MC09g1774 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g1774
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	DNA-directed RNA polymerase II subunit 10
Location	MC09: 22892774 .. 22893367 (+)
RNA-Seq Expression	MC09g1774
Synteny	MC09g1774

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAATTCCGGTGCGCTGTTTCACGTGCGGCAAGGTATTACTTCCGCTTCAATCGCTTTTTTCCTTATTTGATCGGCCATCGCCGTTCCTTCAATGGCTAAGGAAATTAATCTTCAGCTTTCTCTGCATTATTATAAGGAAACTCTGTTTGTATCCGTATTGTGTTTAATTTTATGTTCTGTTTTTTCTTTTTTTTTTTCAGGTGATCGGTAACAAATGGGACTCTTATCTAGAGCTTCTGCAAGCAGACTATCCGGAAGGGTAAAATAGAACGCCGAATTGGCTTCAATTCGTCTATTTCTAATTCCTCTTTCATAATCTTGTTAAATTCTGCTAACACAATCGTTCTGAATTTGCATTATGCAAGTATTTTGAGTTTGCGTAGGATCGTTTCATGTATTGAAATTTTTGCGATAACAGCCACGATTGGTAAAAAAAGTATATATTAATAGGATTGAAAAAGATGAAAACATAGTGATTTTTGGTGCGGTGCAGGGAGGCGTTGGATTTATTAGGGCTAGTTCGGTATTGCTGTCGGCGGATGCTTATGACTCATGTGGATCTGATCGAGAAGCTGTTGAACTACAACAGT

mRNA sequence

ATGATAATTCCGGTGCGCTGTTTCACGTGCGGCAAGGTGATCGGTAACAAATGGGACTCTTATCTAGAGCTTCTGCAAGCAGACTATCCGGAAGGGGAGGCGTTGGATTTATTAGGGCTAGTTCGGTATTGCTGTCGGCGGATGCTTATGACTCATGTGGATCTGATCGAGAAGCTGTTGAACTACAACAGT

Coding sequence (CDS)

ATGATAATTCCGGTGCGCTGTTTCACGTGCGGCAAGGTGATCGGTAACAAATGGGACTCTTATCTAGAGCTTCTGCAAGCAGACTATCCGGAAGGGGAGGCGTTGGATTTATTAGGGCTAGTTCGGTATTGCTGTCGGCGGATGCTTATGACTCATGTGGATCTGATCGAGAAGCTGTTGAACTACAACAGT

Protein sequence

MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLLNYNS

Homology

[Close]

BLAST of MC09g1774 vs. ExPASy Swiss-Prot
Match: Q9SYA6 (DNA-directed RNA polymerase subunit 10-like protein OS=Arabidopsis thaliana OX=3702 GN=NRPB10L PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 2.9e-30
Identity = 58/64 (90.62%), Postives = 62/64 (96.88%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MI+PVRCFTCGKVIGNKWD+YLELLQADY EG+ALD LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIVPVRCFTCGKVIGNKWDTYLELLQADYAEGDALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNS 65
          NYN+
Sbjct: 61 NYNT 64

[Close]

BLAST of MC09g1774 vs. ExPASy Swiss-Prot
Match: Q39290 (DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Brassica napus OX=3708 PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 9.3e-29
Identity = 56/64 (87.50%), Postives = 60/64 (93.75%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD+YL+LLQ DY EG+ALD L LVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDAYLDLLQLDYTEGDALDALNLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNS 65
          NYN+
Sbjct: 61 NYNT 64

[Close]

BLAST of MC09g1774 vs. ExPASy Swiss-Prot
Match: Q8LFJ6 (DNA-directed RNA polymerases II, IV and V subunit 10 OS=Arabidopsis thaliana OX=3702 GN=NRPB10 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 2.1e-28
Identity = 56/64 (87.50%), Postives = 59/64 (92.19%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD YL+LLQ DY EG+ALD L LVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDQYLDLLQLDYTEGDALDALQLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNS 65
          NYN+
Sbjct: 61 NYNT 64

