MC09g1452 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC09g1452
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionLipoyl(octanoyl) transferase
LocationMC09: 20505115 .. 20507250 (+)
RNA-Seq ExpressionMC09g1452
SyntenyMC09g1452
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCGCTCCAGTTGCCAAAAACTGAATCCTCTGCAAGAAAACTAAATCCAAATTCAACCTTCTTCTTCTTCTTCTGTTTCTGTTTCTTTTTTCTTTTGTTCATTTCTCTGGTTCGAGTTTTATCTCCGATGGAATGGTTCTTCATTTTACCAATCTTTCTCTAGAATTAAGAGGGCCAGTTCGCCATGATTCTCGTGTCAACGCCCTCTGCCGGCTTGCTTCCATGTCGGCCGGAATTTCAGCGGTCTCGTCCTCGTAAGGTCTGCTCTCAATTGGATCGAATTCTTCACCCAAAAGCTTCCGAATCACCCATAACGCTCAATTCGCGACGCAGAACGTGAGTTTCTCCTTCACGCATTTTCACAAACAGGTTCTGTGCACTTTGTGTGGAATCAACCTATGTGTCTGTCTGGCTTGAGATTTTGCAGGTGCGAGTGCTTCGATTTGCATAATGATCTGATACCGTATGGCGATGCGTGGACTTGGCAAAAGGACATTGTCAGAGAAAAGAAGGCATTGATCGACGCGAACGAGGAATGCCCAGATACGCTTATTGTGTTGCAGCATCATCCTGTGTACACCATGGGCACGGACAGTTCAGAAGAATACCTCAATTTTGATCCAAAGGATTCTCCCTTTTCCATTTACCGCACTGAGCGTGGCGGGGAGGTTACCTATCATGGCCCTGGCCAGGTACTCGCTATGATTTTTTTTGGCCTTTTTCTATCATTTTGCACTACAAATTTTTTATGTTCTCTTCACATTGTAATCTGATGCTTCTTTTCCCCATGTTATGGTAATTTAGGAGTTGAATCTTTGATTTGCTGGTTTGGTTGTTTTAGAGGGTCATTTACTGTTTGCTGTGGTCTCTACTTGATGAAATTGATAGGATTTGTCCTGTGCTGTAGCTAGTTATGTACCCTATTATCAATCTGCGAAATCACAAGATGGACCTTCATTGGTACCTCAGGGCACTTGAGGAGGTGGTTATACGTGTTCTTTCCTCGACGTTTTCCATCAACGCTCATCGGCTCGATGGTTTAACGGGTGTCTGGGCAGGTATTCCTTGTGGGTCTTGGATGAAATTTTTATGTAGCGTATTGCAAATGCACATCTTTTGTCCACTTGCTGATCTGATTTTGATTTCTTCTCATGATTTTGATTGTAAGAGTTATTACCCTGTACTGATAATTTCTAAAGGGCTTTTAAGTGCTTAGTTTCAAACATTATAGATTGTTTCACTTGTTGTTTAACTGGAAATGGAGGCGGTTTTGTTACAAAGTCATAAGAATTTTAGTTCGCTTGGAAATTTTATCAGTACCAACTCGACGAACCAGTCTTATGTTAATAGCTTCAGTAGTCCCTAGTTGGACATTTTTCTTTTTATTTCTAAGAACACTGCCAGCTTCTTGAATGATTTTCCTCTCTTTTCCCTTTGCAGGAAACCAGAAACTGGCAGCCATTGGAATTAGGGTATCTAAATGGATAGCATTTCATGGATTAGCTCTTAATGTCACCACTGATCTAACTCCATTTAACTGGATAGTTCCATGTGGAATACGAAACCGCCAGGTCGGAAACGTCAAGGAGTTGCTTAGAGAAGTTGATTTACACACTGGATTTCAGAAGAACATACATCTTGACAAGGATTTCCAGTTGATTGATACCACTTTTGAAGCTCTGCTACAGGAATTCTCTGAAGTTTTTCAACTTGATATTCATCAGAAAACCATCCCCACTTTGGAAAGGTTCGTGAATCAAGCACCCAAAAGCTGAGGATCCTGTGGTGAGATATCTTATAATCTGTAAACTATAGCTGCTAAACGTTCCTCATTTGTTAGTTTATTTTACTAATATTAGGCACCAACACCCAGCTTTTTCGATCTTAAATCTGCAAACTACTGATTATTTTCCATCTTCTGGGTCTGAAAGGTTCATAATTTAAAGGTTAAAAAACCCTTCCAAACACTTTTGGTCAATGTAATTTGGTTAAAGGCTTTAACCAATTTAGTCCATATACTTTAGTTTGTAACGATTTAGTCTATGTACTCTCAAATGGGTAACAATTTAGTCTTTAAACTTTAATATGTAACAATTTAGTCCTTGTATTTTAAAGTTGATAACGATTTAGTCCTT

