MC09g_new0426 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g_new0426
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 family protein
Location	MC09: 19874203 .. 19875306 (+)
RNA-Seq Expression	MC09g_new0426
Synteny	MC09g_new0426

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGAATTGAAATTCGGAGAAGAATTAGGAAAGAAATCCCCAGACCTAAACCCGCTGCCATTAAATACCTTCCTTTAATCTCTGATCGTCTCCAAAGAATACCTAAATTCTCTCTGTAGAATCTACATTACTCTCTCTCGCTTCTTCAATTTCCTTTCGCCGATCGATCGCGTTTTCTCTTGTTGTTGAATCGATTAAGTTGCGCGATGAGGAGGATTCTGCTGAAGACTTGTGATAACCAGATTTACGAAGTGGAGGAGGCGATCGCGAAGCAATCCGGAGTGATTCGTAACTTTCTGGAAGATTTCGATGGCGATTGCGTCGAGATTCCGCTGCTGAAAGTGAACGAACGAATGCTGAAGGTGGTAATGGAGTGGTGCGCGATGCACGCCGGGGAGGAATTGGAGGAGAAGGAACTCCAAGATTGGGAGAGCAAGTTCGTGGCGATGGACGATCTTGATTTTCTTTACGAGGTACTGATGGCGGCGAATTACCTAGAAGTGGTGGATTTGGTCCATCGCCTTTGCAAGAAAGTTGCCGACATCATCGCCGGAAAGCAGCCGGAGGAAATTCGGAAGATCTTTAACATTGAGAATGATTTCACGCCGGAAGAGGAGGCGGAGCTCCGGAAGCGGAATGCTTGGACTCTGGATCCCAAAAACAAGTAGTATCTTATTCTTGCTGCATTTGCTTCTGAATTCTGATGGAATTCTTTTTTCTTTCTTTAATTTCTTTTCTTCTTGATCTTCATGAGTTAGGAGATGGAATTCTTTAGTAGATTTTTTATTTTAGTGATTTTTCTAACTCTAGTCGATGATGTCAAAATTTCAATACTGATGGAATATTCTAATATGTTACGACACTAAATCTTAGATCAAAGAAAAGTGTAATTTGCCCAAGAATTAATATGGATATAAATAAATAAAGTAATATTAAACTTGTTATTTTAAAATTGGGCTTAGAAAAATTGTCCAAAGGGCCATCTCAACCCAATGGCAATAATACATAAACTCAAGCCCTTAAAAAATAATATACTACATGGACAGACATTATGTATATTAACTATAAAAATAAACAATAAAATTCATTCCAATATTTTTTATAT

mRNA sequence

ATGAGGAGGATTCTGCTGAAGACTTGTGATAACCAGATTTACGAAGTGGAGGAGGCGATCGCGAAGCAATCCGGAGTGATTCGTAACTTTCTGGAAGATTTCGATGGCGATTGCGTCGAGATTCCGCTGCTGAAAGTGAACGAACGAATGCTGAAGGTGGTAATGGAGTGGTGCGCGATGCACGCCGGGGAGGAATTGGAGGAGAAGGAACTCCAAGATTGGGAGAGCAAGTTCGTGGCGATGGACGATCTTGATTTTCTTTACGAGGTACTGATGGCGGCGAATTACCTAGAAGTGGTGGATTTGGTCCATCGCCTTTGCAAGAAAGTTGCCGACATCATCGCCGGAAAGCAGCCGGAGGAAATTCGGAAGATCTTTAACATTGAGAATGATTTCACGCCGGAAGAGGAGGCGGAGCTCCGGAAGCGGAATGCTTGGACTCTGGATCCCAAAAACAAGTAG

Coding sequence (CDS)

ATGAGGAGGATTCTGCTGAAGACTTGTGATAACCAGATTTACGAAGTGGAGGAGGCGATCGCGAAGCAATCCGGAGTGATTCGTAACTTTCTGGAAGATTTCGATGGCGATTGCGTCGAGATTCCGCTGCTGAAAGTGAACGAACGAATGCTGAAGGTGGTAATGGAGTGGTGCGCGATGCACGCCGGGGAGGAATTGGAGGAGAAGGAACTCCAAGATTGGGAGAGCAAGTTCGTGGCGATGGACGATCTTGATTTTCTTTACGAGGTACTGATGGCGGCGAATTACCTAGAAGTGGTGGATTTGGTCCATCGCCTTTGCAAGAAAGTTGCCGACATCATCGCCGGAAAGCAGCCGGAGGAAATTCGGAAGATCTTTAACATTGAGAATGATTTCACGCCGGAAGAGGAGGCGGAGCTCCGGAAGCGGAATGCTTGGACTCTGGATCCCAAAAACAAGTAG

