MC09g1914 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g1914
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionpeptide chain release factor 1
LocationMC09: 23831920 .. 23834950 (+)
RNA-Seq ExpressionMC09g1914
SyntenyMC09g1914
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAATTAATCGAGGGACTCTGCGGCTTGTTGAACAAATCCGTACACTTGCTCCACATTGGCGCACCAATCCTGTACAAAACACTCCCAACCCCTTTCTTCCACCACCGTTCCAACCCCTTTCAACCCCAACTTTTCGTTTTTATTCTAAAGGTTCTTATTTTCTTTCCCTTTTATTCATTTCTACGTTCTCTTCCTTGCGATCAAGTTAATCACAGCTTACTAACGCCTTTATTGAATTAGACCTCTTCGCCCTTTTTATATTCGGATGTTGTGGATTATTATTATTATTATTTTTCCTTTGGGTTCTGCTTCTGAAATATATAACCAACTGAGTACGATATAATTATTAGTGATTACCATTTGATCGAAATCTTTGTTTGTATTGTTGGATGCTCACCCTCCTATAAATCTCAAGATGCGAACACTTTCTTCAAACCTTCTCTAAAGCAGGTGAAATACAACCGCAGCAGCTATCAATGGAGCTTATCAACATAATGGAACATAGGTTGAAGGCCATTGAGCTCAGGAGCACTTTTCTTCTAAATATAATCAACCGGGTACCACATATTCTCACTTTCTTTATTTTGTGTATGCTCTTCATATAGCTTCGAGATTCATTCTTTTTCTTTTTTTCTTTTTTGGAATTCAACATGTGCACATGGGGTGGGATTTCGTAACTCTCTATGCCTTAATCAGTTGATCTGGCACAATTCAGTTTTAAGTTGACGTATTATAAGTAGTACAAAAAATTAATTGTGGTAAAACTTGATTAATGAATCAAAATGGAACTTCATAAATCCTAGATTTTATCTTCGCCTCCTCCATTTCTCCTGGTATTTTTTCTGTACCTCTTTCTCTTTTAATTCAGACCTCAAATGGTGATTTAATTTGCTTTGCTCATGGTATTAATTGAAGATAGCCAGAAGCCCCACCAGTTGAGTACGCAAAGGCCAACAAGGAGCTTAGAAAACTCAGAGATACGATGGATCTCATAAATGAGTTGAGGTCCAGACAGAAGGTAAAATAAGTTTATTCTGGAATACAATGAGTGTTGTCTTTCTTTACAACACAAACTTCTGAAAAGATGAAGACTATTGTTGTTGCCTTTAGTGCAGGATGATCTCTTTCCATGTAGTTCTTTCACACTCATCTACCGGCTTGGTTGTTGTCCAAATTCCAAAATGTTTTAAGTGAAACTTGTATGTGTGTTTTGTGTCTCACATTGAGATTGTGGACGCCTATCTGAATTTCTTGTAAGTGGGAAGTCGGGACTGGCTTGGAGGCATCCGCTTCCCCAATTAATGAAATGTGTGTGGTGTCGTAATATTTACAGAAGGAAATCAGAAGTTCTTTATGACTGTGTTTCTCGCTTGCAGGAAATTAATGGTTTGAGAACGTTGATGACGGAGTGCCCAGAGGATAAAGACATGCTTGATATGGCAACTGAGGAATTGGATCAGGCAGTTGAAGAAGAGAAACGAGTACAGAACTTGTTGCTCAAGTCATTACTCCCCAAGGATGATGCTGATGAGAGGGATTGCATCTTGGAGGTGAGAGCAGGTAGGCAACGATGCATATTAATGAACAAGGACTGTGGACATTACTAAATTTATGGGAGTAGTTTTCATGTTGTGAAGAGGTATGTTTTCTGTCTCCTTTTCTGTTTACGCTGTAGCTAATATAAAGTTTTACAGGAACTGGTGGAGAAGAAGCCTCTTTGTTTGCCATGGATATATTTAAAATGTGAGCGACTATACAATGTTTGGCATTTCTGGTTATCATCAATTCACTAGTTCTAGTTCTTAAAGGTCATTTTGTTTGGGTTGAAATAGGTATGAGAGATACACACAGAGGAAAGGTTGGAAGTTTGAGGTGGTAGACATAACAGAATCGGAACTGAAAGGATATAAGGTGATTTTATGGCAAATTTGAACTACCTTTGAAGTAATGTCTTTTATGTTTATCGGTCCTACACTGCAGGAGGCTGTTGCTGCAATATCAGGAGCTGGGGCTTATGGAAAACTTAAATTTGAGAGTGGAATTCACCGAGTACAGGTTTTTTCTACATGCATATCATTTAGCCTTCTTATCTAACGTTGTTTATCAGAGGCAGGAATGGTAATTGAAACTAGTATCTTCCTTGGGCAGCGAGTTCCTGTGACAGAGAAGTCTGGGCGTGTTCACACCAGTGCTGTTTCTGTAGCCATTCTCCCTCAAGCCGACGAGGTTATAACCCTTCTGGCTTGACGCTTTTAAGCCACTTTCTTCCAGTTTGCTGTTGCAGCGAAATACCTGGAAAATGATTTTGAAAAAGTCATTTCTTAAGTAGAAGGAAAAGGATTATGGTTGTGAATCTGATATGGCTGGGGGGGATGTGCAGGTAGATGTGGAGTTGAAGAACGAGGACCTGAGAATCGATACGTTCAGATCAGGGGGTTCAGGAGGTCAGCATGCAAATACCACAAATAGTGCTGTTAGGGTGACTCACATTCCAACTGGACTGAGTGTGTCCATACAAGACGAGCGATCCCAACATATGGTATGAAATGTAGCATTTCCATTGCTTGTATGATTTTGATGGGGTATTATTGTTTAACAACTTGTTTGTGGTCTTGTTTGGCTCCAACAGAACAAGGCAAAGGCGCTTAAAGTAATATGCGCAAAGCTATATGAAAAGGAAAGGTGCAGAATTCAGAGCAGTCGGTCGAAGCTGCGATCGGAACAGGTACAAATAATTGATGGATGTATGATGTTGGTTGTATGCATGTAGTTACTGCTGTGTTGGAATAATATACAAAATAGTGAAGTGAAGTAAGTATGGTTGATGATAAGCAGATTGGGAGTGGGGACAGATCGGAACGCATTCGCACATACAACTTCCCGCAAGGGCGGGTAACTGACCATCGGGTAGGGATCACAGAGCATGCAATCGAGGAGGTGATGCAAGGACAGAATCTGGATATGTTCGTGGACGCTCTGTTGTTGCAGCAGGAAATGGACGCAATAGCATCCTTCACTTCTACTTCG