mRNA sequence

CTCCGCTCCAGTTGCCAAAAACTGAATCCTCTGCAAGAAAACTAAATCCAAATTCAACCTTCTTCTTCTTCTTCTGTTTCTGTTTCTTTTTTCTTTTGTTCATTTCTCTGGTTCGAGTTTTATCTCCGATGGAATGGTTCTTCATTTTACCAATCTTTCTCTAGAATTAAGAGGGCCAGTTCGCCATGATTCTCGTGTCAACGCCCTCTGCCGGCTTGCTTCCATGTCGGCCGGAATTTCAGCGGTCTCGTCCTCGTAAGGTCTGCTCTCAATTGGATCGAATTCTTCACCCAAAAGCTTCCGAATCACCCATAACGCTCAATTCGCGACGCAGAACGTGCGAGTGCTTCGATTTGCATAATGATCTGATACCGTATGGCGATGCGTGGACTTGGCAAAAGGACATTGTCAGAGAAAAGAAGGCATTGATCGACGCGAACGAGGAATGCCCAGATACGCTTATTGTGTTGCAGCATCATCCTGTGTACACCATGGGCACGGACAGTTCAGAAGAATACCTCAATTTTGATCCAAAGGATTCTCCCTTTTCCATTTACCGCACTGAGCGTGGCGGGGAGGTTACCTATCATGGCCCTGGCCAGCTAGTTATGTACCCTATTATCAATCTGCGAAATCACAAGATGGACCTTCATTGGTACCTCAGGGCACTTGAGGAGGTGGTTATACGTGTTCTTTCCTCGACGTTTTCCATCAACGCTCATCGGCTCGATGGTTTAACGGGTGTCTGGGCAGGAAACCAGAAACTGGCAGCCATTGGAATTAGGGTATCTAAATGGATAGCATTTCATGGATTAGCTCTTAATGTCACCACTGATCTAACTCCATTTAACTGGATAGTTCCATGTGGAATACGAAACCGCCAGGTCGGAAACGTCAAGGAGTTGCTTAGAGAAGTTGATTTACACACTGGATTTCAGAAGAACATACATCTTGACAAGGATTTCCAGTTGATTGATACCACTTTTGAAGCTCTGCTACAGGAATTCTCTGAAGTTTTTCAACTTGATATTCATCAGAAAACCATCCCCACTTTGGAAAGGTTCGTGAATCAAGCACCCAAAAGCTGAGGATCCTGTGGTGAGATATCTTATAATCTGTAAACTATAGCTGCTAAACGTTCCTCATTTGTTAGTTTATTTTACTAATATTAGGCACCAACACCCAGCTTTTTCGATCTTAAATCTGCAAACTACTGATTATTTTCCATCTTCTGGGTCTGAAAGGTTCATAATTTAAAGGTTAAAAAACCCTTCCAAACACTTTTGGTCAATGTAATTTGGTTAAAGGCTTTAACCAATTTAGTCCATATACTTTAGTTTGTAACGATTTAGTCTATGTACTCTCAAATGGGTAACAATTTAGTCTTTAAACTTTAATATGTAACAATTTAGTCCTTGTATTTTAAAGTTGATAACGATTTAGTCCTT

Coding sequence (CDS)

ATGATTCTCGTGTCAACGCCCTCTGCCGGCTTGCTTCCATGTCGGCCGGAATTTCAGCGGTCTCGTCCTCGTAAGGTCTGCTCTCAATTGGATCGAATTCTTCACCCAAAAGCTTCCGAATCACCCATAACGCTCAATTCGCGACGCAGAACGTGCGAGTGCTTCGATTTGCATAATGATCTGATACCGTATGGCGATGCGTGGACTTGGCAAAAGGACATTGTCAGAGAAAAGAAGGCATTGATCGACGCGAACGAGGAATGCCCAGATACGCTTATTGTGTTGCAGCATCATCCTGTGTACACCATGGGCACGGACAGTTCAGAAGAATACCTCAATTTTGATCCAAAGGATTCTCCCTTTTCCATTTACCGCACTGAGCGTGGCGGGGAGGTTACCTATCATGGCCCTGGCCAGCTAGTTATGTACCCTATTATCAATCTGCGAAATCACAAGATGGACCTTCATTGGTACCTCAGGGCACTTGAGGAGGTGGTTATACGTGTTCTTTCCTCGACGTTTTCCATCAACGCTCATCGGCTCGATGGTTTAACGGGTGTCTGGGCAGGAAACCAGAAACTGGCAGCCATTGGAATTAGGGTATCTAAATGGATAGCATTTCATGGATTAGCTCTTAATGTCACCACTGATCTAACTCCATTTAACTGGATAGTTCCATGTGGAATACGAAACCGCCAGGTCGGAAACGTCAAGGAGTTGCTTAGAGAAGTTGATTTACACACTGGATTTCAGAAGAACATACATCTTGACAAGGATTTCCAGTTGATTGATACCACTTTTGAAGCTCTGCTACAGGAATTCTCTGAAGTTTTTCAACTTGATATTCATCAGAAAACCATCCCCACTTTGGAAAGGTTCGTGAATCAAGCACCCAAAAGCTGA

Protein sequence

MILVSTPSAGLLPCRPEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCECFDLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNFDPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFSINAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQVGNVKELLREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPTLERFVNQAPKS
Homology
BLAST of MC09g1452 vs. ExPASy Swiss-Prot
Match: Q948J9 (Octanoyltransferase LIP2p, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LIP2P PE=1 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 4.7e-99
Identity = 169/238 (71.01%), Postives = 198/238 (83.19%), Query Frame = 0

Query: 50  RTCECFDLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSE 109
           R C CFDL++ L+PY  AW+WQK IV EKK LID N++C DT+I+LQH PVYTMGT S+E
Sbjct: 43  RKCLCFDLYDKLVPYKKAWSWQKSIVEEKKTLIDRNQDCADTVILLQHSPVYTMGTASTE 102

Query: 110 EYLNFDPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRV 169
           +YLNFD KD+PF++YRTERGGEVTYHGPGQLVMYPIINLRNH+MDLHWYLR LEE+VIRV
Sbjct: 103 DYLNFDIKDAPFNVYRTERGGEVTYHGPGQLVMYPIINLRNHEMDLHWYLRMLEEIVIRV 162

Query: 170 LSSTFSINAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGI 229
           LSSTFSI A RLDGLTGVW GNQK+AAIGIRVSKWI +HGLALNVTTDLTPFNWIVPCGI
Sbjct: 163 LSSTFSIKASRLDGLTGVWVGNQKVAAIGIRVSKWITYHGLALNVTTDLTPFNWIVPCGI 222

Query: 230 RNRQVGNVKELLREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTI 288
           R+R+VGN+K LL + +          +  D +LID   E+LL+EFSE FQL I ++T+
Sbjct: 223 RDRKVGNIKGLLEDGE--------HGMVDDLRLIDIVHESLLKEFSEAFQLQIEKQTV 272

