MC09g_new0135 (gene) Bitter gourd (Dali-11) v1

BLAST of MC09g_new0135 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 3.1e-44
Identity = 117/353 (33.14%), Postives = 190/353 (53.82%), Query Frame = 0

Query: 15  IRLCLSMNVASLVANETTAIDLMKALENRYEKPSANNKVYLVRRLFNIQMEENTSVNSHI 74
           IRL LS +V + + +E TA  +   LE+ Y   +  NK+YL ++L+ + M E T+  SH+
Sbjct: 63  IRLHLSDDVVNNIIDEDTARGIWTRLESLYMSKTLTNKLYLKKQLYALHMSEGTNFLSHL 122

Query: 75  NKVTKLINQLASVKITFSDEVNAIQLLTSLPESWETMKTAVSNSLGDKSLKFSEICDAAI 134
           N    LI QLA++ +   +E  AI LL SLP S++ + T + +  G  +++  ++  A +
Sbjct: 123 NVFNGLITQLANLGVKIEEEDKAILLLNSLPSSYDNLATTILH--GKTTIELKDVTSALL 182

Query: 135 AEEIRRKGSGKESTSV--QGSALAVEKGREKVASDDRQQKRSRRNWKRDAECFHCHKKGH 194
             E  RK    +  ++  +G   + ++          + K   R+  R   C++C++ GH
Sbjct: 183 LNEKMRKKPENQGQALITEGRGRSYQRSSNNYGRSGARGKSKNRSKSRVRNCYNCNQPGH 242

Query: 195 IKKNC---RILKEDLKRYMTESNAVV-----DDALVCVDSNTE---IGNQSSEWVIDSAA 254
            K++C   R  K +      + N        D+ ++ ++   E   +    SEWV+D+AA
Sbjct: 243 FKRDCPNPRKGKGETSGQKNDDNTAAMVQNNDNVVLFINEEEECMHLSGPESEWVVDTAA 302

Query: 255 SVHISSNRRLFTSFRRGNRGHVRMGNGRLSKTKGIGNIRLKTNSGTELLLRDVRYVPSIK 314
           S H +  R LF  +  G+ G V+MGN   SK  GIG+I +KTN G  L+L+DVR+VP ++
Sbjct: 303 SHHATPVRDLFCRYVAGDFGTVKMGNTSYSKIAGIGDICIKTNVGCTLVLKDVRHVPDLR 362

Query: 315 MNLISTGKLDDDVYQSEFGGNQWKLTKGSKLVAIGHRRSTVYTSQLSVASGSL 355
           MNLIS   LD D Y+S F   +W+LTKGS ++A G  R T+Y +   +  G L
Sbjct: 363 MNLISGIALDRDGYESYFANQKWRLTKGSLVIAKGVARGTLYRTNAEICQGEL 413

BLAST of MC09g_new0135 vs. ExPASy Swiss-Prot
Match: P25601 (Putative transposon Ty5-1 protein YCL075W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY5B PE=5 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 3.7e-05
Identity = 30/87 (34.48%), Postives = 47/87 (54.02%), Query Frame = 0

Query: 220 VCVDSNTEIGNQSSEWVIDSAASVHISSNRRLFTSFRRGNRGHVRMGNGRLSKTKGIGNI 279
           +C+ S+T    +SSEW+ D+  + H+  +R +F+SF R +R     G G      G G +
Sbjct: 64  LCMLSSTVPATKSSEWIFDTGCTSHMCHDRSIFSSFTRSSRKDFVRGVGGSIPIMGSGTV 123

Query: 280 RLKTNSGTELLLRDVRYVPSIKMNLIS 307
            + T     + L DV YVP + +NLIS
Sbjct: 124 NIGT-----VQLHDVSYVPDLPVNLIS 145

BLAST of MC09g_new0135 vs. NCBI nr
Match: KAF7115099.1 (hypothetical protein RHSIM_RhsimUnG0064500 [Rhododendron simsii])

HSP 1 Score: 261 bits (667), Expect = 3.52e-77
Identity = 153/372 (41.13%), Postives = 224/372 (60.22%), Query Frame = 0

Query: 5   WRRMNRQLIN-IRLCLSMNVASLVANETTAIDLMKALENRYEKPSANNKVYLVRRLFNIQ 64
           W+  NR+ +  IR  +  +V   VA ET A +L   LE  YE+ +A NK  L+RRL N++
Sbjct: 51  WKVTNRKAVALIRTWVDQSVFHHVAQETNAYELWTKLEAMYERKTAQNKASLIRRLVNLK 110