mRNA sequence

ATGAGAATTAATCGAGGGACTCTGCGGCTTGTTGAACAAATCCGTACACTTGCTCCACATTGGCGCACCAATCCTGTACAAAACACTCCCAACCCCTTTCTTCCACCACCGTTCCAACCCCTTTCAACCCCAACTTTTCGTTTTTATTCTAAAGGTGAAATACAACCGCAGCAGCTATCAATGGAGCTTATCAACATAATGGAACATAGGTTGAAGGCCATTGAGCTCAGGAGCACTTTTCTTCTAAATATAATCAACCGGCCAGAAGCCCCACCAGTTGAGTACGCAAAGGCCAACAAGGAGCTTAGAAAACTCAGAGATACGATGGATCTCATAAATGAGTTGAGGTCCAGACAGAAGGAAATTAATGGTTTGAGAACGTTGATGACGGAGTGCCCAGAGGATAAAGACATGCTTGATATGGCAACTGAGGAATTGGATCAGGCAGTTGAAGAAGAGAAACGAGTACAGAACTTGTTGCTCAAGTCATTACTCCCCAAGGATGATGCTGATGAGAGGGATTGCATCTTGGAGGTGAGAGCAGGAACTGGTGGAGAAGAAGCCTCTTTGTTTGCCATGGATATATTTAAAATGTATGAGAGATACACACAGAGGAAAGGTTGGAAGTTTGAGGTGGTAGACATAACAGAATCGGAACTGAAAGGATATAAGGAGGCTGTTGCTGCAATATCAGGAGCTGGGGCTTATGGAAAACTTAAATTTGAGAGTGGAATTCACCGAGTACAGCGAGTTCCTGTGACAGAGAAGTCTGGGCGTGTTCACACCAGTGCTGTTTCTGTAGCCATTCTCCCTCAAGCCGACGAGGTAGATGTGGAGTTGAAGAACGAGGACCTGAGAATCGATACGTTCAGATCAGGGGGTTCAGGAGGTCAGCATGCAAATACCACAAATAGTGCTGTTAGGGTGACTCACATTCCAACTGGACTGAGTGTGTCCATACAAGACGAGCGATCCCAACATATGAACAAGGCAAAGGCGCTTAAAGTAATATGCGCAAAGCTATATGAAAAGGAAAGGTGCAGAATTCAGAGCAGTCGGTCGAAGCTGCGATCGGAACAGATTGGGAGTGGGGACAGATCGGAACGCATTCGCACATACAACTTCCCGCAAGGGCGGGTAACTGACCATCGGGTAGGGATCACAGAGCATGCAATCGAGGAGGTGATGCAAGGACAGAATCTGGATATGTTCGTGGACGCTCTGTTGTTGCAGCAGGAAATGGACGCAATAGCATCCTTCACTTCTACTTCG

Coding sequence (CDS)

ATGAGAATTAATCGAGGGACTCTGCGGCTTGTTGAACAAATCCGTACACTTGCTCCACATTGGCGCACCAATCCTGTACAAAACACTCCCAACCCCTTTCTTCCACCACCGTTCCAACCCCTTTCAACCCCAACTTTTCGTTTTTATTCTAAAGGTGAAATACAACCGCAGCAGCTATCAATGGAGCTTATCAACATAATGGAACATAGGTTGAAGGCCATTGAGCTCAGGAGCACTTTTCTTCTAAATATAATCAACCGGCCAGAAGCCCCACCAGTTGAGTACGCAAAGGCCAACAAGGAGCTTAGAAAACTCAGAGATACGATGGATCTCATAAATGAGTTGAGGTCCAGACAGAAGGAAATTAATGGTTTGAGAACGTTGATGACGGAGTGCCCAGAGGATAAAGACATGCTTGATATGGCAACTGAGGAATTGGATCAGGCAGTTGAAGAAGAGAAACGAGTACAGAACTTGTTGCTCAAGTCATTACTCCCCAAGGATGATGCTGATGAGAGGGATTGCATCTTGGAGGTGAGAGCAGGAACTGGTGGAGAAGAAGCCTCTTTGTTTGCCATGGATATATTTAAAATGTATGAGAGATACACACAGAGGAAAGGTTGGAAGTTTGAGGTGGTAGACATAACAGAATCGGAACTGAAAGGATATAAGGAGGCTGTTGCTGCAATATCAGGAGCTGGGGCTTATGGAAAACTTAAATTTGAGAGTGGAATTCACCGAGTACAGCGAGTTCCTGTGACAGAGAAGTCTGGGCGTGTTCACACCAGTGCTGTTTCTGTAGCCATTCTCCCTCAAGCCGACGAGGTAGATGTGGAGTTGAAGAACGAGGACCTGAGAATCGATACGTTCAGATCAGGGGGTTCAGGAGGTCAGCATGCAAATACCACAAATAGTGCTGTTAGGGTGACTCACATTCCAACTGGACTGAGTGTGTCCATACAAGACGAGCGATCCCAACATATGAACAAGGCAAAGGCGCTTAAAGTAATATGCGCAAAGCTATATGAAAAGGAAAGGTGCAGAATTCAGAGCAGTCGGTCGAAGCTGCGATCGGAACAGATTGGGAGTGGGGACAGATCGGAACGCATTCGCACATACAACTTCCCGCAAGGGCGGGTAACTGACCATCGGGTAGGGATCACAGAGCATGCAATCGAGGAGGTGATGCAAGGACAGAATCTGGATATGTTCGTGGACGCTCTGTTGTTGCAGCAGGAAATGGACGCAATAGCATCCTTCACTTCTACTTCG

Protein sequence

MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKGEIQPQQLSMELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQKEINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVRAGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLKFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHANTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASFTSTS
Homology
BLAST of MC09g1914 vs. ExPASy Swiss-Prot
Match: A2RVR7 (Peptide chain release factor 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g47020 PE=2 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 9.2e-165
Identity = 296/384 (77.08%), Postives = 349/384 (90.89%), Query Frame = 0

Query: 41  LSTPTFRFYSKGEIQPQQLSMELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANK 100
           LS+   R Y+ G ++P QLS +LI IM+ RL AIE R+  L  +IN+PE  P E+++ANK
Sbjct: 32  LSSNMIRLYTTG-MEP-QLSPDLIKIMDQRLSAIEHRNAVLQKLINQPEYSPEEFSRANK 91

Query: 101 ELRKLRDTMDLINELRSRQKEINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLL 160
           ELRKLRD+M LIN+LR++QKEI+GL++L++E  +DKDMLD+A  ELD+AVEEEKR+Q LL
Sbjct: 92  ELRKLRDSMLLINDLRAKQKEIDGLKSLVSESSDDKDMLDLAVGELDEAVEEEKRLQTLL 151

Query: 161 LKSLLPKDDADERDCILEVRAGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESEL 220
           LKSLLPKD+ADERDCILEVRAGTGGEEASLFAMDIF+MYERY+Q+KGWKF++VDITES++
Sbjct: 152 LKSLLPKDEADERDCILEVRAGTGGEEASLFAMDIFRMYERYSQKKGWKFDIVDITESDM 211

Query: 221 KGYKEAVAAISGAGAYGKLKFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVEL 280
           KGYKEA AAI GA  YGKLKFESGIHRVQR+P+TEKSGR+HTSA+SVAILPQADEVDV+L
Sbjct: 212 KGYKEASAAICGASVYGKLKFESGIHRVQRIPITEKSGRIHTSAISVAILPQADEVDVQL 271