[Close]

BLAST of MC09g1774 vs. ExPASy Swiss-Prot
Match: Q55AB6 (DNA-directed RNA polymerases I, II, and III subunit rpabc5 OS=Dictyostelium discoideum OX=44689 GN=polr2l PE=3 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 2.5e-26
Identity = 53/62 (85.48%), Postives = 56/62 (90.32%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD+YL LLQ DY EG+ALD L L RYCCRRML+THVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDAYLSLLQIDYTEGDALDALCLKRYCCRRMLLTHVDLIEKLL 60

Query: 61 NY 63
          NY
Sbjct: 61 NY 62

[Close]

BLAST of MC09g1774 vs. ExPASy Swiss-Prot
Match: Q9VC49 (DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Drosophila melanogaster OX=7227 GN=Rpb10 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 3.3e-26
Identity = 52/62 (83.87%), Postives = 57/62 (91.94%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIP+RCFTCGKVIGNKW+SYL LLQA+Y EG+ALD LGL RYCCRRML+ HVDLIEKLL
Sbjct: 1  MIIPIRCFTCGKVIGNKWESYLGLLQAEYTEGDALDALGLKRYCCRRMLLGHVDLIEKLL 60

Query: 61 NY 63
          NY
Sbjct: 61 NY 62

[Close]

BLAST of MC09g1774 vs. NCBI nr
Match: XP_022151052.1 (DNA-directed RNA polymerase subunit 10-like protein [Momordica charantia])

HSP 1 Score: 140 bits (352), Expect = 8.53e-42
Identity = 63/64 (98.44%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNS 64
          NYN+
Sbjct: 61 NYNT 64

[Close]

BLAST of MC09g1774 vs. NCBI nr
Match: XP_038893151.1 (DNA-directed RNA polymerase subunit 10-like protein [Benincasa hispida])

HSP 1 Score: 138 bits (347), Expect = 4.95e-41
Identity = 61/64 (95.31%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD+YLELLQADYPEGEALD+LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLELLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNS 64
          NYN+
Sbjct: 61 NYNT 64

[Close]

BLAST of MC09g1774 vs. NCBI nr
Match: XP_004135479.1 (DNA-directed RNA polymerase subunit 10-like protein [Cucumis sativus] >KGN51793.1 hypothetical protein Csa_009052 [Cucumis sativus])

HSP 1 Score: 137 bits (344), Expect = 1.42e-40
Identity = 60/64 (93.75%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD+YL+LLQADYPEGEALD+LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNS 64
          NYN+
Sbjct: 61 NYNT 64

[Close]

BLAST of MC09g1774 vs. NCBI nr
Match: XP_008446194.1 (PREDICTED: DNA-directed RNA polymerase subunit 10-like protein [Cucumis melo] >KAA0034305.1 DNA-directed RNA polymerase subunit 10-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 135 bits (341), Expect = 4.08e-40
Identity = 60/64 (93.75%), Postives = 63/64 (98.44%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD+YL+LLQADYPEGEALD LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYPEGEALDTLGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNS 64
          NYN+
Sbjct: 61 NYNT 64

[Close]

BLAST of MC09g1774 vs. NCBI nr
Match: EFJ10727.1 (hypothetical protein SELMODRAFT_127452 [Selaginella moellendorffii] >EFJ35775.1 hypothetical protein SELMODRAFT_79899 [Selaginella moellendorffii])

HSP 1 Score: 134 bits (338), Expect = 9.91e-40
Identity = 60/64 (93.75%), Postives = 62/64 (96.88%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD+YL+LLQADYPE EALD LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYPESEALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNS 64
          NYNS
Sbjct: 61 NYNS 64

[Close]

BLAST of MC09g1774 vs. ExPASy TrEMBL
Match: A0A6J1DCG7 (DNA-directed RNA polymerase subunit 10-like protein OS=Momordica charantia OX=3673 GN=LOC111019076 PE=3 SV=1)

