MC09g1848 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g1848
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionDNA polymerase alpha subunit B
LocationMC09: 23429332 .. 23435124 (+)
RNA-Seq ExpressionMC09g1848
SyntenyMC09g1848
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGAATTGATCGTTTTTTCTCATGAAAAACCGAACCGACTTACGCTACTACTATAAGTTACTGAAGGCTCTCTTTTCCCTCCAATTTCTGGCAACTCAAAAGAGAAACCGCGGGATCTTTTCAAACCCTAAAATACTGCGATTAGAGGCACTGCCATGGAAGAAGAGATCGGAGCAGAGTTCAAGAGGAGTGGTTTCACGTTCGATGAAGAGGAAGAGATTCTCAAGAAATGTAAGCTCAGCTTGTCTTTTTTGTTTGTTTTATGTCAACGGTTGGATACTAATGAGGCGTGGCGTGGCGATTCTTCCATCTTCTGCATTAGGTCTCACGTTCTGTGTTAATTACAATCTAAAGCCTTCGGATATAGTTTCTAGCTGGGAAGTTTACTATCTCAACAGGTGACTGCTTCTTCTGAACTATTTTCATATCTCCGATGGTCCTACAATTTAACATCTACTTGTCTAATTATCACTTAGAACTTTTCTGAGGCCTTTGTTTTTTTCCCCCAAGGGCAGCAGTAGAAAGCAACTTGATTTTGTGATATTATTTAGCTCCGAAGCTCGAATTCTGGGTTTTGTCTTTATTTTGTGAATGTTTTTCCATTTCTGAAGAGTTCGAGGATAATTGTTTCTCGGTTACAAGCTAATTGAATGTCTTGGACAGGCAATTAGATGGGTCAGTGGTACAGATTTCACAGATGGATGGATTTTTACAACATTTACAAAATGAGCAAAAACAAGCAGTCATTAAAGGGGAACCGGATCTTCATATTTATTCGAGCAAAGATGTGGACATGTATGGATTATTTTCAATATTTTCTTATTTTTCTGCTTTACGTAATAAGCAAGCACAAATTCCACACAACGTAGAATGATATTGATCTTTTGCTTTTCACCATCTATAGCTCACTATCCTATAATTGTGGTGTCAATATACGCACATGGTTTCTTTAGCAATTTTATGAAGTATGACGTACTAGTATGGCGATATGTGGCAATGCTTTAAAAGGTTTAAGGCGACCTCGAGACTCTTTCTCACGAGAGGCAAGACATAAGTCTTGAGGCATTCTGTTAGGTACCTTTAAGTTCGGTAAATTGCCTATTTGTACCAGGTTTGTAAATCTTTTCAGTACTTGATGAAATAACTCTTTCACAATTTAGTGAAAGATTTCAAGTGACACCATCAAACCCAAATAATAACTGTTCATTAATAATACATATAATATAACTAAGTTGGTACTTGTCTCTTGTTGAAATTTTAGTCGTGTTCCCTATTCGGCTGACAAATATCTCTAAGTATTACCGTAAGAAAGTAATAAAATACAATCTGTTCAGGATTTTGAATGACGAGGATGAAGATACAAACGAAGACATTCTTAAAACTCCAAATGGGAAATCTCAGAGCCTCCACAAGGAGCGATTTGACTCAACACCTTACACAAATGAAGGCTTGTTCTCTTCGGGGAAGCCTTCAAAGCATGTGACACCCTTCGGCCAAAGAAGAGAAAAATTTGTTGTGAGGTTCAGCATTAATGGCGTGCCTGATAACGAGACTCCTGAAAAGGAAGATAATTGCGAGAATGATGAGGATGCAATCATCAAGAAGGTTCAACCCCTACAAGGATGCTCCTTGACAGTCCTTGGATCAGGATCTGGACTTGAAGACAATTTCAGGTTCATGCATGACAAGGTTGAAGATAGGGTATTCACTATTTTTCATAGCATTGAATTTGATTCAATGGCACGATCACGACTTTAGTATATGTTCTAAAATAATTTAGTTTCTGATTTTATAGTCTAATGCTCTTGATAACCGAATCATGAAGTATGCCAAAGCTCTTTCTGCATCTGGGCAATATGAGGAAGCAGTGGACCCTACAGCGGCTTCGCAGGTAGTTTAATTTCATTATTTCACTCTTGCCCGATTTCTCTCTGAATTTAAGTGCATGTAAAATTTTTATTGAGAGCTGGGTAGCTTAGGTTTTAGGATTTATTCTACAAATCCAATCGAGGTCATCTAGCTGTTCGTGTTTGAATTTTTATCCTACAATTTTATGACTGCAAATCACAATACACTTTTTCTGAGTAAATGCTTAGCTTGCTTGGTGATACCACAAGTCTAAACGTGTGATCCCGTGTCAGAAAAGCATGTTTGCAGTAGGCATGATCTGTTGTGATGGTGAAGGACACTTAAACGATAAGTCCACCTTGTTACAATGCAGGTATTATTATATATTGTTTATTTTCCTGCTGTTTAGGAGCACTACTTTTCTATTATCGCTACTGTTTCCATTTTGGAAGTAAACAATACAAATACTATATTACTGGTAGTTATGTCATCTATGATCTACTAAACTTCGAGATTTGTTTTTTCTTATTTAGTTTTGAGCATTCAGGGGGGCAGCGAGTTCGTCTAGAATTACAAAACTTAAACCAGTTTTCAATCTTTCCTGGCCAGGTACGGACTGCAATGTAAATACTACTTTTAATAGCATCATCATCCGACATACATACCTACCTACAAAAATTGAATTCATGCAGGTAGTAGGTATTGAAGGGCATAATCCTAGTGGACACTGCTTGATTGCATCCAAACTTGTAGATTATTTTCCTTTGTCTGCCACTGTTGACCCCATTCTACCTCCAGCAAAGAAGAGAGCTTTGAACCATGATATCCAGCCAATGGATCAATCTTCTACACAGTCAAAATTATCAATGGTGTGTATTTTTGTAAATTCCTGATGTTTTTTCAGTCACCTTTTGTACCAGTCATCGTTGGATCATGTATCCTAACTCTTGTATCATATCTCTAGTTAGTAGCACATTAGAACAGGTTAATATTCCTGATCTATTAACATGTTTAATACGCACTTGAAATATTTTTAAACAGATTATTGCATCAGGCCCCTATACAACAACAGACAACTTATTGTTTGAGCCGTTGACAGAGTTATTAGCATATGCAAGAAGGAAGCTTCCTCAGCTACTAATATTGGTAAATGCTCATTCAGATCTTTTGTTTTTATGAAATGACCTGTCATTGTTGTCAGAAGTTCTTGTCATTGACGTTTTATGTTGTTTTAGCTTGGGCCATTTGTTGATTCTGAGCATTCTGATATCAAGAGAGGAATTGTAGAGATGAGCTTGGAGGAACTTTTTAACTTTGAAATTCTCAGAAGGGTAATAACTAACATGGTGTGCTTGACTTTGAAATAATTTATACGTTCCTTTTTTTTTCTTTTAATTTTGGCTTCATCTTGTTCAGGTGCAAGATTATGCAGAACACATGGGTCCTGATGCACGTGTACTGTTGATGCCATCTGTAAGGGATGCTAATCATGCCGAAGTTTTCCCTCAGGTATAAATTGTTGAGGCAGATCTATCAGCTTTTAAATGATTAAATTATTAAAGATCACCTTGTTCTGCAGCCTGCTTTTGACATTCCATCTGAACTTAAACTTCAGGTAATTTATTCGTAGACTTTGAGTTACTTCTAAATTTTATGGAGCTTCATTATCTTGAGTTATGCGTACTGTTGAAACTATGTTGCTTTGGTCCAGATTTCCTGTCTCCCAAACCCAGGCATTCTGGAAGCAAATCAGGTAAAACATGGATACGTCTTTGTGTTTCAAACAAGTTTTCTACGAATTTCTTATTTTGATCAAGATTTTCCTATGTCAGAAACTTAGAAGTTTGAAGTTCCCCATCACTGGTTTTCAATTAGTAGCTGGCTGTTTTTAATACGAAAAGAAAGAAAAATGGGAAAGAACAAGAGCTGGATGCTACATTGTAAATAAACACCAACAGATCCTTTCGAAAAAACCTTTCGAAAAAATAAACACCAACAGATAACAAAGCAAAACAAAAAAAGATTGATCCAACTAAGACCATCATCGCTGTTAGTCTGTCCATGATGTATGCGCCTGTGCTGGAGTAGAAATTTCAATAACAGACCATTAGTATTCTATTTCTTATAAATGAATAATTACATTTAGTTCCGGGTATCGTGCACCTGCACTTGCTGAGTTTATACCATGAGATAGTTTTTAAATTCTCATCAGAAAGGAAAAATGGAAAAAAAGTTAATAGTATTTTATTACCCTTTTGTAGAAAACAAATATAGACGTGGCGCCTCGTTTATCTCTTCTTGCCCTTCTCTTTTGGAATTCTGACAAAGTAAAAACAATCCAATGCATAATGGGAGAATGGAAGATCCTTTTGATTTCCTAACTGGATTCCCTTCTGCAGTTGATTTGGTTATTATCCTTTTGTTATCTTTTAATTGGAGGGAGGAGGGATCGGAAAGAAAGACTGATAGGAATGCCACGTGATATCTGCTTTTATTAGTTTTAAACAGAGTTCTAAAATATAGGCAAATATAATAAACCATGTTTCGGTTTGTGCAAAGTCTGTTGGCTAAGCATCTTTTAGCTGGCTATAGGGATATAATCAAATATTGACGTCTTAATTGTTTGAAACCTAGACTACCGAGCTTTCTTAGTTATTACATGCACGCAGTGGCGCAATAATAGTAAAAGTTCAAGAATACATAACCGACGTCTCATGTATATTTCCATCCATAATGGTTTCAGGTAAAGATAGCCTGCTGCACGACGGATATTCTCAGACACCTTAGTGGAGAAGAACTGTCGAGAAACCTAACCAAGGGATCAACCAATGACCGTCTGAGCACACTTGCAAGTCATATACTTCGCCAGCGAAGGCATGTTTTATGTTGGACACAAGAATGTTGGATATCCAACGTATTAATATAGTGTTCTCTTTATCTTACGGTTCTCTTGTTTCACTTGTTCGCAATGCAAACCAGCTTTTATCCTCTATACCCTCCAGCCGAAGGAGTTCCACTGGACTTTTCACTTGCCGCAGAAGCTCTTGACATCTCTGTAATACCCGATATTCTTATTCTTCCATCCGACATGAAACACTTCGTGAAGGTAAATGACTGTCCACTTTATTTTATTACTGTACATTTTACGTAGTATTGTCAGTTTTGTCTTCTTATCTTGCATTAATTTGTTCATTGTCAATACTAAGTCAATGATTAAAGTAAAAGGGTGGCAATTTTATTACACGTGTTATACATTCTAGATCAATGACTGGAGTTTAACCTTCTTATTTCTGCCCGTAAGCAGATAAAACGGACATGTTATTAAGAGCTTCAATGACTTCATTCTGTATCAGGTTATATCCCTTGGTGAAGGAGGATCAGAGGAAGAGCGAGCGAGCTGCGTATGTGTGAATCCCGGAAGACTGGCCAAGGGCGAAGGAGGGGGCACTTTTGTAGAGCTTAGTTACTTTGGTGCGCCGGACCAGATAAATGCTGCAGTTGTCAGCATATGAAAACGGAAGAGCAGAGCGTTTGTCTTTCGTCATTATTTTCAAATATGAAAACCCATTGTATTGAAATTCACTCACTGTACCATATCTTAGAAACGAGAGCTGATCAAAATATCAGTAACTTTTTTCAAACAATGAACTAATTTGTTTATAAGTATATCAATTGCATATAAGCATCATGTAGAAATTTTTAAGATTATCATAAACAAAAGCATCCACCTACTCTACTGCTTTCTCTACTCTAAACTAAATGGATTTTAGTCCAAAGCTAAAATGTATTCACACTACTTTGCCATGTCTTCTCTGGCGAAACCAGAGAGCCCAGTGGTTTCCAATCCACAATATACTAACCAATATAGCAGTTGCAATTACACCCATCCACGAAATCCATGCCCACTTGGGAATCACATTCTCAGCCGCACCACTAACATAAAAT