Query: 281 KNEDLRIDTFRSGGSGGQHANTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAK 340
           +NEDLRIDT+RSGGSGGQHANTTNSAVR+ H+PTG+ VSIQDERSQHMN+AKALKV+CA+
Sbjct: 272 RNEDLRIDTYRSGGSGGQHANTTNSAVRIIHLPTGMMVSIQDERSQHMNRAKALKVLCAR 331

Query: 341 LYEKERCRIQSSRSKLRSEQIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQN 400
           LYE ER RIQSSRSKLRS+QIGSGDRS RIRTYNFPQGRVTDHRVGIT HAIE++MQG+N
Sbjct: 332 LYEIERLRIQSSRSKLRSDQIGSGDRSGRIRTYNFPQGRVTDHRVGITHHAIEDMMQGEN 391

Query: 401 LDMFVDALLLQQEMDAIASFTSTS 425
           LDMF+DALLL+QEMDAIASF+STS
Sbjct: 392 LDMFIDALLLRQEMDAIASFSSTS 413

BLAST of MC09g1914 vs. ExPASy Swiss-Prot
Match: A5V9C3 (Peptide chain release factor 1 OS=Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273) OX=392499 GN=prfA PE=3 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 4.2e-93
Identity = 172/356 (48.31%), Postives = 246/356 (69.10%), Query Frame = 0

Query: 63  LINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQKEI 122
           +  I   R+  IE R   L  ++ R + P  ++ + +K+  ++        E+R  + E+
Sbjct: 1   MAGITSARIAQIEARRDELQALMARGDLPSDQFVQLSKDYAEIEPVAQAAGEVRRLRAEL 60

Query: 123 NGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVRAG 182
           + L+ +  +   D ++  MA EE ++        +  L   LLP+D ADER  +LE+RAG
Sbjct: 61  DVLKGMAEDEAGDAELRAMAIEEAEEIRARLPEAERALALKLLPRDAADERSAMLEIRAG 120

Query: 183 TGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLKFE 242
           TGG+EA+LFA D+F+MY+RY + +GW+ E++  + ++L GYKE VA+++GAG + +LKFE
Sbjct: 121 TGGDEAALFAGDLFRMYQRYAEDRGWRVELISASAADLGGYKEVVASVTGAGVFARLKFE 180

Query: 243 SGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHANT 302
           SG+HRVQRVPVTE  GR+HTSA +VA+LP+A+EVDV + ++DLRID +RS G GGQ  NT
Sbjct: 181 SGVHRVQRVPVTESGGRIHTSAATVAVLPEAEEVDVHIDDKDLRIDVYRSSGPGGQSVNT 240

Query: 303 TNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQIG 362
           T+SAVR+TH+PTGL V  QDE+SQH NKAKALKV+  +LYE ER R+ S R+  R   +G
Sbjct: 241 TDSAVRITHLPTGLVVIQQDEKSQHKNKAKALKVLRTRLYEMERERLASERAGARKSMVG 300

Query: 363 SGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIA 419
           SGDRSERIRTYNFPQGRVTDHR+ +T H + E+++G  LD  V AL+ + E + +A
Sbjct: 301 SGDRSERIRTYNFPQGRVTDHRINLTLHRLSEILEGPGLDDLVGALIAEDEAERLA 356

BLAST of MC09g1914 vs. ExPASy Swiss-Prot
Match: A7HTY3 (Peptide chain release factor 1 OS=Parvibaculum lavamentivorans (strain DS-1 / DSM 13023 / NCIMB 13966) OX=402881 GN=prfA PE=3 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 7.2e-93
Identity = 175/354 (49.44%), Postives = 252/354 (71.19%), Query Frame = 0

Query: 66  IMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQKEINGL 125
           I E RL+++  R   + + +N  + P   +   +KE  +L      I  L +R++E    
Sbjct: 2   IPEERLQSVIARFEAVQSEMN-SDVPRDRFVALSKEFSELEPVAAAIGHLLARRREYADA 61

Query: 126 RTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVRAGTGG 185
             L+      +DM +MA EE+ +  EE  R+++ +   LLPKD AD+R+ ILEVRAGTGG
Sbjct: 62  EQLL----RVRDMAEMAKEEIARLNEELPRLEHEVQLMLLPKDAADDRNVILEVRAGTGG 121

Query: 186 EEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLKFESGI 245
           +EA+LFA D+F+MYERY   +GWK E++  +E E+ GYKE +A ISG G Y KLKFESG+
Sbjct: 122 DEAALFAGDLFRMYERYASNQGWKVELIAASEGEMGGYKEIIAGISGPGVYAKLKFESGV 181

Query: 246 HRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHANTTNS 305
           HRVQRVPVTE  GR+HTSA +VA+LP+A+EVDVE++++DLRID +R+ G+GGQH N T S
Sbjct: 182 HRVQRVPVTEGGGRIHTSAATVAVLPEAEEVDVEIEDKDLRIDVYRASGAGGQHVNKTES 241

Query: 306 AVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQIGSGD 365
           AVR+TH+PTG+ V+ QDE+SQH NKA+A++++ A++++ ER R+   RS  R  Q+GSGD
Sbjct: 242 AVRITHLPTGIVVAQQDEKSQHKNKARAMQILRARIFDAERERLDRERSAARKGQVGSGD 301

Query: 366 RSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIAS 420
           RSERIRTYNFPQ RVTDHR+ +T H +++V++G+ L   +DAL+ + +   +A+
Sbjct: 302 RSERIRTYNFPQSRVTDHRINLTLHKLDQVLEGEALGELIDALIAEDQAARLAA 350

BLAST of MC09g1914 vs. ExPASy Swiss-Prot
Match: A8GXI1 (Peptide chain release factor 1 OS=Rickettsia bellii (strain OSU 85-389) OX=391896 GN=prfA PE=3 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.6e-92
Identity = 169/320 (52.81%), Postives = 233/320 (72.81%), Query Frame = 0

Query: 94  EYAKANKELRKLRDTMDLINELRSRQKEINGLRTLMTECPEDKDMLDMATEELDQAVEEE 153
           E+ KA+KE   L D +  I E    + E+        E   DK  L+M  EE+    +  
Sbjct: 32  EFVKASKEYADLEDIVQKIKEYNKAKAELEEANNFKQEPSLDKATLEMIEEEIRNLEDSL 91

Query: 154 KRVQNLLLKSLLPKDDADERDCILEVRAGTGGEEASLFAMDIFKMYERYTQRKGWKFEVV 213
             ++  +  +LLPKD+AD +  I+E+RAGTGGEEA+LFA  +F MY+RY + KGW+FE++
Sbjct: 92  PTLERSVKIALLPKDEADSKSAIIEIRAGTGGEEAALFAAKLFNMYQRYAELKGWRFEIL 151