Query: 65  MEENTSVNSHINKVTKLINQLASVKITFSDEVNAIQLLTSLPESWETMKTAVSNSLGDKS 124
            ++  SV  H++    LINQL+++K+   +E+ A+ LL+SLP+SWET+   +SNS     
Sbjct: 111 YKDGRSVAEHMSDFQGLINQLSTMKLVLDEELQALLLLSSLPDSWETLVVTISNSAPSGM 170

Query: 125 LKFSEICDAAIAEEIRRKGSGKESTSVQGSALAVEK---------GREKVASDDRQQKRS 184
           +    + D    EE RRK  G   TSV   AL V+          G ++  S DR + +S
Sbjct: 171 VTMDMVKDGMFNEEARRKEQG---TSVDTEALVVQNRGRSRTRNSGGDRNKSKDRSKSKS 230

Query: 185 RRNWK--RDAECFHCHKKGHIKKNCRILKEDLKRYM-------TESNAVVDDA---LVCV 244
           R   K   + ECFHCHK GH++K CRIL+++LK+          ++ AV  D    +VC 
Sbjct: 231 RGTSKPRSEIECFHCHKMGHVRKECRILQKELKKGKGAEISGKKDTTAVASDGEVIIVCD 290

Query: 245 DSNTEIGNQSSEWVIDSAASVHISSNRRLFTSFRRGNRGHVRMGNGRLSKTKGIGNIRLK 304
           D    +  Q   WVIDS AS H++S R  F S+  G+ GHVRMGN  +SK  G+G++ L+
Sbjct: 291 DGFVGLTGQDLSWVIDSGASFHVTSQRDFFASYIHGDFGHVRMGNEGVSKIVGMGDVCLE 350

Query: 305 TNSGTELLLRDVRYVPSIKMNLISTGKLDDDVYQSEFGGNQWKLTKGSKLVAIGHRRSTV 354
           TN+G +LLL+DVR+VP I++NLIS GKLDD+ Y+++FG  +WKL+KGS +VA G + ST+
Sbjct: 351 TNTGCKLLLKDVRHVPDIRLNLISAGKLDDEGYKNQFGDGKWKLSKGSLVVARGKKSSTL 410

BLAST of MC09g_new0135 vs. NCBI nr
Match: KAF7129225.1 (hypothetical protein RHSIM_Rhsim10G0050800 [Rhododendron simsii])

HSP 1 Score: 262 bits (670), Expect = 5.05e-77
Identity = 154/372 (41.40%), Postives = 227/372 (61.02%), Query Frame = 0

Query: 5   WRRMNRQLIN-IRLCLSMNVASLVANETTAIDLMKALENRYEKPSANNKVYLVRRLFNIQ 64
           W+  NR+ +  IR  +  +V   VA ET A +L   LE  YE+ +A NK  L+RRL N++
Sbjct: 51  WKVTNRKAVALIRTWVDQSVFHHVAQETNAYELWTKLEAMYERKTAQNKASLIRRLVNLK 110

Query: 65  MEENTSVNSHINKVTKLINQLASVKITFSDEVNAIQLLTSLPESWETMKTAVSNSLGDKS 124
            ++  SV  H++    LINQL+++K+   +E+ A+ LL+SLP+SWET+   +SNS     
Sbjct: 111 YKDGRSVAEHMSDFQGLINQLSTMKLVLDEELQALLLLSSLPDSWETLVVTISNSAPSGM 170

Query: 125 LKFSEICDAAIAEEIRRKGSGKESTSVQGSALAVE-KGREKVA--------SDDRQQKRS 184
           +    + D    EE RRK  G   TSV   AL V+ +GR ++         S DR + +S
Sbjct: 171 VTMDMVKDGMFNEEARRKEQG---TSVDTEALVVQNRGRSRIRNSGGDRNKSKDRSRSKS 230

Query: 185 RRNWK--RDAECFHCHKKGHIKKNCRILKEDLKRYM-------TESNAVVDDA---LVCV 244
           R   K   + ECFHCHK GH++K CRIL+++LK+          ++ AV  D    +VC 
Sbjct: 231 RGTSKPRSEIECFHCHKMGHMRKECRILQKELKKGKGAEISGKKDTTAVASDGEVIIVCD 290

