MC09g_new0007 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g_new0007
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	S-protein homolog
Location	MC09: 404773 .. 405324 (-)
RNA-Seq Expression	MC09g_new0007
Synteny	MC09g_new0007

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTGGGCAAAGTGTGTATATATATTTATTCATGTCCAACATGAATTGCAACAAAGACAGAGAGACAAGTTTTGTTAAGAAAATGGGTCCAATATATCCAACAAATAGGTTTGTGGCTGTTTTGTTGCTGGTATCGGCAGCTCTTGTGGGGGCTCAGACGAAGCCGTCGGGGTCACCGTCGTTGTTGGTGACTCAAAGATGGCATGTTCACGTGGTTAATAATCTAAACAACAATTTCTTGGATGTGCACTGCAAGTCGAAAGACGATGATTTGGGCTATCAACGCTTGGTTCGTGGAGCTGACTTCCAATGGAGCTTTAAAGTTAACTTTTGGGGAACAACTTTGTTTTGGTGTAACTTACACAAGCCAGGTGCCTATGTCACGTTTGAAGTATTTTGGCCTGAATCAAGGAATGCATGGCTTCGTAATAGGTGCCATAATGGAACTGAAGGACATTGCATTTGGACGGCTAAAGATGATGGAATTTACTTGAGAAATATTCCGACTAATTCTGAAGAGTTGATTCATAAGTGGATCAGTTTGAAATAG

mRNA sequence

ATGCTTGGGCAAAGTGTGTATATATATTTATTCATGTCCAACATGAATTGCAACAAAGACAGAGAGACAAGTTTTGTTAAGAAAATGGGTCCAATATATCCAACAAATAGGTTTGTGGCTGTTTTGTTGCTGGTATCGGCAGCTCTTGTGGGGGCTCAGACGAAGCCGTCGGGGTCACCGTCGTTGTTGGTGACTCAAAGATGGCATGTTCACGTGGTTAATAATCTAAACAACAATTTCTTGGATGTGCACTGCAAGTCGAAAGACGATGATTTGGGCTATCAACGCTTGGTTCGTGGAGCTGACTTCCAATGGAGCTTTAAAGTTAACTTTTGGGGAACAACTTTGTTTTGGTGTAACTTACACAAGCCAGGTGCCTATGTCACGTTTGAAGTATTTTGGCCTGAATCAAGGAATGCATGGCTTCGTAATAGGTGCCATAATGGAACTGAAGGACATTGCATTTGGACGGCTAAAGATGATGGAATTTACTTGAGAAATATTCCGACTAATTCTGAAGAGTTGATTCATAAGTGGATCAGTTTGAAATAG

Coding sequence (CDS)

ATGCTTGGGCAAAGTGTGTATATATATTTATTCATGTCCAACATGAATTGCAACAAAGACAGAGAGACAAGTTTTGTTAAGAAAATGGGTCCAATATATCCAACAAATAGGTTTGTGGCTGTTTTGTTGCTGGTATCGGCAGCTCTTGTGGGGGCTCAGACGAAGCCGTCGGGGTCACCGTCGTTGTTGGTGACTCAAAGATGGCATGTTCACGTGGTTAATAATCTAAACAACAATTTCTTGGATGTGCACTGCAAGTCGAAAGACGATGATTTGGGCTATCAACGCTTGGTTCGTGGAGCTGACTTCCAATGGAGCTTTAAAGTTAACTTTTGGGGAACAACTTTGTTTTGGTGTAACTTACACAAGCCAGGTGCCTATGTCACGTTTGAAGTATTTTGGCCTGAATCAAGGAATGCATGGCTTCGTAATAGGTGCCATAATGGAACTGAAGGACATTGCATTTGGACGGCTAAAGATGATGGAATTTACTTGAGAAATATTCCGACTAATTCTGAAGAGTTGATTCATAAGTGGATCAGTTTGAAATAG

