MC09g1852 (gene) Bitter gourd (Dali-11) v1

BLAST of MC09g1852 vs. ExPASy Swiss-Prot
Match: P42534 (Putative polyketide hydroxylase OS=Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) OX=100226 GN=SCO5321 PE=3 SV=2)

HSP 1 Score: 199.9 bits (507), Expect = 9.8e-50
Identity = 200/679 (29.46%), Postives = 292/679 (43.00%), Query Frame = 0

Query: 61  GGEDT-MVPVLIVGAGPVGLVLAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFR 120
           GG+ T  VPVL+VG   VGL  ++ L +LGV+  +VE++   S HP+    N R+ME+FR
Sbjct: 14  GGDRTHRVPVLVVGGSLVGLSTSVFLGRLGVRHTLVERHAGTSIHPRGRGNNVRTMEIFR 73

Query: 121 KLHGLAEEIQLCQPPVDSWRKFIYCTSLNGAILGSVDHMQPQDFGNI--ISPVSVAHFSQ 180
            + G   +I+     +      +   +L G   G     Q    G +   SP S    SQ
Sbjct: 74  -VAGTEPDIRRAAATLADNHGILQAPTLAGD-AGEWLFKQIDPGGGLARFSPSSWCLCSQ 133

Query: 181 YKLNRLLLKKLQNLGFQVCSPDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKE 240
             L   LL    NLG                   +  G E +S +A  + VT      + 
Sbjct: 134 NDLEPELLTHATNLG-----------------GDLRFGTELLSFEADTEGVTAIVKSRET 193

Query: 241 GKHTERRNICSNILVGTDGAGSTVRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERP 300
           G+HT    I ++ LV  DG  S VR  +GI   G  DL   VS+ F SR L + ++ +R 
Sbjct: 194 GEHT---TIRADYLVAADGPRSPVREQLGIGQSGPGDLFHNVSITFRSRRLAD-VVGDRR 253

Query: 301 GMLYFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQ-QSIEDFSPKMCEELIFKLVGLNLC 360
            ++ ++ +  A G L+  D ++  ++   P++P Q +++EDF+ + C   I + +G    
Sbjct: 254 FIVCYLTDENADGALLPVDNRE-NWVFHAPWHPEQGETVEDFTDERCAAHIRRAIGDPDL 313

Query: 361 DIDVQDVKPWIMHAEVAEKFICCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLA 420
           D+++    PW     VA  +     RVLLAGD+AH   P G FG NTGIQD HNLAWKLA
Sbjct: 314 DVEITGKAPWHAAQRVARSY--RSGRVLLAGDSAHEMSPTGAFGSNTGIQDAHNLAWKLA 373

Query: 421 AVLQDIASPSILNTYEMERRPIAQFNTA----LSVKNFKAAMEVPAALGLDPRIANSVHQ 480
           AVL+  A  ++L+TY+ ERRP+A+  +A     SV++       P   G     A +   
Sbjct: 374 AVLEGWAGEALLDTYDTERRPVAEATSARAAHRSVEHSHPGFAPPPVAGGGGPGAGTPGG 433

Query: 481 AVNHGLGSVLPSSLQSSVLDGIFKIGRMQLSESLLNDKNPIGSSRLAKLRQIFDE----- 540
           A   G G+  P         G   +G             P G       R          
Sbjct: 434 A---GRGTGGPGG-----PGGPGGLGGPGGPGGTGGPGGPGGPGGPDGPRGAGGAPGGGP 493

Query: 541 -----GKSLQLQFPAEDLGFRYSEGALIPDNTLLSGPEEPTGRRRQYVPSADPGSRLPHM 600
                G   Q       LG+RY  GA++  +   + P  P G       +  PGSR PH+
Sbjct: 494 GGGPGGGGPQRGILNVALGYRYPRGAVVGADP--ATPVVPEGLDL----TGAPGSRAPHL 553

Query: 601 NVRTLASEDMISTLDLVSGDKVEFLLIIAPLPESYHLARSALKIAAEEFKTSVKVCVLWS 660
            VR    +D +STLDL     V  LL  A  P  +H A + +         S +V     
Sbjct: 554 WVR--RGQDRLSTLDLYEDSLV--LLSDAAQPTGWHEAAAGVAAGMRVPLKSYRV----- 613

Query: 661 ADIPRVESRSQQELTPWENYMDVQEIRQSSTSPSWWDVCRMTDNGAILVRPDEHIAWRVK 720
              P  +     E T W     V                  T  GA+LVRPD  +AWR  
Sbjct: 614 GGSPGADLNPDDEETDWARAHGV------------------TRGGAVLVRPDGFVAWR-S 624

Query: 721 SGVSGDLNTQMKRVFTALL 722
            G + D  + +++V   +L
Sbjct: 674 PGPAPDPESMLRQVVGTVL 624

BLAST of MC09g1852 vs. ExPASy Swiss-Prot
Match: Q05355 (Putative polyketide hydroxylase OS=Streptomyces halstedii OX=1944 GN=schC PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.7e-49
Identity = 198/666 (29.73%), Postives = 284/666 (42.64%), Query Frame = 0

Query: 67  VPVLIVGAGPVGLVLAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRKLHGLAE 126
           VPVL+VG   VGL  ++ L +LGV+  +VE++   S HP+    N R+MEV+R   G+ +
Sbjct: 15  VPVLVVGGSLVGLSTSVFLGRLGVRHMLVERHAGTSVHPRGRGNNVRTMEVYRAA-GVEQ 74

Query: 127 EIQLCQPPVDSWRKFIYCTSLNGA----ILGSVDHMQPQDFGNIISPVSVAHFSQYKLNR 186
            I+     +      +   SL G     +L  +D   P       SP S    SQ  L  
Sbjct: 75  GIRRAAATLAGNHGILQTPSLVGDEGEWLLRDID---PGGGLARFSPSSWCLCSQNDLEP 134

Query: 187 LLLKKLQNLGFQVCSPDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKHTE 246
           +LL     LG                  +I    E  S +     VT      + G+HT 
Sbjct: 135 VLLDHAVELG-----------------GEIRFSTELQSFEQDPAGVTAVIKSRRSGEHTT 194

Query: 247 RRNICSNILVGTDGAGSTVRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGMLYF 306
            R   ++ LV  DG  S VR  +GI   G  DL   VSV F SR L E+ + +R  ++ +
Sbjct: 195 VR---ADYLVAADGPRSPVREQLGIGQSGPGDLFHNVSVTFRSRRLAEF-VGDRHFIVCY 254

Query: 307 IFNTEAIGVLVAHDLKQGEFILQVPFYP-PQQSIEDFSPKMCEELIFKLVGLNLCDIDVQ 366
           + N EA G L+  D ++  ++   P+YP   + +EDF+ + C + I + VG+   D+++ 
Sbjct: 255 LTNPEADGALLPVDNRE-NWVFHAPWYPRAARPLEDFTDERCADHIRRAVGVPDLDVEIT 314

Query: 367 DVKPWIMHAEVAEKFICCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQD 426
              PW     VA ++     RV LAGD+AH   P G FG NTGIQD HNLAWKLAAVL  
Sbjct: 315 GKAPWHAAQRVARQYRA--GRVFLAGDSAHEMSPTGAFGSNTGIQDAHNLAWKLAAVLGG 374

Query: 427 IASPSILNTYEMERRPIAQFNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGSV 486
            A   +L+TY+ ERRP+A+  TA      +AA           R A   H       G  
Sbjct: 375 WAGDGLLDTYDAERRPVAEATTA------RAA----------ARSAEHSHP------GFA 434

Query: 487 LPSSLQSSVLDGIFKIGRMQLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDLG 546
            P         GI  +                                          LG
Sbjct: 435 PPPGTSGGPQGGILNVA-----------------------------------------LG 494

Query: 547 FRYSEGALIPDNTLLSGPEEPTGRRRQYVPSA-----DPGSRLPHMNVRTLASEDMISTL 606
           +RY  GA+     L + P  P       VP A     +PGSR PH+ +      + +STL
Sbjct: 495 YRYPGGAV-----LGADPATPV------VPEALTLAGEPGSRAPHLWMSRRG--ERLSTL 552

Query: 607 DLVSGDKVEFLLIIAPLPESYHLARSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQEL 666
           DL     V      A  P+++H   SA+++A E    SV +         RV   +  +L
Sbjct: 555 DLYERSPVLLSDADAGAPDAWH--ESAVRLAEE---LSVPLTSY------RVGRSAGADL 552

Query: 667 TPWENYMDVQEIRQSSTSPSWWDVCRMTDNGAILVRPDEHIAWRVKSGV-SGDLNTQMKR 722
           TP +   DV    +  T P           GA+LVRPD  +AWR +  V + +    ++ 
Sbjct: 615 TPED---DVNWTARHGTPP----------GGAVLVRPDGFVAWRSQEPVPAEETEPTLRH 552

BLAST of MC09g1852 vs. ExPASy Swiss-Prot
Match: Q8KN28 (2,4-dichlorophenol 6-monooxygenase OS=Delftia acidovorans OX=80866 GN=tfdB PE=1 SV=3)