mRNA sequence

CTGAATTGATCGTTTTTTCTCATGAAAAACCGAACCGACTTACGCTACTACTATAAGTTACTGAAGGCTCTCTTTTCCCTCCAATTTCTGGCAACTCAAAAGAGAAACCGCGGGATCTTTTCAAACCCTAAAATACTGCGATTAGAGGCACTGCCATGGAAGAAGAGATCGGAGCAGAGTTCAAGAGGAGTGGTTTCACGTTCGATGAAGAGGAAGAGATTCTCAAGAAATGTCTCACGTTCTGTGTTAATTACAATCTAAAGCCTTCGGATATAGTTTCTAGCTGGGAAGTTTACTATCTCAACAGGCAATTAGATGGGTCAGTGGTACAGATTTCACAGATGGATGGATTTTTACAACATTTACAAAATGAGCAAAAACAAGCAGTCATTAAAGGGGAACCGGATCTTCATATTTATTCGAGCAAAGATGTGGACATGATTTTGAATGACGAGGATGAAGATACAAACGAAGACATTCTTAAAACTCCAAATGGGAAATCTCAGAGCCTCCACAAGGAGCGATTTGACTCAACACCTTACACAAATGAAGGCTTGTTCTCTTCGGGGAAGCCTTCAAAGCATGTGACACCCTTCGGCCAAAGAAGAGAAAAATTTGTTGTGAGGTTCAGCATTAATGGCGTGCCTGATAACGAGACTCCTGAAAAGGAAGATAATTGCGAGAATGATGAGGATGCAATCATCAAGAAGGTTCAACCCCTACAAGGATGCTCCTTGACAGTCCTTGGATCAGGATCTGGACTTGAAGACAATTTCAGGTTCATGCATGACAAGGTTGAAGATAGGTCTAATGCTCTTGATAACCGAATCATGAAGTATGCCAAAGCTCTTTCTGCATCTGGGCAATATGAGGAAGCAGTGGACCCTACAGCGGCTTCGCAGAAAAGCATGTTTGCAGTAGGCATGATCTGTTGTGATGGTGAAGGACACTTAAACGATAAGTCCACCTTGTTACAATGCAGTTTTGAGCATTCAGGGGGGCAGCGAGTTCGTCTAGAATTACAAAACTTAAACCAGTTTTCAATCTTTCCTGGCCAGGTAGTAGGTATTGAAGGGCATAATCCTAGTGGACACTGCTTGATTGCATCCAAACTTGTAGATTATTTTCCTTTGTCTGCCACTGTTGACCCCATTCTACCTCCAGCAAAGAAGAGAGCTTTGAACCATGATATCCAGCCAATGGATCAATCTTCTACACAGTCAAAATTATCAATGATTATTGCATCAGGCCCCTATACAACAACAGACAACTTATTGTTTGAGCCGTTGACAGAGTTATTAGCATATGCAAGAAGGAAGCTTCCTCAGCTACTAATATTGCTTGGGCCATTTGTTGATTCTGAGCATTCTGATATCAAGAGAGGAATTGTAGAGATGAGCTTGGAGGAACTTTTTAACTTTGAAATTCTCAGAAGGGTGCAAGATTATGCAGAACACATGGGTCCTGATGCACGTGTACTGTTGATGCCATCTGTAAGGGATGCTAATCATGCCGAAGTTTTCCCTCAGCCTGCTTTTGACATTCCATCTGAACTTAAACTTCAGATTTCCTGTCTCCCAAACCCAGGCATTCTGGAAGCAAATCAGGTAAAGATAGCCTGCTGCACGACGGATATTCTCAGACACCTTAGTGGAGAAGAACTGTCGAGAAACCTAACCAAGGGATCAACCAATGACCGTCTGAGCACACTTGCAAGTCATATACTTCGCCAGCGAAGGCATGTTTTATGTTGGACACAAGAATGTTGGATATCCAACGTATTAATATACTTTTATCCTCTATACCCTCCAGCCGAAGGAGTTCCACTGGACTTTTCACTTGCCGCAGAAGCTCTTGACATCTCTGTAATACCCGATATTCTTATTCTTCCATCCGACATGAAACACTTCGTGAAGGTTATATCCCTTGGTGAAGGAGGATCAGAGGAAGAGCGAGCGAGCTGCGTATGTGTGAATCCCGGAAGACTGGCCAAGGGCGAAGGAGGGGGCACTTTTGTAGAGCTTAGTTACTTTGGTGCGCCGGACCAGATAAATGCTGCAGTTGTCAGCATATGAAAACGGAAGAGCAGAGCGTTTGTCTTTCGTCATTATTTTCAAATATGAAAACCCATTGTATTGAAATTCACTCACTGTACCATATCTTAGAAACGAGAGCTGATCAAAATATCAGTAACTTTTTTCAAACAATGAACTAATTTGTTTATAAGTATATCAATTGCATATAAGCATCATGTAGAAATTTTTAAGATTATCATAAACAAAAGCATCCACCTACTCTACTGCTTTCTCTACTCTAAACTAAATGGATTTTAGTCCAAAGCTAAAATGTATTCACACTACTTTGCCATGTCTTCTCTGGCGAAACCAGAGAGCCCAGTGGTTTCCAATCCACAATATACTAACCAATATAGCAGTTGCAATTACACCCATCCACGAAATCCATGCCCACTTGGGAATCACATTCTCAGCCGCACCACTAACATAAAAT

Coding sequence (CDS)