BLAST of MC09g1452 vs. ExPASy Swiss-Prot
Match: P0C7R2 (Octanoyltransferase LIP2p2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LIP2P2 PE=1 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 1.8e-98
Identity = 174/259 (67.18%), Postives = 205/259 (79.15%), Query Frame = 0

Query: 37  KASESPITLNSRRRT----CECFDLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTL 96
           + S S   +NS+  T    CECFDL++ LIPY  AW+WQK I+ EKKALID N+EC D+L
Sbjct: 28  RVSTSLKIMNSKNHTNPRKCECFDLYDQLIPYKKAWSWQKSILNEKKALIDKNQECSDSL 87

Query: 97  IVLQHHPVYTMGTDSSEEYLNFDPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHK 156
           I+LQH  VYTMGT SSE YLNFD K++PF +YRTERGGEVTYHGPGQLVMYPIINLRNHK
Sbjct: 88  IILQHPSVYTMGTGSSENYLNFDIKNAPFDVYRTERGGEVTYHGPGQLVMYPIINLRNHK 147

Query: 157 MDLHWYLRALEEVVIRVLSSTFSINAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLAL 216
           MDLHWYLR LEEVVIRVLSS F+INA RLDG TGVW GN+K+AAIGIRVSKW+ +HGLAL
Sbjct: 148 MDLHWYLRKLEEVVIRVLSSAFAINASRLDGFTGVWVGNKKMAAIGIRVSKWMTYHGLAL 207

Query: 217 NVTTDLTPFNWIVPCGIRNRQVGNVKELLREVDLHTGFQKNIHLDKDFQLIDTTFEALLQ 276
           NVTTDLTPFN IVPCGIRNR VG+VK L+ + + +   +       D QL+D   E+LL+
Sbjct: 208 NVTTDLTPFNSIVPCGIRNRGVGSVKGLIEDGEHYNKLE-------DLQLLDIAHESLLK 267

Query: 277 EFSEVFQLDIHQKTIPTLE 292
           EFSEVFQL + ++T+  LE
Sbjct: 268 EFSEVFQLQMEKQTVFKLE 279

BLAST of MC09g1452 vs. ExPASy Swiss-Prot
Match: Q3M698 (Octanoyltransferase OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=lipB PE=3 SV=2)

HSP 1 Score: 208.0 bits (528), Expect = 1.5e-52
Identity = 100/186 (53.76%), Postives = 129/186 (69.35%), Query Frame = 0

Query: 54  CFDLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLN 113
           C   +  L+PY +A  WQ+ ++ ++   ID      D LI+L+H PVYT+G  SS E+L 
Sbjct: 12  CLLYNKGLMPYLEAHAWQRSLLHQR---ID-YPNLEDVLILLEHPPVYTLGQGSSLEFLK 71

Query: 114 FDPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSST 173
           FDP  S F I+R ERGGEVTYH PGQLV YPI+NLR H+ DLHWYLR LEEV+IRVL + 
Sbjct: 72  FDPNQSEFEIHRIERGGEVTYHCPGQLVGYPILNLRRHRQDLHWYLRQLEEVIIRVL-AV 131

Query: 174 FSINAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQ 233
           + +   RL  LTGVW   +K+AAIGI+VS+WI  HG ALNV  D+T F  IVPCGI ++ 
Sbjct: 132 YGLKGDRLPSLTGVWLEGRKVAAIGIKVSRWITMHGFALNVCPDMTGFGRIVPCGITDKP 191

Query: 234 VGNVKE 240
           VG++ +
Sbjct: 192 VGSLAQ 192

BLAST of MC09g1452 vs. ExPASy Swiss-Prot
Match: Q8YSA4 (Octanoyltransferase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=lipB PE=3 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 3.3e-52
Identity = 100/186 (53.76%), Postives = 129/186 (69.35%), Query Frame = 0

Query: 54  CFDLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLN 113
           C   +  L+PY  A  WQ+ ++R++   ID      D LI+L+H PVYT+G  SS E+L 
Sbjct: 12  CLLYNKGLMPYLAAHAWQRSLLRQR---ID-YPNLEDVLILLEHPPVYTLGQGSSLEFLK 71

Query: 114 FDPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSST 173
           FDP  S F I+R ERGGEVTYH PGQLV YPI+NL  H+ DLHWYLR LEEV+IRVL + 
Sbjct: 72  FDPNQSEFEIHRIERGGEVTYHCPGQLVGYPILNLHRHRQDLHWYLRQLEEVIIRVL-AV 131

Query: 174 FSINAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQ 233
           + +   RL  LTGVW   +K+AAIGI+VS+WI  HG ALNV  D+T F+ IVPCGI ++ 
Sbjct: 132 YDLKGDRLPSLTGVWLEGRKVAAIGIKVSRWITMHGFALNVCPDMTGFSRIVPCGITDKP 191

Query: 234 VGNVKE 240
           VG++ +
Sbjct: 192 VGSLAQ 192

BLAST of MC09g1452 vs. ExPASy Swiss-Prot
Match: Q8DKM7 (Octanoyltransferase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=lipB PE=3 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 5.7e-52
Identity = 93/186 (50.00%), Postives = 127/186 (68.28%), Query Frame = 0

Query: 61  LIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNFDPKDSP 120
           +IPY DAW WQ+ +V E++     N   PD L+ L+H  +YT+G  ++  +++FDP  + 
Sbjct: 13  IIPYRDAWRWQQQLVAERR----QNLAAPDVLLTLEHPAIYTLGQGATTAHIHFDPVATG 72

Query: 121 FSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFSINAHR 180
             + R ERGG+VTYH PGQLV YPI+NLR H  DLHWYL  LEEV+I+ L   + I   R
Sbjct: 73  IEVLRIERGGDVTYHCPGQLVAYPILNLRRHCCDLHWYLHQLEEVIIQTLRH-YGIKGER 132

Query: 181 LDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQVGNVKEL 240
           + GLTGVW    K+AAIGI+V++WI+FHG+ALNV  DL  F  IVPCGI +R VGN+++ 
Sbjct: 133 VAGLTGVWVEGYKVAAIGIKVTRWISFHGIALNVVNDLKGFGRIVPCGIGDRPVGNLRQF 192