HSP 1 Score: 191.0 bits (484), Expect = 4.5e-47
Identity = 179/664 (26.96%), Postives = 274/664 (41.27%), Query Frame = 0

Query: 69  VLIVGAGPVGLVLAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRKLHGLAEEI 128
           VL+VG+GP G    +LL   GVK   V K  + S  P++H  N R+MEV R L GL  E 
Sbjct: 12  VLVVGSGPAGAASTLLLATYGVKTLCVSKYATTSRTPRSHITNQRTMEVMRDL-GLELEC 71

Query: 129 QLCQPPVDSWRKFIYCTSLNGAILGSV----DHMQPQDFGNIISPVSVAHFSQYKLNRLL 188
           +    P +   + +YCTSL G  LG V     H Q +    + SP  +    Q  L  ++
Sbjct: 72  EAMASPAELMGENVYCTSLVGDELGRVLTWGTHPQRRADYELASPTHMCDLPQNLLEPIM 131

Query: 189 LKKLQNLGFQVCSPDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKHTERR 248
           +      G                   +    E VS+   +  VT T   +++     + 
Sbjct: 132 INHAARRG-----------------ADVRFHTEFVSLKQDETGVTAT---VRDHLLDRQY 191

Query: 249 NICSNILVGTDGAGSTVRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGMLYFIF 308
           +I +  L+G DGA S V   VG+ ++G+  +   ++V  F  +L +Y +  RP +LY++ 
Sbjct: 192 DIRAKYLIGADGANSQVVDQVGLPMEGKMGVSGSINV-VFEADLTKY-VGHRPSVLYWVI 251

Query: 309 ----NTEAIGVLVAHDLKQGEFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDV 368
               +   +G+ V   ++     L +  Y       D +     +++  L+G +   + +
Sbjct: 252 QPGSSVGGLGIGVIRMVRPWNKWLCIWGYDIAGGPPDLNEAHARQIVHSLLGDSTIPVKI 311

Query: 369 QDVKPWIMHAEVAEKFICCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQ 428
           +    W ++   A +     NRV   GDA HR PP  G G NT IQD  NL WKL+ VLQ
Sbjct: 312 ESTSTWTVNDMYATRLF--DNRVFCMGDAVHRHPPTNGLGSNTSIQDAFNLCWKLSHVLQ 371

Query: 429 DIASPSILNTYEMERRPIAQFNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGS 488
             A P +L TY  ER P+A+                             V Q  N  LG 
Sbjct: 372 GKAGPELLATYNEERAPVAR----------------------------QVVQRANKSLGD 431

Query: 489 VLPSSLQSSVLDGIFKIGRMQLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDL 548
             P  L +  L       +MQ + + L +++P    + A LR   D G          ++
Sbjct: 432 -FPPILAALGLFDTKDPEQMQRNIARLKEQSPEAQEQRAALRAAID-GTQYVYNAHGVEM 491

Query: 549 GFRYSEGALIPDNTLLSGPEEPTGRRRQ---YVPSADPGSRLPHMNVRTLASEDMISTLD 608
             RY   A++PD     G  +P  RR     +  S  PG+ +PH+ V        +STLD
Sbjct: 492 NQRYQSAAIVPD-----GTPDPGFRRDSELYHAHSGRPGAPVPHVWVTRHGRR--VSTLD 551

Query: 609 LVSGDKVEFLLIIAPLPESYHLARSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELT 668
           L    +   L  IA  P        A   AAE     + V ++             QEL 
Sbjct: 552 LCGKGRFSLLSGIAGSPWV-----EAAVHAAESLGIDLDVHIIGPG----------QELE 584

Query: 669 PWENYMDVQEIRQSSTSPSWWDVCRMTDNGAILVRPDEHIAWRV---KSGVSGDLNTQMK 719
             + Y D   +R+            + ++GA+LVRPD  I WR    + G   +L   +K
Sbjct: 612 --DLYGDFARVRE------------IEESGALLVRPDNFICWRAMRWQEGSGDELRAALK 584

BLAST of MC09g1852 vs. ExPASy Swiss-Prot
Match: P27138 (2,4-dichlorophenol 6-monooxygenase OS=Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) OX=264198 GN=tfdB PE=3 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 2.1e-44
Identity = 176/662 (26.59%), Postives = 275/662 (41.54%), Query Frame = 0

Query: 69  VLIVGAGPVGLVLAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRKLHGLAEEI 128
           VL+VG GP G     LL + GV+  ++ K    +  P+AH  N R+ME+ R L GL  E 
Sbjct: 9   VLVVGTGPAGASAGALLARYGVRTMLINKYNWTAPTPRAHITNQRTMEILRDL-GLEAEA 68

Query: 129 QLCQPPVDSWRKFIYCTSLNGAILGSV-----DHMQPQDFGNIISPVSVAHFSQYKLNRL 188
           +L   P D   +   C SL G   G +     D  +  D+    SP S+    Q  L  +
Sbjct: 69  RLYAAPNDLMGENTICASLAGEEFGRIRTWGTDVRRRADYDE-CSPTSMCDLPQNYLEPI 128

Query: 189 LLKKLQNLGFQVCSPDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKHTER 248
           L+K             +  D C VR     LGHE              +S L++  + E 
Sbjct: 129 LVK------------SAALDGCKVRFDTEYLGHE--------QDADGVSSRLRDRLNGEE 188

Query: 249 RNICSNILVGTDGAGSTVRRLVGIEIKGENDLQKLVSVH-FFSRELGEYLLNERPGMLYF 308
             + S  L+G DGA S V  +  +++  E  + K  S++  F  +L  Y+ + RP +LY+
Sbjct: 189 FTVRSKYLIGADGANSRV--VSDLDLPLEGTMGKSGSINLLFEADLDRYVAH-RPSVLYW 248

Query: 309 IF----NTEAIGVLVAHDLKQGEFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDI 368
           +     +   +G+ V   ++     L +  Y  +Q   + S      ++  L+G +   +
Sbjct: 249 VIQPGSDIGGLGIGVVRMVRPWNKWLAIWGYDVEQGPPEISESFARRIVHNLIGDDSVPL 308

Query: 369 DVQDVKPWIMHAEVAEKFICCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAV 428
            ++ +  W ++   A +      RV  AGDA HR PP  G G NT IQD  NLAWK+A V
Sbjct: 309 KIEGISTWTVNDMYATRL--QQGRVFCAGDAVHRHPPTNGLGSNTSIQDSFNLAWKIAMV 368

Query: 429 LQDIASPSILNTYEMERRPIAQFNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGL 488
           L   A  S+L+TY +ER PIA+     + K+ +    +  ALGL                
Sbjct: 369 LNGTADESLLDTYTIERAPIAKQVVCRANKSLEDFPPIAMALGLP--------------- 428

Query: 489 GSVLPSSLQSSVLDGIFKIGRMQLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAE 548
                   Q+   D       M+ + +   +  P   ++  +LR+    G +        
Sbjct: 429 --------QAKSAD------EMKSNMARRKEPGPEAQAQRTRLREAI-AGTNYVYNAHGV 488

Query: 549 DLGFRYSEGALIPDNTLLSGPEEPTGRRRQY-VPSADPGSRLPHMNVRTLASEDMISTLD 608
           ++  RY   A++ DN+    P+E       Y   S  PG+ +PH+ V        IST D
Sbjct: 489 EMNQRYDSPAIVADNS----PDEVFRDVELYHQASTRPGAPMPHVWVYASGDGHRISTKD 548

Query: 609 LVSGDKVEFLLIIAPLPESYHLARSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELT 668
           L    K  F L          +  +A + AA      + V V       R+    Q    
Sbjct: 549 LCG--KGNFTLFTG-------IGGAAWQDAAAAVSRQLGVAV-----TVRIIGPGQAYED 580

Query: 669 PWENYMDVQEIRQSSTSPSWWDVCRMTDNGAILVRPDEHIAWRVKS---GVSGDLNTQMK 717
            + ++  + EI                D GAILVRPD H+A+R  S     +GDL + M+
Sbjct: 609 HYGDFARISEI---------------IDTGAILVRPDFHVAYRATSLPADAAGDLVSAMR 580

BLAST of MC09g1852 vs. ExPASy Swiss-Prot
Match: P39888 (Tetracenomycin polyketide synthesis hydroxylase TcmG OS=Streptomyces glaucescens OX=1907 GN=tcmG PE=3 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 6.8e-35
Identity = 121/386 (31.35%), Postives = 181/386 (46.89%), Query Frame = 0

Query: 67  VPVLIVGAGPVGLVLAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRKLHGLAE 126
           VPVLIVG G  GL  A+ L++ GV C +VEK+R  +   +A  I++R+ME+ R + GL  
Sbjct: 17  VPVLIVGGGLTGLSAALFLSQHGVSCRLVEKHRGTTVLTRASGISSRTMELLRGV-GLER 76

Query: 127 EIQLCQPPV---DSWRKFIYCTSLNGAILGSVDHMQPQDFGNIIS----PVSVAHFSQYK 186
            +    P +     WR+          ++   + +   D  N ++     + V H S  +
Sbjct: 77  TVIERGPKLVEGARWRELGQPADQIPWVVIRANGL--HDLENAVTVEEPSLDVGHLSPTR 136

