPDGIPPNFLRHEGWKAGTKPLNKNLELTEALGLDAALRGRLPDLSFPLPAKCSDPV
Sbjct: 61  KSVAPDGIPPNFLRHEGWKAGTKPLNKNLELTEALGLDAALRGRLPDLSFPLPAKCSDPV 120

Query: 121 VVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERGVDFDVSVERE 180
           VVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERGVDFDVSVERE
Sbjct: 121 VVGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERGVDFDVSVERE 180

Query: 181 VISIAGQRADGREVGDGVVWFGSRGVGLSLAIEERVKWEEERAGFEFGKEKEEKYVKMNR 240
           VISIAGQRADGREVGDGVVWFGSRGVGLSLAIEERVKWE+ERAGFEFGKEKEEKYVKMNR
Sbjct: 181 VISIAGQRADGREVGDGVVWFGSRGVGLSLAIEERVKWEDERAGFEFGKEKEEKYVKMNR 240

Query: 241 REDFGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKWN 288
           REDFGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKWN
Sbjct: 241 REDFGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKWN 288

BLAST of MC09g_new0448 vs. ExPASy TrEMBL
Match: A0A6J1D7B1 (uncharacterized protein LOC111017584 OS=Momordica charantia OX=3673 GN=LOC111017584 PE=4 SV=1)

HSP 1 Score: 465 bits (1197), Expect = 3.67e-162
Identity = 228/284 (80.28%), Postives = 250/284 (88.03%), Query Frame = 0

Query: 5   LAYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVSKSVA 64
           LA TSSKED+ + CC  CF I D  PQLFDP NAYQQF+IS+  ++C G P GF++ S+A
Sbjct: 112 LACTSSKEDDETSCC--CFGIPDTPPQLFDPTNAYQQFQISN-YVQCAG-PCGFMANSIA 171

Query: 65  PDGIPPNFLRHEGWKAGTKPL-NKNLELTEALGLDAALRGRLPDLSFPLPAKCSDPVVVG 124
           PDG+PP FLR  GW+A TK   NKNLELT+ALGLDAALR  LP L+F LPAK SDPVVVG
Sbjct: 172 PDGVPPRFLRRRGWRAYTKTFKNKNLELTQALGLDAALRAHLPALNFSLPAKSSDPVVVG 231

Query: 125 KWYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERGVDFDVSVEREVIS 184
           KWYCPFIFVRDGEVGSQVSNSPYYEMTLQQ+WEEIFGCGNLGGGERGVDFDVSVE+EVI 
Sbjct: 232 KWYCPFIFVRDGEVGSQVSNSPYYEMTLQQSWEEIFGCGNLGGGERGVDFDVSVEKEVIL 291

Query: 185 IAGQRADGREVGDGVVWFGSRGVGLSLAIEERVKWEEERAGFEFGKEKEEKYVKMNRRED 244
           IAGQR DGREVGDGVVWFGS GVGLSLAI ERVKWEEERAGFEFGKEKE+KYVK+ RRE+
Sbjct: 292 IAGQRTDGREVGDGVVWFGSWGVGLSLAIVERVKWEEERAGFEFGKEKEKKYVKVKRREE 351

Query: 245 FGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKW 287
           +GGV EW+RFGCYVL+ERFVLKRMDGSLVLTWEF+HTHQIRTKW
Sbjct: 352 YGGVGEWKRFGCYVLIERFVLKRMDGSLVLTWEFKHTHQIRTKW 391

BLAST of MC09g_new0448 vs. ExPASy TrEMBL
Match: A0A6J1D6Y4 (uncharacterized protein LOC111017603 OS=Momordica charantia OX=3673 GN=LOC111017603 PE=4 SV=1)

HSP 1 Score: 419 bits (1076), Expect = 7.89e-144
Identity = 216/284 (76.06%), Postives = 234/284 (82.39%), Query Frame = 0

Query: 5   LAYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVSKSVA 64
           LA TSSKED  SR     + I+D   QL DPKN YQQF+IS+ I  CG +P GF+SKSVA
Sbjct: 111 LACTSSKEDNTSRRSRFFYRIHDAPAQLLDPKNTYQQFQISNYIY-CG-KPNGFISKSVA 170

Query: 65  PDGIPPNFLRHEGWKAGTKPLNKNLEL-TEALGLDAALRGRLPDLSFPLPAKCSDPVVVG 124
           PDG+PP  LR +GW+A  +PLN N  L TEALGLDAALR RLPDL+FP      +PVVVG
Sbjct: 171 PDGVPPELLRRKGWRAYPEPLNNNNSLPTEALGLDAALRARLPDLAFP-----PNPVVVG 230