Query: 245 DSNTEIGNQSSEWVIDSAASVHISSNRRLFTSFRRGNRGHVRMGNGRLSKTKGIGNIRLK 304
           D    +  Q   WVIDS AS H++S R  F S+  G+ GHVRMGN  +SK  G+G++ L+
Sbjct: 291 DGFVGLTGQDLSWVIDSGASFHVTSQRDFFASYTHGDFGHVRMGNEGVSKIVGMGDVCLE 350

Query: 305 TNSGTELLLRDVRYVPSIKMNLISTGKLDDDVYQSEFGGNQWKLTKGSKLVAIGHRRSTV 354
           TN+G +LLL+DVR+VP+I++NLIS GKLDD+ Y+++FG  +WKL+KGS +VA G + ST+
Sbjct: 351 TNTGCKLLLKDVRHVPNIRLNLISAGKLDDEGYKNQFGDGKWKLSKGSLVVARGKKSSTL 410

BLAST of MC09g_new0135 vs. NCBI nr
Match: KAF7129546.1 (hypothetical protein RHSIM_Rhsim10G0154200 [Rhododendron simsii])

HSP 1 Score: 261 bits (666), Expect = 4.05e-76
Identity = 152/372 (40.86%), Postives = 226/372 (60.75%), Query Frame = 0

Query: 5   WRRMNRQLIN-IRLCLSMNVASLVANETTAIDLMKALENRYEKPSANNKVYLVRRLFNIQ 64
           W+  NR+ +  IR  +  +V   VA ET A +L   LE  YE+ +A NK  L+RRL N++
Sbjct: 51  WKVTNRKAVALIRTWVDQSVFHHVAQETNAYELWTKLEAMYERKTAQNKASLIRRLVNLK 110

Query: 65  MEENTSVNSHINKVTKLINQLASVKITFSDEVNAIQLLTSLPESWETMKTAVSNSLGDKS 124
            ++  SV  H++    LINQL+++K+   +E+ A+ LL+SLP+SWET+   +SNS     
Sbjct: 111 YKDGRSVAEHMSDFQGLINQLSTMKLVLDEELQALLLLSSLPDSWETLVVTISNSAPSGM 170

Query: 125 LKFSEICDAAIAEEIRRKGSGKESTSVQGSALAVE-KGREKVASD--DRQQKRSRRNWKR 184
           +    + D    EE RRK  G   TSV   AL V+ +GR ++ +   DR + + R   K 
Sbjct: 171 VTMDMVKDGMFNEEARRKEQG---TSVDTEALVVQNRGRSRIRNSGGDRNKFKDRSKSKS 230

Query: 185 --------DAECFHCHKKGHIKKNCRILKEDLKRYM-------TESNAVVDDA---LVCV 244
                   + ECFHCHK GH++K CRIL+++LK+          ++ AV  D    +VC 
Sbjct: 231 TGTSKPRSEIECFHCHKMGHVRKECRILQKELKKGKGAEISGKKDTTAVASDGEVIIVCD 290

Query: 245 DSNTEIGNQSSEWVIDSAASVHISSNRRLFTSFRRGNRGHVRMGNGRLSKTKGIGNIRLK 304
           D    +  Q   WVIDS AS H++S R  F S+  G+ GHVRMGN  +SK  G+G++ L+
Sbjct: 291 DGFVGLTGQDLSWVIDSGASFHVTSQRDFFASYTHGDFGHVRMGNEGVSKIVGMGDVCLE 350

Query: 305 TNSGTELLLRDVRYVPSIKMNLISTGKLDDDVYQSEFGGNQWKLTKGSKLVAIGHRRSTV 354
           TN+G +LLL+DVR+VP I++NLIS GKLDD+ Y+++FG  +WKL+KGS +VA G + ST+
Sbjct: 351 TNTGCKLLLKDVRHVPDIRLNLISAGKLDDEGYKNQFGDGKWKLSKGSLIVARGKKSSTL 410

BLAST of MC09g_new0135 vs. NCBI nr
Match: KAF7121453.1 (hypothetical protein RHSIM_Rhsim13G0116100 [Rhododendron simsii])

HSP 1 Score: 261 bits (668), Expect = 5.33e-76
Identity = 153/372 (41.13%), Postives = 224/372 (60.22%), Query Frame = 0

Query: 5   WRRMNRQLIN-IRLCLSMNVASLVANETTAIDLMKALENRYEKPSANNKVYLVRRLFNIQ 64
           W+  NR+ +  IR  +  +V   VA ET A +L   LE  YE+ +A NK  L+RRL N++
Sbjct: 29  WKVTNRKAVALIRTWVDQSVFHHVAQETNAYELWTKLEAMYERKTAQNKASLIRRLVNLK 88