Protein sequence

MLGQSVYIYLFMSNMNCNKDRETSFVKKMGPIYPTNRFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHNGTEGHCIWTAKDDGIYLRNIPTNSEELIHKWISLK

Homology

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BLAST of MC09g_new0007 vs. ExPASy Swiss-Prot
Match: F4JLS0 (S-protein homolog 1 OS=Arabidopsis thaliana OX=3702 GN=SPH1 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 3.6e-17
Identity = 47/113 (41.59%), Postives = 62/113 (54.87%), Query Frame = 0


Query: 68  WHVHVVNNL-NNNFLDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGA 127
           W V VVN L     L +HCKSK+DDLG   L     F W+F  N   +T FWC ++K   
Sbjct: 41  WQVTVVNGLTTGETLFIHCKSKEDDLGEINLKFRNRFSWNFGENMLHSTFFWCYMNKDNG 100

Query: 128 YVTFEVFWPESRNAWLRNRCHNGTEGHCIWTAKDDGIYLRNIPTNSEELIHKW 180
           ++   VFW +     L +RC  G + +CIWTAK DG+YL N  +  + L  KW
Sbjct: 101 HMNVNVFWDD---VILFHRC--GWK-NCIWTAKTDGLYLWNSASGEDVLSRKW 147

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BLAST of MC09g_new0007 vs. ExPASy Swiss-Prot
Match: Q2HQ46 (S-protein homolog 74 OS=Arabidopsis thaliana OX=3702 GN=SPH74 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 4.0e-16
Identity = 45/115 (39.13%), Postives = 61/115 (53.04%), Query Frame = 0


Query: 68  WHVHVVNNL-NNNFLDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGA 127
           W V V N L     L +HCKSK++DLG   L     F W+F  N   +TLFWC + K   
Sbjct: 41  WQVTVANGLTTGETLFIHCKSKENDLGDINLKFLDRFSWNFGENMLHSTLFWCYMSKDDG 100

Query: 128 YVTFEVFWPESRNAWLRNRCHNGTEGHCIWTAKDDGIYLRNIPTNSEELIHKWIS 182
           ++  +VFW +     L +RC      +C+WTAK+DG+YL N     + L  KW S
Sbjct: 101 HMNVKVFWDD---VILFHRC---DWKNCVWTAKNDGLYLWNSAIGEDVLSEKWKS 149

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BLAST of MC09g_new0007 vs. ExPASy Swiss-Prot
Match: F2Q9V4 (S-protein homolog 6 OS=Arabidopsis thaliana OX=3702 GN=SPH6 PE=3 SV=2)

HSP 1 Score: 76.3 bits (186), Expect = 4.1e-13
Identity = 44/144 (30.56%), Postives = 66/144 (45.83%), Query Frame = 0


Query: 41  VLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSKDDDLGYQRLVRG 100
           ++ +V  +L+G +T         +       V+ N N+  L VHCKS+DDD G+  L +G
Sbjct: 4   IIFIVLISLIGCETLQHDGKVFPMKGPLTRVVIYNDNDYLLGVHCKSRDDDHGFHILQKG 63

Query: 101 ADFQWSFKVNFWGTTLFWCNLH----KPGAYVTFEVFWPESRNAWLRNRCHNGTEGHCIW 160
             + W F VNF  +TL++C       K G +  ++     S       RC N     C W
Sbjct: 64  GLYGWMFYVNFMNSTLYFCGFSQEQVKKGVFDIYKAVRDSS-------RCRN-----CTW 123

Query: 161 TAKDDGIYLRNIPTNSEELIHKWI 181
            AK+DGIY          L +KW+
Sbjct: 124 EAKEDGIYGYGEIPKKNPLFYKWL 135

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BLAST of MC09g_new0007 vs. ExPASy Swiss-Prot
Match: F4JLQ5 (S-protein homolog 2 OS=Arabidopsis thaliana OX=3702 GN=SPH2 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 9.2e-13
Identity = 40/125 (32.00%), Postives = 66/125 (52.80%), Query Frame = 0