Query: 214 DITESELKGYKEAVAAISGAGAYGKLKFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQA 273
            I E+ + GYKE  A+I G   + KLKFESG+HRVQRVP TE  GR+HTSA +VA+LP+ 
Sbjct: 152 SIDETGIGGYKEVSASIKGKDVFSKLKFESGVHRVQRVPETESQGRIHTSAATVAVLPEV 211

Query: 274 DEVDVELKNEDLRIDTFRSGGSGGQHANTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKA 333
           ++VD++L+ +DLRIDT+R+ G+GGQH NTT+SAVR+THIPTG++V++QDE+SQH NKAKA
Sbjct: 212 EDVDIKLEEKDLRIDTYRASGAGGQHVNTTDSAVRITHIPTGITVALQDEKSQHKNKAKA 271

Query: 334 LKVICAKLYEKERCRIQSSRSKLRSEQIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIE 393
           LK++ A++YE+ER +    R+  R EQIGSGDRSERIRTYNFPQGRV+DHR+ +T + I+
Sbjct: 272 LKILRARIYEEERRKKDQERANNRREQIGSGDRSERIRTYNFPQGRVSDHRINLTLYKID 331

Query: 394 EVMQGQNLDMFVDALLLQQE 414
           EV+ GQ LD F++AL+   E
Sbjct: 332 EVINGQ-LDEFIEALIANDE 350

BLAST of MC09g1914 vs. ExPASy Swiss-Prot
Match: A8F1S1 (Peptide chain release factor 1 OS=Rickettsia massiliae (strain Mtu5) OX=416276 GN=prfA PE=3 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 2.1e-92
Identity = 166/320 (51.88%), Postives = 236/320 (73.75%), Query Frame = 0

Query: 94  EYAKANKELRKLRDTMDLINELRSRQKEINGLRTLMTECPEDKDMLDMATEELDQAVEEE 153
           E+ KA+KE  +L D +  I E    + E+        E   D   L+M  +E+       
Sbjct: 29  EFVKASKEYAELEDVVAKIKEYNKAKSELEEANNFKLEVGLDNATLEMIEDEIHTLENSL 88

Query: 154 KRVQNLLLKSLLPKDDADERDCILEVRAGTGGEEASLFAMDIFKMYERYTQRKGWKFEVV 213
            +++  +  +LLPKDDAD +  I+EVRAG+GGEEA+LFA  +F MY+RY + KGW+FE++
Sbjct: 89  PKLERAVKIALLPKDDADSKSAIIEVRAGSGGEEAALFAAVLFNMYQRYAELKGWRFEIL 148

Query: 214 DITESELKGYKEAVAAISGAGAYGKLKFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQA 273
            I+++ + GYKEA A+I G   + KLKFESG+HRVQRVP TE  GR+HTSA +VA+LP+A
Sbjct: 149 AISDTGIGGYKEASASIKGKDVFSKLKFESGVHRVQRVPETESQGRIHTSAATVAVLPEA 208

Query: 274 DEVDVELKNEDLRIDTFRSGGSGGQHANTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKA 333
           +EVD++++++DLRIDT+R+ G+GGQH NTT+SAVR+THIPTG++V++QDE+SQH NKAKA
Sbjct: 209 EEVDIQIEDKDLRIDTYRASGAGGQHVNTTDSAVRITHIPTGITVALQDEKSQHKNKAKA 268

Query: 334 LKVICAKLYEKERCRIQSSRSKLRSEQIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIE 393
           LK++ A++YE+ER + +  R+  R  Q+GSGDRSERIRTYNFPQGRV+DHR+ +T + I+
Sbjct: 269 LKILRARIYEEERRKKEQERADSRRGQVGSGDRSERIRTYNFPQGRVSDHRINLTLYKID 328

Query: 394 EVMQGQNLDMFVDALLLQQE 414
           EV++   LD FV+AL+   E
Sbjct: 329 EVVKNGQLDEFVEALIADDE 348

BLAST of MC09g1914 vs. NCBI nr
Match: XP_022151971.1 (uncharacterized protein LOC111019805 isoform X2 [Momordica charantia])

HSP 1 Score: 823 bits (2125), Expect = 2.70e-300
Identity = 424/424 (100.00%), Postives = 424/424 (100.00%), Query Frame = 0

Query: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKGEIQPQQLS 60
           MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKGEIQPQQLS
Sbjct: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKGEIQPQQLS 60

Query: 61  MELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQK 120
           MELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQK
Sbjct: 61  MELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQK 120

Query: 121 EINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVR 180
           EINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVR
Sbjct: 121 EINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLK 240
           AGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLK
Sbjct: 181 AGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQ 360
           NTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420

Query: 421 TSTS 424
           TSTS
Sbjct: 421 TSTS 424

BLAST of MC09g1914 vs. NCBI nr
Match: XP_022151970.1 (uncharacterized protein LOC111019805 isoform X1 [Momordica charantia])

HSP 1 Score: 818 bits (2113), Expect = 1.89e-298
Identity = 424/425 (99.76%), Postives = 424/425 (99.76%), Query Frame = 0

Query: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSK-GEIQPQQL 60
           MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSK GEIQPQQL
Sbjct: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKAGEIQPQQL 60

Query: 61  SMELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQ 120
           SMELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQ
Sbjct: 61  SMELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQ 120

Query: 121 KEINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEV 180
           KEINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEV
Sbjct: 121 KEINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEV 180

Query: 181 RAGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKL 240
           RAGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKL
Sbjct: 181 RAGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKL 240

Query: 241 KFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQH 300
           KFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQH
Sbjct: 241 KFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQH 300

Query: 301 ANTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSE 360
           ANTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSE
Sbjct: 301 ANTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSE 360

Query: 361 QIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIAS 420
           QIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIAS
Sbjct: 361 QIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIAS 420

Query: 421 FTSTS 424
           FTSTS
Sbjct: 421 FTSTS 425

BLAST of MC09g1914 vs. NCBI nr
Match: XP_022997983.1 (uncharacterized protein LOC111492771 [Cucurbita maxima])

HSP 1 Score: 735 bits (1898), Expect = 1.09e-265
Identity = 385/424 (90.80%), Postives = 400/424 (94.34%), Query Frame = 0

Query: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKGEIQPQQLS 60
           MRI+R T+RL+EQIRTLA H RTNP Q   NP LPPP   LSTP+ RFYSKGE+Q QQLS
Sbjct: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTPSVRFYSKGEVQTQQLS 60

Query: 61  MELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQK 120
           MELI IME RL+AIELRSTFLLNIINRPEAPP EYAKANKEL+KLRDTMDLINELRSRQK
Sbjct: 61  MELIKIMEQRLRAIELRSTFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120

Query: 121 EINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVR 180
           EINGLRTLM ECPEDKDMLDMATEEL+QAVEEEKR+Q+ LL SLLP+DDADER CILEVR
Sbjct: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLK 240
           AGTGGEEASLFAMDIFKMYERY Q+KGWKFEVVDITES+LKGYKEAVAAISGAG YGKLK
Sbjct: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQ 360
           NTTNSAVRVTHIPTGL+VSIQDERSQHMNKAKALKVICAKLYE ER RIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420

