MC09g_new0150 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g_new0150
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	mitogen-activated protein kinase kinase kinase 17-like
Location	MC09: 6299304 .. 6300200 (+)
RNA-Seq Expression	MC09g_new0150
Synteny	MC09g_new0150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGAATTGGGTTCGAGTGAATGATTTGGGTAAAGGTTCTTACGGCAAAGTGTTTTTAGCGAAACCAACGACGAGATCTCTCAAATATTTTGCTGTTAAAGTAGCTGAGCTTCAACATTATTCTTCGCTCTTGAATGAAAAACGTATACTCAAACACTTTGTCGGATTTCCAGAAATTGTTCAGTGTTTTGGGGGTGAGATGATAACGGAGGGGGATTCCTTTGATTCTGCCTTTTACAGTTTGGTATTGGAGTACGCTGCTGGAGGTACCTTAGCTGACTTGATTAGAAAACAGAAGAAGTTGTCGGAGAATACAGTGAAGGAATATCTCAAAATGATATTGAGAGGGCTATCTTGCATTCACAAACTAGGGTTTGTCCATGTCGACCTCAAGCCTGCTAACATTCTTGCATTCCCTCAAATTGATGGTAAAATGAAATTGAAGATAGCGGATTTTGGACTTTCAAGGCGATGTAACAAAAAGAGAGAGTGCAATTTCAAATTGAAGTTTAGAGGAACACCGATGTACATGTCACCAGAATCCATTTATGGTGAGGTCAACACTCCGCTTGATATTTGGTCTTTGGGTTGCATTTTGGTTGAAATGGTTACTGGAAAGCACGCATGGGACGATTGCATAGATCTCGAGCAGTTGATGTTGAAGCTTAGGGTAAATATGGAAACACCAAAAATACCCGAAGAACTATCAAAAGAAGGAAAAGACTTTCTTGAAAAATGCTTTGATCGGAATCCTAAACGACGATGGACAGCGGATATGTTACTCCAACATCCTTATCTCGAACAAGAAAAGGAAGCTATAAAGAAAGATGATGATCCAACGTACTCTTATCTGCCTCATATACTGTACAAATTTCAATCAACTATTCAAATTTGA

mRNA sequence

ATGACGAATTGGGTTCGAGTGAATGATTTGGGTAAAGGTTCTTACGGCAAAGTGTTTTTAGCGAAACCAACGACGAGATCTCTCAAATATTTTGCTGTTAAAGTAGCTGAGCTTCAACATTATTCTTCGCTCTTGAATGAAAAACGTATACTCAAACACTTTGTCGGATTTCCAGAAATTGTTCAGTGTTTTGGGGGTGAGATGATAACGGAGGGGGATTCCTTTGATTCTGCCTTTTACAGTTTGGTATTGGAGTACGCTGCTGGAGGTACCTTAGCTGACTTGATTAGAAAACAGAAGAAGTTGTCGGAGAATACAGTGAAGGAATATCTCAAAATGATATTGAGAGGGCTATCTTGCATTCACAAACTAGGGTTTGTCCATGTCGACCTCAAGCCTGCTAACATTCTTGCATTCCCTCAAATTGATGGTAAAATGAAATTGAAGATAGCGGATTTTGGACTTTCAAGGCGATGTAACAAAAAGAGAGAGTGCAATTTCAAATTGAAGTTTAGAGGAACACCGATGTACATGTCACCAGAATCCATTTATGGTGAGGTCAACACTCCGCTTGATATTTGGTCTTTGGGTTGCATTTTGGTTGAAATGGTTACTGGAAAGCACGCATGGGACGATTGCATAGATCTCGAGCAGTTGATGTTGAAGCTTAGGGTAAATATGGAAACACCAAAAATACCCGAAGAACTATCAAAAGAAGGAAAAGACTTTCTTGAAAAATGCTTTGATCGGAATCCTAAACGACGATGGACAGCGGATATGTTACTCCAACATCCTTATCTCGAACAAGAAAAGGAAGCTATAAAGAAAGATGATGATCCAACGTACTCTTATCTGCCTCATATACTGTACAAATTTCAATCAACTATTCAAATTTGA

Coding sequence (CDS)

ATGACGAATTGGGTTCGAGTGAATGATTTGGGTAAAGGTTCTTACGGCAAAGTGTTTTTAGCGAAACCAACGACGAGATCTCTCAAATATTTTGCTGTTAAAGTAGCTGAGCTTCAACATTATTCTTCGCTCTTGAATGAAAAACGTATACTCAAACACTTTGTCGGATTTCCAGAAATTGTTCAGTGTTTTGGGGGTGAGATGATAACGGAGGGGGATTCCTTTGATTCTGCCTTTTACAGTTTGGTATTGGAGTACGCTGCTGGAGGTACCTTAGCTGACTTGATTAGAAAACAGAAGAAGTTGTCGGAGAATACAGTGAAGGAATATCTCAAAATGATATTGAGAGGGCTATCTTGCATTCACAAACTAGGGTTTGTCCATGTCGACCTCAAGCCTGCTAACATTCTTGCATTCCCTCAAATTGATGGTAAAATGAAATTGAAGATAGCGGATTTTGGACTTTCAAGGCGATGTAACAAAAAGAGAGAGTGCAATTTCAAATTGAAGTTTAGAGGAACACCGATGTACATGTCACCAGAATCCATTTATGGTGAGGTCAACACTCCGCTTGATATTTGGTCTTTGGGTTGCATTTTGGTTGAAATGGTTACTGGAAAGCACGCATGGGACGATTGCATAGATCTCGAGCAGTTGATGTTGAAGCTTAGGGTAAATATGGAAACACCAAAAATACCCGAAGAACTATCAAAAGAAGGAAAAGACTTTCTTGAAAAATGCTTTGATCGGAATCCTAAACGACGATGGACAGCGGATATGTTACTCCAACATCCTTATCTCGAACAAGAAAAGGAAGCTATAAAGAAAGATGATGATCCAACGTACTCTTATCTGCCTCATATACTGTACAAATTTCAATCAACTATTCAAATTTGA