Query: 241 LREVDL 247
             ++ L
Sbjct: 193 CADIQL 193

BLAST of MC09g1452 vs. NCBI nr
Match: XP_022150592.1 (plastidial lipoyltransferase 2-like [Momordica charantia] >XP_022150593.1 plastidial lipoyltransferase 2-like [Momordica charantia])

HSP 1 Score: 625 bits (1611), Expect = 3.78e-226
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0

Query: 1   MILVSTPSAGLLPCRPEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCECFDLHND 60
           MILVSTPSAGLLPCRPEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCECFDLHND
Sbjct: 1   MILVSTPSAGLLPCRPEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCECFDLHND 60

Query: 61  LIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNFDPKDSP 120
           LIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNFDPKDSP
Sbjct: 61  LIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNFDPKDSP 120

Query: 121 FSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFSINAHR 180
           FSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFSINAHR
Sbjct: 121 FSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFSINAHR 180

Query: 181 LDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQVGNVKEL 240
           LDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQVGNVKEL
Sbjct: 181 LDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQVGNVKEL 240

Query: 241 LREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPTLERFVNQAPKS 300
           LREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPTLERFVNQAPKS
Sbjct: 241 LREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPTLERFVNQAPKS 300

BLAST of MC09g1452 vs. NCBI nr
Match: XP_031742007.1 (octanoyltransferase LIP2p, chloroplastic isoform X3 [Cucumis sativus])

HSP 1 Score: 493 bits (1269), Expect = 5.39e-174
Identity = 239/305 (78.36%), Postives = 266/305 (87.21%), Query Frame = 0

Query: 2   ILVSTPSAGLLPCR------PEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCECF 61
           +++STPSAG LP R      PE QRS P+ +  + D+ILH K S S + LN+R ++CECF
Sbjct: 1   MILSTPSAGFLPSRLSTHRPPESQRSHPQNISYKFDQILHKKPSGSSLPLNARPKSCECF 60

Query: 62  DLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNFD 121
           DLH +LIPYGDAW+WQK+IVREK  LIDAN++CPDTLIVLQHHPVYTMGT+S+EEYLNFD
Sbjct: 61  DLHQNLIPYGDAWSWQKEIVREKVGLIDANQDCPDTLIVLQHHPVYTMGTNSTEEYLNFD 120

Query: 122 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 181
             DSPF IYRTERGGEVTYHGPGQLVMYPI+NLRNHKMDLHWYLRALEEVVIRVLSSTFS
Sbjct: 121 LNDSPFPIYRTERGGEVTYHGPGQLVMYPILNLRNHKMDLHWYLRALEEVVIRVLSSTFS 180

Query: 182 INAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQVG 241
           INAHR+DGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGI+NR+VG
Sbjct: 181 INAHRIDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIQNRKVG 240

Query: 242 NVKELLREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPTLERFVN 300
           N+KELLREVDLHTGFQK      D QLID TFEAL+QEFSEVFQLDI+ KTIP+LE F+ 
Sbjct: 241 NIKELLREVDLHTGFQK------DSQLIDITFEALIQEFSEVFQLDIYHKTIPSLE-FLK 298

BLAST of MC09g1452 vs. NCBI nr
Match: XP_031742006.1 (octanoyltransferase LIP2p, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 493 bits (1269), Expect = 6.01e-174
Identity = 239/305 (78.36%), Postives = 266/305 (87.21%), Query Frame = 0

Query: 2   ILVSTPSAGLLPCR------PEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCECF 61
           +++STPSAG LP R      PE QRS P+ +  + D+ILH K S S + LN+R ++CECF
Sbjct: 1   MILSTPSAGFLPSRLSTHRPPESQRSHPQNISYKFDQILHKKPSGSSLPLNARPKSCECF 60

Query: 62  DLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNFD 121
           DLH +LIPYGDAW+WQK+IVREK  LIDAN++CPDTLIVLQHHPVYTMGT+S+EEYLNFD
Sbjct: 61  DLHQNLIPYGDAWSWQKEIVREKVGLIDANQDCPDTLIVLQHHPVYTMGTNSTEEYLNFD 120

Query: 122 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 181
             DSPF IYRTERGGEVTYHGPGQLVMYPI+NLRNHKMDLHWYLRALEEVVIRVLSSTFS
Sbjct: 121 LNDSPFPIYRTERGGEVTYHGPGQLVMYPILNLRNHKMDLHWYLRALEEVVIRVLSSTFS 180

Query: 182 INAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQVG 241
           INAHR+DGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGI+NR+VG
Sbjct: 181 INAHRIDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIQNRKVG 240

Query: 242 NVKELLREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPTLERFVN 300
           N+KELLREVDLHTGFQK      D QLID TFEAL+QEFSEVFQLDI+ KTIP+LE F+ 
Sbjct: 241 NIKELLREVDLHTGFQK------DSQLIDITFEALIQEFSEVFQLDIYHKTIPSLE-FLK 298

BLAST of MC09g1452 vs. NCBI nr
Match: XP_004135053.2 (octanoyltransferase LIP2p, chloroplastic isoform X1 [Cucumis sativus] >KGN52158.1 hypothetical protein Csa_008925 [Cucumis sativus])

HSP 1 Score: 493 bits (1269), Expect = 1.07e-173
Identity = 239/305 (78.36%), Postives = 266/305 (87.21%), Query Frame = 0

Query: 2   ILVSTPSAGLLPCR------PEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCECF 61
           +++STPSAG LP R      PE QRS P+ +  + D+ILH K S S + LN+R ++CECF
Sbjct: 1   MILSTPSAGFLPSRLSTHRPPESQRSHPQNISYKFDQILHKKPSGSSLPLNARPKSCECF 60