Query: 421 TSTS 424
           TS+S
Sbjct: 421 TSSS 424

BLAST of MC09g1914 vs. NCBI nr
Match: XP_022957138.1 (uncharacterized protein LOC111458610 [Cucurbita moschata] >KAG6601633.1 hypothetical protein SDJN03_06866, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032393.1 prfA [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 734 bits (1896), Expect = 2.20e-265
Identity = 384/424 (90.57%), Postives = 400/424 (94.34%), Query Frame = 0

Query: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKGEIQPQQLS 60
           MRI+R T+RL+EQIRTLA H RTNP Q   NP LPPP   LSTP+ RFYSKGE+Q QQLS
Sbjct: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQTCTNPLLPPPSDLLSTPSVRFYSKGEVQSQQLS 60

Query: 61  MELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQK 120
           MELI IME RLKAIELRS+FLLNIINRPEAPP EYAKANKEL+KLRDTMDLINELRSRQK
Sbjct: 61  MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120

Query: 121 EINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVR 180
           EINGLRTLM ECPEDKDMLDMATEEL+QAVEEEKR+Q+ LL SLLP+DDADER CILEVR
Sbjct: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLK 240
           AGTGGEEASLFAMDIFKMYERY Q+KGWKFEVVDITES+LKGYKEAVAAISGAG YGKLK
Sbjct: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQ 360
           NTTNSAVRVTHIP+GL+VSIQDERSQHMNKAKALKVICAKLYE ER RIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVTHIPSGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420

Query: 421 TSTS 424
           TS+S
Sbjct: 421 TSSS 424

BLAST of MC09g1914 vs. NCBI nr
Match: XP_023527687.1 (uncharacterized protein LOC111790812 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 734 bits (1894), Expect = 4.43e-265
Identity = 385/424 (90.80%), Postives = 399/424 (94.10%), Query Frame = 0

Query: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKGEIQPQQLS 60
           MRI+R T+RL+EQIRTLA H RTNP Q   NP LPPP   LST  FRFYSKGE+Q QQLS
Sbjct: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTSPFRFYSKGEVQSQQLS 60

Query: 61  MELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQK 120
           MELI IME RLKAIELRS+FLLNIINRPEAPP EYAKANKEL+KLRDTMDLINELRSRQK
Sbjct: 61  MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120

Query: 121 EINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVR 180
           EINGLRTLM ECPEDKDMLDMATEEL+QAVEEEKR+Q+ LL SLLP+DDADER CILEVR
Sbjct: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLK 240
           AGTGGEEASLFAMDIFKMYERY Q+KGWKFEVVDITES+LKGYKEAVAAISGAG YGKLK
Sbjct: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQ 360
           NTTNSAVRVTHIPTGL+VSIQDERSQHMNKAKALKVICAKLYE ER RIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420

Query: 421 TSTS 424
           TS+S
Sbjct: 421 TSSS 424

BLAST of MC09g1914 vs. ExPASy TrEMBL
Match: A0A6J1DG80 (uncharacterized protein LOC111019805 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019805 PE=3 SV=1)

HSP 1 Score: 823 bits (2125), Expect = 1.31e-300
Identity = 424/424 (100.00%), Postives = 424/424 (100.00%), Query Frame = 0

Query: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKGEIQPQQLS 60
           MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKGEIQPQQLS
Sbjct: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKGEIQPQQLS 60

Query: 61  MELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQK 120
           MELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQK
Sbjct: 61  MELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQK 120

Query: 121 EINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVR 180
           EINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVR
Sbjct: 121 EINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLK 240
           AGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLK
Sbjct: 181 AGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQ 360
           NTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420

Query: 421 TSTS 424
           TSTS
Sbjct: 421 TSTS 424

BLAST of MC09g1914 vs. ExPASy TrEMBL
Match: A0A6J1DCN5 (uncharacterized protein LOC111019805 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019805 PE=3 SV=1)

HSP 1 Score: 818 bits (2113), Expect = 9.17e-299
Identity = 424/425 (99.76%), Postives = 424/425 (99.76%), Query Frame = 0

Query: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSK-GEIQPQQL 60
           MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSK GEIQPQQL
Sbjct: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKAGEIQPQQL 60

Query: 61  SMELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQ 120
           SMELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQ
Sbjct: 61  SMELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQ 120

Query: 121 KEINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEV 180
           KEINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEV
Sbjct: 121 KEINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEV 180

Query: 181 RAGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKL 240
           RAGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKL
Sbjct: 181 RAGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKL 240

Query: 241 KFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQH 300
           KFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQH
Sbjct: 241 KFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQH 300

Query: 301 ANTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSE 360
           ANTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSE
Sbjct: 301 ANTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSE 360

Query: 361 QIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIAS 420
           QIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIAS
Sbjct: 361 QIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIAS 420

Query: 421 FTSTS 424
           FTSTS
Sbjct: 421 FTSTS 425

BLAST of MC09g1914 vs. ExPASy TrEMBL
Match: A0A6J1KFI0 (uncharacterized protein LOC111492771 OS=Cucurbita maxima OX=3661 GN=LOC111492771 PE=3 SV=1)

HSP 1 Score: 735 bits (1898), Expect = 5.27e-266
Identity = 385/424 (90.80%), Postives = 400/424 (94.34%), Query Frame = 0

Query: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKGEIQPQQLS 60
           MRI+R T+RL+EQIRTLA H RTNP Q   NP LPPP   LSTP+ RFYSKGE+Q QQLS
Sbjct: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQICTNPLLPPPSDLLSTPSVRFYSKGEVQTQQLS 60

Query: 61  MELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQK 120
           MELI IME RL+AIELRSTFLLNIINRPEAPP EYAKANKEL+KLRDTMDLINELRSRQK
Sbjct: 61  MELIKIMEQRLRAIELRSTFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120

Query: 121 EINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVR 180
           EINGLRTLM ECPEDKDMLDMATEEL+QAVEEEKR+Q+ LL SLLP+DDADER CILEVR
Sbjct: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLK 240
           AGTGGEEASLFAMDIFKMYERY Q+KGWKFEVVDITES+LKGYKEAVAAISGAG YGKLK
Sbjct: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQ 360
           NTTNSAVRVTHIPTGL+VSIQDERSQHMNKAKALKVICAKLYE ER RIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVTHIPTGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420

Query: 421 TSTS 424
           TS+S
Sbjct: 421 TSSS 424

BLAST of MC09g1914 vs. ExPASy TrEMBL
Match: A0A6J1GZD2 (uncharacterized protein LOC111458610 OS=Cucurbita moschata OX=3662 GN=LOC111458610 PE=3 SV=1)

HSP 1 Score: 734 bits (1896), Expect = 1.06e-265
Identity = 384/424 (90.57%), Postives = 400/424 (94.34%), Query Frame = 0

Query: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKGEIQPQQLS 60
           MRI+R T+RL+EQIRTLA H RTNP Q   NP LPPP   LSTP+ RFYSKGE+Q QQLS
Sbjct: 1   MRIHRPTVRLLEQIRTLALHCRTNPAQTCTNPLLPPPSDLLSTPSVRFYSKGEVQSQQLS 60