Query: 65  MEENTSVNSHINKVTKLINQLASVKITFSDEVNAIQLLTSLPESWETMKTAVSNSLGDKS 124
            ++  SV  H++    LINQL+++K+   +E+ A+ LL+SLP+SWET+   +SNS     
Sbjct: 89  YKDGRSVAEHMSDFQGLINQLSTMKLVLDEELQALLLLSSLPDSWETLVVTISNSAPSGM 148

Query: 125 LKFSEICDAAIAEEIRRKGSGKESTSVQGSALAVEK---------GREKVASDDRQQKRS 184
           +    + D    EE RRK  G   TSV   AL V+          G ++  S DR + +S
Sbjct: 149 VTMDMVKDGMFNEEARRKEQG---TSVDTEALVVQNRGRSRTRNSGGDRNKSKDRSRSKS 208

Query: 185 RRNWK--RDAECFHCHKKGHIKKNCRILKEDLKRYMT-------ESNAVVDDA---LVCV 244
           R   K   + ECFHCHK GH++K CRIL+++LK+          ++ AV  D    +VC 
Sbjct: 209 RGTSKPRSEIECFHCHKMGHMRKECRILQKELKKGKVVESSGKKDTTAVASDGEVIIVCD 268

Query: 245 DSNTEIGNQSSEWVIDSAASVHISSNRRLFTSFRRGNRGHVRMGNGRLSKTKGIGNIRLK 304
           D    +  Q   WVIDS AS H++S R  F S+  G+ GHVRMGN  +SK  G+G++ L+
Sbjct: 269 DGFVGLTGQDLSWVIDSGASFHVTSQRDFFASYTHGDFGHVRMGNEGVSKIVGMGDVCLE 328

Query: 305 TNSGTELLLRDVRYVPSIKMNLISTGKLDDDVYQSEFGGNQWKLTKGSKLVAIGHRRSTV 354
           TN+G +LLL+DVR+VP I++NLIS GKLDD+ Y+++FG  +WKL+KGS +VA G + ST+
Sbjct: 329 TNTGCKLLLKDVRHVPDIRLNLISAGKLDDEGYKNQFGDGKWKLSKGSLVVARGKKSSTL 388

BLAST of MC09g_new0135 vs. NCBI nr
Match: RVW14266.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 263 bits (673), Expect = 6.61e-75
Identity = 151/361 (41.83%), Postives = 232/361 (64.27%), Query Frame = 0

Query: 5   WRRMNRQLIN-IRLCLSMNVASLVANETTAIDLMKALENRYEKPSANNKVYLVRRLFNIQ 64
           W++++R+ +  IR  +  +V   V+ E +A  L   LE+ Y++ +A NK +L R+L N +
Sbjct: 265 WKKLDRKAVGFIRQWIDDSVFHHVSTENSAHGLWTKLESLYDRKTAVNKAFLFRKLVNSK 324

Query: 65  MEENTSVNSHINKVTKLINQLASVKITFSDEVNAIQLLTSLPESWETMKTAVSNSLGDKS 124
            +E T +  H+N++  ++NQLA++KITF DE+ A+ LL+SLPESWET+   VSNS  D  
Sbjct: 325 YKEGTPIAEHLNEIKSIVNQLAAMKITFDDELQALLLLSSLPESWETLVVTVSNSTPDGV 384

Query: 125 LKFSEICDAAIAEEIRRKGSGKESTSVQGSALAVE---KGREKV-ASDDRQQKRSRRNW- 184
           +  S++  + + EE RRK SG    S    AL +E   +GR K  AS +R + R R +  
Sbjct: 385 VTMSQVTSSLLNEETRRKSSG----SSHSEALVIENRGRGRSKSKASHNRDKSRGRSSSI 444

Query: 185 -KRDAECFHCHKKGHIKKNCRILK-------EDLKRYMTESNAVVDDALVCV--DSNTEI 244
            K+D EC++CHKKGH+K+ CR LK       ++ ++   ++  V D  L+ +  D +  +
Sbjct: 445 SKKDVECYYCHKKGHMKRECRKLKFKEQNKEKNSEKKQNDTAVVTDGELMIIRDDVSVNL 504

