MC09g1748 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g1748
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionRING-H2 finger protein ATL47
LocationMC09: 22698946 .. 22700004 (+)
RNA-Seq ExpressionMC09g1748
SyntenyMC09g1748
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTTTCTTCATCCTCAAACCAGAGTCTTTCTGGTTACTCCCTCCATTTCCCCTCTGCATTCCTCTTCCCCATTGGCATACCAAAAACAACCAACTCCACCCACTTCCTCTAGCAGTAAAATAAGCCCTGCAGTTCTTTTAGTCATAGTTATTCTGGCTATTCTTTTTTTCATATCTGGTATTCTTCATTTTCTAGTGAGATTTCTTGTAAAGCAACGATCTCCATCATCAATATCTGAGTCCAATAGTAATAGGTTCCCTGAAATGTCTGAATCATCTGCTTTCCAAAGACAGTTACAACAGCTCTTCCATCTTCATGATTCAGGGCTAGATCAAGCCTTCATAGACGCCCTCCCTGTTTTCCTCTACACAGAGATAGTGGGTTTAAAAGAGCCATTTGATTGTGCTGTATGTCTTTGTGAATTTTCAGAACTAGACAAGCTAAGATTGCTCCCTACTTGCAGTCATGCTTTTCACATTGATTGTATAGACACATGGTTGCTTTCTAACTCAACTTGCCCTCTTTGTAGAGGGACCCTTTACTCTCCTGGGCTTGCCATTGAAAATCCCATATTTGGTTTTGAAGACTCAGAGGGAACAGAGGGGTCTGCAGAGGATGGAAGAATTGGTGTTCCTACAGCTCAAAAACCTCCAGAGAACGACATGTATGGTGAAAAGAGAGTGTTTTCAATCAGACTTGGGAAATTTAGGAACTTGAATAATGGAGGAGTGAGAGGATTGGAGAAAGGAGAGGGAGAGACCAGCAGTAGCAGTTTGGGGGCGAGAAGATGCTATTCAATGGGGTCCTACCAGTATATCGTGGCTGATTCAGATTTGCAAGTGGCCTTACGAACGAGTAATCGGACTGGTGGGCGAAGTATGAGATTCGTGAGAGCTAGAGATTCTCAAACTGGGAATTCCTTGATTGATGATGATGCTGAGGGGAAGAAGATTAACATTGGAAGTAAAAATGAAAGCTTTTCTGTTTCCAAAATCTGGCTGTGGTCAAAGAAGGCTCGACAGCCGATTGGTTCAGACGTTCATCTGCGAGCTAAT

mRNA sequence

ATGTCTTTTCTTCATCCTCAAACCAGAGTCTTTCTGGTTACTCCCTCCATTTCCCCTCTGCATTCCTCTTCCCCATTGGCATACCAAAAACAACCAACTCCACCCACTTCCTCTAGCAGTAAAATAAGCCCTGCAGTTCTTTTAGTCATAGTTATTCTGGCTATTCTTTTTTTCATATCTGGTATTCTTCATTTTCTAGTGAGATTTCTTGTAAAGCAACGATCTCCATCATCAATATCTGAGTCCAATAGTAATAGGTTCCCTGAAATGTCTGAATCATCTGCTTTCCAAAGACAGTTACAACAGCTCTTCCATCTTCATGATTCAGGGCTAGATCAAGCCTTCATAGACGCCCTCCCTGTTTTCCTCTACACAGAGATAGTGGGTTTAAAAGAGCCATTTGATTGTGCTGTATGTCTTTGTGAATTTTCAGAACTAGACAAGCTAAGATTGCTCCCTACTTGCAGTCATGCTTTTCACATTGATTGTATAGACACATGGTTGCTTTCTAACTCAACTTGCCCTCTTTGTAGAGGGACCCTTTACTCTCCTGGGCTTGCCATTGAAAATCCCATATTTGGTTTTGAAGACTCAGAGGGAACAGAGGGGTCTGCAGAGGATGGAAGAATTGGTGTTCCTACAGCTCAAAAACCTCCAGAGAACGACATGTATGGTGAAAAGAGAGTGTTTTCAATCAGACTTGGGAAATTTAGGAACTTGAATAATGGAGGAGTGAGAGGATTGGAGAAAGGAGAGGGAGAGACCAGCAGTAGCAGTTTGGGGGCGAGAAGATGCTATTCAATGGGGTCCTACCAGTATATCGTGGCTGATTCAGATTTGCAAGTGGCCTTACGAACGAGTAATCGGACTGGTGGGCGAAGTATGAGATTCGTGAGAGCTAGAGATTCTCAAACTGGGAATTCCTTGATTGATGATGATGCTGAGGGGAAGAAGATTAACATTGGAAGTAAAAATGAAAGCTTTTCTGTTTCCAAAATCTGGCTGTGGTCAAAGAAGGCTCGACAGCCGATTGGTTCAGACGTTCATCTGCGAGCTAAT

Coding sequence (CDS)

ATGTCTTTTCTTCATCCTCAAACCAGAGTCTTTCTGGTTACTCCCTCCATTTCCCCTCTGCATTCCTCTTCCCCATTGGCATACCAAAAACAACCAACTCCACCCACTTCCTCTAGCAGTAAAATAAGCCCTGCAGTTCTTTTAGTCATAGTTATTCTGGCTATTCTTTTTTTCATATCTGGTATTCTTCATTTTCTAGTGAGATTTCTTGTAAAGCAACGATCTCCATCATCAATATCTGAGTCCAATAGTAATAGGTTCCCTGAAATGTCTGAATCATCTGCTTTCCAAAGACAGTTACAACAGCTCTTCCATCTTCATGATTCAGGGCTAGATCAAGCCTTCATAGACGCCCTCCCTGTTTTCCTCTACACAGAGATAGTGGGTTTAAAAGAGCCATTTGATTGTGCTGTATGTCTTTGTGAATTTTCAGAACTAGACAAGCTAAGATTGCTCCCTACTTGCAGTCATGCTTTTCACATTGATTGTATAGACACATGGTTGCTTTCTAACTCAACTTGCCCTCTTTGTAGAGGGACCCTTTACTCTCCTGGGCTTGCCATTGAAAATCCCATATTTGGTTTTGAAGACTCAGAGGGAACAGAGGGGTCTGCAGAGGATGGAAGAATTGGTGTTCCTACAGCTCAAAAACCTCCAGAGAACGACATGTATGGTGAAAAGAGAGTGTTTTCAATCAGACTTGGGAAATTTAGGAACTTGAATAATGGAGGAGTGAGAGGATTGGAGAAAGGAGAGGGAGAGACCAGCAGTAGCAGTTTGGGGGCGAGAAGATGCTATTCAATGGGGTCCTACCAGTATATCGTGGCTGATTCAGATTTGCAAGTGGCCTTACGAACGAGTAATCGGACTGGTGGGCGAAGTATGAGATTCGTGAGAGCTAGAGATTCTCAAACTGGGAATTCCTTGATTGATGATGATGCTGAGGGGAAGAAGATTAACATTGGAAGTAAAAATGAAAGCTTTTCTGTTTCCAAAATCTGGCTGTGGTCAAAGAAGGCTCGACAGCCGATTGGTTCAGACGTTCATCTGCGAGCTAAT

Protein sequence

MSFLHPQTRVFLVTPSISPLHSSSPLAYQKQPTPPTSSSSKISPAVLLVIVILAILFFISGILHFLVRFLVKQRSPSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTLYSPGLAIENPIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKRVFSIRLGKFRNLNNGGVRGLEKGEGETSSSSLGARRCYSMGSYQYIVADSDLQVALRTSNRTGGRSMRFVRARDSQTGNSLIDDDAEGKKINIGSKNESFSVSKIWLWSKKARQPIGSDVHLRAN
Homology
BLAST of MC09g1748 vs. ExPASy Swiss-Prot
Match: Q8GW38 (RING-H2 finger protein ATL47 OS=Arabidopsis thaliana OX=3702 GN=ATL47 PE=2 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 4.3e-91
Identity = 199/332 (59.94%), Postives = 230/332 (69.28%), Query Frame = 0

Query: 17  ISPLHSSSPLAYQKQPTPPTSSSS-----KISPAVLLVIVILAILFFISGILHFLVR-FL 76
           ISP  + SP+    Q T  +SSSS     +ISP +L +IV+L+++FFI  ILH LVR +L
Sbjct: 19  ISPSSAPSPITLNHQLTDSSSSSSSGGNNRISPIILFIIVLLSVIFFICSILHLLVRYYL 78

Query: 77  VKQRSPSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYTEIVGL 136
            K+RS  S S + SN+ PE S+S  +QRQLQQLFHLHDSGLDQA IDALPVFLY EI G 
Sbjct: 79  KKKRSNLSSSPNESNQNPEFSDSDTYQRQLQQLFHLHDSGLDQALIDALPVFLYKEIKGT 138

Query: 137 KEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTLYSPGLAIEN 196
           KEPFDCAVCLCEFSE DKLRLLP CSHAFHIDCIDTWLLSNSTCPLCRGTL+S G   E 
Sbjct: 139 KEPFDCAVCLCEFSEDDKLRLLPNCSHAFHIDCIDTWLLSNSTCPLCRGTLFSLGHQFEY 198

Query: 197 PIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKRVFSIRLGKFRN---LNNGGVRG 256
           P F F    G +G    G + V   QKP EN++   KRVFS+RLGKFR+   +NNG V  
Sbjct: 199 PDFNFGFFAGDDGG---GGVRVSPVQKPAENEI--GKRVFSVRLGKFRSSNIVNNGEV-- 258

Query: 257 LEKGEGETSSSSLGARRCYSMGSYQYIVADSDLQVALRTSNRTGGRSMRFVRARDSQTGN 316
           +  G GETSSSSL  RRC+SMGSYQYIVA+SDL VAL   N  G ++             
Sbjct: 259 VVGGGGETSSSSLDNRRCFSMGSYQYIVAESDLVVAL-CPNNEGLKN------------- 318

Query: 317 SLIDDDAEGKKINIGSKNESFSVSKIWLWSKK 340
              + D EGKKIN+ SK ESFSVSKIW WS K
Sbjct: 319 ---NKDVEGKKINMRSKGESFSVSKIWQWSNK 326

BLAST of MC09g1748 vs. ExPASy Swiss-Prot
Match: Q9FL07 (RING-H2 finger protein ATL46 OS=Arabidopsis thaliana OX=3702 GN=ATL46 PE=2 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 8.1e-90
Identity = 192/320 (60.00%), Postives = 229/320 (71.56%), Query Frame = 0

Query: 34  PPTSSSSKISPAVLLVIVILAILFFISGILHFLVRFLVKQRSPSSISESNSNRFPEMSES 93
           PP SS ++ISPAVL VIVILA+LFFISG+LH LVRFL+K   PS+ + S SNRFPE+S S
Sbjct: 33  PPPSSGNRISPAVLFVIVILAVLFFISGLLHLLVRFLIKH--PSATASSRSNRFPEISTS 92

Query: 94  SAFQRQLQQLFHLHDSGLDQAFIDALPVFLYTEIVG----------LKEPFDCAVCLCEF 153
            A QRQLQQLFHL+DSGLDQAFIDALPVF Y EIVG           +EPFDCAVCLCEF
Sbjct: 93  DALQRQLQQLFHLNDSGLDQAFIDALPVFHYKEIVGSAGGGGGNGAAQEPFDCAVCLCEF 152

Query: 154 SELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTLYSPGLAIENPIFGFED-SEGTE 213
           SE DKLRLLP CSHAFH++CIDTWL SNSTCPLCRGTL+SPG ++ENP+F F+D  E  E
Sbjct: 153 SEKDKLRLLPMCSHAFHLNCIDTWLQSNSTCPLCRGTLFSPGFSMENPMFDFDDIREDEE 212

Query: 214 GSAEDGRIGVPTAQKPPE-NDMYGEKRVFSIRLGKFRNLNN-GGVRGLE-KGEGETSSSS 273
           G  E+G      +QK  E  ++  EK V  +RLGKF+ L+N G  +G +    GETSSS+
Sbjct: 213 GVTENG------SQKTMEIQEIVVEKGVLPVRLGKFKRLDNVGNGQGQDVVAGGETSSSN 272

Query: 274 LGARRCYSMGSYQYIVADSDLQVALRTSNRTGGRSMRFVRARDSQTGNSLIDDDAEGKKI 333
           L ARRC+SMGSYQYI+ +S+L+V            ++       QTGNS  +D+   KKI
Sbjct: 273 LDARRCFSMGSYQYILGNSELKVPFANDRLP---RLKPQDKESEQTGNSSSEDN---KKI 332

Query: 334 NIGSKNESFSVSKIWLWSKK 340
           N  +K ESFSVSKIWLW KK
Sbjct: 333 NTVAKGESFSVSKIWLWPKK 338

BLAST of MC09g1748 vs. ExPASy Swiss-Prot
Match: Q940Q4 (RING-H2 finger protein ATL13 OS=Arabidopsis thaliana OX=3702 GN=ATL13 PE=2 SV=2)

HSP 1 Score: 223.4 bits (568), Expect = 4.0e-57
Identity = 162/396 (40.91%), Postives = 215/396 (54.29%), Query Frame = 0

Query: 1   MSFLHPQTRVFLVTPSISPLHSSSPLAYQKQPTPPTSSSSKISPAVLLVIVILAILFFIS 60
           M ++ P+ +      S S L    PL+ +         +SKISP++LL+I+IL+I+FFIS
Sbjct: 8   MKWVFPEIKTTQNFLSPSSLPQEPPLSLRSSAN--FDLNSKISPSILLIIIILSIIFFIS 67

Query: 61  GILHFLVRFLVKQRSPSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALP 120
           G+LH LVRFL        ++ S+ +R       +A Q QLQQLFHLHDSG+DQ+FID LP
Sbjct: 68  GLLHLLVRFL--------LTPSSRDREDYFDNVTALQGQLQQLFHLHDSGVDQSFIDTLP 127

Query: 121 VFLYTEIVGLKE-PFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRG 180
           VF Y  I+GLK  PFDCAVCLCEF   DKLRLLP CSHAFH+DCIDTWLLS+STCPLCR 
Sbjct: 128 VFHYKSIIGLKNYPFDCAVCLCEFETEDKLRLLPKCSHAFHMDCIDTWLLSHSTCPLCRS 187

Query: 181 TLYSPGLAIENP------------------IFGFEDSEGTEGSAED--------GRIG-- 240
           +L S   + ++P                  I G  DS     + +D        G +G  
Sbjct: 188 SLLSDLSSHQDPRSSFLLVLESASDHSSREIGGDRDSAACVAANDDIDVSSAHLGLVGNN 247

Query: 241 --VPTAQKPPENDMY---------GEKRVFSIRLGKFRNLNNGGVRGLEKGEGETSSSSL 300
               T       D Y         G+   FS++LGKFRN++ G   G        +SSSL
Sbjct: 248 DLGSTRIDSGHGDQYLDGELGGSVGKVVPFSVKLGKFRNIDIG--EGTSSNNNIGNSSSL 307

Query: 301 GARRCYSMGSYQYIV-ADSDLQVALRT-----------SNRTGGRSMRF---VRARDSQT 342
             RRC+SMGSY+YI+  ++ L+V + T            +RT      F    R + S +
Sbjct: 308 DERRCFSMGSYEYIMDEETTLKVHVSTKKQSSKNRGLPGHRTAMSECGFDPTGRLKFSGS 367

BLAST of MC09g1748 vs. ExPASy Swiss-Prot
Match: Q9ZV53 (Putative RING-H2 finger protein ATL49 OS=Arabidopsis thaliana OX=3702 GN=ATL49 PE=3 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 4.9e-55
Identity = 147/345 (42.61%), Postives = 197/345 (57.10%), Query Frame = 0

Query: 25  PLAYQKQPTPPTSSSSKISPAVLLVIVILAILFFISGILHFLVRFLVKQRSPSSISESNS 84
           PL   K  T  ++ +SKI+P +LL+I+IL+I+FFISG+LH LV+FL        ++ S  
Sbjct: 22  PLIPLKSNTSLSNLNSKITPNILLIIIILSIIFFISGLLHILVKFL--------LTPSRE 81

Query: 85  NRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYTEIVGLK-EPFDCAVCLCEF 144
           +R       +A Q QLQQLF+LHDSG+DQ+ ID LPVF Y  IVGLK  PFDC VCLCEF
Sbjct: 82  SREDYFDNVTALQGQLQQLFNLHDSGVDQSLIDTLPVFHYKSIVGLKISPFDCPVCLCEF 141

Query: 145 SELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTLYSPGLAIEN------------- 204
              DKLRLLP CSHAFH++CIDTWLLS+STCPLCR  L S   +  N             
Sbjct: 142 ETEDKLRLLPKCSHAFHVECIDTWLLSHSTCPLCRSNLLSGFSSHHNLSSSYLLVLESEQ 201

Query: 205 ------PIFGFEDSEG--TEGSAEDGRIGVPTAQKPPENDMYG--EKRV-FSIRLGKFRN 264
                 P+       G      +E  RI        P+ DM G  EK V   ++LGKFRN
Sbjct: 202 SSRDMVPVLESNSQLGYDVNNDSESTRIRSGRKSCDPDGDMDGLDEKVVPLEVKLGKFRN 261

Query: 265 LNNGGVRGLEKGEG-ETSSSSLGARRCYSMGSYQYIV-ADSDLQVALRTSNRTGGRSMRF 324
           +++ G    +K      +S ++  RRC SMGSY+YI+  ++ L+V + T   +G   +  
Sbjct: 262 IDHVGEGSDQKKNSISGNSKNVDGRRCLSMGSYEYIMDQEATLKVHVSTKKLSGKDRVPS 321

Query: 325 VRARDSQTGNSLIDDDAEGKKINIGSKNESFSVSKIWLWSKKARQ 343
            R   S+ G    D   +G + ++  + ESFS+SKIWL  KK +Q
Sbjct: 322 HRTVMSECG---FDPTVKGIEKSV-VERESFSLSKIWLRGKKEKQ 354

BLAST of MC09g1748 vs. ExPASy Swiss-Prot
Match: Q7X843 (RING-H2 finger protein ATL48 OS=Arabidopsis thaliana OX=3702 GN=ATL48 PE=1 SV=2)

HSP 1 Score: 187.2 bits (474), Expect = 3.2e-46
Identity = 126/291 (43.30%), Postives = 164/291 (56.36%), Query Frame = 0

Query: 14  TPSISPLHSSS---PLAYQKQPTPPTSSSSKISPAVLLVIVILAILFFISGILHFLVRFL 73
           T ++SP  SS+    L  QK P     SSS I   + LVI  LA++    G+L+ + +FL
Sbjct: 90  TEALSPKSSSAATLTLMNQKDP-----SSSSIVSVLCLVISGLALIIVFLGVLYLIFKFL 149

Query: 74  VKQRSPSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYTEI-VG 133
            K  +   I   N N  P++   S+   QLQ LF LHDSGLDQ  IDALPVFLY  + + 
Sbjct: 150 RKSSTLFPIPHFNYN--PDLFSFSS--PQLQHLFFLHDSGLDQTAIDALPVFLYGNVTIS 209

Query: 134 LKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTL------YS 193
           L++PFDCAVCL EFS+ DKLRLLP CSHAFH+ CIDTWLLSNSTCPLCR +L      Y+
Sbjct: 210 LEQPFDCAVCLNEFSDTDKLRLLPVCSHAFHLHCIDTWLLSNSTCPLCRRSLSTSNVCYN 269

Query: 194 PGLAIENPIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKRVFSIRLGKFRNLNNG 253
               +  P+ G +         +DG+  +              KRVFS+RLG+F++ N  
Sbjct: 270 HSETLVAPLSGHQ-------QVDDGKASL-------------AKRVFSVRLGRFKSTNE- 329

Query: 254 GVRGLEKGEGETSSSSLGA---RRCYSMGSYQYIVADSDLQVALRTSNRTG 292
                 + +       +G    RRCYSMG+ QY+V D D  VAL +S R G
Sbjct: 330 -----SQSQRHDVKDEIGVRMPRRCYSMGTQQYLVCDQDFVVALSSSPREG 345

BLAST of MC09g1748 vs. NCBI nr
Match: XP_022151161.1 (RING-H2 finger protein ATL47 [Momordica charantia] >XP_022151162.1 RING-H2 finger protein ATL47 [Momordica charantia])

HSP 1 Score: 694 bits (1792), Expect = 6.02e-252
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0

Query: 1   MSFLHPQTRVFLVTPSISPLHSSSPLAYQKQPTPPTSSSSKISPAVLLVIVILAILFFIS 60
           MSFLHPQTRVFLVTPSISPLHSSSPLAYQKQPTPPTSSSSKISPAVLLVIVILAILFFIS
Sbjct: 1   MSFLHPQTRVFLVTPSISPLHSSSPLAYQKQPTPPTSSSSKISPAVLLVIVILAILFFIS 60

Query: 61  GILHFLVRFLVKQRSPSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALP 120
           GILHFLVRFLVKQRSPSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALP
Sbjct: 61  GILHFLVRFLVKQRSPSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALP 120

Query: 121 VFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGT 180
           VFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGT
Sbjct: 121 VFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGT 180

Query: 181 LYSPGLAIENPIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKRVFSIRLGKFRNL 240
           LYSPGLAIENPIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKRVFSIRLGKFRNL
Sbjct: 181 LYSPGLAIENPIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKRVFSIRLGKFRNL 240

Query: 241 NNGGVRGLEKGEGETSSSSLGARRCYSMGSYQYIVADSDLQVALRTSNRTGGRSMRFVRA 300
           NNGGVRGLEKGEGETSSSSLGARRCYSMGSYQYIVADSDLQVALRTSNRTGGRSMRFVRA
Sbjct: 241 NNGGVRGLEKGEGETSSSSLGARRCYSMGSYQYIVADSDLQVALRTSNRTGGRSMRFVRA 300

Query: 301 RDSQTGNSLIDDDAEGKKINIGSKNESFSVSKIWLWSKKARQPIGSDVHLRAN 353
           RDSQTGNSLIDDDAEGKKINIGSKNESFSVSKIWLWSKKARQPIGSDVHLRAN
Sbjct: 301 RDSQTGNSLIDDDAEGKKINIGSKNESFSVSKIWLWSKKARQPIGSDVHLRAN 353

BLAST of MC09g1748 vs. NCBI nr
Match: XP_038893199.1 (RING-H2 finger protein ATL46-like [Benincasa hispida])

HSP 1 Score: 563 bits (1450), Expect = 1.15e-199
Identity = 298/364 (81.87%), Postives = 322/364 (88.46%), Query Frame = 0

Query: 1   MSFLHPQTRVFLVTPSISPLH--SSSPLAY-----QKQPTPPTSSSSKISPAVLLVIVIL 60
           MS +HPQTR FLV+PS SPL   SSS LAY     +++P PPTSSSSKISPAVLLVI+IL
Sbjct: 1   MSSVHPQTRDFLVSPSTSPLQPSSSSSLAYNSDYLKQEPIPPTSSSSKISPAVLLVIIIL 60

Query: 61  AILFFISGILHFLVRFLVKQRSPSS-ISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLD 120
           A+  FISG+LH LVR LVKQRS SS ISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLD
Sbjct: 61  ALFLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLD 120

Query: 121 QAFIDALPVFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNS 180
           QAFIDALPVFLY EIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNS
Sbjct: 121 QAFIDALPVFLYKEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNS 180

Query: 181 TCPLCRGTLYSPGLAIENPIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKRVFSI 240
           TCPLCRGTL+SP LAIENP++GFE+SE TE S EDGR G+PTA K  E+++ GE+RVFSI
Sbjct: 181 TCPLCRGTLHSPVLAIENPVYGFEESEETEVSTEDGRFGIPTAHKASESEIIGERRVFSI 240

Query: 241 RLGKFRNLNNGGVRGLEKGEGETSSSSLGARRCYSMGSYQYIVADSDLQVALRTSNRTGG 300
           RLGKFRNLNNGG+RGLEK EGETSSSSL ARRCYSMGSYQYIVADS+LQVALRTSNR GG
Sbjct: 241 RLGKFRNLNNGGLRGLEKEEGETSSSSLSARRCYSMGSYQYIVADSELQVALRTSNRNGG 300

Query: 301 RSMRFVRA-RDSQTGNSLIDDD--AEGKKINIGSKNESFSVSKIWLWSKKARQPIGSDVH 353
            SMRFV+  RDSQ GN  IDDD  A+GKKINIGSKNESFSVSKIWLWSKK+RQP GSD H
Sbjct: 301 GSMRFVKVIRDSQIGNCPIDDDDDADGKKINIGSKNESFSVSKIWLWSKKSRQPSGSDTH 360

BLAST of MC09g1748 vs. NCBI nr
Match: XP_023513313.1 (RING-H2 finger protein ATL46-like [Cucurbita pepo subsp. pepo] >XP_023513320.1 RING-H2 finger protein ATL46-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 545 bits (1404), Expect = 8.05e-193
Identity = 301/365 (82.47%), Postives = 316/365 (86.58%), Query Frame = 0

Query: 1   MSFL-HPQTRVFLVTPSISPLHSSSPLAY----QKQPTPPTSSSSKISPAVLLVIVILAI 60
           MSF+ HPQTR  LV+PSI+PL  SS LAY    QKQ  PP+SSSSKISPAVLLVIVILA+
Sbjct: 1   MSFVVHPQTRDILVSPSIAPLQPSSSLAYNSDYQKQSAPPSSSSSKISPAVLLVIVILAL 60

Query: 61  LFFISGILHFLVRFLVKQRS-PSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQA 120
           LFFISG+LH LVR LVKQRS PSSI ESNSNRF EMS+SSAFQRQLQQLFHLHDSGLDQA
Sbjct: 61  LFFISGLLHLLVRLLVKQRSSPSSIPESNSNRFLEMSDSSAFQRQLQQLFHLHDSGLDQA 120

Query: 121 FIDALPVFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTC 180
           FIDALPVFLY EIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTC
Sbjct: 121 FIDALPVFLYKEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTC 180

Query: 181 PLCRGTLYSPGLAIENPIFGFEDSEGTEGSAEDGR-IGVPTAQKPPENDMYGEKRVFSIR 240
           PLCRGTLYSPGLAIENP++GFEDSE TE S EDGR I +PTAQK PE+D+ GEKRVFSIR
Sbjct: 181 PLCRGTLYSPGLAIENPVYGFEDSEETEESTEDGRRISIPTAQKAPESDINGEKRVFSIR 240

Query: 241 LGKFRNLN-NGGVRGLEKGEGETSSSS--LGARRCYSMGSYQYIVADSDLQVALRTSNRT 300
           LGKFRNLN NGGVRGLEKGEGETSSSS  LGARRCYSMGSYQYIVADS+LQVAL TSN  
Sbjct: 241 LGKFRNLNSNGGVRGLEKGEGETSSSSSSLGARRCYSMGSYQYIVADSELQVALSTSN-- 300

Query: 301 GGRSMRFVRARDSQTGNSLIDDD-AEGKKINIGSKNESFSVSKIWLWSKKARQPIGSDVH 353
                     RDSQTGN  +DDD AEGKKI IGSKNESFSVSKIWLWSKK RQP G+D H
Sbjct: 301 ---------LRDSQTGNCSVDDDNAEGKKIKIGSKNESFSVSKIWLWSKKGRQPSGADTH 354

BLAST of MC09g1748 vs. NCBI nr
Match: XP_008446237.1 (PREDICTED: RING-H2 finger protein ATL46 [Cucumis melo])

HSP 1 Score: 545 bits (1403), Expect = 1.37e-192
Identity = 297/359 (82.73%), Postives = 317/359 (88.30%), Query Frame = 0

Query: 1   MSFLHPQTRVFLVTPSISPLHSSS---PLAYQK-QPTPP-TSSSSKISPAVLLVIVILAI 60
           MS +HPQTR FLV+PSISPLHSSS    + YQK +PTPP TSSSSKISPAVLLVIVILA+
Sbjct: 1   MSLIHPQTRDFLVSPSISPLHSSSLAYDIDYQKLEPTPPSTSSSSKISPAVLLVIVILAL 60

Query: 61  LFFISGILHFLVRFLVKQRSPSS-ISESNSNRFPEMSESS-AFQRQLQQLFHLHDSGLDQ 120
             FISG+LH LVR LVKQRS SS ISESNSNRFPE+SESS AFQRQLQQLFHLHDSGLDQ
Sbjct: 61  FLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPELSESSSAFQRQLQQLFHLHDSGLDQ 120

Query: 121 AFIDALPVFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNST 180
           AFIDALPVFLY EIVGLKEPFDCAVCLCEFSELDKLRLLP+CSHAFHIDCIDTWLLSNST
Sbjct: 121 AFIDALPVFLYKEIVGLKEPFDCAVCLCEFSELDKLRLLPSCSHAFHIDCIDTWLLSNST 180

Query: 181 CPLCRGTLY--SPGLAIENPIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKRVFS 240
           CPLCRGTL+  SP LAIENP++GFEDSE TE S E+GR G+ TAQK PE+D+ GEKRVFS
Sbjct: 181 CPLCRGTLHIQSPVLAIENPVYGFEDSEETEESTENGRFGILTAQKAPESDIIGEKRVFS 240

Query: 241 IRLGKFRNLNNGGVRGLEKGEGETSSSSLGARRCYSMGSYQYIVADSDLQVALRTSNRTG 300
           IRLGKFRNLNNGG+RGLEKGEGETSSSSL ARRCYSMGSYQYIVA+S+LQVAL TSNR  
Sbjct: 241 IRLGKFRNLNNGGLRGLEKGEGETSSSSLNARRCYSMGSYQYIVAESELQVALHTSNRIS 300

Query: 301 GRSMRFVRA-RDSQTGNSLID---DDAEGKKINIGSKNESFSVSKIWLWSKKARQPIGS 346
           G SMRFV+  RDSQTGN  ID   DDAE KKINIGSKNESFSVSKIWLWSKK+RQP  S
Sbjct: 301 GGSMRFVKVIRDSQTGNCPIDHDDDDAERKKINIGSKNESFSVSKIWLWSKKSRQPCSS 359

BLAST of MC09g1748 vs. NCBI nr
Match: XP_022989163.1 (RING-H2 finger protein ATL46-like [Cucurbita maxima] >XP_022989171.1 RING-H2 finger protein ATL46-like [Cucurbita maxima])

HSP 1 Score: 541 bits (1395), Expect = 1.82e-191
Identity = 299/364 (82.14%), Postives = 314/364 (86.26%), Query Frame = 0

Query: 1   MSFL-HPQTRVFLVTPSISPLHSSSPLAYQ----KQPTPPTSSSSKISPAVLLVIVILAI 60
           MSF+ HPQTR  LV+PSI+PL  SS LAY     KQ  PP+SSSSKISPAVLLVIVILA+
Sbjct: 1   MSFVVHPQTRDILVSPSIAPLQPSSSLAYNSDYHKQSAPPSSSSSKISPAVLLVIVILAL 60

Query: 61  LFFISGILHFLVRFLVKQRS-PSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQA 120
           LFFISG+LH LVR LVKQRS PSSI ESNSNRF EMS+SSAFQRQLQQLFHLHDSGLDQA
Sbjct: 61  LFFISGLLHLLVRLLVKQRSSPSSIPESNSNRFLEMSDSSAFQRQLQQLFHLHDSGLDQA 120

Query: 121 FIDALPVFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTC 180
           FIDALPVFLY EIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTC
Sbjct: 121 FIDALPVFLYKEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTC 180

Query: 181 PLCRGTLYSPGLAIENPIFGFEDSEGTEGSAEDGR-IGVPTAQKPPENDMYGEKRVFSIR 240
           PLCRGTLYSPGLAIENP++GFEDSE TE S EDGR I +PTAQK PE+D+ GEKRVFSIR
Sbjct: 181 PLCRGTLYSPGLAIENPVYGFEDSEETEESTEDGRRISIPTAQKAPESDINGEKRVFSIR 240

Query: 241 LGKFRNLN-NGGVRGLEKGEGETSSSS-LGARRCYSMGSYQYIVADSDLQVALRTSNRTG 300
           LGKFRNLN NGGVRGLEKGEGETSSSS LGARRCYSMGSYQYIVADS+LQVAL TSN   
Sbjct: 241 LGKFRNLNSNGGVRGLEKGEGETSSSSSLGARRCYSMGSYQYIVADSELQVALSTSN--- 300

Query: 301 GRSMRFVRARDSQTGNSLIDDD-AEGKKINIGSKNESFSVSKIWLWSKKARQPIGSDVH- 353
                    RDS TGN  +DDD AEGKKI IGSKNESFSVSKIWLWSKK RQP G+D H 
Sbjct: 301 --------LRDSHTGNCSVDDDNAEGKKIKIGSKNESFSVSKIWLWSKKGRQPSGADTHV 353

BLAST of MC09g1748 vs. ExPASy TrEMBL
Match: A0A6J1DCR2 (RING-H2 finger protein ATL47 OS=Momordica charantia OX=3673 GN=LOC111019155 PE=4 SV=1)

HSP 1 Score: 694 bits (1792), Expect = 2.92e-252
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0

Query: 1   MSFLHPQTRVFLVTPSISPLHSSSPLAYQKQPTPPTSSSSKISPAVLLVIVILAILFFIS 60
           MSFLHPQTRVFLVTPSISPLHSSSPLAYQKQPTPPTSSSSKISPAVLLVIVILAILFFIS
Sbjct: 1   MSFLHPQTRVFLVTPSISPLHSSSPLAYQKQPTPPTSSSSKISPAVLLVIVILAILFFIS 60

Query: 61  GILHFLVRFLVKQRSPSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALP 120
           GILHFLVRFLVKQRSPSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALP
Sbjct: 61  GILHFLVRFLVKQRSPSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALP 120

Query: 121 VFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGT 180
           VFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGT
Sbjct: 121 VFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGT 180

Query: 181 LYSPGLAIENPIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKRVFSIRLGKFRNL 240
           LYSPGLAIENPIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKRVFSIRLGKFRNL
Sbjct: 181 LYSPGLAIENPIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKRVFSIRLGKFRNL 240

Query: 241 NNGGVRGLEKGEGETSSSSLGARRCYSMGSYQYIVADSDLQVALRTSNRTGGRSMRFVRA 300
           NNGGVRGLEKGEGETSSSSLGARRCYSMGSYQYIVADSDLQVALRTSNRTGGRSMRFVRA
Sbjct: 241 NNGGVRGLEKGEGETSSSSLGARRCYSMGSYQYIVADSDLQVALRTSNRTGGRSMRFVRA 300

Query: 301 RDSQTGNSLIDDDAEGKKINIGSKNESFSVSKIWLWSKKARQPIGSDVHLRAN 353
           RDSQTGNSLIDDDAEGKKINIGSKNESFSVSKIWLWSKKARQPIGSDVHLRAN
Sbjct: 301 RDSQTGNSLIDDDAEGKKINIGSKNESFSVSKIWLWSKKARQPIGSDVHLRAN 353

BLAST of MC09g1748 vs. ExPASy TrEMBL
Match: A0A1S3BE30 (RING-H2 finger protein ATL46 OS=Cucumis melo OX=3656 GN=LOC103489027 PE=4 SV=1)

HSP 1 Score: 545 bits (1403), Expect = 6.64e-193
Identity = 297/359 (82.73%), Postives = 317/359 (88.30%), Query Frame = 0

Query: 1   MSFLHPQTRVFLVTPSISPLHSSS---PLAYQK-QPTPP-TSSSSKISPAVLLVIVILAI 60
           MS +HPQTR FLV+PSISPLHSSS    + YQK +PTPP TSSSSKISPAVLLVIVILA+
Sbjct: 1   MSLIHPQTRDFLVSPSISPLHSSSLAYDIDYQKLEPTPPSTSSSSKISPAVLLVIVILAL 60

Query: 61  LFFISGILHFLVRFLVKQRSPSS-ISESNSNRFPEMSESS-AFQRQLQQLFHLHDSGLDQ 120
             FISG+LH LVR LVKQRS SS ISESNSNRFPE+SESS AFQRQLQQLFHLHDSGLDQ
Sbjct: 61  FLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPELSESSSAFQRQLQQLFHLHDSGLDQ 120

Query: 121 AFIDALPVFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNST 180
           AFIDALPVFLY EIVGLKEPFDCAVCLCEFSELDKLRLLP+CSHAFHIDCIDTWLLSNST
Sbjct: 121 AFIDALPVFLYKEIVGLKEPFDCAVCLCEFSELDKLRLLPSCSHAFHIDCIDTWLLSNST 180

Query: 181 CPLCRGTLY--SPGLAIENPIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKRVFS 240
           CPLCRGTL+  SP LAIENP++GFEDSE TE S E+GR G+ TAQK PE+D+ GEKRVFS
Sbjct: 181 CPLCRGTLHIQSPVLAIENPVYGFEDSEETEESTENGRFGILTAQKAPESDIIGEKRVFS 240

Query: 241 IRLGKFRNLNNGGVRGLEKGEGETSSSSLGARRCYSMGSYQYIVADSDLQVALRTSNRTG 300
           IRLGKFRNLNNGG+RGLEKGEGETSSSSL ARRCYSMGSYQYIVA+S+LQVAL TSNR  
Sbjct: 241 IRLGKFRNLNNGGLRGLEKGEGETSSSSLNARRCYSMGSYQYIVAESELQVALHTSNRIS 300

Query: 301 GRSMRFVRA-RDSQTGNSLID---DDAEGKKINIGSKNESFSVSKIWLWSKKARQPIGS 346
           G SMRFV+  RDSQTGN  ID   DDAE KKINIGSKNESFSVSKIWLWSKK+RQP  S
Sbjct: 301 GGSMRFVKVIRDSQTGNCPIDHDDDDAERKKINIGSKNESFSVSKIWLWSKKSRQPCSS 359

BLAST of MC09g1748 vs. ExPASy TrEMBL
Match: A0A6J1JJB0 (RING-H2 finger protein ATL46-like OS=Cucurbita maxima OX=3661 GN=LOC111486314 PE=4 SV=1)

HSP 1 Score: 541 bits (1395), Expect = 8.81e-192
Identity = 299/364 (82.14%), Postives = 314/364 (86.26%), Query Frame = 0

Query: 1   MSFL-HPQTRVFLVTPSISPLHSSSPLAYQ----KQPTPPTSSSSKISPAVLLVIVILAI 60
           MSF+ HPQTR  LV+PSI+PL  SS LAY     KQ  PP+SSSSKISPAVLLVIVILA+
Sbjct: 1   MSFVVHPQTRDILVSPSIAPLQPSSSLAYNSDYHKQSAPPSSSSSKISPAVLLVIVILAL 60

Query: 61  LFFISGILHFLVRFLVKQRS-PSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQA 120
           LFFISG+LH LVR LVKQRS PSSI ESNSNRF EMS+SSAFQRQLQQLFHLHDSGLDQA
Sbjct: 61  LFFISGLLHLLVRLLVKQRSSPSSIPESNSNRFLEMSDSSAFQRQLQQLFHLHDSGLDQA 120

Query: 121 FIDALPVFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTC 180
           FIDALPVFLY EIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTC
Sbjct: 121 FIDALPVFLYKEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTC 180

Query: 181 PLCRGTLYSPGLAIENPIFGFEDSEGTEGSAEDGR-IGVPTAQKPPENDMYGEKRVFSIR 240
           PLCRGTLYSPGLAIENP++GFEDSE TE S EDGR I +PTAQK PE+D+ GEKRVFSIR
Sbjct: 181 PLCRGTLYSPGLAIENPVYGFEDSEETEESTEDGRRISIPTAQKAPESDINGEKRVFSIR 240

Query: 241 LGKFRNLN-NGGVRGLEKGEGETSSSS-LGARRCYSMGSYQYIVADSDLQVALRTSNRTG 300
           LGKFRNLN NGGVRGLEKGEGETSSSS LGARRCYSMGSYQYIVADS+LQVAL TSN   
Sbjct: 241 LGKFRNLNSNGGVRGLEKGEGETSSSSSLGARRCYSMGSYQYIVADSELQVALSTSN--- 300

Query: 301 GRSMRFVRARDSQTGNSLIDDD-AEGKKINIGSKNESFSVSKIWLWSKKARQPIGSDVH- 353
                    RDS TGN  +DDD AEGKKI IGSKNESFSVSKIWLWSKK RQP G+D H 
Sbjct: 301 --------LRDSHTGNCSVDDDNAEGKKIKIGSKNESFSVSKIWLWSKKGRQPSGADTHV 353

BLAST of MC09g1748 vs. ExPASy TrEMBL
Match: A0A0A0KVN9 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G602200 PE=4 SV=1)

HSP 1 Score: 541 bits (1393), Expect = 2.21e-191
Identity = 296/359 (82.45%), Postives = 315/359 (87.74%), Query Frame = 0

Query: 1   MSFLHPQTRVFLVTPSISPLHSSS---PLAYQK-QPTPP-TSSSSKISPAVLLVIVILAI 60
           MS  HPQTR F V+PSISPLHSSS    + YQK +PTPP TSSSSKISPAVLLVIVILA+
Sbjct: 1   MSLSHPQTRDFPVSPSISPLHSSSLAYDIDYQKLEPTPPSTSSSSKISPAVLLVIVILAL 60

Query: 61  LFFISGILHFLVRFLVKQRSPSS-ISESNSNRFPEMSESS-AFQRQLQQLFHLHDSGLDQ 120
             FISG+LH LVR LVKQRS SS ISESNSNRFP++SESS AFQRQLQQLFHLHDSGLDQ
Sbjct: 61  FLFISGLLHLLVRLLVKQRSSSSSISESNSNRFPDLSESSSAFQRQLQQLFHLHDSGLDQ 120

Query: 121 AFIDALPVFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNST 180
           AFIDALPVFLY EIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNST
Sbjct: 121 AFIDALPVFLYKEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNST 180

Query: 181 CPLCRGTLY--SPGLAIENPIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKRVFS 240
           CPLCRGTL+  SP LAIENP++GFEDSE TE S E+GR G+ TAQK PE+D+ GEKRVFS
Sbjct: 181 CPLCRGTLHIQSPVLAIENPVYGFEDSEETEESTENGRFGILTAQKAPESDIIGEKRVFS 240

Query: 241 IRLGKFRNLNNGGVRGLEKGEGETSSSSLGARRCYSMGSYQYIVADSDLQVALRTSNRTG 300
           IRLGKFRNLNNGG+RGLEKGEGETSSSSL ARRCYSMGSYQYIVA+S+LQVAL TSNR  
Sbjct: 241 IRLGKFRNLNNGGLRGLEKGEGETSSSSLSARRCYSMGSYQYIVAESELQVALHTSNRIS 300

Query: 301 GRSMRFVRA-RDSQTGNSLID---DDAEGKKINIGSKNESFSVSKIWLWSKKARQPIGS 346
           G SMRFV+  RDSQTGN  ID   DDAE KKINIGSKNESFSVSKIWLWSKK+RQP  S
Sbjct: 301 GGSMRFVKVIRDSQTGNCPIDRDDDDAERKKINIGSKNESFSVSKIWLWSKKSRQPCSS 359

BLAST of MC09g1748 vs. ExPASy TrEMBL
Match: A0A6J1FN91 (RING-H2 finger protein ATL46-like OS=Cucurbita moschata OX=3662 GN=LOC111447334 PE=4 SV=1)

HSP 1 Score: 540 bits (1391), Expect = 3.58e-191
Identity = 299/364 (82.14%), Postives = 315/364 (86.54%), Query Frame = 0

Query: 1   MSFL-HPQTRVFLVTPSISPLHSSSPLAY----QKQPTPPTSSSSKISPAVLLVIVILAI 60
           MSF+ HPQTR  LV+PSI+PL  SS LAY    QKQ   P+SSSSKISPAVLLVIVILA+
Sbjct: 1   MSFVVHPQTRDILVSPSIAPLQPSSSLAYNSDYQKQSALPSSSSSKISPAVLLVIVILAL 60

Query: 61  LFFISGILHFLVRFLVKQRS-PSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQA 120
           LFFISG+LH LVR LVKQRS PSSI ESNSNRF EMS+SSAFQRQLQQLFHLHDSGLDQA
Sbjct: 61  LFFISGLLHLLVRLLVKQRSSPSSIPESNSNRFLEMSDSSAFQRQLQQLFHLHDSGLDQA 120

Query: 121 FIDALPVFLYTEIVGLKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTC 180
           FIDALPVFLY EIVGLKEPFDCAVCLCEFSELDKLRLLP+CSHAFHIDCIDTWLLSNSTC
Sbjct: 121 FIDALPVFLYKEIVGLKEPFDCAVCLCEFSELDKLRLLPSCSHAFHIDCIDTWLLSNSTC 180

Query: 181 PLCRGTLYSPGLAIENPIFGFEDSEGTEGSAEDGR-IGVPTAQKPPENDMYGEKRVFSIR 240
           PLCRGTLYSPGLAIENP++GFEDSE TE S EDGR I +PTAQK PE+D+ GEKRVFSIR
Sbjct: 181 PLCRGTLYSPGLAIENPVYGFEDSEETEESTEDGRRISIPTAQKAPESDINGEKRVFSIR 240

Query: 241 LGKFRNLN-NGGVRGLEKGEGETSSSS-LGARRCYSMGSYQYIVADSDLQVALRTSNRTG 300
           LGKFRNLN NGGVRGLEKGEGETSSSS LGARRCYSMGSYQYIVADS+LQVAL TSN   
Sbjct: 241 LGKFRNLNSNGGVRGLEKGEGETSSSSSLGARRCYSMGSYQYIVADSELQVALSTSN--- 300

Query: 301 GRSMRFVRARDSQTGNSLIDDD-AEGKKINIGSKNESFSVSKIWLWSKKARQPIGSDVH- 353
                    RDSQTGN  +DDD AEGKKI IGSKNESFSVSKIWLWSKK RQP G+D H 
Sbjct: 301 --------LRDSQTGNCSVDDDNAEGKKIKIGSKNESFSVSKIWLWSKKGRQPSGADTHV 353

BLAST of MC09g1748 vs. TAIR 10
Match: AT1G23980.1 (RING/U-box superfamily protein )

HSP 1 Score: 336.3 bits (861), Expect = 3.0e-92
Identity = 199/332 (59.94%), Postives = 230/332 (69.28%), Query Frame = 0