ATGGAAGAAGAGATCGGAGCAGAGTTCAAGAGGAGTGGTTTCACGTTCGATGAAGAGGAAGAGATTCTCAAGAAATGTCTCACGTTCTGTGTTAATTACAATCTAAAGCCTTCGGATATAGTTTCTAGCTGGGAAGTTTACTATCTCAACAGGCAATTAGATGGGTCAGTGGTACAGATTTCACAGATGGATGGATTTTTACAACATTTACAAAATGAGCAAAAACAAGCAGTCATTAAAGGGGAACCGGATCTTCATATTTATTCGAGCAAAGATGTGGACATGATTTTGAATGACGAGGATGAAGATACAAACGAAGACATTCTTAAAACTCCAAATGGGAAATCTCAGAGCCTCCACAAGGAGCGATTTGACTCAACACCTTACACAAATGAAGGCTTGTTCTCTTCGGGGAAGCCTTCAAAGCATGTGACACCCTTCGGCCAAAGAAGAGAAAAATTTGTTGTGAGGTTCAGCATTAATGGCGTGCCTGATAACGAGACTCCTGAAAAGGAAGATAATTGCGAGAATGATGAGGATGCAATCATCAAGAAGGTTCAACCCCTACAAGGATGCTCCTTGACAGTCCTTGGATCAGGATCTGGACTTGAAGACAATTTCAGGTTCATGCATGACAAGGTTGAAGATAGGTCTAATGCTCTTGATAACCGAATCATGAAGTATGCCAAAGCTCTTTCTGCATCTGGGCAATATGAGGAAGCAGTGGACCCTACAGCGGCTTCGCAGAAAAGCATGTTTGCAGTAGGCATGATCTGTTGTGATGGTGAAGGACACTTAAACGATAAGTCCACCTTGTTACAATGCAGTTTTGAGCATTCAGGGGGGCAGCGAGTTCGTCTAGAATTACAAAACTTAAACCAGTTTTCAATCTTTCCTGGCCAGGTAGTAGGTATTGAAGGGCATAATCCTAGTGGACACTGCTTGATTGCATCCAAACTTGTAGATTATTTTCCTTTGTCTGCCACTGTTGACCCCATTCTACCTCCAGCAAAGAAGAGAGCTTTGAACCATGATATCCAGCCAATGGATCAATCTTCTACACAGTCAAAATTATCAATGATTATTGCATCAGGCCCCTATACAACAACAGACAACTTATTGTTTGAGCCGTTGACAGAGTTATTAGCATATGCAAGAAGGAAGCTTCCTCAGCTACTAATATTGCTTGGGCCATTTGTTGATTCTGAGCATTCTGATATCAAGAGAGGAATTGTAGAGATGAGCTTGGAGGAACTTTTTAACTTTGAAATTCTCAGAAGGGTGCAAGATTATGCAGAACACATGGGTCCTGATGCACGTGTACTGTTGATGCCATCTGTAAGGGATGCTAATCATGCCGAAGTTTTCCCTCAGCCTGCTTTTGACATTCCATCTGAACTTAAACTTCAGATTTCCTGTCTCCCAAACCCAGGCATTCTGGAAGCAAATCAGGTAAAGATAGCCTGCTGCACGACGGATATTCTCAGACACCTTAGTGGAGAAGAACTGTCGAGAAACCTAACCAAGGGATCAACCAATGACCGTCTGAGCACACTTGCAAGTCATATACTTCGCCAGCGAAGGCATGTTTTATGTTGGACACAAGAATGTTGGATATCCAACGTATTAATATACTTTTATCCTCTATACCCTCCAGCCGAAGGAGTTCCACTGGACTTTTCACTTGCCGCAGAAGCTCTTGACATCTCTGTAATACCCGATATTCTTATTCTTCCATCCGACATGAAACACTTCGTGAAGGTTATATCCCTTGGTGAAGGAGGATCAGAGGAAGAGCGAGCGAGCTGCGTATGTGTGAATCCCGGAAGACTGGCCAAGGGCGAAGGAGGGGGCACTTTTGTAGAGCTTAGTTACTTTGGTGCGCCGGACCAGATAAATGCTGCAGTTGTCAGCATATGA

Protein sequence

MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQISQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLHKERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDEDAIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQYEEAVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPGQVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSMIIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELFNFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGILEANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVLCWTQECWISNVLIYFYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEERASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI
Homology
BLAST of MC09g1848 vs. ExPASy Swiss-Prot
Match: Q14181 (DNA polymerase alpha subunit B OS=Homo sapiens OX=9606 GN=POLA2 PE=1 SV=2)

HSP 1 Score: 245.0 bits (624), Expect = 2.3e-63
Identity = 180/632 (28.48%), Postives = 298/632 (47.15%), Query Frame = 0

Query: 14  FTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI--SQMDGFLQHLQ 73
           F  D EE +++K +  CV Y      +V     +  +    G   +I  S    FL    
Sbjct: 15  FGLDCEEALIEKLVELCVQYGQNEEGMVGELIAFCTSTHKVGLTSEILNSFEHEFLSKRL 74

Query: 74  NEQKQAVIKGEPDLHIYSSKDV----DMILNDEDEDTNEDILKTPNGKSQSLHKERFDST 133
           ++ + +  K   D     ++D+    ++I  +E+E+   +   TP+  SQ    +R  ST
Sbjct: 75  SKARHSTCK---DSGHAGARDIVSIQELIEVEEEEEILLNSYTTPSKGSQ----KRAIST 134

Query: 134 PYT--NEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDEDAIIKK 193
           P T   +   S+  P + ++P              +    + TP ++ N  ++   ++  
Sbjct: 135 PETPLTKRSVSTRSPHQLLSP--------------SSFSPSATPSQKYNSRSNRGEVVTS 194

Query: 194 VQPLQG-----------CSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSA 253
               QG            SL VLG    L  +++ M  K+ D    L  +I +    L  
Sbjct: 195 FGLAQGVSWSGRGGAGNISLKVLGCPEALTGSYKSMFQKLPDIREVLTCKIEELGSELKE 254

Query: 254 SGQYEEAVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQ 313
             + E      A +Q+ +  +G I CD  G LN+KS +L+   EHS G ++ ++L  L +
Sbjct: 255 HYKIEAFTPLLAPAQEPVTLLGQIGCDSNGKLNNKSVILEGDREHSSGAQIPVDLSELKE 314

Query: 314 FSIFPGQVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSST 373
           +S+FPGQVV +EG N +G  L+A+KL +  PL                    QP ++ + 
Sbjct: 315 YSLFPGQVVIMEGINTTGRKLVATKLYEGVPLPF-----------------YQPTEEDAD 374

Query: 374 QSKLSMIIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEM 433
             +  +++A GPYTT+D++ ++PL +L+A      P + IL GPF+D++H  ++  ++  
Sbjct: 375 FEQSMVLVACGPYTTSDSITYDPLLDLIAVINHDRPDVCILFGPFLDAKHEQVENCLLTS 434

Query: 434 SLEELFNFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAF---DIPSELKLQI 493
             E++F  + LR + +     G  + ++ +PS+RD +H  V+PQP F   D+  E K Q+
Sbjct: 435 PFEDIFK-QCLRTIIEGTRSSG--SHLVFVPSLRDVHHEPVYPQPPFSYSDLSREDKKQV 494

Query: 494 SCLPNPGILEANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVL 553
             +  P  L  N V     +TD+L HL  EE+S   +   T+DR S +  HIL QR    
Sbjct: 495 QFVSEPCSLSINGVIFGLTSTDLLFHLGAEEIS---SSSGTSDRFSRILKHILTQRS--- 554

Query: 554 CWTQECWISNVLIYFYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISL 613
                         +YPLYPP E + +D+        + V PD+LI+PS++++FVK +  
Sbjct: 555 --------------YYPLYPPQEDMAIDYESFYVYAQLPVTPDVLIIPSELRYFVKDV-- 574

Query: 614 GEGGSEEERASCVCVNPGRLAKGEGGGTFVEL 624
                      CVCVNPGRL KG+ GGTF  L
Sbjct: 615 ---------LGCVCVNPGRLTKGQVGGTFARL 574

BLAST of MC09g1848 vs. ExPASy Swiss-Prot
Match: P33611 (DNA polymerase alpha subunit B OS=Mus musculus OX=10090 GN=Pola2 PE=1 SV=2)

HSP 1 Score: 227.6 bits (579), Expect = 3.9e-58
Identity = 169/629 (26.87%), Postives = 287/629 (45.63%), Query Frame = 0

Query: 14  FTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQISQMDGFLQHLQNE 73
           F  D E+ +L+K    CV Y      +VS  E+         + + +  ++ F   + N+
Sbjct: 15  FGLDYEDSLLEKLAELCVLYRQTEDGMVS--ELIAFCTSAGKTCLTVDILNSFEYEVLNK 74

Query: 74  Q-KQAVIKGEPDLHIYSSKDV----DMILNDEDEDTNEDILKTPNGKSQSLHKERFDSTP 133
           +  +A      D     ++D+    ++I  +E+E+T      TP+ K           TP
Sbjct: 75  KLSKAWHSASKDSGHAGTRDIVSIQELIEAEEEEETLLSSYTTPS-KGPLKRVSSTPETP 134

Query: 134 YTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDEDAIIKKVQP 193
            T   + ++  P + ++P              +    + TP ++     +   ++     
Sbjct: 135 LTKRSV-AARSPRQLLSP--------------SSFSPSATPSQKYTSRTNRGEVVTTFGS 194

Query: 194 LQG-----------CSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQ 253
            QG            SL V+G    L  +++ M  ++      L ++I +    L    +
Sbjct: 195 AQGLSWSGRGGSGSVSLKVVGDPEPLTGSYKAMFQQLMGVREVLTSKIEELGSELKEHHK 254

Query: 254 YEEAVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSI 313
            E        +Q+ +  +G I CD  G LN KS +L+   EHS G ++ ++L  L ++S+
Sbjct: 255 IEAFTPLLVPAQEPVILLGQIGCDSNGKLNSKSVILEGDQEHSYGAQIPVDLSELKEYSL 314

Query: 314 FPGQVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSK 373
           FPGQVV +EG N +G  L A+KL +  PL                    QP ++     +
Sbjct: 315 FPGQVVIMEGFNTTGRRLTATKLYEGVPLPF-----------------YQPTEEEGASEQ 374

Query: 374 LSMIIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLE 433
             +++A GPYTT+D++ ++PL +L+A   R  P + IL GPF+D++H  ++   +    E
Sbjct: 375 TMVVVACGPYTTSDSITYDPLLDLIAIINRDQPDVCILFGPFLDAKHEQVENCKLTSPFE 434