Protein sequence

MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPEEIRKIFNIENDFTPEEEAELRKRNAWTLDPKNK

Homology

[Close]

BLAST of MC09g_new0426 vs. ExPASy Swiss-Prot
Match: B8MDP8 (E3 ubiquitin ligase complex SCF subunit sconC OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) OX=441959 GN=sconC PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 3.4e-29
Identity = 67/157 (42.68%), Postives = 101/157 (64.33%), Query Frame = 0


Query: 3   RILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAMH- 62
           ++ L++ D     VE A+A++S +I+N LED       +P+  VNE +LK V+EWC  H 
Sbjct: 4   QVTLQSSDQVNITVERAVAERSMLIKNLLEDLGESEEPVPIPNVNESVLKKVIEWCTHHK 63

Query: 63  -----AGEELEEK----ELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVAD 122
                 GEE + +    E+ +W+ KF+ +D  + L+E+++AANYL++  L+   CK VA+
Sbjct: 64  NDPQTTGEEDDNRRRTTEIDEWDQKFMQVDQ-EMLFEIILAANYLDIKALLDVGCKTVAN 123

Query: 123 IIAGKQPEEIRKIFNIENDFTPEEEAELRKRNAWTLD 150
           +I GK PEEIRK FNI+NDFTPEEE ++R+ N W  D
Sbjct: 124 MIKGKSPEEIRKTFNIQNDFTPEEEDQIRRENEWAED 159

[Close]

BLAST of MC09g_new0426 vs. ExPASy Swiss-Prot
Match: B6QGB9 (E3 ubiquitin ligase complex SCF subunit sconC OS=Talaromyces marneffei (strain ATCC 18224 / CBS 334.59 / QM 7333) OX=441960 GN=sconC PE=3 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 5.9e-29
Identity = 66/157 (42.04%), Postives = 102/157 (64.97%), Query Frame = 0


Query: 3   RILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAMH- 62
           ++ L++ D+    VE A+A++S +I+N LED       +P+  VNE +LK V+EWC  H 
Sbjct: 4   QVTLQSSDSVDITVERAVAERSMLIKNLLEDLGESEEPVPIPNVNESVLKKVIEWCTHHK 63

Query: 63  -----AGEELEEK----ELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVAD 122
                 GE+ + +    E+ +W+ KF+ +D  + L+E+++AANYL++  L+   CK VA+
Sbjct: 64  NDPQSTGEDDDNRRRTTEIDEWDQKFMQVDQ-EMLFEIILAANYLDIKALLDVGCKTVAN 123

Query: 123 IIAGKQPEEIRKIFNIENDFTPEEEAELRKRNAWTLD 150
           +I GK PEEIRK FNI+NDFTPEEE ++R+ N W  D
Sbjct: 124 MIKGKSPEEIRKTFNIQNDFTPEEEDQIRRENEWAED 159

[Close]

BLAST of MC09g_new0426 vs. ExPASy Swiss-Prot
Match: O81057 (SKP1-like protein 14 OS=Arabidopsis thaliana OX=3702 GN=ASK14 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.7e-28
Identity = 65/149 (43.62%), Postives = 99/149 (66.44%), Query Frame = 0


Query: 3   RILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCV--EIPLLKVNERMLKVVMEWCAM 62
           +I+L + D + +EVEEA+A++  ++ + +ED   DCV  E+PL  V  ++L +V+E+C  
Sbjct: 5   KIVLSSSDGESFEVEEAVARKLKIVEHMIED---DCVVTEVPLQNVTGKILSIVVEYCKK 64

Query: 63  HAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPE 122
           H  +E E  E + W+ +F+   D   ++++L+AANYL +  L+    + VAD I  K PE
Sbjct: 65  HVVDE-ESDEFKTWDEEFMKKFDQPTVFQLLLAANYLNIKGLLDLSAQTVADRIKDKTPE 124