Protein sequence

MTNWVRVNDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEIVQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSCIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFKLKFRGTPMYMSPESIYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEELSKEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQEKEAIKKDDDPTYSYLPHILYKFQSTIQI

Homology

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BLAST of MC09g_new0150 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.1e-47
Identity = 117/305 (38.36%), Postives = 172/305 (56.39%), Query Frame = 0


Query: 4   WVRVNDLGKGSYGKVFLAKPTTRSLKY---FAVKVAELQHYSSLLNEKRILKHFVGFPEI 63
           WVR   +G G++  V  A  +  S  +    AVK  +    +SL NEK +L      PEI
Sbjct: 3   WVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEI 62

Query: 64  VQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRK--QKKLSENTVKEYLKMILRGL 123
           ++C+G +   E        ++L+LEYA+ G+LA  ++K   + L E+TV+ +   +LRGL
Sbjct: 63  IRCYGEDSTVEN---GEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGL 122

Query: 124 SCIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFK-LKFRGTPMY 183
             IH  GF H D+K ANIL F   DG   +KIADFGL+ R +       K ++ RGTP+Y
Sbjct: 123 RHIHAKGFAHCDIKLANILLFN--DG--SVKIADFGLAMRVDGDLTALRKSVEIRGTPLY 182

Query: 184 MSPESIY-GEVNTPLDIWSLGCILVEMVTGKHAWD--DCIDLEQLMLKLRVNMETPKIPE 243
           M+PE +   E  +  D+W+LGC +VEM +GK AW   +      L++++ V  E PKIPE
Sbjct: 183 MAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPE 242

Query: 244 ELSKEGKDFLEKCFDRNPKRRWTADMLLQHPYL-------EQEKEAIKKDDDPTYSYLPH 293
            LS+EGKDFL KCF ++P +RWTA+MLL H ++        +E   +K  D+      P 
Sbjct: 243 MLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPK 300

[Close]

BLAST of MC09g_new0150 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 163.3 bits (412), Expect = 4.2e-39
Identity = 100/270 (37.04%), Postives = 148/270 (54.81%), Query Frame = 0


Query: 4   WVRVNDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEIVQC 63
           W +   LG+G YG V+L        + FAVK  E+   +S    K ++  F    E+++ 
Sbjct: 170 WQKGQILGRGGYGSVYLGLNKDTG-ELFAVKQLEIVDINSDPKLKNMILSFSKEIEVMRS 229

Query: 64  FGGEMITE--GDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSCI 123
              + I    G S D +F S+ LEY  GG+++ L+ K    SEN +K Y K IL+GLS +
Sbjct: 230 LRHDNIVRYLGTSLDQSFLSVFLEYIPGGSISSLLGKFGAFSENVIKVYTKQILQGLSFL 289

Query: 124 HKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFKLKFRGTPMYMSPE 183
           H    +H D+K ANIL    ID K  +K++DFG S+  +      FK   +GTP +M+PE
Sbjct: 290 HANSIIHRDIKGANIL----IDTKGIVKLSDFGCSKSFS-GIVSQFK-SMQGTPYWMAPE 349

Query: 184 SI----YGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEELS 243
            I    +G  +   DIWSLGC++VEM T +  W +  +L  +M  +  +   P IP  +S
Sbjct: 350 VIKQTGHGRSS---DIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNIPSHMS 409

Query: 244 KEGKDFLEKCFDRNPKRRWTADMLLQHPYL 268
           +E  DFL  CF R+PK R  A+ LL+HP++
Sbjct: 410 QEAFDFLNLCFKRDPKERPDANQLLKHPFI 429

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BLAST of MC09g_new0150 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.7e-38
Identity = 102/269 (37.92%), Postives = 145/269 (53.90%), Query Frame = 0


Query: 4   WVRVNDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEIVQC 63
           W R   LG+GS   V+ A     S +  AVK +E+     L  E +IL   +  P ++  
Sbjct: 3   WTRGRILGRGSTATVY-AAAGHNSDEILAVKSSEVHRSEFLQREAKILSS-LSSPYVIGY 62

Query: 64  FGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQ-KKLSENTVKEYLKMILRGLSCIH 123
            G E  T+ +S     Y+L++EYA  GTL D   K   ++ E  V +Y + IL+GL  IH
Sbjct: 63  RGSE--TKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIH 122

Query: 124 KLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFKLKFRGTPMYMSPES 183
             G VH D+K +N++    I  K + KIADFG    C K+ +  F+    GTP +M+PE 
Sbjct: 123 SKGIVHCDVKGSNVV----ISEKGEAKIADFG----CAKRVDPVFESPVMGTPAFMAPEV 182

Query: 184 IYGE-VNTPLDIWSLGCILVEMVTGKHAW---DDCIDLEQLMLKLRVNMETPKIPEELSK 243
             GE      DIW++GC ++EMVTG   W   D   D   ++ ++  + ETP++P  L++
Sbjct: 183 ARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAE 242

Query: 244 EGKDFLEKCFDRNPKRRWTADMLLQHPYL 268
           E KDFLEKC  R    RWTA  LL HP+L
Sbjct: 243 EAKDFLEKCLKREANERWTATQLLNHPFL 259

[Close]

BLAST of MC09g_new0150 vs. ExPASy Swiss-Prot
Match: Q01389 (Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=BCK1 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.9e-36
Identity = 100/282 (35.46%), Postives = 152/282 (53.90%), Query Frame = 0


Query: 4    WVRVNDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSS-----------LLNEKRILK 63
            W++   +GKGS+G V+L    T   +  AVK  E+  YSS           L +E   LK
Sbjct: 1175 WMKGEMIGKGSFGAVYLCLNVTTG-EMMAVKQVEVPKYSSQNEAILSTVEALRSEVSTLK 1234