Query: 56  PSGSPSLLVTQRWHVHVVNNLNNNF-LDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGT 115
           PS + S+  T +  V + N+L N   L  HCKSKDDDLG + L  G  + +SF   F+G 
Sbjct: 36  PSSTNSVFPTSKRTVEINNDLGNQLTLLYHCKSKDDDLGNRTLQPGESWSFSFGRQFFGR 95

Query: 116 TLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHNGTEGHCIWTAKDDGIYLRNIPTNSEE 175
           TL++C+   P    +F+++  + R++   N+C +     C+W  + +G    N  T   +
Sbjct: 96  TLYFCSFSWPNESHSFDIY-KDHRDSGGDNKCES---DRCVWKIRRNGPCRFNDETKQFD 155

Query: 176 LIHKW 180
           L + W
Sbjct: 156 LCYPW 156

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BLAST of MC09g_new0007 vs. ExPASy Swiss-Prot
Match: Q9LW22 (S-protein homolog 21 OS=Arabidopsis thaliana OX=3702 GN=SPH21 PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.2e-12
Identity = 38/92 (41.30%), Postives = 48/92 (52.17%), Query Frame = 0


Query: 73  VNNLNNNFLDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEV 132
           +N  N N L VHCKSK++D+G + L  G    +SFK NFWGTT FWCNL+K   Y  +  
Sbjct: 32  LNPKNKNILKVHCKSKNNDIGVKYLKIGEVMSFSFKTNFWGTTEFWCNLYKGPDYKRY-- 91

Query: 133 FWPESRNAWLRNRCHNGTEGHCIWTAKDDGIY 165
                  A+              W A+DDGIY
Sbjct: 92  ---RGITAYQAIGLFAKDGSSYNWLARDDGIY 118

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BLAST of MC09g_new0007 vs. NCBI nr
Match: XP_022143839.1 (S-protein homolog 1-like [Momordica charantia])

HSP 1 Score: 340 bits (872), Expect = 8.11e-118
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 29  MGPIYPTNRFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSK 88
           MGPIYPTNRFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSK
Sbjct: 1   MGPIYPTNRFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSK 60

Query: 89  DDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHN 148
           DDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHN
Sbjct: 61  DDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHN 120

Query: 149 GTEGHCIWTAKDDGIYLRNIPTNSEELIHKWISLK 183
           GTEGHCIWTAKDDGIYLRNIPTNSEELIHKWISLK
Sbjct: 121 GTEGHCIWTAKDDGIYLRNIPTNSEELIHKWISLK 155

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BLAST of MC09g_new0007 vs. NCBI nr
Match: XP_022143852.1 (S-protein homolog 74-like [Momordica charantia])

HSP 1 Score: 228 bits (582), Expect = 9.38e-74
Identity = 104/153 (67.97%), Postives = 118/153 (77.12%), Query Frame = 0


Query: 29  MGPIYPTNRFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSK 88
           MGP YPTNR+V VLLLV  ALVGA   P G        RW+VH+VN LNN+ L VHCKSK
Sbjct: 1   MGPKYPTNRYVVVLLLVMVALVGASGLPLG--------RWNVHIVNKLNNDVLAVHCKSK 60

Query: 89  DDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHN 148
           D+DL YQRLV GADF WSF+VN WGTTLFWCNL K  AY +F+ FWPES+N WLR RC +
Sbjct: 61  DNDLRYQRLVPGADFHWSFRVNVWGTTLFWCNLQKSSAYASFDTFWPESKNQWLRYRCTD 120

Query: 149 GTEGHCIWTAKDDGIYLRNIPTNSEELIHKWIS 181
            T+G+CIWTAKDDGIYL+NIP N EEL+HKW S
Sbjct: 121 RTKGNCIWTAKDDGIYLKNIPDNVEELVHKWTS 145