Query: 123 EIRKIFNIENDFTPEEEAELRKRNAWTLD 150
           EIR+IFNIENDFTPEEEA +RK NAW  +
Sbjct: 125 EIREIFNIENDFTPEEEAAVRKENAWAFE 149

[Close]

BLAST of MC09g_new0426 vs. ExPASy Swiss-Prot
Match: Q9Y709 (Suppressor of kinetochore protein 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=skp1 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.7e-28
Identity = 68/161 (42.24%), Postives = 103/161 (63.98%), Query Frame = 0


Query: 1   MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAM 60
           M +I L + DN+ + V++ IA++S +I+N LED     V IPL  V+  +L+ V+EWC  
Sbjct: 1   MSKIKLISSDNEEFVVDQLIAERSMLIKNMLEDVGEINVPIPLPNVSSNVLRKVLEWCEH 60

Query: 61  H-----AGEELE-------EKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCK 120
           H     +G E E         ++ +W+ KF+A+D  + L+E+++A+NYL++  L+   CK
Sbjct: 61  HKNDLYSGTEEESDIRLKKSTDIDEWDRKFMAVDQ-EMLFEIVLASNYLDIKPLLDTGCK 120

Query: 121 KVADIIAGKQPEEIRKIFNIENDFTPEEEAELRKRNAWTLD 150
            VA++I GK PE+IRK FNI NDFTPEEE ++RK N W  D
Sbjct: 121 TVANMIRGKSPEDIRKTFNIPNDFTPEEEEQIRKENEWAED 160

[Close]

BLAST of MC09g_new0426 vs. ExPASy Swiss-Prot
Match: O49484 (SKP1-like protein 11 OS=Arabidopsis thaliana OX=3702 GN=ASK11 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.2e-28
Identity = 68/153 (44.44%), Postives = 99/153 (64.71%), Query Frame = 0


Query: 2   RRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVE--IPLLKVNERMLKVVMEWCA 61
           + I+L + D Q +EVEEA+A QS  I + +ED   DCV   IPL  V  ++L  V+E+C 
Sbjct: 4   KMIVLMSSDGQSFEVEEAVAIQSQTIAHMVED---DCVADGIPLANVESKILVKVIEYCK 63

Query: 62  MHAGEE---LEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAG 121
            H  +E   + E++L +W+ KF+ ++    ++E+++AANYL +  L+   C+ VAD+I G
Sbjct: 64  KHHVDEANPISEEDLNNWDEKFMDLEQ-STIFELILAANYLNIKSLLDLTCQTVADMIKG 123

Query: 122 KQPEEIRKIFNIENDFTPEEEAELRKRNAWTLD 150
           K PEEIR  FNIENDFTPEEE  +RK N W  +
Sbjct: 124 KTPEEIRSTFNIENDFTPEEEEAVRKENQWAFE 152

[Close]

BLAST of MC09g_new0426 vs. NCBI nr
Match: XP_022139312.1 (SKP1-like protein 14 [Momordica charantia])

HSP 1 Score: 307 bits (786), Expect = 2.91e-105
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 0


Query: 1   MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAM 60
           MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAM
Sbjct: 1   MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAM 60

Query: 61  HAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPE 120
           HAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPE
Sbjct: 61  HAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPE 120

Query: 121 EIRKIFNIENDFTPEEEAELRKRNAWTLDPKNK 153
           EIRKIFNIENDFTPEEEAELRKRNAWTLDPKNK
Sbjct: 121 EIRKIFNIENDFTPEEEAELRKRNAWTLDPKNK 153

[Close]

BLAST of MC09g_new0426 vs. NCBI nr
Match: XP_008446842.1 (PREDICTED: SKP1-like protein 12 [Cucumis melo])

HSP 1 Score: 172 bits (435), Expect = 1.14e-51
Identity = 84/152 (55.26%), Postives = 117/152 (76.97%), Query Frame = 0


Query: 1   MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLED--FDGDCVEIPLLKVNERMLKVVMEWC 60
           MR + L++ +N+I++V E +AKQS V+RNFLE+   +GD + IPL  ++ ++L +V+EW 
Sbjct: 1   MRTVNLRSSENEIFKVSEEVAKQSVVVRNFLEEDNSNGDEITIPLPNISGKLLGMVIEWI 60