Query: 62  DLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNFD 121
           DLH +LIPYGDAW+WQK+IVREK  LIDAN++CPDTLIVLQHHPVYTMGT+S+EEYLNFD
Sbjct: 61  DLHQNLIPYGDAWSWQKEIVREKVGLIDANQDCPDTLIVLQHHPVYTMGTNSTEEYLNFD 120

Query: 122 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 181
             DSPF IYRTERGGEVTYHGPGQLVMYPI+NLRNHKMDLHWYLRALEEVVIRVLSSTFS
Sbjct: 121 LNDSPFPIYRTERGGEVTYHGPGQLVMYPILNLRNHKMDLHWYLRALEEVVIRVLSSTFS 180

Query: 182 INAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQVG 241
           INAHR+DGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGI+NR+VG
Sbjct: 181 INAHRIDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIQNRKVG 240

Query: 242 NVKELLREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPTLERFVN 300
           N+KELLREVDLHTGFQK      D QLID TFEAL+QEFSEVFQLDI+ KTIP+LE F+ 
Sbjct: 241 NIKELLREVDLHTGFQK------DSQLIDITFEALIQEFSEVFQLDIYHKTIPSLE-FLK 298

BLAST of MC09g1452 vs. NCBI nr
Match: XP_016900264.1 (PREDICTED: plastidial lipoyltransferase 2-like [Cucumis melo])

HSP 1 Score: 483 bits (1242), Expect = 8.36e-170
Identity = 238/306 (77.78%), Postives = 264/306 (86.27%), Query Frame = 0

Query: 1   MILVSTPSAGLLP-CR-----PEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCEC 60
           MIL+ TPSAG LP C      PE QRS P+K+  + D+IL  K S SP+ LN+  ++CEC
Sbjct: 1   MILL-TPSAGFLPSCSNTHRPPESQRSHPQKIFYKFDQILRKKPSGSPLPLNALPKSCEC 60

Query: 61  FDLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNF 120
           FDLH +LIPYGDAW+WQK+IVREK  LIDAN++CPDTLIVLQHHPVYTMGT+S+EEY NF
Sbjct: 61  FDLHQNLIPYGDAWSWQKEIVREKVGLIDANQDCPDTLIVLQHHPVYTMGTNSTEEYFNF 120

Query: 121 DPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTF 180
           D KDSPF IYRTERGGEVTYHGPGQLVMYPI+NLRNHKMDLHWYLRALEEVVIRVLSSTF
Sbjct: 121 DLKDSPFPIYRTERGGEVTYHGPGQLVMYPIVNLRNHKMDLHWYLRALEEVVIRVLSSTF 180

Query: 181 SINAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQV 240
           SINAHR+DGLTGVWAGNQKLAAIGIRVSKWI FHGLALNVTTDLTPFNWIVPCGIRNR+V
Sbjct: 181 SINAHRIDGLTGVWAGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRKV 240

Query: 241 GNVKELLREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPTLERFV 300
           GN+KELLREVDLHTGFQK      D QLID TF AL+QEFSEVFQLD++ KTIP+LE F+
Sbjct: 241 GNIKELLREVDLHTGFQK------DSQLIDITFGALIQEFSEVFQLDMYHKTIPSLE-FL 298

BLAST of MC09g1452 vs. ExPASy TrEMBL
Match: A0A6J1D9U7 (Lipoyl(octanoyl) transferase OS=Momordica charantia OX=3673 GN=LOC111018691 PE=3 SV=1)

HSP 1 Score: 625 bits (1611), Expect = 1.83e-226
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 0

Query: 1   MILVSTPSAGLLPCRPEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCECFDLHND 60
           MILVSTPSAGLLPCRPEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCECFDLHND
Sbjct: 1   MILVSTPSAGLLPCRPEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCECFDLHND 60

Query: 61  LIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNFDPKDSP 120
           LIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNFDPKDSP
Sbjct: 61  LIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNFDPKDSP 120

Query: 121 FSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFSINAHR 180
           FSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFSINAHR
Sbjct: 121 FSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFSINAHR 180

Query: 181 LDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQVGNVKEL 240
           LDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQVGNVKEL
Sbjct: 181 LDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQVGNVKEL 240

Query: 241 LREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPTLERFVNQAPKS 300
           LREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPTLERFVNQAPKS
Sbjct: 241 LREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPTLERFVNQAPKS 300

BLAST of MC09g1452 vs. ExPASy TrEMBL
Match: A0A0A0KRC7 (Lipoyl(octanoyl) transferase OS=Cucumis sativus OX=3659 GN=Csa_5G612870 PE=3 SV=1)

HSP 1 Score: 493 bits (1269), Expect = 5.20e-174
Identity = 239/305 (78.36%), Postives = 266/305 (87.21%), Query Frame = 0

Query: 2   ILVSTPSAGLLPCR------PEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCECF 61
           +++STPSAG LP R      PE QRS P+ +  + D+ILH K S S + LN+R ++CECF
Sbjct: 1   MILSTPSAGFLPSRLSTHRPPESQRSHPQNISYKFDQILHKKPSGSSLPLNARPKSCECF 60

Query: 62  DLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNFD 121
           DLH +LIPYGDAW+WQK+IVREK  LIDAN++CPDTLIVLQHHPVYTMGT+S+EEYLNFD
Sbjct: 61  DLHQNLIPYGDAWSWQKEIVREKVGLIDANQDCPDTLIVLQHHPVYTMGTNSTEEYLNFD 120

Query: 122 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 181
             DSPF IYRTERGGEVTYHGPGQLVMYPI+NLRNHKMDLHWYLRALEEVVIRVLSSTFS
Sbjct: 121 LNDSPFPIYRTERGGEVTYHGPGQLVMYPILNLRNHKMDLHWYLRALEEVVIRVLSSTFS 180

Query: 182 INAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQVG 241
           INAHR+DGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGI+NR+VG
Sbjct: 181 INAHRIDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIQNRKVG 240

Query: 242 NVKELLREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPTLERFVN 300
           N+KELLREVDLHTGFQK      D QLID TFEAL+QEFSEVFQLDI+ KTIP+LE F+ 
Sbjct: 241 NIKELLREVDLHTGFQK------DSQLIDITFEALIQEFSEVFQLDIYHKTIPSLE-FLK 298