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BLAST of MC09g_new0007 vs. NCBI nr
Match: XP_022991767.1 (S-protein homolog 74-like [Cucurbita maxima])

HSP 1 Score: 192 bits (488), Expect = 1.87e-59
Identity = 91/146 (62.33%), Postives = 105/146 (71.92%), Query Frame = 0


Query: 39  VAVLLLVSAALVGAQTKPSGSPSLLVT---QRWHVHVVNNLNNNFLDVHCKSKDDDLGYQ 98
           V VLLL   A VGAQ      P ++      R++VHV N L+   L VHC+SKDDDLG  
Sbjct: 5   VVVLLLGMVAFVGAQV-----PGIIYQPKPSRYYVHVANGLSTLPLTVHCQSKDDDLGIH 64

Query: 99  RLV-RGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHNGTEGHC 158
            L  RG DFQW+FKVNFWGTTLFWC L +P AYVTFE FWPES+N WLR+RC NGT+G C
Sbjct: 65  HLPNRGDDFQWNFKVNFWGTTLFWCRLERPDAYVTFETFWPESKNTWLRDRCRNGTQGTC 124

Query: 159 IWTAKDDGIYLRNIPTNSEELIHKWI 180
           +W AKDDGIYL N P N++ELIHKWI
Sbjct: 125 LWIAKDDGIYLFNFPANNDELIHKWI 145

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BLAST of MC09g_new0007 vs. NCBI nr
Match: XP_038896423.1 (S-protein homolog 1-like [Benincasa hispida])

HSP 1 Score: 192 bits (487), Expect = 2.75e-59
Identity = 95/150 (63.33%), Postives = 112/150 (74.67%), Query Frame = 0


Query: 37  RFVAVLLLVSAALVGAQ----TKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSKDDDL 96
           R V VL L +AALVGAQ    + P   PS     R++VHVVN L+N  + VHC+SKDDDL
Sbjct: 2   RNVVVLWLATAALVGAQIPRWSVPPPQPS-----RYYVHVVNGLSNADMVVHCQSKDDDL 61

Query: 97  GYQRLV-RGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPES-RNAWLRNRCHNGT 156
           GY  LV RG D+QW+FK NFWGTTLFWC L KP AYV+FE FWPES +N WLR+RC  GT
Sbjct: 62  GYHHLVNRGDDYQWNFKENFWGTTLFWCKLEKPNAYVSFESFWPESFKNTWLRDRC--GT 121

Query: 157 EGHCIWTAKDDGIYLRNIPTNSEELIHKWI 180
           +G CIWTAKDDGIYLRN+P N +E +HKW+
Sbjct: 122 QGTCIWTAKDDGIYLRNMPINVDEFVHKWL 144

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BLAST of MC09g_new0007 vs. NCBI nr
Match: XP_016903452.1 (PREDICTED: uncharacterized protein LOC103503421 [Cucumis melo] >KAA0040491.1 pumilio-like protein 15-like [Cucumis melo var. makuwa] >TYK23471.1 pumilio-like protein 15-like [Cucumis melo var. makuwa])

HSP 1 Score: 191 bits (484), Expect = 7.85e-59
Identity = 90/145 (62.07%), Postives = 106/145 (73.10%), Query Frame = 0


Query: 37  RFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSKDDDLGYQR 96
           R V VL L    LV  Q      P  L + R+ VHVVN L+   LDVHC+SKDDDLGY R
Sbjct: 4   RNVVVLWLAMMVLVEGQVSKWSKPPPLPS-RYFVHVVNGLSYQSLDVHCQSKDDDLGYHR 63

Query: 97  LVR-GADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHNGTEGHCI 156
           LV+ G +FQW+F+ NFWGTTLFWC L KP AYV FE FWPE++N WLR+RC  G +G CI
Sbjct: 64  LVKQGDEFQWNFEENFWGTTLFWCRLEKPDAYVAFESFWPETKNVWLRHRC--GNQGTCI 123