Query: 61  AMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQ 120
           AMH  E+L E++L  W++KF+   DLD L+E++MAANYLEV DL H  C+ VAD I+GK 
Sbjct: 61  AMHVEEKLAEEDLHAWKTKFMEDLDLDLLFELIMAANYLEVTDLFHATCQCVADKISGKS 120

Query: 121 PEEIRKIFNIENDFTPEEEAELRKRNAWTLDP 150
           PEEIRKIFNI NDFTPEEEAE+R++NAW  +P
Sbjct: 121 PEEIRKIFNITNDFTPEEEAEIRRQNAWVFEP 152

[Close]

BLAST of MC09g_new0426 vs. NCBI nr
Match: XP_038892847.1 (SKP1-like protein 14 [Benincasa hispida])

HSP 1 Score: 169 bits (428), Expect = 1.28e-50
Identity = 85/151 (56.29%), Postives = 113/151 (74.83%), Query Frame = 0


Query: 1   MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDG--DCVEIPLLKVNERMLKVVMEWC 60
           MR + L++ +N++++V E IAKQS V+RNFLE+ +   D V IPL  ++ R+L +V+EW 
Sbjct: 1   MRTVNLRSSENEVFKVREEIAKQSVVVRNFLEEDESNSDEVTIPLPNISGRILAMVIEWI 60

Query: 61  AMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQ 120
             H  E+L E  L+DW+ KFV   DLD L+E++MAANYLEV DL H+ C+ VAD IAGK 
Sbjct: 61  VKHVEEKLTETALKDWQIKFVGDLDLDSLFELIMAANYLEVTDLFHQTCQSVADKIAGKS 120

Query: 121 PEEIRKIFNIENDFTPEEEAELRKRNAWTLD 149
           PEEIRKIFNI NDFTPEEEAE+R++NAW  +
Sbjct: 121 PEEIRKIFNITNDFTPEEEAEIRRQNAWVFE 151

[Close]

BLAST of MC09g_new0426 vs. NCBI nr
Match: KAA0034704.1 (SKP1-like protein 12 [Cucumis melo var. makuwa])

HSP 1 Score: 172 bits (435), Expect = 2.40e-50
Identity = 84/152 (55.26%), Postives = 117/152 (76.97%), Query Frame = 0


Query: 1   MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLED--FDGDCVEIPLLKVNERMLKVVMEWC 60
           MR + L++ +N+I++V E +AKQS V+RNFLE+   +GD + IPL  ++ ++L +V+EW 
Sbjct: 1   MRTVNLRSSENEIFKVSEEVAKQSVVVRNFLEEDNSNGDEITIPLPNISGKLLGMVIEWI 60

Query: 61  AMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQ 120
           AMH  E+L E++L  W++KF+   DLD L+E++MAANYLEV DL H  C+ VAD I+GK 
Sbjct: 61  AMHVEEKLAEEDLHAWKTKFMEDLDLDLLFELIMAANYLEVTDLFHATCQCVADKISGKS 120

Query: 121 PEEIRKIFNIENDFTPEEEAELRKRNAWTLDP 150
           PEEIRKIFNI NDFTPEEEAE+R++NAW  +P
Sbjct: 121 PEEIRKIFNITNDFTPEEEAEIRRQNAWVFEP 152

[Close]

BLAST of MC09g_new0426 vs. NCBI nr
Match: TYK09255.1 (SKP1-like protein 12 [Cucumis melo var. makuwa])

HSP 1 Score: 172 bits (435), Expect = 2.54e-50
Identity = 84/152 (55.26%), Postives = 117/152 (76.97%), Query Frame = 0


Query: 1   MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLED--FDGDCVEIPLLKVNERMLKVVMEWC 60
           MR + L++ +N+I++V E +AKQS V+RNFLE+   +GD + IPL  ++ ++L +V+EW 
Sbjct: 1   MRTVNLRSSENEIFKVSEEVAKQSVVVRNFLEEDNSNGDEITIPLPNISGKLLGMVIEWI 60

Query: 61  AMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQ 120
           AMH  E+L E++L  W++KF+   DLD L+E++MAANYLEV DL H  C+ VAD I+GK 
Sbjct: 61  AMHVEEKLAEEDLHAWKTKFMEDLDLDLLFELIMAANYLEVTDLFHATCQCVADKISGKS 120