Query: 245 GNQSSEWVIDSAASVHISSNRRLFTSFRRGNRGHVRMGNGRLSKTKGIGNIRLKTNSGTE 304
             Q ++WVIDS AS H++S    FTS+ +G+ G+VRMGN  +SK  G+G+I L+TN+G +
Sbjct: 505 IGQETDWVIDSGASFHVTSRADFFTSYSQGDFGNVRMGNEDVSKIVGMGDICLETNTGCK 564

Query: 305 LLLRDVRYVPSIKMNLISTGKLDDDVYQSEFGGNQWKLTKGSKLVAIGHRRSTVYTSQLS 349
           LLLRDVR+VP I++NLISTGKLDD+ Y + F   +WKL+KGS +VA G +  ++YT Q  
Sbjct: 565 LLLRDVRHVPDIRLNLISTGKLDDEGYNNNFSDGKWKLSKGSLVVAKGKKTCSLYTVQAK 621

BLAST of MC09g_new0135 vs. ExPASy TrEMBL
Match: A0A438BTH6 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_4171 PE=4 SV=1)

HSP 1 Score: 263 bits (673), Expect = 3.20e-75
Identity = 151/361 (41.83%), Postives = 232/361 (64.27%), Query Frame = 0

Query: 5   WRRMNRQLIN-IRLCLSMNVASLVANETTAIDLMKALENRYEKPSANNKVYLVRRLFNIQ 64
           W++++R+ +  IR  +  +V   V+ E +A  L   LE+ Y++ +A NK +L R+L N +
Sbjct: 265 WKKLDRKAVGFIRQWIDDSVFHHVSTENSAHGLWTKLESLYDRKTAVNKAFLFRKLVNSK 324

Query: 65  MEENTSVNSHINKVTKLINQLASVKITFSDEVNAIQLLTSLPESWETMKTAVSNSLGDKS 124
            +E T +  H+N++  ++NQLA++KITF DE+ A+ LL+SLPESWET+   VSNS  D  
Sbjct: 325 YKEGTPIAEHLNEIKSIVNQLAAMKITFDDELQALLLLSSLPESWETLVVTVSNSTPDGV 384

Query: 125 LKFSEICDAAIAEEIRRKGSGKESTSVQGSALAVE---KGREKV-ASDDRQQKRSRRNW- 184
           +  S++  + + EE RRK SG    S    AL +E   +GR K  AS +R + R R +  
Sbjct: 385 VTMSQVTSSLLNEETRRKSSG----SSHSEALVIENRGRGRSKSKASHNRDKSRGRSSSI 444

Query: 185 -KRDAECFHCHKKGHIKKNCRILK-------EDLKRYMTESNAVVDDALVCV--DSNTEI 244
            K+D EC++CHKKGH+K+ CR LK       ++ ++   ++  V D  L+ +  D +  +
Sbjct: 445 SKKDVECYYCHKKGHMKRECRKLKFKEQNKEKNSEKKQNDTAVVTDGELMIIRDDVSVNL 504

Query: 245 GNQSSEWVIDSAASVHISSNRRLFTSFRRGNRGHVRMGNGRLSKTKGIGNIRLKTNSGTE 304
             Q ++WVIDS AS H++S    FTS+ +G+ G+VRMGN  +SK  G+G+I L+TN+G +
Sbjct: 505 IGQETDWVIDSGASFHVTSRADFFTSYSQGDFGNVRMGNEDVSKIVGMGDICLETNTGCK 564

Query: 305 LLLRDVRYVPSIKMNLISTGKLDDDVYQSEFGGNQWKLTKGSKLVAIGHRRSTVYTSQLS 349
           LLLRDVR+VP I++NLISTGKLDD+ Y + F   +WKL+KGS +VA G +  ++YT Q  
Sbjct: 565 LLLRDVRHVPDIRLNLISTGKLDDEGYNNNFSDGKWKLSKGSLVVAKGKKTCSLYTVQAK 621

BLAST of MC09g_new0135 vs. ExPASy TrEMBL
Match: A0A438HI91 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_1199 PE=4 SV=1)

HSP 1 Score: 263 bits (673), Expect = 3.89e-74
Identity = 151/361 (41.83%), Postives = 232/361 (64.27%), Query Frame = 0

Query: 5   WRRMNRQLIN-IRLCLSMNVASLVANETTAIDLMKALENRYEKPSANNKVYLVRRLFNIQ 64
           W++++R+ +  IR  +  +V   V+ E +A  L   LE+ Y++ +A NK +L R+L N +
Sbjct: 29  WKKLDRKAVGFIRQWIGDSVFHHVSTENSAHGLWTKLESLYDRKTAVNKAFLFRKLVNSK 88

