MC09g1678 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g1678
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionprotein PHYTOCHROME KINASE SUBSTRATE 1-like
LocationMC09: 22159640 .. 22160917 (+)
RNA-Seq ExpressionMC09g1678
SyntenyMC09g1678
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAGGGTGAGCCTGCCGCCACCTGTTTGTGACACAAACCTCTCGCAGAGGTTGTCCTTCGAGAACAACAATAATAACAACAACCACCTTCGGGAAGCCTCTTTCTCTTCCTACTTGAACCATGCTGAGAAAGAATTTGTGCGCAAACTCGCTGAAGCGGCTCGAGATCGGCCCCAAGAGGAGGATGGGGATATTGAAGTTTTTGGAGCAGAGAAATACTTCAATGGGGGAATGGACAACCCAAATCCTCTGAAATTACGCCCCAGGAGACAGCAGTTTAAAAAGGCAGAACAGATGAATGTTCACCAAAAGAAGCCAAAAACAAAAATTAGCCGCTCTGGAACTCCAAGTATCCGTTCAGAGTCCAGTTGGAATAGCCAAAATACATTGTTGCAACGGAGAAATCATTCAGGCCCAGGGGAGAGCAAGAAGAAAAATAAATCCTCATTAAAACCAAAGGGGTTTCTCTATAAATGCTGTGGCTGCCACAAAGACTCAGTGGAAGAAGTTCATTCATCCAATAACACCAAAACAAATCCGAAACAAGAACTGGTGAGAAGTGGGTTAAATTTCACATTTCGAAAATCGACCTCGAAATTAGCGGAGCCCAAAAACTTACCCCTGAAAATGAAAATTCAAGTAGAGGAACAGCAAGCGCGCAGGTCAATTGAGGTTTTTGGGTCTTCCCTGAAGGCGCAAAGTGTGGAGACTGTGAGCCTGAACCACCTAGAGAGGAAGCTGTCGATGCTGTCTTGGAACGACATCGGTCCAAGAGTGAAGGAATCAGATTCGAATATGTCCGTGTCCGCCACTGATGATTCGATTCTAATCGACAATGGGGATGCGGAAAGCGATGCAAGTTCGGATCTTTTCGAGATAGAGAGCTTAATGGGGAACCCCAATCCGTTTCTGGTGAGACAGGGCTCTGATTGCACGGATTGTGTCACGCCCACCACTTGTTACGCGCCGAGCGAGGCCAGCATCGAGTGGAGTGTCGTCACCGCCAGCGCCACCGACTACGACGAACGGCGCCTCTCCACCACCAGCCCAACCGCCGCCGCCGAAGAGATTCAGAGGCGGCGCTCCAATCTTCTGATGGGGTGTAGAAGTAAGAAGGCGGCGGGAGATGCGTACGGTTCCGAGGCAGAAGTTTGCCGGCGGGCGGAGGCGTTTGGGCGGATGACTAGGTTCGAAGCTGAGACGAAGGTTATGGGTTTTGTTGGCACGAACTCGATTAGTCGTCGGTATTCGCCCCAAATGTCAAATATTTTGTAT

mRNA sequence

ATGGGAAGGGTGAGCCTGCCGCCACCTGTTTGTGACACAAACCTCTCGCAGAGGTTGTCCTTCGAGAACAACAATAATAACAACAACCACCTTCGGGAAGCCTCTTTCTCTTCCTACTTGAACCATGCTGAGAAAGAATTTGTGCGCAAACTCGCTGAAGCGGCTCGAGATCGGCCCCAAGAGGAGGATGGGGATATTGAAGTTTTTGGAGCAGAGAAATACTTCAATGGGGGAATGGACAACCCAAATCCTCTGAAATTACGCCCCAGGAGACAGCAGTTTAAAAAGGCAGAACAGATGAATGTTCACCAAAAGAAGCCAAAAACAAAAATTAGCCGCTCTGGAACTCCAAGTATCCGTTCAGAGTCCAGTTGGAATAGCCAAAATACATTGTTGCAACGGAGAAATCATTCAGGCCCAGGGGAGAGCAAGAAGAAAAATAAATCCTCATTAAAACCAAAGGGGTTTCTCTATAAATGCTGTGGCTGCCACAAAGACTCAGTGGAAGAAGTTCATTCATCCAATAACACCAAAACAAATCCGAAACAAGAACTGGTGAGAAGTGGGTTAAATTTCACATTTCGAAAATCGACCTCGAAATTAGCGGAGCCCAAAAACTTACCCCTGAAAATGAAAATTCAAGTAGAGGAACAGCAAGCGCGCAGGTCAATTGAGGTTTTTGGGTCTTCCCTGAAGGCGCAAAGTGTGGAGACTGTGAGCCTGAACCACCTAGAGAGGAAGCTGTCGATGCTGTCTTGGAACGACATCGGTCCAAGAGTGAAGGAATCAGATTCGAATATGTCCGTGTCCGCCACTGATGATTCGATTCTAATCGACAATGGGGATGCGGAAAGCGATGCAAGTTCGGATCTTTTCGAGATAGAGAGCTTAATGGGGAACCCCAATCCGTTTCTGGTGAGACAGGGCTCTGATTGCACGGATTGTGTCACGCCCACCACTTGTTACGCGCCGAGCGAGGCCAGCATCGAGTGGAGTGTCGTCACCGCCAGCGCCACCGACTACGACGAACGGCGCCTCTCCACCACCAGCCCAACCGCCGCCGCCGAAGAGATTCAGAGGCGGCGCTCCAATCTTCTGATGGGGTGTAGAAGTAAGAAGGCGGCGGGAGATGCGTACGGTTCCGAGGCAGAAGTTTGCCGGCGGGCGGAGGCGTTTGGGCGGATGACTAGGTTCGAAGCTGAGACGAAGGTTATGGGTTTTGTTGGCACGAACTCGATTAGTCGTCGGTATTCGCCCCAAATGTCAAATATTTTGTAT

Coding sequence (CDS)

ATGGGAAGGGTGAGCCTGCCGCCACCTGTTTGTGACACAAACCTCTCGCAGAGGTTGTCCTTCGAGAACAACAATAATAACAACAACCACCTTCGGGAAGCCTCTTTCTCTTCCTACTTGAACCATGCTGAGAAAGAATTTGTGCGCAAACTCGCTGAAGCGGCTCGAGATCGGCCCCAAGAGGAGGATGGGGATATTGAAGTTTTTGGAGCAGAGAAATACTTCAATGGGGGAATGGACAACCCAAATCCTCTGAAATTACGCCCCAGGAGACAGCAGTTTAAAAAGGCAGAACAGATGAATGTTCACCAAAAGAAGCCAAAAACAAAAATTAGCCGCTCTGGAACTCCAAGTATCCGTTCAGAGTCCAGTTGGAATAGCCAAAATACATTGTTGCAACGGAGAAATCATTCAGGCCCAGGGGAGAGCAAGAAGAAAAATAAATCCTCATTAAAACCAAAGGGGTTTCTCTATAAATGCTGTGGCTGCCACAAAGACTCAGTGGAAGAAGTTCATTCATCCAATAACACCAAAACAAATCCGAAACAAGAACTGGTGAGAAGTGGGTTAAATTTCACATTTCGAAAATCGACCTCGAAATTAGCGGAGCCCAAAAACTTACCCCTGAAAATGAAAATTCAAGTAGAGGAACAGCAAGCGCGCAGGTCAATTGAGGTTTTTGGGTCTTCCCTGAAGGCGCAAAGTGTGGAGACTGTGAGCCTGAACCACCTAGAGAGGAAGCTGTCGATGCTGTCTTGGAACGACATCGGTCCAAGAGTGAAGGAATCAGATTCGAATATGTCCGTGTCCGCCACTGATGATTCGATTCTAATCGACAATGGGGATGCGGAAAGCGATGCAAGTTCGGATCTTTTCGAGATAGAGAGCTTAATGGGGAACCCCAATCCGTTTCTGGTGAGACAGGGCTCTGATTGCACGGATTGTGTCACGCCCACCACTTGTTACGCGCCGAGCGAGGCCAGCATCGAGTGGAGTGTCGTCACCGCCAGCGCCACCGACTACGACGAACGGCGCCTCTCCACCACCAGCCCAACCGCCGCCGCCGAAGAGATTCAGAGGCGGCGCTCCAATCTTCTGATGGGGTGTAGAAGTAAGAAGGCGGCGGGAGATGCGTACGGTTCCGAGGCAGAAGTTTGCCGGCGGGCGGAGGCGTTTGGGCGGATGACTAGGTTCGAAGCTGAGACGAAGGTTATGGGTTTTGTTGGCACGAACTCGATTAGTCGTCGGTATTCGCCCCAAATGTCAAATATTTTGTAT

Protein sequence

MGRVSLPPPVCDTNLSQRLSFENNNNNNNHLREASFSSYLNHAEKEFVRKLAEAARDRPQEEDGDIEVFGAEKYFNGGMDNPNPLKLRPRRQQFKKAEQMNVHQKKPKTKISRSGTPSIRSESSWNSQNTLLQRRNHSGPGESKKKNKSSLKPKGFLYKCCGCHKDSVEEVHSSNNTKTNPKQELVRSGLNFTFRKSTSKLAEPKNLPLKMKIQVEEQQARRSIEVFGSSLKAQSVETVSLNHLERKLSMLSWNDIGPRVKESDSNMSVSATDDSILIDNGDAESDASSDLFEIESLMGNPNPFLVRQGSDCTDCVTPTTCYAPSEASIEWSVVTASATDYDERRLSTTSPTAAAEEIQRRRSNLLMGCRSKKAAGDAYGSEAEVCRRAEAFGRMTRFEAETKVMGFVGTNSISRRYSPQMSNILY
Homology
BLAST of MC09g1678 vs. ExPASy Swiss-Prot
Match: Q9SWI1 (Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Arabidopsis thaliana OX=3702 GN=PKS1 PE=1 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 2.5e-29
Identity = 148/427 (34.66%), Postives = 195/427 (45.67%), Query Frame = 0

Query: 4   VSLPPPVCDTNLSQRLSFENNNNNNNHLR-EASFSSYLNHAEKEFV--RKLAEAAR---- 63
           V+L P    T    + SF+   NNN+H     S SSYL+  E   V  +KL E ++    
Sbjct: 2   VTLTPSSAST---PKTSFDFMKNNNSHSSLYVSSSSYLSSKEDALVTTKKLMEPSKTLNM 61

Query: 64  --DRPQEEDGD-------------IEVFGAEKYFNGGMDNPNPLKLRPRRQQFKKAEQMN 123
             +  QEE GD             I VFGAEKYFNG MD+     +        + E+  
Sbjct: 62  SINPKQEEFGDEKKMVKKAPEDPEIGVFGAEKYFNGDMDSDQGSSVLSLTN--PEVERTV 121

Query: 124 VHQKKPKTKISRSGTPSIRSESSWNSQNTLLQRR--NHSGPGESKKKN------KSSLKP 183
           V  K+   K   +GTPS+RSESSWNSQ+ LLQ +  N       +KKN      K +   
Sbjct: 122 VDSKQSAKK--STGTPSVRSESSWNSQSVLLQNKLVNSCNSSFKEKKNSNGQIQKVTNNK 181

Query: 184 KGFL----YKCCGCHKDSVE-EVHSSNNTKTNPKQELVRSGLNFTFRKSTSKLAEPKNLP 243
           K FL     KC     DSV+ E  +S     +P   ++      T R S     E     
Sbjct: 182 KSFLANLGCKCACSDGDSVDVEEKTSVKRSADPNISVI------TMRSSADMNTE----- 241

Query: 244 LKMKIQVEEQ-QARRSIEVFGSSLKAQSVETVSLNHLERKLSMLSWNDIGPRVKESDSNM 303
             +KIQ +E+   R+S+EVFGS +  +   +V    +++KL +  W     R +E D+  
Sbjct: 242 -LIKIQKQEELSQRKSLEVFGSPVAIEKKSSV----VQKKLPLPPWKS---RTEEDDTK- 301

Query: 304 SVSATDDSILIDNGDAESDASSDLFEIESLMGNPNPFLVRQGSDCTDCVTPTTCYAPSEA 363
                            SD+SSDLFEIE L GNP PFL RQGSD        TCYAPSE 
Sbjct: 302 --------------SEGSDSSSDLFEIEGLTGNPKPFLTRQGSD----PASPTCYAPSEV 361

Query: 364 SIEWSVVTASATDYDERRLSTTSPT-------------AAAEEIQRRRSN-------LLM 375
           S+EWS+VTASA D+       TSP               A    QRR+S+        LM
Sbjct: 362 SVEWSIVTASAADFSVMSECATSPVRRNRPTQIPRIPITAKSAPQRRKSSSSSGGNGFLM 383

BLAST of MC09g1678 vs. ExPASy Swiss-Prot
Match: Q9M9T4 (Protein PHYTOCHROME KINASE SUBSTRATE 2 OS=Arabidopsis thaliana OX=3702 GN=PKS2 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 4.0e-27
Identity = 154/478 (32.22%), Postives = 220/478 (46.03%), Query Frame = 0

Query: 4   VSLPPPVCDTNLSQRLSFENNNNNNNHLREASFS----SYLNHAEKEFVRK--LAEAARD 63
           V+L       N+S      NNNN+NN     S S    SYL   E    +K  ++    D
Sbjct: 2   VTLTSSSSTPNVSFDFMMNNNNNSNNLYGPFSSSSTSFSYLTSKEDALTQKNLMSGITND 61

Query: 64  -----RPQEEDGDIEVFGAEKYFNGGMDNP-NPLKLRPRRQQFKKAEQMNVHQKKPKTKI 123
                +   ED +I VFGAEKYFNG MD+  +P  + P        E++ V  K  ++  
Sbjct: 62  VLGINKKASEDLEISVFGAEKYFNGDMDSDHSPRLVSPLPDPEVPIERIFVGPK--QSSK 121

Query: 124 SRSGTPSIRSESSWNSQNTLLQRRNHSGPGESKKKNKSSLKPKGFLYKCCGCHKDSVEEV 183
           + S TPS+RSESSWNSQ+ LLQ +        KKKN         + K   C+    E+ 
Sbjct: 122 NSSETPSLRSESSWNSQSLLLQSKY-----VEKKKN---------IKKNSSCNSYFQEKD 181

Query: 184 HSSNNTKTNPKQELVRSGLNFT------------------FRKSTSKLAEPKNLPLKMKI 243
            SSN+  +N K  L   G                       +K  ++L+   +L  +MKI
Sbjct: 182 MSSNHKVSNKKSFLATLGCRCVCSNWSSVDVVDDKRRSSGLKKIKTQLSFSGDLSSEMKI 241

Query: 244 QVEEQQA----RRSIEVFGSSLKAQSVETVSLNHLERKLSMLSWNDIGPRVKESDSNMSV 303
             ++Q+A    R+S+E+FGS L  + +       +++K     W +     K+ +   SV
Sbjct: 242 HQQQQEAMLEQRKSLEIFGSPLIEKRI-------IQKK---FPW-EYSSSAKKEEHGFSV 301

Query: 304 SATDDSILIDNGDAESDASSDLFEIESLMGNPNPFLVRQGSDCTDCVTPTTCYAPSEASI 363
              ++    ++G   SD S+DLFEIESL G  NPFL RQGS   D       YAPSE SI
Sbjct: 302 KYEEE----EDGSV-SDVSTDLFEIESLTGKANPFLARQGSSDPD---SPDGYAPSEVSI 361

Query: 364 EWSVVTASATDYDERRLSTTSP---------------TAAAEEI--QRRRSN---LLMGC 420
           +WSVVTAS  D+       TSP                 +  EI  QRR+S+   LLMGC
Sbjct: 362 QWSVVTASVADFSVMSECATSPVKKNRSFQIPRIPIMAKSNREIAPQRRKSSSSGLLMGC 421

BLAST of MC09g1678 vs. ExPASy Swiss-Prot
Match: Q8GXS8 (Protein PHYTOCHROME KINASE SUBSTRATE 3 OS=Arabidopsis thaliana OX=3702 GN=PKS3 PE=2 SV=2)

HSP 1 Score: 75.9 bits (185), Expect = 1.3e-12
Identity = 108/361 (29.92%), Postives = 165/361 (45.71%), Query Frame = 0

Query: 50  KLAEAARDRPQEEDGDIEVFGAEKYFNGGMD-----------------------NPNPLK 109
           K++E A  +  + D +I VFGAEKYF+  +D                       NPNP  
Sbjct: 33  KISEKANIK-ADNDAEIGVFGAEKYFSMKLDHVDSTADITKQHEKENTHDHPNANPNP-- 92

Query: 110 LRPRRQQFKKAEQMNVHQKKPKTKISRSGTPSIRSESSWNSQNTLLQRRNHSGPGESKKK 169
             P        +       + KT  SR GTPS+RSESS NSQ T L R N+    + +K 
Sbjct: 93  -NPNPHPHPHPQLTKTTSSRSKT--SRHGTPSVRSESSCNSQ-TFLMRINNHNENKQRKM 152

Query: 170 NKSSLKPKGFLYKCCG-CHKDSVEEVHSSNNTKTNPKQELVRSGLNFTFRKSTSKLAEPK 229
           N +S+   GF  +C G C    V+ VH+        +    R  + +  RK   K   P+
Sbjct: 153 NDTSISFGGF--RCYGPC--SGVKTVHTDPKNSCKSRNS-DRDFVAYDARKHNDK---PR 212

Query: 230 NLPLKMKIQVEEQQARRSIEVFGSSLKAQSVETVSLNHLERKLSMLSWNDIGPRVKESDS 289
              L  + +  +  A+  I     SL  Q  + +++N LERKLS+L+W+ I  ++   ++
Sbjct: 213 ---LHFEAKKADFHAQEKI-----SLPIQRSD-IAMN-LERKLSLLTWDAIPNQLSTKNN 272

Query: 290 NMSVSATDDSILIDNGDAE--SDASSDLFEIESLMGNPNPFLVRQGSDCTDCVTPTTCYA 349
           N + +  + S+  +  + E  S ASSDLFEIE++                     ++ Y 
Sbjct: 273 NHNNNGNNSSMSSNTQEEETASVASSDLFEIENI--------------------TSSVYE 332

Query: 350 PSEASIEWSVVTAS------ATDYD-ERRLSTTSPTAAAEEI---QRRRSNLLMGCRSKK 375
           PSEASI WSVVT S       +D+D  +R++   P    + +   + R +  L GC+S K
Sbjct: 333 PSEASIGWSVVTGSMADQSVISDFDMMKRVTRNGPVVKTKPVIGEKVRSAGFLSGCKSHK 348

BLAST of MC09g1678 vs. ExPASy Swiss-Prot
Match: Q9FYE2 (Protein PHYTOCHROME KINASE SUBSTRATE 4 OS=Arabidopsis thaliana OX=3702 GN=PKS4 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 4.3e-05
Identity = 93/370 (25.14%), Postives = 150/370 (40.54%), Query Frame = 0

Query: 26  NNNNHLREASFSSYLNHAEKEFVRKLAEAARDRPQEEDGDIEVFGAEKYFNGGMDNPNPL 85
           N +N + + SFSSYL     E         + + + ED ++ +F A  YF+    N +  
Sbjct: 25  NRSNDI-DVSFSSYLKPDNNE---------QQQKENEDTELSIFEARSYFSENGSNDSRC 84

Query: 86  KLR----PRRQQFKKAEQMNVHQKKPKTKISRSGTPSIRSESSWNSQNTLLQRRNHSGPG 145
           + R    PR      A       K     + ++ +    SE+SWNSQ  LL  +N  G  
Sbjct: 85  QTRNLSGPRFSSVASA-------KVSSFTVGQTAS----SEASWNSQTGLLSNKNRQGSD 144

Query: 146 ESKKKNKSSLKPKGFLYKCCGCHKDSVEEVHSSNNTKTNPKQELVRSGLNFTFRKSTSKL 205
              +++        F  + C C      +V  S      P+  + ++G +          
Sbjct: 145 RDGRRSSKKGPRWFFRRRACPCSSSKSVQVQES-----KPRIAVPKTGSDRIVSNRIVHS 204

Query: 206 AEPKNLPLKMKIQVEEQQARRSIEVFGSSLKAQSVETVSLNHLERKLSMLSWNDIGP--- 265
            +  + P  +++ +      RSI+   +      V   S   L  + S LS N   P   
Sbjct: 205 HQTISSPEPIRLTIPSNTVTRSIDYTANKEARAPVSNFSFPTL-NETSQLSENPKNPVLN 264

Query: 266 -----RVKES--DSNMSVSATDDSILIDNGDAESDASSDLFEIESL----MGNPNPFLVR 325
                R++ +       ++ T    +I + +A SDASSDLFEIES        P    VR
Sbjct: 265 HIKPVRIEPALLPIKPVLNPTSPKGVIIDEEATSDASSDLFEIESFSTQTAARPWAPPVR 324

Query: 326 QGSDCTDCVTPTTCYAPSEASIEWSVVT---ASATDYDERRLSTTSPTAAAEEIQRRRSN 375
              D  +       Y PSEAS+ WSV+T   ASA   +  R++ +S + A     ++R+ 
Sbjct: 325 ---DSMEETVSEYGYEPSEASVTWSVMTAEPASAVAANFSRIALSSSSTAFSGYDKKRTG 363

BLAST of MC09g1678 vs. NCBI nr
Match: XP_022151078.1 (protein PHYTOCHROME KINASE SUBSTRATE 1-like [Momordica charantia])

HSP 1 Score: 827 bits (2137), Expect = 1.62e-300
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0

Query: 1   MGRVSLPPPVCDTNLSQRLSFENNNNNNNHLREASFSSYLNHAEKEFVRKLAEAARDRPQ 60
           MGRVSLPPPVCDTNLSQRLSFENNNNNNNHLREASFSSYLNHAEKEFVRKLAEAARDRPQ
Sbjct: 94  MGRVSLPPPVCDTNLSQRLSFENNNNNNNHLREASFSSYLNHAEKEFVRKLAEAARDRPQ 153

Query: 61  EEDGDIEVFGAEKYFNGGMDNPNPLKLRPRRQQFKKAEQMNVHQKKPKTKISRSGTPSIR 120
           EEDGDIEVFGAEKYFNGGMDNPNPLKLRPRRQQFKKAEQMNVHQKKPKTKISRSGTPSIR
Sbjct: 154 EEDGDIEVFGAEKYFNGGMDNPNPLKLRPRRQQFKKAEQMNVHQKKPKTKISRSGTPSIR 213

Query: 121 SESSWNSQNTLLQRRNHSGPGESKKKNKSSLKPKGFLYKCCGCHKDSVEEVHSSNNTKTN 180
           SESSWNSQNTLLQRRNHSGPGESKKKNKSSLKPKGFLYKCCGCHKDSVEEVHSSNNTKTN
Sbjct: 214 SESSWNSQNTLLQRRNHSGPGESKKKNKSSLKPKGFLYKCCGCHKDSVEEVHSSNNTKTN 273

Query: 181 PKQELVRSGLNFTFRKSTSKLAEPKNLPLKMKIQVEEQQARRSIEVFGSSLKAQSVETVS 240
           PKQELVRSGLNFTFRKSTSKLAEPKNLPLKMKIQVEEQQARRSIEVFGSSLKAQSVETVS
Sbjct: 274 PKQELVRSGLNFTFRKSTSKLAEPKNLPLKMKIQVEEQQARRSIEVFGSSLKAQSVETVS 333

Query: 241 LNHLERKLSMLSWNDIGPRVKESDSNMSVSATDDSILIDNGDAESDASSDLFEIESLMGN 300
           LNHLERKLSMLSWNDIGPRVKESDSNMSVSATDDSILIDNGDAESDASSDLFEIESLMGN
Sbjct: 334 LNHLERKLSMLSWNDIGPRVKESDSNMSVSATDDSILIDNGDAESDASSDLFEIESLMGN 393

Query: 301 PNPFLVRQGSDCTDCVTPTTCYAPSEASIEWSVVTASATDYDERRLSTTSPTAAAEEIQR 360
           PNPFLVRQGSDCTDCVTPTTCYAPSEASIEWSVVTASATDYDERRLSTTSPTAAAEEIQR
Sbjct: 394 PNPFLVRQGSDCTDCVTPTTCYAPSEASIEWSVVTASATDYDERRLSTTSPTAAAEEIQR 453

Query: 361 RRSNLLMGCRSKKAAGDAYGSEAEVCRRAEAFGRMTRFEAETKVMGFVGTNSISRRYSPQ 420
           RRSNLLMGCRSKKAAGDAYGSEAEVCRRAEAFGRMTRFEAETKVMGFVGTNSISRRYSPQ
Sbjct: 454 RRSNLLMGCRSKKAAGDAYGSEAEVCRRAEAFGRMTRFEAETKVMGFVGTNSISRRYSPQ 513

Query: 421 MSNILY 426
           MSNILY
Sbjct: 514 MSNILY 519

BLAST of MC09g1678 vs. NCBI nr
Match: XP_038893120.1 (protein PHYTOCHROME KINASE SUBSTRATE 1-like [Benincasa hispida])

HSP 1 Score: 478 bits (1231), Expect = 8.04e-164
Identity = 290/471 (61.57%), Postives = 340/471 (72.19%), Query Frame = 0

Query: 1   MGRVSLPPPVCDTNLSQRLSFENNNNNNN-HLREASFSSYLNHAEKEFVRKLAEAARDR- 60
           MGRVSLPPPVCDTNLS RLSFENNNNNNN HLREASFSSYLNHAEKEFVRKLAE+ RD  
Sbjct: 1   MGRVSLPPPVCDTNLSHRLSFENNNNNNNNHLREASFSSYLNHAEKEFVRKLAESTRDHR 60

Query: 61  -----PQEEDGDIEVFGAEKYFNGGM-DNPNPLKLRPRRQQFKKAEQMNVHQKKPKTKIS 120
                 QEEDGDIEVFGAEKYFNGG+ + P PLK RP++QQ     +M    KKPKT   
Sbjct: 61  SNSITTQEEDGDIEVFGAEKYFNGGIFETPKPLKFRPKKQQ-----RMIHKHKKPKT--- 120

Query: 121 RSGTPSIRSESSWNSQNTLLQR---RNHSGPGESKKKNKSSLKPKGFLYKCCGCHKDSVE 180
              T S+RSESSWNSQN LLQ      +S   ++ KK KSS   KGFLYKC  C ++SVE
Sbjct: 121 --STSSVRSESSWNSQNALLQSSTVNTNSDSSKATKKKKSSQLAKGFLYKCYCCDQNSVE 180

Query: 181 EVHSSNNTKTNPKQEL-----VRSGLNFTFRKSTSKLAEPKNLPLKMKIQVEEQQARRSI 240
            V++   +K+  K +L      ++G+NFTF+ S      P    +KM+IQVEEQQ R+SI
Sbjct: 181 SVNNQTTSKSKSKAKLKQRQPTKAGINFTFQSSI-----PAETAMKMQIQVEEQQGRKSI 240

Query: 241 EVFGSSL--KAQSVETVSLNHLERKLSMLSWNDIGPRVKESDSNMSVSATDDSILIDNGD 300
           EVFGSSL  K  +V+T++LNHLE++LSM++WNDI PRVK+S SN ++S T+DS LI N D
Sbjct: 241 EVFGSSLMGKPPNVDTLNLNHLEKRLSMMTWNDIVPRVKDS-SNSNISTTNDSNLIYNDD 300

Query: 301 AESDASSDLFEIESLMGNPNPFLVRQGSDCTDCVTPTTCYAPSEASIEWSVVTASA---- 360
           AESDASSDLFEI SL GNPNPFL RQGSD TDCVTPTTCYAPSEASIEWSVVTASA    
Sbjct: 301 AESDASSDLFEIASLTGNPNPFLTRQGSDSTDCVTPTTCYAPSEASIEWSVVTASAADFS 360

Query: 361 --TDYDERRLSTTSP-------TAAA-----------EEIQRRRSNLLMGCRSKKA---A 420
             +DYDERRLST+SP       TAA            +EIQRRRSNLLMGC+++KA   A
Sbjct: 361 VVSDYDERRLSTSSPVRNMTAVTAATYMVKSSYGNDVKEIQRRRSNLLMGCKNQKAVRVA 420

Query: 421 GDAYGSEAEVCRRAEAFGRMTRFEAETKVMGFVGTNSISRRYSPQMSNILY 426
           GD YGS AEV RR+E+FGR+TR E +      + TNS+ R YSP++SNILY
Sbjct: 421 GDKYGSAAEVHRRSESFGRLTRLETQR-----LATNSLPRHYSPKISNILY 450

BLAST of MC09g1678 vs. NCBI nr
Match: XP_004135459.1 (protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis sativus])

HSP 1 Score: 437 bits (1125), Expect = 8.15e-148
Identity = 274/467 (58.67%), Postives = 321/467 (68.74%), Query Frame = 0

Query: 1   MGRVSLPPPVCDTNLSQRLSFENNNNNNN-HLREASFSSYLNHAEKEFVRKLAEAARD-- 60
           MGRVSLPPPVCDTNLSQRLSFENNNNNNN HLREASFSSYLNHAEKEFVRKLAE+ RD  
Sbjct: 1   MGRVSLPPPVCDTNLSQRLSFENNNNNNNYHLREASFSSYLNHAEKEFVRKLAESTRDHH 60

Query: 61  -----RPQEEDGDIEVFGAEKYFNGGM--DNPNPLKLRPRRQQFKKAEQMNV-HQKKPKT 120
                + QE+DGDIEVFGAEKYFNG +    P PLK RP++QQ K    MN+ H   PKT
Sbjct: 61  RSNSTKTQEDDGDIEVFGAEKYFNGAIFETTPKPLKFRPKKQQQK---MMNILHTNHPKT 120

Query: 121 KISRSGTPSIRSESSWNSQNTLLQRR-----NHSGPGESKKKNKSSLKPKGFLYKCCGCH 180
                  PS+RSESSWNSQN LLQ       N +    +KKK  SS   K FLY C    
Sbjct: 121 -------PSLRSESSWNSQNPLLQGSTVVGTNSNSSNPTKKKKNSSQLAKDFLYNCYCWD 180

Query: 181 KDSVEEVHSSNNTKTNPK------QELVRSGLNFTFRKSTSKLAEPKNLPLKMKIQVEEQ 240
             SVE ++ +   K+ PK      Q+  ++G+NFTF+ S      P     KM+IQVEEQ
Sbjct: 181 NKSVESMNHNAKGKSKPKPKPKQKQQQTKTGINFTFQSSI-----PAEAAKKMQIQVEEQ 240

Query: 241 QARRSIEVFGSSLKAQ---SVETVSLNHLERKLSMLSWNDIGPRVKESDSNMSVSATDDS 300
           Q R SIEVFGSSL  +   +++ ++LN+ E+KLS ++WNDI PRVK+S SN ++S TD+S
Sbjct: 241 QGRESIEVFGSSLMGKPSNNLDALNLNNFEKKLSTMTWNDIVPRVKDS-SNSNISPTDNS 300

Query: 301 ILIDNGDAESDASSDLFEIESLMGNPNPFLVRQGSDCTDC-VTPTTCYAPSEASIEWSVV 360
            LI N D ESD+SSDLFEIES+  NPN FL RQGSD TDC VTPTTCYAPSEASIEWSVV
Sbjct: 301 NLIYNDDVESDSSSDLFEIESITKNPNSFLTRQGSDSTDCCVTPTTCYAPSEASIEWSVV 360

Query: 361 TASA------TDYDERRLSTTSPT------AAAEEIQRRRSNLLMGCRSKKA---AGDAY 420
           T SA      +DYDERRLS  SP       AAA  IQRRRSNLLMGC+++KA   AGD Y
Sbjct: 361 TTSAADFSVVSDYDERRLSINSPVRNVTPAAAASYIQRRRSNLLMGCKNQKAVMVAGDKY 420

Query: 421 GSEAEVCRRAEAFGRMTRFEAETKVMGFVGTNSISRRYSPQMSNILY 426
            S  EV RR+E+FGRMTR E +      + TNS+ R YSPQ+S+ILY
Sbjct: 421 SSATEVHRRSESFGRMTRLETQR-----LATNSLPRLYSPQISSILY 446

BLAST of MC09g1678 vs. NCBI nr
Match: XP_008446342.1 (PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucumis melo] >KAA0034410.1 protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 437 bits (1123), Expect = 1.75e-147
Identity = 276/469 (58.85%), Postives = 323/469 (68.87%), Query Frame = 0

Query: 1   MGRVSLPPPVCDTNLSQRLSFENNNNNNN-HLREASFSSYLNHAEKEFVRKLAEAARD-- 60
           MGRVSLPPPVCDTNLS RLSFENNNNNNN HLREASFSSYLNHAEKEFVRKLAE+ RD  
Sbjct: 1   MGRVSLPPPVCDTNLSHRLSFENNNNNNNYHLREASFSSYLNHAEKEFVRKLAESTRDHH 60

Query: 61  -----RPQEEDGDIEVFGAEKYFNGGM--DNPNPLKLRPRRQQFKKAEQMNV-HQKKPKT 120
                + QE+DGDIEVFGAEKYFNG +    P PLK RP+RQQ K    MN+ H   PKT
Sbjct: 61  RSNSIKTQEDDGDIEVFGAEKYFNGAIFETTPKPLKFRPKRQQQK---MMNILHTNHPKT 120

Query: 121 KISRSGTPSIRSESSWNSQNTLLQ-----RRNHSGPGESKKKNK----SSLKPKGFLYKC 180
                  PS+RSESSWNSQN LLQ       N +    +KKKNK    SS   KGFLY C
Sbjct: 121 -------PSLRSESSWNSQNPLLQSSTIVNTNSNASNPTKKKNKKKKNSSQLAKGFLYNC 180

Query: 181 CGCHKDSVEEVHSSNNTKTNPK----QELVRSGLNFTFRKSTSKLAEPKNLPLKMKIQVE 240
                 SVE ++ +   K+ PK    Q+  ++G+NFTF+ S      P     KM+IQVE
Sbjct: 181 SCWDNKSVESINHNAKLKSKPKPKQKQQQTKTGINFTFQSSI-----PAEAAKKMQIQVE 240

Query: 241 EQQARRSIEVFGSSLKAQ---SVETVSLNHLERKLSMLSWNDIGPRVKESDSNMSVSATD 300
           EQQ R SIEVFGSSL  +   +++ ++LN+LE+KLS ++WNDI PRVK+S SN ++  TD
Sbjct: 241 EQQGRESIEVFGSSLMGKPSNNLDALNLNNLEKKLSTMTWNDIVPRVKDS-SNSNIPTTD 300

Query: 301 DSILIDNGDAESDASSDLFEIESLMGNPNPFLVRQGSDCTD-CVTPTTCYAPSEASIEWS 360
           +  LI N D ESDASSDLFEIESL  NP+ FL RQGSD TD CVTPTTCYAPSEASIEWS
Sbjct: 301 NPNLIFNDDMESDASSDLFEIESLTKNPDSFLTRQGSDSTDYCVTPTTCYAPSEASIEWS 360

Query: 361 VVTASA------TDYDERRLSTTSPT------AAAEEIQRRRSNLLMGCRSKKA---AGD 420
           +VTASA      +DYDE RLS +SP       AAA  I+RRRSNLLMGC+++KA   AGD
Sbjct: 361 IVTASAADFSVVSDYDEHRLSISSPVRNVTSAAAATYIRRRRSNLLMGCKNQKAVMVAGD 420

Query: 421 AYGSEAEVCRRAEAFGRMTRFEAETKVMGFVGTNSISRRYSPQMSNILY 426
            YGS AEV  R+E+FGRMTR E +        TNS+ R YSPQ+S+ILY
Sbjct: 421 KYGSAAEVHGRSESFGRMTRLETQRHA-----TNSLPRLYSPQISSILY 448

BLAST of MC09g1678 vs. NCBI nr
Match: KAE8648742.1 (hypothetical protein Csa_008509 [Cucumis sativus])

HSP 1 Score: 417 bits (1072), Expect = 5.39e-140
Identity = 261/439 (59.45%), Postives = 304/439 (69.25%), Query Frame = 0

Query: 1   MGRVSLPPPVCDTNLSQRLSFENNNNNNN-HLREASFSSYLNHAEKEFVRKLAEAARD-- 60
           MGRVSLPPPVCDTNLSQRLSFENNNNNNN HLREASFSSYLNHAEKEFVRKLAE+ RD  
Sbjct: 1   MGRVSLPPPVCDTNLSQRLSFENNNNNNNYHLREASFSSYLNHAEKEFVRKLAESTRDHH 60

Query: 61  -----RPQEEDGDIEVFGAEKYFNGGM--DNPNPLKLRPRRQQFKKAEQMNV-HQKKPKT 120
                + QE+DGDIEVFGAEKYFNG +    P PLK RP++QQ K    MN+ H   PKT
Sbjct: 61  RSNSTKTQEDDGDIEVFGAEKYFNGAIFETTPKPLKFRPKKQQQK---MMNILHTNHPKT 120

Query: 121 KISRSGTPSIRSESSWNSQNTLLQRR-----NHSGPGESKKKNKSSLKPKGFLYKCCGCH 180
                  PS+RSESSWNSQN LLQ       N +    +KKK  SS   K FLY C    
Sbjct: 121 -------PSLRSESSWNSQNPLLQGSTVVGTNSNSSNPTKKKKNSSQLAKDFLYNCYCWD 180

Query: 181 KDSVEEVHSSNNTKTNPK------QELVRSGLNFTFRKSTSKLAEPKNLPLKMKIQVEEQ 240
             SVE ++ +   K+ PK      Q+  ++G+NFTF+ S      P     KM+IQVEEQ
Sbjct: 181 NKSVESMNHNAKGKSKPKPKPKQKQQQTKTGINFTFQSSI-----PAEAAKKMQIQVEEQ 240

Query: 241 QARRSIEVFGSSLKAQ---SVETVSLNHLERKLSMLSWNDIGPRVKESDSNMSVSATDDS 300
           Q R SIEVFGSSL  +   +++ ++LN+ E+KLS ++WNDI PRVK+S SN ++S TD+S
Sbjct: 241 QGRESIEVFGSSLMGKPSNNLDALNLNNFEKKLSTMTWNDIVPRVKDS-SNSNISPTDNS 300

Query: 301 ILIDNGDAESDASSDLFEIESLMGNPNPFLVRQGSDCTDC-VTPTTCYAPSEASIEWSVV 360
            LI N D ESD+SSDLFEIES+  NPN FL RQGSD TDC VTPTTCYAPSEASIEWSVV
Sbjct: 301 NLIYNDDVESDSSSDLFEIESITKNPNSFLTRQGSDSTDCCVTPTTCYAPSEASIEWSVV 360

Query: 361 TASA------TDYDERRLSTTSPT------AAAEEIQRRRSNLLMGCRSKKA---AGDAY 398
           T SA      +DYDERRLS  SP       AAA  IQRRRSNLLMGC+++KA   AGD Y
Sbjct: 361 TTSAADFSVVSDYDERRLSINSPVRNVTPAAAASYIQRRRSNLLMGCKNQKAVMVAGDKY 420

BLAST of MC09g1678 vs. ExPASy TrEMBL
Match: A0A6J1DB72 (protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Momordica charantia OX=3673 GN=LOC111019100 PE=4 SV=1)

HSP 1 Score: 827 bits (2137), Expect = 7.82e-301
Identity = 426/426 (100.00%), Postives = 426/426 (100.00%), Query Frame = 0

Query: 1   MGRVSLPPPVCDTNLSQRLSFENNNNNNNHLREASFSSYLNHAEKEFVRKLAEAARDRPQ 60
           MGRVSLPPPVCDTNLSQRLSFENNNNNNNHLREASFSSYLNHAEKEFVRKLAEAARDRPQ
Sbjct: 94  MGRVSLPPPVCDTNLSQRLSFENNNNNNNHLREASFSSYLNHAEKEFVRKLAEAARDRPQ 153

Query: 61  EEDGDIEVFGAEKYFNGGMDNPNPLKLRPRRQQFKKAEQMNVHQKKPKTKISRSGTPSIR 120
           EEDGDIEVFGAEKYFNGGMDNPNPLKLRPRRQQFKKAEQMNVHQKKPKTKISRSGTPSIR
Sbjct: 154 EEDGDIEVFGAEKYFNGGMDNPNPLKLRPRRQQFKKAEQMNVHQKKPKTKISRSGTPSIR 213

Query: 121 SESSWNSQNTLLQRRNHSGPGESKKKNKSSLKPKGFLYKCCGCHKDSVEEVHSSNNTKTN 180
           SESSWNSQNTLLQRRNHSGPGESKKKNKSSLKPKGFLYKCCGCHKDSVEEVHSSNNTKTN
Sbjct: 214 SESSWNSQNTLLQRRNHSGPGESKKKNKSSLKPKGFLYKCCGCHKDSVEEVHSSNNTKTN 273

Query: 181 PKQELVRSGLNFTFRKSTSKLAEPKNLPLKMKIQVEEQQARRSIEVFGSSLKAQSVETVS 240
           PKQELVRSGLNFTFRKSTSKLAEPKNLPLKMKIQVEEQQARRSIEVFGSSLKAQSVETVS
Sbjct: 274 PKQELVRSGLNFTFRKSTSKLAEPKNLPLKMKIQVEEQQARRSIEVFGSSLKAQSVETVS 333

Query: 241 LNHLERKLSMLSWNDIGPRVKESDSNMSVSATDDSILIDNGDAESDASSDLFEIESLMGN 300
           LNHLERKLSMLSWNDIGPRVKESDSNMSVSATDDSILIDNGDAESDASSDLFEIESLMGN
Sbjct: 334 LNHLERKLSMLSWNDIGPRVKESDSNMSVSATDDSILIDNGDAESDASSDLFEIESLMGN 393

Query: 301 PNPFLVRQGSDCTDCVTPTTCYAPSEASIEWSVVTASATDYDERRLSTTSPTAAAEEIQR 360
           PNPFLVRQGSDCTDCVTPTTCYAPSEASIEWSVVTASATDYDERRLSTTSPTAAAEEIQR
Sbjct: 394 PNPFLVRQGSDCTDCVTPTTCYAPSEASIEWSVVTASATDYDERRLSTTSPTAAAEEIQR 453

Query: 361 RRSNLLMGCRSKKAAGDAYGSEAEVCRRAEAFGRMTRFEAETKVMGFVGTNSISRRYSPQ 420
           RRSNLLMGCRSKKAAGDAYGSEAEVCRRAEAFGRMTRFEAETKVMGFVGTNSISRRYSPQ
Sbjct: 454 RRSNLLMGCRSKKAAGDAYGSEAEVCRRAEAFGRMTRFEAETKVMGFVGTNSISRRYSPQ 513

Query: 421 MSNILY 426
           MSNILY
Sbjct: 514 MSNILY 519

BLAST of MC09g1678 vs. ExPASy TrEMBL
Match: A0A0A0KSN7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G605030 PE=4 SV=1)

HSP 1 Score: 437 bits (1125), Expect = 3.95e-148
Identity = 274/467 (58.67%), Postives = 321/467 (68.74%), Query Frame = 0

Query: 1   MGRVSLPPPVCDTNLSQRLSFENNNNNNN-HLREASFSSYLNHAEKEFVRKLAEAARD-- 60
           MGRVSLPPPVCDTNLSQRLSFENNNNNNN HLREASFSSYLNHAEKEFVRKLAE+ RD  
Sbjct: 1   MGRVSLPPPVCDTNLSQRLSFENNNNNNNYHLREASFSSYLNHAEKEFVRKLAESTRDHH 60

Query: 61  -----RPQEEDGDIEVFGAEKYFNGGM--DNPNPLKLRPRRQQFKKAEQMNV-HQKKPKT 120
                + QE+DGDIEVFGAEKYFNG +    P PLK RP++QQ K    MN+ H   PKT
Sbjct: 61  RSNSTKTQEDDGDIEVFGAEKYFNGAIFETTPKPLKFRPKKQQQK---MMNILHTNHPKT 120

Query: 121 KISRSGTPSIRSESSWNSQNTLLQRR-----NHSGPGESKKKNKSSLKPKGFLYKCCGCH 180
                  PS+RSESSWNSQN LLQ       N +    +KKK  SS   K FLY C    
Sbjct: 121 -------PSLRSESSWNSQNPLLQGSTVVGTNSNSSNPTKKKKNSSQLAKDFLYNCYCWD 180

Query: 181 KDSVEEVHSSNNTKTNPK------QELVRSGLNFTFRKSTSKLAEPKNLPLKMKIQVEEQ 240
             SVE ++ +   K+ PK      Q+  ++G+NFTF+ S      P     KM+IQVEEQ
Sbjct: 181 NKSVESMNHNAKGKSKPKPKPKQKQQQTKTGINFTFQSSI-----PAEAAKKMQIQVEEQ 240

Query: 241 QARRSIEVFGSSLKAQ---SVETVSLNHLERKLSMLSWNDIGPRVKESDSNMSVSATDDS 300
           Q R SIEVFGSSL  +   +++ ++LN+ E+KLS ++WNDI PRVK+S SN ++S TD+S
Sbjct: 241 QGRESIEVFGSSLMGKPSNNLDALNLNNFEKKLSTMTWNDIVPRVKDS-SNSNISPTDNS 300

Query: 301 ILIDNGDAESDASSDLFEIESLMGNPNPFLVRQGSDCTDC-VTPTTCYAPSEASIEWSVV 360
            LI N D ESD+SSDLFEIES+  NPN FL RQGSD TDC VTPTTCYAPSEASIEWSVV
Sbjct: 301 NLIYNDDVESDSSSDLFEIESITKNPNSFLTRQGSDSTDCCVTPTTCYAPSEASIEWSVV 360

Query: 361 TASA------TDYDERRLSTTSPT------AAAEEIQRRRSNLLMGCRSKKA---AGDAY 420
           T SA      +DYDERRLS  SP       AAA  IQRRRSNLLMGC+++KA   AGD Y
Sbjct: 361 TTSAADFSVVSDYDERRLSINSPVRNVTPAAAASYIQRRRSNLLMGCKNQKAVMVAGDKY 420

Query: 421 GSEAEVCRRAEAFGRMTRFEAETKVMGFVGTNSISRRYSPQMSNILY 426
            S  EV RR+E+FGRMTR E +      + TNS+ R YSPQ+S+ILY
Sbjct: 421 SSATEVHRRSESFGRMTRLETQR-----LATNSLPRLYSPQISSILY 446

BLAST of MC09g1678 vs. ExPASy TrEMBL
Match: A0A5A7STW5 (Protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G004160 PE=4 SV=1)

HSP 1 Score: 437 bits (1123), Expect = 8.48e-148
Identity = 276/469 (58.85%), Postives = 323/469 (68.87%), Query Frame = 0

Query: 1   MGRVSLPPPVCDTNLSQRLSFENNNNNNN-HLREASFSSYLNHAEKEFVRKLAEAARD-- 60
           MGRVSLPPPVCDTNLS RLSFENNNNNNN HLREASFSSYLNHAEKEFVRKLAE+ RD  
Sbjct: 1   MGRVSLPPPVCDTNLSHRLSFENNNNNNNYHLREASFSSYLNHAEKEFVRKLAESTRDHH 60

Query: 61  -----RPQEEDGDIEVFGAEKYFNGGM--DNPNPLKLRPRRQQFKKAEQMNV-HQKKPKT 120
                + QE+DGDIEVFGAEKYFNG +    P PLK RP+RQQ K    MN+ H   PKT
Sbjct: 61  RSNSIKTQEDDGDIEVFGAEKYFNGAIFETTPKPLKFRPKRQQQK---MMNILHTNHPKT 120

Query: 121 KISRSGTPSIRSESSWNSQNTLLQ-----RRNHSGPGESKKKNK----SSLKPKGFLYKC 180
                  PS+RSESSWNSQN LLQ       N +    +KKKNK    SS   KGFLY C
Sbjct: 121 -------PSLRSESSWNSQNPLLQSSTIVNTNSNASNPTKKKNKKKKNSSQLAKGFLYNC 180

Query: 181 CGCHKDSVEEVHSSNNTKTNPK----QELVRSGLNFTFRKSTSKLAEPKNLPLKMKIQVE 240
                 SVE ++ +   K+ PK    Q+  ++G+NFTF+ S      P     KM+IQVE
Sbjct: 181 SCWDNKSVESINHNAKLKSKPKPKQKQQQTKTGINFTFQSSI-----PAEAAKKMQIQVE 240

Query: 241 EQQARRSIEVFGSSLKAQ---SVETVSLNHLERKLSMLSWNDIGPRVKESDSNMSVSATD 300
           EQQ R SIEVFGSSL  +   +++ ++LN+LE+KLS ++WNDI PRVK+S SN ++  TD
Sbjct: 241 EQQGRESIEVFGSSLMGKPSNNLDALNLNNLEKKLSTMTWNDIVPRVKDS-SNSNIPTTD 300

Query: 301 DSILIDNGDAESDASSDLFEIESLMGNPNPFLVRQGSDCTD-CVTPTTCYAPSEASIEWS 360
           +  LI N D ESDASSDLFEIESL  NP+ FL RQGSD TD CVTPTTCYAPSEASIEWS
Sbjct: 301 NPNLIFNDDMESDASSDLFEIESLTKNPDSFLTRQGSDSTDYCVTPTTCYAPSEASIEWS 360

Query: 361 VVTASA------TDYDERRLSTTSPT------AAAEEIQRRRSNLLMGCRSKKA---AGD 420
           +VTASA      +DYDE RLS +SP       AAA  I+RRRSNLLMGC+++KA   AGD
Sbjct: 361 IVTASAADFSVVSDYDEHRLSISSPVRNVTSAAAATYIRRRRSNLLMGCKNQKAVMVAGD 420

Query: 421 AYGSEAEVCRRAEAFGRMTRFEAETKVMGFVGTNSISRRYSPQMSNILY 426
            YGS AEV  R+E+FGRMTR E +        TNS+ R YSPQ+S+ILY
Sbjct: 421 KYGSAAEVHGRSESFGRMTRLETQRHA-----TNSLPRLYSPQISSILY 448

BLAST of MC09g1678 vs. ExPASy TrEMBL
Match: A0A1S3BFN1 (protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucumis melo OX=3656 GN=LOC103489110 PE=4 SV=1)

HSP 1 Score: 437 bits (1123), Expect = 8.48e-148
Identity = 276/469 (58.85%), Postives = 323/469 (68.87%), Query Frame = 0

Query: 1   MGRVSLPPPVCDTNLSQRLSFENNNNNNN-HLREASFSSYLNHAEKEFVRKLAEAARD-- 60
           MGRVSLPPPVCDTNLS RLSFENNNNNNN HLREASFSSYLNHAEKEFVRKLAE+ RD  
Sbjct: 1   MGRVSLPPPVCDTNLSHRLSFENNNNNNNYHLREASFSSYLNHAEKEFVRKLAESTRDHH 60

Query: 61  -----RPQEEDGDIEVFGAEKYFNGGM--DNPNPLKLRPRRQQFKKAEQMNV-HQKKPKT 120
                + QE+DGDIEVFGAEKYFNG +    P PLK RP+RQQ K    MN+ H   PKT
Sbjct: 61  RSNSIKTQEDDGDIEVFGAEKYFNGAIFETTPKPLKFRPKRQQQK---MMNILHTNHPKT 120

Query: 121 KISRSGTPSIRSESSWNSQNTLLQ-----RRNHSGPGESKKKNK----SSLKPKGFLYKC 180
                  PS+RSESSWNSQN LLQ       N +    +KKKNK    SS   KGFLY C
Sbjct: 121 -------PSLRSESSWNSQNPLLQSSTIVNTNSNASNPTKKKNKKKKNSSQLAKGFLYNC 180

Query: 181 CGCHKDSVEEVHSSNNTKTNPK----QELVRSGLNFTFRKSTSKLAEPKNLPLKMKIQVE 240
                 SVE ++ +   K+ PK    Q+  ++G+NFTF+ S      P     KM+IQVE
Sbjct: 181 SCWDNKSVESINHNAKLKSKPKPKQKQQQTKTGINFTFQSSI-----PAEAAKKMQIQVE 240

Query: 241 EQQARRSIEVFGSSLKAQ---SVETVSLNHLERKLSMLSWNDIGPRVKESDSNMSVSATD 300
           EQQ R SIEVFGSSL  +   +++ ++LN+LE+KLS ++WNDI PRVK+S SN ++  TD
Sbjct: 241 EQQGRESIEVFGSSLMGKPSNNLDALNLNNLEKKLSTMTWNDIVPRVKDS-SNSNIPTTD 300

Query: 301 DSILIDNGDAESDASSDLFEIESLMGNPNPFLVRQGSDCTD-CVTPTTCYAPSEASIEWS 360
           +  LI N D ESDASSDLFEIESL  NP+ FL RQGSD TD CVTPTTCYAPSEASIEWS
Sbjct: 301 NPNLIFNDDMESDASSDLFEIESLTKNPDSFLTRQGSDSTDYCVTPTTCYAPSEASIEWS 360

Query: 361 VVTASA------TDYDERRLSTTSPT------AAAEEIQRRRSNLLMGCRSKKA---AGD 420
           +VTASA      +DYDE RLS +SP       AAA  I+RRRSNLLMGC+++KA   AGD
Sbjct: 361 IVTASAADFSVVSDYDEHRLSISSPVRNVTSAAAATYIRRRRSNLLMGCKNQKAVMVAGD 420

Query: 421 AYGSEAEVCRRAEAFGRMTRFEAETKVMGFVGTNSISRRYSPQMSNILY 426
            YGS AEV  R+E+FGRMTR E +        TNS+ R YSPQ+S+ILY
Sbjct: 421 KYGSAAEVHGRSESFGRMTRLETQRHA-----TNSLPRLYSPQISSILY 448

BLAST of MC09g1678 vs. ExPASy TrEMBL
Match: A0A6J1GYD9 (protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458631 PE=4 SV=1)

HSP 1 Score: 393 bits (1010), Expect = 3.11e-131
Identity = 250/449 (55.68%), Postives = 303/449 (67.48%), Query Frame = 0

Query: 1   MGR-VSLPPPVCDTNLSQRLSFENNNNNNNHLREASFSSYLNHAEKEFVRKLAEAARDRP 60
           MGR VSLPPPVCDTNL              HLREASFSSYLNHAEKEFVRKLAE+ RDR 
Sbjct: 1   MGRRVSLPPPVCDTNLP-------------HLREASFSSYLNHAEKEFVRKLAESTRDRS 60

Query: 61  ------QEEDGDIEVFGAEKYFNGG-MDNPNPLKLRPRRQQFKKAEQMNVHQKKPKTKIS 120
                 +E+DGDIEVFGAEKYFN G  D   PLK  P     KK  +  +H KKP+T  S
Sbjct: 61  NSVTTQEEQDGDIEVFGAEKYFNAGAFDTQKPLKFPP-----KKPHKRILHSKKPRTSTS 120

Query: 121 -----RSGTPSIRSESSWNSQNTLLQRRN---HSGPGESKKKNKSSLKPKGFLYKCCGCH 180
                RSG+PS+RS+SSWNSQ  LLQ       S   ++ KK+KSS   KGFLY+C  C 
Sbjct: 121 STTTTRSGSPSVRSQSSWNSQKALLQSSTVNADSMSSKATKKSKSSQLAKGFLYRCYCCD 180

Query: 181 KDSVEEVHSSNNTKTNPKQ-ELVRSGLNFTFRKSTSKLAEPKNLP--LKMKIQVEEQQAR 240
           K+  +       +K+NPK+ +   +G NFTF  S      PKN    LKM+IQVEEQQ +
Sbjct: 181 KNLTK-------SKSNPKRRQTTNAGSNFTFESSVPVDLTPKNSASALKMQIQVEEQQGQ 240

Query: 241 RSIEVFGSSLKAQSVETVSLNHLERKLSMLSWNDIGPRVKESDSNMSVSATDDSILIDNG 300
           + IEVF SSL   +V+++++NHLE++LSM++WNDI P+VK+S ++ S        LI + 
Sbjct: 241 KPIEVFESSLP--NVDSLNVNHLEKRLSMMTWNDIVPKVKDSSNSNSNKCN----LIYHD 300

Query: 301 DAESDASSDLFEIESLMGNPNPFLVRQGSDCTDCVTPTTCYAPSEASIEWSVVTASATDY 360
           DA SDASSDLFEIE+L GNPN FL  Q SDCTDCVTPTTCYAPSEASIEWSV+TASATD+
Sbjct: 301 DAGSDASSDLFEIETLTGNPNSFLTTQPSDCTDCVTPTTCYAPSEASIEWSVITASATDF 360

Query: 361 -DERRLSTTSPTAAAEEIQRRRSNLLMGCRSKKA---AGDAYGSEAEVCRRAEAFGRMTR 420
            DE RLSTTS    A    RRRSN LMGC+++KA   AGD YGS  E+ RR E+FGR+TR
Sbjct: 361 SDEHRLSTTSHDVKAS--LRRRSNHLMGCKNEKAVRVAGDKYGSPEEIQRRWESFGRLTR 411

Query: 421 FEAETKVMGFVGTNSISRRYSPQMSNILY 426
           FEA+      +  NS+ R+YSP++SNILY
Sbjct: 421 FEAQR-----LAANSVPRQYSPKISNILY 411

BLAST of MC09g1678 vs. TAIR 10
Match: AT2G02950.1 (phytochrome kinase substrate 1 )

HSP 1 Score: 131.3 bits (329), Expect = 1.8e-30
Identity = 148/427 (34.66%), Postives = 195/427 (45.67%), Query Frame = 0

Query: 4   VSLPPPVCDTNLSQRLSFENNNNNNNHLR-EASFSSYLNHAEKEFV--RKLAEAAR---- 63
           V+L P    T    + SF+   NNN+H     S SSYL+  E   V  +KL E ++    
Sbjct: 2   VTLTPSSAST---PKTSFDFMKNNNSHSSLYVSSSSYLSSKEDALVTTKKLMEPSKTLNM 61

Query: 64  --DRPQEEDGD-------------IEVFGAEKYFNGGMDNPNPLKLRPRRQQFKKAEQMN 123
             +  QEE GD             I VFGAEKYFNG MD+     +        + E+  
Sbjct: 62  SINPKQEEFGDEKKMVKKAPEDPEIGVFGAEKYFNGDMDSDQGSSVLSLTN--PEVERTV 121

Query: 124 VHQKKPKTKISRSGTPSIRSESSWNSQNTLLQRR--NHSGPGESKKKN------KSSLKP 183
           V  K+   K   +GTPS+RSESSWNSQ+ LLQ +  N       +KKN      K +   
Sbjct: 122 VDSKQSAKK--STGTPSVRSESSWNSQSVLLQNKLVNSCNSSFKEKKNSNGQIQKVTNNK 181

Query: 184 KGFL----YKCCGCHKDSVE-EVHSSNNTKTNPKQELVRSGLNFTFRKSTSKLAEPKNLP 243
           K FL     KC     DSV+ E  +S     +P   ++      T R S     E     
Sbjct: 182 KSFLANLGCKCACSDGDSVDVEEKTSVKRSADPNISVI------TMRSSADMNTE----- 241

Query: 244 LKMKIQVEEQ-QARRSIEVFGSSLKAQSVETVSLNHLERKLSMLSWNDIGPRVKESDSNM 303
             +KIQ +E+   R+S+EVFGS +  +   +V    +++KL +  W     R +E D+  
Sbjct: 242 -LIKIQKQEELSQRKSLEVFGSPVAIEKKSSV----VQKKLPLPPWKS---RTEEDDTK- 301

Query: 304 SVSATDDSILIDNGDAESDASSDLFEIESLMGNPNPFLVRQGSDCTDCVTPTTCYAPSEA 363
                            SD+SSDLFEIE L GNP PFL RQGSD        TCYAPSE 
Sbjct: 302 --------------SEGSDSSSDLFEIEGLTGNPKPFLTRQGSD----PASPTCYAPSEV 361

Query: 364 SIEWSVVTASATDYDERRLSTTSPT-------------AAAEEIQRRRSN-------LLM 375
           S+EWS+VTASA D+       TSP               A    QRR+S+        LM
Sbjct: 362 SVEWSIVTASAADFSVMSECATSPVRRNRPTQIPRIPITAKSAPQRRKSSSSSGGNGFLM 383

BLAST of MC09g1678 vs. TAIR 10
Match: AT1G14280.1 (phytochrome kinase substrate 2 )

HSP 1 Score: 124.0 bits (310), Expect = 2.9e-28
Identity = 154/478 (32.22%), Postives = 220/478 (46.03%), Query Frame = 0

Query: 4   VSLPPPVCDTNLSQRLSFENNNNNNNHLREASFS----SYLNHAEKEFVRK--LAEAARD 63
           V+L       N+S      NNNN+NN     S S    SYL   E    +K  ++    D
Sbjct: 2   VTLTSSSSTPNVSFDFMMNNNNNSNNLYGPFSSSSTSFSYLTSKEDALTQKNLMSGITND 61

Query: 64  -----RPQEEDGDIEVFGAEKYFNGGMDNP-NPLKLRPRRQQFKKAEQMNVHQKKPKTKI 123
                +   ED +I VFGAEKYFNG MD+  +P  + P        E++ V  K  ++  
Sbjct: 62  VLGINKKASEDLEISVFGAEKYFNGDMDSDHSPRLVSPLPDPEVPIERIFVGPK--QSSK 121

Query: 124 SRSGTPSIRSESSWNSQNTLLQRRNHSGPGESKKKNKSSLKPKGFLYKCCGCHKDSVEEV 183
           + S TPS+RSESSWNSQ+ LLQ +        KKKN         + K   C+    E+ 
Sbjct: 122 NSSETPSLRSESSWNSQSLLLQSKY-----VEKKKN---------IKKNSSCNSYFQEKD 181

Query: 184 HSSNNTKTNPKQELVRSGLNFT------------------FRKSTSKLAEPKNLPLKMKI 243
            SSN+  +N K  L   G                       +K  ++L+   +L  +MKI
Sbjct: 182 MSSNHKVSNKKSFLATLGCRCVCSNWSSVDVVDDKRRSSGLKKIKTQLSFSGDLSSEMKI 241

Query: 244 QVEEQQA----RRSIEVFGSSLKAQSVETVSLNHLERKLSMLSWNDIGPRVKESDSNMSV 303
             ++Q+A    R+S+E+FGS L  + +       +++K     W +     K+ +   SV
Sbjct: 242 HQQQQEAMLEQRKSLEIFGSPLIEKRI-------IQKK---FPW-EYSSSAKKEEHGFSV 301

Query: 304 SATDDSILIDNGDAESDASSDLFEIESLMGNPNPFLVRQGSDCTDCVTPTTCYAPSEASI 363
              ++    ++G   SD S+DLFEIESL G  NPFL RQGS   D       YAPSE SI
Sbjct: 302 KYEEE----EDGSV-SDVSTDLFEIESLTGKANPFLARQGSSDPD---SPDGYAPSEVSI 361

Query: 364 EWSVVTASATDYDERRLSTTSP---------------TAAAEEI--QRRRSN---LLMGC 420
           +WSVVTAS  D+       TSP                 +  EI  QRR+S+   LLMGC
Sbjct: 362 QWSVVTASVADFSVMSECATSPVKKNRSFQIPRIPIMAKSNREIAPQRRKSSSSGLLMGC 421

BLAST of MC09g1678 vs. TAIR 10
Match: AT1G18810.1 (phytochrome kinase substrate-related )

HSP 1 Score: 75.9 bits (185), Expect = 8.9e-14
Identity = 108/361 (29.92%), Postives = 165/361 (45.71%), Query Frame = 0

Query: 50  KLAEAARDRPQEEDGDIEVFGAEKYFNGGMD-----------------------NPNPLK 109
           K++E A  +  + D +I VFGAEKYF+  +D                       NPNP  
Sbjct: 33  KISEKANIK-ADNDAEIGVFGAEKYFSMKLDHVDSTADITKQHEKENTHDHPNANPNP-- 92

Query: 110 LRPRRQQFKKAEQMNVHQKKPKTKISRSGTPSIRSESSWNSQNTLLQRRNHSGPGESKKK 169
             P        +       + KT  SR GTPS+RSESS NSQ T L R N+    + +K 
Sbjct: 93  -NPNPHPHPHPQLTKTTSSRSKT--SRHGTPSVRSESSCNSQ-TFLMRINNHNENKQRKM 152

Query: 170 NKSSLKPKGFLYKCCG-CHKDSVEEVHSSNNTKTNPKQELVRSGLNFTFRKSTSKLAEPK 229
           N +S+   GF  +C G C    V+ VH+        +    R  + +  RK   K   P+
Sbjct: 153 NDTSISFGGF--RCYGPC--SGVKTVHTDPKNSCKSRNS-DRDFVAYDARKHNDK---PR 212

Query: 230 NLPLKMKIQVEEQQARRSIEVFGSSLKAQSVETVSLNHLERKLSMLSWNDIGPRVKESDS 289
              L  + +  +  A+  I     SL  Q  + +++N LERKLS+L+W+ I  ++   ++
Sbjct: 213 ---LHFEAKKADFHAQEKI-----SLPIQRSD-IAMN-LERKLSLLTWDAIPNQLSTKNN 272

Query: 290 NMSVSATDDSILIDNGDAE--SDASSDLFEIESLMGNPNPFLVRQGSDCTDCVTPTTCYA 349
           N + +  + S+  +  + E  S ASSDLFEIE++                     ++ Y 
Sbjct: 273 NHNNNGNNSSMSSNTQEEETASVASSDLFEIENI--------------------TSSVYE 332

Query: 350 PSEASIEWSVVTAS------ATDYD-ERRLSTTSPTAAAEEI---QRRRSNLLMGCRSKK 375
           PSEASI WSVVT S       +D+D  +R++   P    + +   + R +  L GC+S K
Sbjct: 333 PSEASIGWSVVTGSMADQSVISDFDMMKRVTRNGPVVKTKPVIGEKVRSAGFLSGCKSHK 348

BLAST of MC09g1678 vs. TAIR 10
Match: AT5G04190.1 (phytochrome kinase substrate 4 )

HSP 1 Score: 50.8 bits (120), Expect = 3.1e-06
Identity = 93/370 (25.14%), Postives = 150/370 (40.54%), Query Frame = 0

Query: 26  NNNNHLREASFSSYLNHAEKEFVRKLAEAARDRPQEEDGDIEVFGAEKYFNGGMDNPNPL 85
           N +N + + SFSSYL     E         + + + ED ++ +F A  YF+    N +  
Sbjct: 25  NRSNDI-DVSFSSYLKPDNNE---------QQQKENEDTELSIFEARSYFSENGSNDSRC 84

Query: 86  KLR----PRRQQFKKAEQMNVHQKKPKTKISRSGTPSIRSESSWNSQNTLLQRRNHSGPG 145
           + R    PR      A       K     + ++ +    SE+SWNSQ  LL  +N  G  
Sbjct: 85  QTRNLSGPRFSSVASA-------KVSSFTVGQTAS----SEASWNSQTGLLSNKNRQGSD 144

Query: 146 ESKKKNKSSLKPKGFLYKCCGCHKDSVEEVHSSNNTKTNPKQELVRSGLNFTFRKSTSKL 205
              +++        F  + C C      +V  S      P+  + ++G +          
Sbjct: 145 RDGRRSSKKGPRWFFRRRACPCSSSKSVQVQES-----KPRIAVPKTGSDRIVSNRIVHS 204

Query: 206 AEPKNLPLKMKIQVEEQQARRSIEVFGSSLKAQSVETVSLNHLERKLSMLSWNDIGP--- 265
            +  + P  +++ +      RSI+   +      V   S   L  + S LS N   P   
Sbjct: 205 HQTISSPEPIRLTIPSNTVTRSIDYTANKEARAPVSNFSFPTL-NETSQLSENPKNPVLN 264

Query: 266 -----RVKES--DSNMSVSATDDSILIDNGDAESDASSDLFEIESL----MGNPNPFLVR 325
                R++ +       ++ T    +I + +A SDASSDLFEIES        P    VR
Sbjct: 265 HIKPVRIEPALLPIKPVLNPTSPKGVIIDEEATSDASSDLFEIESFSTQTAARPWAPPVR 324

Query: 326 QGSDCTDCVTPTTCYAPSEASIEWSVVT---ASATDYDERRLSTTSPTAAAEEIQRRRSN 375
              D  +       Y PSEAS+ WSV+T   ASA   +  R++ +S + A     ++R+ 
Sbjct: 325 ---DSMEETVSEYGYEPSEASVTWSVMTAEPASAVAANFSRIALSSSSTAFSGYDKKRTG 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SWI12.5e-2934.66Protein PHYTOCHROME KINASE SUBSTRATE 1 OS=Arabidopsis thaliana OX=3702 GN=PKS1 P... [more]
Q9M9T44.0e-2732.22Protein PHYTOCHROME KINASE SUBSTRATE 2 OS=Arabidopsis thaliana OX=3702 GN=PKS2 P... [more]
Q8GXS81.3e-1229.92Protein PHYTOCHROME KINASE SUBSTRATE 3 OS=Arabidopsis thaliana OX=3702 GN=PKS3 P... [more]
Q9FYE24.3e-0525.14Protein PHYTOCHROME KINASE SUBSTRATE 4 OS=Arabidopsis thaliana OX=3702 GN=PKS4 P... [more]
Match NameE-valueIdentityDescription
XP_022151078.11.62e-300100.00protein PHYTOCHROME KINASE SUBSTRATE 1-like [Momordica charantia][more]
XP_038893120.18.04e-16461.57protein PHYTOCHROME KINASE SUBSTRATE 1-like [Benincasa hispida][more]
XP_004135459.18.15e-14858.67protein PHYTOCHROME KINASE SUBSTRATE 1 [Cucumis sativus][more]
XP_008446342.11.75e-14758.85PREDICTED: protein PHYTOCHROME KINASE SUBSTRATE 1-like [Cucumis melo] >KAA003441... [more]
KAE8648742.15.39e-14059.45hypothetical protein Csa_008509 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A6J1DB727.82e-301100.00protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Momordica charantia OX=3673 GN=LO... [more]
A0A0A0KSN73.95e-14858.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G605030 PE=4 SV=1[more]
A0A5A7STW58.48e-14858.85Protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BFN18.48e-14858.85protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucumis melo OX=3656 GN=LOC103489... [more]
A0A6J1GYD93.11e-13155.68protein PHYTOCHROME KINASE SUBSTRATE 1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT2G02950.11.8e-3034.66phytochrome kinase substrate 1 [more]
AT1G14280.12.9e-2832.22phytochrome kinase substrate 2 [more]
AT1G18810.18.9e-1429.92phytochrome kinase substrate-related [more]
AT5G04190.13.1e-0625.14phytochrome kinase substrate 4 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 111..137
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 95..150
NoneNo IPR availablePANTHERPTHR33781:SF4PROTEIN PHYTOCHROME KINASE SUBSTRATE 1coord: 11..407
IPR039615Protein PHYTOCHROME KINASE SUBSTRATEPANTHERPTHR33781PROTEIN PHYTOCHROME KINASE SUBSTRATE 1-RELATEDcoord: 11..407

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g1678.1MC09g1678.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0009638 phototropism
molecular_function GO:0016301 kinase activity