Query: 121 PEEIRKIFNIENDFTPEEEAELRKRNAWTLDP 150
           PEEIRKIFNI NDFTPEEEAE+R++NAW  +P
Sbjct: 121 PEEIRKIFNITNDFTPEEEAEIRRQNAWVFEP 152

[Close]

BLAST of MC09g_new0426 vs. ExPASy TrEMBL
Match: A0A6J1CDL6 (SKP1-like protein OS=Momordica charantia OX=3673 GN=LOC111010258 PE=3 SV=1)

HSP 1 Score: 307 bits (786), Expect = 1.41e-105
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 0


Query: 1   MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAM 60
           MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAM
Sbjct: 1   MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVEIPLLKVNERMLKVVMEWCAM 60

Query: 61  HAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPE 120
           HAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPE
Sbjct: 61  HAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPE 120

Query: 121 EIRKIFNIENDFTPEEEAELRKRNAWTLDPKNK 153
           EIRKIFNIENDFTPEEEAELRKRNAWTLDPKNK
Sbjct: 121 EIRKIFNIENDFTPEEEAELRKRNAWTLDPKNK 153

[Close]

BLAST of MC09g_new0426 vs. ExPASy TrEMBL
Match: A0A1S3BGP6 (SKP1-like protein OS=Cucumis melo OX=3656 GN=LOC103489446 PE=3 SV=1)

HSP 1 Score: 172 bits (435), Expect = 5.53e-52
Identity = 84/152 (55.26%), Postives = 117/152 (76.97%), Query Frame = 0


Query: 1   MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLED--FDGDCVEIPLLKVNERMLKVVMEWC 60
           MR + L++ +N+I++V E +AKQS V+RNFLE+   +GD + IPL  ++ ++L +V+EW 
Sbjct: 1   MRTVNLRSSENEIFKVSEEVAKQSVVVRNFLEEDNSNGDEITIPLPNISGKLLGMVIEWI 60

Query: 61  AMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQ 120
           AMH  E+L E++L  W++KF+   DLD L+E++MAANYLEV DL H  C+ VAD I+GK 
Sbjct: 61  AMHVEEKLAEEDLHAWKTKFMEDLDLDLLFELIMAANYLEVTDLFHATCQCVADKISGKS 120

Query: 121 PEEIRKIFNIENDFTPEEEAELRKRNAWTLDP 150
           PEEIRKIFNI NDFTPEEEAE+R++NAW  +P
Sbjct: 121 PEEIRKIFNITNDFTPEEEAEIRRQNAWVFEP 152

[Close]

BLAST of MC09g_new0426 vs. ExPASy TrEMBL
Match: A0A5A7SU15 (SKP1-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007240 PE=3 SV=1)

HSP 1 Score: 172 bits (435), Expect = 1.16e-50
Identity = 84/152 (55.26%), Postives = 117/152 (76.97%), Query Frame = 0


Query: 1   MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLED--FDGDCVEIPLLKVNERMLKVVMEWC 60
           MR + L++ +N+I++V E +AKQS V+RNFLE+   +GD + IPL  ++ ++L +V+EW 
Sbjct: 1   MRTVNLRSSENEIFKVSEEVAKQSVVVRNFLEEDNSNGDEITIPLPNISGKLLGMVIEWI 60

Query: 61  AMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQ 120
           AMH  E+L E++L  W++KF+   DLD L+E++MAANYLEV DL H  C+ VAD I+GK 
Sbjct: 61  AMHVEEKLAEEDLHAWKTKFMEDLDLDLLFELIMAANYLEVTDLFHATCQCVADKISGKS 120

Query: 121 PEEIRKIFNIENDFTPEEEAELRKRNAWTLDP 150
           PEEIRKIFNI NDFTPEEEAE+R++NAW  +P
Sbjct: 121 PEEIRKIFNITNDFTPEEEAEIRRQNAWVFEP 152

[Close]

BLAST of MC09g_new0426 vs. ExPASy TrEMBL
Match: A0A5D3CCN6 (SKP1-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002490 PE=3 SV=1)

HSP 1 Score: 172 bits (435), Expect = 1.23e-50
Identity = 84/152 (55.26%), Postives = 117/152 (76.97%), Query Frame = 0