Query: 434 ELFNFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAF---DIPSELKLQISCL 493
           ++F  + LR V +     G  + ++ +PS+RD +H  V+PQP F   ++  E K ++  +
Sbjct: 435 DVFK-QCLRTVIEGTRSSG--SHLVFVPSLRDVHHEPVYPQPPFTFSELSREDKKRVQFV 494

Query: 494 PNPGILEANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVLCWT 553
             P  L  N V     +TD+L H+  EE+    +   T+DR S +  HIL QR       
Sbjct: 495 SEPCSLSINGVMFGLTSTDLLFHIGAEEI---FSSSGTSDRFSRVLKHILTQRS------ 554

Query: 554 QECWISNVLIYFYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEG 613
                      +YPLYPP E + +D+        + V PD+ I+PS++++FVK I     
Sbjct: 555 -----------YYPLYPPHEDMAIDYENFYTYAQLPVTPDVFIVPSELRYFVKDI----- 574

Query: 614 GSEEERASCVCVNPGRLAKGEGGGTFVEL 624
                   CVCVNPGRL KG+ GGTF  L
Sbjct: 615 ------FGCVCVNPGRLTKGQVGGTFGRL 574

BLAST of MC09g1848 vs. ExPASy Swiss-Prot
Match: O89043 (DNA polymerase alpha subunit B OS=Rattus norvegicus OX=10116 GN=Pola2 PE=1 SV=2)

HSP 1 Score: 227.6 bits (579), Expect = 3.9e-58
Identity = 171/632 (27.06%), Postives = 287/632 (45.41%), Query Frame = 0

Query: 14  FTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQISQMDGFLQH--LQ 73
           F  D E+ +L+K    C  Y      +VS    +  +    G      ++    +H  L 
Sbjct: 15  FGLDCEDSLLEKLAELCTLYRQTEERMVSELIAFCTSA---GKSCLTGEILSSFEHEVLN 74

Query: 74  NEQKQAVIKGEPDLHIYSSKDV----DMILNDEDEDTNEDILKTPNGKSQSLHKERFDST 133
            +  +A      D     ++D+    ++I  +E+E+T      TP   S+  HK R  ST
Sbjct: 75  KKLSKACHSASKDNRHAGARDIVSIQELIEAEEEEETLLSAYTTP---SKGPHK-RVSST 134

Query: 134 PYT--NEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDEDAIIKK 193
           P T   +   S+  P + ++P              +    + TP ++ +   +   ++  
Sbjct: 135 PETPLTKRSISTRSPHQLLSP--------------SSFSPSATPSQKYSSRTNRGEVVTT 194

Query: 194 VQPLQG-----------CSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSA 253
               QG            SL V+G    L  +++ M  ++ D    L  +I +    L  
Sbjct: 195 FGSAQGVSWSGRGGSGSISLKVMGYPEPLTSSYKTMFQQLPDIREVLTCKIEELGSELKE 254

Query: 254 SGQYEEAVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQ 313
             + E        +Q+ +  +G I CD  G LN KS +L+   EHS G ++ +++  L  
Sbjct: 255 HYKIEAFTPLLVPAQEPVILLGQIGCDSNGRLNSKSVILEGDREHSSGAQIPVDVSELKD 314

Query: 314 FSIFPGQVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSST 373
           +S+FPGQVV +EG N +G  L A+KL +  PL                    QP ++   
Sbjct: 315 YSLFPGQVVIMEGFNTTGRRLTATKLYEGVPLPF-----------------YQPTEEEGD 374

Query: 374 QSKLSMIIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEM 433
             +  +++A GPYTT+D++ ++PL +L++      P + IL GPF+D++H  ++   +  
Sbjct: 375 FEQTMVLVACGPYTTSDSITYDPLLDLISTINHDRPDVCILFGPFLDAKHEQVENCKLTS 434

Query: 434 SLEELFNFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAF---DIPSELKLQI 493
             E++F  + LR V +     G  + ++ +PS+RD +H  V+PQP F   ++P E K ++
Sbjct: 435 PFEDIFK-QCLRTVIEGTRSSG--SHLVFVPSLRDVHHEPVYPQPPFTFSELPREDKKRV 494

Query: 494 SCLPNPGILEANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVL 553
             +  P  L  N V     +TD+L H+  EE+    +   T+DR S +  HIL QR    
Sbjct: 495 QFVSEPCNLSINGVMFGLTSTDLLFHIGAEEI---CSSSGTSDRFSRILKHILTQRS--- 554

Query: 554 CWTQECWISNVLIYFYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISL 613
                         +YPLYPP E + +D+        + V PD+ I+PS++++FVK I  
Sbjct: 555 --------------YYPLYPPHEDMAIDYENFYTYAQLPVTPDVFIVPSELRYFVKDI-- 574

Query: 614 GEGGSEEERASCVCVNPGRLAKGEGGGTFVEL 624
                      CVC+NPGRL KG+ GGTF  L
Sbjct: 615 ---------FGCVCMNPGRLTKGQVGGTFGRL 574

BLAST of MC09g1848 vs. ExPASy Swiss-Prot
Match: Q58D13 (DNA polymerase alpha subunit B OS=Bos taurus OX=9913 GN=POLA2 PE=2 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 3.4e-54
Identity = 158/597 (26.47%), Postives = 281/597 (47.07%), Query Frame = 0

Query: 14  FTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI--SQMDGFLQHLQ 73
           F  + EE +++K +  C+ Y      + S    +  + + D   ++   S    FL    
Sbjct: 15  FGLECEEAVIEKLVELCILYGQNEEGMASELIAFCTSTRKDCFTLETLNSFEHEFLSKRV 74

Query: 74  NEQKQAVIKGEPDLHIYSSKDV----DMILNDEDEDTNEDILKTPNGKSQSLHKERFDST 133
           ++ +    K +  L    ++D+    ++I  +E+E+T  +   TP+  SQ    +R  +T
Sbjct: 75  SKTRHGASK-DKGLRHAGARDIVSIQELIEVEEEEETLLNSYTTPSKGSQ----KRTITT 134

Query: 134 PYT--NEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDEDAIIKK 193
           P T   +   S+  P + ++P              +    + TP ++ +  ++   ++  
Sbjct: 135 PETPLTKRSVSARSPHQLLSP--------------SSFSPSATPPQKYSSRSNRGEVVTS 194

Query: 194 VQPLQG-----------CSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSA 253
               QG            SL VLG    L  ++++M  K+ D    L  +I +    L  
Sbjct: 195 FGSAQGVSWSGRGGASPLSLKVLGHPEPLTGSYKYMFQKLPDIREVLTCKIEELGSELKE 254

Query: 254 SGQYEEAVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQ 313
             + E        +Q+ +  +G I CD  G LN KS +L+   EHS G ++ ++L  L +
Sbjct: 255 HYKIEAFAPILVPAQEPVTLLGQIGCDSNGKLNHKSVILEGDLEHSSGAQIPVDLSELKE 314

Query: 314 FSIFPGQVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSST 373
           +S+FPGQVV +EG N +G  L+A++L +  PL                    QP ++   
Sbjct: 315 YSLFPGQVVVMEGINTTGRKLVATRLYEGVPLPFH-----------------QPDEEDGD 374

Query: 374 QSKLSMIIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEM 433
             +  +++A GPYTT+D++ F+PL +L+    R  P + IL GPF+D++H  ++  ++  
Sbjct: 375 SEQFMVLVACGPYTTSDSITFDPLLDLITIINRDRPDVCILFGPFLDAKHEQVESCLLTS 434

Query: 434 SLEELFNFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAF---DIPSELKLQI 493
           S E++F  + LR + +     G  + ++++PS+RD +H  V+PQP F   D+  E K ++
Sbjct: 435 SFEDVFK-QCLRTIIEGTRSSG--SHLIIVPSLRDVHHEPVYPQPPFSCSDLLREDKKRV 494

Query: 494 SCLPNPGILEANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVL 553
             +  P  L  N V     +TD+L H+  EE+S   +   T+DR S +  HIL QR    
Sbjct: 495 RLVSEPCTLSINGVIFGLTSTDLLFHMGAEEIS---SSSGTSDRFSRILRHILTQRS--- 552

Query: 554 CWTQECWISNVLIYFYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKV 589
                         +YPLYPP E + +D+        + V PD+ I PS++++FVKV
Sbjct: 555 --------------YYPLYPPQEDMAIDYENFYLYAQLPVTPDVFIAPSELRYFVKV 552

BLAST of MC09g1848 vs. ExPASy Swiss-Prot
Match: O74946 (DNA polymerase alpha subunit B OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pol12 PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 4.9e-45
Identity = 139/435 (31.95%), Postives = 212/435 (48.74%), Query Frame = 0

Query: 206 NFRFMHDKVEDRSNALDNRIMKYAKALSASGQY--EEAVDPTAASQKSMFAVGMICCDGE 265
           N+R M+ K+ D S  LD+RI  ++           E+  +P+  +Q+ +  VG I  +  
Sbjct: 157 NYRIMYQKLYDSSEVLDDRIELFSALTCRKYNISDEDLANPSELTQEPVVVVGRIVVEST 216

Query: 266 ---GHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPGQVVGIEGHNPSGHCLIASKL 325
              G LN  S LL+ S     G RVRL++ +L  +SIFPGQ+V ++G NPSG+  IA ++
Sbjct: 217 NLGGRLNQNSILLESSRRLGAGVRVRLKVDDLPSYSIFPGQIVSVKGSNPSGNMFIAKEI 276