Query: 157 WTAKDDGIYLRNIPTNSEELIHKWI 180
           WTAKDDGIYLRN+PTN++E +HKWI
Sbjct: 124 WTAKDDGIYLRNMPTNADEFVHKWI 145

[Close]

BLAST of MC09g_new0007 vs. ExPASy TrEMBL
Match: A0A6J1CQI8 (S-protein homolog OS=Momordica charantia OX=3673 GN=LOC111013653 PE=3 SV=1)

HSP 1 Score: 340 bits (872), Expect = 3.93e-118
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 29  MGPIYPTNRFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSK 88
           MGPIYPTNRFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSK
Sbjct: 1   MGPIYPTNRFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSK 60

Query: 89  DDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHN 148
           DDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHN
Sbjct: 61  DDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHN 120

Query: 149 GTEGHCIWTAKDDGIYLRNIPTNSEELIHKWISLK 183
           GTEGHCIWTAKDDGIYLRNIPTNSEELIHKWISLK
Sbjct: 121 GTEGHCIWTAKDDGIYLRNIPTNSEELIHKWISLK 155

[Close]

BLAST of MC09g_new0007 vs. ExPASy TrEMBL
Match: A0A6J1CS23 (S-protein homolog OS=Momordica charantia OX=3673 GN=LOC111013665 PE=3 SV=1)

HSP 1 Score: 228 bits (582), Expect = 4.54e-74
Identity = 104/153 (67.97%), Postives = 118/153 (77.12%), Query Frame = 0


Query: 29  MGPIYPTNRFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSK 88
           MGP YPTNR+V VLLLV  ALVGA   P G        RW+VH+VN LNN+ L VHCKSK
Sbjct: 1   MGPKYPTNRYVVVLLLVMVALVGASGLPLG--------RWNVHIVNKLNNDVLAVHCKSK 60

Query: 89  DDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHN 148
           D+DL YQRLV GADF WSF+VN WGTTLFWCNL K  AY +F+ FWPES+N WLR RC +
Sbjct: 61  DNDLRYQRLVPGADFHWSFRVNVWGTTLFWCNLQKSSAYASFDTFWPESKNQWLRYRCTD 120

Query: 149 GTEGHCIWTAKDDGIYLRNIPTNSEELIHKWIS 181
            T+G+CIWTAKDDGIYL+NIP N EEL+HKW S
Sbjct: 121 RTKGNCIWTAKDDGIYLKNIPDNVEELVHKWTS 145

[Close]

BLAST of MC09g_new0007 vs. ExPASy TrEMBL
Match: A0A6J1JMS5 (S-protein homolog OS=Cucurbita maxima OX=3661 GN=LOC111488300 PE=3 SV=1)

HSP 1 Score: 192 bits (488), Expect = 9.07e-60
Identity = 91/146 (62.33%), Postives = 105/146 (71.92%), Query Frame = 0


Query: 39  VAVLLLVSAALVGAQTKPSGSPSLLVT---QRWHVHVVNNLNNNFLDVHCKSKDDDLGYQ 98
           V VLLL   A VGAQ      P ++      R++VHV N L+   L VHC+SKDDDLG  
Sbjct: 5   VVVLLLGMVAFVGAQV-----PGIIYQPKPSRYYVHVANGLSTLPLTVHCQSKDDDLGIH 64

Query: 99  RLV-RGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHNGTEGHC 158
            L  RG DFQW+FKVNFWGTTLFWC L +P AYVTFE FWPES+N WLR+RC NGT+G C
Sbjct: 65  HLPNRGDDFQWNFKVNFWGTTLFWCRLERPDAYVTFETFWPESKNTWLRDRCRNGTQGTC 124

Query: 159 IWTAKDDGIYLRNIPTNSEELIHKWI 180
           +W AKDDGIYL N P N++ELIHKWI
Sbjct: 125 LWIAKDDGIYLFNFPANNDELIHKWI 145