Query: 65  MEENTSVNSHINKVTKLINQLASVKITFSDEVNAIQLLTSLPESWETMKTAVSNSLGDKS 124
            +E T +  H+N++  ++NQLA++KITF DE+ A+ LL+SLPESWET+   VSNS  D  
Sbjct: 89  YKEGTPIAEHLNEIKSIVNQLAAMKITFDDELQALLLLSSLPESWETLVVTVSNSTPDGV 148

Query: 125 LKFSEICDAAIAEEIRRKGSGKESTSVQGSALAVE---KGREKV-ASDDRQQKRSRRNW- 184
           +  S++  + + EE RRK SG    S    AL +E   +GR K  AS +R + R R +  
Sbjct: 149 VTMSQVTSSLLNEETRRKSSG----SSHSEALVIENRGRGRSKSKASHNRDKSRGRSSSI 208

Query: 185 -KRDAECFHCHKKGHIKKNCRILK-------EDLKRYMTESNAVVDDALVCV--DSNTEI 244
            K+D EC++CHKKGH+K+ CR LK       ++ ++   ++  V D  L+ +  D +  +
Sbjct: 209 SKKDVECYYCHKKGHMKRECRKLKFKEQNKEKNSEKKQNDTAVVTDGELMIIRDDVSVNL 268

Query: 245 GNQSSEWVIDSAASVHISSNRRLFTSFRRGNRGHVRMGNGRLSKTKGIGNIRLKTNSGTE 304
             Q ++WVIDS AS H++S    FTS+ +G+ G+VRMGN  +SK  G+G+I L+TN+G +
Sbjct: 269 IGQETDWVIDSGASFHVTSRADFFTSYSQGDFGNVRMGNEDVSKIVGMGDICLETNTGCK 328

Query: 305 LLLRDVRYVPSIKMNLISTGKLDDDVYQSEFGGNQWKLTKGSKLVAIGHRRSTVYTSQLS 349
           LLLRDVR+VP I++NLISTGKLDD+ Y + F   +WKL+KGS +VA G +  ++YT Q  
Sbjct: 329 LLLRDVRHVPDIRLNLISTGKLDDEGYNNNFSDGKWKLSKGSLVVAKGKKTCSLYTVQAK 385

BLAST of MC09g_new0135 vs. ExPASy TrEMBL
Match: A0A4U5PY83 (CCHC-type domain-containing protein OS=Populus alba OX=43335 GN=D5086_0000161380 PE=4 SV=1)

HSP 1 Score: 247 bits (631), Expect = 1.28e-73
Identity = 141/346 (40.75%), Postives = 213/346 (61.56%), Query Frame = 0

Query: 5   WRRMNRQLINI-RLCLSMNVASLVANETTAIDLMKALENRYEKPSANNKVYLVRRLFNIQ 64
           W+ ++RQ++ I RL LS  VA  V  E +   LM+AL   YEKPSANNKV+L+++LFN++
Sbjct: 51  WQLLDRQVLGIIRLSLSRRVAHNVTKEKSTAKLMEALSGMYEKPSANNKVHLMKKLFNLK 110

Query: 65  MEENTSVNSHINKVTKLINQLASVKITFSDEVNAIQLLTSLPESWETMKTAVSNSLGDKS 124
           M ENTSV  H+N    + NQL+SV+I F DE+ A+ LL SLP SWE M+TAVSNS G   
Sbjct: 111 MVENTSVTQHLNNFNTITNQLSSVEIEFDDEIRALILLASLPSSWEGMRTAVSNSAGKSK 170

Query: 125 LKFSEICDAAIAEEIRRKGSGKESTSVQGSALAV-------EKGREKVASDDRQQKRSRR 184
           LK+ +I D  +AEE+RRK SG+ S+S  GSAL +       ++G  +  S  R + +S+ 
Sbjct: 171 LKYDDIRDLILAEEVRRKDSGESSSS--GSALNINTRGRRHDRGSSRGRSKSRYRSKSKF 230

Query: 185 NWKRDAECFHCHKKGHIKKNCRILKEDLKRYMTESNAVVDDALVCVDSNTEIGNQSSEWV 244
             ++  EC++C K GH  +NC   K+        +   V DAL+ V     + +Q  EW+
Sbjct: 231 GSRKQVECWNCGKPGHFSRNCTKPKKPEADSANATTDEVQDALILV-----VQSQIDEWI 290