HSP 1 Score: 140 bits (352), Expect = 4.13e-42
Identity = 63/64 (98.44%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNS 64
          NYN+
Sbjct: 61 NYNT 64

[Close]

BLAST of MC09g1774 vs. ExPASy TrEMBL
Match: A0A0A0KQ80 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G600940 PE=3 SV=1)

HSP 1 Score: 137 bits (344), Expect = 6.87e-41
Identity = 60/64 (93.75%), Postives = 64/64 (100.00%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD+YL+LLQADYPEGEALD+LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYPEGEALDMLGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNS 64
          NYN+
Sbjct: 61 NYNT 64

[Close]

BLAST of MC09g1774 vs. ExPASy TrEMBL
Match: A0A5A7SYN0 (DNA-directed RNA polymerase subunit 10-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003050 PE=3 SV=1)

HSP 1 Score: 135 bits (341), Expect = 1.97e-40
Identity = 60/64 (93.75%), Postives = 63/64 (98.44%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD+YL+LLQADYPEGEALD LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYPEGEALDTLGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNS 64
          NYN+
Sbjct: 61 NYNT 64

[Close]

BLAST of MC09g1774 vs. ExPASy TrEMBL
Match: A0A1S3BEG8 (DNA-directed RNA polymerase subunit 10-like protein OS=Cucumis melo OX=3656 GN=LOC103488997 PE=3 SV=1)

HSP 1 Score: 135 bits (341), Expect = 1.97e-40
Identity = 60/64 (93.75%), Postives = 63/64 (98.44%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD+YL+LLQADYPEGEALD LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYPEGEALDTLGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNS 64
          NYN+
Sbjct: 61 NYNT 64

[Close]

BLAST of MC09g1774 vs. ExPASy TrEMBL
Match: D8QXA0 (Uncharacterized protein OS=Selaginella moellendorffii OX=88036 GN=SELMODRAFT_127452 PE=3 SV=1)

HSP 1 Score: 134 bits (338), Expect = 4.80e-40
Identity = 60/64 (93.75%), Postives = 62/64 (96.88%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD+YL+LLQADYPE EALD LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDTYLDLLQADYPESEALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNS 64
          NYNS
Sbjct: 61 NYNS 64

[Close]

BLAST of MC09g1774 vs. TAIR 10
Match: AT1G61700.1 (RNA polymerases N / 8 kDa subunit )

HSP 1 Score: 131.7 bits (330), Expect = 2.1e-31
Identity = 58/64 (90.62%), Postives = 62/64 (96.88%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MI+PVRCFTCGKVIGNKWD+YLELLQADY EG+ALD LGLVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIVPVRCFTCGKVIGNKWDTYLELLQADYAEGDALDALGLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNS 65
          NYN+
Sbjct: 61 NYNT 64

[Close]

BLAST of MC09g1774 vs. TAIR 10
Match: AT1G11475.1 (RNA polymerases N / 8 kDa subunit )

HSP 1 Score: 125.6 bits (314), Expect = 1.5e-29
Identity = 56/64 (87.50%), Postives = 59/64 (92.19%), Query Frame = 0


Query: 1  MIIPVRCFTCGKVIGNKWDSYLELLQADYPEGEALDLLGLVRYCCRRMLMTHVDLIEKLL 60
          MIIPVRCFTCGKVIGNKWD YL+LLQ DY EG+ALD L LVRYCCRRMLMTHVDLIEKLL
Sbjct: 1  MIIPVRCFTCGKVIGNKWDQYLDLLQLDYTEGDALDALQLVRYCCRRMLMTHVDLIEKLL 60

Query: 61 NYNS 65
          NYN+
Sbjct: 61 NYNT 64

The following BLAST results are available for this feature:

BLAST of MC09g1774 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SYA6	2.9e-30	90.63	DNA-directed RNA polymerase subunit 10-like protein OS=Arabidopsis thaliana OX=3...	[more]
Q39290	9.3e-29	87.50	DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Brassica napus OX=...	[more]
Q8LFJ6	2.1e-28	87.50	DNA-directed RNA polymerases II, IV and V subunit 10 OS=Arabidopsis thaliana OX=...	[more]
Q55AB6	2.5e-26	85.48	DNA-directed RNA polymerases I, II, and III subunit rpabc5 OS=Dictyostelium disc...	[more]
Q9VC49	3.3e-26	83.87	DNA-directed RNA polymerases I, II, and III subunit RPABC5 OS=Drosophila melanog...	[more]

BLAST of MC09g1774 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151052.1	8.53e-42	98.44	DNA-directed RNA polymerase subunit 10-like protein [Momordica charantia]	[more]
XP_038893151.1	4.95e-41	95.31	DNA-directed RNA polymerase subunit 10-like protein [Benincasa hispida]	[more]
XP_004135479.1	1.42e-40	93.75	DNA-directed RNA polymerase subunit 10-like protein [Cucumis sativus] >KGN51793....	[more]
XP_008446194.1	4.08e-40	93.75	PREDICTED: DNA-directed RNA polymerase subunit 10-like protein [Cucumis melo] >K...	[more]
EFJ10727.1	9.91e-40	93.75	hypothetical protein SELMODRAFT_127452 [Selaginella moellendorffii] >EFJ35775.1 ...	[more]

BLAST of MC09g1774 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DCG7	4.13e-42	98.44	DNA-directed RNA polymerase subunit 10-like protein OS=Momordica charantia OX=36...	[more]
A0A0A0KQ80	6.87e-41	93.75	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G600940 PE=3 SV=1	[more]
A0A5A7SYN0	1.97e-40	93.75	DNA-directed RNA polymerase subunit 10-like protein OS=Cucumis melo var. makuwa ...	[more]
A0A1S3BEG8	1.97e-40	93.75	DNA-directed RNA polymerase subunit 10-like protein OS=Cucumis melo OX=3656 GN=L...	[more]
D8QXA0	4.80e-40	93.75	Uncharacterized protein OS=Selaginella moellendorffii OX=88036 GN=SELMODRAFT_127...	[more]

BLAST of MC09g1774 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G61700.1	2.1e-31	90.63	RNA polymerases N / 8 kDa subunit	[more]
AT1G11475.1	1.5e-29	87.50	RNA polymerases N / 8 kDa subunit	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000268	DNA-directed RNA polymerase, subunit N/Rpb10	PFAM	PF01194	RNA_pol_N	coord: 1..59 e-value: 3.4E-31 score: 107.3
IPR000268	DNA-directed RNA polymerase, subunit N/Rpb10	PIRSF	PIRSF005653	RpoN_RPB10	coord: 1..64 e-value: 7.5E-31 score: 103.9
IPR000268	DNA-directed RNA polymerase, subunit N/Rpb10	PANTHER	PTHR23431	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER	coord: 1..63
IPR000268	DNA-directed RNA polymerase, subunit N/Rpb10	HAMAP	MF_00250	RNApol_arch_N	coord: 1..63 score: 19.677124
None	No IPR available	GENE3D	1.10.10.60		coord: 1..64 e-value: 2.6E-38 score: 132.0
None	No IPR available	PANTHER	PTHR23431:SF6	SUBFAMILY NOT NAMED	coord: 1..63
IPR020789	RNA polymerases, subunit N, zinc binding site	PROSITE	PS01112	RNA_POL_N_8KD	coord: 2..11
IPR023580	RNA polymerase subunit RPB10	SUPERFAMILY	46924	RNA polymerase subunit RPB10	coord: 1..63

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g1774.1	MC09g1774.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006360	transcription by RNA polymerase I
biological_process	GO:0006366	transcription by RNA polymerase II
biological_process	GO:0042797	tRNA transcription by RNA polymerase III
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005736	RNA polymerase I complex
cellular_component	GO:0005665	RNA polymerase II, core complex
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0008270	zinc ion binding