BLAST of MC09g1452 vs. ExPASy TrEMBL
Match: A0A1S4DWA5 (Lipoyl(octanoyl) transferase OS=Cucumis melo OX=3656 GN=LOC103489357 PE=3 SV=1)

HSP 1 Score: 483 bits (1242), Expect = 4.05e-170
Identity = 238/306 (77.78%), Postives = 264/306 (86.27%), Query Frame = 0

Query: 1   MILVSTPSAGLLP-CR-----PEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCEC 60
           MIL+ TPSAG LP C      PE QRS P+K+  + D+IL  K S SP+ LN+  ++CEC
Sbjct: 1   MILL-TPSAGFLPSCSNTHRPPESQRSHPQKIFYKFDQILRKKPSGSPLPLNALPKSCEC 60

Query: 61  FDLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNF 120
           FDLH +LIPYGDAW+WQK+IVREK  LIDAN++CPDTLIVLQHHPVYTMGT+S+EEY NF
Sbjct: 61  FDLHQNLIPYGDAWSWQKEIVREKVGLIDANQDCPDTLIVLQHHPVYTMGTNSTEEYFNF 120

Query: 121 DPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTF 180
           D KDSPF IYRTERGGEVTYHGPGQLVMYPI+NLRNHKMDLHWYLRALEEVVIRVLSSTF
Sbjct: 121 DLKDSPFPIYRTERGGEVTYHGPGQLVMYPIVNLRNHKMDLHWYLRALEEVVIRVLSSTF 180

Query: 181 SINAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQV 240
           SINAHR+DGLTGVWAGNQKLAAIGIRVSKWI FHGLALNVTTDLTPFNWIVPCGIRNR+V
Sbjct: 181 SINAHRIDGLTGVWAGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRKV 240

Query: 241 GNVKELLREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPTLERFV 300
           GN+KELLREVDLHTGFQK      D QLID TF AL+QEFSEVFQLD++ KTIP+LE F+
Sbjct: 241 GNIKELLREVDLHTGFQK------DSQLIDITFGALIQEFSEVFQLDMYHKTIPSLE-FL 298

BLAST of MC09g1452 vs. ExPASy TrEMBL
Match: A0A5D3CD13 (Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001740 PE=3 SV=1)

HSP 1 Score: 483 bits (1244), Expect = 1.64e-167
Identity = 235/306 (76.80%), Postives = 264/306 (86.27%), Query Frame = 0

Query: 1   MILVSTPSAGLLP-CR-----PEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCEC 60
           ++++ TPSAG LP C      PE QRS P+K+  + D+IL  K S SP+ LN+  ++CEC
Sbjct: 191 LMILLTPSAGFLPSCSNTHRPPESQRSHPQKIFYKFDQILRKKPSGSPLPLNALPKSCEC 250

Query: 61  FDLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNF 120
           FDLH +LIPYGDAW+WQK+IVREK  LIDAN++CPDTLIVLQHHPVYTMGT+S+EEY NF
Sbjct: 251 FDLHQNLIPYGDAWSWQKEIVREKVGLIDANQDCPDTLIVLQHHPVYTMGTNSTEEYFNF 310

Query: 121 DPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTF 180
           D KDSPF IYRTERGGEVTYHGPGQLVMYPI+NLRNHKMDLHWYLRALEEVVIRVLSSTF
Sbjct: 311 DLKDSPFPIYRTERGGEVTYHGPGQLVMYPIVNLRNHKMDLHWYLRALEEVVIRVLSSTF 370

Query: 181 SINAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQV 240
           SINAHR+DGLTGVWAGNQKLAAIGIRVSKWI FHGLALNVTTDLTPFNWIVPCGIRNR+V
Sbjct: 371 SINAHRIDGLTGVWAGNQKLAAIGIRVSKWITFHGLALNVTTDLTPFNWIVPCGIRNRKV 430

Query: 241 GNVKELLREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPTLERFV 300
           GN+KELLREVDLHTGFQK      D QLID TF AL+QEFSEVFQLD++ KTIP+LE F+
Sbjct: 431 GNIKELLREVDLHTGFQK------DSQLIDITFGALIQEFSEVFQLDMYHKTIPSLE-FL 489

BLAST of MC09g1452 vs. ExPASy TrEMBL
Match: A0A6J1G154 (Lipoyl(octanoyl) transferase OS=Cucurbita moschata OX=3662 GN=LOC111449727 PE=3 SV=1)

HSP 1 Score: 470 bits (1209), Expect = 4.44e-165
Identity = 231/307 (75.24%), Postives = 263/307 (85.67%), Query Frame = 0

Query: 2   ILVSTPSAGLLPC------RPEFQRSRPRKVCSQLDRILHPKASESPITLNSRRRTCECF 61
           +++STPSA   P       RPE   S PRKV ++ DR+LHPKAS+S +TLN+++RTCECF
Sbjct: 3   MILSTPSASFFPSCGRTHRRPESPWSHPRKVIAKFDRVLHPKASKSRLTLNAQQRTCECF 62

Query: 62  DLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSEEYLNFD 121
           DLH DLIPY DAW+WQKDIVREK ALIDANE+CPD+LIVLQH PVYT+GT+S+EE+LNFD
Sbjct: 63  DLHRDLIPYNDAWSWQKDIVREKIALIDANEDCPDSLIVLQHPPVYTLGTNSTEEFLNFD 122

Query: 122 PKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRVLSSTFS 181
           PKDSPFSIYRTERGGEVTYHGPGQ+VMYPIINLRNHKMDLHWYLRALEEV+IRVLSSTFS
Sbjct: 123 PKDSPFSIYRTERGGEVTYHGPGQIVMYPIINLRNHKMDLHWYLRALEEVIIRVLSSTFS 182