Query: 125 KWYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERGVDFDVSVEREVIS 184
           KWYCPFIFVRDG V SQ+SNSPYYEMTLQQNWEEIFGCG+LGGG R VDFDVSVEREVIS
Sbjct: 231 KWYCPFIFVRDGAVESQMSNSPYYEMTLQQNWEEIFGCGSLGGGGRVVDFDVSVEREVIS 290

Query: 185 IAGQRADGREVGDGVVWFGSRGVGLSLAIEERVKWEEERAGFEFGKEKEEKYVKMNRRED 244
           IAGQ A GR  GDGV+WFGS GVGLSLAI ERVKWEEERAGFEFGKEKE+KYVK+ RRE+
Sbjct: 291 IAGQAAGGRNAGDGVMWFGSSGVGLSLAIVERVKWEEERAGFEFGKEKEKKYVKVKRREE 350

Query: 245 FGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKW 287
           F    EWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKW
Sbjct: 351 FKERGEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKW 387

BLAST of MC09g_new0448 vs. ExPASy TrEMBL
Match: A0A6J1G0V4 (uncharacterized protein LOC111449654 OS=Cucurbita moschata OX=3662 GN=LOC111449654 PE=4 SV=1)

HSP 1 Score: 350 bits (899), Expect = 4.86e-117
Identity = 183/284 (64.44%), Postives = 217/284 (76.41%), Query Frame = 0

Query: 5   LAYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVSKSVA 64
           LA TSSKE+E S    CC+ + D  PQLFDPKNAYQQF+IS  I  CGG P G+++KS+A
Sbjct: 110 LACTSSKEEETSG--RCCYSVLDPPPQLFDPKNAYQQFQISDYIY-CGG-PNGYITKSMA 169

Query: 65  PDGIPPNFLRHEGWKAGTKPLNKNLELTEALGLDAALRGRLPDLSFPLPAKCSDPVVVGK 124
           PDG+PP  L  +G +A T+PL KN E TEALGL+ +LRGRLP++      K SDPVVVGK
Sbjct: 170 PDGVPPKRLSRKGLRAYTQPL-KNFEPTEALGLNPSLRGRLPEI------KSSDPVVVGK 229

Query: 125 WYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERGVDFDVSVEREVISI 184
           WYCPFIF+R+G+V SQ+SNSPYYEMTL+++W EIFGC + G G  GVD DV VEREV S+
Sbjct: 230 WYCPFIFIREGDVSSQMSNSPYYEMTLRRDWFEIFGCESSGEGNNGVDVDVYVEREVASV 289

Query: 185 AGQRADGREVG-DGVVWFGSRGVGLSLAIEERVKWEEERAGFEFGKEKEEKYVKMNRRED 244
           AG  A  R  G DG VWF + GVGLS AI ERVKWEE R GF + +E EEK +++ RRE+
Sbjct: 290 AGGAAARRRYGGDGFVWFEAAGVGLSTAIVERVKWEEGREGFGWVEEGEEKKIRVRRREE 349

Query: 245 FGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKW 287
             GV  WRRFGCYVLVERFVLKRMDGS+VLTWEFRHTHQ+ TKW
Sbjct: 350 LKGVGIWRRFGCYVLVERFVLKRMDGSVVLTWEFRHTHQLTTKW 382

BLAST of MC09g_new0448 vs. ExPASy TrEMBL
Match: A0A5A7SZ71 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00480 PE=4 SV=1)

HSP 1 Score: 340 bits (873), Expect = 2.68e-113
Identity = 174/285 (61.05%), Postives = 205/285 (71.93%), Query Frame = 0

Query: 5   LAYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVSKSVA 64
           LAY  SKE+  SR       + D  PQ FDP N YQ+FEIS+ +    G+P GF  KSVA
Sbjct: 90  LAYMCSKEENGSRL----LGVADTPPQKFDPTNIYQEFEISNDMYY--GKPNGFNFKSVA 149

Query: 65  PDGIPPNFLRHEGWKAGTKPLNKNLELTEALGLDAALRGRLPDLSFPLPAKCSDP-VVVG 124
            +G+ P  + H+ W+A  K L      TEALGLD +LR RLP L+F LP K S P VVVG
Sbjct: 150 SNGVTPYAMTHKNWRAYCKTLKTFQPTTEALGLDVSLRARLPPLTFSLPCKFSSPAVVVG 209

Query: 125 KWYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGG-GERGVDFDVSVEREVI 184
           KWYCPFIFVR+G+V SQ+ NSPYYEM LQQNW E+FGCGN    G RGV+ DV VE+EV+
Sbjct: 210 KWYCPFIFVREGDVDSQIKNSPYYEMALQQNWVEVFGCGNNNNSGGRGVELDVLVEKEVV 269