Query: 64   HFVGFPEIVQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLK 123
              +    IVQ  G E         +  YSL LEY AGG++  LIR   +  E  +K    
Sbjct: 1235 D-LDHLNIVQYLGFEN-------KNNIYSLFLEYVAGGSVGSLIRMYGRFDEPLIKHLTT 1294

Query: 124  MILRGLSCIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFKLKFR 183
             +L+GL+ +H  G +H D+K  N+L    +D     KI+DFG+SR+ +K    N  +  R
Sbjct: 1295 QVLKGLAYLHSKGILHRDMKADNLL----LDQDGICKISDFGISRK-SKDIYSNSDMTMR 1354

Query: 184  GTPMYMSPESIYGE--VNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETP 243
            GT  +M+PE +  +   +  +DIWSLGCI++EM  GK  W + +++   M K+  +   P
Sbjct: 1355 GTVFWMAPEMVDTKQGYSAKVDIWSLGCIVLEMFAGKRPWSN-LEVVAAMFKIGKSKSAP 1414

Query: 244  KIPEE----LSKEGKDFLEKCFDRNPKRRWTADMLLQHPYLE 269
             IPE+    +S+ G++FL+ CF+ NP++R TA+ LL HP+ E
Sbjct: 1415 PIPEDTLPLISQIGRNFLDACFEINPEKRPTANELLSHPFSE 1441

[Close]

BLAST of MC09g_new0150 vs. ExPASy Swiss-Prot
Match: F4HRJ4 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 8.2e-35
Identity = 88/275 (32.00%), Postives = 148/275 (53.82%), Query Frame = 0


Query: 2   TNWVRVNDLGKGSYGKVFLAKPTTRSLKYFAVKVAEL-------QHYSSLLNEKRILKHF 61
           + W +   LG G++G+V+L   + +  K  A+K  ++       +     LN++  L + 
Sbjct: 212 STWKKGKFLGSGTFGQVYLGFNSEKG-KMCAIKEVKVISDDQTSKECLKQLNQEINLLNQ 271

Query: 62  VGFPEIVQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMI 121
           +  P IVQ +G E+  E         S+ LEY +GG++  L++     +E  ++ Y + I
Sbjct: 272 LCHPNIVQYYGSELSEE-------TLSVYLEYVSGGSIHKLLKDYGSFTEPVIQNYTRQI 331

Query: 122 LRGLSCIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFKLKFRGT 181
           L GL+ +H    VH D+K ANIL    +D   ++K+ADFG+++        +  L F+G+
Sbjct: 332 LAGLAYLHGRNTVHRDIKGANIL----VDPNGEIKLADFGMAKHVT---AFSTMLSFKGS 391

Query: 182 PMYMSPESIYGE--VNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKI 241
           P +M+PE +  +      +DIWSLGC ++EM T K  W    +    + K+  + +TP+I
Sbjct: 392 PYWMAPEVVMSQNGYTHAVDIWSLGCTILEMATSKPPWSQ-FEGVAAIFKIGNSKDTPEI 451

Query: 242 PEELSKEGKDFLEKCFDRNPKRRWTADMLLQHPYL 268
           P+ LS + K+F+  C  RNP  R TA  LL+HP+L
Sbjct: 452 PDHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFL 470

[Close]

BLAST of MC09g_new0150 vs. NCBI nr
Match: XP_022150204.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 612 bits (1578), Expect = 3.48e-221
Identity = 297/298 (99.66%), Postives = 297/298 (99.66%), Query Frame = 0


Query: 1   MTNWVRVNDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEI 60
           MTNWVRV DLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEI
Sbjct: 1   MTNWVRVXDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEI 60

Query: 61  VQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSC 120
           VQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSC
Sbjct: 61  VQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSC 120

Query: 121 IHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFKLKFRGTPMYMSP 180
           IHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFKLKFRGTPMYMSP
Sbjct: 121 IHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFKLKFRGTPMYMSP 180

Query: 181 ESIYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEELSKEG 240
           ESIYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEELSKEG
Sbjct: 181 ESIYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEELSKEG 240

Query: 241 KDFLEKCFDRNPKRRWTADMLLQHPYLEQEKEAIKKDDDPTYSYLPHILYKFQSTIQI 298
           KDFLEKCFDRNPKRRWTADMLLQHPYLEQEKEAIKKDDDPTYSYLPHILYKFQSTIQI
Sbjct: 241 KDFLEKCFDRNPKRRWTADMLLQHPYLEQEKEAIKKDDDPTYSYLPHILYKFQSTIQI 298

[Close]

BLAST of MC09g_new0150 vs. NCBI nr
Match: XP_022154132.1 (mitogen-activated protein kinase kinase kinase YODA-like [Momordica charantia])

HSP 1 Score: 364 bits (934), Expect = 4.34e-123
Identity = 185/306 (60.46%), Postives = 231/306 (75.49%), Query Frame = 0


Query: 1   MTNWVRVNDLGKGSYGKVFLAKPTTRSLKYFAVKVAEL-----QHYSSLLNEKRILKHFV 60
           M NW+RV  LG+GSYG+VFLAKPT  SL YFA+K  +L       +SS L EK IL+ F+
Sbjct: 1   MANWIRVKPLGQGSYGEVFLAKPTADSLDYFAIKATQLPLHHCSSFSSPLREKCILECFI 60

Query: 61  GFPEIVQCFGGEMIT-EGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMI 120
           G PEIVQC+GGE+I  EGDSFD A + L++EYAAGG+LADLI+K+KKL+EN VK+YL+MI
Sbjct: 61  GCPEIVQCYGGEIIIMEGDSFDLASFFLIMEYAAGGSLADLIKKRKKLAENEVKDYLRMI 120

Query: 121 LRGLSCIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECN----FKLK 180
           LRG++CIH  GFVH DLKP NILAFPQ DGKMKLKI DFGL+RR  ++ E       + +
Sbjct: 121 LRGVACIHDKGFVHADLKPGNILAFPQPDGKMKLKITDFGLTRRSCEEEEAYDDYCVERR 180

Query: 181 FRGTPMYMSPESIYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETP 240
           FRGTP+YMSPES+YG+V  PLDIWS+GCI VE+V+GK  WD C D ++LMLKL   ME P
Sbjct: 181 FRGTPIYMSPESVYGQVKAPLDIWSVGCIAVEIVSGKPVWDGCPD-QKLMLKLVDKMEIP 240

Query: 241 KIPEELSKEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQEKEAIKKDDDPTYSYLPHILY 296
           KIPEE S+EGKDF+ +CFD  P+RRWTA+MLLQH YL++E +     D+    +L   + 
Sbjct: 241 KIPEEFSEEGKDFVGRCFDLEPERRWTAEMLLQHSYLKEENK-----DEIQMRFLHGHVN 300

[Close]

BLAST of MC09g_new0150 vs. NCBI nr
Match: XP_038896325.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 345 bits (884), Expect = 5.22e-115
Identity = 179/288 (62.15%), Postives = 213/288 (73.96%), Query Frame = 0


Query: 1   MTNWVRVNDLGKGSYGKVFLAKPTTRSLK----YFAVKVAELQHYSSLLNEKRILKHFVG 60
           M NWV V  +G+GSYG V LAK  T+       YFAVK+  LQ  SSLL E+++LKHF  
Sbjct: 1   MENWVLVKIIGRGSYGVVCLAKQMTKEKSDRHYYFAVKLTSLQRKSSLLWEEQVLKHFKD 60

Query: 61  FPEIVQCFGGEMITEGD-SFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMIL 120
            PEIVQC G E IT GD   D   Y+L LEYA GGTLADLI+++KKL E+ VK+YL+MIL
Sbjct: 61  CPEIVQCLGSE-ITFGDIPDDMVLYNLKLEYAPGGTLADLIKQRKKLPEDEVKDYLRMIL 120

Query: 121 RGLSCIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNK---KRECNFKLKFR 180
           +GLSCIH+ GFVHVDLKPAN+L FPQ DGKMKLKIADFGL+ RC      ++  +  KF+
Sbjct: 121 KGLSCIHRRGFVHVDLKPANVLVFPQSDGKMKLKIADFGLAERCKHIVDDQDRGYLRKFK 180

Query: 181 GTPMYMSPESIY-GEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPK 240
           GTP YMSPESI+  EVN   DIWSLGCILV+MV+GK  WDDC   EQLM +L  +   P 
Sbjct: 181 GTPKYMSPESIFFNEVNGTHDIWSLGCILVKMVSGKSVWDDCRGSEQLMTRLLNDKNIPT 240

Query: 241 IPEELSKEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQEKEAIKKDDD 279
           IP+ELSK+GKDF+++CF RN K+RWTADMLLQHPYL +E EA KK DD
Sbjct: 241 IPKELSKQGKDFIKRCFIRNYKQRWTADMLLQHPYLNEENEATKKGDD 287

[Close]

BLAST of MC09g_new0150 vs. NCBI nr
Match: XP_022152301.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 333 bits (853), Expect = 5.96e-111
Identity = 175/277 (63.18%), Postives = 201/277 (72.56%), Query Frame = 0


Query: 4   WVRVNDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEIVQC 63
           W RV  LG+GS GKVFLAKPT   + Y AVK A +   SSL+ EK IL+HFVG  EI+QC
Sbjct: 15  WTRVMLLGEGSQGKVFLAKPTMGPIYYVAVKAAFITKSSSLIIEKDILQHFVGCSEIIQC 74

Query: 64  FGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSCIHK 123
            G E+  E  S     Y+L+LEYAAGGTL +LI+KQK L EN VKEYLKMILRGLSCIH 
Sbjct: 75  IGDEITLEESSL---CYNLILEYAAGGTLENLIKKQKMLPENEVKEYLKMILRGLSCIHD 134

Query: 124 LGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRC-NKKREC-------NFKLKFRGTP 183
            GFVH DLKP NILAFPQ DGKMKLKIADFGLS+RC ++  E          K KF+GTP
Sbjct: 135 KGFVHGDLKPTNILAFPQSDGKMKLKIADFGLSKRCVHQDHEVVLDTVTPTSKPKFKGTP 194

Query: 184 MYMSPESI-YGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPK-IP 243
           +YMSPES+ +G ++ P DIWSLGCILVEM+TGK  W+ C    Q + KL   ME P  IP
Sbjct: 195 IYMSPESVAFGSISPPCDIWSLGCILVEMITGKEVWESCETSGQFIKKLVYEMELPADIP 254

Query: 244 EELSKEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQE 270
           EELS+EG+DFL  CFD +PKRRWTA MLLQHPYL QE
Sbjct: 255 EELSREGRDFLASCFDHDPKRRWTAHMLLQHPYLTQE 288

[Close]

BLAST of MC09g_new0150 vs. NCBI nr
Match: XP_031745125.1 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN44756.1 hypothetical protein Csa_016572 [Cucumis sativus])

HSP 1 Score: 324 bits (830), Expect = 7.56e-107
Identity = 177/299 (59.20%), Postives = 213/299 (71.24%), Query Frame = 0


Query: 1   MTNWVRVNDLGKGSYGKVFLAKPTTRSLK------YFAVKVAELQHYSSLLNEKRILKHF 60
           M NWV V  LG+GSY  V LAK + R         YFA+K+  LQH SSLL E+++LK F
Sbjct: 1   MENWVPVKVLGQGSYAVVCLAKQSIRKCSDNNLPYYFALKIYPLQHNSSLLWEEQVLKQF 60

Query: 61  VGFPEIVQCFGGEMITEGDSF--DSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLK 120
            G PEIVQ FG E IT G SF  D  FY+L LEYAAGGTL DLI+++ KL E+ VK+YL+
Sbjct: 61  KGCPEIVQYFGSE-ITRGGSFCNDKDFYTLKLEYAAGGTLDDLIKQRDKLPEDEVKDYLR 120