Query: 182 INAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGIRNRQVG 241
           INA+R+DGLTGVWAGNQKLAAIGIRVSKWI FHGLALNVTTDL+PFN IVPCGIRNRQVG
Sbjct: 183 INANRVDGLTGVWAGNQKLAAIGIRVSKWITFHGLALNVTTDLSPFNLIVPCGIRNRQVG 242

Query: 242 NVKELLREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTIPT--LERF 300
           N+KELL    L    + +IH DKD QLID TFEAL+ +F++VFQ+DIHQKT P   LE  
Sbjct: 243 NIKELLL---LLREAENSIHFDKDSQLIDITFEALIHQFAQVFQVDIHQKTTPPPFLES- 302

BLAST of MC09g1452 vs. TAIR 10
Match: AT4G31050.1 (Biotin/lipoate A/B protein ligase family )

HSP 1 Score: 362.5 bits (929), Expect = 3.4e-100
Identity = 169/238 (71.01%), Postives = 198/238 (83.19%), Query Frame = 0

Query: 50  RTCECFDLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSE 109
           R C CFDL++ L+PY  AW+WQK IV EKK LID N++C DT+I+LQH PVYTMGT S+E
Sbjct: 43  RKCLCFDLYDKLVPYKKAWSWQKSIVEEKKTLIDRNQDCADTVILLQHSPVYTMGTASTE 102

Query: 110 EYLNFDPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVVIRV 169
           +YLNFD KD+PF++YRTERGGEVTYHGPGQLVMYPIINLRNH+MDLHWYLR LEE+VIRV
Sbjct: 103 DYLNFDIKDAPFNVYRTERGGEVTYHGPGQLVMYPIINLRNHEMDLHWYLRMLEEIVIRV 162

Query: 170 LSSTFSINAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIVPCGI 229
           LSSTFSI A RLDGLTGVW GNQK+AAIGIRVSKWI +HGLALNVTTDLTPFNWIVPCGI
Sbjct: 163 LSSTFSIKASRLDGLTGVWVGNQKVAAIGIRVSKWITYHGLALNVTTDLTPFNWIVPCGI 222

Query: 230 RNRQVGNVKELLREVDLHTGFQKNIHLDKDFQLIDTTFEALLQEFSEVFQLDIHQKTI 288
           R+R+VGN+K LL + +          +  D +LID   E+LL+EFSE FQL I ++T+
Sbjct: 223 RDRKVGNIKGLLEDGE--------HGMVDDLRLIDIVHESLLKEFSEAFQLQIEKQTV 272

BLAST of MC09g1452 vs. TAIR 10
Match: AT1G47578.1 (Biotin/lipoate A/B protein ligase family )

HSP 1 Score: 360.5 bits (924), Expect = 1.3e-99
Identity = 174/259 (67.18%), Postives = 205/259 (79.15%), Query Frame = 0

Query: 37  KASESPITLNSRRRT----CECFDLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTL 96
           + S S   +NS+  T    CECFDL++ LIPY  AW+WQK I+ EKKALID N+EC D+L
Sbjct: 28  RVSTSLKIMNSKNHTNPRKCECFDLYDQLIPYKKAWSWQKSILNEKKALIDKNQECSDSL 87

Query: 97  IVLQHHPVYTMGTDSSEEYLNFDPKDSPFSIYRTERGGEVTYHGPGQLVMYPIINLRNHK 156
           I+LQH  VYTMGT SSE YLNFD K++PF +YRTERGGEVTYHGPGQLVMYPIINLRNHK
Sbjct: 88  IILQHPSVYTMGTGSSENYLNFDIKNAPFDVYRTERGGEVTYHGPGQLVMYPIINLRNHK 147

Query: 157 MDLHWYLRALEEVVIRVLSSTFSINAHRLDGLTGVWAGNQKLAAIGIRVSKWIAFHGLAL 216
           MDLHWYLR LEEVVIRVLSS F+INA RLDG TGVW GN+K+AAIGIRVSKW+ +HGLAL
Sbjct: 148 MDLHWYLRKLEEVVIRVLSSAFAINASRLDGFTGVWVGNKKMAAIGIRVSKWMTYHGLAL 207

Query: 217 NVTTDLTPFNWIVPCGIRNRQVGNVKELLREVDLHTGFQKNIHLDKDFQLIDTTFEALLQ 276
           NVTTDLTPFN IVPCGIRNR VG+VK L+ + + +   +       D QL+D   E+LL+
Sbjct: 208 NVTTDLTPFNSIVPCGIRNRGVGSVKGLIEDGEHYNKLE-------DLQLLDIAHESLLK 267

Query: 277 EFSEVFQLDIHQKTIPTLE 292
           EFSEVFQL + ++T+  LE
Sbjct: 268 EFSEVFQLQMEKQTVFKLE 279

BLAST of MC09g1452 vs. TAIR 10
Match: AT1G04640.1 (lipoyltransferase 2 )

HSP 1 Score: 129.4 bits (324), Expect = 4.8e-30
Identity = 75/210 (35.71%), Postives = 118/210 (56.19%), Query Frame = 0

Query: 50  RTCECFDLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSE 109
           RT E + L    + Y  +   Q+ +V E+KA      + PDTL+ LQH P YT+G   ++
Sbjct: 5   RTLEVWKL--GTVNYLKSLKLQEKLVSERKA-----HQIPDTLLSLQHPPTYTLGKRRTD 64

Query: 110 EYLNFDPKDSP---FSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVV 169
             L     +       ++ T+RGG++T+HGP Q ++YPII+LR+       Y+  LE  +
Sbjct: 65  HNLLIPESELTKIGAELHYTQRGGDITFHGPHQAILYPIISLRSIGFGARNYVETLERSM 124

Query: 170 IRVLSSTFSINAHRLDGL-TGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIV 229
           I   +S + + A   +   TGVW G++K+ AIG+R+S  I  HGLALN+  D+  F  IV
Sbjct: 125 IE-FASIYGVKARAGNKCETGVWVGDRKIGAIGVRISSGITSHGLALNIDPDMKYFEHIV 184