Query: 326 VDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSMIIASGPYTTTDNLLFEPLTE 385
           +   PL        P + K+    +      ++    +S+ IASGP++  D+L F PL  
Sbjct: 277 LPIPPLP------FPSSSKQ----EHATFVANTNNQPISIYIASGPWSLRDDLSFSPLKS 336

Query: 386 LLAYARRKLPQLLILLGPFVDSEHSDIKRGIV----EMSLEELFNFEILRRVQDYAEHMG 445
           +++Y  +    L+IL GPF+D  H  I+ G +      SLEELF      RV      + 
Sbjct: 337 MISYVNKNPVDLVILCGPFLDINHILIRTGNITGTSATSLEELFK----ERVTPILSQL- 396

Query: 446 PDARVLLMPSVRD-ANHAEVFPQPAFD-IPSELKLQISCLPNPGILEANQVKIACCTTDI 505
                +L+P + D A+    +PQ AF+ +   L     C PNP +   N V     T DI
Sbjct: 397 -TCPCILIPHINDAASDHPAWPQDAFNRVALGLPSNFKCFPNPCMFSINDVVFGVSTNDI 456

Query: 506 LRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVLCWTQECWISNVLIYFYPLYPPA- 565
           L H S EEL R  + G+     + L SH+L QR                 +FYPL+P   
Sbjct: 457 LLHTSREELFRLPSHGNL---FARLVSHVLHQR-----------------HFYPLFPGGS 516

Query: 566 --EGVPLDFSLAAEALD--ISVIPDILILPSDMKHFVKVISLGEGGSEEERASCVCVNPG 625
             +  P +  +A   L   ++ +PDILILPSD+++FVK +            + V +NPG
Sbjct: 517 LEKCNPSNLDIAHLKLGEFLNTMPDILILPSDLRYFVKNVE-----------NVVSLNPG 544

BLAST of MC09g1848 vs. NCBI nr
Match: XP_022151967.1 (DNA polymerase alpha subunit B isoform X1 [Momordica charantia])

HSP 1 Score: 1222 bits (3162), Expect = 0.0
Identity = 621/639 (97.18%), Postives = 621/639 (97.18%), Query Frame = 0

Query: 1   MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI 60
           MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI
Sbjct: 1   MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI 60

Query: 61  SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH 120
           SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH
Sbjct: 61  SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH 120

Query: 121 KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED 180
           KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED
Sbjct: 121 KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED 180

Query: 181 AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQYEE 240
           AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQYEE
Sbjct: 181 AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQYEE 240

Query: 241 AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG 300
           AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG
Sbjct: 241 AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG 300

Query: 301 QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM 360
           QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM
Sbjct: 301 QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM 360

Query: 361 IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF 420
           IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF
Sbjct: 361 IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF 420

Query: 421 NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL 480
           NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL
Sbjct: 421 NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL 480

Query: 481 EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVLCWTQECWIS 540
           EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQR             
Sbjct: 481 EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRS------------ 540

Query: 541 NVLIYFYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER 600
                FYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER
Sbjct: 541 -----FYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER 600

Query: 601 ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI 639
           ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI
Sbjct: 601 ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI 622

BLAST of MC09g1848 vs. NCBI nr
Match: XP_022151968.1 (DNA polymerase alpha subunit B isoform X2 [Momordica charantia])

HSP 1 Score: 1209 bits (3127), Expect = 0.0
Identity = 617/639 (96.56%), Postives = 617/639 (96.56%), Query Frame = 0

Query: 1   MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI 60
           MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI
Sbjct: 1   MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI 60

Query: 61  SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH 120
           SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH
Sbjct: 61  SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH 120

Query: 121 KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED 180
           KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED
Sbjct: 121 KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED 180

Query: 181 AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQYEE 240
           AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDK    SNALDNRIMKYAKALSASGQYEE
Sbjct: 181 AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDK----SNALDNRIMKYAKALSASGQYEE 240

Query: 241 AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG 300
           AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG
Sbjct: 241 AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG 300

Query: 301 QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM 360
           QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM
Sbjct: 301 QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM 360

Query: 361 IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF 420
           IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF
Sbjct: 361 IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF 420

Query: 421 NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL 480
           NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL
Sbjct: 421 NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL 480

Query: 481 EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVLCWTQECWIS 540
           EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQR             
Sbjct: 481 EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRS------------ 540

Query: 541 NVLIYFYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER 600
                FYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER
Sbjct: 541 -----FYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER 600

Query: 601 ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI 639
           ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI
Sbjct: 601 ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI 618

BLAST of MC09g1848 vs. NCBI nr
Match: KAG7012749.1 (DNA polymerase alpha subunit B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1085 bits (2807), Expect = 0.0
Identity = 544/639 (85.13%), Postives = 582/639 (91.08%), Query Frame = 0

Query: 1   MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI 60
           M++EIG EFKRSGFTFDEEEEIL KC+TFCVNY+LKPSDIVSSWEVYYLNRQLDGS+VQI
Sbjct: 1   MDDEIGEEFKRSGFTFDEEEEILNKCVTFCVNYSLKPSDIVSSWEVYYLNRQLDGSIVQI 60

Query: 61  SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH 120
           +QMDGFLQHLQNEQK+A+IK EPDLH+YSSKDVDMILNDEDED  EDILKTPNGKSQ LH
Sbjct: 61  AQMDGFLQHLQNEQKEAIIKEEPDLHVYSSKDVDMILNDEDEDPKEDILKTPNGKSQGLH 120

Query: 121 KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED 180
           K+RFD TPYTNEGLFSSGKPSKHVTPFGQR EKF VRFSIN VPD ET E EDNCENDED
Sbjct: 121 KDRFDLTPYTNEGLFSSGKPSKHVTPFGQRTEKFAVRFSINNVPDKETAEMEDNCENDED 180

Query: 181 AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQYEE 240
           AIIKKV PLQGCSLTV GSG GLE N RFM+D+VEDR NAL+NRI+K+AKAL ASG YEE
Sbjct: 181 AIIKKVLPLQGCSLTVHGSGFGLEPNCRFMYDRVEDRFNALENRIVKHAKALVASGFYEE 240

Query: 241 AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG 300
            VDPT ASQKSMFAVGMICCDG+GHLNDKST+LQ S EHSGGQRVRLELQNL+QFSIFPG
Sbjct: 241 VVDPTVASQKSMFAVGMICCDGDGHLNDKSTMLQSSVEHSGGQRVRLELQNLSQFSIFPG 300

Query: 301 QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM 360
           QVVGIEGHNPSGHCLIASKLVD FPLSAT+DPILPPAKK AL+HDIQP++Q ST+S+LSM
Sbjct: 301 QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALDHDIQPINQPSTESELSM 360

Query: 361 IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF 420
           IIASGPYTTTDN+LFEPLTELLAYA+RK PQLLILLGPFVDSEHSDIKRG V MS EE+F
Sbjct: 361 IIASGPYTTTDNILFEPLTELLAYAKRKPPQLLILLGPFVDSEHSDIKRGTVAMSFEEIF 420

Query: 421 NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL 480
           NFE+LRRVQDY EHMGPDARVLLMPS+RDANH  VFPQPAFDIPS+L+LQIS LPNPGIL
Sbjct: 421 NFEVLRRVQDYVEHMGPDARVLLMPSIRDANHDFVFPQPAFDIPSDLRLQISSLPNPGIL 480

Query: 481 EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVLCWTQECWIS 540
           EANQ KIACCT DILRHLSGEELSRNLTKG+ NDRLSTLASHILRQRRHV          
Sbjct: 481 EANQAKIACCTMDILRHLSGEELSRNLTKGTANDRLSTLASHILRQRRHV---------- 540

Query: 541 NVLIYFYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER 600
               +FYPLYPPAEGVPLDFSLA +ALDISVIPDILILPSDMKHFVKVISLGEG ++EER
Sbjct: 541 ----FFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGRADEER 600

Query: 601 ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI 639
           A+CVCVNPGRLAKGEGGGTFVEL YFG PD+INAAVVSI
Sbjct: 601 ANCVCVNPGRLAKGEGGGTFVELKYFGTPDKINAAVVSI 625

BLAST of MC09g1848 vs. NCBI nr
Match: XP_023541613.1 (DNA polymerase alpha subunit B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1076 bits (2782), Expect = 0.0
Identity = 541/639 (84.66%), Postives = 578/639 (90.45%), Query Frame = 0

Query: 1   MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI 60
           M++EIG EFKRSGFTFDEEEEILKKC+TFC+NY+LKPSDIVSSWEVYYLNRQLDGS+VQI
Sbjct: 1   MDDEIGEEFKRSGFTFDEEEEILKKCVTFCINYSLKPSDIVSSWEVYYLNRQLDGSIVQI 60

Query: 61  SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH 120
           +QMDGFLQHLQNEQK+A+IK EPDLH+YSSKDVDMILN EDED  EDILKTPNGKSQ LH
Sbjct: 61  AQMDGFLQHLQNEQKEAIIKEEPDLHVYSSKDVDMILNAEDEDPMEDILKTPNGKSQGLH 120

Query: 121 KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED 180
           K+RFD TPYTNEGLFSSGKPSKHVTPFGQR EKF VRFSIN VPD ET E EDNCENDED
Sbjct: 121 KDRFDLTPYTNEGLFSSGKPSKHVTPFGQRTEKFAVRFSINNVPDKETAEMEDNCENDED 180