Query: 121 MILRGLSCIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFK---- 180
           MIL+GLSCIH  GFVHVDLKP NILAFPQ DGKMKLKIADFG + RC K R+ N +    
Sbjct: 121 MILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERC-KYRDDNGQHKRY 180

Query: 181 -----LKFRGTPMYMSPESI-YGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLK 240
                LKF+G+P YMSPESI + EV+   DIWSLGCILV+M++GK  WD   D +QLM++
Sbjct: 181 GYCSSLKFKGSPRYMSPESIIFNEVDDAHDIWSLGCILVKMISGKCVWDGYTDSKQLMIE 240

Query: 241 LRVNMETPKIPEELSKEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQEKEAIKKDDDPT 281
           +  N     IP ELS++GKDF+ KCF R+ K+RWTADMLLQHPYL QE EA  K+D+ T
Sbjct: 241 VLDNKIMATIPGELSEQGKDFIRKCFIRSYKQRWTADMLLQHPYLNQENEAPMKEDEAT 297

[Close]

BLAST of MC09g_new0150 vs. ExPASy TrEMBL
Match: A0A6J1D9A6 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111018431 PE=4 SV=1)

HSP 1 Score: 612 bits (1578), Expect = 1.68e-221
Identity = 297/298 (99.66%), Postives = 297/298 (99.66%), Query Frame = 0


Query: 1   MTNWVRVNDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEI 60
           MTNWVRV DLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEI
Sbjct: 1   MTNWVRVXDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEI 60

Query: 61  VQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSC 120
           VQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSC
Sbjct: 61  VQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSC 120

Query: 121 IHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFKLKFRGTPMYMSP 180
           IHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFKLKFRGTPMYMSP
Sbjct: 121 IHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFKLKFRGTPMYMSP 180

Query: 181 ESIYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEELSKEG 240
           ESIYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEELSKEG
Sbjct: 181 ESIYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEELSKEG 240

Query: 241 KDFLEKCFDRNPKRRWTADMLLQHPYLEQEKEAIKKDDDPTYSYLPHILYKFQSTIQI 298
           KDFLEKCFDRNPKRRWTADMLLQHPYLEQEKEAIKKDDDPTYSYLPHILYKFQSTIQI
Sbjct: 241 KDFLEKCFDRNPKRRWTADMLLQHPYLEQEKEAIKKDDDPTYSYLPHILYKFQSTIQI 298

[Close]

BLAST of MC09g_new0150 vs. ExPASy TrEMBL
Match: A0A6J1DKV0 (mitogen-activated protein kinase kinase kinase YODA-like OS=Momordica charantia OX=3673 GN=LOC111021457 PE=4 SV=1)

HSP 1 Score: 364 bits (934), Expect = 2.10e-123
Identity = 185/306 (60.46%), Postives = 231/306 (75.49%), Query Frame = 0


Query: 1   MTNWVRVNDLGKGSYGKVFLAKPTTRSLKYFAVKVAEL-----QHYSSLLNEKRILKHFV 60
           M NW+RV  LG+GSYG+VFLAKPT  SL YFA+K  +L       +SS L EK IL+ F+
Sbjct: 1   MANWIRVKPLGQGSYGEVFLAKPTADSLDYFAIKATQLPLHHCSSFSSPLREKCILECFI 60

Query: 61  GFPEIVQCFGGEMIT-EGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMI 120
           G PEIVQC+GGE+I  EGDSFD A + L++EYAAGG+LADLI+K+KKL+EN VK+YL+MI
Sbjct: 61  GCPEIVQCYGGEIIIMEGDSFDLASFFLIMEYAAGGSLADLIKKRKKLAENEVKDYLRMI 120

Query: 121 LRGLSCIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECN----FKLK 180
           LRG++CIH  GFVH DLKP NILAFPQ DGKMKLKI DFGL+RR  ++ E       + +
Sbjct: 121 LRGVACIHDKGFVHADLKPGNILAFPQPDGKMKLKITDFGLTRRSCEEEEAYDDYCVERR 180

Query: 181 FRGTPMYMSPESIYGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETP 240
           FRGTP+YMSPES+YG+V  PLDIWS+GCI VE+V+GK  WD C D ++LMLKL   ME P
Sbjct: 181 FRGTPIYMSPESVYGQVKAPLDIWSVGCIAVEIVSGKPVWDGCPD-QKLMLKLVDKMEIP 240

Query: 241 KIPEELSKEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQEKEAIKKDDDPTYSYLPHILY 296
           KIPEE S+EGKDF+ +CFD  P+RRWTA+MLLQH YL++E +     D+    +L   + 
Sbjct: 241 KIPEEFSEEGKDFVGRCFDLEPERRWTAEMLLQHSYLKEENK-----DEIQMRFLHGHVN 300

[Close]

BLAST of MC09g_new0150 vs. ExPASy TrEMBL
Match: A0A6J1DHA9 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111020049 PE=4 SV=1)

HSP 1 Score: 333 bits (853), Expect = 2.88e-111
Identity = 175/277 (63.18%), Postives = 201/277 (72.56%), Query Frame = 0


Query: 4   WVRVNDLGKGSYGKVFLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEIVQC 63
           W RV  LG+GS GKVFLAKPT   + Y AVK A +   SSL+ EK IL+HFVG  EI+QC
Sbjct: 15  WTRVMLLGEGSQGKVFLAKPTMGPIYYVAVKAAFITKSSSLIIEKDILQHFVGCSEIIQC 74

Query: 64  FGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLKMILRGLSCIHK 123
            G E+  E  S     Y+L+LEYAAGGTL +LI+KQK L EN VKEYLKMILRGLSCIH 
Sbjct: 75  IGDEITLEESSL---CYNLILEYAAGGTLENLIKKQKMLPENEVKEYLKMILRGLSCIHD 134