Query: 187 LNRLLLKKLQNLGFQVCSPDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGK 246
                  +L+ +         L D  + R  +I       +  A +  VT T      G 
Sbjct: 137 PYWCGQDRLEPI---------LRDEAVRRGARIDFNTRMEAFTADESGVTATIVDQATG- 196

Query: 247 HTERRNICSNILVGTDGAGSTVRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGM 306
             E+  + +  L+  DG  S VR  +GI   G   +   +SV  F  +L + +   R  +
Sbjct: 197 --EQSTVRARYLIAADGVRSPVRETLGITRTGHGTIGNAMSV-LFKADLRDTVKGRR-FV 256

Query: 307 LYFIFNTEAIGVL-------VAHDLKQGEFILQVPFYPPQQSIEDFSPKMCEELIFKLVG 366
           + ++ N +  GVL       V     +  +I    F P + S E F+ + C ++I    G
Sbjct: 257 ICYLPNPDEPGVLQLPEVPAVLQLFDEDRWIFGFFFDPRETSPEQFTDERCAQIIRTATG 316

Query: 367 LNLCDIDVQDVKPWIMHAEVAEKFICCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLA 426
           L    ++VQ  +PW M    A  +     RV LAGDAAH  PPAG FG N GIQD HNLA
Sbjct: 317 LPGLPVEVQMARPWEMSHNSARSY--RSGRVFLAGDAAHVHPPAGAFGANGGIQDAHNLA 376

Query: 427 WKLAAVLQDIASPSILNTYEMERRPI 439
           WKLAAVL+  A  ++L+TYE ER PI
Sbjct: 377 WKLAAVLKGTAGDALLDTYEQERLPI 383

BLAST of MC09g1852 vs. NCBI nr
Match: XP_022151935.1 (uncharacterized protein LOC111019789 [Momordica charantia])

HSP 1 Score: 1444 bits (3739), Expect = 0.0
Identity = 722/722 (100.00%), Postives = 722/722 (100.00%), Query Frame = 0

Query: 1   MTVSFQNVDGDLLSLFSIRVMGLLGFVNRFNGLRKFDAKLRALPLGLVQSRGLSNSKIFH 60
           MTVSFQNVDGDLLSLFSIRVMGLLGFVNRFNGLRKFDAKLRALPLGLVQSRGLSNSKIFH
Sbjct: 1   MTVSFQNVDGDLLSLFSIRVMGLLGFVNRFNGLRKFDAKLRALPLGLVQSRGLSNSKIFH 60

Query: 61  GGEDTMVPVLIVGAGPVGLVLAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRK 120
           GGEDTMVPVLIVGAGPVGLVLAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRK
Sbjct: 61  GGEDTMVPVLIVGAGPVGLVLAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRK 120

Query: 121 LHGLAEEIQLCQPPVDSWRKFIYCTSLNGAILGSVDHMQPQDFGNIISPVSVAHFSQYKL 180
           LHGLAEEIQLCQPPVDSWRKFIYCTSLNGAILGSVDHMQPQDFGNIISPVSVAHFSQYKL
Sbjct: 121 LHGLAEEIQLCQPPVDSWRKFIYCTSLNGAILGSVDHMQPQDFGNIISPVSVAHFSQYKL 180

Query: 181 NRLLLKKLQNLGFQVCSPDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKH 240
           NRLLLKKLQNLGFQVCSPDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKH
Sbjct: 181 NRLLLKKLQNLGFQVCSPDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKH 240

Query: 241 TERRNICSNILVGTDGAGSTVRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGML 300
           TERRNICSNILVGTDGAGSTVRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGML
Sbjct: 241 TERRNICSNILVGTDGAGSTVRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGML 300

Query: 301 YFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDV 360
           YFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDV
Sbjct: 301 YFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDV 360

Query: 361 QDVKPWIMHAEVAEKFICCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQ 420
           QDVKPWIMHAEVAEKFICCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQ
Sbjct: 361 QDVKPWIMHAEVAEKFICCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQ 420

Query: 421 DIASPSILNTYEMERRPIAQFNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGS 480
           DIASPSILNTYEMERRPIAQFNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGS
Sbjct: 421 DIASPSILNTYEMERRPIAQFNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGS 480

Query: 481 VLPSSLQSSVLDGIFKIGRMQLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDL 540
           VLPSSLQSSVLDGIFKIGRMQLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDL
Sbjct: 481 VLPSSLQSSVLDGIFKIGRMQLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDL 540

Query: 541 GFRYSEGALIPDNTLLSGPEEPTGRRRQYVPSADPGSRLPHMNVRTLASEDMISTLDLVS 600
           GFRYSEGALIPDNTLLSGPEEPTGRRRQYVPSADPGSRLPHMNVRTLASEDMISTLDLVS
Sbjct: 541 GFRYSEGALIPDNTLLSGPEEPTGRRRQYVPSADPGSRLPHMNVRTLASEDMISTLDLVS 600

Query: 601 GDKVEFLLIIAPLPESYHLARSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELTPWE 660
           GDKVEFLLIIAPLPESYHLARSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELTPWE
Sbjct: 601 GDKVEFLLIIAPLPESYHLARSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELTPWE 660

Query: 661 NYMDVQEIRQSSTSPSWWDVCRMTDNGAILVRPDEHIAWRVKSGVSGDLNTQMKRVFTAL 720
           NYMDVQEIRQSSTSPSWWDVCRMTDNGAILVRPDEHIAWRVKSGVSGDLNTQMKRVFTAL
Sbjct: 661 NYMDVQEIRQSSTSPSWWDVCRMTDNGAILVRPDEHIAWRVKSGVSGDLNTQMKRVFTAL 720

Query: 721 LK 722
           LK
Sbjct: 721 LK 722

BLAST of MC09g1852 vs. NCBI nr
Match: KAG7012746.1 (hypothetical protein SDJN02_25499, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1252 bits (3240), Expect = 0.0
Identity = 619/722 (85.73%), Postives = 665/722 (92.11%), Query Frame = 0

Query: 2   TVSFQNVDGDLLSLFSIRVMGLLGFVNRFNGLRKFDAKLRALPLGLVQSRGLSNSKIFHG 61
           TV+  NVDGDLLSL S+  MG LGF  RFNGL+KF+A LRA+PLG VQSRGLSNSK+FHG
Sbjct: 15  TVNCLNVDGDLLSLLSVTAMGFLGFFKRFNGLQKFNASLRAIPLGYVQSRGLSNSKLFHG 74

Query: 62  GEDTMVPVLIVGAGPVGLVLAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRKL 121
           GE+T VPVLIVGAGPVGLVLAILLTKLGVKCA++EKN SFSNHPQAHFINNRSMEVFRKL
Sbjct: 75  GEETAVPVLIVGAGPVGLVLAILLTKLGVKCAILEKNTSFSNHPQAHFINNRSMEVFRKL 134

Query: 122 HGLAEEIQLCQPPVDSWRKFIYCTSLNGAILGSVDHMQPQDFGNIISPVSVAHFSQYKLN 181
            GLAEEIQL QPPVDSWRKFIYCTSL G ILGSVDHMQPQDF +IISPVSVAHFSQYKLN
Sbjct: 135 DGLAEEIQLRQPPVDSWRKFIYCTSLKGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLN 194

Query: 182 RLLLKKLQNLGFQVCSPDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKHT 241
           RLLLK+LQNLGFQVCSPDS E  CIVREKQIL+GHECVSI  TDD+VT TASYLKEGKH 
Sbjct: 195 RLLLKQLQNLGFQVCSPDSSEGPCIVREKQILMGHECVSIGVTDDAVTMTASYLKEGKHI 254

Query: 242 ERRNICSNILVGTDGAGSTVRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGMLY 301
           ERRNICSNILVG DGAGSTVRRLVGIE+KGE DLQKLVS+HFFSRELGEYLL ERPGMLY
Sbjct: 255 ERRNICSNILVGADGAGSTVRRLVGIEMKGEKDLQKLVSIHFFSRELGEYLLTERPGMLY 314

Query: 302 FIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDVQ 361
           FIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQ+IEDF PKMCEE+IF LVGLNLCD+DVQ
Sbjct: 315 FIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIEDFCPKMCEEIIFDLVGLNLCDVDVQ 374

Query: 362 DVKPWIMHAEVAEKFICCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQD 421
           DVKPWIMHAEVAEKFI C  RVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQD
Sbjct: 375 DVKPWIMHAEVAEKFIFCEGRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQD 434

Query: 422 IASPSILNTYEMERRPIAQFNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGSV 481
           IASPSILNTYEMER+PIA  NTALSVKNFKAAME+PAALGLDP+IANSVHQAVNHG+GSV
Sbjct: 435 IASPSILNTYEMERKPIALLNTALSVKNFKAAMEIPAALGLDPKIANSVHQAVNHGIGSV 494