[Close]

BLAST of MC09g_new0007 vs. ExPASy TrEMBL
Match: A0A5D3DIG9 (S-protein homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold278G00380 PE=3 SV=1)

HSP 1 Score: 191 bits (484), Expect = 3.80e-59
Identity = 90/145 (62.07%), Postives = 106/145 (73.10%), Query Frame = 0


Query: 37  RFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSKDDDLGYQR 96
           R V VL L    LV  Q      P  L + R+ VHVVN L+   LDVHC+SKDDDLGY R
Sbjct: 4   RNVVVLWLAMMVLVEGQVSKWSKPPPLPS-RYFVHVVNGLSYQSLDVHCQSKDDDLGYHR 63

Query: 97  LVR-GADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHNGTEGHCI 156
           LV+ G +FQW+F+ NFWGTTLFWC L KP AYV FE FWPE++N WLR+RC  G +G CI
Sbjct: 64  LVKQGDEFQWNFEENFWGTTLFWCRLEKPDAYVAFESFWPETKNVWLRHRC--GNQGTCI 123

Query: 157 WTAKDDGIYLRNIPTNSEELIHKWI 180
           WTAKDDGIYLRN+PTN++E +HKWI
Sbjct: 124 WTAKDDGIYLRNMPTNADEFVHKWI 145

[Close]

BLAST of MC09g_new0007 vs. ExPASy TrEMBL
Match: A0A1S4E5F6 (S-protein homolog OS=Cucumis melo OX=3656 GN=LOC103503421 PE=3 SV=1)

HSP 1 Score: 191 bits (484), Expect = 3.80e-59
Identity = 90/145 (62.07%), Postives = 106/145 (73.10%), Query Frame = 0


Query: 37  RFVAVLLLVSAALVGAQTKPSGSPSLLVTQRWHVHVVNNLNNNFLDVHCKSKDDDLGYQR 96
           R V VL L    LV  Q      P  L + R+ VHVVN L+   LDVHC+SKDDDLGY R
Sbjct: 4   RNVVVLWLAMMVLVEGQVSKWSKPPPLPS-RYFVHVVNGLSYQSLDVHCQSKDDDLGYHR 63

Query: 97  LVR-GADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHNGTEGHCI 156
           LV+ G +FQW+F+ NFWGTTLFWC L KP AYV FE FWPE++N WLR+RC  G +G CI
Sbjct: 64  LVKQGDEFQWNFEENFWGTTLFWCRLEKPDAYVAFESFWPETKNVWLRHRC--GNQGTCI 123

Query: 157 WTAKDDGIYLRNIPTNSEELIHKWI 180
           WTAKDDGIYLRN+PTN++E +HKWI
Sbjct: 124 WTAKDDGIYLRNMPTNADEFVHKWI 145

[Close]

BLAST of MC09g_new0007 vs. TAIR 10
Match: AT4G16295.1 (S-protein homologue 1 )

HSP 1 Score: 89.7 bits (221), Expect = 2.6e-18
Identity = 47/113 (41.59%), Postives = 62/113 (54.87%), Query Frame = 0


Query: 68  WHVHVVNNL-NNNFLDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGA 127
           W V VVN L     L +HCKSK+DDLG   L     F W+F  N   +T FWC ++K   
Sbjct: 41  WQVTVVNGLTTGETLFIHCKSKEDDLGEINLKFRNRFSWNFGENMLHSTFFWCYMNKDNG 100

Query: 128 YVTFEVFWPESRNAWLRNRCHNGTEGHCIWTAKDDGIYLRNIPTNSEELIHKW 180
           ++   VFW +     L +RC  G + +CIWTAK DG+YL N  +  + L  KW
Sbjct: 101 HMNVNVFWDD---VILFHRC--GWK-NCIWTAKTDGLYLWNSASGEDVLSRKW 147

[Close]