Query: 185 SIAGQRADGREVGDGVVWFGSRGVGLSLAIEERVKWEEERAGFEFGKEKEEKYVKMNRRE 244
           S+ G+   G  +GDG  WFGS  VGLS+AI ER++WEEER GFE+ +E  EK VK+ RRE
Sbjct: 270 SVGGRAVGGVNIGDGAAWFGSSRVGLSMAIVERIRWEEERGGFEWVREGGEKVVKVRRRE 329

Query: 245 DFGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKW 287
           +F GV  WRRFGCYVLVERF LKRMDGSLVL+WEFRHTHQIRTKW
Sbjct: 330 EFDGVGMWRRFGCYVLVERFELKRMDGSLVLSWEFRHTHQIRTKW 368

BLAST of MC09g_new0448 vs. TAIR 10
Match: AT1G13530.1 (Protein of unknown function (DUF1262) )

HSP 1 Score: 221.5 bits (563), Expect = 8.9e-58
Identity = 127/295 (43.05%), Postives = 173/295 (58.64%), Query Frame = 0

Query: 6   AYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVSKSVAP 65
           A  S+KE++   CC C   +++  P+L DP + YQQFEI  R    G     F + SVA 
Sbjct: 109 ACVSAKEEDKVSCCLCFTYVSNATPRLLDPFDIYQQFEIHQR----GSSTRKFFATSVAS 168

Query: 66  DGIPPNFLRHEGWKAGTKPLNKNLE---LTEALG-LDAALRGRLPDLSFPLPAKCSDPVV 125
           DGIPP FLR +GW   T P + + +   + +A G +DA LR  LPD            VV
Sbjct: 169 DGIPPRFLRRKGW---TVPFSASQDYGLIDDAKGVVDAKLRYELPDF--------GKSVV 228

Query: 126 VGKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERGVDFDVSVEREV 185
           VGKWY PF+FV++G+   Q+  S YY MTL Q +EE+F C N+      V  DV VE EV
Sbjct: 229 VGKWYVPFLFVKEGDAKDQMKKSMYYSMTLHQRFEEVFFCENVDNKNCEVIVDVEVETEV 288

Query: 186 ISIAGQRADGREV----GDGVVWFG---SRGVGLSLAIEERVKWEEERAGFEFGKEKEEK 245
           + + G+   GRE      DGVVWFG   ++ +G+   + ER+KWEEER  F + K+  E 
Sbjct: 289 VKLEGENI-GRETKGVNSDGVVWFGVSETQKIGIGSVVLERMKWEEER--FGWSKKGNEL 348

Query: 246 YVKMNRREDF-GGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKWN 289
              + R E F GG  +W+ + CYVLVE F LK+ DGSLVLT+EFRH  ++++KW+
Sbjct: 349 KSSIKRTEKFEGGGPQWKSYRCYVLVETFELKKTDGSLVLTYEFRHVDKLKSKWD 385

BLAST of MC09g_new0448 vs. TAIR 10
Match: AT1G13520.1 (Protein of unknown function (DUF1262) )

HSP 1 Score: 216.1 bits (549), Expect = 3.7e-56
Identity = 118/294 (40.14%), Postives = 172/294 (58.50%), Query Frame = 0

Query: 6   AYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVSKSVAP 65
           A  S+KE++   CC+C   + + +PQ  DP + YQQFE+  R    G     + + SVAP
Sbjct: 109 ASASAKEEDRVPCCFCFSYVPEVKPQEADPYDIYQQFEVHQRKPSSG----YYTATSVAP 168

Query: 66  DGIPPNFLRHEGWKAGTKPLNKNLELT-EALGLDAALRGRLPDLSFPLPAKCSDPVVVGK 125
           +GIPP FL+ + W       +++  LT +A G++  LR +LP+         +  VVVGK
Sbjct: 169 NGIPPEFLKRKYWTVEYSN-SQDFGLTDDAKGINTTLRSKLPN-------DMNTSVVVGK 228

Query: 126 WYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERG--VDFDVSVEREVI 185
           WY PFIFV++ +   Q+ +SPYY MTL+Q WEE++ C N+   ++G  V  DV VE +V+
Sbjct: 229 WYVPFIFVKERDAKDQIKSSPYYSMTLKQRWEEVYSCVNVNYNQKGGEVVVDVDVETQVV 288