Query: 482 LPSSLQSSVLDGIFKIGRMQLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDLG 541
           L SSLQ +VLDGIFKIGR+QLS++LLNDKNP+GSSRLAKL  IFDEGKSLQLQFPAEDLG
Sbjct: 495 LSSSLQRTVLDGIFKIGRLQLSDTLLNDKNPVGSSRLAKLSHIFDEGKSLQLQFPAEDLG 554

Query: 542 FRYSEGALIPDNTLLSGPEEPTGRRRQYVPSADPGSRLPHMNVRTLASEDMISTLDLVSG 601
           FRYSEGAL+PDN    G EEPTGRRR+Y+PSADPGS+LPHMNVR LAS ++ISTLDLVSG
Sbjct: 555 FRYSEGALLPDNNQPGGREEPTGRRRRYIPSADPGSKLPHMNVRALASMEVISTLDLVSG 614

Query: 602 DKVEFLLIIAPLPESYHLARSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELTPWEN 661
           DKVEFLLIIAPLPESY LAR+AL +A EEFKTSV+VC+LWSADI R+ES S++ELTPWEN
Sbjct: 615 DKVEFLLIIAPLPESYRLARAALMVA-EEFKTSVRVCILWSADITRIESSSKEELTPWEN 674

Query: 662 YMDVQEIRQSSTSP-SWWDVCRMTDNGAILVRPDEHIAWRVKSGVSGDLNTQMKRVFTAL 721
           Y+DVQEIRQ STS  SWWDVC+MTD GAILVRPDEH+ WRVKSGVSGD NT+++RVFT L
Sbjct: 675 YIDVQEIRQPSTSTASWWDVCQMTDKGAILVRPDEHVGWRVKSGVSGDPNTELRRVFTTL 734

BLAST of MC09g1852 vs. NCBI nr
Match: XP_022945741.1 (uncharacterized protein LOC111449881 [Cucurbita moschata])

HSP 1 Score: 1251 bits (3238), Expect = 0.0
Identity = 621/722 (86.01%), Postives = 664/722 (91.97%), Query Frame = 0

Query: 2   TVSFQNVDGDLLSLFSIRVMGLLGFVNRFNGLRKFDAKLRALPLGLVQSRGLSNSKIFHG 61
           TV+  NVDGDLLSLFS+  MG LGF  RFNGL+KF+A LRA+PLG VQSRGLSNSK+FHG
Sbjct: 15  TVNCLNVDGDLLSLFSVTDMGFLGFFKRFNGLQKFNASLRAIPLGYVQSRGLSNSKLFHG 74

Query: 62  GEDTMVPVLIVGAGPVGLVLAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRKL 121
           GE+T VPVLIVGAGPVGLVLAILLTKLGVKCA++EKN SFSNHPQAHFINNRSMEVFRKL
Sbjct: 75  GEETAVPVLIVGAGPVGLVLAILLTKLGVKCAILEKNTSFSNHPQAHFINNRSMEVFRKL 134

Query: 122 HGLAEEIQLCQPPVDSWRKFIYCTSLNGAILGSVDHMQPQDFGNIISPVSVAHFSQYKLN 181
            GLAEEIQL QPPVDSWRKFIYCTSL G ILGSVDHMQPQDF +IISPVSVAHFSQYKLN
Sbjct: 135 DGLAEEIQLRQPPVDSWRKFIYCTSLKGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLN 194

Query: 182 RLLLKKLQNLGFQVCSPDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKHT 241
           RLLLK+LQNL FQVCSPDS E  CIVREKQIL+GHECVSI  TDD+VT TASYLKEGKH 
Sbjct: 195 RLLLKQLQNLEFQVCSPDSSEGPCIVREKQILMGHECVSIGVTDDAVTMTASYLKEGKHI 254

Query: 242 ERRNICSNILVGTDGAGSTVRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGMLY 301
           ERRNICSNILVG DGAGSTVRRLVGIE+KGE DLQKLVS+HFFSRELGEYLL ERPGMLY
Sbjct: 255 ERRNICSNILVGADGAGSTVRRLVGIEMKGEKDLQKLVSIHFFSRELGEYLLTERPGMLY 314

Query: 302 FIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDVQ 361
           FIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQ+IEDF PKMCEE+IF LVGLNLCD+DVQ
Sbjct: 315 FIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIEDFCPKMCEEIIFDLVGLNLCDVDVQ 374

Query: 362 DVKPWIMHAEVAEKFICCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQD 421
           DVKPWIMHAEVAEKFI C  RVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQD
Sbjct: 375 DVKPWIMHAEVAEKFISCQGRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQD 434

Query: 422 IASPSILNTYEMERRPIAQFNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGSV 481
           IASPSILNTYEMER+PIA  NTALSVKNFKAAMEVPAALGLDP+IANSVHQAVNHGLGSV
Sbjct: 435 IASPSILNTYEMERKPIALLNTALSVKNFKAAMEVPAALGLDPKIANSVHQAVNHGLGSV 494

Query: 482 LPSSLQSSVLDGIFKIGRMQLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDLG 541
           L SSLQ  VLDGIFKIGR+QLS++LLNDKNP+GSSRLAKL  IFDEGKSLQLQFPAEDLG
Sbjct: 495 LSSSLQREVLDGIFKIGRLQLSDTLLNDKNPVGSSRLAKLSHIFDEGKSLQLQFPAEDLG 554

Query: 542 FRYSEGALIPDNTLLSGPEEPTGRRRQYVPSADPGSRLPHMNVRTLASEDMISTLDLVSG 601
           FRYSEGAL+PDN    G EEPTGRRR+Y+PSADPGS+LPHMNVR LAS ++ISTLDLVSG
Sbjct: 555 FRYSEGALLPDNNQPGGREEPTGRRRRYIPSADPGSKLPHMNVRALASMEVISTLDLVSG 614

Query: 602 DKVEFLLIIAPLPESYHLARSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELTPWEN 661
           DKVEFLLIIAPLPESY LAR+AL +A EEFKTSV+VC+LWSADI R+ES S++ELTPWEN
Sbjct: 615 DKVEFLLIIAPLPESYRLARAALMVA-EEFKTSVRVCILWSADITRIESSSKEELTPWEN 674

Query: 662 YMDVQEIRQSSTSP-SWWDVCRMTDNGAILVRPDEHIAWRVKSGVSGDLNTQMKRVFTAL 721
           Y+DVQEIRQ STS  SWWDVC+MTD GAILVRPDEH+ WRVKSGVSGD NT+++RVFT L
Sbjct: 675 YIDVQEIRQPSTSTASWWDVCQMTDKGAILVRPDEHVGWRVKSGVSGDPNTELRRVFTTL 734

BLAST of MC09g1852 vs. NCBI nr
Match: XP_022966729.1 (uncharacterized protein LOC111466353 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1234 bits (3193), Expect = 0.0
Identity = 608/703 (86.49%), Postives = 653/703 (92.89%), Query Frame = 0

Query: 21  MGLLGFVNRFNGLRKFDAKLRALPLGLVQSRGLSNSKIFHGGEDTMVPVLIVGAGPVGLV 80
           MG LGF  RFNGLR+F+A LRA+PLG VQSRGLSNSK+FHGGE+T VPVLIVGAGPVGLV
Sbjct: 1   MGFLGFFKRFNGLRRFNASLRAIPLGYVQSRGLSNSKLFHGGEETAVPVLIVGAGPVGLV 60

Query: 81  LAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRKLHGLAEEIQLCQPPVDSWRK 140
           LAILLTKLGVKCA++EKN SFSNHPQAHFINNRSMEVFRKL GLAEEIQLCQPPVDSWRK
Sbjct: 61  LAILLTKLGVKCAILEKNTSFSNHPQAHFINNRSMEVFRKLDGLAEEIQLCQPPVDSWRK 120

Query: 141 FIYCTSLNGAILGSVDHMQPQDFGNIISPVSVAHFSQYKLNRLLLKKLQNLGFQVCSPDS 200
           F+YCTSL G ILGSVDHMQPQDF +IISPVSVAHFSQYKLNRLLLK+LQNLGFQVCSPDS
Sbjct: 121 FLYCTSLKGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNRLLLKQLQNLGFQVCSPDS 180

Query: 201 LEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKHTERRNICSNILVGTDGAGST 260
            E  CIVREKQIL+GHECVSI  TDD+VT TASYLKEGKH ERRNICSNILVG DGAGST
Sbjct: 181 SEGPCIVREKQILMGHECVSIGVTDDAVTMTASYLKEGKHIERRNICSNILVGADGAGST 240

Query: 261 VRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGMLYFIFNTEAIGVLVAHDLKQG 320
           VRRLVGIE+KGE +LQKLVS+HFFSRELGEYLL ERPGMLYFIFN EAIGVLVAHDLKQG
Sbjct: 241 VRRLVGIEMKGEKNLQKLVSIHFFSRELGEYLLTERPGMLYFIFNIEAIGVLVAHDLKQG 300

Query: 321 EFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDVQDVKPWIMHAEVAEKFICCH 380
           EFILQVPFYPPQQ+IEDF PKMCEE+IF LVGLNLCD+DVQDVKPWIMHAEVAEKFI C 
Sbjct: 301 EFILQVPFYPPQQNIEDFCPKMCEEIIFNLVGLNLCDVDVQDVKPWIMHAEVAEKFIACQ 360