Query: 124 LGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRC-NKKREC-------NFKLKFRGTP 183
            GFVH DLKP NILAFPQ DGKMKLKIADFGLS+RC ++  E          K KF+GTP
Sbjct: 135 KGFVHGDLKPTNILAFPQSDGKMKLKIADFGLSKRCVHQDHEVVLDTVTPTSKPKFKGTP 194

Query: 184 MYMSPESI-YGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPK-IP 243
           +YMSPES+ +G ++ P DIWSLGCILVEM+TGK  W+ C    Q + KL   ME P  IP
Sbjct: 195 IYMSPESVAFGSISPPCDIWSLGCILVEMITGKEVWESCETSGQFIKKLVYEMELPADIP 254

Query: 244 EELSKEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQE 270
           EELS+EG+DFL  CFD +PKRRWTA MLLQHPYL QE
Sbjct: 255 EELSREGRDFLASCFDHDPKRRWTAHMLLQHPYLTQE 288

[Close]

BLAST of MC09g_new0150 vs. ExPASy TrEMBL
Match: A0A0A0KAC2 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378450 PE=4 SV=1)

HSP 1 Score: 324 bits (830), Expect = 3.66e-107
Identity = 177/299 (59.20%), Postives = 213/299 (71.24%), Query Frame = 0


Query: 1   MTNWVRVNDLGKGSYGKVFLAKPTTRSLK------YFAVKVAELQHYSSLLNEKRILKHF 60
           M NWV V  LG+GSY  V LAK + R         YFA+K+  LQH SSLL E+++LK F
Sbjct: 1   MENWVPVKVLGQGSYAVVCLAKQSIRKCSDNNLPYYFALKIYPLQHNSSLLWEEQVLKQF 60

Query: 61  VGFPEIVQCFGGEMITEGDSF--DSAFYSLVLEYAAGGTLADLIRKQKKLSENTVKEYLK 120
            G PEIVQ FG E IT G SF  D  FY+L LEYAAGGTL DLI+++ KL E+ VK+YL+
Sbjct: 61  KGCPEIVQYFGSE-ITRGGSFCNDKDFYTLKLEYAAGGTLDDLIKQRDKLPEDEVKDYLR 120

Query: 121 MILRGLSCIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFK---- 180
           MIL+GLSCIH  GFVHVDLKP NILAFPQ DGKMKLKIADFG + RC K R+ N +    
Sbjct: 121 MILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERC-KYRDDNGQHKRY 180

Query: 181 -----LKFRGTPMYMSPESI-YGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLK 240
                LKF+G+P YMSPESI + EV+   DIWSLGCILV+M++GK  WD   D +QLM++
Sbjct: 181 GYCSSLKFKGSPRYMSPESIIFNEVDDAHDIWSLGCILVKMISGKCVWDGYTDSKQLMIE 240

Query: 241 LRVNMETPKIPEELSKEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQEKEAIKKDDDPT 281
           +  N     IP ELS++GKDF+ KCF R+ K+RWTADMLLQHPYL QE EA  K+D+ T
Sbjct: 241 VLDNKIMATIPGELSEQGKDFIRKCFIRSYKQRWTADMLLQHPYLNQENEAPMKEDEAT 297

[Close]

BLAST of MC09g_new0150 vs. ExPASy TrEMBL
Match: A0A5D3DR57 (Mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold111G00640 PE=4 SV=1)

HSP 1 Score: 320 bits (821), Expect = 1.26e-105
Identity = 179/312 (57.37%), Postives = 213/312 (68.27%), Query Frame = 0


Query: 1   MTNWVRVNDLGKGSYGKVFLAKPTTRSLK--------YFAVKVAELQHYSSLLNEKRILK 60
           M NWV V  LG+GSYG V LAK ++   K        YFAVK++ LQH SSLL E+++LK
Sbjct: 1   MENWVPVKVLGQGSYGVVCLAKQSSAIRKKSDSNLPYYFAVKLSPLQHNSSLLWEEQVLK 60

Query: 61  HFVGFPEIVQCFGGEMITEG-----DSFDSAFYSLVLEYAAGGTLADLIRKQKKLSENTV 120
            F   PEIVQ  G E IT G     D  D  FY+L LEYAAGGTL DLI ++ KL E  V
Sbjct: 61  QFKDCPEIVQYLGSE-ITRGRDIRNDHVD--FYTLKLEYAAGGTLDDLIEQRDKLPEAEV 120

Query: 121 KEYLKMILRGLSCIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRE--- 180
           K+YL+MIL+GLSCIH  GFVHVDLKP NILAFPQ DGKMKLKIADFG + R   + +   
Sbjct: 121 KDYLRMILKGLSCIHSKGFVHVDLKPNNILAFPQSDGKMKLKIADFGQAERSKYRVDDQH 180

Query: 181 -----CNFKLKFRGTPMYMSPESI-YGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQ 240
                C    KF+GTP YMSPES+ + EVN   DIWSLGCILV+M++GK AWDDC D +Q
Sbjct: 181 KQYPPCCSSFKFKGTPRYMSPESVVFNEVNDADDIWSLGCILVKMISGKSAWDDCKDSKQ 240

Query: 241 LMLKLRVNMETPKIPEELSKEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQEKEA----- 281
           LM+KL  N   P IP ELS++GKDF++KCF RN K RWTA+MLLQHPYL +E EA     
Sbjct: 241 LMIKLLDNKIMPTIPGELSEQGKDFIKKCFIRNYKERWTAEMLLQHPYLNEENEAPMNNK 300

[Close]

BLAST of MC09g_new0150 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 204.5 bits (519), Expect = 1.2e-52
Identity = 118/286 (41.26%), Postives = 176/286 (61.54%), Query Frame = 0


Query: 4   WVRVNDLGKGSYGKVFLAKPTTRSLKYF----AVKVAELQHYSSLLNEKRILKHFVGFPE 63
           W+R   +G GS+  V LA  +  S K F    AVK + +   ++L NE+ +L       E
Sbjct: 3   WIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDCSE 62