Query: 1   MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLED--FDGDCVEIPLLKVNERMLKVVMEWC 60
           MR + L++ +N+I++V E +AKQS V+RNFLE+   +GD + IPL  ++ ++L +V+EW 
Sbjct: 1   MRTVNLRSSENEIFKVSEEVAKQSVVVRNFLEEDNSNGDEITIPLPNISGKLLGMVIEWI 60

Query: 61  AMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQ 120
           AMH  E+L E++L  W++KF+   DLD L+E++MAANYLEV DL H  C+ VAD I+GK 
Sbjct: 61  AMHVEEKLAEEDLHAWKTKFMEDLDLDLLFELIMAANYLEVTDLFHATCQCVADKISGKS 120

Query: 121 PEEIRKIFNIENDFTPEEEAELRKRNAWTLDP 150
           PEEIRKIFNI NDFTPEEEAE+R++NAW  +P
Sbjct: 121 PEEIRKIFNITNDFTPEEEAEIRRQNAWVFEP 152

[Close]

BLAST of MC09g_new0426 vs. ExPASy TrEMBL
Match: A0A6J1H051 (SKP1-like protein OS=Cucurbita moschata OX=3662 GN=LOC111458404 PE=3 SV=1)

HSP 1 Score: 168 bits (425), Expect = 1.83e-50
Identity = 83/152 (54.61%), Postives = 116/152 (76.32%), Query Frame = 0


Query: 1   MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDF--DGDCVEIPLLKVNERMLKVVMEWC 60
           MR   LK+ DN+I++V E IA QS VI++FLEDF  D D + IPL  V+ R+L++V+EW 
Sbjct: 1   MRTFQLKSSDNKIFKVSEEIAIQSAVIKSFLEDFGSDSDEIVIPLPNVSGRILEIVIEWI 60

Query: 61  AMHAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQ 120
             HA +EL ++E+QDWE+KF+   +++   E++MAANYLEV+ L+HR C+ +AD I+GK 
Sbjct: 61  VKHADDELTKEEVQDWENKFMNDLNMEVTTELVMAANYLEVIGLLHRTCQSIADQISGKS 120

Query: 121 PEEIRKIFNIENDFTPEEEAELRKRNAWTLDP 150
           PEEIR+I NI NDFTPEEEAE+R++N W L+P
Sbjct: 121 PEEIRRILNITNDFTPEEEAEIREQNGWDLNP 152

[Close]

BLAST of MC09g_new0426 vs. TAIR 10
Match: AT2G03170.1 (SKP1-like 14 )

HSP 1 Score: 127.1 bits (318), Expect = 1.2e-29
Identity = 65/149 (43.62%), Postives = 99/149 (66.44%), Query Frame = 0


Query: 3   RILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCV--EIPLLKVNERMLKVVMEWCAM 62
           +I+L + D + +EVEEA+A++  ++ + +ED   DCV  E+PL  V  ++L +V+E+C  
Sbjct: 5   KIVLSSSDGESFEVEEAVARKLKIVEHMIED---DCVVTEVPLQNVTGKILSIVVEYCKK 64

Query: 63  HAGEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAGKQPE 122
           H  +E E  E + W+ +F+   D   ++++L+AANYL +  L+    + VAD I  K PE
Sbjct: 65  HVVDE-ESDEFKTWDEEFMKKFDQPTVFQLLLAANYLNIKGLLDLSAQTVADRIKDKTPE 124

Query: 123 EIRKIFNIENDFTPEEEAELRKRNAWTLD 150
           EIR+IFNIENDFTPEEEA +RK NAW  +
Sbjct: 125 EIREIFNIENDFTPEEEAAVRKENAWAFE 149

[Close]

BLAST of MC09g_new0426 vs. TAIR 10
Match: AT4G34210.1 (SKP1-like 11 )

HSP 1 Score: 126.7 bits (317), Expect = 1.6e-29
Identity = 68/153 (44.44%), Postives = 99/153 (64.71%), Query Frame = 0


Query: 2   RRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVE--IPLLKVNERMLKVVMEWCA 61
           + I+L + D Q +EVEEA+A QS  I + +ED   DCV   IPL  V  ++L  V+E+C 
Sbjct: 4   KMIVLMSSDGQSFEVEEAVAIQSQTIAHMVED---DCVADGIPLANVESKILVKVIEYCK 63

Query: 62  MHAGEE---LEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAG 121
            H  +E   + E++L +W+ KF+ ++    ++E+++AANYL +  L+   C+ VAD+I G
Sbjct: 64  KHHVDEANPISEEDLNNWDEKFMDLEQ-STIFELILAANYLNIKSLLDLTCQTVADMIKG 123