Query: 245 IDSAASVHISSNRRLFTSFRRGNRGHVRMGNGRLSKTKGIGNIRLKTNSGTELLLRDVRY 304
           +DS AS H + +  +  ++  G+ G V + +G   K  GIG++++KT +G+   L++VR+
Sbjct: 291 LDSGASFHCTPHNEMLQNYVGGDHGVVYLADGTPLKIVGIGDVQIKTMNGSIWTLQNVRH 350

Query: 305 VPSIKMNLISTGKLDDDVYQSEFGGNQWKLTKGSKLVAIGHRRSTV 342
           VP +K  LIS G+LDD  +   F G  WK++KG+ ++A G +  T+
Sbjct: 351 VPELKKKLISVGQLDDSGHSILFAGGMWKVSKGAMVLARGKKTGTL 389

BLAST of MC09g_new0135 vs. ExPASy TrEMBL
Match: A0A4U5QS59 (CCHC-type domain-containing protein OS=Populus alba OX=43335 GN=D5086_0000049350 PE=4 SV=1)

HSP 1 Score: 244 bits (624), Expect = 1.43e-72
Identity = 139/346 (40.17%), Postives = 211/346 (60.98%), Query Frame = 0

Query: 5   WRRMNRQLINI-RLCLSMNVASLVANETTAIDLMKALENRYEKPSANNKVYLVRRLFNIQ 64
           W+ ++RQ++ I RL LS  VA  V  E +   LM+AL   YEKPSANNKV+L+++LFN++
Sbjct: 51  WQLLDRQVLGIIRLSLSRRVAHNVTKEKSTAKLMEALSGMYEKPSANNKVHLMKKLFNLK 110

Query: 65  MEENTSVNSHINKVTKLINQLASVKITFSDEVNAIQLLTSLPESWETMKTAVSNSLGDKS 124
           M ENTSV  H+N    + NQL+SV+I F DE+ A+ LL SLP SWE M+TAVSNS G   
Sbjct: 111 MVENTSVTQHLNNFNTITNQLSSVEIEFDDEIRALILLASLPSSWEGMRTAVSNSAGKSK 170

Query: 125 LKFSEICDAAIAEEIRRKGSGKESTSVQGSALAV-------EKGREKVASDDRQQKRSRR 184
           LK+ +I D  +AEE+RRK SG+ S+S  GSAL +       ++G  +  S  R + +S+ 
Sbjct: 171 LKYDDIRDLILAEEVRRKDSGESSSS--GSALNINTRGRRHDRGSSRGRSKSRYRSKSKF 230

Query: 185 NWKRDAECFHCHKKGHIKKNCRILKEDLKRYMTESNAVVDDALVCVDSNTEIGNQSSEWV 244
             ++  EC++C K GH  +NC   K+        +   V DAL+       + +Q  EW+
Sbjct: 231 GSRKQVECWNCGKPGHFSRNCTKPKKPEADSANATTDEVQDALILA-----VQSQIDEWI 290

Query: 245 IDSAASVHISSNRRLFTSFRRGNRGHVRMGNGRLSKTKGIGNIRLKTNSGTELLLRDVRY 304
           +DS AS H + +  +  ++  G+ G + + +G      GIG++++KT +G+   L++VR+
Sbjct: 291 LDSGASFHCTPHNEMLQNYVGGDHGVLYLADGTPLNIVGIGDVQIKTMNGSIWTLQNVRH 350

Query: 305 VPSIKMNLISTGKLDDDVYQSEFGGNQWKLTKGSKLVAIGHRRSTV 342
           VP +K  LIS G+LDD  Y   F G  WK++KG+ ++A G +  T+
Sbjct: 351 VPELKKKLISVGQLDDSGYSILFAGGMWKVSKGAMVLARGKKTGTL 389

BLAST of MC09g_new0135 vs. ExPASy TrEMBL
Match: A0A2N9G6J0 (CCHC-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS26138 PE=4 SV=1)

HSP 1 Score: 246 bits (627), Expect = 3.75e-72
Identity = 150/364 (41.21%), Postives = 219/364 (60.16%), Query Frame = 0

Query: 5   WRRMNRQLIN-IRLCLSMNVASLVANETTAIDLMKALENRYEKPSANNKVYLVRRLFNIQ 64
           W  ++RQ++  IRL LS +VA  V  ETT + LM AL   YEKPSANNKV+L+++LFN++
Sbjct: 52  WALLDRQVLGVIRLTLSRSVAHNVVKETTTVGLMTALSGMYEKPSANNKVHLMKKLFNLK 111