Query: 64  IVQCFG-GEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQ-KKLSENTVKEYLKMILRG 123
           IV+CFG G  +  G+      Y+L LEYA+GG+LAD I+   + L E  V+ + + I++G
Sbjct: 63  IVRCFGEGRTVENGEE----IYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKG 122

Query: 124 LSCIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFKLKFRGTPMY 183
           L  IH  GF H D+K  N+L F    G   +KI+DFGL+    K+R     ++ RGTP+Y
Sbjct: 123 LCHIHGNGFTHCDIKLENVLVF----GDGDVKISDFGLA----KRRSGEVCVEIRGTPLY 182

Query: 184 MSPESI-YGEVNTPLDIWSLGCILVEMVTGKHAW--DDCI--DLEQLMLKLRVNMETPKI 243
           M+PES+ +GE  +P DIW+LGC +VEM +GK AW  +D +  ++  L++++    E P+I
Sbjct: 183 MAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRI 242

Query: 244 PEELSKEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQEKEAIKKDD 279
           P ELS+EGKDF+ KCF +N   RWTA+MLL HP+L  + E+ ++D+
Sbjct: 243 PVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDESGEEDE 276

[Close]

BLAST of MC09g_new0150 vs. TAIR 10
Match: AT2G41930.1 (Protein kinase superfamily protein )

HSP 1 Score: 202.2 bits (513), Expect = 5.8e-52
Identity = 117/277 (42.24%), Postives = 163/277 (58.84%), Query Frame = 0


Query: 7   VNDLGKGSYGKV--FLAKPTTRSLKYFAVKVAELQHYSSLLNEKRILKHFVGFPEIVQCF 66
           V  LGKG+YG V  F  K    SL Y AVK+ + ++Y S+  E RIL    G P IVQ  
Sbjct: 8   VKVLGKGTYGSVELFSHKQNDGSLLYNAVKIMDSENYGSIDQEFRILSELRGCPCIVQLC 67

Query: 67  GGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRKQK-KLSENTVKEYLKMILRGLSCIHK 126
           G  ++   D      Y + +EYAA GTL + I++ + KLS++ +K++ +MIL+GL  IH 
Sbjct: 68  GNSLVQGIDCNGKKVYMMSMEYAAAGTLTNFIKRNRTKLSDSVIKDFTRMILQGLVSIHN 127

Query: 127 LGFVHVDLKPANILAFPQIDG-----KMKLKIADFGLSRRCNKKREC-NFKLKFRGTPMY 186
            G+VH DLKP NIL FP  D        +LKI+DFG+S R   K  C      + GT +Y
Sbjct: 128 HGYVHCDLKPDNILLFPLYDKDTWNCSYELKISDFGISTRAGDKSGCWRVDEPWVGTSIY 187

Query: 187 MSPESIYG--EVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEE 246
           MSPES+     V   LD+WSLGCI+++M TGK  W   +  E+ +  L +N + P+IPE 
Sbjct: 188 MSPESVSDGTTVEKTLDLWSLGCIVLKMYTGKRPW---LGFEKDVKSLLLNQKAPEIPET 247

Query: 247 LSKEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQEKE 273
           L  + + FLEKCF R P+ R +A  LL HP+L  +++
Sbjct: 248 LPCDARLFLEKCFSRKPEERGSASELLLHPFLTGDEK 281

[Close]

BLAST of MC09g_new0150 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 201.8 bits (512), Expect = 7.6e-52
Identity = 115/288 (39.93%), Postives = 173/288 (60.07%), Query Frame = 0


Query: 4   WVRVNDLGKGSYGKVFLAKPTTRSLKYF----AVKVAELQHYSSLLNEKRILKHF-VGFP 63
           W+R   +G G++  V LA  +      F    AVK A+    +SL NEK +L +      
Sbjct: 3   WIRGETIGYGTFSTVSLATRSNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDDCN 62

Query: 64  EIVQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRK--QKKLSENTVKEYLKMILR 123
           EIV+CFG +   E        ++L LEYA+ G+L   ++K   + + E+TV+ +   +LR
Sbjct: 63  EIVRCFGEDRTVEN---GEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLR 122

Query: 124 GLSCIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFKLKFRGTPM 183
           GL  IH  GF H DLK  NIL F    G   +KIADFGL++R       N+ ++ RGTP+
Sbjct: 123 GLRHIHANGFAHCDLKLGNILLF----GDGAVKIADFGLAKRIGDLTALNYGVQIRGTPL 182

Query: 184 YMSPESIY-GEVNTPLDIWSLGCILVEMVTGKHAWD--DCIDLEQLMLKLRVNMETPKIP 243
           YM+PES+   E  +  D+W+LGC++VEM +GK AW   +  +   L+L++ V  E P IP
Sbjct: 183 YMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIP 242

Query: 244 EELSKEGKDFLEKCFDRNPKRRWTADMLLQHPY--LEQEKEAIKKDDD 280
           EELS++G+DFL KCF ++PK+RWTA+MLL HP+  ++ + + + K++D
Sbjct: 243 EELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEED 283

[Close]

BLAST of MC09g_new0150 vs. TAIR 10
Match: AT2G42550.1 (Protein kinase superfamily protein )

HSP 1 Score: 198.0 bits (502), Expect = 1.1e-50
Identity = 118/273 (43.22%), Postives = 159/273 (58.24%), Query Frame = 0


Query: 7   VNDLGKGSYGKVFLAKPTTRSLK----YFAVKVAELQHYSSLLNEKRILKHFVGFPEIVQ 66
           V  L KG+YG V L K   R       Y AVK AE + Y+SL  E +IL    G   IVQ
Sbjct: 8   VKFLRKGAYGSVDLVKYIKRDDNALPLYAAVKTAECEDYNSLEREIQILSKLEGCRRIVQ 67