Query: 186 SIAGQRADGREV-GDGVVWFGSRG-------VGLSLAIEERVKWEEERAGFEFGKEKEEK 245
            + GQ    RE  G G VWF   G       +GL   + ER+KWEEER G+    E+   
Sbjct: 289 KLEGQETISRETSGGGFVWFSVLGDERQDKKIGLGSVVVERMKWEEERFGWLNNGERSN- 348

Query: 246 YVKMNRREDFGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKWN 289
            +K + R + GG   W+ + C VL+E F LKRMDGSLVLT+EF H  ++++KW+
Sbjct: 349 -IKRSERFE-GGSSHWKSYRCLVLIESFELKRMDGSLVLTYEFTHVDKLKSKWD 387

BLAST of MC09g_new0448 vs. TAIR 10
Match: AT1G13540.1 (Protein of unknown function (DUF1262) )

HSP 1 Score: 212.2 bits (539), Expect = 5.4e-55
Identity = 117/284 (41.20%), Postives = 160/284 (56.34%), Query Frame = 0

Query: 9   SSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWG--FVSKSVAPD 68
           S+KED++  CC+C   + + +P+  DP + YQQF+I  +      +P    + + SVAPD
Sbjct: 113 SAKEDDIVSCCFCSSFVPEAKPKQLDPFDMYQQFKIHQK------EPSSRCYYATSVAPD 172

Query: 69  GIPPNFLRHEGWKAGTKPLNKNLELTEALGLDAALRGRLPDLSFPLPAKCSDPVVVGKWY 128
           G+PP FLR + W              +A GL   LR  LP L            VVGKWY
Sbjct: 173 GVPPWFLRKKEWTVEYSRFQDFELRDDAKGLITELRTELPLLGM--------STVVGKWY 232

Query: 129 CPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGERGVDFDVSVEREVISIAG 188
            PFIFV++ EV  Q+ NS YY MTL+Q W+E+F   N     R V  DV VE EV+ + G
Sbjct: 233 VPFIFVKEREVKDQIKNSMYYSMTLEQRWKELFSDENDKSENRDVVVDVEVETEVVKLEG 292

Query: 189 QRADGREVGDGVVWF--GSRGVGLSLAIEERVKWEEERAGFEFGKEKEEKYVKMNRREDF 248
           Q  +     +G VWF  G + +GL   + ER+KWEEER G+  GK   E+ + + R E  
Sbjct: 293 QMIERGLEANGFVWFGVGDKKIGLGSVVVERMKWEEERFGWT-GKGDNERSMTVKRLEKS 352

Query: 249 GGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKWN 289
                W+ + CYVL+E FVLKRMD SLVLT+EF H  +++TKW+
Sbjct: 353 TDGSLWKHYHCYVLIESFVLKRMDESLVLTYEFTHVDKLKTKWD 381

BLAST of MC09g_new0448 vs. TAIR 10
Match: AT1G13500.1 (Protein of unknown function (DUF1262) )

HSP 1 Score: 209.1 bits (531), Expect = 4.6e-54
Identity = 122/293 (41.64%), Postives = 167/293 (57.00%), Query Frame = 0

Query: 6   AYTSSKEDEMSRCCWCCFVINDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVSKSVAP 65
           A  S+KE + + CC+C   I +  P+  DP +  QQFEI     +       F + SVA 
Sbjct: 107 ACVSAKEGDRASCCFCFTYIPEAVPRPLDPFDINQQFEIH----QGRSSTKKFFATSVAS 166

Query: 66  DGIPPNFLRHEGWKA--GTKPLNKNLELTEALGL-DAALRGRLPDLSFPLPAKCSDPVVV 125
           DGIPP FL  +GW        +     + +A G+ DA LR  LPDL         + VVV
Sbjct: 167 DGIPPQFLTRKGWSVIFSAPEVEDYGLIDDAKGIVDAKLRYELPDL--------DNSVVV 226

Query: 126 GKWYCPFIFVRDGEVGSQVSNSPYYEMTLQQNWEEIFGCGNLGGGER-GVDFDVSVEREV 185
           GKWY PF+FV++G+   Q+  S YY MTLQQ +EE+F C N+G  +R  V  DV VE EV
Sbjct: 227 GKWYVPFLFVKEGDAKDQMYKSMYYSMTLQQRFEEVFFCENVGNNKRFEVVVDVEVETEV 286

Query: 186 ISIAGQRADGREVG---DGVVWFGSRG---VGLSLAIEERVKWEEERAGFEFGKEKEEKY 245
           + + G+R      G   DGVVWF + G   +GL   + ER+KWEEER G+     ++   
Sbjct: 287 VKLEGERIARETQGVNSDGVVWFNASGNEKIGLGSVVLERMKWEEERFGWLNKGYEQRSS 346