Query: 181 AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQYEE 240
           AIIKKV PLQGCSLTV GSG GLE N RFM+D+VEDR NAL+NRI+K+AKAL ASG YEE
Sbjct: 181 AIIKKVLPLQGCSLTVHGSGFGLEPNCRFMYDRVEDRFNALENRIVKHAKALVASGLYEE 240

Query: 241 AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG 300
            VDPT ASQKSMFAVGMICCDG+GHLNDKST+LQ S EHSGGQRVRLELQNL QFSIFPG
Sbjct: 241 VVDPTVASQKSMFAVGMICCDGDGHLNDKSTMLQSSVEHSGGQRVRLELQNLGQFSIFPG 300

Query: 301 QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM 360
           QVVGIEGHNPSGHCLIASKLVD FPLSAT+DPILPPAKK AL+HDIQP++Q STQS+LSM
Sbjct: 301 QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALDHDIQPINQPSTQSELSM 360

Query: 361 IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF 420
           IIASGPYTTTDN+LFEPLTELLAYA+RK PQLLILLGPFVDSEHSDIKRG VEMS EE+F
Sbjct: 361 IIASGPYTTTDNILFEPLTELLAYAKRKPPQLLILLGPFVDSEHSDIKRGTVEMSFEEIF 420

Query: 421 NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL 480
           NFE+LRRVQDY EHMGPDARVLLMPS+RDANH  VFPQPAFDIPS+L+LQIS LPNPGIL
Sbjct: 421 NFEVLRRVQDYVEHMGPDARVLLMPSIRDANHDFVFPQPAFDIPSDLRLQISSLPNPGIL 480

Query: 481 EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVLCWTQECWIS 540
           EANQ KIACCT DILRHLSGEELSRNLTKG+ NDRLSTLASHILRQR             
Sbjct: 481 EANQAKIACCTMDILRHLSGEELSRNLTKGTANDRLSTLASHILRQRS------------ 540

Query: 541 NVLIYFYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER 600
                FYPLYPPAEGVPLDFSLA +ALDISVIPDILILPSDMKHFVKVISLGEG ++EER
Sbjct: 541 -----FYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGRADEER 600

Query: 601 ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI 639
           A+CVCVNPGRLAKGEGGGTFVEL YFG P++INAAVVSI
Sbjct: 601 ANCVCVNPGRLAKGEGGGTFVELKYFGTPEKINAAVVSI 622

BLAST of MC09g1848 vs. NCBI nr
Match: XP_022944938.1 (DNA polymerase alpha subunit B [Cucurbita moschata])

HSP 1 Score: 1074 bits (2778), Expect = 0.0
Identity = 540/639 (84.51%), Postives = 577/639 (90.30%), Query Frame = 0

Query: 1   MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI 60
           M++EIG EFKRSGFTFDEEEEIL KC+TFCVNY+LKPSDIVSSWEVYYLNRQLDGS+VQI
Sbjct: 1   MDDEIGEEFKRSGFTFDEEEEILNKCVTFCVNYSLKPSDIVSSWEVYYLNRQLDGSIVQI 60

Query: 61  SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH 120
           +QMDGFLQHLQNEQK+A+IK EPDLH+YSSKDVDMILNDEDED  EDILKTPNGKSQ LH
Sbjct: 61  AQMDGFLQHLQNEQKEAIIKEEPDLHVYSSKDVDMILNDEDEDPKEDILKTPNGKSQGLH 120

Query: 121 KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED 180
           K+RFD TPYTNEGLFSSGKPSKHVTPFGQR EKF VRFSIN VPD ET E EDNCENDED
Sbjct: 121 KDRFDLTPYTNEGLFSSGKPSKHVTPFGQRTEKFAVRFSINNVPDKETAEMEDNCENDED 180

Query: 181 AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQYEE 240
           AIIKKV PLQGCSLTV GSG GLE N RFM+D+VEDR NAL+NRI+K+AKAL ASG YEE
Sbjct: 181 AIIKKVLPLQGCSLTVHGSGFGLEPNCRFMYDRVEDRFNALENRIVKHAKALVASGLYEE 240

Query: 241 AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG 300
            VDPT ASQKSMFAVGMICCDG+GHLNDKST+LQ S EHSGGQ VRLELQNL+QFSIFPG
Sbjct: 241 VVDPTVASQKSMFAVGMICCDGDGHLNDKSTMLQSSVEHSGGQWVRLELQNLSQFSIFPG 300

Query: 301 QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM 360
           QVVGIEGHNPSGHCLIASKLVD FPLSAT+DPILPPAKK  L+HDIQP++Q ST+S+LSM
Sbjct: 301 QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKITLDHDIQPINQPSTESELSM 360

Query: 361 IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF 420
           IIASGPYTTTDN+LFEPLTELLAYA+RK PQLLILLGPFVDSEHSDIKRG VEMS EE+F
Sbjct: 361 IIASGPYTTTDNILFEPLTELLAYAKRKPPQLLILLGPFVDSEHSDIKRGTVEMSFEEIF 420

Query: 421 NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL 480
           NFE+LRRVQDY EHMGPDARVLLMPS+RDANH  VFPQPAFDIPS+L+LQIS LPNPGIL
Sbjct: 421 NFEVLRRVQDYVEHMGPDARVLLMPSIRDANHDFVFPQPAFDIPSDLRLQISSLPNPGIL 480

Query: 481 EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVLCWTQECWIS 540
           EANQ KIACCT DILRHLSGEELSRNLTKG+ NDRLSTLASHILRQR             
Sbjct: 481 EANQAKIACCTMDILRHLSGEELSRNLTKGTANDRLSTLASHILRQRS------------ 540

Query: 541 NVLIYFYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER 600
                FYPLYPPAEGVPLDFSLA +ALDISVIPDILILPSDMKHFVKVISLGEG ++EER
Sbjct: 541 -----FYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGRADEER 600

Query: 601 ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI 639
           A+CVCVNPGRLAKGEGGGTFVEL YFG PD+INAAVVSI
Sbjct: 601 ANCVCVNPGRLAKGEGGGTFVELKYFGTPDKINAAVVSI 622

BLAST of MC09g1848 vs. ExPASy TrEMBL
Match: A0A6J1DEX3 (DNA polymerase alpha subunit B OS=Momordica charantia OX=3673 GN=LOC111019803 PE=3 SV=1)

HSP 1 Score: 1222 bits (3162), Expect = 0.0
Identity = 621/639 (97.18%), Postives = 621/639 (97.18%), Query Frame = 0

Query: 1   MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI 60
           MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI
Sbjct: 1   MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI 60

Query: 61  SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH 120
           SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH
Sbjct: 61  SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH 120

Query: 121 KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED 180
           KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED
Sbjct: 121 KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED 180

Query: 181 AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQYEE 240
           AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQYEE
Sbjct: 181 AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQYEE 240

Query: 241 AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG 300
           AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG
Sbjct: 241 AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG 300

Query: 301 QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM 360
           QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM
Sbjct: 301 QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM 360

Query: 361 IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF 420
           IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF
Sbjct: 361 IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF 420

Query: 421 NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL 480
           NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL
Sbjct: 421 NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL 480

Query: 481 EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVLCWTQECWIS 540
           EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQR             
Sbjct: 481 EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRS------------ 540

Query: 541 NVLIYFYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER 600
                FYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER
Sbjct: 541 -----FYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER 600

Query: 601 ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI 639
           ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI
Sbjct: 601 ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI 622

BLAST of MC09g1848 vs. ExPASy TrEMBL
Match: A0A6J1DDL1 (DNA polymerase alpha subunit B OS=Momordica charantia OX=3673 GN=LOC111019803 PE=3 SV=1)

HSP 1 Score: 1209 bits (3127), Expect = 0.0
Identity = 617/639 (96.56%), Postives = 617/639 (96.56%), Query Frame = 0

Query: 1   MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI 60
           MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI
Sbjct: 1   MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI 60

Query: 61  SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH 120
           SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH
Sbjct: 61  SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH 120

Query: 121 KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED 180
           KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED
Sbjct: 121 KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED 180

Query: 181 AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQYEE 240
           AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDK    SNALDNRIMKYAKALSASGQYEE
Sbjct: 181 AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDK----SNALDNRIMKYAKALSASGQYEE 240

Query: 241 AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG 300
           AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG
Sbjct: 241 AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG 300

Query: 301 QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM 360
           QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM
Sbjct: 301 QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM 360

Query: 361 IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF 420
           IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF
Sbjct: 361 IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF 420

Query: 421 NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL 480
           NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL
Sbjct: 421 NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL 480

Query: 481 EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVLCWTQECWIS 540
           EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQR             
Sbjct: 481 EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRS------------ 540

Query: 541 NVLIYFYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER 600
                FYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER
Sbjct: 541 -----FYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER 600

Query: 601 ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI 639
           ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI
Sbjct: 601 ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI 618

BLAST of MC09g1848 vs. ExPASy TrEMBL
Match: A0A6J1FZE8 (DNA polymerase alpha subunit B OS=Cucurbita moschata OX=3662 GN=LOC111449321 PE=3 SV=1)

HSP 1 Score: 1074 bits (2778), Expect = 0.0
Identity = 540/639 (84.51%), Postives = 577/639 (90.30%), Query Frame = 0

Query: 1   MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI 60
           M++EIG EFKRSGFTFDEEEEIL KC+TFCVNY+LKPSDIVSSWEVYYLNRQLDGS+VQI
Sbjct: 1   MDDEIGEEFKRSGFTFDEEEEILNKCVTFCVNYSLKPSDIVSSWEVYYLNRQLDGSIVQI 60