Query: 381 NRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIAQ 440
           NRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMER+PIA 
Sbjct: 361 NRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERKPIAL 420

Query: 441 FNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGSVLPSSLQSSVLDGIFKIGRM 500
            NTALSVKNFKAAMEVPAALGLDP+IANSVHQAVNHGLGSVL SSLQ +VLDGIFKIGR+
Sbjct: 421 LNTALSVKNFKAAMEVPAALGLDPKIANSVHQAVNHGLGSVLSSSLQRTVLDGIFKIGRL 480

Query: 501 QLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDLGFRYSEGALIPDNTLLSGPE 560
           QLS++LLN+KNP+GSSRLAKL  IFDEGKSLQLQFPAEDLGFRYSEGALIPDN  L G E
Sbjct: 481 QLSDTLLNEKNPVGSSRLAKLSHIFDEGKSLQLQFPAEDLGFRYSEGALIPDNNQLGGRE 540

Query: 561 EPTGRRRQYVPSADPGSRLPHMNVRTLASEDMISTLDLVSGDKVEFLLIIAPLPESYHLA 620
           EPTGRRR+Y+PSADPGS+LPHMNVR LAS ++ISTLDLVSGDKVEFLLIIAPLPE Y LA
Sbjct: 541 EPTGRRRRYIPSADPGSKLPHMNVRALASMEVISTLDLVSGDKVEFLLIIAPLPEFYRLA 600

Query: 621 RSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELTPWENYMDVQEIRQSSTSP-SWWD 680
           R+AL +A EEFKTSV+VC+LWSADI R+ES S++EL PWENY+DVQEIRQ STS  SWWD
Sbjct: 601 RAALMVA-EEFKTSVRVCILWSADITRIESSSKEELAPWENYIDVQEIRQPSTSTASWWD 660

Query: 681 VCRMTDNGAILVRPDEHIAWRVKSGVSGDLNTQMKRVFTALLK 722
           VC+MTD GAILVRPDEH+AWRVKSGVSGD NT+++RVFT+LLK
Sbjct: 661 VCQMTDKGAILVRPDEHVAWRVKSGVSGDPNTELRRVFTSLLK 702

BLAST of MC09g1852 vs. NCBI nr
Match: XP_023541526.1 (uncharacterized protein LOC111801672 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1231 bits (3184), Expect = 0.0
Identity = 609/703 (86.63%), Postives = 650/703 (92.46%), Query Frame = 0

Query: 21  MGLLGFVNRFNGLRKFDAKLRALPLGLVQSRGLSNSKIFHGGEDTMVPVLIVGAGPVGLV 80
           MG LGF  RFNGL+KFDA LRALPLG VQSRGLSNSK+FHGGE+T VPVLIVGAGPVGLV
Sbjct: 1   MGFLGFFKRFNGLQKFDASLRALPLGYVQSRGLSNSKLFHGGEETAVPVLIVGAGPVGLV 60

Query: 81  LAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRKLHGLAEEIQLCQPPVDSWRK 140
           LAILLTKLGVKCA++EKN SFSNHPQAHFINNRSMEVFRKL GLAEEIQL QPPVDSWRK
Sbjct: 61  LAILLTKLGVKCAILEKNTSFSNHPQAHFINNRSMEVFRKLDGLAEEIQLRQPPVDSWRK 120

Query: 141 FIYCTSLNGAILGSVDHMQPQDFGNIISPVSVAHFSQYKLNRLLLKKLQNLGFQVCSPDS 200
           FIYCTSL G ILGSVDHMQPQDF +IISPVSVAHFSQYKLNRLLLK+LQNLGFQVCSPDS
Sbjct: 121 FIYCTSLKGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNRLLLKQLQNLGFQVCSPDS 180

Query: 201 LEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKHTERRNICSNILVGTDGAGST 260
            E  CIVREKQIL+GHECVSI  TDD+VT TASYLKEGKH ERRNICSNILVG DGAGST
Sbjct: 181 SEGPCIVREKQILMGHECVSIGVTDDAVTMTASYLKEGKHIERRNICSNILVGADGAGST 240

Query: 261 VRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGMLYFIFNTEAIGVLVAHDLKQG 320
           VRRLVGIE+KGE DLQKLVS+HFFSRELGEYLL ERPGMLYFIFNTEAIGVLVAHDLKQG
Sbjct: 241 VRRLVGIEMKGEKDLQKLVSIHFFSRELGEYLLTERPGMLYFIFNTEAIGVLVAHDLKQG 300

Query: 321 EFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDVQDVKPWIMHAEVAEKFICCH 380
           EFILQVPFYPPQQ+IEDF PKMCEE+IF LVGLNLCD+DVQDVKPWIMHAEVAEKFI C 
Sbjct: 301 EFILQVPFYPPQQNIEDFCPKMCEEIIFDLVGLNLCDVDVQDVKPWIMHAEVAEKFISCQ 360

Query: 381 NRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIAQ 440
            RVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMER+PIA 
Sbjct: 361 GRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERKPIAL 420

Query: 441 FNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGSVLPSSLQSSVLDGIFKIGRM 500
            NTALSVKNFKAAME+PAALGLDP+IANSVHQAVNHGLGSVL SSLQ +VLDGIFKIGR+
Sbjct: 421 LNTALSVKNFKAAMEIPAALGLDPKIANSVHQAVNHGLGSVLSSSLQRTVLDGIFKIGRL 480

Query: 501 QLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDLGFRYSEGALIPDNTLLSGPE 560
           QLS++LLNDKNP+GSSRLAKL  IF EGKSLQLQFPAEDLGFRYSEGALIPDN    G E
Sbjct: 481 QLSDTLLNDKNPVGSSRLAKLSHIFHEGKSLQLQFPAEDLGFRYSEGALIPDNNQPGGRE 540

Query: 561 EPTGRRRQYVPSADPGSRLPHMNVRTLASEDMISTLDLVSGDKVEFLLIIAPLPESYHLA 620
           EPTGRRR+Y+PSADPGS+LPHMNVR LAS ++ISTLDLVSGDKVEFLLIIAPLP+SY LA
Sbjct: 541 EPTGRRRRYIPSADPGSKLPHMNVRALASMEVISTLDLVSGDKVEFLLIIAPLPDSYRLA 600

Query: 621 RSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELTPWENYMDVQEIRQSSTSP-SWWD 680
           R+AL +A EEFKTSV+VC+LWSADI R+ES S++ELTPWENY+DVQEIRQ STS  SWWD
Sbjct: 601 RAALMVA-EEFKTSVRVCILWSADITRIESSSKEELTPWENYIDVQEIRQPSTSAASWWD 660

Query: 681 VCRMTDNGAILVRPDEHIAWRVKSGVSGDLNTQMKRVFTALLK 722
           VC+MTD GAILVRPDEH+ WRVKSGVSGD NT+++RVFT LLK
Sbjct: 661 VCQMTDKGAILVRPDEHVGWRVKSGVSGDPNTELRRVFTTLLK 702

BLAST of MC09g1852 vs. ExPASy TrEMBL
Match: A0A6J1DCK1 (uncharacterized protein LOC111019789 OS=Momordica charantia OX=3673 GN=LOC111019789 PE=4 SV=1)

HSP 1 Score: 1444 bits (3739), Expect = 0.0
Identity = 722/722 (100.00%), Postives = 722/722 (100.00%), Query Frame = 0

Query: 1   MTVSFQNVDGDLLSLFSIRVMGLLGFVNRFNGLRKFDAKLRALPLGLVQSRGLSNSKIFH 60
           MTVSFQNVDGDLLSLFSIRVMGLLGFVNRFNGLRKFDAKLRALPLGLVQSRGLSNSKIFH
Sbjct: 1   MTVSFQNVDGDLLSLFSIRVMGLLGFVNRFNGLRKFDAKLRALPLGLVQSRGLSNSKIFH 60

Query: 61  GGEDTMVPVLIVGAGPVGLVLAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRK 120
           GGEDTMVPVLIVGAGPVGLVLAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRK
Sbjct: 61  GGEDTMVPVLIVGAGPVGLVLAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRK 120

Query: 121 LHGLAEEIQLCQPPVDSWRKFIYCTSLNGAILGSVDHMQPQDFGNIISPVSVAHFSQYKL 180
           LHGLAEEIQLCQPPVDSWRKFIYCTSLNGAILGSVDHMQPQDFGNIISPVSVAHFSQYKL
Sbjct: 121 LHGLAEEIQLCQPPVDSWRKFIYCTSLNGAILGSVDHMQPQDFGNIISPVSVAHFSQYKL 180

Query: 181 NRLLLKKLQNLGFQVCSPDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKH 240
           NRLLLKKLQNLGFQVCSPDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKH
Sbjct: 181 NRLLLKKLQNLGFQVCSPDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKH 240

Query: 241 TERRNICSNILVGTDGAGSTVRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGML 300
           TERRNICSNILVGTDGAGSTVRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGML
Sbjct: 241 TERRNICSNILVGTDGAGSTVRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGML 300