Query: 61  MELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQK 120
           MELI IME RLKAIELRS+FLLNIINRPEAPP EYAKANKEL+KLRDTMDLINELRSRQK
Sbjct: 61  MELIKIMEQRLKAIELRSSFLLNIINRPEAPPSEYAKANKELKKLRDTMDLINELRSRQK 120

Query: 121 EINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVR 180
           EINGLRTLM ECPEDKDMLDMATEEL+QAVEEEKR+Q+ LL SLLP+DDADER CILEVR
Sbjct: 121 EINGLRTLMDECPEDKDMLDMATEELNQAVEEEKRLQHSLLTSLLPRDDADERGCILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLK 240
           AGTGGEEASLFAMDIFKMYERY Q+KGWKFEVVDITES+LKGYKEAVAAISGAG YGKLK
Sbjct: 181 AGTGGEEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAVAAISGAGVYGKLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQ 360
           NTTNSAVRVTHIP+GL+VSIQDERSQHMNKAKALKVICAKLYE ER RIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVTHIPSGLTVSIQDERSQHMNKAKALKVICAKLYEIERRRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420

Query: 421 TSTS 424
           TS+S
Sbjct: 421 TSSS 424

BLAST of MC09g1914 vs. ExPASy TrEMBL
Match: A0A1S4DW68 (peptide chain release factor 1 OS=Cucumis melo OX=3656 GN=LOC103488835 PE=3 SV=1)

HSP 1 Score: 711 bits (1835), Expect = 2.08e-256
Identity = 367/424 (86.56%), Postives = 396/424 (93.40%), Query Frame = 0

Query: 1   MRINRGTLRLVEQIRTLAPHWRTNPVQNTPNPFLPPPFQPLSTPTFRFYSKGEIQPQQLS 60
           MRINRGT+RLVEQIRTLA H R N  + T NP LP  F PLS+P+FRFYSKGE+Q QQLS
Sbjct: 1   MRINRGTMRLVEQIRTLALHCRNNSSRITKNPSLPFHFDPLSSPSFRFYSKGEVQSQQLS 60

Query: 61  MELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANKELRKLRDTMDLINELRSRQK 120
           MELI IME RL AIELR+TFLLNIINRPEAPP +YAKANKEL+KLRDTM+LI ELRS+QK
Sbjct: 61  MELIKIMEQRLTAIELRNTFLLNIINRPEAPPADYAKANKELKKLRDTMNLITELRSKQK 120

Query: 121 EINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLLLKSLLPKDDADERDCILEVR 180
           EINGLRTLM EC ED+DM DMATEEL+QAVEEEKR+Q LLLKSLLP+DDADERD ILEVR
Sbjct: 121 EINGLRTLMEECSEDRDMFDMATEELEQAVEEEKRLQQLLLKSLLPRDDADERDSILEVR 180

Query: 181 AGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISGAGAYGKLK 240
           AGTGG+EASLFAMDIFKMYERY Q+KGWKFEVVDITES+LKGYKEA+AAISGAG YG+LK
Sbjct: 181 AGTGGDEASLFAMDIFKMYERYAQKKGWKFEVVDITESDLKGYKEAIAAISGAGVYGRLK 240

Query: 241 FESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVELKNEDLRIDTFRSGGSGGQHA 300
           FESGIHRVQRVPVTEKSGRVHTSA+SVAILPQADEVDVELKNEDLRIDT+RSGGSGGQHA
Sbjct: 241 FESGIHRVQRVPVTEKSGRVHTSAISVAILPQADEVDVELKNEDLRIDTYRSGGSGGQHA 300

Query: 301 NTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQSSRSKLRSEQ 360
           NTTNSAVRV HIPTGL+V+IQDERSQHMNKA+ALK+ICAKLYE ER RIQSSRSKLRSEQ
Sbjct: 301 NTTNSAVRVIHIPTGLTVTIQDERSQHMNKARALKLICAKLYEMERRRIQSSRSKLRSEQ 360

Query: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALLLQQEMDAIASF 420
           IGSGDRSERIRTYNFPQGRVTDHRVGIT+H+IEEVMQG+NLD FVDALLLQQEMDAIASF
Sbjct: 361 IGSGDRSERIRTYNFPQGRVTDHRVGITDHSIEEVMQGENLDTFVDALLLQQEMDAIASF 420

Query: 421 TSTS 424
           TS+S
Sbjct: 421 TSSS 424

BLAST of MC09g1914 vs. TAIR 10
Match: AT2G47020.1 (Peptide chain release factor 1 )

HSP 1 Score: 581.3 bits (1497), Expect = 6.5e-166
Identity = 296/384 (77.08%), Postives = 349/384 (90.89%), Query Frame = 0

Query: 41  LSTPTFRFYSKGEIQPQQLSMELINIMEHRLKAIELRSTFLLNIINRPEAPPVEYAKANK 100
           LS+   R Y+ G ++P QLS +LI IM+ RL AIE R+  L  +IN+PE  P E+++ANK
Sbjct: 32  LSSNMIRLYTTG-MEP-QLSPDLIKIMDQRLSAIEHRNAVLQKLINQPEYSPEEFSRANK 91

Query: 101 ELRKLRDTMDLINELRSRQKEINGLRTLMTECPEDKDMLDMATEELDQAVEEEKRVQNLL 160
           ELRKLRD+M LIN+LR++QKEI+GL++L++E  +DKDMLD+A  ELD+AVEEEKR+Q LL
Sbjct: 92  ELRKLRDSMLLINDLRAKQKEIDGLKSLVSESSDDKDMLDLAVGELDEAVEEEKRLQTLL 151

Query: 161 LKSLLPKDDADERDCILEVRAGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESEL 220
           LKSLLPKD+ADERDCILEVRAGTGGEEASLFAMDIF+MYERY+Q+KGWKF++VDITES++
Sbjct: 152 LKSLLPKDEADERDCILEVRAGTGGEEASLFAMDIFRMYERYSQKKGWKFDIVDITESDM 211

Query: 221 KGYKEAVAAISGAGAYGKLKFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADEVDVEL 280
           KGYKEA AAI GA  YGKLKFESGIHRVQR+P+TEKSGR+HTSA+SVAILPQADEVDV+L
Sbjct: 212 KGYKEASAAICGASVYGKLKFESGIHRVQRIPITEKSGRIHTSAISVAILPQADEVDVQL 271

Query: 281 KNEDLRIDTFRSGGSGGQHANTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAK 340
           +NEDLRIDT+RSGGSGGQHANTTNSAVR+ H+PTG+ VSIQDERSQHMN+AKALKV+CA+
Sbjct: 272 RNEDLRIDTYRSGGSGGQHANTTNSAVRIIHLPTGMMVSIQDERSQHMNRAKALKVLCAR 331