Query: 61  SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH 120
           +QMDGFLQHLQNEQK+A+IK EPDLH+YSSKDVDMILNDEDED  EDILKTPNGKSQ LH
Sbjct: 61  AQMDGFLQHLQNEQKEAIIKEEPDLHVYSSKDVDMILNDEDEDPKEDILKTPNGKSQGLH 120

Query: 121 KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED 180
           K+RFD TPYTNEGLFSSGKPSKHVTPFGQR EKF VRFSIN VPD ET E EDNCENDED
Sbjct: 121 KDRFDLTPYTNEGLFSSGKPSKHVTPFGQRTEKFAVRFSINNVPDKETAEMEDNCENDED 180

Query: 181 AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQYEE 240
           AIIKKV PLQGCSLTV GSG GLE N RFM+D+VEDR NAL+NRI+K+AKAL ASG YEE
Sbjct: 181 AIIKKVLPLQGCSLTVHGSGFGLEPNCRFMYDRVEDRFNALENRIVKHAKALVASGLYEE 240

Query: 241 AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG 300
            VDPT ASQKSMFAVGMICCDG+GHLNDKST+LQ S EHSGGQ VRLELQNL+QFSIFPG
Sbjct: 241 VVDPTVASQKSMFAVGMICCDGDGHLNDKSTMLQSSVEHSGGQWVRLELQNLSQFSIFPG 300

Query: 301 QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM 360
           QVVGIEGHNPSGHCLIASKLVD FPLSAT+DPILPPAKK  L+HDIQP++Q ST+S+LSM
Sbjct: 301 QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKITLDHDIQPINQPSTESELSM 360

Query: 361 IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF 420
           IIASGPYTTTDN+LFEPLTELLAYA+RK PQLLILLGPFVDSEHSDIKRG VEMS EE+F
Sbjct: 361 IIASGPYTTTDNILFEPLTELLAYAKRKPPQLLILLGPFVDSEHSDIKRGTVEMSFEEIF 420

Query: 421 NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL 480
           NFE+LRRVQDY EHMGPDARVLLMPS+RDANH  VFPQPAFDIPS+L+LQIS LPNPGIL
Sbjct: 421 NFEVLRRVQDYVEHMGPDARVLLMPSIRDANHDFVFPQPAFDIPSDLRLQISSLPNPGIL 480

Query: 481 EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVLCWTQECWIS 540
           EANQ KIACCT DILRHLSGEELSRNLTKG+ NDRLSTLASHILRQR             
Sbjct: 481 EANQAKIACCTMDILRHLSGEELSRNLTKGTANDRLSTLASHILRQRS------------ 540

Query: 541 NVLIYFYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER 600
                FYPLYPPAEGVPLDFSLA +ALDISVIPDILILPSDMKHFVKVISLGEG ++EER
Sbjct: 541 -----FYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGRADEER 600

Query: 601 ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI 639
           A+CVCVNPGRLAKGEGGGTFVEL YFG PD+INAAVVSI
Sbjct: 601 ANCVCVNPGRLAKGEGGGTFVELKYFGTPDKINAAVVSI 622

BLAST of MC09g1848 vs. ExPASy TrEMBL
Match: A0A6J1HSI8 (DNA polymerase alpha subunit B OS=Cucurbita maxima OX=3661 GN=LOC111466380 PE=3 SV=1)

HSP 1 Score: 1069 bits (2765), Expect = 0.0
Identity = 538/639 (84.19%), Postives = 577/639 (90.30%), Query Frame = 0

Query: 1   MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI 60
           M++EIG EFKRSGFTFDEEEEILKKC+TFC+NY+LKPSDIVSSWEVYYLNRQLDGS+VQI
Sbjct: 1   MDDEIGEEFKRSGFTFDEEEEILKKCVTFCINYSLKPSDIVSSWEVYYLNRQLDGSIVQI 60

Query: 61  SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH 120
           +QMDGFLQHLQNEQK+A+IK EPDLH+YSSKDVDMILNDEDED  EDILKTPNGKSQ LH
Sbjct: 61  AQMDGFLQHLQNEQKEAIIKEEPDLHVYSSKDVDMILNDEDEDPKEDILKTPNGKSQGLH 120

Query: 121 KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED 180
           K+RFD TPYTNEGLFSSGKPSKHVTPFGQR EKF VRFSIN VPD ET E EDNCENDED
Sbjct: 121 KDRFDLTPYTNEGLFSSGKPSKHVTPFGQRTEKFAVRFSINNVPDKETAEVEDNCENDED 180

Query: 181 AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQYEE 240
           AIIKKV PLQGCSLTV GSG GLE N RFM+D+VEDR NAL+NRI+K+AKAL ASG YEE
Sbjct: 181 AIIKKVLPLQGCSLTVHGSGFGLEPNCRFMYDRVEDRFNALENRIVKHAKALVASGLYEE 240

Query: 241 AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG 300
            VDPT ASQKS+FAVGMICCDG+GHLNDKST+LQ S EHSGGQRVRLELQNL+QFSIFPG
Sbjct: 241 VVDPTVASQKSLFAVGMICCDGDGHLNDKSTMLQSSVEHSGGQRVRLELQNLSQFSIFPG 300

Query: 301 QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM 360
           QVVGIEGHNPSGHCLIASKLVD FPLSAT+DPILPPAKK AL+HDIQP+  SST S+LSM
Sbjct: 301 QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALDHDIQPI--SSTHSELSM 360

Query: 361 IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF 420
           IIASGPYTTTDN+LFEPLTELLAYA+RK PQLLILLGPFVDSEHSDIKRG VEMS EE+F
Sbjct: 361 IIASGPYTTTDNILFEPLTELLAYAKRKPPQLLILLGPFVDSEHSDIKRGTVEMSFEEIF 420

Query: 421 NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL 480
            FE+LRRVQDY EHMGPDARVLLMPS+RDANH  VFPQPAFDIPS+L+LQ+S LPNPGIL
Sbjct: 421 KFEVLRRVQDYVEHMGPDARVLLMPSIRDANHDFVFPQPAFDIPSDLRLQVSSLPNPGIL 480

Query: 481 EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVLCWTQECWIS 540
           EANQ KIACCT DILRHLSGEELSRNLTKG+ NDRLSTLASHILRQR             
Sbjct: 481 EANQAKIACCTMDILRHLSGEELSRNLTKGTANDRLSTLASHILRQRS------------ 540

Query: 541 NVLIYFYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER 600
                FYPLYPPAEGVPLDFSLA +ALDISVIPDILILPSDMKHFVKVISLGEG ++EER
Sbjct: 541 -----FYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGRADEER 600

Query: 601 ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI 639
           A+CVCVNPGRLAKGEGGGTFVEL YFG PD++NAAVVSI
Sbjct: 601 ANCVCVNPGRLAKGEGGGTFVELKYFGTPDKLNAAVVSI 620

BLAST of MC09g1848 vs. ExPASy TrEMBL
Match: A0A0A0LQA4 (DNA polymerase alpha subunit B OS=Cucumis sativus OX=3659 GN=Csa_1G039840 PE=3 SV=1)

HSP 1 Score: 1063 bits (2748), Expect = 0.0
Identity = 542/639 (84.82%), Postives = 571/639 (89.36%), Query Frame = 0

Query: 1   MEEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQI 60
           MEEEIG EFKRSGFTFDEEEEILKKC+TFCVNYNLKPSDIVSSWEVYYLNR LDGSVV++
Sbjct: 118 MEEEIGEEFKRSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRLLDGSVVEL 177

Query: 61  SQMDGFLQHLQNEQKQAVIKGEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH 120
            Q+DGFLQHLQNEQK+ VIKGEPDLH+YSSKDVD+ILNDE+ED NEDILKTPNGKSQSLH
Sbjct: 178 EQLDGFLQHLQNEQKETVIKGEPDLHVYSSKDVDVILNDENEDLNEDILKTPNGKSQSLH 237

Query: 121 KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED 180
           KER D TPYTNEGLFSSGKPSKH TPFGQR EKFVVRF+IN +PD  T  K DN ENDED
Sbjct: 238 KERLDLTPYTNEGLFSSGKPSKHETPFGQRTEKFVVRFNINNMPDMVTAVKVDNSENDED 297

Query: 181 AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQYEE 240
            IIKKV+PLQGCSLTV GSG GLE N RFM+DK EDR NALDNRIM++AKAL+ASG YEE
Sbjct: 298 TIIKKVRPLQGCSLTVHGSGFGLEPNCRFMYDKDEDRFNALDNRIMRHAKALAASGLYEE 357

Query: 241 AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG 300
           AVDPT ASQKS+FAVGMI CDGEGHLNDKS LLQ S  HSGGQRVRLELQNL+QFSIFPG
Sbjct: 358 AVDPTVASQKSIFAVGMIYCDGEGHLNDKSILLQSSIAHSGGQRVRLELQNLSQFSIFPG 417

Query: 301 QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDIQPMDQSSTQSKLSM 360
           QVVGIEGHNPSGHCLIASKLVD FPLSAT+DPILPPAKK ALNHDIQP++QSST S+LSM
Sbjct: 418 QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM 477

Query: 361 IIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEELF 420
           IIASGPYTTTDNLLFEPLTELLAYA+RK+PQLLILLGPFVDSEHSDIKRG VEMS EE+F
Sbjct: 478 IIASGPYTTTDNLLFEPLTELLAYAKRKVPQLLILLGPFVDSEHSDIKRGTVEMSFEEIF 537