Query: 301 YFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDV 360
           YFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDV
Sbjct: 301 YFIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDV 360

Query: 361 QDVKPWIMHAEVAEKFICCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQ 420
           QDVKPWIMHAEVAEKFICCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQ
Sbjct: 361 QDVKPWIMHAEVAEKFICCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQ 420

Query: 421 DIASPSILNTYEMERRPIAQFNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGS 480
           DIASPSILNTYEMERRPIAQFNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGS
Sbjct: 421 DIASPSILNTYEMERRPIAQFNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGS 480

Query: 481 VLPSSLQSSVLDGIFKIGRMQLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDL 540
           VLPSSLQSSVLDGIFKIGRMQLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDL
Sbjct: 481 VLPSSLQSSVLDGIFKIGRMQLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDL 540

Query: 541 GFRYSEGALIPDNTLLSGPEEPTGRRRQYVPSADPGSRLPHMNVRTLASEDMISTLDLVS 600
           GFRYSEGALIPDNTLLSGPEEPTGRRRQYVPSADPGSRLPHMNVRTLASEDMISTLDLVS
Sbjct: 541 GFRYSEGALIPDNTLLSGPEEPTGRRRQYVPSADPGSRLPHMNVRTLASEDMISTLDLVS 600

Query: 601 GDKVEFLLIIAPLPESYHLARSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELTPWE 660
           GDKVEFLLIIAPLPESYHLARSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELTPWE
Sbjct: 601 GDKVEFLLIIAPLPESYHLARSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELTPWE 660

Query: 661 NYMDVQEIRQSSTSPSWWDVCRMTDNGAILVRPDEHIAWRVKSGVSGDLNTQMKRVFTAL 720
           NYMDVQEIRQSSTSPSWWDVCRMTDNGAILVRPDEHIAWRVKSGVSGDLNTQMKRVFTAL
Sbjct: 661 NYMDVQEIRQSSTSPSWWDVCRMTDNGAILVRPDEHIAWRVKSGVSGDLNTQMKRVFTAL 720

Query: 721 LK 722
           LK
Sbjct: 721 LK 722

BLAST of MC09g1852 vs. ExPASy TrEMBL
Match: A0A6J1G1R0 (uncharacterized protein LOC111449881 OS=Cucurbita moschata OX=3662 GN=LOC111449881 PE=4 SV=1)

HSP 1 Score: 1251 bits (3238), Expect = 0.0
Identity = 621/722 (86.01%), Postives = 664/722 (91.97%), Query Frame = 0

Query: 2   TVSFQNVDGDLLSLFSIRVMGLLGFVNRFNGLRKFDAKLRALPLGLVQSRGLSNSKIFHG 61
           TV+  NVDGDLLSLFS+  MG LGF  RFNGL+KF+A LRA+PLG VQSRGLSNSK+FHG
Sbjct: 15  TVNCLNVDGDLLSLFSVTDMGFLGFFKRFNGLQKFNASLRAIPLGYVQSRGLSNSKLFHG 74

Query: 62  GEDTMVPVLIVGAGPVGLVLAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRKL 121
           GE+T VPVLIVGAGPVGLVLAILLTKLGVKCA++EKN SFSNHPQAHFINNRSMEVFRKL
Sbjct: 75  GEETAVPVLIVGAGPVGLVLAILLTKLGVKCAILEKNTSFSNHPQAHFINNRSMEVFRKL 134

Query: 122 HGLAEEIQLCQPPVDSWRKFIYCTSLNGAILGSVDHMQPQDFGNIISPVSVAHFSQYKLN 181
            GLAEEIQL QPPVDSWRKFIYCTSL G ILGSVDHMQPQDF +IISPVSVAHFSQYKLN
Sbjct: 135 DGLAEEIQLRQPPVDSWRKFIYCTSLKGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLN 194

Query: 182 RLLLKKLQNLGFQVCSPDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKHT 241
           RLLLK+LQNL FQVCSPDS E  CIVREKQIL+GHECVSI  TDD+VT TASYLKEGKH 
Sbjct: 195 RLLLKQLQNLEFQVCSPDSSEGPCIVREKQILMGHECVSIGVTDDAVTMTASYLKEGKHI 254

Query: 242 ERRNICSNILVGTDGAGSTVRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGMLY 301
           ERRNICSNILVG DGAGSTVRRLVGIE+KGE DLQKLVS+HFFSRELGEYLL ERPGMLY
Sbjct: 255 ERRNICSNILVGADGAGSTVRRLVGIEMKGEKDLQKLVSIHFFSRELGEYLLTERPGMLY 314

Query: 302 FIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDVQ 361
           FIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQ+IEDF PKMCEE+IF LVGLNLCD+DVQ
Sbjct: 315 FIFNTEAIGVLVAHDLKQGEFILQVPFYPPQQNIEDFCPKMCEEIIFDLVGLNLCDVDVQ 374

Query: 362 DVKPWIMHAEVAEKFICCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQD 421
           DVKPWIMHAEVAEKFI C  RVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQD
Sbjct: 375 DVKPWIMHAEVAEKFISCQGRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQD 434

Query: 422 IASPSILNTYEMERRPIAQFNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGSV 481
           IASPSILNTYEMER+PIA  NTALSVKNFKAAMEVPAALGLDP+IANSVHQAVNHGLGSV
Sbjct: 435 IASPSILNTYEMERKPIALLNTALSVKNFKAAMEVPAALGLDPKIANSVHQAVNHGLGSV 494

Query: 482 LPSSLQSSVLDGIFKIGRMQLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDLG 541
           L SSLQ  VLDGIFKIGR+QLS++LLNDKNP+GSSRLAKL  IFDEGKSLQLQFPAEDLG
Sbjct: 495 LSSSLQREVLDGIFKIGRLQLSDTLLNDKNPVGSSRLAKLSHIFDEGKSLQLQFPAEDLG 554

Query: 542 FRYSEGALIPDNTLLSGPEEPTGRRRQYVPSADPGSRLPHMNVRTLASEDMISTLDLVSG 601
           FRYSEGAL+PDN    G EEPTGRRR+Y+PSADPGS+LPHMNVR LAS ++ISTLDLVSG
Sbjct: 555 FRYSEGALLPDNNQPGGREEPTGRRRRYIPSADPGSKLPHMNVRALASMEVISTLDLVSG 614

Query: 602 DKVEFLLIIAPLPESYHLARSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELTPWEN 661
           DKVEFLLIIAPLPESY LAR+AL +A EEFKTSV+VC+LWSADI R+ES S++ELTPWEN
Sbjct: 615 DKVEFLLIIAPLPESYRLARAALMVA-EEFKTSVRVCILWSADITRIESSSKEELTPWEN 674

Query: 662 YMDVQEIRQSSTSP-SWWDVCRMTDNGAILVRPDEHIAWRVKSGVSGDLNTQMKRVFTAL 721
           Y+DVQEIRQ STS  SWWDVC+MTD GAILVRPDEH+ WRVKSGVSGD NT+++RVFT L
Sbjct: 675 YIDVQEIRQPSTSTASWWDVCQMTDKGAILVRPDEHVGWRVKSGVSGDPNTELRRVFTTL 734

BLAST of MC09g1852 vs. ExPASy TrEMBL
Match: A0A6J1HUM9 (uncharacterized protein LOC111466353 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466353 PE=4 SV=1)

HSP 1 Score: 1234 bits (3193), Expect = 0.0
Identity = 608/703 (86.49%), Postives = 653/703 (92.89%), Query Frame = 0

Query: 21  MGLLGFVNRFNGLRKFDAKLRALPLGLVQSRGLSNSKIFHGGEDTMVPVLIVGAGPVGLV 80
           MG LGF  RFNGLR+F+A LRA+PLG VQSRGLSNSK+FHGGE+T VPVLIVGAGPVGLV
Sbjct: 1   MGFLGFFKRFNGLRRFNASLRAIPLGYVQSRGLSNSKLFHGGEETAVPVLIVGAGPVGLV 60

Query: 81  LAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRKLHGLAEEIQLCQPPVDSWRK 140
           LAILLTKLGVKCA++EKN SFSNHPQAHFINNRSMEVFRKL GLAEEIQLCQPPVDSWRK
Sbjct: 61  LAILLTKLGVKCAILEKNTSFSNHPQAHFINNRSMEVFRKLDGLAEEIQLCQPPVDSWRK 120

Query: 141 FIYCTSLNGAILGSVDHMQPQDFGNIISPVSVAHFSQYKLNRLLLKKLQNLGFQVCSPDS 200
           F+YCTSL G ILGSVDHMQPQDF +IISPVSVAHFSQYKLNRLLLK+LQNLGFQVCSPDS
Sbjct: 121 FLYCTSLKGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNRLLLKQLQNLGFQVCSPDS 180

Query: 201 LEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKHTERRNICSNILVGTDGAGST 260
            E  CIVREKQIL+GHECVSI  TDD+VT TASYLKEGKH ERRNICSNILVG DGAGST
Sbjct: 181 SEGPCIVREKQILMGHECVSIGVTDDAVTMTASYLKEGKHIERRNICSNILVGADGAGST 240