Query: 65  MEENTSVNSHINKVTKLINQLASVKITFSDEVNAIQLLTSLPESWETMKTAVSNSLGDKS 124
           M E  +V  H+N+   + NQL+SV+I F DE+ A+ +L SLP SWE M+ AVSNS G   
Sbjct: 112 MAEGAAVAKHLNEFNTITNQLSSVEIEFDDEIRALIVLASLPNSWEAMRMAVSNSAGKGK 171

Query: 125 LKFSEICDAAIAEEIRRKGSGKESTSVQGSALAVE-KGREK-------VASDDRQQKRSR 184
           LK+ +I D  ++EE+RR+ +  +  + +G A   E +GR +          +DR + R +
Sbjct: 172 LKYDDIRDLILSEEVRRRDACID--NAEGQAFVTENRGRSRNKWRNNRATFNDRSKSRDK 231

Query: 185 RNWKRDAECFHCHKKGHIKKNCRILK------EDLKRYMTE-SNAVVDDALVCVDSNTE- 244
                  ECFHC KKGHI+ NC+  +      ED K    + + AVVDD  V V S  E 
Sbjct: 232 SQHTETRECFHCGKKGHIRINCKHWRKEQTEDEDHKHDDEKGTTAVVDDEEVVVLSVQEQ 291

Query: 245 ----IGNQSSEWVIDSAASVHISSNRRLFTSFRRGNRGHVRMGNGRLSKTKGIGNIRLKT 304
               + N   EWV+DSAA+ H+   + LFT+++ G+ G V+MGN   SK  GIG++ +KT
Sbjct: 292 KCEHVDNIDDEWVVDSAATHHVVRTKELFTTYKAGDFGTVKMGNTSYSKIVGIGDVCIKT 351

Query: 305 NSGTELLLRDVRYVPSIKMNLISTGKLDDDVYQSEFGGNQWKLTKGSKLVAIGHRRSTVY 347
           N G  ++L++VR+VP +  NLIST  +D   Y +  G  +WKL KG  +VA G     +Y
Sbjct: 352 NVGFTVMLKNVRHVPDLCFNLISTPVMDRAGYCNHLGNGRWKLAKGPMVVARGRICCGLY 411

BLAST of MC09g_new0135 vs. TAIR 10
Match: AT3G21000.1 (Gag-Pol-related retrotransposon family protein )

HSP 1 Score: 48.5 bits (114), Expect = 1.7e-05
Identity = 52/266 (19.55%), Postives = 119/266 (44.74%), Query Frame = 0

Query: 55  LVRRLFNIQMEENTSVNSHINKVTKLINQLASVKITFSDEVNAIQLLTSLPESWETMKTA 114
           L ++L +++M +  S +S+++K  +++ +L   K+  SD      + T+L  S++ +   
Sbjct: 122 LEKQLEDLKMVDKESGSSYLDKALEILERLGRAKLEKSDYEICKNVFTTLSGSFDGL--- 181

Query: 115 VSNSLGDKSLKFSEICDAAIAEEIRRKGSGKESTSVQGSALAVEKGREKVASDDRQQKRS 174
             +S+ ++ +   ++   ++ E    +      +S + +   + K        D + K  
Sbjct: 182 --DSMLEELIDVHKMTSKSLVEYFYYR---VHESSTEEAIFGLLK--------DLRLKSK 241

Query: 175 RRNWKRDAECFHCHKKGHIKKNCRILKEDLKRYMTESNAVVDDALVCVDSNTEIGNQSSE 234
              W     C  C+K  H +++C+  +    +   E   VVD  L  V +          
Sbjct: 242 SEKW-----CGLCYKNNHNQEDCK-FRIHTDKEEKEDEIVVDYRLETVPNLGAKTYDDDI 301

Query: 235 WVIDSAASVHISSNRRLFTSFRRGNRGHVRMGNGRLSKTKGIGNIRLKTNSGTELLLRDV 294
           W+I   A ++++   + FT+  R  +  V   +G +   +G G+++++   G +  +R+V
Sbjct: 302 WIIHKMAPINMTPYVKYFTTLDRTFKATVGTVDGTVLLVEGKGDVKIRMKEGKKKTIRNV 361

Query: 295 RYVPSIKMNLISTGKLDDDVYQSEFG 321
            +VP +  N++S GK+    Y    G
Sbjct: 362 IFVPGLNRNVLSFGKMVSKRYSISTG 365