Query: 230 PCGIRNRQVGNVKELLREVDLHTGFQKNIH 256
           PCGI +++V +++   RE D     ++ IH
Sbjct: 185 PCGIADKEVTSLR---RETDTLLPSEEVIH 203

BLAST of MC09g1452 vs. TAIR 10
Match: AT1G04640.2 (lipoyltransferase 2 )

HSP 1 Score: 129.4 bits (324), Expect = 4.8e-30
Identity = 75/210 (35.71%), Postives = 118/210 (56.19%), Query Frame = 0

Query: 50  RTCECFDLHNDLIPYGDAWTWQKDIVREKKALIDANEECPDTLIVLQHHPVYTMGTDSSE 109
           RT E + L    + Y  +   Q+ +V E+KA      + PDTL+ LQH P YT+G   ++
Sbjct: 5   RTLEVWKL--GTVNYLKSLKLQEKLVSERKA-----HQIPDTLLSLQHPPTYTLGKRRTD 64

Query: 110 EYLNFDPKDSP---FSIYRTERGGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRALEEVV 169
             L     +       ++ T+RGG++T+HGP Q ++YPII+LR+       Y+  LE  +
Sbjct: 65  HNLLIPESELTKIGAELHYTQRGGDITFHGPHQAILYPIISLRSIGFGARNYVETLERSM 124

Query: 170 IRVLSSTFSINAHRLDGL-TGVWAGNQKLAAIGIRVSKWIAFHGLALNVTTDLTPFNWIV 229
           I   +S + + A   +   TGVW G++K+ AIG+R+S  I  HGLALN+  D+  F  IV
Sbjct: 125 IE-FASIYGVKARAGNKCETGVWVGDRKIGAIGVRISSGITSHGLALNIDPDMKYFEHIV 184

Query: 230 PCGIRNRQVGNVKELLREVDLHTGFQKNIH 256
           PCGI +++V +++   RE D     ++ IH
Sbjct: 185 PCGIADKEVTSLR---RETDTLLPSEEVIH 203

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q948J94.7e-9971.01Octanoyltransferase LIP2p, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LIP2... [more]
P0C7R21.8e-9867.18Octanoyltransferase LIP2p2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LIP... [more]
Q3M6981.5e-5253.76Octanoyltransferase OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=2... [more]
Q8YSA43.3e-5253.76Octanoyltransferase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=1... [more]
Q8DKM75.7e-5250.00Octanoyltransferase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=... [more]
Match NameE-valueIdentityDescription
XP_022150592.13.78e-226100.00plastidial lipoyltransferase 2-like [Momordica charantia] >XP_022150593.1 plasti... [more]
XP_031742007.15.39e-17478.36octanoyltransferase LIP2p, chloroplastic isoform X3 [Cucumis sativus][more]
XP_031742006.16.01e-17478.36octanoyltransferase LIP2p, chloroplastic isoform X2 [Cucumis sativus][more]
XP_004135053.21.07e-17378.36octanoyltransferase LIP2p, chloroplastic isoform X1 [Cucumis sativus] >KGN52158.... [more]
XP_016900264.18.36e-17077.78PREDICTED: plastidial lipoyltransferase 2-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1D9U71.83e-226100.00Lipoyl(octanoyl) transferase OS=Momordica charantia OX=3673 GN=LOC111018691 PE=3... [more]
A0A0A0KRC75.20e-17478.36Lipoyl(octanoyl) transferase OS=Cucumis sativus OX=3659 GN=Csa_5G612870 PE=3 SV=... [more]
A0A1S4DWA54.05e-17077.78Lipoyl(octanoyl) transferase OS=Cucumis melo OX=3656 GN=LOC103489357 PE=3 SV=1[more]
A0A5D3CD131.64e-16776.80Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A6J1G1544.44e-16575.24Lipoyl(octanoyl) transferase OS=Cucurbita moschata OX=3662 GN=LOC111449727 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT4G31050.13.4e-10071.01Biotin/lipoate A/B protein ligase family [more]
AT1G47578.11.3e-9967.18Biotin/lipoate A/B protein ligase family [more]
AT1G04640.14.8e-3035.71lipoyltransferase 2 [more]
AT1G04640.24.8e-3035.71lipoyltransferase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004143Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domainPFAMPF03099BPL_LplA_LipBcoord: 109..217
e-value: 7.0E-8
score: 32.4
IPR004143Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domainPROSITEPS51733BPL_LPL_CATALYTICcoord: 86..282
score: 57.110569
IPR000544OctanoyltransferaseTIGRFAMTIGR00214TIGR00214coord: 83..243
e-value: 9.4E-56
score: 186.3
IPR000544OctanoyltransferaseHAMAPMF_00013LipBcoord: 51..281
score: 34.390823
IPR000544OctanoyltransferaseCDDcd16444LipBcoord: 61..278
e-value: 8.81665E-94
score: 273.983
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 49..256
e-value: 3.3E-68
score: 231.0
NoneNo IPR availablePIRSRPIRSR016262-1PIRSR016262-1coord: 58..247
e-value: 8.9E-63
score: 209.2
NoneNo IPR availablePIRSRPIRSR016262-2PIRSR016262-2coord: 58..247
e-value: 8.9E-63
score: 209.2
NoneNo IPR availablePIRSRPIRSR016262-3PIRSR016262-3coord: 58..247
e-value: 8.9E-63
score: 209.2
NoneNo IPR availablePANTHERPTHR10993OCTANOYLTRANSFERASEcoord: 46..291
NoneNo IPR availablePANTHERPTHR10993:SF14BNAC03G77390D PROTEINcoord: 46..291
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 51..245
IPR020605Octanoyltransferase, conserved sitePROSITEPS01313LIPBcoord: 128..143

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC09g1452.1MC09g1452.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009249 protein lipoylation
biological_process GO:0006464 cellular protein modification process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0033819 lipoyl(octanoyl) transferase activity
molecular_function GO:0102555 octanoyl transferase activity (acting on glycine-cleavage complex H protein)