Query: 261 VRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGMLYFIFNTEAIGVLVAHDLKQG 320
           VRRLVGIE+KGE +LQKLVS+HFFSRELGEYLL ERPGMLYFIFN EAIGVLVAHDLKQG
Sbjct: 241 VRRLVGIEMKGEKNLQKLVSIHFFSRELGEYLLTERPGMLYFIFNIEAIGVLVAHDLKQG 300

Query: 321 EFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDVQDVKPWIMHAEVAEKFICCH 380
           EFILQVPFYPPQQ+IEDF PKMCEE+IF LVGLNLCD+DVQDVKPWIMHAEVAEKFI C 
Sbjct: 301 EFILQVPFYPPQQNIEDFCPKMCEEIIFNLVGLNLCDVDVQDVKPWIMHAEVAEKFIACQ 360

Query: 381 NRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIAQ 440
           NRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMER+PIA 
Sbjct: 361 NRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERKPIAL 420

Query: 441 FNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGSVLPSSLQSSVLDGIFKIGRM 500
            NTALSVKNFKAAMEVPAALGLDP+IANSVHQAVNHGLGSVL SSLQ +VLDGIFKIGR+
Sbjct: 421 LNTALSVKNFKAAMEVPAALGLDPKIANSVHQAVNHGLGSVLSSSLQRTVLDGIFKIGRL 480

Query: 501 QLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDLGFRYSEGALIPDNTLLSGPE 560
           QLS++LLN+KNP+GSSRLAKL  IFDEGKSLQLQFPAEDLGFRYSEGALIPDN  L G E
Sbjct: 481 QLSDTLLNEKNPVGSSRLAKLSHIFDEGKSLQLQFPAEDLGFRYSEGALIPDNNQLGGRE 540

Query: 561 EPTGRRRQYVPSADPGSRLPHMNVRTLASEDMISTLDLVSGDKVEFLLIIAPLPESYHLA 620
           EPTGRRR+Y+PSADPGS+LPHMNVR LAS ++ISTLDLVSGDKVEFLLIIAPLPE Y LA
Sbjct: 541 EPTGRRRRYIPSADPGSKLPHMNVRALASMEVISTLDLVSGDKVEFLLIIAPLPEFYRLA 600

Query: 621 RSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELTPWENYMDVQEIRQSSTSP-SWWD 680
           R+AL +A EEFKTSV+VC+LWSADI R+ES S++EL PWENY+DVQEIRQ STS  SWWD
Sbjct: 601 RAALMVA-EEFKTSVRVCILWSADITRIESSSKEELAPWENYIDVQEIRQPSTSTASWWD 660

Query: 681 VCRMTDNGAILVRPDEHIAWRVKSGVSGDLNTQMKRVFTALLK 722
           VC+MTD GAILVRPDEH+AWRVKSGVSGD NT+++RVFT+LLK
Sbjct: 661 VCQMTDKGAILVRPDEHVAWRVKSGVSGDPNTELRRVFTSLLK 702

BLAST of MC09g1852 vs. ExPASy TrEMBL
Match: A0A1S3BZD5 (putative polyketide hydroxylase OS=Cucumis melo OX=3656 GN=LOC103495067 PE=4 SV=1)

HSP 1 Score: 1229 bits (3181), Expect = 0.0
Identity = 605/710 (85.21%), Postives = 652/710 (91.83%), Query Frame = 0

Query: 13  LSLFSIRVMGLLGFVNRFNGLRKFDAKLRALPLGLVQSRGLSNSKIFHGGEDTMVPVLIV 72
           +SLF  R MG LGF  RFNGL+KFDA  RA+PLG VQ RG SNSK+FHGG++TMVPVLIV
Sbjct: 1   MSLFLNRAMGFLGFFRRFNGLQKFDATPRAIPLGYVQCRGSSNSKLFHGGDETMVPVLIV 60

Query: 73  GAGPVGLVLAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRKLHGLAEEIQLCQ 132
           GAGPVGLVLAILLTKLG+KCA+VEKNRSFS HPQAHFINNR+MEVFRKL GLAE+IQL Q
Sbjct: 61  GAGPVGLVLAILLTKLGIKCAIVEKNRSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQ 120

Query: 133 PPVDSWRKFIYCTSLNGAILGSVDHMQPQDFGNIISPVSVAHFSQYKLNRLLLKKLQNLG 192
           PPV+SWRKFIYCTSLNG ILGSVDHMQPQDF +IISPVSVAHFSQYKLNRLLLK+LQNLG
Sbjct: 121 PPVESWRKFIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNRLLLKQLQNLG 180

Query: 193 FQVCSPDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKHTERRNICSNILV 252
           FQV SPDSLE  C+VREK+ILLGHECVSIDATD+SV  TASYLKEGKH ERRNI  NILV
Sbjct: 181 FQVGSPDSLEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILV 240

Query: 253 GTDGAGSTVRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGMLYFIFNTEAIGVL 312
           G DGAGSTVRRLVG+E+KGENDLQKLVS+HFFSRELGEYLL +RPGMLYFIFNTEAIGVL
Sbjct: 241 GADGAGSTVRRLVGVEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVL 300

Query: 313 VAHDLKQGEFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDVQDVKPWIMHAEV 372
           VAHDLKQGEFILQVPFYPPQQ+IEDF P MC ELIFKLVG NLCDIDVQDVKPWIMHAEV
Sbjct: 301 VAHDLKQGEFILQVPFYPPQQNIEDFCPAMCNELIFKLVGQNLCDIDVQDVKPWIMHAEV 360

Query: 373 AEKFICCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE 432
           AEKFICC N VLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE
Sbjct: 361 AEKFICCRNHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYE 420

Query: 433 MERRPIAQFNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGSVLPSSLQSSVLD 492
           MERRPIA FNTALSVKNFKAAMEVPAALGLDP+IANSVH+ VN+GLGS+L SSLQS+VLD
Sbjct: 421 MERRPIALFNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNNGLGSILSSSLQSAVLD 480

Query: 493 GIFKIGRMQLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDLGFRYSEGALIPD 552
           GIFKIGR+QLS+  LN KNPIGSSRLAKLR IFDEGKSLQLQFPAEDLGFRYS+GA+IPD
Sbjct: 481 GIFKIGRLQLSDIFLNVKNPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSDGAIIPD 540

Query: 553 NTLLSGPEEPTGRRRQYVPSADPGSRLPHMNVRTLASEDMISTLDLVSGDKVEFLLIIAP 612
           NTLL G EEPTGRRRQY+PSADPGSRLPHMNVR LASED+ISTLDLVSGDK+EFLLIIAP
Sbjct: 541 NTLLGGREEPTGRRRQYIPSADPGSRLPHMNVRVLASEDIISTLDLVSGDKIEFLLIIAP 600

Query: 613 LPESYHLARSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELTPWENYMDVQEIRQSS 672
             ESYHLA +  K+A EEFKTSVKVC+LWSA   ++ES S+  LTPWENY+DV+EIRQS+
Sbjct: 601 RSESYHLAHAGFKVA-EEFKTSVKVCILWSASTTKIESSSKDLLTPWENYVDVEEIRQST 660

Query: 673 TSPSWWDVCRMTDNGAILVRPDEHIAWRVKSGVSGDLNTQMKRVFTALLK 722
           TSPSWWD+C+MTD GAILVRPDEHIAWRVKSG+SGD NT++ RVFT LLK
Sbjct: 661 TSPSWWDICKMTDKGAILVRPDEHIAWRVKSGISGDPNTELMRVFTTLLK 709

BLAST of MC09g1852 vs. ExPASy TrEMBL
Match: A0A0A0KLD9 (FAD_binding_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G056620 PE=4 SV=1)

HSP 1 Score: 1211 bits (3134), Expect = 0.0
Identity = 599/702 (85.33%), Postives = 646/702 (92.02%), Query Frame = 0

Query: 21  MGLLGFVNRFNGLRKFDAKLRALPLGLVQSRGLSNSKIFHGGEDTMVPVLIVGAGPVGLV 80
           MG LGF  RFNGL+KFDA LR  PL  +Q RG SNSKIFHGG++TMVPVLIVGAGPVGLV
Sbjct: 1   MGFLGFFKRFNGLQKFDAMLRTKPLRNIQCRGSSNSKIFHGGDETMVPVLIVGAGPVGLV 60

Query: 81  LAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRKLHGLAEEIQLCQPPVDSWRK 140
           LAILLTKLGVKCA+VEKN+SFS HPQAHFINNR+MEVFRKL GLAE+IQL QPPV+SWRK
Sbjct: 61  LAILLTKLGVKCAIVEKNKSFSKHPQAHFINNRTMEVFRKLDGLAEKIQLYQPPVESWRK 120

Query: 141 FIYCTSLNGAILGSVDHMQPQDFGNIISPVSVAHFSQYKLNRLLLKKLQNLGFQVCSPDS 200
           FIYCTSLNG ILGSVDHMQPQDF +IISPVSVAHFSQYKLN LLLK+LQNLGFQVCSPDS
Sbjct: 121 FIYCTSLNGTILGSVDHMQPQDFEHIISPVSVAHFSQYKLNGLLLKQLQNLGFQVCSPDS 180