Query: 341 LYEKERCRIQSSRSKLRSEQIGSGDRSERIRTYNFPQGRVTDHRVGITEHAIEEVMQGQN 400
           LYE ER RIQSSRSKLRS+QIGSGDRS RIRTYNFPQGRVTDHRVGIT HAIE++MQG+N
Sbjct: 332 LYEIERLRIQSSRSKLRSDQIGSGDRSGRIRTYNFPQGRVTDHRVGITHHAIEDMMQGEN 391

Query: 401 LDMFVDALLLQQEMDAIASFTSTS 425
           LDMF+DALLL+QEMDAIASF+STS
Sbjct: 392 LDMFIDALLLRQEMDAIASFSSTS 413

BLAST of MC09g1914 vs. TAIR 10
Match: AT2G47020.2 (Peptide chain release factor 1 )

HSP 1 Score: 394.4 bits (1012), Expect = 1.1e-109
Identity = 192/245 (78.37%), Postives = 230/245 (93.88%), Query Frame = 0

Query: 87  RPEAPPVEYAKANKELRKLRDTMDLINELRSRQKEINGLRTLMTECPEDKDMLDMATEEL 146
           +PE  P E+++ANKELRKLRD+M LIN+LR++QKEI+GL++L++E  +DKDMLD+A  EL
Sbjct: 11  QPEYSPEEFSRANKELRKLRDSMLLINDLRAKQKEIDGLKSLVSESSDDKDMLDLAVGEL 70

Query: 147 DQAVEEEKRVQNLLLKSLLPKDDADERDCILEVRAGTGGEEASLFAMDIFKMYERYTQRK 206
           D+AVEEEKR+Q LLLKSLLPKD+ADERDCILEVRAGTGGEEASLFAMDIF+MYERY+Q+K
Sbjct: 71  DEAVEEEKRLQTLLLKSLLPKDEADERDCILEVRAGTGGEEASLFAMDIFRMYERYSQKK 130

Query: 207 GWKFEVVDITESELKGYKEAVAAISGAGAYGKLKFESGIHRVQRVPVTEKSGRVHTSAVS 266
           GWKF++VDITES++KGYKEA AAI GA  YGKLKFESGIHRVQR+P+TEKSGR+HTSA+S
Sbjct: 131 GWKFDIVDITESDMKGYKEASAAICGASVYGKLKFESGIHRVQRIPITEKSGRIHTSAIS 190

Query: 267 VAILPQADEVDVELKNEDLRIDTFRSGGSGGQHANTTNSAVRVTHIPTGLSVSIQDERSQ 326
           VAILPQADEVDV+L+NEDLRIDT+RSGGSGGQHANTTNSAVR+ H+PTG+ VSIQDERSQ
Sbjct: 191 VAILPQADEVDVQLRNEDLRIDTYRSGGSGGQHANTTNSAVRIIHLPTGMMVSIQDERSQ 250

Query: 327 HMNKA 332
           HM+++
Sbjct: 251 HMSQS 255

BLAST of MC09g1914 vs. TAIR 10
Match: AT3G62910.1 (Peptide chain release factor 1 )

HSP 1 Score: 245.4 bits (625), Expect = 8.5e-65
Identity = 132/341 (38.71%), Postives = 216/341 (63.34%), Query Frame = 0

Query: 94  EYAKANKELRKLRDTMDLINELRSRQKEINGLRTLMTECPEDKDMLDMATEELDQAVEEE 153
           EY K  + + +L + + +    +  +K++   + L  E  +D+DM +M   E++   +E 
Sbjct: 86  EYQKLAQSMSELDEVVTVFRRFKDCEKQLLESKVLAKEAGDDEDMAEMIGSEINSLTKEI 145

Query: 154 KRVQNLLLKSLLPKDDADERDCILEVRAGTGGEEASLFAMDIFKMYERYTQRKGWKFEVV 213
           + ++  L   LLP D  D R+ +LEVRAGTGG+EA+++  D+ +MY+RY++R  WKF +V
Sbjct: 146 EELEKQLKVLLLPSDPLDARNILLEVRAGTGGDEAAIWTGDLVRMYQRYSERSSWKFSMV 205

Query: 214 DITESELKGYKEAVAAISGAGAYGKLKFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQA 273
             +E+E  GYK  V  I G   Y KLK+ESG+HRVQRVP TE  GRVHTS  +VAI+P+A
Sbjct: 206 SCSEAEHGGYKTCVMEIKGNRVYSKLKYESGVHRVQRVPQTETQGRVHTSTATVAIMPEA 265

Query: 274 DEVDVELKNEDLRIDTFRSGGSGGQHANTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKA 333
           DEV+V +  +D+ + + RSGG+GGQ+ N   +A+ + H P+G+ +   +ER+Q  NKA+A
Sbjct: 266 DEVEVVIDPKDIELTSARSGGAGGQNVNKVETAIDLFHKPSGIRIFCTEERTQIRNKARA 325

Query: 334 LKVICAKLYEKERCRIQSSRSKLRSE---QIGSGDRSERIRTYNFPQGRVTDHRVGIT-- 393
            +++ AKLYE    +++  + K+R+E   Q+G+G RSE+IRTYN+   RVTDHR+ +   
Sbjct: 326 FQLLRAKLYE---IKVREQQEKIRNERKSQVGTGARSEKIRTYNYKDSRVTDHRLKMNFA 385

Query: 394 -----EHAIEEVMQGQNLDMFVDALLLQQEMDAIASFTSTS 425
                + A+E+ +Q           L+++  +++A+ ++TS
Sbjct: 386 LTTFLDGALEDAVQA--CAALEQKELMEELSESVAASSATS 421

BLAST of MC09g1914 vs. TAIR 10
Match: AT1G56350.1 (Peptide chain release factor 2 )

HSP 1 Score: 171.8 bits (434), Expect = 1.2e-42
Identity = 112/300 (37.33%), Postives = 168/300 (56.00%), Query Frame = 0

Query: 121 EINGLRTLMTECPEDKDMLDMATEELDQAVEEE--------KRVQNLLLKSLLPKDDADE 180
           ++ G+ T   E  E  DML +A EE D  +E E        +RV        L   D D 
Sbjct: 174 KMKGVMTFERELLEHIDMLKLAKEENDSELESETLKALIDMRRVSKEKELEALLSADNDP 233

Query: 181 RDCILEVRAGTGGEEASLFAMDIFKMYERYTQRKGWKFEVVDITESELKGYKEAVAAISG 240
             C +EV+AG GG E++ +A  + +MY+ + QR+ +   VVD    E+ G K A   ++G
Sbjct: 234 CSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSVTVVDEAPGEIAGIKRATIKVNG 293

Query: 241 AGAYGKLKFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQADE--VDVELKNEDLRIDTF 300
             AYG  K E G+HR+ R+   +   R HTS  +VA++P   +    VE+ + DLRI+ F
Sbjct: 294 EYAYGYAKAEVGVHRLVRISPFDSGKRRHTSFAAVAVIPILGDGSTRVEINDSDLRIERF 353