Query: 246 VKMNRREDFGGVEEWRRFGCYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKWN 289
           +K   R + GG   W+ + CYVLVE F LKR DGSLVLT+EF+H  ++++KW+
Sbjct: 347 IKRTERFE-GGGPYWKSYRCYVLVETFELKRTDGSLVLTYEFKHVDKLKSKWD 386

BLAST of MC09g_new0448 vs. TAIR 10
Match: AT1G13490.1 (Protein of unknown function (DUF1262) )

HSP 1 Score: 208.8 bits (530), Expect = 6.0e-54
Identity = 121/274 (44.16%), Postives = 162/274 (59.12%), Query Frame = 0

Query: 25  INDKRPQLFDPKNAYQQFEISSRIIECGGQPWGFVSKSVAPDGIPPNFLRHEGWKAGTKP 84
           I D  P+  DP + YQQFEI  R    G     F + SVA +GIPP FL  +GW A + P
Sbjct: 105 IPDADPRPLDPFDIYQQFEIHQR----GSSNQKFFATSVASEGIPPQFLTRKGWVAASLP 164

Query: 85  LNKNLELTEALG-LDAALRGRLPDLSFPLPAKCSDPVVVGKWYCPFIFVRD-GEVGSQVS 144
             +   + +A G +DA LR  LPDL           VVVGKWY PF+FV++ G+   Q++
Sbjct: 165 FEEYGLIDDAKGVVDAKLRYELPDL--------DKSVVVGKWYVPFLFVKEGGDANDQMN 224

Query: 145 NSPYYEMTLQQNWEEIFGCGNLGGGER-GVDFDVSVEREVISIAGQRADGREVG---DGV 204
            S YY MTLQQ +EE+F C N+G  +   V  DV VE EV+ + G +      G   DGV
Sbjct: 225 KSMYYSMTLQQRFEEVFFCENIGNNKHFEVVVDVEVETEVVKLEGDKIARETKGVNSDGV 284

Query: 205 VWF---GSRGVGLSLAIEERVKWEEERAGFEFGKEKEEKYVKMNRREDF-GGVEEWRRFG 264
           VWF   G++ +GL   + ER+KWEEER G+    +K+E    + R E F GG   W+ + 
Sbjct: 285 VWFSVSGTQKIGLGSVVLERMKWEEERFGW---SKKDELRSSIKRTEKFEGGGPHWKSYR 344

Query: 265 CYVLVERFVLKRMDGSLVLTWEFRHTHQIRTKWN 289
           CYVLVE F LKR DGSLVLT+EF+H  ++++KW+
Sbjct: 345 CYVLVETFELKRTDGSLVLTYEFKHVDKLKSKWD 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_022149022.14.39e-21999.65uncharacterized protein LOC111017542 [Momordica charantia][more]
XP_022149086.17.59e-16280.28uncharacterized protein LOC111017584 [Momordica charantia][more]
XP_022149112.11.63e-14376.06uncharacterized protein LOC111017603 [Momordica charantia][more]
KAG6574010.11.75e-11764.44hypothetical protein SDJN03_27897, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022945417.11.00e-11664.44uncharacterized protein LOC111449654 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1D6P22.12e-21999.65uncharacterized protein LOC111017542 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1D7B13.67e-16280.28uncharacterized protein LOC111017584 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1D6Y47.89e-14476.06uncharacterized protein LOC111017603 OS=Momordica charantia OX=3673 GN=LOC111017... [more]
A0A6J1G0V44.86e-11764.44uncharacterized protein LOC111449654 OS=Cucurbita moschata OX=3662 GN=LOC1114496... [more]
A0A5A7SZ712.68e-11361.05Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
AT1G13530.18.9e-5843.05Protein of unknown function (DUF1262) [more]
AT1G13520.13.7e-5640.14Protein of unknown function (DUF1262) [more]
AT1G13540.15.4e-5541.20Protein of unknown function (DUF1262) [more]
AT1G13500.14.6e-5441.64Protein of unknown function (DUF1262) [more]
AT1G13490.16.0e-5444.16Protein of unknown function (DUF1262) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008872Insecticidal crystal toxinPFAMPF05431Toxin_10coord: 120..287
e-value: 5.9E-8
score: 32.6
NoneNo IPR availablePANTHERPTHR31050:SF14SUBFAMILY NOT NAMEDcoord: 4..288
NoneNo IPR availablePANTHERPTHR31050OS08G0413200 PROTEINcoord: 4..288

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g_new0448.1MC09g_new0448.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035821 modulation of process of other organism
molecular_function GO:0090729 toxin activity