Query: 201 LEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKHTERRNICSNILVGTDGAGST 260
           LE  C+VREK+ILLGHECVSIDATD+SV  TASYLKEGKH ERRNI  NILVG DGAGST
Sbjct: 181 LEGPCVVREKKILLGHECVSIDATDESVNMTASYLKEGKHVERRNISCNILVGADGAGST 240

Query: 261 VRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGMLYFIFNTEAIGVLVAHDLKQG 320
           VRRLVGIE+KGENDLQKLVS+HFFSRELGEYLL +RPGMLYFIFNTEAIGVLVAHDLKQG
Sbjct: 241 VRRLVGIEMKGENDLQKLVSIHFFSRELGEYLLKDRPGMLYFIFNTEAIGVLVAHDLKQG 300

Query: 321 EFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDVQDVKPWIMHAEVAEKFICCH 380
           EFILQVPFYPPQQ+IEDF P+MCEELIFKLVG NLCDIDV+DVKPWIMHAEVAEKFIC  
Sbjct: 301 EFILQVPFYPPQQNIEDFFPQMCEELIFKLVGRNLCDIDVRDVKPWIMHAEVAEKFICRQ 360

Query: 381 NRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIAQ 440
           N VLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIA 
Sbjct: 361 NHVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRPIAL 420

Query: 441 FNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGSVLPSSLQSSVLDGIFKIGRM 500
           FNTALSVKNFKAAMEVPAALGLDP+IANSVH+ VNHGLGS+L SSLQS+VLDGIFKIGR+
Sbjct: 421 FNTALSVKNFKAAMEVPAALGLDPKIANSVHRVVNHGLGSILSSSLQSAVLDGIFKIGRL 480

Query: 501 QLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDLGFRYSEGALIPDNTLLSGPE 560
           QLS++ LN +NPIGSSRLAKLR IFDEGKSLQLQFPAEDLGFRYSEGA+I DN LL G E
Sbjct: 481 QLSDTFLNVENPIGSSRLAKLRHIFDEGKSLQLQFPAEDLGFRYSEGAIIRDNNLLGGRE 540

Query: 561 EPTGRRRQYVPSADPGSRLPHMNVRTLASEDMISTLDLVSGDKVEFLLIIAPLPESYHLA 620
           EPTGRRRQY+PSADPGSRLPHMNVR LASE++ISTLDLVSGDK+EFLLIIAP  ESY LA
Sbjct: 541 EPTGRRRQYLPSADPGSRLPHMNVRVLASEEIISTLDLVSGDKIEFLLIIAPRSESYRLA 600

Query: 621 RSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELTPWENYMDVQEIRQSSTSPSWWDV 680
            +ALK+A EEFKTSVKVC+LWSA+  ++ES S+ +LTPWENY++VQEIRQS TSPSWWDV
Sbjct: 601 HAALKVA-EEFKTSVKVCILWSANTTKIESSSKDQLTPWENYIEVQEIRQSITSPSWWDV 660

Query: 681 CRMTDNGAILVRPDEHIAWRVKSGVSGDLNTQMKRVFTALLK 722
           C+MTD GAILVRPDEHIAWRVKSG+SGD NT++  VFT LLK
Sbjct: 661 CKMTDKGAILVRPDEHIAWRVKSGISGDPNTELIGVFTTLLK 701

BLAST of MC09g1852 vs. TAIR 10
Match: AT1G24340.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 929.5 bits (2401), Expect = 1.7e-270
Identity = 452/709 (63.75%), Postives = 563/709 (79.41%), Query Frame = 0

Query: 21  MGLLGFVNRFNGLRKFDAKLRALPLGLVQSRGLSNSKIFHGGEDTMVPVLIVGAGPVGLV 80
           M +LG + R   +   ++++R  P+   Q + LS++ +F+G +   +PVLIVGAGPVGLV
Sbjct: 1   MAILGLIKRVTRITVNNSRVRVYPVRYFQRKDLSSTNLFNGEDAAKLPVLIVGAGPVGLV 60

Query: 81  LAILLTKLGVKCAVVEKNRSFSNHPQAHFINNRSMEVFRKLHGLAEEIQLCQPPVDSWRK 140
           L+ILLTKLGVKCAVV+K  SFS HPQAHFINNRSME+FR+L GLAEEI+  QPPVD WRK
Sbjct: 61  LSILLTKLGVKCAVVDKATSFSKHPQAHFINNRSMEIFRELDGLAEEIERSQPPVDLWRK 120

Query: 141 FIYCTSLNGAILGSVDHMQPQDFGNIISPVSVAHFSQYKLNRLLLKKLQNLGFQV---CS 200
           FIYCTSL+G+ LG+VDHMQPQDF  ++SP SVAHFSQYKL  LLLK+L++LGF V     
Sbjct: 121 FIYCTSLSGSTLGTVDHMQPQDFEKVVSPASVAHFSQYKLTNLLLKRLEDLGFHVRGSKE 180

Query: 201 PDSLEDTCIVREKQILLGHECVSIDATDDSVTTTASYLKEGKHTERRNICSNILVGTDGA 260
            D LE   +V  +QIL+GHECV IDA  DS+T T S+LK GKH  +RNI  ++LVG DGA
Sbjct: 181 SDGLEADSVV-ARQILMGHECVGIDANKDSITATVSFLKGGKHM-KRNIQCSLLVGADGA 240

Query: 261 GSTVRRLVGIEIKGENDLQKLVSVHFFSRELGEYLLNERPGMLYFIFNTEAIGVLVAHDL 320
           GS VR+L  IE++GE DLQKLVSVHF SRELGEYL++ RPGML+FIFNT+ IGVLVAHDL
Sbjct: 241 GSAVRKLTVIEMRGERDLQKLVSVHFMSRELGEYLISNRPGMLFFIFNTDGIGVLVAHDL 300

Query: 321 KQGEFILQVPFYPPQQSIEDFSPKMCEELIFKLVGLNLCDIDVQDVKPWIMHAEVAEKFI 380
            QGEF+LQ+P+YPPQQS+ DFSP+MC+ LIF LVG  L D+DV D+KPW+MHAEVAEKF+
Sbjct: 301 LQGEFVLQIPYYPPQQSLSDFSPEMCKMLIFNLVGHELSDLDVADIKPWVMHAEVAEKFM 360

Query: 381 CCHNRVLLAGDAAHRFPPAGGFGMNTGIQDVHNLAWKLAAVLQDIASPSILNTYEMERRP 440
           CC NRV+LAGDAAHRFPPAGGFGMNTGIQD HNLAWK+AA++Q  A+ SIL TYE ERRP
Sbjct: 361 CCENRVILAGDAAHRFPPAGGFGMNTGIQDAHNLAWKIAALVQGSANSSILKTYETERRP 420

Query: 441 IAQFNTALSVKNFKAAMEVPAALGLDPRIANSVHQAVNHGLGSVLPSSLQSSVLDGIFKI 500
           IA  NT+LSV+NF+AAM VP+ALGLDP +ANSVH+ +N  +GS+LP+ LQ ++LD +F +
Sbjct: 421 IALSNTSLSVQNFRAAMSVPSALGLDPTVANSVHRFINKTVGSILPTGLQKAILDNVFAL 480

Query: 501 GRMQLSESLLNDKNPIGSSRLAKLRQIFDEGKSLQLQFPAEDLGFRYSEGALIPDNTLLS 560
           GR QLSESLLN+ NP+G+ RL++L+ IF+ GKSLQLQFPAEDLGFRY EGA++PDN   +
Sbjct: 481 GRAQLSESLLNESNPLGNQRLSRLKSIFEGGKSLQLQFPAEDLGFRYLEGAIVPDNESEA 540

Query: 561 G-PEEPTGRRRQYVPSADPGSRLPHMNVRTLAS---EDMISTLDLVSGDKVEFLLIIAPL 620
           G PE P+GRRR YVP A+PGSRLPHM V+ L+    E ++STLDLVS +KVEFLLII+PL
Sbjct: 541 GDPEVPSGRRRDYVPCAEPGSRLPHMYVKILSDSTREVIVSTLDLVSTEKVEFLLIISPL 600

Query: 621 PESYHLARSALKIAAEEFKTSVKVCVLWSADIPRVESRSQQELTPWENYMDVQEI-RQSS 680
            ESY LA +  K+ A+EF  SVKVCV+W +    +E +S   L PWENY+DV E+ +Q+ 
Sbjct: 601 QESYELAHATFKV-AKEFMASVKVCVVWPSSDDGLERKSNSALAPWENYVDVMEVKKQNG 660

Query: 681 TSPSWWDVCRMTDNGAILVRPDEHIAWRVKSGVSGDLNTQMKRVFTALL 722
              SWW +C+M++ G+ILVRPD+HIAWR KSG++ D    M+ VFT +L
Sbjct: 661 EGTSWWSICKMSERGSILVRPDQHIAWRAKSGITLDPTLHMRDVFTIIL 706