Query: 122 KQPEEIRKIFNIENDFTPEEEAELRKRNAWTLD 150
           K PEEIR  FNIENDFTPEEE  +RK N W  +
Sbjct: 124 KTPEEIRSTFNIENDFTPEEEEAVRKENQWAFE 152

[Close]

BLAST of MC09g_new0426 vs. TAIR 10
Match: AT4G34470.1 (SKP1-like 12 )

HSP 1 Score: 122.9 bits (307), Expect = 2.3e-28
Identity = 67/153 (43.79%), Postives = 97/153 (63.40%), Query Frame = 0


Query: 2   RRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVE--IPLLKVNERMLKVVMEWCA 61
           + I+L + D Q +EVEEA+A QS  I + +ED   DCV   IPL  V  ++L  V+E+C 
Sbjct: 4   KMIVLMSSDGQSFEVEEAVAIQSQTIAHMVED---DCVADGIPLANVESKILVKVIEYCK 63

Query: 62  MHAGEE---LEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCKKVADIIAG 121
            +  +E   + E++L  W+ KF+ ++    ++E+++AANYL +  L    C+ VAD+I G
Sbjct: 64  KYHVDEANPISEEDLNKWDEKFMDLEQ-STIFELILAANYLNIKSLFDLTCQTVADMIKG 123

Query: 122 KQPEEIRKIFNIENDFTPEEEAELRKRNAWTLD 150
           K PEEIR  FNIENDFTPEEE  +RK N W  +
Sbjct: 124 KTPEEIRSTFNIENDFTPEEEEAVRKENQWAFE 152

[Close]

BLAST of MC09g_new0426 vs. TAIR 10
Match: AT1G75950.1 (S phase kinase-associated protein 1 )

HSP 1 Score: 122.5 bits (306), Expect = 3.0e-28
Identity = 65/161 (40.37%), Postives = 101/161 (62.73%), Query Frame = 0


Query: 2   RRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVE--IPLLKVNERMLKVVMEWCA 61
           ++I+LK+ D + +EVEEA+A +S  I + +ED   DCV+  +PL  V  ++L  V+E+C 
Sbjct: 4   KKIVLKSSDGESFEVEEAVALESQTIAHMVED---DCVDNGVPLPNVTSKILAKVIEYCK 63

Query: 62  MHA-----------GEELEEKELQDWESKFVAMDDLDFLYEVLMAANYLEVVDLVHRLCK 121
            H            G    + +L+ W++ F+ +D    L+E+++AANYL + +L+   C+
Sbjct: 64  RHVEAAASKAEAVEGAATSDDDLKAWDADFMKIDQAT-LFELILAANYLNIKNLLDLTCQ 123

Query: 122 KVADIIAGKQPEEIRKIFNIENDFTPEEEAELRKRNAWTLD 150
            VAD+I GK PEEIR  FNI+NDFTPEEE E+R+ N W  +
Sbjct: 124 TVADMIKGKTPEEIRTTFNIKNDFTPEEEEEVRRENQWAFE 160

[Close]

BLAST of MC09g_new0426 vs. TAIR 10
Match: AT5G42190.1 (E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 family protein )

HSP 1 Score: 121.7 bits (304), Expect = 5.1e-28
Identity = 67/172 (38.95%), Postives = 106/172 (61.63%), Query Frame = 0


Query: 1   MRRILLKTCDNQIYEVEEAIAKQSGVIRNFLEDFDGDCVE--IPLLKVNERMLKVVMEWC 60
           +R+I LK+ D + +E++EA+A +S  I++ +ED   DC +  IPL  V  ++L  V+E+C
Sbjct: 4   VRKITLKSSDGENFEIDEAVALESQTIKHMIED---DCTDNGIPLPNVTSKILSKVIEYC 63

Query: 61  AMH--AGEELE-------------------EKELQDWESKFVAMDDLDFLYEVLMAANYL 120
             H  A E+ E                   +++L+ W+S+F+ +D    L+++++AANYL
Sbjct: 64  KRHVEAAEKSETTADAAAATTTTTVASGSSDEDLKTWDSEFIKVDQ-GTLFDLILAANYL 123