Query: 421 NFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIPSELKLQISCLPNPGIL 480
            FE+LRRVQDYAEHMGPDARVLLMPS RDANH   FPQPAFDIPS+LKLQIS  PNP IL
Sbjct: 538 KFEVLRRVQDYAEHMGPDARVLLMPSTRDANHDFAFPQPAFDIPSDLKLQISSPPNPCIL 597

Query: 481 EANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVLCWTQECWIS 540
           EANQ KIACCT DILRHLSGEELSRNLTKG TNDRLSTLASHILRQR             
Sbjct: 598 EANQAKIACCTMDILRHLSGEELSRNLTKGGTNDRLSTLASHILRQRS------------ 657

Query: 541 NVLIYFYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEEER 600
                FYPLYPPAEGVPLDFSLA +ALDISVIPDILILPSDMKHFVKVISLGEGGSEEE+
Sbjct: 658 -----FYPLYPPAEGVPLDFSLAPQALDISVIPDILILPSDMKHFVKVISLGEGGSEEEQ 717

Query: 601 ASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI 639
           A CVCVNPGRLAKGEGGGTFVEL+YFG PDQINA VVSI
Sbjct: 718 ARCVCVNPGRLAKGEGGGTFVELNYFGRPDQINAMVVSI 739

BLAST of MC09g1848 vs. TAIR 10
Match: AT1G67630.1 (DNA polymerase alpha 2 )

HSP 1 Score: 721.1 bits (1860), Expect = 7.9e-208
Identity = 373/641 (58.19%), Postives = 480/641 (74.88%), Query Frame = 0

Query: 2   EEEIGAEFKRSGFTFDEEEEILKKCLTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIS 61
           +EEI  EFKRSGF   EE+EILK+C+T C+NY+LKPSD+VSSWE+Y+LNRQL    V+  
Sbjct: 4   DEEIKNEFKRSGFILVEEDEILKRCVTLCINYSLKPSDLVSSWELYHLNRQLLDQTVKKD 63

Query: 62  QMDGFLQHLQNEQKQAVIK-GEPDLHIYSSKDVDMILNDEDEDTNEDILKTPNGKSQSLH 121
            MDGF+ HLQNEQK+++++  E  LH+YS++DVDM+L+   EDT E+I+ TP  KSQ LH
Sbjct: 64  DMDGFVLHLQNEQKESIMRVEEAGLHLYSNRDVDMLLDGVQEDT-EEIVTTPTNKSQRLH 123

Query: 122 KERFDSTPYTNEGLFSSGKPSKHVTPFGQRREKFVVRFSINGVPDNETPEKEDNCENDED 181
            + FDS   + +  +S+GK   HVTPFG R EKFVV+F++  V  +      ++ EN ED
Sbjct: 124 PDPFDSISRSRDYGYSTGKSVGHVTPFGSRIEKFVVKFNVGNVAAHAENGNNNDVENSED 183

Query: 182 AIIKKVQPLQGCSLTVLGSGSGLEDNFRFMHDKVEDRSNALDNRIMKYAKALSASGQYEE 241
            IIK+VQ  Q CSL V  +GSG E   RFM+D+ EDR +AL+ RI+++A A +ASG YEE
Sbjct: 184 DIIKRVQTSQRCSLKV--NGSGPEPGCRFMYDRTEDRFSALEYRIVRHADAFAASGLYEE 243

Query: 242 AVDPTAASQKSMFAVGMICCDGEGHLNDKSTLLQCSFEHSGGQRVRLELQNLNQFSIFPG 301
            VDP  ASQ+S+FAVGMICCDGEGHLNDKS LLQ S E + GQRV ++L+ L+QFSIFPG
Sbjct: 244 QVDPAVASQRSIFAVGMICCDGEGHLNDKSILLQSSAERTSGQRVPVDLKRLDQFSIFPG 303

Query: 302 QVVGIEGHNPSGHCLIASKLVDYFPLSATVDPILPPAKKRALNHDI-QPMDQSSTQSKLS 361
           QVVGIEG NPSGH L ASKL+D  PL+ TVD  LPPAK++ L+ ++  P ++S  +S++S
Sbjct: 304 QVVGIEGQNPSGHYLTASKLLDSVPLTLTVDVDLPPAKRQILDQEVLVPAEESCQKSEVS 363

Query: 362 MIIASGPYTTTDNLLFEPLTELLAYARRKLPQLLILLGPFVDSEHSDIKRGIVEMSLEEL 421
            IIASGP+TT+DNLLFEPL ELLAYA+RK PQLL+LLGPFVDSEH +IK+G V+ +  E+
Sbjct: 364 FIIASGPFTTSDNLLFEPLNELLAYAKRKPPQLLVLLGPFVDSEHPEIKKGAVDATFNEI 423

Query: 422 FNFEILRRVQDYAEHMGPDARVLLMPSVRDANHAEVFPQPAFDIP-SELKLQISCLPNPG 481
           F  E+LR++QDY E MG + RV+L+PS+RDANH  +FPQP FDI   +L+ Q++ L NPG
Sbjct: 424 FQVEVLRKLQDYVEFMGSEVRVVLVPSIRDANHDFIFPQPPFDIHIPDLEHQMTSLSNPG 483

Query: 482 ILEANQVKIACCTTDILRHLSGEELSRNLTKGSTNDRLSTLASHILRQRRHVLCWTQECW 541
             EANQVK++CCT D+L+ LSGEE+S+N   G   DR+S LASH+LRQR           
Sbjct: 484 TFEANQVKVSCCTVDVLKQLSGEEMSKN-PSGVPTDRMSRLASHLLRQRS---------- 543

Query: 542 ISNVLIYFYPLYPPAEGVPLDFSLAAEALDISVIPDILILPSDMKHFVKVISLGEGGSEE 601
                  FYPLYPP E +P D SLA +AL IS IPDIL+LPSDM++FVK++SLGE   EE
Sbjct: 544 -------FYPLYPPPESLPYDSSLAPKALQISSIPDILLLPSDMRYFVKILSLGE---EE 603

Query: 602 ERASCVCVNPGRLAKGEGGGTFVELSYFGAPDQINAAVVSI 640
           + A CVCVNPGRLAKGEG GTFVEL+Y G P+ ++A+++SI
Sbjct: 604 DAAKCVCVNPGRLAKGEGAGTFVELTYKGGPESMHASIISI 620

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q141812.3e-6328.48DNA polymerase alpha subunit B OS=Homo sapiens OX=9606 GN=POLA2 PE=1 SV=2[more]
P336113.9e-5826.87DNA polymerase alpha subunit B OS=Mus musculus OX=10090 GN=Pola2 PE=1 SV=2[more]
O890433.9e-5827.06DNA polymerase alpha subunit B OS=Rattus norvegicus OX=10116 GN=Pola2 PE=1 SV=2[more]
Q58D133.4e-5426.47DNA polymerase alpha subunit B OS=Bos taurus OX=9913 GN=POLA2 PE=2 SV=1[more]
O749464.9e-4531.95DNA polymerase alpha subunit B OS=Schizosaccharomyces pombe (strain 972 / ATCC 2... [more]
Match NameE-valueIdentityDescription
XP_022151967.10.097.18DNA polymerase alpha subunit B isoform X1 [Momordica charantia][more]
XP_022151968.10.096.56DNA polymerase alpha subunit B isoform X2 [Momordica charantia][more]
KAG7012749.10.085.13DNA polymerase alpha subunit B [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023541613.10.084.66DNA polymerase alpha subunit B [Cucurbita pepo subsp. pepo][more]
XP_022944938.10.084.51DNA polymerase alpha subunit B [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1DEX30.097.18DNA polymerase alpha subunit B OS=Momordica charantia OX=3673 GN=LOC111019803 PE... [more]
A0A6J1DDL10.096.56DNA polymerase alpha subunit B OS=Momordica charantia OX=3673 GN=LOC111019803 PE... [more]
A0A6J1FZE80.084.51DNA polymerase alpha subunit B OS=Cucurbita moschata OX=3662 GN=LOC111449321 PE=... [more]
A0A6J1HSI80.084.19DNA polymerase alpha subunit B OS=Cucurbita maxima OX=3661 GN=LOC111466380 PE=3 ... [more]
A0A0A0LQA40.084.82DNA polymerase alpha subunit B OS=Cucumis sativus OX=3659 GN=Csa_1G039840 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT1G67630.17.9e-20858.19DNA polymerase alpha 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007185DNA polymerase alpha/delta/epsilon, subunit BPFAMPF04042DNA_pol_E_Bcoord: 361..587
e-value: 1.3E-31
score: 109.6
IPR013627DNA polymerase alpha, subunit B, N-terminalPFAMPF08418Pol_alpha_B_Ncoord: 21..240
e-value: 2.8E-8
score: 34.0
IPR043034DNA polymerase alpha, subunit B, N-terminal domain superfamilyGENE3D1.10.8.530coord: 1..77
e-value: 4.1E-7
score: 31.6
IPR016722DNA polymerase alpha, subunit BPIRSFPIRSF018300DNA_pol_alpha_2coord: 1..630
e-value: 2.7E-167
score: 555.8
IPR016722DNA polymerase alpha, subunit BPANTHERPTHR23061DNA POLYMERASE 2 ALPHA 70 KDA SUBUNITcoord: 1..639
NoneNo IPR availableGENE3D3.60.21.60coord: 545..635
e-value: 5.9E-18
score: 66.6
coord: 340..514
e-value: 1.1E-41
score: 144.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g1848.1MC09g1848.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006259 DNA metabolic process
biological_process GO:0006260 DNA replication
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding