MC09g1625 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g1625
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionAlpha-1,4 glucan phosphorylase
LocationMC09: 21862398 .. 21868181 (-)
RNA-Seq ExpressionMC09g1625
SyntenyMC09g1625
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAACACAGCAATGGCGGCATTGCGATTATCATGGGCCTGCGCTCATTCTAATCCAGATTCAAAGTCCAAATTCGTTTCCAGCTTCACTTCAGAGTCCAGTTTTCGGACCAATTGGACGAGGCTTCTGTTGCTTAGAACCTCGGTCTCAAGTTCTGCAAGAAGGAAATCGTGCATGAGGAACGTAGTCAGCGATCAACAGAAGGAACTAAACGAGCCGGTTAACGGAGAAGGTTAATTGCGCTAAACTGACTTGAGATTTTATCCGACGATAGTCTCCGTAGTGGATATGAATGTATACATGTCTATTAATGGAATCGGTTTCTCAATTTATTGAAAATTTGTCTTTGTAGTTGTAGGCGGCTTCAATAGCTTGCTTCTGGATTCGGCATCCGTTGCCGCGAGTATTAAATACCATTCGGAGTTCACGCCTTCTTTCTCTCCGGAGGGCTTTGAGCTTTCTAAGGCATTCTATGCAACTGCTGAAAGTGTTCGTGATATGCTTATCATCAATTGGAATGCTACGTATGAATACTATGAAAAGATGAATGTGAAGCAAGCCTACTATTTGTCTATGGAGTTTCTACAGGTTATTGTCGACGCTGAATCACCAATTATGTAGTTCATAACTTATTTTAAATTTCCGAGAAGCCTGTTCGTTGTCATTCATGTTAGTGTATGTGAATTTGTGGTTTAATGACAGGGTAGAGCATTGCTAAATGCAATCGGAAATTTAGAGCTCTCTGGAGCTTATGCAGATGCTTTAAGAATGTTGGGATGTGATCTCGAGGATGTAGCAAGGCAGGTTAGCAATATATCGTATAATTGCATCTTGTCTCCTTGTTTCCTGCTTCGGTGAGTGACTTCTAATTTCTGGATTTTGGAGTTCCTTTCCCTGTACAAACACAAGGCTGTAACTTAAATTAGGAGAGGAAGAAAGTTTTCTGCTCATTTCTGGTCTTCAATAAATGTTTATTTCATTCCAACCGACTTCAGGAGCTATCAAAACAATGATACAAAGACTTAAGAGACCTAGGACTGCTACATTCATATGGATGAAAACATTTCAAATTTGAGATAAACTGGCATAAGTGAGTTACATATTTAGATTTTTTTTTTCACTAAAGAGCTCTTGATATCCTTTAAAAGGAGCAAGTTCCTTCAAAGAGAAAACTAGTCTTACAAGTTGCTCTAAGTTGTAGTGTGCTTTAATTTATTTTACATATACACATGGTGTCAACGGATATCTTGAGTGTAGGAGTCGGATGCTGCATTAGGAAATGGAGGATTAGGGCGGCTTGCTTCCTGCTTTCTGGATTCCCTGGCAACCCTAAACTATCCTGCATGGGGCTATGGGCTCAGATACAAGTATGGTTTGTTCAAACAGCTCATTACAAAGGATGGGCAGGAGGAAGTTGCTGAAAATTGGCTAGAGGTACTGTAGTATTTCTTCTATTTTCTAACAAGGTTGGTGGTCTCATTAATCCTGTGTTGCTTGATCAAATTTGTTTCATCAGATGGGTAATCCCTGGGAAATTGCAAGAAATGACGTCTTCTATCCTGTGAAATTCTATGGTGAAGTAATTTCAGGCGCAGATGGAAGTAAGCAATGGGTTGGAGGAGAAGATGTAACTGCTGTTGCCTATGATGTCCCAATACCAGGTTATAAAACTAAAAACACCATAAACCTCCGTCTGTGGTCCACAAAAGTTGCTCCGGAACAATTTAATTTAAGTTATTTCAATGTTGGAGATCATGCCAGTGCATATGCAGCCATAAAGAAAGCTGAGAAGGTATTGAAATTTCATGATAATCCTGCTGAAACTCTATTCGAATTATATCATCTTGTAAGTATTTTGTGTAGCTTCTAATTTCTCAACGCAAAGTTCAGCTATTAAGTAGGCTATGGATTGACCTAAAAGAATAAAATCAGAATGTGAGAACAAATAGCGCATGGAATGCTTCTACTATTTTCCTCACTACTAGCCACATCCAAATTTAATGATGTACTACCGTACTTAATTTGGTAACTTCAAGTGCTTAAATAAAAGAAGAAAATTCTTACACTCCCCTGCAAGATCTGCTACATATTGTACCCCGGAGATGAGTCACTGGAGGGAAAGACTCTTAGATTGAAACAACAATACACATTGTGCTCTGCTTCCCTTCAAGATATTGTTGCACGTTTTGAAAGGAGATCTGGAGAGCCTGTGGACTGGCAAAATTTTCCAGAAAAGGTTGCCGTACAGATGAATGATACGCACCCTACACTTTGCATCCCGGAGCTGATAAGAATATTGATGGATGTGAAAGGGTTGAGCTGGAAAGAAGCTTGGGACATCACTCGAAGGTATTTTTTCATAGTGGCTTACTGGCATATAAATATTTTTGCTGCTACCATGAGTATCAACTACTAAGCCAGCCGTGATATGTTTTAGATTCTTATACAATCCTTGCTTTGCTCAATGAATTTAATTTGCTGTTTTTATTATACTCACTTTTTTCCCGTCATAAAAGCTTATACAGACATAATATCTGAATTATCCATTCTTCTCTGTTTTTTGTTTCTTCCCTTTGCAAGAACTGTTGCGTACACAAATCATACTGTTCTGCCCGAGGCGCTGGAAAAATGGAGTTTCCCTCTCATGCAAGAATTGCTTCCTCGACATGTTGAGATAATTGAAATGATTGATGAGGAGGTAAGCATTGATATTAATGGTTGATCTTTGTTTTGGGACACGGTTAAAAATATAGATTGTTTTGTTGGGCTCCAATATAAGAACAAACGCACCAAATTTTCCGAGACATACCGTCTTCAGGTATATGGAATGACGTATTCTTTATTTCTTTTTATGGATTCAGCTTATCCACTCCATAGTTGCTCAGTACGGTACAAAAGACCTTGAGTTGTTGAAACAAAAACTGAAGCAAATGAGAATTCTAGAAAATTTTGAATTACCAGACTCGGTCATGGAAATGCTAGTAAAGTCAACGGAGGAGTCTGCAATCGATCTCGTTGAGGAAGCTGAAAATGTGGACGACGAATTGTTACCTAGTGAAGAAGGAGATGAATCTGAAGACAAAAATATTGAAAAGAAAACAGAGGTTTCGTTTGGAGTTGATCCAAAGCATCCAAGATTGATTCGAATGGCTAATTTATCTGTTGTTGGTGGATATGCTGTAAATGGTGTTGCAGAAATCCATAGTGAAATTGTTAGAACCGAAGTTTTCAGCGACTTCTATGAGGTAAATCCCACGAATGCCACTGCCTTCTCCCATCTAGCACAACATACTATTCAAAGTATATTATAGAATACAGCTATTCCTGATTAAAGTTTCACTTTATATAAGATTTAATTTGATACCATCACGGACGTGAAACTGAGCTTCTCCTCCATTTGAAGTTTACTAACTTCACATATTACTGTCAGTTGTGGCCTGAGAAATTTCAAAACAAAACAAATGGGGTGACACCGAGAAGATGGATTCGTTTTTGCAATCCGGACCTGAGTACAATCATCACAAAGTGGACTGGAACAGAGCAATGGGTGACAGATACTGAGAAACTGGCAATACTTAGAAAGGTAAAATTTTCAAAAACCGAAACTTTCAAGTCCCTACTTCAGTGAAGATTTATCCAACGGATAAACTTGACTTCCATTTGCGATATAACTGCATGCTTGCAGTACCATTAATTATCATAAACGTATCCGCCTGTTCAATTCCAGGAGGAGTAAGTCTCCATTTCCCAGTATCAAATATTTTCTGGAAACTATTCCGTAGCTTGAGCTAATTAGGTTTGATATTAATGTGGATGACGATTGTATATGCAGTTTGCTGATAACGAGGATCTCCAGTCCATGTGGAAGGAAGCTAAAAGAATAAACAAATTGAAAGTTGTCTCTTTCCTTAAAGAAAAAACTGGCTACTTAGTCAGCCCTGATGCTATGTTTGACGTTCAGGTTATTTTCCTGCCCTTGGACAGTTTACTTTGTGGACAAAGACACCAACAATTTTGACTTTCTGCAGAAAACTTGTGGGGTCGAAATTTTTATTCTTTTCTTTGGTATAACAACCGAAGTGATAAGGACCGCTATGGCTCGTTTTCTTCAAATATGTTGCCCATATAATTATCATTGTTAATATGCTTCCAAGATTAATTGAAGAATATGTTAATTCAGGTAAAACGCATCCACGAATACAAGCGGCAGTTGTTGAACATCATGGGGATCGTCTATCGTTATAAACAAATGAAAGAAATGAATATCGAGGAAGGAAAAGCCAAGTTTGTTCCTCGAGTCTGTATATTTGGTGGGAAAGCATTTGCTACGTATGTCCAAGCAAAAAGAATTGTAAAATTCATCACTGATGTTGGAGCTACCGTCAATAACGATCCTGATATAGGAGACCTTTTGAAGGTATATTGTGGCAGACAAACATATCACATATCGTTCTTTTGCCCCGGTAGTCTTTGCAACTATAGAAACCAATTTTAGCTTTAAACTTGTTTTTGTTATAGGTAGTCTTTGTTCCAGATTATAACGTGAGTGTAGCAGAAGTGCTCATTCCTGGCAGTGATCTATCCCAGCATATTAGGTAGTAAAGTTTAACAAGTTTGGATTTCCTTGCTGACCTTCATGTTCAATTTCATCCCACCATACATCAAACTACTGAAAGTAAATAAACATTCATTTCTTAAATCAAGGCTTATTATATTTTGAAGTCATCACAGTTGTTTTGGAAGGTTAACGTTCATTCTTACTTTTCTGGTTTTGGTATTGACTTCTCTGTTTTTGCGTTGTCTTGTAATTTGATTTCGAAAAGCACGGCTGGAATGGAGGCCAGTGGAACCAGCAACATGAAGTTTGCGATGAATGGCTGCATACTAATTGGAACATTAGATGGAGCAAATGTGGAGATAAGAGAAGAGGTTGGAGAAGATAACTTTTTCCTTTTTGGTGCAAGGGCGCATGAAATTGCTGGCCTAAGAAAAGAAAGATCTCAAGGCAAGGTAAGCTTAGAAACTCTTTGCTTTTTACCAAGCGGGACCGACGTTAATTTTTTTAAAAGAAAAAGCTTAATGAATAAAGGGGAAAATATACTTTGATCCCTTGATGGTTGCATAACTCATAATTCTGATTTTATGCATAGTTTGTTCCGGACCCGCGGTTTGAGGAAGTGAAGGCCTTTGTAAGAAGTGGGGTTTTTGGTCCCTATAACTATGAGGAGCTCATAGGATCTTTAGAGGGAAATGAAGGTTACGGTCGGGCAGACTACTTCCTCGTTGGCAAGGACTTCCCCAGTTACATAGAGTGCCAAGAGAGGGTTGATGAGGCATATCGAGATCAAAAGGTAAGCAACAGATTCCACACTCACTAATAAAGCTATGATGCTAATCTCCTAATCATCTACTTGTACTGGACAGAGATGGACCAAGATGTCGATCTTGAACACAGCTGGTTCATACAAGTTCAGCAGTGACAGAACCATTCATGAGTATGCAAGAGACATATGGAAGATTTCTCCACTAGTGCTATCTTAAAAGGTTCTGTTTCTTTCCTTTTTCCCGTAAAATTTGTTCTCCAACTCATTACTATTGTAATTGCCAAATGAGATGGGAAGATAGAAACAGGAGGTGAAAGTGAATCTCGTAGCCTATTCAAAATTAAAGCTACAACCAGAGAGAGGTATTTGATTACATAAGAAGTTCGTCAATGAAAATCTTAAACCCACTGGAACAGTCCAATTGATTTGATGTTAAGAGTTGGTTAAGAACAAGAACTAGC

mRNA sequence

GAAACACAGCAATGGCGGCATTGCGATTATCATGGGCCTGCGCTCATTCTAATCCAGATTCAAAGTCCAAATTCGTTTCCAGCTTCACTTCAGAGTCCAGTTTTCGGACCAATTGGACGAGGCTTCTGTTGCTTAGAACCTCGGTCTCAAGTTCTGCAAGAAGGAAATCGTGCATGAGGAACGTAGTCAGCGATCAACAGAAGGAACTAAACGAGCCGGTTAACGGAGAAGTTGTAGGCGGCTTCAATAGCTTGCTTCTGGATTCGGCATCCGTTGCCGCGAGTATTAAATACCATTCGGAGTTCACGCCTTCTTTCTCTCCGGAGGGCTTTGAGCTTTCTAAGGCATTCTATGCAACTGCTGAAAGTGTTCGTGATATGCTTATCATCAATTGGAATGCTACGTATGAATACTATGAAAAGATGAATGTGAAGCAAGCCTACTATTTGTCTATGGAGTTTCTACAGGGTAGAGCATTGCTAAATGCAATCGGAAATTTAGAGCTCTCTGGAGCTTATGCAGATGCTTTAAGAATGTTGGGATGTGATCTCGAGGATGTAGCAAGGCAGGAGTCGGATGCTGCATTAGGAAATGGAGGATTAGGGCGGCTTGCTTCCTGCTTTCTGGATTCCCTGGCAACCCTAAACTATCCTGCATGGGGCTATGGGCTCAGATACAAGTATGGTTTGTTCAAACAGCTCATTACAAAGGATGGGCAGGAGGAAGTTGCTGAAAATTGGCTAGAGATGGGTAATCCCTGGGAAATTGCAAGAAATGACGTCTTCTATCCTGTGAAATTCTATGGTGAAGTAATTTCAGGCGCAGATGGAAGTAAGCAATGGGTTGGAGGAGAAGATGTAACTGCTGTTGCCTATGATGTCCCAATACCAGGTTATAAAACTAAAAACACCATAAACCTCCGTCTGTGGTCCACAAAAGTTGCTCCGGAACAATTTAATTTAAGTTATTTCAATGTTGGAGATCATGCCAGTGCATATGCAGCCATAAAGAAAGCTGAGAAGATCTGCTACATATTGTACCCCGGAGATGAGTCACTGGAGGGAAAGACTCTTAGATTGAAACAACAATACACATTGTGCTCTGCTTCCCTTCAAGATATTGTTGCACGTTTTGAAAGGAGATCTGGAGAGCCTGTGGACTGGCAAAATTTTCCAGAAAAGGTTGCCGTACAGATGAATGATACGCACCCTACACTTTGCATCCCGGAGCTGATAAGAATATTGATGGATGTGAAAGGGTTGAGCTGGAAAGAAGCTTGGGACATCACTCGAAGAACTGTTGCGTACACAAATCATACTGTTCTGCCCGAGGCGCTGGAAAAATGGAGTTTCCCTCTCATGCAAGAATTGCTTCCTCGACATGTTGAGATAATTGAAATGATTGATGAGGAGCTTATCCACTCCATAGTTGCTCAGTACGGTACAAAAGACCTTGAGTTGTTGAAACAAAAACTGAAGCAAATGAGAATTCTAGAAAATTTTGAATTACCAGACTCGGTCATGGAAATGCTAGTAAAGTCAACGGAGGAGTCTGCAATCGATCTCGTTGAGGAAGCTGAAAATGTGGACGACGAATTGTTACCTAGTGAAGAAGGAGATGAATCTGAAGACAAAAATATTGAAAAGAAAACAGAGGTTTCGTTTGGAGTTGATCCAAAGCATCCAAGATTGATTCGAATGGCTAATTTATCTGTTGTTGGTGGATATGCTGTAAATGGTGTTGCAGAAATCCATAGTGAAATTGTTAGAACCGAAGTTTTCAGCGACTTCTATGAGTTGTGGCCTGAGAAATTTCAAAACAAAACAAATGGGGTGACACCGAGAAGATGGATTCGTTTTTGCAATCCGGACCTGAGTACAATCATCACAAAGTGGACTGGAACAGAGCAATGGGTGACAGATACTGAGAAACTGGCAATACTTAGAAAGTTTGCTGATAACGAGGATCTCCAGTCCATGTGGAAGGAAGCTAAAAGAATAAACAAATTGAAAGTTGTCTCTTTCCTTAAAGAAAAAACTGGCTACTTAGTCAGCCCTGATGCTATGTTTGACGTTCAGGTAAAACGCATCCACGAATACAAGCGGCAGTTGTTGAACATCATGGGGATCGTCTATCGTTATAAACAAATGAAAGAAATGAATATCGAGGAAGGAAAAGCCAAGTTTGTTCCTCGAGTCTGTATATTTGGTGGGAAAGCATTTGCTACGTATGTCCAAGCAAAAAGAATTGTAAAATTCATCACTGATGTTGGAGCTACCGTCAATAACGATCCTGATATAGGAGACCTTTTGAAGGTAGTCTTTGTTCCAGATTATAACGTGAGTGTAGCAGAAGTGCTCATTCCTGGCAGTGATCTATCCCAGCATATTAGCACGGCTGGAATGGAGGCCAGTGGAACCAGCAACATGAAGTTTGCGATGAATGGCTGCATACTAATTGGAACATTAGATGGAGCAAATGTGGAGATAAGAGAAGAGGTTGGAGAAGATAACTTTTTCCTTTTTGGTGCAAGGGCGCATGAAATTGCTGGCCTAAGAAAAGAAAGATCTCAAGGCAAGTTTGTTCCGGACCCGCGGTTTGAGGAAGTGAAGGCCTTTGTAAGAAGTGGGGTTTTTGGTCCCTATAACTATGAGGAGCTCATAGGATCTTTAGAGGGAAATGAAGGTTACGGTCGGGCAGACTACTTCCTCGTTGGCAAGGACTTCCCCAGTTACATAGAGTGCCAAGAGAGGGTTGATGAGGCATATCGAGATCAAAAGAGATGGACCAAGATGTCGATCTTGAACACAGCTGGTTCATACAAGTTCAGCAGTGACAGAACCATTCATGAGTATGCAAGAGACATATGGAAGATTTCTCCACTAGTGCTATCTTAAAAGGTTCTGTTTCTTTCCTTTTTCCCGTAAAATTTGTTCTCCAACTCATTACTATTGTAATTGCCAAATGAGATGGGAAGATAGAAACAGGAGGTGAAAGTGAATCTCGTAGCCTATTCAAAATTAAAGCTACAACCAGAGAGAGGTATTTGATTACATAAGAAGTTCGTCAATGAAAATCTTAAACCCACTGGAACAGTCCAATTGATTTGATGTTAAGAGTTGGTTAAGAACAAGAACTAGC

Coding sequence (CDS)

ATGGCGGCATTGCGATTATCATGGGCCTGCGCTCATTCTAATCCAGATTCAAAGTCCAAATTCGTTTCCAGCTTCACTTCAGAGTCCAGTTTTCGGACCAATTGGACGAGGCTTCTGTTGCTTAGAACCTCGGTCTCAAGTTCTGCAAGAAGGAAATCGTGCATGAGGAACGTAGTCAGCGATCAACAGAAGGAACTAAACGAGCCGGTTAACGGAGAAGTTGTAGGCGGCTTCAATAGCTTGCTTCTGGATTCGGCATCCGTTGCCGCGAGTATTAAATACCATTCGGAGTTCACGCCTTCTTTCTCTCCGGAGGGCTTTGAGCTTTCTAAGGCATTCTATGCAACTGCTGAAAGTGTTCGTGATATGCTTATCATCAATTGGAATGCTACGTATGAATACTATGAAAAGATGAATGTGAAGCAAGCCTACTATTTGTCTATGGAGTTTCTACAGGGTAGAGCATTGCTAAATGCAATCGGAAATTTAGAGCTCTCTGGAGCTTATGCAGATGCTTTAAGAATGTTGGGATGTGATCTCGAGGATGTAGCAAGGCAGGAGTCGGATGCTGCATTAGGAAATGGAGGATTAGGGCGGCTTGCTTCCTGCTTTCTGGATTCCCTGGCAACCCTAAACTATCCTGCATGGGGCTATGGGCTCAGATACAAGTATGGTTTGTTCAAACAGCTCATTACAAAGGATGGGCAGGAGGAAGTTGCTGAAAATTGGCTAGAGATGGGTAATCCCTGGGAAATTGCAAGAAATGACGTCTTCTATCCTGTGAAATTCTATGGTGAAGTAATTTCAGGCGCAGATGGAAGTAAGCAATGGGTTGGAGGAGAAGATGTAACTGCTGTTGCCTATGATGTCCCAATACCAGGTTATAAAACTAAAAACACCATAAACCTCCGTCTGTGGTCCACAAAAGTTGCTCCGGAACAATTTAATTTAAGTTATTTCAATGTTGGAGATCATGCCAGTGCATATGCAGCCATAAAGAAAGCTGAGAAGATCTGCTACATATTGTACCCCGGAGATGAGTCACTGGAGGGAAAGACTCTTAGATTGAAACAACAATACACATTGTGCTCTGCTTCCCTTCAAGATATTGTTGCACGTTTTGAAAGGAGATCTGGAGAGCCTGTGGACTGGCAAAATTTTCCAGAAAAGGTTGCCGTACAGATGAATGATACGCACCCTACACTTTGCATCCCGGAGCTGATAAGAATATTGATGGATGTGAAAGGGTTGAGCTGGAAAGAAGCTTGGGACATCACTCGAAGAACTGTTGCGTACACAAATCATACTGTTCTGCCCGAGGCGCTGGAAAAATGGAGTTTCCCTCTCATGCAAGAATTGCTTCCTCGACATGTTGAGATAATTGAAATGATTGATGAGGAGCTTATCCACTCCATAGTTGCTCAGTACGGTACAAAAGACCTTGAGTTGTTGAAACAAAAACTGAAGCAAATGAGAATTCTAGAAAATTTTGAATTACCAGACTCGGTCATGGAAATGCTAGTAAAGTCAACGGAGGAGTCTGCAATCGATCTCGTTGAGGAAGCTGAAAATGTGGACGACGAATTGTTACCTAGTGAAGAAGGAGATGAATCTGAAGACAAAAATATTGAAAAGAAAACAGAGGTTTCGTTTGGAGTTGATCCAAAGCATCCAAGATTGATTCGAATGGCTAATTTATCTGTTGTTGGTGGATATGCTGTAAATGGTGTTGCAGAAATCCATAGTGAAATTGTTAGAACCGAAGTTTTCAGCGACTTCTATGAGTTGTGGCCTGAGAAATTTCAAAACAAAACAAATGGGGTGACACCGAGAAGATGGATTCGTTTTTGCAATCCGGACCTGAGTACAATCATCACAAAGTGGACTGGAACAGAGCAATGGGTGACAGATACTGAGAAACTGGCAATACTTAGAAAGTTTGCTGATAACGAGGATCTCCAGTCCATGTGGAAGGAAGCTAAAAGAATAAACAAATTGAAAGTTGTCTCTTTCCTTAAAGAAAAAACTGGCTACTTAGTCAGCCCTGATGCTATGTTTGACGTTCAGGTAAAACGCATCCACGAATACAAGCGGCAGTTGTTGAACATCATGGGGATCGTCTATCGTTATAAACAAATGAAAGAAATGAATATCGAGGAAGGAAAAGCCAAGTTTGTTCCTCGAGTCTGTATATTTGGTGGGAAAGCATTTGCTACGTATGTCCAAGCAAAAAGAATTGTAAAATTCATCACTGATGTTGGAGCTACCGTCAATAACGATCCTGATATAGGAGACCTTTTGAAGGTAGTCTTTGTTCCAGATTATAACGTGAGTGTAGCAGAAGTGCTCATTCCTGGCAGTGATCTATCCCAGCATATTAGCACGGCTGGAATGGAGGCCAGTGGAACCAGCAACATGAAGTTTGCGATGAATGGCTGCATACTAATTGGAACATTAGATGGAGCAAATGTGGAGATAAGAGAAGAGGTTGGAGAAGATAACTTTTTCCTTTTTGGTGCAAGGGCGCATGAAATTGCTGGCCTAAGAAAAGAAAGATCTCAAGGCAAGTTTGTTCCGGACCCGCGGTTTGAGGAAGTGAAGGCCTTTGTAAGAAGTGGGGTTTTTGGTCCCTATAACTATGAGGAGCTCATAGGATCTTTAGAGGGAAATGAAGGTTACGGTCGGGCAGACTACTTCCTCGTTGGCAAGGACTTCCCCAGTTACATAGAGTGCCAAGAGAGGGTTGATGAGGCATATCGAGATCAAAAGAGATGGACCAAGATGTCGATCTTGAACACAGCTGGTTCATACAAGTTCAGCAGTGACAGAACCATTCATGAGTATGCAAGAGACATATGGAAGATTTCTCCACTAGTGCTATCTTAA

Protein sequence

MAALRLSWACAHSNPDSKSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVVSDQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESVRDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDLEDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVAENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNTINLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQYTLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWKEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTKDLELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPSEEGDESEDKNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSDFYELWPEKFQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNEDLQSMWKEAKRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQMKEMNIEEGKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFVPDYNVSVAEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFGARAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEGYGRADYFLVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLVLS
Homology
BLAST of MC09g1625 vs. ExPASy Swiss-Prot
Match: P53535 (Alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic OS=Solanum tuberosum OX=4113 GN=STP-1 PE=1 SV=1)

HSP 1 Score: 1465.7 bits (3793), Expect = 0.0e+00
Identity = 726/962 (75.47%), Postives = 826/962 (85.86%), Query Frame = 0

Query: 22  VSSFTS-ESSFRTNWTRLLLLRTSV---SSSARRKSCMRNVVSDQQKELNEPVNGEVVGG 81
           +SS +S  ++FR+  + +LL R  +   S   RR+S   + V+  QK+  +  + +    
Sbjct: 12  ISSISSFNNNFRSKNSNILLSRRRILLFSFRRRRRSFSVSSVASDQKQKTKDSSSDEGFT 71

Query: 82  FNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESVRDMLIINWNATYEYYEK 141
            +    DS SV +SIKYH+EFTPSFSPE FEL KA+YATAESVRD LIINWNATYE+YEK
Sbjct: 72  LDVFQPDSTSVLSSIKYHAEFTPSFSPEKFELPKAYYATAESVRDTLIINWNATYEFYEK 131

Query: 142 MNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDLEDVARQESDAALGNGGL 201
           MNVKQAYYLSMEFLQGRALLNAIGNL L+G YADAL  LG  LEDVARQE DAALGNGGL
Sbjct: 132 MNVKQAYYLSMEFLQGRALLNAIGNLGLTGPYADALTKLGYSLEDVARQEPDAALGNGGL 191

Query: 202 GRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVAENWLEMGNPWEIARNDV 261
           GRLASCFLDS+ATLNYPAWGYGLRY+YGLFKQLITKDGQEEVAENWLEMGNPWEI RND+
Sbjct: 192 GRLASCFLDSMATLNYPAWGYGLRYQYGLFKQLITKDGQEEVAENWLEMGNPWEIVRNDI 251

Query: 262 FYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNTINLRLWSTKVAPEQFNL 321
            YPVKFYG+VI GADG K+W GGED+TAVAYDVPIPGYKTK TINLRLW+TK+A E F+L
Sbjct: 252 SYPVKFYGKVIEGADGRKEWAGGEDITAVAYDVPIPGYKTKTTINLRLWTTKLAAEAFDL 311

Query: 322 SYFNVGDHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQYTLCSASLQDIVARFERR 381
             FN GDHA AY A KKAEKICY+LYPGDESLEGKTLRLKQQYTLCSASLQDI+ARFE+R
Sbjct: 312 YAFNNGDHAKAYEAQKKAEKICYVLYPGDESLEGKTLRLKQQYTLCSASLQDIIARFEKR 371

Query: 382 SGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWKEAWDITRRTVAYTNHTV 441
           SG  V+W  FPEKVAVQMNDTHPTLCIPEL+RILMDVKGLSWK+AW+IT+RTVAYTNHTV
Sbjct: 372 SGNAVNWDQFPEKVAVQMNDTHPTLCIPELLRILMDVKGLSWKQAWEITQRTVAYTNHTV 431

Query: 442 LPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTKDLELLKQKLKQMRILENF 501
           LPEALEKWSF L+ ELLPRHVEII MIDEEL+H+I+A+YGT+DL+LL++KL QMRIL+N 
Sbjct: 432 LPEALEKWSFTLLGELLPRHVEIIAMIDEELLHTILAEYGTEDLDLLQEKLNQMRILDNV 491

Query: 502 ELPDSVMEMLVKSTE-----ESAIDLVEEAENVDDELLPSEEGDESEDKNIEKKTEVS-- 561
           E+P SV+E+L+K+ E     E A D  +E E  DD      E  ++E  N E++TEV   
Sbjct: 492 EIPSSVLELLIKAEESAADVEKAADEEQEEEGKDDSKDEETEAVKAETTNEEEETEVKKV 551

Query: 562 ------------FGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSDFYELWP 621
                       FG  P  P+++ MANL VV G+AVNGVAEIHSEIV+ EVF++FY+LWP
Sbjct: 552 EVEDSQAKIKRIFGPHPNKPQVVHMANLCVVSGHAVNGVAEIHSEIVKDEVFNEFYKLWP 611

Query: 622 EKFQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNEDLQSMWK 681
           EKFQNKTNGVTPRRW+ FCNP+LS IITKWTG++ W+ +TEKLA LRKFADNE+LQS W+
Sbjct: 612 EKFQNKTNGVTPRRWLSFCNPELSEIITKWTGSDDWLVNTEKLAELRKFADNEELQSEWR 671

Query: 682 EAKRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQMKEMNI 741
           +AK  NK+K+VS +KEKTGY+VSPDAMFDVQ+KRIHEYKRQLLNI GIVYRYK+MKEM+ 
Sbjct: 672 KAKGNNKMKIVSLIKEKTGYVVSPDAMFDVQIKRIHEYKRQLLNIFGIVYRYKKMKEMSP 731

Query: 742 EEGKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFVPDYNVS 801
           EE K KFVPRVCIFGGKAFATYVQAKRIVKFITDVG TVN+DP+IGDLLKVVFVPDYNVS
Sbjct: 732 EERKEKFVPRVCIFGGKAFATYVQAKRIVKFITDVGETVNHDPEIGDLLKVVFVPDYNVS 791

Query: 802 VAEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLF 861
           VAEVLIPGS+LSQHISTAGMEASGTSNMKF+MNGC+LIGTLDGANVEIREEVGEDNFFLF
Sbjct: 792 VAEVLIPGSELSQHISTAGMEASGTSNMKFSMNGCLLIGTLDGANVEIREEVGEDNFFLF 851

Query: 862 GARAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEGYGRADY 921
           GA+AHEIAGLRKER++GKFVPDPRFEEVKAF+R+GVFG YNYEEL+GSLEGNEGYGRADY
Sbjct: 852 GAQAHEIAGLRKERAEGKFVPDPRFEEVKAFIRTGVFGTYNYEELMGSLEGNEGYGRADY 911

Query: 922 FLVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPL 961
           FLVGKDFP YIECQ++VDEAYRDQK+WTKMSILNTAGS+KFSSDRTIH+YARDIW+I P+
Sbjct: 912 FLVGKDFPDYIECQDKVDEAYRDQKKWTKMSILNTAGSFKFSSDRTIHQYARDIWRIEPV 971

BLAST of MC09g1625 vs. ExPASy Swiss-Prot
Match: P27598 (Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic OS=Ipomoea batatas OX=4120 PE=2 SV=1)

HSP 1 Score: 1404.0 bits (3633), Expect = 0.0e+00
Identity = 685/928 (73.81%), Postives = 792/928 (85.34%), Query Frame = 0

Query: 42  RTSVSSSARRKSCMRNVVSDQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPS 101
           RT+     +R   ++ V+ + ++ +   V  +  G     LLD+AS+A+SIKYH+EF+P+
Sbjct: 30  RTAGLQRTKRTLLVKCVLDETKQTIQHVVTEKNEG----TLLDAASIASSIKYHAEFSPA 89

Query: 102 FSPEGFELSKAFYATAESVRDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIG 161
           FSPE FEL KA++ATA+SVRD LI+NWNATY+YYEK+N+KQAYYLSMEFLQGRALLNAIG
Sbjct: 90  FSPERFELPKAYFATAQSVRDALIVNWNATYDYYEKLNMKQAYYLSMEFLQGRALLNAIG 149

Query: 162 NLELSGAYADALRMLGCDLEDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLR 221
           NLEL+G YA+AL  LG +LE+VA +E DAALGNGGLGRLASCFLDSLATLNYPAWGYGLR
Sbjct: 150 NLELTGEYAEALNKLGHNLENVASKEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLR 209

Query: 222 YKYGLFKQLITKDGQEEVAENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGE 281
           YKYGLFKQ ITKDGQEEVAE+WLE+GNPWEI R DV YPVKF+G+VI+G+DG K W+GGE
Sbjct: 210 YKYGLFKQRITKDGQEEVAEDWLELGNPWEIIRMDVSYPVKFFGKVITGSDGKKHWIGGE 269

Query: 282 DVTAVAYDVPIPGYKTKNTINLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYI 341
           D+ AVAYDVPIPGYKT+ TI+LRLWSTKV  E F+L  FN G+H  A  A   AEKICYI
Sbjct: 270 DILAVAYDVPIPGYKTRTTISLRLWSTKVPSEDFDLYSFNAGEHTKACEAQANAEKICYI 329

Query: 342 LYPGDESLEGKTLRLKQQYTLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPT 401
           LYPGDES+EGK LRLKQQYTLCSASLQDI+ARFERRSGE V W+ FPEKVAVQMNDTHPT
Sbjct: 330 LYPGDESIEGKILRLKQQYTLCSASLQDIIARFERRSGEYVKWEEFPEKVAVQMNDTHPT 389

Query: 402 LCIPELIRILMDVKGLSWKEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEII 461
           LCIPELIRIL+D+KGLSWKEAW+IT+RTVAYTNHTVLPEALEKWS+ LM++LLPRH+EII
Sbjct: 390 LCIPELIRILIDLKGLSWKEAWNITQRTVAYTNHTVLPEALEKWSYELMEKLLPRHIEII 449

Query: 462 EMIDEELIHSIVAQYGTKDLELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEE 521
           EMIDE+LI+ IV++YGT DL++L++KL  MRILENF++P S+  +  K  E S +D  EE
Sbjct: 450 EMIDEQLINEIVSEYGTSDLDMLEKKLNDMRILENFDIPSSIANLFTKPKETSIVDPSEE 509

Query: 522 AE----------NVDDELLPSEEGDESEDKNIEKKTEVSFGVDPKHPRLIRMANLSVVGG 581
            E           V D+++   E DE E+K+ E + +      P  P+++RMANL VVGG
Sbjct: 510 VEVSGKVVTESVEVSDKVVTESEKDELEEKDTELEKDEDPVPAPIPPKMVRMANLCVVGG 569

Query: 582 YAVNGVAEIHSEIVRTEVFSDFYELWPEKFQNKTNGVTPRRWIRFCNPDLSTIITKWTGT 641
           +AVNGVAEIHS+IV+ +VF+DFY+LWPEKFQNKTNGVTPRRWIRFCNP LS IITKW GT
Sbjct: 570 HAVNGVAEIHSDIVKEDVFNDFYQLWPEKFQNKTNGVTPRRWIRFCNPALSNIITKWIGT 629

Query: 642 EQWVTDTEKLAILRKFADNEDLQSMWKEAKRINKLKVVSFLKEKTGYLVSPDAMFDVQVK 701
           E WV +TEKLA LRKFADNEDLQ  W+ AKR NK+KV SFLKE+TGY VSP+AMFD+QVK
Sbjct: 630 EDWVLNTEKLAELRKFADNEDLQIEWRAAKRSNKVKVASFLKERTGYSVSPNAMFDIQVK 689

Query: 702 RIHEYKRQLLNIMGIVYRYKQMKEMNIEEGKAKFVPRVCIFGGKAFATYVQAKRIVKFIT 761
           RIHEYKRQLLNI+GIVYRYKQMKEM+  E +AKFVPRVCIFGGKAFATYVQAKRI KFIT
Sbjct: 690 RIHEYKRQLLNILGIVYRYKQMKEMSAREREAKFVPRVCIFGGKAFATYVQAKRIAKFIT 749

Query: 762 DVGATVNNDPDIGDLLKVVFVPDYNVSVAEVLIPGSDLSQHISTAGMEASGTSNMKFAMN 821
           DVGAT+N+DP+IGDLLKV+FVPDYNVS AE+LIP S LSQHISTAGMEASG SNMKFAMN
Sbjct: 750 DVGATINHDPEIGDLLKVIFVPDYNVSAAELLIPASGLSQHISTAGMEASGQSNMKFAMN 809

Query: 822 GCILIGTLDGANVEIREEVGEDNFFLFGARAHEIAGLRKERSQGKFVPDPRFEEVKAFVR 881
           GCILIGTLDGANVEIR+EVGE+NFFLFGA AHEIAGLRKER++GKFVPD RFEEVK F++
Sbjct: 810 GCILIGTLDGANVEIRQEVGEENFFLFGAEAHEIAGLRKERAEGKFVPDERFEEVKEFIK 869

Query: 882 SGVFGPYNYEELIGSLEGNEGYGRADYFLVGKDFPSYIECQERVDEAYRDQKRWTKMSIL 941
            GVFG   Y+EL+GSLEGNEG+GR DYFLVGKDFPSYIECQE+VDEAYRDQK WT+MSIL
Sbjct: 870 RGVFGSNTYDELLGSLEGNEGFGRGDYFLVGKDFPSYIECQEKVDEAYRDQKIWTRMSIL 929

Query: 942 NTAGSYKFSSDRTIHEYARDIWKISPLV 960
           NTAGSYKFSSDRTIHEYA+DIW I P+V
Sbjct: 930 NTAGSYKFSSDRTIHEYAKDIWNIQPVV 953

BLAST of MC09g1625 vs. ExPASy Swiss-Prot
Match: P53536 (Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic OS=Vicia faba OX=3906 GN=PHO1 PE=2 SV=2)

HSP 1 Score: 1401.7 bits (3627), Expect = 0.0e+00
Identity = 692/932 (74.25%), Postives = 785/932 (84.23%), Query Frame = 0

Query: 62   QQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESVR 121
            +QK  ++ V  E     +S   D+ S+ +SIKYH+EFTP FSPE FEL +AF ATA+SVR
Sbjct: 71   KQKVKDQEVQQEAKTSPSSFAPDTTSIVSSIKYHAEFTPLFSPEKFELPQAFIATAQSVR 130

Query: 122  DMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDLE 181
            D LIINWNATY+YYEK+NVKQAYYLSMEFLQGRALLNAIGNLEL+G YA+AL  L   LE
Sbjct: 131  DALIINWNATYDYYEKLNVKQAYYLSMEFLQGRALLNAIGNLELTGPYAEALSQLSYKLE 190

Query: 182  DVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVAE 241
            DVA QE DAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQ ITKDGQEEVAE
Sbjct: 191  DVAHQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQRITKDGQEEVAE 250

Query: 242  NWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNTI 301
            +WLEMGNPWEI RNDV YPV+FYG+V+SG+DG K WVGGED+ AVA+DVPIPGYKT++TI
Sbjct: 251  DWLEMGNPWEIVRNDVSYPVRFYGKVVSGSDGKKHWVGGEDIKAVAHDVPIPGYKTRSTI 310

Query: 302  NLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQYT 361
            NLRLWSTK A E+F+L+ FN G H  A  A+  AEKICYILYPGDES+EGKTLRLKQQYT
Sbjct: 311  NLRLWSTKAASEEFDLNAFNSGRHTEASEALANAEKICYILYPGDESIEGKTLRLKQQYT 370

Query: 362  LCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWKE 421
            LCSASLQDI+ARFERRSG  V+W++FPEKVAVQMNDTHPTLCIPEL+RIL+D+KGLSWK+
Sbjct: 371  LCSASLQDIIARFERRSGASVNWEDFPEKVAVQMNDTHPTLCIPELMRILIDIKGLSWKD 430

Query: 422  AWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTKDL 481
            AW+IT+RTVAYTNHTVLPEALEKWS  LM++LLPRHVEIIEMIDEELI +I+A+YGT D 
Sbjct: 431  AWNITQRTVAYTNHTVLPEALEKWSMDLMEKLLPRHVEIIEMIDEELIRTIIAEYGTADS 490

Query: 482  ELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVE---EAENVDDELLPSEEGDES 541
            +LL +KLK+MRILEN ELP    ++LVK+ E + I   E     E  ++E    E G+E 
Sbjct: 491  DLLDKKLKEMRILENVELPAEFADILVKTKEATDISSEEVQISKEGGEEEETSKEGGEEE 550

Query: 542  EDKNI--------------------EKKTEVSFGVD----------PKHPRLIRMANLSV 601
            E+K +                    EK   V   +           P  P+L+RMANL V
Sbjct: 551  EEKEVGGGREEGDDGKEDEVEKAIAEKDGTVKSSIGDKKKKLPEPVPVPPKLVRMANLCV 610

Query: 602  VGGYAVNGVAEIHSEIVRTEVFSDFYELWPEKFQNKTNGVTPRRWIRFCNPDLSTIITKW 661
            VGG+AVNGVAEIHSEIV+ +VF+ FY+LWPEKFQNKTNGVTPRRWIRFCNPDLS IIT+W
Sbjct: 611  VGGHAVNGVAEIHSEIVKDDVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDLSKIITQW 670

Query: 662  TGTEQWVTDTEKLAILRKFADNEDLQSMWKEAKRINKLKVVSFLKEKTGYLVSPDAMFDV 721
             GTE W+ +TEKLA LRKFADNEDLQ+ W+EAKR NK+KV +FL+E+TGY VSPD+MFD+
Sbjct: 671  IGTEDWILNTEKLAELRKFADNEDLQTQWREAKRNNKVKVAAFLRERTGYSVSPDSMFDI 730

Query: 722  QVKRIHEYKRQLLNIMGIVYRYKQMKEMNIEEGKAKFVPRVCIFGGKAFATYVQAKRIVK 781
            QVKRIHEYKRQLLNI GIVYRYK+MKEMN  E K  FVPRVCIFGGKAFATYVQAKRIVK
Sbjct: 731  QVKRIHEYKRQLLNIFGIVYRYKKMKEMNAAERKENFVPRVCIFGGKAFATYVQAKRIVK 790

Query: 782  FITDVGATVNNDPDIGDLLKVVFVPDYNVSVAEVLIPGSDLSQHISTAGMEASGTSNMKF 841
            FITDVGATVN+DP+IGDLLKV+FVPDYNVSVAE+LIP S+LSQHISTAGMEASGTSNMKF
Sbjct: 791  FITDVGATVNHDPEIGDLLKVIFVPDYNVSVAEMLIPASELSQHISTAGMEASGTSNMKF 850

Query: 842  AMNGCILIGTLDGANVEIREEVGEDNFFLFGARAHEIAGLRKERSQGKFVPDPRFEEVKA 901
            AMNGC+ IGTLDGANVEIREEVG DNFFLFGA+A EI GLRKER++GKFVPDPRFEEVK 
Sbjct: 851  AMNGCLQIGTLDGANVEIREEVGADNFFLFGAKAREIVGLRKERARGKFVPDPRFEEVKK 910

Query: 902  FVRSGVFGPYNYEELIGSLEGNEGYGRADYFLVGKDFPSYIECQERVDEAYRDQKRWTKM 961
            FVRSGVFG YNY+ELIGSLEGNEG+GRADYFLVG+DFPSY+ECQE VD+AYRDQK+WT+M
Sbjct: 911  FVRSGVFGSYNYDELIGSLEGNEGFGRADYFLVGQDFPSYLECQEEVDKAYRDQKKWTRM 970

BLAST of MC09g1625 vs. ExPASy Swiss-Prot
Match: P04045 (Alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic OS=Solanum tuberosum OX=4113 PE=1 SV=2)

HSP 1 Score: 1398.3 bits (3618), Expect = 0.0e+00
Identity = 702/963 (72.90%), Postives = 806/963 (83.70%), Query Frame = 0

Query: 17  SKSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVVSDQQKELNEPVNGEVVG 76
           S S+F+  FTS    R   ++L L +TS     +R   + N +S+   +++ P+  +  G
Sbjct: 15  SNSRFI-HFTS----RNTSSKLFLTKTSHFRRPKRCFHVNNTLSE---KIHHPITEQ--G 74

Query: 77  G---FNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESVRDMLIINWNATYE 136
           G    +S   D+AS+ +SIKYH+EFTP FSPE FEL KAF+ATA+SVRD L+INWNATY+
Sbjct: 75  GESDLSSFAPDAASITSSIKYHAEFTPVFSPERFELPKAFFATAQSVRDSLLINWNATYD 134

Query: 137 YYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDLEDVARQESDAALG 196
            YEK+N+KQAYYLSMEFLQGRALLNAIGNLEL+GA+A+AL+ LG +LE+VA QE DAALG
Sbjct: 135 IYEKLNMKQAYYLSMEFLQGRALLNAIGNLELTGAFAEALKNLGHNLENVASQEPDAALG 194

Query: 197 NGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVAENWLEMGNPWEIA 256
           NGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQ ITKDGQEEVAE+WLE+G+PWE+ 
Sbjct: 195 NGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQRITKDGQEEVAEDWLEIGSPWEVV 254

Query: 257 RNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNTINLRLWSTKVAPE 316
           RNDV YP+KFYG+V +G+DG + W+GGED+ AVAYDVPIPGYKT+ TI+LRLWST+V   
Sbjct: 255 RNDVSYPIKFYGKVSTGSDGKRYWIGGEDIKAVAYDVPIPGYKTRTTISLRLWSTQVPSA 314

Query: 317 QFNLSYFNVGDHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQYTLCSASLQDIVAR 376
            F+LS FN G+H  A  A   AEKICYILYPGDES EGK LRLKQQYTLCSASLQDI++R
Sbjct: 315 DFDLSAFNAGEHTKACEAQANAEKICYILYPGDESEEGKILRLKQQYTLCSASLQDIISR 374

Query: 377 FERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWKEAWDITRRTVAYT 436
           FERRSG+ + W+ FPEKVAVQMNDTHPTLCIPEL+RIL+D+KGL+W EAW+IT+RTVAYT
Sbjct: 375 FERRSGDRIKWEEFPEKVAVQMNDTHPTLCIPELMRILIDLKGLNWNEAWNITQRTVAYT 434

Query: 437 NHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTKDLELLKQKLKQMRI 496
           NHTVLPEALEKWS+ LMQ+LLPRHVEIIE IDEEL+H IV +YG+ DL  L++KL  MRI
Sbjct: 435 NHTVLPEALEKWSYELMQKLLPRHVEIIEAIDEELVHEIVLKYGSMDLNKLEEKLTTMRI 494

Query: 497 LENFELPDSVMEMLVKSTEESAIDLVEEAE-----NVDDELLPSEEGDESE--------- 556
           LENF+LP SV E+ +K  E S  D  E  E        D+++ ++E D  +         
Sbjct: 495 LENFDLPSSVAELFIK-PEISVDDDTETVEVHDKVEASDKVVTNDEDDTGKKTSVKIEAA 554

Query: 557 -DKNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSDFYELWP 616
            +K+I+KKT VS       P+ +RMANL VVGG+AVNGVAEIHSEIV+ EVF+DFYELWP
Sbjct: 555 AEKDIDKKTPVSPEPAVIPPKKVRMANLCVVGGHAVNGVAEIHSEIVKEEVFNDFYELWP 614

Query: 617 EKFQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNEDLQSMWK 676
           EKFQNKTNGVTPRRWIRFCNP LS IITKWTGTE WV  TEKLA L+KFADNEDLQ+ W+
Sbjct: 615 EKFQNKTNGVTPRRWIRFCNPPLSAIITKWTGTEDWVLKTEKLAELQKFADNEDLQNEWR 674

Query: 677 EAKRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQMKEMNI 736
           EAKR NK+KVVSFLKEKTGY V PDAMFD+QVKRIHEYKRQLLNI GIVYRYK+MKEM  
Sbjct: 675 EAKRSNKIKVVSFLKEKTGYSVVPDAMFDIQVKRIHEYKRQLLNIFGIVYRYKKMKEMTA 734

Query: 737 EEGKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFVPDYNVS 796
            E K  FVPRVCIFGGKAFATYVQAKRIVKFITDVGAT+N+DP+IGDLLKVVFVPDYNVS
Sbjct: 735 AERKTNFVPRVCIFGGKAFATYVQAKRIVKFITDVGATINHDPEIGDLLKVVFVPDYNVS 794

Query: 797 VAEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLF 856
           VAE+LIP SDLS+HISTAGMEASGTSNMKFAMNGCI IGTLDGANVEIREEVGE+NFFLF
Sbjct: 795 VAELLIPASDLSEHISTAGMEASGTSNMKFAMNGCIQIGTLDGANVEIREEVGEENFFLF 854

Query: 857 GARAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEGYGRADY 916
           GA+AHEIAGLRKER+ GKFVPD RFEEVK FVRSG FG YNY++LIGSLEGNEG+GRADY
Sbjct: 855 GAQAHEIAGLRKERADGKFVPDERFEEVKEFVRSGAFGSYNYDDLIGSLEGNEGFGRADY 914

Query: 917 FLVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPL 962
           FLVGKDFPSYIECQE+VDEAYRDQKRWT MSILNTAGSYKFSSDRTIHEYA+DIW I  +
Sbjct: 915 FLVGKDFPSYIECQEKVDEAYRDQKRWTTMSILNTAGSYKFSSDRTIHEYAKDIWNIEAV 966

BLAST of MC09g1625 vs. ExPASy Swiss-Prot
Match: Q9LIB2 (Alpha-glucan phosphorylase 1 OS=Arabidopsis thaliana OX=3702 GN=PHS1 PE=1 SV=1)

HSP 1 Score: 1361.7 bits (3523), Expect = 0.0e+00
Identity = 669/917 (72.96%), Postives = 775/917 (84.51%), Query Frame = 0

Query: 46  SSSARRKSCMRNVVSDQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPE 105
           S S+  K+ + + V D ++E+        +   N    D+ASVA+SIKYH+EFTP FSPE
Sbjct: 61  SISSEPKAKVTDAVLDSEQEV-------FISSMNPFAPDAASVASSIKYHAEFTPLFSPE 120

Query: 106 GFELSKAFYATAESVRDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLEL 165
            FEL KAF+ATA+SVRD LI+NWNATYEYY ++NVKQAYYLSMEFLQGRAL NA+GNL L
Sbjct: 121 KFELPKAFFATAQSVRDALIMNWNATYEYYNRVNVKQAYYLSMEFLQGRALSNAVGNLGL 180

Query: 166 SGAYADALRMLGCDLEDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYG 225
           + AY DAL+ LG DLE VA QE D ALGNGGLGRLASCFLDS+ATLNYPAWGYGLRYKYG
Sbjct: 181 NSAYGDALKRLGFDLESVASQEPDPALGNGGLGRLASCFLDSMATLNYPAWGYGLRYKYG 240

Query: 226 LFKQLITKDGQEEVAENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTA 285
           LFKQ ITKDGQEE AE+WLE+ NPWEI RNDV YP+KFYG+V+ G+DG K+W+GGED+ A
Sbjct: 241 LFKQRITKDGQEEAAEDWLELSNPWEIVRNDVSYPIKFYGKVVFGSDGKKRWIGGEDIVA 300

Query: 286 VAYDVPIPGYKTKNTINLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYILYPG 345
           VAYDVPIPGYKTK TINLRLWSTK   E F+LS +N G H  A  A+  AEKIC++LYPG
Sbjct: 301 VAYDVPIPGYKTKTTINLRLWSTKAPSEDFDLSSYNSGKHTEAAEALFNAEKICFVLYPG 360

Query: 346 DESLEGKTLRLKQQYTLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIP 405
           DES EGK LRLKQQYTLCSASLQDIVARFE RSG  V+W+ FPEKVAVQMNDTHPTLCIP
Sbjct: 361 DESTEGKALRLKQQYTLCSASLQDIVARFETRSGGNVNWEEFPEKVAVQMNDTHPTLCIP 420

Query: 406 ELIRILMDVKGLSWKEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMID 465
           EL+RILMD+KGLSW++AW IT+RTVAYTNHTVLPEALEKWS  LM++LLPRHVEIIE ID
Sbjct: 421 ELMRILMDLKGLSWEDAWKITQRTVAYTNHTVLPEALEKWSLELMEKLLPRHVEIIEKID 480

Query: 466 EELIHSIVAQYGTKDLELLKQKLKQMRILENFELPDSVMEMLVKSTEE--SAIDLVEEAE 525
           EEL+ +IV++YGT D +LL++KLK MRILEN ELP +  +++VK   +  +A D     +
Sbjct: 481 EELVRTIVSEYGTADPDLLEEKLKAMRILENVELPSAFADVIVKPVNKPVTAKDAQNGVK 540

Query: 526 NVDDELLPSEEGDESEDKNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSE 585
              +E      G+E ED+ I + T     V+P  P+++RMANL+VVGG+AVNGVAEIHSE
Sbjct: 541 TEQEE--EKTAGEEEEDEVIPEPT-----VEP--PKMVRMANLAVVGGHAVNGVAEIHSE 600

Query: 586 IVRTEVFSDFYELWPEKFQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAI 645
           IV+ +VF+DF +LWPEKFQNKTNGVTPRRWIRFCNP LS IIT W GTE WV +TEK+A 
Sbjct: 601 IVKQDVFNDFVQLWPEKFQNKTNGVTPRRWIRFCNPYLSDIITNWIGTEDWVLNTEKVAE 660

Query: 646 LRKFADNEDLQSMWKEAKRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNI 705
           LRKFADNEDLQS W+ AK+ NKLKVVS +KE+TGY VSPDAMFD+Q+KRIHEYKRQLLNI
Sbjct: 661 LRKFADNEDLQSEWRAAKKKNKLKVVSLIKERTGYTVSPDAMFDIQIKRIHEYKRQLLNI 720

Query: 706 MGIVYRYKQMKEMNIEEGKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDI 765
           +GIVYRYK+MKEM+  E +  FVPRVCIFGGKAFATYVQAKRIVKFITDV +T+N+DP+I
Sbjct: 721 LGIVYRYKKMKEMSASEREKAFVPRVCIFGGKAFATYVQAKRIVKFITDVASTINHDPEI 780

Query: 766 GDLLKVVFVPDYNVSVAEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGAN 825
           GDLLKV+FVPDYNVSVAE+LIP S+LSQHISTAGMEASGTSNMKF+MNGC+LIGTLDGAN
Sbjct: 781 GDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFSMNGCVLIGTLDGAN 840

Query: 826 VEIREEVGEDNFFLFGARAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEEL 885
           VEIREEVGE+NFFLFGA+A +I  LRKER++GKFVPDP FEEVK FV SGVFG  +Y+EL
Sbjct: 841 VEIREEVGEENFFLFGAKADQIVNLRKERAEGKFVPDPTFEEVKKFVGSGVFGSNSYDEL 900

Query: 886 IGSLEGNEGYGRADYFLVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDR 945
           IGSLEGNEG+GRADYFLVGKDFPSYIECQE+VDEAYRDQKRWT+MSI+NTAGS+KFSSDR
Sbjct: 901 IGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEAYRDQKRWTRMSIMNTAGSFKFSSDR 960

Query: 946 TIHEYARDIWKISPLVL 961
           TIHEYA+DIW I  + L
Sbjct: 961 TIHEYAKDIWNIKQVEL 961

BLAST of MC09g1625 vs. NCBI nr
Match: XP_022149091.1 (alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic-like isoform X2 [Momordica charantia])

HSP 1 Score: 1922 bits (4980), Expect = 0.0
Identity = 961/961 (100.00%), Postives = 961/961 (100.00%), Query Frame = 0

Query: 1   MAALRLSWACAHSNPDSKSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVVS 60
           MAALRLSWACAHSNPDSKSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVVS
Sbjct: 1   MAALRLSWACAHSNPDSKSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVVS 60

Query: 61  DQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESV 120
           DQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESV
Sbjct: 61  DQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESV 120

Query: 121 RDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDL 180
           RDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDL
Sbjct: 121 RDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDL 180

Query: 181 EDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA 240
           EDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA
Sbjct: 181 EDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA 240

Query: 241 ENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNT 300
           ENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNT
Sbjct: 241 ENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNT 300

Query: 301 INLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQY 360
           INLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQY
Sbjct: 301 INLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQY 360

Query: 361 TLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWK 420
           TLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWK
Sbjct: 361 TLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWK 420

Query: 421 EAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTKD 480
           EAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTKD
Sbjct: 421 EAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTKD 480

Query: 481 LELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPSEEGDESED 540
           LELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPSEEGDESED
Sbjct: 481 LELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPSEEGDESED 540

Query: 541 KNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSDFYELWPEK 600
           KNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSDFYELWPEK
Sbjct: 541 KNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSDFYELWPEK 600

Query: 601 FQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNEDLQSMWKEA 660
           FQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNEDLQSMWKEA
Sbjct: 601 FQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNEDLQSMWKEA 660

Query: 661 KRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQMKEMNIEE 720
           KRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQMKEMNIEE
Sbjct: 661 KRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQMKEMNIEE 720

Query: 721 GKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFVPDYNVSVA 780
           GKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFVPDYNVSVA
Sbjct: 721 GKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFVPDYNVSVA 780

Query: 781 EVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFGA 840
           EVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFGA
Sbjct: 781 EVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFGA 840

Query: 841 RAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEGYGRADYFL 900
           RAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEGYGRADYFL
Sbjct: 841 RAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEGYGRADYFL 900

Query: 901 VGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLVL 960
           VGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLVL
Sbjct: 901 VGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLVL 960

BLAST of MC09g1625 vs. NCBI nr
Match: XP_022149090.1 (alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic-like isoform X1 [Momordica charantia])

HSP 1 Score: 1915 bits (4961), Expect = 0.0
Identity = 961/969 (99.17%), Postives = 961/969 (99.17%), Query Frame = 0

Query: 1   MAALRLSWACAHSNPDSKSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVVS 60
           MAALRLSWACAHSNPDSKSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVVS
Sbjct: 1   MAALRLSWACAHSNPDSKSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVVS 60

Query: 61  DQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESV 120
           DQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESV
Sbjct: 61  DQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESV 120

Query: 121 RDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDL 180
           RDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDL
Sbjct: 121 RDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDL 180

Query: 181 EDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA 240
           EDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA
Sbjct: 181 EDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA 240

Query: 241 ENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNT 300
           ENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNT
Sbjct: 241 ENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNT 300

Query: 301 INLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEK--------ICYILYPGDESLEGK 360
           INLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEK        ICYILYPGDESLEGK
Sbjct: 301 INLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKKILTLPCKICYILYPGDESLEGK 360

Query: 361 TLRLKQQYTLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILM 420
           TLRLKQQYTLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILM
Sbjct: 361 TLRLKQQYTLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILM 420

Query: 421 DVKGLSWKEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSI 480
           DVKGLSWKEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSI
Sbjct: 421 DVKGLSWKEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSI 480

Query: 481 VAQYGTKDLELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPS 540
           VAQYGTKDLELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPS
Sbjct: 481 VAQYGTKDLELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPS 540

Query: 541 EEGDESEDKNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSD 600
           EEGDESEDKNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSD
Sbjct: 541 EEGDESEDKNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSD 600

Query: 601 FYELWPEKFQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNED 660
           FYELWPEKFQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNED
Sbjct: 601 FYELWPEKFQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNED 660

Query: 661 LQSMWKEAKRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQ 720
           LQSMWKEAKRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQ
Sbjct: 661 LQSMWKEAKRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQ 720

Query: 721 MKEMNIEEGKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFV 780
           MKEMNIEEGKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFV
Sbjct: 721 MKEMNIEEGKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFV 780

Query: 781 PDYNVSVAEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGE 840
           PDYNVSVAEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGE
Sbjct: 781 PDYNVSVAEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGE 840

Query: 841 DNFFLFGARAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEG 900
           DNFFLFGARAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEG
Sbjct: 841 DNFFLFGARAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEG 900

Query: 901 YGRADYFLVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDI 960
           YGRADYFLVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDI
Sbjct: 901 YGRADYFLVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDI 960

BLAST of MC09g1625 vs. NCBI nr
Match: KAG6574049.1 (Alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1751 bits (4535), Expect = 0.0
Identity = 867/962 (90.12%), Postives = 916/962 (95.22%), Query Frame = 0

Query: 1   MAALRLSWACAHSNPDS-KSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVV 60
           MA LRLSW CAHSNP+S +SKF+S FT+ESSFR  WTRLLL RTSVSSSARRK C+RNV 
Sbjct: 1   MAGLRLSWTCAHSNPESSRSKFLSRFTAESSFRCTWTRLLLFRTSVSSSARRKLCIRNVA 60

Query: 61  SDQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAES 120
           +DQQKE+ E VNGEVV   ++   DS S+AASIKYHSEFTPSFSPEGF LSKAFYATAES
Sbjct: 61  NDQQKEMKERVNGEVVDDCDTFFPDSESIAASIKYHSEFTPSFSPEGFGLSKAFYATAES 120

Query: 121 VRDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCD 180
           VRD+LIINWNATY YYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGC+
Sbjct: 121 VRDLLIINWNATYNYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCN 180

Query: 181 LEDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEV 240
           LE+VA+QESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEV
Sbjct: 181 LEEVAQQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEV 240

Query: 241 AENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKN 300
           AENWLEMGNPWEI RND+ YPVKFYGEVISGADGSKQWVGGE+VTAVAYDVPIPGYKTK 
Sbjct: 241 AENWLEMGNPWEIPRNDISYPVKFYGEVISGADGSKQWVGGENVTAVAYDVPIPGYKTKT 300

Query: 301 TINLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQ 360
           TINLRLWSTKVAPEQF+L+ FNVGDHA+AYAAIKKAEKICY+LYPGDESLEGKTLRLKQQ
Sbjct: 301 TINLRLWSTKVAPEQFDLNSFNVGDHANAYAAIKKAEKICYVLYPGDESLEGKTLRLKQQ 360

Query: 361 YTLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSW 420
           YTLCSASLQDIVARFERRSGE VDW+NFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSW
Sbjct: 361 YTLCSASLQDIVARFERRSGESVDWENFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSW 420

Query: 421 KEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTK 480
           KEAWDITRRTVAYTNHTVLPEALEKWSFPLMQEL PRHV+IIEMID+ELIHSI+AQYGTK
Sbjct: 421 KEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELFPRHVQIIEMIDKELIHSIIAQYGTK 480

Query: 481 DLELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPSEEGDESE 540
           DLELL+QKLKQMRILENFELPDSVME+LVKS EE A+DLVEEAE++D+E LP +E DESE
Sbjct: 481 DLELLQQKLKQMRILENFELPDSVMELLVKSAEEPAVDLVEEAESIDEESLPDKE-DESE 540

Query: 541 DKNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSDFYELWPE 600
           DK    K + SF VDPKHPR+IRMANLSVVGG+AVNGVAEIHSEIVRTEVF+DFYELWPE
Sbjct: 541 DKVTVNKVDASFKVDPKHPRMIRMANLSVVGGHAVNGVAEIHSEIVRTEVFNDFYELWPE 600

Query: 601 KFQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNEDLQSMWKE 660
           KFQNKTNGVTPRRWIRFCNPDLS IITKWTGTE WVTDTEKLAILRKFADNEDLQSMWKE
Sbjct: 601 KFQNKTNGVTPRRWIRFCNPDLSKIITKWTGTEHWVTDTEKLAILRKFADNEDLQSMWKE 660

Query: 661 AKRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQMKEMNIE 720
           A+R NKLKVVSFL+EKTGYLVSPDAMFDVQ+KRIHEYKRQLLNI+G+VYRYKQMKEM +E
Sbjct: 661 ARRRNKLKVVSFLEEKTGYLVSPDAMFDVQIKRIHEYKRQLLNILGVVYRYKQMKEMTLE 720

Query: 721 EGKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFVPDYNVSV 780
           E +AKFVPRVCIFGGKAF+TYVQAKRIVKFI DVGATVNND DIGDLLKVVFVPDYNVSV
Sbjct: 721 EREAKFVPRVCIFGGKAFSTYVQAKRIVKFIVDVGATVNNDSDIGDLLKVVFVPDYNVSV 780

Query: 781 AEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFG 840
           AEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFG
Sbjct: 781 AEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFG 840

Query: 841 ARAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEGYGRADYF 900
           ARAHEIAGLRKER++GKFVPDPRFEEVK FVRSGVFGP+NYEEL+GSLEGNEG+GRADYF
Sbjct: 841 ARAHEIAGLRKERAEGKFVPDPRFEEVKDFVRSGVFGPHNYEELMGSLEGNEGFGRADYF 900

Query: 901 LVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLV 960
           LVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPL+
Sbjct: 901 LVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLM 960

BLAST of MC09g1625 vs. NCBI nr
Match: XP_022945944.1 (alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1751 bits (4535), Expect = 0.0
Identity = 867/962 (90.12%), Postives = 916/962 (95.22%), Query Frame = 0

Query: 1   MAALRLSWACAHSNPDS-KSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVV 60
           MA LRLSW CAHSNP+S +SKF+S FT+ESSFR  WTRLLL RTSVSSSARRK C+RNV 
Sbjct: 1   MAGLRLSWTCAHSNPESSRSKFLSRFTAESSFRCTWTRLLLFRTSVSSSARRKLCIRNVA 60

Query: 61  SDQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAES 120
           +DQQKE+ E VNGEVV   ++   DS S+AASIKYHSEFTPSFSPEGF LSKAFYATAES
Sbjct: 61  NDQQKEMKERVNGEVVDDCDTFFPDSESIAASIKYHSEFTPSFSPEGFGLSKAFYATAES 120

Query: 121 VRDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCD 180
           VRD+LIINWNATY YYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGC+
Sbjct: 121 VRDLLIINWNATYNYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCN 180

Query: 181 LEDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEV 240
           LE+VA+QESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEV
Sbjct: 181 LEEVAQQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEV 240

Query: 241 AENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKN 300
           AENWLEMGNPWEI RND+ YPVKFYGEVISGADGSKQWVGGE+VTAVAYDVPIPGYKTK 
Sbjct: 241 AENWLEMGNPWEIPRNDISYPVKFYGEVISGADGSKQWVGGENVTAVAYDVPIPGYKTKT 300

Query: 301 TINLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQ 360
           TINLRLWSTKVAPEQF+L+ FNVGDHA+AYAAIKKAEKICY+LYPGDESLEGKTLRLKQQ
Sbjct: 301 TINLRLWSTKVAPEQFDLNSFNVGDHANAYAAIKKAEKICYVLYPGDESLEGKTLRLKQQ 360

Query: 361 YTLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSW 420
           YTLCSASLQDIVARFERRSGE VDW+NFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSW
Sbjct: 361 YTLCSASLQDIVARFERRSGESVDWENFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSW 420

Query: 421 KEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTK 480
           KEAWDITRRTVAYTNHTVLPEALEKWSFPLMQEL PRHV+IIEMID+ELIHSI+AQYGTK
Sbjct: 421 KEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELFPRHVQIIEMIDKELIHSIIAQYGTK 480

Query: 481 DLELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPSEEGDESE 540
           DLELL+QKLKQMRILENFELPDSVME+LVKS EE A+DLVEEAE++D+E LP +E DESE
Sbjct: 481 DLELLQQKLKQMRILENFELPDSVMELLVKSAEEPAVDLVEEAESIDEESLPDKE-DESE 540

Query: 541 DKNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSDFYELWPE 600
           DK    K + SF VDPKHPR+IRMANLSVVGG+AVNGVAEIHSEIVRTEVF+DFYELWPE
Sbjct: 541 DKVTVNKVDASFKVDPKHPRMIRMANLSVVGGHAVNGVAEIHSEIVRTEVFNDFYELWPE 600

Query: 601 KFQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNEDLQSMWKE 660
           KFQNKTNGVTPRRWIRFCNPDLS IITKWTGTE WVTDTEKLAILRKFADNEDLQSMWKE
Sbjct: 601 KFQNKTNGVTPRRWIRFCNPDLSKIITKWTGTEHWVTDTEKLAILRKFADNEDLQSMWKE 660

Query: 661 AKRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQMKEMNIE 720
           A+R NKLKVVSFL+EKTGYLVSPDAMFDVQ+KRIHEYKRQLLNI+G+VYRYKQMKEM +E
Sbjct: 661 ARRRNKLKVVSFLEEKTGYLVSPDAMFDVQIKRIHEYKRQLLNILGVVYRYKQMKEMTLE 720

Query: 721 EGKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFVPDYNVSV 780
           E +AKFVPRVCIFGGKAF+TYVQAKRIVKFI DVGATVNND DIGDLLKVVFVPDYNVSV
Sbjct: 721 EREAKFVPRVCIFGGKAFSTYVQAKRIVKFIVDVGATVNNDSDIGDLLKVVFVPDYNVSV 780

Query: 781 AEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFG 840
           AEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFG
Sbjct: 781 AEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFG 840

Query: 841 ARAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEGYGRADYF 900
           ARAHEIAGLRKER++GKFVPDPRFEEVK FVRSGVFGP+NYEEL+GSLEGNEG+GRADYF
Sbjct: 841 ARAHEIAGLRKERAEGKFVPDPRFEEVKDFVRSGVFGPHNYEELMGSLEGNEGFGRADYF 900

Query: 901 LVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLV 960
           LVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPL+
Sbjct: 901 LVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLL 960

BLAST of MC09g1625 vs. NCBI nr
Match: XP_022968480.1 (alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1747 bits (4525), Expect = 0.0
Identity = 867/961 (90.22%), Postives = 913/961 (95.01%), Query Frame = 0

Query: 1   MAALRLSWACAHSNPDSKSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVVS 60
           MA LRLSW CAHSNP+S SKF+S FT+ESSFR  WTRL L RTSVSSSARRK C+RNV +
Sbjct: 1   MAGLRLSWTCAHSNPES-SKFLSRFTAESSFRCTWTRLPLFRTSVSSSARRKLCIRNVAN 60

Query: 61  DQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESV 120
           DQQKEL E VNGEVV   ++   DS S+AASIKYHSEFTPSFSPEGF LSKAFYATAESV
Sbjct: 61  DQQKELKERVNGEVVDDCDTFFPDSESIAASIKYHSEFTPSFSPEGFGLSKAFYATAESV 120

Query: 121 RDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDL 180
           RDMLIINWNATY YYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGC+L
Sbjct: 121 RDMLIINWNATYNYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCNL 180

Query: 181 EDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA 240
           E+VA QESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA
Sbjct: 181 EEVAEQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA 240

Query: 241 ENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNT 300
           ENWLEMGNPWEI RND+ YPVKFYGEVISGADGSKQWVGGE+VTAVAYDVPIPGYKTK T
Sbjct: 241 ENWLEMGNPWEIPRNDISYPVKFYGEVISGADGSKQWVGGENVTAVAYDVPIPGYKTKTT 300

Query: 301 INLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQY 360
           INLRLWSTKVAPEQF+L+ FNVGDHA+AYAAIKKAEKICY+LYPGDESLEGKTLRLKQQY
Sbjct: 301 INLRLWSTKVAPEQFDLNSFNVGDHANAYAAIKKAEKICYVLYPGDESLEGKTLRLKQQY 360

Query: 361 TLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWK 420
           TLCSASLQDIVARFERRSGE VDW+NFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWK
Sbjct: 361 TLCSASLQDIVARFERRSGESVDWENFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWK 420

Query: 421 EAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTKD 480
           EAWDITRRTVAYTNHTVLPEALEKWSFPLMQEL PRHV+IIEMID+ELIHSI+AQYGTKD
Sbjct: 421 EAWDITRRTVAYTNHTVLPEALEKWSFPLMQELFPRHVQIIEMIDKELIHSIIAQYGTKD 480

Query: 481 LELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPSEEGDESED 540
           LELL+QKLK+MRILENFELPDSVME+LVKS EE A+DLVEEAE++D+E LP +E DESED
Sbjct: 481 LELLQQKLKKMRILENFELPDSVMELLVKSAEEPAVDLVEEAESIDEESLPDKE-DESED 540

Query: 541 KNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSDFYELWPEK 600
           K    K + SF VDPKHPR+IRMANLSVVGG+AVNGVAEIHSEIVRTEVFSDFYELWPEK
Sbjct: 541 KVTVNKVDASFKVDPKHPRMIRMANLSVVGGHAVNGVAEIHSEIVRTEVFSDFYELWPEK 600

Query: 601 FQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNEDLQSMWKEA 660
           FQNKTNGVTPRRWIRFCNPDLS IITKWTGTE WVTDTEKLAILRKFADNEDLQS+WKEA
Sbjct: 601 FQNKTNGVTPRRWIRFCNPDLSRIITKWTGTEHWVTDTEKLAILRKFADNEDLQSIWKEA 660

Query: 661 KRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQMKEMNIEE 720
           +R NKLKVVSFL+EKTGYLVSPDAMFDVQ+KRIHEYKRQLLNI+G+VYRYKQMKEM +EE
Sbjct: 661 RRRNKLKVVSFLEEKTGYLVSPDAMFDVQIKRIHEYKRQLLNILGVVYRYKQMKEMTLEE 720

Query: 721 GKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFVPDYNVSVA 780
            +AKFVPRVCIFGGKAF+TYVQAKRIVKFI DVGATVNND DIGDLLKVVFVPDYNVSVA
Sbjct: 721 REAKFVPRVCIFGGKAFSTYVQAKRIVKFIVDVGATVNNDSDIGDLLKVVFVPDYNVSVA 780

Query: 781 EVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFGA 840
           EVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFGA
Sbjct: 781 EVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFGA 840

Query: 841 RAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEGYGRADYFL 900
           RAHEIAGLRKER++GKFVPDPRFEEVK FVRSGVFGP+NYEEL+GSLEGNEG+GRADYFL
Sbjct: 841 RAHEIAGLRKERAEGKFVPDPRFEEVKDFVRSGVFGPHNYEELMGSLEGNEGFGRADYFL 900

Query: 901 VGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLVL 960
           VGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPL+L
Sbjct: 901 VGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLLL 959

BLAST of MC09g1625 vs. ExPASy TrEMBL
Match: A0A6J1D7B6 (Alpha-1,4 glucan phosphorylase OS=Momordica charantia OX=3673 GN=LOC111017587 PE=3 SV=1)

HSP 1 Score: 1922 bits (4980), Expect = 0.0
Identity = 961/961 (100.00%), Postives = 961/961 (100.00%), Query Frame = 0

Query: 1   MAALRLSWACAHSNPDSKSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVVS 60
           MAALRLSWACAHSNPDSKSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVVS
Sbjct: 1   MAALRLSWACAHSNPDSKSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVVS 60

Query: 61  DQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESV 120
           DQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESV
Sbjct: 61  DQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESV 120

Query: 121 RDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDL 180
           RDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDL
Sbjct: 121 RDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDL 180

Query: 181 EDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA 240
           EDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA
Sbjct: 181 EDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA 240

Query: 241 ENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNT 300
           ENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNT
Sbjct: 241 ENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNT 300

Query: 301 INLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQY 360
           INLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQY
Sbjct: 301 INLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQY 360

Query: 361 TLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWK 420
           TLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWK
Sbjct: 361 TLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWK 420

Query: 421 EAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTKD 480
           EAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTKD
Sbjct: 421 EAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTKD 480

Query: 481 LELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPSEEGDESED 540
           LELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPSEEGDESED
Sbjct: 481 LELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPSEEGDESED 540

Query: 541 KNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSDFYELWPEK 600
           KNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSDFYELWPEK
Sbjct: 541 KNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSDFYELWPEK 600

Query: 601 FQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNEDLQSMWKEA 660
           FQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNEDLQSMWKEA
Sbjct: 601 FQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNEDLQSMWKEA 660

Query: 661 KRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQMKEMNIEE 720
           KRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQMKEMNIEE
Sbjct: 661 KRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQMKEMNIEE 720

Query: 721 GKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFVPDYNVSVA 780
           GKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFVPDYNVSVA
Sbjct: 721 GKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFVPDYNVSVA 780

Query: 781 EVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFGA 840
           EVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFGA
Sbjct: 781 EVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFGA 840

Query: 841 RAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEGYGRADYFL 900
           RAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEGYGRADYFL
Sbjct: 841 RAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEGYGRADYFL 900

Query: 901 VGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLVL 960
           VGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLVL
Sbjct: 901 VGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLVL 960

BLAST of MC09g1625 vs. ExPASy TrEMBL
Match: A0A6J1D4S6 (Alpha-1,4 glucan phosphorylase OS=Momordica charantia OX=3673 GN=LOC111017587 PE=3 SV=1)

HSP 1 Score: 1915 bits (4961), Expect = 0.0
Identity = 961/969 (99.17%), Postives = 961/969 (99.17%), Query Frame = 0

Query: 1   MAALRLSWACAHSNPDSKSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVVS 60
           MAALRLSWACAHSNPDSKSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVVS
Sbjct: 1   MAALRLSWACAHSNPDSKSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVVS 60

Query: 61  DQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESV 120
           DQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESV
Sbjct: 61  DQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESV 120

Query: 121 RDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDL 180
           RDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDL
Sbjct: 121 RDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDL 180

Query: 181 EDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA 240
           EDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA
Sbjct: 181 EDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA 240

Query: 241 ENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNT 300
           ENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNT
Sbjct: 241 ENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNT 300

Query: 301 INLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEK--------ICYILYPGDESLEGK 360
           INLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEK        ICYILYPGDESLEGK
Sbjct: 301 INLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKKILTLPCKICYILYPGDESLEGK 360

Query: 361 TLRLKQQYTLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILM 420
           TLRLKQQYTLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILM
Sbjct: 361 TLRLKQQYTLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILM 420

Query: 421 DVKGLSWKEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSI 480
           DVKGLSWKEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSI
Sbjct: 421 DVKGLSWKEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSI 480

Query: 481 VAQYGTKDLELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPS 540
           VAQYGTKDLELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPS
Sbjct: 481 VAQYGTKDLELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPS 540

Query: 541 EEGDESEDKNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSD 600
           EEGDESEDKNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSD
Sbjct: 541 EEGDESEDKNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSD 600

Query: 601 FYELWPEKFQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNED 660
           FYELWPEKFQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNED
Sbjct: 601 FYELWPEKFQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNED 660

Query: 661 LQSMWKEAKRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQ 720
           LQSMWKEAKRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQ
Sbjct: 661 LQSMWKEAKRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQ 720

Query: 721 MKEMNIEEGKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFV 780
           MKEMNIEEGKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFV
Sbjct: 721 MKEMNIEEGKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFV 780

Query: 781 PDYNVSVAEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGE 840
           PDYNVSVAEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGE
Sbjct: 781 PDYNVSVAEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGE 840

Query: 841 DNFFLFGARAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEG 900
           DNFFLFGARAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEG
Sbjct: 841 DNFFLFGARAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEG 900

Query: 901 YGRADYFLVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDI 960
           YGRADYFLVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDI
Sbjct: 901 YGRADYFLVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDI 960

BLAST of MC09g1625 vs. ExPASy TrEMBL
Match: A0A6J1G2D1 (Alpha-1,4 glucan phosphorylase OS=Cucurbita moschata OX=3662 GN=LOC111450034 PE=3 SV=1)

HSP 1 Score: 1751 bits (4535), Expect = 0.0
Identity = 867/962 (90.12%), Postives = 916/962 (95.22%), Query Frame = 0

Query: 1   MAALRLSWACAHSNPDS-KSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVV 60
           MA LRLSW CAHSNP+S +SKF+S FT+ESSFR  WTRLLL RTSVSSSARRK C+RNV 
Sbjct: 1   MAGLRLSWTCAHSNPESSRSKFLSRFTAESSFRCTWTRLLLFRTSVSSSARRKLCIRNVA 60

Query: 61  SDQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAES 120
           +DQQKE+ E VNGEVV   ++   DS S+AASIKYHSEFTPSFSPEGF LSKAFYATAES
Sbjct: 61  NDQQKEMKERVNGEVVDDCDTFFPDSESIAASIKYHSEFTPSFSPEGFGLSKAFYATAES 120

Query: 121 VRDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCD 180
           VRD+LIINWNATY YYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGC+
Sbjct: 121 VRDLLIINWNATYNYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCN 180

Query: 181 LEDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEV 240
           LE+VA+QESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEV
Sbjct: 181 LEEVAQQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEV 240

Query: 241 AENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKN 300
           AENWLEMGNPWEI RND+ YPVKFYGEVISGADGSKQWVGGE+VTAVAYDVPIPGYKTK 
Sbjct: 241 AENWLEMGNPWEIPRNDISYPVKFYGEVISGADGSKQWVGGENVTAVAYDVPIPGYKTKT 300

Query: 301 TINLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQ 360
           TINLRLWSTKVAPEQF+L+ FNVGDHA+AYAAIKKAEKICY+LYPGDESLEGKTLRLKQQ
Sbjct: 301 TINLRLWSTKVAPEQFDLNSFNVGDHANAYAAIKKAEKICYVLYPGDESLEGKTLRLKQQ 360

Query: 361 YTLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSW 420
           YTLCSASLQDIVARFERRSGE VDW+NFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSW
Sbjct: 361 YTLCSASLQDIVARFERRSGESVDWENFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSW 420

Query: 421 KEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTK 480
           KEAWDITRRTVAYTNHTVLPEALEKWSFPLMQEL PRHV+IIEMID+ELIHSI+AQYGTK
Sbjct: 421 KEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELFPRHVQIIEMIDKELIHSIIAQYGTK 480

Query: 481 DLELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPSEEGDESE 540
           DLELL+QKLKQMRILENFELPDSVME+LVKS EE A+DLVEEAE++D+E LP +E DESE
Sbjct: 481 DLELLQQKLKQMRILENFELPDSVMELLVKSAEEPAVDLVEEAESIDEESLPDKE-DESE 540

Query: 541 DKNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSDFYELWPE 600
           DK    K + SF VDPKHPR+IRMANLSVVGG+AVNGVAEIHSEIVRTEVF+DFYELWPE
Sbjct: 541 DKVTVNKVDASFKVDPKHPRMIRMANLSVVGGHAVNGVAEIHSEIVRTEVFNDFYELWPE 600

Query: 601 KFQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNEDLQSMWKE 660
           KFQNKTNGVTPRRWIRFCNPDLS IITKWTGTE WVTDTEKLAILRKFADNEDLQSMWKE
Sbjct: 601 KFQNKTNGVTPRRWIRFCNPDLSKIITKWTGTEHWVTDTEKLAILRKFADNEDLQSMWKE 660

Query: 661 AKRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQMKEMNIE 720
           A+R NKLKVVSFL+EKTGYLVSPDAMFDVQ+KRIHEYKRQLLNI+G+VYRYKQMKEM +E
Sbjct: 661 ARRRNKLKVVSFLEEKTGYLVSPDAMFDVQIKRIHEYKRQLLNILGVVYRYKQMKEMTLE 720

Query: 721 EGKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFVPDYNVSV 780
           E +AKFVPRVCIFGGKAF+TYVQAKRIVKFI DVGATVNND DIGDLLKVVFVPDYNVSV
Sbjct: 721 EREAKFVPRVCIFGGKAFSTYVQAKRIVKFIVDVGATVNNDSDIGDLLKVVFVPDYNVSV 780

Query: 781 AEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFG 840
           AEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFG
Sbjct: 781 AEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFG 840

Query: 841 ARAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEGYGRADYF 900
           ARAHEIAGLRKER++GKFVPDPRFEEVK FVRSGVFGP+NYEEL+GSLEGNEG+GRADYF
Sbjct: 841 ARAHEIAGLRKERAEGKFVPDPRFEEVKDFVRSGVFGPHNYEELMGSLEGNEGFGRADYF 900

Query: 901 LVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLV 960
           LVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPL+
Sbjct: 901 LVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLL 960

BLAST of MC09g1625 vs. ExPASy TrEMBL
Match: A0A6J1HXA2 (Alpha-1,4 glucan phosphorylase OS=Cucurbita maxima OX=3661 GN=LOC111467708 PE=3 SV=1)

HSP 1 Score: 1747 bits (4525), Expect = 0.0
Identity = 867/961 (90.22%), Postives = 913/961 (95.01%), Query Frame = 0

Query: 1   MAALRLSWACAHSNPDSKSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVVS 60
           MA LRLSW CAHSNP+S SKF+S FT+ESSFR  WTRL L RTSVSSSARRK C+RNV +
Sbjct: 1   MAGLRLSWTCAHSNPES-SKFLSRFTAESSFRCTWTRLPLFRTSVSSSARRKLCIRNVAN 60

Query: 61  DQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESV 120
           DQQKEL E VNGEVV   ++   DS S+AASIKYHSEFTPSFSPEGF LSKAFYATAESV
Sbjct: 61  DQQKELKERVNGEVVDDCDTFFPDSESIAASIKYHSEFTPSFSPEGFGLSKAFYATAESV 120

Query: 121 RDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDL 180
           RDMLIINWNATY YYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGC+L
Sbjct: 121 RDMLIINWNATYNYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCNL 180

Query: 181 EDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA 240
           E+VA QESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA
Sbjct: 181 EEVAEQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVA 240

Query: 241 ENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNT 300
           ENWLEMGNPWEI RND+ YPVKFYGEVISGADGSKQWVGGE+VTAVAYDVPIPGYKTK T
Sbjct: 241 ENWLEMGNPWEIPRNDISYPVKFYGEVISGADGSKQWVGGENVTAVAYDVPIPGYKTKTT 300

Query: 301 INLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQY 360
           INLRLWSTKVAPEQF+L+ FNVGDHA+AYAAIKKAEKICY+LYPGDESLEGKTLRLKQQY
Sbjct: 301 INLRLWSTKVAPEQFDLNSFNVGDHANAYAAIKKAEKICYVLYPGDESLEGKTLRLKQQY 360

Query: 361 TLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWK 420
           TLCSASLQDIVARFERRSGE VDW+NFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWK
Sbjct: 361 TLCSASLQDIVARFERRSGESVDWENFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWK 420

Query: 421 EAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTKD 480
           EAWDITRRTVAYTNHTVLPEALEKWSFPLMQEL PRHV+IIEMID+ELIHSI+AQYGTKD
Sbjct: 421 EAWDITRRTVAYTNHTVLPEALEKWSFPLMQELFPRHVQIIEMIDKELIHSIIAQYGTKD 480

Query: 481 LELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPSEEGDESED 540
           LELL+QKLK+MRILENFELPDSVME+LVKS EE A+DLVEEAE++D+E LP +E DESED
Sbjct: 481 LELLQQKLKKMRILENFELPDSVMELLVKSAEEPAVDLVEEAESIDEESLPDKE-DESED 540

Query: 541 KNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSDFYELWPEK 600
           K    K + SF VDPKHPR+IRMANLSVVGG+AVNGVAEIHSEIVRTEVFSDFYELWPEK
Sbjct: 541 KVTVNKVDASFKVDPKHPRMIRMANLSVVGGHAVNGVAEIHSEIVRTEVFSDFYELWPEK 600

Query: 601 FQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRKFADNEDLQSMWKEA 660
           FQNKTNGVTPRRWIRFCNPDLS IITKWTGTE WVTDTEKLAILRKFADNEDLQS+WKEA
Sbjct: 601 FQNKTNGVTPRRWIRFCNPDLSRIITKWTGTEHWVTDTEKLAILRKFADNEDLQSIWKEA 660

Query: 661 KRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQMKEMNIEE 720
           +R NKLKVVSFL+EKTGYLVSPDAMFDVQ+KRIHEYKRQLLNI+G+VYRYKQMKEM +EE
Sbjct: 661 RRRNKLKVVSFLEEKTGYLVSPDAMFDVQIKRIHEYKRQLLNILGVVYRYKQMKEMTLEE 720

Query: 721 GKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFVPDYNVSVA 780
            +AKFVPRVCIFGGKAF+TYVQAKRIVKFI DVGATVNND DIGDLLKVVFVPDYNVSVA
Sbjct: 721 REAKFVPRVCIFGGKAFSTYVQAKRIVKFIVDVGATVNNDSDIGDLLKVVFVPDYNVSVA 780

Query: 781 EVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFGA 840
           EVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFGA
Sbjct: 781 EVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFGA 840

Query: 841 RAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEGYGRADYFL 900
           RAHEIAGLRKER++GKFVPDPRFEEVK FVRSGVFGP+NYEEL+GSLEGNEG+GRADYFL
Sbjct: 841 RAHEIAGLRKERAEGKFVPDPRFEEVKDFVRSGVFGPHNYEELMGSLEGNEGFGRADYFL 900

Query: 901 VGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLVL 960
           VGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPL+L
Sbjct: 901 VGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKISPLLL 959

BLAST of MC09g1625 vs. ExPASy TrEMBL
Match: A0A6J1G2F1 (Alpha-1,4 glucan phosphorylase OS=Cucurbita moschata OX=3662 GN=LOC111450034 PE=3 SV=1)

HSP 1 Score: 1746 bits (4521), Expect = 0.0
Identity = 867/965 (89.84%), Postives = 916/965 (94.92%), Query Frame = 0

Query: 1   MAALRLSWACAHSNPDS-KSKFVSSFTSESSFRTNWTRLLLLRTSVSSSARRKSCMRNVV 60
           MA LRLSW CAHSNP+S +SKF+S FT+ESSFR  WTRLLL RTSVSSSARRK C+RNV 
Sbjct: 1   MAGLRLSWTCAHSNPESSRSKFLSRFTAESSFRCTWTRLLLFRTSVSSSARRKLCIRNVA 60

Query: 61  SDQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPEGFELSKAFYATAES 120
           +DQQKE+ E VNGEVV   ++   DS S+AASIKYHSEFTPSFSPEGF LSKAFYATAES
Sbjct: 61  NDQQKEMKERVNGEVVDDCDTFFPDSESIAASIKYHSEFTPSFSPEGFGLSKAFYATAES 120

Query: 121 VRDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCD 180
           VRD+LIINWNATY YYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGC+
Sbjct: 121 VRDLLIINWNATYNYYEKMNVKQAYYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCN 180

Query: 181 LEDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEV 240
           LE+VA+QESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEV
Sbjct: 181 LEEVAQQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEV 240

Query: 241 AENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKN 300
           AENWLEMGNPWEI RND+ YPVKFYGEVISGADGSKQWVGGE+VTAVAYDVPIPGYKTK 
Sbjct: 241 AENWLEMGNPWEIPRNDISYPVKFYGEVISGADGSKQWVGGENVTAVAYDVPIPGYKTKT 300

Query: 301 TINLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQ 360
           TINLRLWSTKVAPEQF+L+ FNVGDHA+AYAAIKKAEKICY+LYPGDESLEGKTLRLKQQ
Sbjct: 301 TINLRLWSTKVAPEQFDLNSFNVGDHANAYAAIKKAEKICYVLYPGDESLEGKTLRLKQQ 360

Query: 361 YTLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSW 420
           YTLCSASLQDIVARFERRSGE VDW+NFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSW
Sbjct: 361 YTLCSASLQDIVARFERRSGESVDWENFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSW 420

Query: 421 KEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTK 480
           KEAWDITRRTVAYTNHTVLPEALEKWSFPLMQEL PRHV+IIEMID+ELIHSI+AQYGTK
Sbjct: 421 KEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELFPRHVQIIEMIDKELIHSIIAQYGTK 480

Query: 481 DLELLKQKLKQMRILENFELPDSVMEMLVKSTEESAIDLVEEAENVDDELLPSEEGDESE 540
           DLELL+QKLKQMRILENFELPDSVME+LVKS EE A+DLVEEAE++D+E LP +E DESE
Sbjct: 481 DLELLQQKLKQMRILENFELPDSVMELLVKSAEEPAVDLVEEAESIDEESLPDKE-DESE 540

Query: 541 DKNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSEIVRTEVFSDFYELWPE 600
           DK    K + SF VDPKHPR+IRMANLSVVGG+AVNGVAEIHSEIVRTEVF+DFYELWPE
Sbjct: 541 DKVTVNKVDASFKVDPKHPRMIRMANLSVVGGHAVNGVAEIHSEIVRTEVFNDFYELWPE 600

Query: 601 KFQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAILRK---FADNEDLQSM 660
           KFQNKTNGVTPRRWIRFCNPDLS IITKWTGTE WVTDTEKLAILRK   FADNEDLQSM
Sbjct: 601 KFQNKTNGVTPRRWIRFCNPDLSKIITKWTGTEHWVTDTEKLAILRKVKLFADNEDLQSM 660

Query: 661 WKEAKRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQMKEM 720
           WKEA+R NKLKVVSFL+EKTGYLVSPDAMFDVQ+KRIHEYKRQLLNI+G+VYRYKQMKEM
Sbjct: 661 WKEARRRNKLKVVSFLEEKTGYLVSPDAMFDVQIKRIHEYKRQLLNILGVVYRYKQMKEM 720

Query: 721 NIEEGKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDIGDLLKVVFVPDYN 780
            +EE +AKFVPRVCIFGGKAF+TYVQAKRIVKFI DVGATVNND DIGDLLKVVFVPDYN
Sbjct: 721 TLEEREAKFVPRVCIFGGKAFSTYVQAKRIVKFIVDVGATVNNDSDIGDLLKVVFVPDYN 780

Query: 781 VSVAEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFF 840
           VSVAEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFF
Sbjct: 781 VSVAEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFF 840

Query: 841 LFGARAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEELIGSLEGNEGYGRA 900
           LFGARAHEIAGLRKER++GKFVPDPRFEEVK FVRSGVFGP+NYEEL+GSLEGNEG+GRA
Sbjct: 841 LFGARAHEIAGLRKERAEGKFVPDPRFEEVKDFVRSGVFGPHNYEELMGSLEGNEGFGRA 900

Query: 901 DYFLVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKIS 960
           DYFLVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKIS
Sbjct: 901 DYFLVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKIS 960

BLAST of MC09g1625 vs. TAIR 10
Match: AT3G29320.1 (Glycosyl transferase, family 35 )

HSP 1 Score: 1361.7 bits (3523), Expect = 0.0e+00
Identity = 669/917 (72.96%), Postives = 775/917 (84.51%), Query Frame = 0

Query: 46  SSSARRKSCMRNVVSDQQKELNEPVNGEVVGGFNSLLLDSASVAASIKYHSEFTPSFSPE 105
           S S+  K+ + + V D ++E+        +   N    D+ASVA+SIKYH+EFTP FSPE
Sbjct: 61  SISSEPKAKVTDAVLDSEQEV-------FISSMNPFAPDAASVASSIKYHAEFTPLFSPE 120

Query: 106 GFELSKAFYATAESVRDMLIINWNATYEYYEKMNVKQAYYLSMEFLQGRALLNAIGNLEL 165
            FEL KAF+ATA+SVRD LI+NWNATYEYY ++NVKQAYYLSMEFLQGRAL NA+GNL L
Sbjct: 121 KFELPKAFFATAQSVRDALIMNWNATYEYYNRVNVKQAYYLSMEFLQGRALSNAVGNLGL 180

Query: 166 SGAYADALRMLGCDLEDVARQESDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYG 225
           + AY DAL+ LG DLE VA QE D ALGNGGLGRLASCFLDS+ATLNYPAWGYGLRYKYG
Sbjct: 181 NSAYGDALKRLGFDLESVASQEPDPALGNGGLGRLASCFLDSMATLNYPAWGYGLRYKYG 240

Query: 226 LFKQLITKDGQEEVAENWLEMGNPWEIARNDVFYPVKFYGEVISGADGSKQWVGGEDVTA 285
           LFKQ ITKDGQEE AE+WLE+ NPWEI RNDV YP+KFYG+V+ G+DG K+W+GGED+ A
Sbjct: 241 LFKQRITKDGQEEAAEDWLELSNPWEIVRNDVSYPIKFYGKVVFGSDGKKRWIGGEDIVA 300

Query: 286 VAYDVPIPGYKTKNTINLRLWSTKVAPEQFNLSYFNVGDHASAYAAIKKAEKICYILYPG 345
           VAYDVPIPGYKTK TINLRLWSTK   E F+LS +N G H  A  A+  AEKIC++LYPG
Sbjct: 301 VAYDVPIPGYKTKTTINLRLWSTKAPSEDFDLSSYNSGKHTEAAEALFNAEKICFVLYPG 360

Query: 346 DESLEGKTLRLKQQYTLCSASLQDIVARFERRSGEPVDWQNFPEKVAVQMNDTHPTLCIP 405
           DES EGK LRLKQQYTLCSASLQDIVARFE RSG  V+W+ FPEKVAVQMNDTHPTLCIP
Sbjct: 361 DESTEGKALRLKQQYTLCSASLQDIVARFETRSGGNVNWEEFPEKVAVQMNDTHPTLCIP 420

Query: 406 ELIRILMDVKGLSWKEAWDITRRTVAYTNHTVLPEALEKWSFPLMQELLPRHVEIIEMID 465
           EL+RILMD+KGLSW++AW IT+RTVAYTNHTVLPEALEKWS  LM++LLPRHVEIIE ID
Sbjct: 421 ELMRILMDLKGLSWEDAWKITQRTVAYTNHTVLPEALEKWSLELMEKLLPRHVEIIEKID 480

Query: 466 EELIHSIVAQYGTKDLELLKQKLKQMRILENFELPDSVMEMLVKSTEE--SAIDLVEEAE 525
           EEL+ +IV++YGT D +LL++KLK MRILEN ELP +  +++VK   +  +A D     +
Sbjct: 481 EELVRTIVSEYGTADPDLLEEKLKAMRILENVELPSAFADVIVKPVNKPVTAKDAQNGVK 540

Query: 526 NVDDELLPSEEGDESEDKNIEKKTEVSFGVDPKHPRLIRMANLSVVGGYAVNGVAEIHSE 585
              +E      G+E ED+ I + T     V+P  P+++RMANL+VVGG+AVNGVAEIHSE
Sbjct: 541 TEQEE--EKTAGEEEEDEVIPEPT-----VEP--PKMVRMANLAVVGGHAVNGVAEIHSE 600

Query: 586 IVRTEVFSDFYELWPEKFQNKTNGVTPRRWIRFCNPDLSTIITKWTGTEQWVTDTEKLAI 645
           IV+ +VF+DF +LWPEKFQNKTNGVTPRRWIRFCNP LS IIT W GTE WV +TEK+A 
Sbjct: 601 IVKQDVFNDFVQLWPEKFQNKTNGVTPRRWIRFCNPYLSDIITNWIGTEDWVLNTEKVAE 660

Query: 646 LRKFADNEDLQSMWKEAKRINKLKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNI 705
           LRKFADNEDLQS W+ AK+ NKLKVVS +KE+TGY VSPDAMFD+Q+KRIHEYKRQLLNI
Sbjct: 661 LRKFADNEDLQSEWRAAKKKNKLKVVSLIKERTGYTVSPDAMFDIQIKRIHEYKRQLLNI 720

Query: 706 MGIVYRYKQMKEMNIEEGKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNNDPDI 765
           +GIVYRYK+MKEM+  E +  FVPRVCIFGGKAFATYVQAKRIVKFITDV +T+N+DP+I
Sbjct: 721 LGIVYRYKKMKEMSASEREKAFVPRVCIFGGKAFATYVQAKRIVKFITDVASTINHDPEI 780

Query: 766 GDLLKVVFVPDYNVSVAEVLIPGSDLSQHISTAGMEASGTSNMKFAMNGCILIGTLDGAN 825
           GDLLKV+FVPDYNVSVAE+LIP S+LSQHISTAGMEASGTSNMKF+MNGC+LIGTLDGAN
Sbjct: 781 GDLLKVIFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFSMNGCVLIGTLDGAN 840

Query: 826 VEIREEVGEDNFFLFGARAHEIAGLRKERSQGKFVPDPRFEEVKAFVRSGVFGPYNYEEL 885
           VEIREEVGE+NFFLFGA+A +I  LRKER++GKFVPDP FEEVK FV SGVFG  +Y+EL
Sbjct: 841 VEIREEVGEENFFLFGAKADQIVNLRKERAEGKFVPDPTFEEVKKFVGSGVFGSNSYDEL 900

Query: 886 IGSLEGNEGYGRADYFLVGKDFPSYIECQERVDEAYRDQKRWTKMSILNTAGSYKFSSDR 945
           IGSLEGNEG+GRADYFLVGKDFPSYIECQE+VDEAYRDQKRWT+MSI+NTAGS+KFSSDR
Sbjct: 901 IGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEAYRDQKRWTRMSIMNTAGSFKFSSDR 960

Query: 946 TIHEYARDIWKISPLVL 961
           TIHEYA+DIW I  + L
Sbjct: 961 TIHEYAKDIWNIKQVEL 961

BLAST of MC09g1625 vs. TAIR 10
Match: AT3G46970.1 (alpha-glucan phosphorylase 2 )

HSP 1 Score: 1050.4 bits (2715), Expect = 8.6e-307
Identity = 516/875 (58.97%), Postives = 644/875 (73.60%), Query Frame = 0

Query: 84  DSASVAASIKYHSEFTPSFSPEGFELSKAFYATAESVRDMLIINWNATYEYYEKMNVKQA 143
           D+  +A +I YH++++P FSP  F   +A YATAES+RD LI  WN TY ++ K++ KQ 
Sbjct: 26  DATEIAGNIVYHAKYSPHFSPLKFGPEQALYATAESLRDRLIQLWNETYVHFNKVDPKQT 85

Query: 144 YYLSMEFLQGRALLNAIGNLELSGAYADALRMLGCDLEDVARQESDAALGNGGLGRLASC 203
           YYLSME+LQGRAL NAIGNL L G YADALR LG +LE++A QE DAALGNGGLGRLASC
Sbjct: 86  YYLSMEYLQGRALTNAIGNLNLQGPYADALRTLGYELEEIAEQEKDAALGNGGLGRLASC 145

Query: 204 FLDSLATLNYPAWGYGLRYKYGLFKQLITKDGQEEVAENWLEMGNPWEIARNDVFYPVKF 263
           FLDS+ATLN PAWGYGLRY++GLFKQ+ITK GQEE+ E+WLE  +PWEI R+DV +PV+F
Sbjct: 146 FLDSMATLNLPAWGYGLRYRHGLFKQIITKKGQEEIPEDWLEKFSPWEIVRHDVVFPVRF 205

Query: 264 YGEVISGADGSKQWVGGEDVTAVAYDVPIPGYKTKNTINLRLWSTKVAPEQFNLSYFNVG 323
           +G+V    DGS++WV G+ V A+AYDVPIPGY TKNTI+LRLW  K   E  +L  FN G
Sbjct: 206 FGKVQVNPDGSRKWVDGDVVQALAYDVPIPGYGTKNTISLRLWEAKARAEDLDLFQFNEG 265

Query: 324 DHASAYAAIKKAEKICYILYPGDESLEGKTLRLKQQYTLCSASLQDIVARFERRSGEPVD 383
           ++  A     +A++IC +LYPGD +  GK LRLKQQ+ LCSASLQDI++RF  RS     
Sbjct: 266 EYELAAQLHSRAQQICTVLYPGDATENGKLLRLKQQFFLCSASLQDIISRFHERSTTEGS 325

Query: 384 --WQNFPEKVAVQMNDTHPTLCIPELIRILMDVKGLSWKEAWDITRRTVAYTNHTVLPEA 443
             W  FP KVAVQMNDTHPTL IPEL+R+LMD  GL W EAWD+T +TVAYTNHTVLPEA
Sbjct: 326 RKWSEFPSKVAVQMNDTHPTLAIPELMRLLMDDNGLGWDEAWDVTSKTVAYTNHTVLPEA 385

Query: 444 LEKWSFPLMQELLPRHVEIIEMIDEELIHSIVAQYGTKDLEL-LKQKLKQMRILENFELP 503
           LEKWS  LM +LLPRH+EIIE ID+  + +I      +D  + L+ K+  + IL+N    
Sbjct: 386 LEKWSQSLMWKLLPRHMEIIEEIDKRFVQTI------RDTRVDLEDKISSLSILDN---- 445

Query: 504 DSVMEMLVKSTEESAIDLVEEAENVDDELLPSEEGDESEDKNIEKKTEVSFGVDPKHPRL 563
                                                                +P+ P +
Sbjct: 446 -----------------------------------------------------NPQKP-V 505

Query: 564 IRMANLSVVGGYAVNGVAEIHSEIVRTEVFSDFYELWPEKFQNKTNGVTPRRWIRFCNPD 623
           +RMANL VV  + VNGVA++HS+I++ E+F+D+  +WP KFQNKTNG+TPRRW+RFC+P+
Sbjct: 506 VRMANLCVVSSHTVNGVAQLHSDILKAELFADYVSIWPNKFQNKTNGITPRRWLRFCSPE 565

Query: 624 LSTIITKWTGTEQWVTDTEKLAILRKFADNEDLQSMWKEAKRINKLKVVSFLKEKTGYLV 683
           LS IITKW  T++W+TD + L  LR+FADNE+LQS W  AK  NK ++  +++  TG  +
Sbjct: 566 LSDIITKWLKTDKWITDLDLLTGLRQFADNEELQSEWASAKTANKKRLAQYIERVTGVSI 625

Query: 684 SPDAMFDVQVKRIHEYKRQLLNIMGIVYRYKQMKEMNIEEGKAKFVPRVCIFGGKAFATY 743
            P ++FD+QVKRIHEYKRQL+NI+G+VYR+K++KEM  EE K K VPR  + GGKAFATY
Sbjct: 626 DPTSLFDIQVKRIHEYKRQLMNILGVVYRFKKLKEMKPEERK-KTVPRTVMIGGKAFATY 685

Query: 744 VQAKRIVKFITDVGATVNNDPDIGDLLKVVFVPDYNVSVAEVLIPGSDLSQHISTAGMEA 803
             AKRIVK + DVG  VN+DP++ + LKVVFVP+YNV+VAE+LIPGS+LSQHISTAGMEA
Sbjct: 686 TNAKRIVKLVNDVGDVVNSDPEVNEYLKVVFVPNYNVTVAEMLIPGSELSQHISTAGMEA 745

Query: 804 SGTSNMKFAMNGCILIGTLDGANVEIREEVGEDNFFLFGARAHEIAGLRKERSQGKFVPD 863
           SGTSNMKFA+NGC++IGTLDGANVEIREEVGE+NFFLFGA A ++  LRKER  G F PD
Sbjct: 746 SGTSNMKFALNGCLIIGTLDGANVEIREEVGEENFFLFGATADQVPRLRKEREDGLFKPD 805

Query: 864 PRFEEVKAFVRSGVFGPYNYEELIGSLEGNEGYGRADYFLVGKDFPSYIECQERVDEAYR 923
           PRFEE K FV+SGVFG Y+Y  L+ SLEGN G+GR DYFLVG DFPSY++ Q +VDEAY+
Sbjct: 806 PRFEEAKQFVKSGVFGSYDYGPLLDSLEGNTGFGRGDYFLVGYDFPSYMDAQAKVDEAYK 835

Query: 924 DQKRWTKMSILNTAGSYKFSSDRTIHEYARDIWKI 956
           D+K W KMSIL+TAGS KFSSDRTI +YA++IW I
Sbjct: 866 DRKGWLKMSILSTAGSGKFSSDRTIAQYAKEIWNI 835

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P535350.0e+0075.47Alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic OS=Solanu... [more]
P275980.0e+0073.81Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic OS=Ipomoea ... [more]
P535360.0e+0074.25Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic OS=Vicia fa... [more]
P040450.0e+0072.90Alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic OS=Solanu... [more]
Q9LIB20.0e+0072.96Alpha-glucan phosphorylase 1 OS=Arabidopsis thaliana OX=3702 GN=PHS1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022149091.10.0100.00alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic-like isof... [more]
XP_022149090.10.099.17alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic-like isof... [more]
KAG6574049.10.090.12Alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic, partial ... [more]
XP_022945944.10.090.12alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic-like isof... [more]
XP_022968480.10.090.22alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic-like isof... [more]
Match NameE-valueIdentityDescription
A0A6J1D7B60.0100.00Alpha-1,4 glucan phosphorylase OS=Momordica charantia OX=3673 GN=LOC111017587 PE... [more]
A0A6J1D4S60.099.17Alpha-1,4 glucan phosphorylase OS=Momordica charantia OX=3673 GN=LOC111017587 PE... [more]
A0A6J1G2D10.090.12Alpha-1,4 glucan phosphorylase OS=Cucurbita moschata OX=3662 GN=LOC111450034 PE=... [more]
A0A6J1HXA20.090.22Alpha-1,4 glucan phosphorylase OS=Cucurbita maxima OX=3661 GN=LOC111467708 PE=3 ... [more]
A0A6J1G2F10.089.84Alpha-1,4 glucan phosphorylase OS=Cucurbita moschata OX=3662 GN=LOC111450034 PE=... [more]
Match NameE-valueIdentityDescription
AT3G29320.10.0e+0072.96Glycosyl transferase, family 35 [more]
AT3G46970.18.6e-30758.97alpha-glucan phosphorylase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 508..528
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 611..940
e-value: 3.0E-135
score: 452.9
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 78..610
e-value: 5.5E-211
score: 703.3
NoneNo IPR availablePANTHERPTHR11468:SF27ALPHA-1,4 GLUCAN PHOSPHORYLASE L-2 ISOZYME, CHLOROPLASTIC/AMYLOPLASTICcoord: 28..960
NoneNo IPR availableCDDcd04300GT35_Glycogen_Phosphorylasecoord: 91..955
e-value: 0.0
score: 1220.07
NoneNo IPR availableSUPERFAMILY53756UDP-Glycosyltransferase/glycogen phosphorylasecoord: 84..958
IPR000811Glycosyl transferase, family 35PFAMPF00343Phosphorylasecoord: 174..496
e-value: 4.9E-129
score: 431.7
coord: 545..955
e-value: 2.3E-166
score: 555.1
IPR000811Glycosyl transferase, family 35PIRSFPIRSF000460Glucan_phosphorylase_GlgPcoord: 100..961
e-value: 1.5E-203
score: 676.0
IPR000811Glycosyl transferase, family 35PANTHERPTHR11468GLYCOGEN PHOSPHORYLASEcoord: 28..960
IPR011833Glycogen/starch/alpha-glucan phosphorylaseTIGRFAMTIGR02093TIGR02093coord: 539..955
e-value: 7.9E-167
score: 554.7
IPR035090Phosphorylase pyridoxal-phosphate attachment sitePROSITEPS00102PHOSPHORYLASEcoord: 799..811

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g1625.1MC09g1625.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0005980 glycogen catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0008184 glycogen phosphorylase activity
molecular_function GO:0102250 linear malto-oligosaccharide phosphorylase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0102499 SHG alpha-glucan phosphorylase activity
molecular_function GO:0004645 1,4-alpha-oligoglucan phosphorylase activity