Query: 121 EVVDLVHRLCKKVADIIAGKQPEEIRKIFNIENDFTPEEEAELRKRNAWTLD 150
            +  L+   C+ VAD+I GK PEEIRK FNI+NDFTPEEE E+R+ N W  +
Sbjct: 124 NIKGLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 171

The following BLAST results are available for this feature:

BLAST of MC09g_new0426 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B8MDP8	3.4e-29	42.68	E3 ubiquitin ligase complex SCF subunit sconC OS=Talaromyces stipitatus (strain ...	[more]
B6QGB9	5.9e-29	42.04	E3 ubiquitin ligase complex SCF subunit sconC OS=Talaromyces marneffei (strain A...	[more]
O81057	1.7e-28	43.62	SKP1-like protein 14 OS=Arabidopsis thaliana OX=3702 GN=ASK14 PE=1 SV=1	[more]
Q9Y709	1.7e-28	42.24	Suppressor of kinetochore protein 1 OS=Schizosaccharomyces pombe (strain 972 / A...	[more]
O49484	2.2e-28	44.44	SKP1-like protein 11 OS=Arabidopsis thaliana OX=3702 GN=ASK11 PE=1 SV=1	[more]

BLAST of MC09g_new0426 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139312.1	2.91e-105	100.00	SKP1-like protein 14 [Momordica charantia]	[more]
XP_008446842.1	1.14e-51	55.26	PREDICTED: SKP1-like protein 12 [Cucumis melo]	[more]
XP_038892847.1	1.28e-50	56.29	SKP1-like protein 14 [Benincasa hispida]	[more]
KAA0034704.1	2.40e-50	55.26	SKP1-like protein 12 [Cucumis melo var. makuwa]	[more]
TYK09255.1	2.54e-50	55.26	SKP1-like protein 12 [Cucumis melo var. makuwa]	[more]

BLAST of MC09g_new0426 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CDL6	1.41e-105	100.00	SKP1-like protein OS=Momordica charantia OX=3673 GN=LOC111010258 PE=3 SV=1	[more]
A0A1S3BGP6	5.53e-52	55.26	SKP1-like protein OS=Cucumis melo OX=3656 GN=LOC103489446 PE=3 SV=1	[more]
A0A5A7SU15	1.16e-50	55.26	SKP1-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G...	[more]
A0A5D3CCN6	1.23e-50	55.26	SKP1-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475...	[more]
A0A6J1H051	1.83e-50	54.61	SKP1-like protein OS=Cucurbita moschata OX=3662 GN=LOC111458404 PE=3 SV=1	[more]

BLAST of MC09g_new0426 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G03170.1	1.2e-29	43.62	SKP1-like 14	[more]
AT4G34210.1	1.6e-29	44.44	SKP1-like 11	[more]
AT4G34470.1	2.3e-28	43.79	SKP1-like 12	[more]
AT1G75950.1	3.0e-28	40.37	S phase kinase-associated protein 1	[more]
AT5G42190.1	5.1e-28	38.95	E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001232	S-phase kinase-associated protein 1-like	SMART	SM00512	skp1_3	coord: 1..99 e-value: 1.7E-21 score: 87.4
IPR016897	S-phase kinase-associated protein 1	PIRSF	PIRSF028729	SCF_Skp	coord: 1..150 e-value: 1.0E-45 score: 153.3
IPR016897	S-phase kinase-associated protein 1	PANTHER	PTHR11165	SKP1	coord: 3..147
IPR016073	SKP1 component, POZ domain	PFAM	PF03931	Skp1_POZ	coord: 4..61 e-value: 2.6E-15 score: 56.3
IPR016072	SKP1 component, dimerisation	PFAM	PF01466	Skp1	coord: 106..146 e-value: 4.4E-21 score: 74.6
IPR011333	SKP1/BTB/POZ domain superfamily	GENE3D	3.30.710.10	Potassium Channel Kv1.1; Chain A	coord: 1..149 e-value: 5.2E-49 score: 168.4
IPR011333	SKP1/BTB/POZ domain superfamily	SUPERFAMILY	54695	POZ domain	coord: 3..64
IPR036296	SKP1-like, dimerisation domain superfamily	SUPERFAMILY	81382	Skp1 dimerisation domain-like	coord: 71..146

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g_new0426.1	MC09g_new0426.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0031146	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0097602	cullin family protein binding