Query: 17  ISPLHSSSPLAYQKQPTPPTSSSS-----KISPAVLLVIVILAILFFISGILHFLVR-FL 76
           ISP  + SP+    Q T  +SSSS     +ISP +L +IV+L+++FFI  ILH LVR +L
Sbjct: 19  ISPSSAPSPITLNHQLTDSSSSSSSGGNNRISPIILFIIVLLSVIFFICSILHLLVRYYL 78

Query: 77  VKQRSPSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYTEIVGL 136
            K+RS  S S + SN+ PE S+S  +QRQLQQLFHLHDSGLDQA IDALPVFLY EI G 
Sbjct: 79  KKKRSNLSSSPNESNQNPEFSDSDTYQRQLQQLFHLHDSGLDQALIDALPVFLYKEIKGT 138

Query: 137 KEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTLYSPGLAIEN 196
           KEPFDCAVCLCEFSE DKLRLLP CSHAFHIDCIDTWLLSNSTCPLCRGTL+S G   E 
Sbjct: 139 KEPFDCAVCLCEFSEDDKLRLLPNCSHAFHIDCIDTWLLSNSTCPLCRGTLFSLGHQFEY 198

Query: 197 PIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKRVFSIRLGKFRN---LNNGGVRG 256
           P F F    G +G    G + V   QKP EN++   KRVFS+RLGKFR+   +NNG V  
Sbjct: 199 PDFNFGFFAGDDGG---GGVRVSPVQKPAENEI--GKRVFSVRLGKFRSSNIVNNGEV-- 258

Query: 257 LEKGEGETSSSSLGARRCYSMGSYQYIVADSDLQVALRTSNRTGGRSMRFVRARDSQTGN 316
           +  G GETSSSSL  RRC+SMGSYQYIVA+SDL VAL   N  G ++             
Sbjct: 259 VVGGGGETSSSSLDNRRCFSMGSYQYIVAESDLVVAL-CPNNEGLKN------------- 318

Query: 317 SLIDDDAEGKKINIGSKNESFSVSKIWLWSKK 340
              + D EGKKIN+ SK ESFSVSKIW WS K
Sbjct: 319 ---NKDVEGKKINMRSKGESFSVSKIWQWSNK 326

BLAST of MC09g1748 vs. TAIR 10
Match: AT5G40250.1 (RING/U-box superfamily protein )

HSP 1 Score: 332.0 bits (850), Expect = 5.7e-91
Identity = 192/320 (60.00%), Postives = 229/320 (71.56%), Query Frame = 0

Query: 34  PPTSSSSKISPAVLLVIVILAILFFISGILHFLVRFLVKQRSPSSISESNSNRFPEMSES 93
           PP SS ++ISPAVL VIVILA+LFFISG+LH LVRFL+K   PS+ + S SNRFPE+S S
Sbjct: 33  PPPSSGNRISPAVLFVIVILAVLFFISGLLHLLVRFLIKH--PSATASSRSNRFPEISTS 92

Query: 94  SAFQRQLQQLFHLHDSGLDQAFIDALPVFLYTEIVG----------LKEPFDCAVCLCEF 153
            A QRQLQQLFHL+DSGLDQAFIDALPVF Y EIVG           +EPFDCAVCLCEF
Sbjct: 93  DALQRQLQQLFHLNDSGLDQAFIDALPVFHYKEIVGSAGGGGGNGAAQEPFDCAVCLCEF 152

Query: 154 SELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTLYSPGLAIENPIFGFED-SEGTE 213
           SE DKLRLLP CSHAFH++CIDTWL SNSTCPLCRGTL+SPG ++ENP+F F+D  E  E
Sbjct: 153 SEKDKLRLLPMCSHAFHLNCIDTWLQSNSTCPLCRGTLFSPGFSMENPMFDFDDIREDEE 212

Query: 214 GSAEDGRIGVPTAQKPPE-NDMYGEKRVFSIRLGKFRNLNN-GGVRGLE-KGEGETSSSS 273
           G  E+G      +QK  E  ++  EK V  +RLGKF+ L+N G  +G +    GETSSS+
Sbjct: 213 GVTENG------SQKTMEIQEIVVEKGVLPVRLGKFKRLDNVGNGQGQDVVAGGETSSSN 272

Query: 274 LGARRCYSMGSYQYIVADSDLQVALRTSNRTGGRSMRFVRARDSQTGNSLIDDDAEGKKI 333
           L ARRC+SMGSYQYI+ +S+L+V            ++       QTGNS  +D+   KKI
Sbjct: 273 LDARRCFSMGSYQYILGNSELKVPFANDRLP---RLKPQDKESEQTGNSSSEDN---KKI 332

Query: 334 NIGSKNESFSVSKIWLWSKK 340
           N  +K ESFSVSKIWLW KK
Sbjct: 333 NTVAKGESFSVSKIWLWPKK 338

BLAST of MC09g1748 vs. TAIR 10
Match: AT4G30400.1 (RING/U-box superfamily protein )

HSP 1 Score: 223.4 bits (568), Expect = 2.9e-58
Identity = 162/396 (40.91%), Postives = 215/396 (54.29%), Query Frame = 0

Query: 1   MSFLHPQTRVFLVTPSISPLHSSSPLAYQKQPTPPTSSSSKISPAVLLVIVILAILFFIS 60
           M ++ P+ +      S S L    PL+ +         +SKISP++LL+I+IL+I+FFIS
Sbjct: 8   MKWVFPEIKTTQNFLSPSSLPQEPPLSLRSSAN--FDLNSKISPSILLIIIILSIIFFIS 67

Query: 61  GILHFLVRFLVKQRSPSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALP 120
           G+LH LVRFL        ++ S+ +R       +A Q QLQQLFHLHDSG+DQ+FID LP
Sbjct: 68  GLLHLLVRFL--------LTPSSRDREDYFDNVTALQGQLQQLFHLHDSGVDQSFIDTLP 127

Query: 121 VFLYTEIVGLKE-PFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRG 180
           VF Y  I+GLK  PFDCAVCLCEF   DKLRLLP CSHAFH+DCIDTWLLS+STCPLCR 
Sbjct: 128 VFHYKSIIGLKNYPFDCAVCLCEFETEDKLRLLPKCSHAFHMDCIDTWLLSHSTCPLCRS 187

Query: 181 TLYSPGLAIENP------------------IFGFEDSEGTEGSAED--------GRIG-- 240
           +L S   + ++P                  I G  DS     + +D        G +G  
Sbjct: 188 SLLSDLSSHQDPRSSFLLVLESASDHSSREIGGDRDSAACVAANDDIDVSSAHLGLVGNN 247

Query: 241 --VPTAQKPPENDMY---------GEKRVFSIRLGKFRNLNNGGVRGLEKGEGETSSSSL 300
               T       D Y         G+   FS++LGKFRN++ G   G        +SSSL
Sbjct: 248 DLGSTRIDSGHGDQYLDGELGGSVGKVVPFSVKLGKFRNIDIG--EGTSSNNNIGNSSSL 307

Query: 301 GARRCYSMGSYQYIV-ADSDLQVALRT-----------SNRTGGRSMRF---VRARDSQT 342
             RRC+SMGSY+YI+  ++ L+V + T            +RT      F    R + S +
Sbjct: 308 DERRCFSMGSYEYIMDEETTLKVHVSTKKQSSKNRGLPGHRTAMSECGFDPTGRLKFSGS 367

BLAST of MC09g1748 vs. TAIR 10
Match: AT2G18650.1 (RING/U-box superfamily protein )

HSP 1 Score: 216.5 bits (550), Expect = 3.5e-56
Identity = 147/345 (42.61%), Postives = 197/345 (57.10%), Query Frame = 0

Query: 25  PLAYQKQPTPPTSSSSKISPAVLLVIVILAILFFISGILHFLVRFLVKQRSPSSISESNS 84
           PL   K  T  ++ +SKI+P +LL+I+IL+I+FFISG+LH LV+FL        ++ S  
Sbjct: 22  PLIPLKSNTSLSNLNSKITPNILLIIIILSIIFFISGLLHILVKFL--------LTPSRE 81

Query: 85  NRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYTEIVGLK-EPFDCAVCLCEF 144
           +R       +A Q QLQQLF+LHDSG+DQ+ ID LPVF Y  IVGLK  PFDC VCLCEF
Sbjct: 82  SREDYFDNVTALQGQLQQLFNLHDSGVDQSLIDTLPVFHYKSIVGLKISPFDCPVCLCEF 141

Query: 145 SELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTLYSPGLAIEN------------- 204
              DKLRLLP CSHAFH++CIDTWLLS+STCPLCR  L S   +  N             
Sbjct: 142 ETEDKLRLLPKCSHAFHVECIDTWLLSHSTCPLCRSNLLSGFSSHHNLSSSYLLVLESEQ 201

Query: 205 ------PIFGFEDSEG--TEGSAEDGRIGVPTAQKPPENDMYG--EKRV-FSIRLGKFRN 264
                 P+       G      +E  RI        P+ DM G  EK V   ++LGKFRN
Sbjct: 202 SSRDMVPVLESNSQLGYDVNNDSESTRIRSGRKSCDPDGDMDGLDEKVVPLEVKLGKFRN 261

Query: 265 LNNGGVRGLEKGEG-ETSSSSLGARRCYSMGSYQYIV-ADSDLQVALRTSNRTGGRSMRF 324
           +++ G    +K      +S ++  RRC SMGSY+YI+  ++ L+V + T   +G   +  
Sbjct: 262 IDHVGEGSDQKKNSISGNSKNVDGRRCLSMGSYEYIMDQEATLKVHVSTKKLSGKDRVPS 321

Query: 325 VRARDSQTGNSLIDDDAEGKKINIGSKNESFSVSKIWLWSKKARQ 343
            R   S+ G    D   +G + ++  + ESFS+SKIWL  KK +Q
Sbjct: 322 HRTVMSECG---FDPTVKGIEKSV-VERESFSLSKIWLRGKKEKQ 354

BLAST of MC09g1748 vs. TAIR 10
Match: AT3G48030.1 (hypoxia-responsive family protein / zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 187.2 bits (474), Expect = 2.3e-47
Identity = 126/291 (43.30%), Postives = 164/291 (56.36%), Query Frame = 0

Query: 14  TPSISPLHSSS---PLAYQKQPTPPTSSSSKISPAVLLVIVILAILFFISGILHFLVRFL 73
           T ++SP  SS+    L  QK P     SSS I   + LVI  LA++    G+L+ + +FL
Sbjct: 90  TEALSPKSSSAATLTLMNQKDP-----SSSSIVSVLCLVISGLALIIVFLGVLYLIFKFL 149

Query: 74  VKQRSPSSISESNSNRFPEMSESSAFQRQLQQLFHLHDSGLDQAFIDALPVFLYTEI-VG 133
            K  +   I   N N  P++   S+   QLQ LF LHDSGLDQ  IDALPVFLY  + + 
Sbjct: 150 RKSSTLFPIPHFNYN--PDLFSFSS--PQLQHLFFLHDSGLDQTAIDALPVFLYGNVTIS 209

Query: 134 LKEPFDCAVCLCEFSELDKLRLLPTCSHAFHIDCIDTWLLSNSTCPLCRGTL------YS 193
           L++PFDCAVCL EFS+ DKLRLLP CSHAFH+ CIDTWLLSNSTCPLCR +L      Y+
Sbjct: 210 LEQPFDCAVCLNEFSDTDKLRLLPVCSHAFHLHCIDTWLLSNSTCPLCRRSLSTSNVCYN 269

Query: 194 PGLAIENPIFGFEDSEGTEGSAEDGRIGVPTAQKPPENDMYGEKRVFSIRLGKFRNLNNG 253
               +  P+ G +         +DG+  +              KRVFS+RLG+F++ N  
Sbjct: 270 HSETLVAPLSGHQ-------QVDDGKASL-------------AKRVFSVRLGRFKSTNE- 329

Query: 254 GVRGLEKGEGETSSSSLGA---RRCYSMGSYQYIVADSDLQVALRTSNRTG 292
                 + +       +G    RRCYSMG+ QY+V D D  VAL +S R G
Sbjct: 330 -----SQSQRHDVKDEIGVRMPRRCYSMGTQQYLVCDQDFVVALSSSPREG 345

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GW384.3e-9159.94RING-H2 finger protein ATL47 OS=Arabidopsis thaliana OX=3702 GN=ATL47 PE=2 SV=1[more]
Q9FL078.1e-9060.00RING-H2 finger protein ATL46 OS=Arabidopsis thaliana OX=3702 GN=ATL46 PE=2 SV=1[more]
Q940Q44.0e-5740.91RING-H2 finger protein ATL13 OS=Arabidopsis thaliana OX=3702 GN=ATL13 PE=2 SV=2[more]
Q9ZV534.9e-5542.61Putative RING-H2 finger protein ATL49 OS=Arabidopsis thaliana OX=3702 GN=ATL49 P... [more]
Q7X8433.2e-4643.30RING-H2 finger protein ATL48 OS=Arabidopsis thaliana OX=3702 GN=ATL48 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_022151161.16.02e-252100.00RING-H2 finger protein ATL47 [Momordica charantia] >XP_022151162.1 RING-H2 finge... [more]
XP_038893199.11.15e-19981.87RING-H2 finger protein ATL46-like [Benincasa hispida][more]
XP_023513313.18.05e-19382.47RING-H2 finger protein ATL46-like [Cucurbita pepo subsp. pepo] >XP_023513320.1 R... [more]
XP_008446237.11.37e-19282.73PREDICTED: RING-H2 finger protein ATL46 [Cucumis melo][more]
XP_022989163.11.82e-19182.14RING-H2 finger protein ATL46-like [Cucurbita maxima] >XP_022989171.1 RING-H2 fin... [more]
Match NameE-valueIdentityDescription
A0A6J1DCR22.92e-252100.00RING-H2 finger protein ATL47 OS=Momordica charantia OX=3673 GN=LOC111019155 PE=4... [more]
A0A1S3BE306.64e-19382.73RING-H2 finger protein ATL46 OS=Cucumis melo OX=3656 GN=LOC103489027 PE=4 SV=1[more]
A0A6J1JJB08.81e-19282.14RING-H2 finger protein ATL46-like OS=Cucurbita maxima OX=3661 GN=LOC111486314 PE... [more]
A0A0A0KVN92.21e-19182.45RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G602200 P... [more]
A0A6J1FN913.58e-19182.14RING-H2 finger protein ATL46-like OS=Cucurbita moschata OX=3662 GN=LOC111447334 ... [more]
Match NameE-valueIdentityDescription
AT1G23980.13.0e-9259.94RING/U-box superfamily protein [more]
AT5G40250.15.7e-9160.00RING/U-box superfamily protein [more]
AT4G30400.12.9e-5840.91RING/U-box superfamily protein [more]
AT2G18650.13.5e-5642.61RING/U-box superfamily protein [more]
AT3G48030.12.3e-4743.30hypoxia-responsive family protein / zinc finger (C3HC4-type RING finger) family ... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 136..177
e-value: 1.5E-6
score: 37.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 135..178
e-value: 3.7E-12
score: 46.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 136..178
score: 12.297489
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 96..183
e-value: 6.4E-20
score: 72.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 198..222
NoneNo IPR availablePANTHERPTHR45768:SF54RING-H2 FINGER PROTEIN ATL47-RELATEDcoord: 21..344
NoneNo IPR availablePANTHERPTHR45768E3 UBIQUITIN-PROTEIN LIGASE RNF13-LIKEcoord: 21..344
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 135..178
e-value: 5.72253E-20
score: 80.0385
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 115..184

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g1748.1MC09g1748.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0031625 ubiquitin protein ligase binding