BLAST of MC09g_new0007 vs. TAIR 10
Match: AT4G29035.1 (Plant self-incompatibility protein S1 family )

HSP 1 Score: 86.3 bits (212), Expect = 2.8e-17
Identity = 45/115 (39.13%), Postives = 61/115 (53.04%), Query Frame = 0


Query: 68  WHVHVVNNL-NNNFLDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGA 127
           W V V N L     L +HCKSK++DLG   L     F W+F  N   +TLFWC + K   
Sbjct: 41  WQVTVANGLTTGETLFIHCKSKENDLGDINLKFLDRFSWNFGENMLHSTLFWCYMSKDDG 100

Query: 128 YVTFEVFWPESRNAWLRNRCHNGTEGHCIWTAKDDGIYLRNIPTNSEELIHKWIS 182
           ++  +VFW +     L +RC      +C+WTAK+DG+YL N     + L  KW S
Sbjct: 101 HMNVKVFWDD---VILFHRC---DWKNCVWTAKNDGLYLWNSAIGEDVLSEKWKS 149

[Close]

BLAST of MC09g_new0007 vs. TAIR 10
Match: AT4G16195.1 (Plant self-incompatibility protein S1 family )

HSP 1 Score: 75.1 bits (183), Expect = 6.5e-14
Identity = 40/125 (32.00%), Postives = 66/125 (52.80%), Query Frame = 0


Query: 56  PSGSPSLLVTQRWHVHVVNNLNNNF-LDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGT 115
           PS + S+  T +  V + N+L N   L  HCKSKDDDLG + L  G  + +SF   F+G 
Sbjct: 36  PSSTNSVFPTSKRTVEINNDLGNQLTLLYHCKSKDDDLGNRTLQPGESWSFSFGRQFFGR 95

Query: 116 TLFWCNLHKPGAYVTFEVFWPESRNAWLRNRCHNGTEGHCIWTAKDDGIYLRNIPTNSEE 175
           TL++C+   P    +F+++  + R++   N+C +     C+W  + +G    N  T   +
Sbjct: 96  TLYFCSFSWPNESHSFDIY-KDHRDSGGDNKCES---DRCVWKIRRNGPCRFNDETKQFD 155

Query: 176 LIHKW 180
           L + W
Sbjct: 156 LCYPW 156

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BLAST of MC09g_new0007 vs. TAIR 10
Match: AT3G26880.1 (Plant self-incompatibility protein S1 family )

HSP 1 Score: 74.7 bits (182), Expect = 8.6e-14
Identity = 38/92 (41.30%), Postives = 48/92 (52.17%), Query Frame = 0


Query: 73  VNNLNNNFLDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEV 132
           +N  N N L VHCKSK++D+G + L  G    +SFK NFWGTT FWCNL+K   Y  +  
Sbjct: 32  LNPKNKNILKVHCKSKNNDIGVKYLKIGEVMSFSFKTNFWGTTEFWCNLYKGPDYKRY-- 91

Query: 133 FWPESRNAWLRNRCHNGTEGHCIWTAKDDGIY 165
                  A+              W A+DDGIY
Sbjct: 92  ---RGITAYQAIGLFAKDGSSYNWLARDDGIY 118

[Close]

BLAST of MC09g_new0007 vs. TAIR 10
Match: AT5G06030.1 (Plant self-incompatibility protein S1 family )

HSP 1 Score: 70.1 bits (170), Expect = 2.1e-12
Identity = 38/110 (34.55%), Postives = 57/110 (51.82%), Query Frame = 0


Query: 73  VNNLNNNFLDVHCKSKDDDLGYQRLVRGADFQWSFKVNFWGTTLFWCNLHKPGAYVTFEV 132
           + N N+  L +HCKS+DDDLG+  L +G  F W F VNF  +TL +C   +        +
Sbjct: 50  IQNYNDYLLAIHCKSRDDDLGFHILAKGELFGWKFHVNFRYSTLCFCGFSQRQINKGVFI 109