Query: 67  CFGGEMITEG-DSFDSAFYSLVLEYAAGGTLADLI--RKQKKLSENTVKEYLKMILRGLS 126
           C+G   + E  D      Y +V+EYAA G+L   +   K +KL E  +K++ +MIL+GL 
Sbjct: 68  CYGNYTLEEDFDVGGFRVYKMVMEYAAAGSLFSFMDSYKDRKLPETMIKDFTRMILQGLV 127

Query: 127 CIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKREC-NFKLKFRGTPMYM 186
            +H+LG+VH DLKP N+L FP      +LKI+DFG SR+  +  +C +  L F GTP+YM
Sbjct: 128 SVHRLGYVHCDLKPDNLLVFP-CRQSYELKISDFGSSRKVGEYSDCWDVDLPFVGTPVYM 187

Query: 187 SPESI-YGEVNTPLDIWSLGCILVEMVTGKHAWDDCIDLEQLMLKLRVNMETPKIPEELS 246
           SPES+  G     LD+WSLGCI++EM TG   W + ++ E L   L    + P+IP+ L 
Sbjct: 188 SPESVRSGVAEKALDLWSLGCIVLEMYTGVIPWSE-VEFEDLAPALSKG-KAPEIPKSLP 247

Query: 247 KEGKDFLEKCFDRNPKRRWTADMLLQHPYLEQE 271
            + + FLE CF RNPK R +A  LL H +L  E
Sbjct: 248 CDARKFLETCFSRNPKERGSASDLLSHQFLRGE 277

[Close]

BLAST of MC09g_new0150 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 191.8 bits (486), Expect = 7.8e-49
Identity = 117/305 (38.36%), Postives = 172/305 (56.39%), Query Frame = 0


Query: 4   WVRVNDLGKGSYGKVFLAKPTTRSLKY---FAVKVAELQHYSSLLNEKRILKHFVGFPEI 63
           WVR   +G G++  V  A  +  S  +    AVK  +    +SL NEK +L      PEI
Sbjct: 3   WVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEI 62

Query: 64  VQCFGGEMITEGDSFDSAFYSLVLEYAAGGTLADLIRK--QKKLSENTVKEYLKMILRGL 123
           ++C+G +   E        ++L+LEYA+ G+LA  ++K   + L E+TV+ +   +LRGL
Sbjct: 63  IRCYGEDSTVEN---GEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGL 122

Query: 124 SCIHKLGFVHVDLKPANILAFPQIDGKMKLKIADFGLSRRCNKKRECNFK-LKFRGTPMY 183
             IH  GF H D+K ANIL F   DG   +KIADFGL+ R +       K ++ RGTP+Y
Sbjct: 123 RHIHAKGFAHCDIKLANILLFN--DG--SVKIADFGLAMRVDGDLTALRKSVEIRGTPLY 182

Query: 184 MSPESIY-GEVNTPLDIWSLGCILVEMVTGKHAWD--DCIDLEQLMLKLRVNMETPKIPE 243
           M+PE +   E  +  D+W+LGC +VEM +GK AW   +      L++++ V  E PKIPE
Sbjct: 183 MAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPE 242

Query: 244 ELSKEGKDFLEKCFDRNPKRRWTADMLLQHPYL-------EQEKEAIKKDDDPTYSYLPH 293
            LS+EGKDFL KCF ++P +RWTA+MLL H ++        +E   +K  D+      P 
Sbjct: 243 MLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPK 300

The following BLAST results are available for this feature:

BLAST of MC09g_new0150 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	1.1e-47	38.36	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
Q54R82	4.2e-39	37.04	Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=...	[more]
O80888	2.7e-38	37.92	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]
Q01389	1.9e-36	35.46	Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS=Saccharomyces cerevisiae (str...	[more]
F4HRJ4	8.2e-35	32.00	Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of MC09g_new0150 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150204.1	3.48e-221	99.66	mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia]	[more]
XP_022154132.1	4.34e-123	60.46	mitogen-activated protein kinase kinase kinase YODA-like [Momordica charantia]	[more]
XP_038896325.1	5.22e-115	62.15	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]
XP_022152301.1	5.96e-111	63.18	mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia]	[more]
XP_031745125.1	7.56e-107	59.20	mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN44756.1 ...	[more]

BLAST of MC09g_new0150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D9A6	1.68e-221	99.66	mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX...	[more]
A0A6J1DKV0	2.10e-123	60.46	mitogen-activated protein kinase kinase kinase YODA-like OS=Momordica charantia ...	[more]
A0A6J1DHA9	2.88e-111	63.18	mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX...	[more]
A0A0A0KAC2	3.66e-107	59.20	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378...	[more]
A0A5D3DR57	1.26e-105	57.37	Mitogen-activated protein kinase kinase kinase ANP1-like OS=Cucumis melo var. ma...	[more]

BLAST of MC09g_new0150 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36950.1	1.2e-52	41.26	mitogen-activated protein kinase kinase kinase 21	[more]
AT2G41930.1	5.8e-52	42.24	Protein kinase superfamily protein	[more]
AT5G67080.1	7.6e-52	39.93	mitogen-activated protein kinase kinase kinase 19	[more]
AT2G42550.1	1.1e-50	43.22	Protein kinase superfamily protein	[more]
AT3G50310.1	7.8e-49	38.36	mitogen-activated protein kinase kinase kinase 20	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 4..267 e-value: 7.5E-72 score: 254.7
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 6..267 e-value: 6.9E-58 score: 196.1
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 4..267 score: 42.534164
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 2..295 e-value: 5.8E-69 score: 234.8
None	No IPR available	PIRSR	PIRSR000620-2	PIRSR000620-2	coord: 8..262 e-value: 3.4E-12 score: 43.8
None	No IPR available	PANTHER	PTHR48011:SF51	PROTEIN KINASE SUPERFAMILY PROTEIN	coord: 1..275
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 1..275
None	No IPR available	CDD	cd06606	STKc_MAPKKK	coord: 3..267 e-value: 4.40471E-101 score: 294.813
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 10..34
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 3..289

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g_new0150.1	MC09g_new0150.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0004672	protein kinase activity