Query: 301 RSGGSGGQHANTTNSAVRVTHIPTGLSVSIQDERSQHMNKAKALKVICAKLYEKERCRIQ 360
           RSGG+GGQHANTT+SAVR+ HIPTG++ + Q+ERSQH NKA A+ V+ ++L + E  R Q
Sbjct: 354 RSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHSNKASAMAVLQSRLDQLEMAR-Q 413

Query: 361 SSRSKLRSEQIGSGDRSERIRTYNF-PQGRVTDHRVGITEHAIEEVMQGQNLDMFVDALL 410
           ++ +   ++ +       +IRTY   P   V D R        + V++G +LD F+ + L
Sbjct: 414 TAMNAQHTQSLTEISWGNQIRTYVLHPYRMVKDLRTNYEVSDPDSVLEG-DLDGFILSFL 471

BLAST of MC09g1914 vs. TAIR 10
Match: AT5G36170.1 (high chlorophyll fluorescent 109 )

HSP 1 Score: 140.2 bits (352), Expect = 3.8e-33
Identity = 108/330 (32.73%), Postives = 170/330 (51.52%), Query Frame = 0

Query: 104 KLRDTMDLINELRSRQKEINGLRTLMTECPEDKDMLDMATEELDQA----VEEEKRVQNL 163
           K ++T+  +N+L+ R + ++  +T++    ED + +   TEE+D      +EE   +   
Sbjct: 129 KAQETLSSLNDLKDRMRLLSEFKTMV----EDAETIVKLTEEMDSTDVSLLEEAMGIIKE 188

Query: 164 LLKSL-------LPKDDADERDCILEVRAGTGGEEASLFAMDIFKMYERYTQRKGWKFEV 223
           L KSL       L     D+   ++ + AG GG +A  +A  + +MY R+ +++ +K +V
Sbjct: 189 LNKSLDKFELTQLLSGPYDKEGAVVYITAGAGGTDAQDWADMLLRMYMRWGEKQRYKTKV 248

Query: 224 VDITESELKGYKEAVAAISGAGAYGKLKFESGIHRVQRVPVTEKSGRVHTSAVSVAILPQ 283
           V+++  E  G K A   I G  AYG +  E G HR+ R       G   TS   V ++P 
Sbjct: 249 VEMSNGEEAGIKSATLEIEGRYAYGYISGEKGTHRIVRQSPFNSKGLRQTSFSGVEVMPL 308

Query: 284 ADE--VDVELKNEDLRIDTFRSGGSGGQHANTTNSAVRVTHIPTGLSVSIQDERSQHMNK 343
             E  V +E+  EDL I   R+GG GGQ+ N   +AVR+THIPTG++V   +ERSQ  NK
Sbjct: 309 LPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANK 368

Query: 344 AKALKVICAKLYEKERCRIQSSRSKLRSEQIGSGDRSERIRTYNF-PQGRVTDHRVGITE 403
            +AL  + AKL      +  +   ++R + +   +  ++IR Y F P   V D R G   
Sbjct: 369 TRALIRLKAKLMVIAEEQRATEIKEIRGDAV-KAEWGQQIRNYVFHPYKLVKDVRTGHET 428

Query: 404 HAIEEVMQGQNLDMFVDALLLQQEMDAIAS 420
             I  VM G +LD F+ A L  +   A+AS
Sbjct: 429 SDITSVMDG-DLDPFIKAYLKHKYTLAMAS 452

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A2RVR79.2e-16577.08Peptide chain release factor 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN... [more]
A5V9C34.2e-9348.31Peptide chain release factor 1 OS=Sphingomonas wittichii (strain RW1 / DSM 6014 ... [more]
A7HTY37.2e-9349.44Peptide chain release factor 1 OS=Parvibaculum lavamentivorans (strain DS-1 / DS... [more]
A8GXI11.6e-9252.81Peptide chain release factor 1 OS=Rickettsia bellii (strain OSU 85-389) OX=39189... [more]
A8F1S12.1e-9251.88Peptide chain release factor 1 OS=Rickettsia massiliae (strain Mtu5) OX=416276 G... [more]
Match NameE-valueIdentityDescription
XP_022151971.12.70e-300100.00uncharacterized protein LOC111019805 isoform X2 [Momordica charantia][more]
XP_022151970.11.89e-29899.76uncharacterized protein LOC111019805 isoform X1 [Momordica charantia][more]
XP_022997983.11.09e-26590.80uncharacterized protein LOC111492771 [Cucurbita maxima][more]
XP_022957138.12.20e-26590.57uncharacterized protein LOC111458610 [Cucurbita moschata] >KAG6601633.1 hypothet... [more]
XP_023527687.14.43e-26590.80uncharacterized protein LOC111790812 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1DG801.31e-300100.00uncharacterized protein LOC111019805 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1DCN59.17e-29999.76uncharacterized protein LOC111019805 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1KFI05.27e-26690.80uncharacterized protein LOC111492771 OS=Cucurbita maxima OX=3661 GN=LOC111492771... [more]
A0A6J1GZD21.06e-26590.57uncharacterized protein LOC111458610 OS=Cucurbita moschata OX=3662 GN=LOC1114586... [more]
A0A1S4DW682.08e-25686.56peptide chain release factor 1 OS=Cucumis melo OX=3656 GN=LOC103488835 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G47020.16.5e-16677.08Peptide chain release factor 1 [more]
AT2G47020.21.1e-10978.37Peptide chain release factor 1 [more]
AT3G62910.18.5e-6538.71Peptide chain release factor 1 [more]
AT1G56350.11.2e-4237.33Peptide chain release factor 2 [more]
AT5G36170.13.8e-3332.73high chlorophyll fluorescent 109 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 95..115
NoneNo IPR availableCOILSCoilCoilcoord: 139..159
NoneNo IPR availableGENE3D3.30.70.1660coord: 177..405
e-value: 9.2E-86
score: 288.4
NoneNo IPR availableGENE3D3.30.160.20coord: 273..368
e-value: 9.2E-86
score: 288.4
NoneNo IPR availableGENE3D6.10.140.1950coord: 59..169
e-value: 5.0E-15
score: 57.6
NoneNo IPR availablePANTHERPTHR43804:SF7LD18447Pcoord: 53..421
NoneNo IPR availablePANTHERPTHR43804LD18447Pcoord: 53..421
NoneNo IPR availableSUPERFAMILY75620Release factorcoord: 93..412
IPR005139Peptide chain release factorSMARTSM00937PCRF_a_2coord: 127..242
e-value: 2.3E-52
score: 189.9
IPR005139Peptide chain release factorPFAMPF03462PCRFcoord: 93..267
e-value: 5.0E-61
score: 205.9
IPR004373Peptide chain release factor 1TIGRFAMTIGR00019TIGR00019coord: 84..420
e-value: 2.1E-130
score: 432.8
IPR004373Peptide chain release factor 1HAMAPMF_00093Rel_fac_1coord: 67..421
score: 34.082581
IPR000352Peptide chain release factor class IPFAMPF00472RF-1coord: 275..384
e-value: 3.6E-36
score: 123.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g1914.1MC09g1914.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006415 translational termination
molecular_function GO:0016149 translation release factor activity, codon specific
molecular_function GO:0003747 translation release factor activity