Query: 133 FWPESRNAWLRNRCHNGTEGHCIWTAKDDGIY-LRNIPTNSEELIHKWIS 182
            +  SR+ +   RC N     C W A+ DG +   +IPT    L + W++
Sbjct: 110 IYVASRDFY---RCAN-----CTWKAEKDGFHGYGDIPTRG-YLFYNWLN 150

The following BLAST results are available for this feature:

BLAST of MC09g_new0007 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JLS0	3.6e-17	41.59	S-protein homolog 1 OS=Arabidopsis thaliana OX=3702 GN=SPH1 PE=2 SV=1	[more]
Q2HQ46	4.0e-16	39.13	S-protein homolog 74 OS=Arabidopsis thaliana OX=3702 GN=SPH74 PE=2 SV=1	[more]
F2Q9V4	4.1e-13	30.56	S-protein homolog 6 OS=Arabidopsis thaliana OX=3702 GN=SPH6 PE=3 SV=2	[more]
F4JLQ5	9.2e-13	32.00	S-protein homolog 2 OS=Arabidopsis thaliana OX=3702 GN=SPH2 PE=3 SV=1	[more]
Q9LW22	1.2e-12	41.30	S-protein homolog 21 OS=Arabidopsis thaliana OX=3702 GN=SPH21 PE=3 SV=1	[more]

BLAST of MC09g_new0007 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143839.1	8.11e-118	100.00	S-protein homolog 1-like [Momordica charantia]	[more]
XP_022143852.1	9.38e-74	67.97	S-protein homolog 74-like [Momordica charantia]	[more]
XP_022991767.1	1.87e-59	62.33	S-protein homolog 74-like [Cucurbita maxima]	[more]
XP_038896423.1	2.75e-59	63.33	S-protein homolog 1-like [Benincasa hispida]	[more]
XP_016903452.1	7.85e-59	62.07	PREDICTED: uncharacterized protein LOC103503421 [Cucumis melo] >KAA0040491.1 pum...	[more]

BLAST of MC09g_new0007 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQI8	3.93e-118	100.00	S-protein homolog OS=Momordica charantia OX=3673 GN=LOC111013653 PE=3 SV=1	[more]
A0A6J1CS23	4.54e-74	67.97	S-protein homolog OS=Momordica charantia OX=3673 GN=LOC111013665 PE=3 SV=1	[more]
A0A6J1JMS5	9.07e-60	62.33	S-protein homolog OS=Cucurbita maxima OX=3661 GN=LOC111488300 PE=3 SV=1	[more]
A0A5D3DIG9	3.80e-59	62.07	S-protein homolog OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold278G00...	[more]
A0A1S4E5F6	3.80e-59	62.07	S-protein homolog OS=Cucumis melo OX=3656 GN=LOC103503421 PE=3 SV=1	[more]

BLAST of MC09g_new0007 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16295.1	2.6e-18	41.59	S-protein homologue 1	[more]
AT4G29035.1	2.8e-17	39.13	Plant self-incompatibility protein S1 family	[more]
AT4G16195.1	6.5e-14	32.00	Plant self-incompatibility protein S1 family	[more]
AT3G26880.1	8.6e-14	41.30	Plant self-incompatibility protein S1 family	[more]
AT5G06030.1	2.1e-12	34.55	Plant self-incompatibility protein S1 family	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010264	Plant self-incompatibility S1	PFAM	PF05938	Self-incomp_S1	coord: 69..179 e-value: 4.2E-27 score: 94.7
IPR010264	Plant self-incompatibility S1	PANTHER	PTHR31232	FAMILY NOT NAMED	coord: 39..180
None	No IPR available	PANTHER	PTHR31232:SF93	BINDING PROTEIN, PUTATIVE-RELATED	coord: 39..180

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g_new0007.1	MC09g_new0007.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0060320	rejection of self pollen
cellular_component	GO:0005576	extracellular region