MC09g1396 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g1396
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	4-coumarate--CoA ligase
Location	MC09: 20021828 .. 20025303 (+)
RNA-Seq Expression	MC09g1396
Synteny	MC09g1396

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTATATATTATCACGTAAATTGAATTGTTTTAGAATCACGATCGCCCGCCCAAATCTAACATGTCCGGCGAAGAATCGTACACTGCTCCGGCCGCCGGGGAATATCCACGGCAGAATGCTCCTCCGCCGCCGCTTTCAAAGGGCGGTTACGATGTGAATACTGCGGTTTATCATTCACTTGTCAAGTTCGATGAAGCTCGTAAGATCTCCAGCAGGCCAGATCTCGACACGGCCACTTTCGTCTTATCGCAGTTTCCGAAGGCCGAATCGCGAGTTGCTCTCATTGACTCGGTCACGAGCTCGCGAGTTACTTATGGACAACTCAGTGCCTCGATTCGCTCCCTCGCTTGCGGACTTCACCACGCGCTCGGAGTCAGAAAAGGCGATGTCGTGTTCGTTTTATCGGCGAATTCGATTCTGTATCCAGTTATCTGTTTGGCTGTGTTGTCGGTTGGTGCAGTGATAACAACGGCCAATCCAGTGAATACTGCGTCGGAAATCGCGAAGCAAGTGCGTGATTCAGGAGCGAAACTCGCCGTATCGGCGCCGGAGGAGATTCACAAACTTAATCCGACTGGAATTCCGACGATTCTGACTACTCGGCCTTCCTCTGGCGATGATGTTTTATCGGTGGAAGAGTTGATCGAATCCTGCGCCGACTCGTCTGAGTCATTGCCGAACACGGAAGTGACTCAGTCCGATACGGCGGCGATCTTGTACTCGTCGGGAACGACCGGGACGAGTAAAGGAGTAGTTCTAACGCATTCAAATCTCATCTCCGTTATCGAACTTCTAACCTTGTATGTGGACGCGAGTTCCTCTGAAAACGACGTGTTTTTGTGCTTCATTCCGATGTTTCACATCTACGGCCTCGTCTTCTTCGGCCTCGGACTCTTCTGCAGAGGAATCACCATCATTTTGATGCAGAGATTCAATTTCCAAGCCATGATCGACGCAATTGAAAAATACAAAGTTAACAACATACCAGCGGTACCTCCGGTGATTCTAGGGCTAGTGAAATCGGGCGGTGGTTCGGACTTATCATCGCTGAGACGCATCGGATCCGGCGCTGCACCGCTGGGAAAAGACATAGAAGACGCATTCCGAGAGAAATTTCCATGGGTGGAGCTTCGGCCTGGATATGGCTTAACAGAGAGTACTGGAGCAGCGACTTGCATTATTACAGACACAGACGCCAAAACTCATCCAGGTATCCAGAAATTCCTTCATCTGCGGGGGTAGAAATCAGTACAATAGACTTAGATTTTAATCAATTGTTCATATTCTTTCAATAAGGTTCGTGCGGAATGTTGATGCCGAGATTTTCTGCGAAGATCGTAGACATTGAAACAGGGGAGGGACTGCCGCCGATGAAGGAAGGCGAATTGTGGCTGAAGAGTCCAACAATAATGAAAGGATATCTCGGAAATCAGGAAGCAACAGAAGCCACCATGGATGAAGAAGGATGGTTGAAAACAGGGGATCTCGGGTACATAGACGAAGATGGCTTCCTTTACATCGTTGATCGAATCAAGGAGCTGATCAAGCACAATGGATACCAGGTACAAACAAAACACCTACCTGGTCCTTAAAATTATCATATAGCAATCTGATTAATGCCAAGAACTCTGCATCAATATTCATGGTTTGATTCTTATGGTTCTTCTGCTCTCACTAAATTTAGCTGCATTTCATTTTTGAACTGAATCATGGTAGTCCATATATCTTATAAATCTTGTTAGAAACTTGATCTTTTGGATGTTTAATACTCTGAAATCTGGATGCAGGTTGCTCCAGCAGAACTGGAAACGATTCTTGTGTGCCATACAGAAATTCTGGATGCTGCCGTTATACCGTGGGTTTAATTTAATCTCTTAAATCTCTCTCTAAATCTATAGAACAGTGAAGAGTGAGCACTGAGATTGTGTTGTATTGTATTGTGTTGCAGAATGGAGGATGAAGCCGCCGGGCAGATTCCGGTGGCTTGTGTGGTTAAAGCATCCACTAGTCAGCTTACAGAAGACCAAGTCATCCAATTTGTCGCTTCCCAGGTATTTTCTCTTTGCACCCACCTCTTAATTTTTCATTTAATTTTATTTATGGTTTTAATTAAATAGGCTTAACCTGCATTTTAGTGGGCCTGCCTTTAGCCCACGCAACCTTCCAAATATGTCTGGGCATCTTCTAATGCTTTAATTAGGGACTGTGAATATAGCATAATATGAATTAAATATGGTAGGAATAATAGTTTCAAAAGTAATTATAATTATGATAGAAAGGAAGGCTAATCAATTGAAAATTATACTTCAACCTGAGACATATATTTTTTATCAAAAAATAGGAGGTTTTAATTTTTGCCTACGTGTTGAACTTAAAAAAAAAAGAAAATTAAAAAATTATCAAATGTATCGTACTTTTGAAAGTGCATATATCTCCAATTTTTATATGAGGGAATTGAAAGCTAGAGTTTAATTATCTCATGAACTATAAAAACTACATAAACTTGGGCTTCTAAAACCGCAACTCAAGCTACATGCTTTTACAAATCACAAATCTACCCATCAAATACGGAATTTTATAACTTAATTCTATGTAAAATCAAATCATAAACTAAACACAAGGTCAAATTTTATAGTTCTAGATTACAGAAATCGAAGCCTATATCCATGAAGAAATTTGACATTTCTATACTTTCTATCAAACTTTTCAATAAATATAGGTCTTGCTAAGATATAGAGTATTAGTTTTAATTTTAATGATTTTTCAAAACAGACCAAATAATTTAAGGGCTAATTAAGTTTATATATATACACACACACAAATAGTCCTTAAAAGTTCTTCTAATTAGATAATGAACACTTTTTTTTTTATATTGTGATATTTGGTAACCCACCAAAAATTACATAAATATATATGTGCGCACGTGTTTAAAATTTTCTATCCAAAATTACGACAGTGGTAGCGAGAATGAGCTATATAGGCTTCAAGACTTGATCTCGATTTTACTCAACTAGGCCAATAAATGAGGGACTTTGTCAGATAATTTACATTCTTACCCTTACTATTAAACTCTAATTTTATTTTTTTTTTTGAAACTATTAAACTCTAAATTATTATTCATTATTCTGGATTTAATTTCAAATTTAAAATATTGCTTGAAATTATGAGGATAGTACTACTCGTAAAAATAAGTCTATCAATTTATAAGATACAACGAATACATATTCATATATATAAATATAATATAAAAAATGGCGAATAATACAATAATTGCTTTTTATTATTTATTATTTTTTTAATTTTTTTTATGTATGTGTGAGAAAATAAATCAAGTATAAAAAATTTTCAGGTGGCACCTTACAAGAAGGTAAGAGGAGTGAAATTTATCAATGCCATTCCAAGGTCCCTTGCAGGCAAAATTTTGAGGAAGGATCTTGTCTCACAATTCAAACAGCAACAGCTTTTCTCAAAACTC

mRNA sequence

CCTATATATTATCACGTAAATTGAATTGTTTTAGAATCACGATCGCCCGCCCAAATCTAACATGTCCGGCGAAGAATCGTACACTGCTCCGGCCGCCGGGGAATATCCACGGCAGAATGCTCCTCCGCCGCCGCTTTCAAAGGGCGGTTACGATGTGAATACTGCGGTTTATCATTCACTTGTCAAGTTCGATGAAGCTCGTAAGATCTCCAGCAGGCCAGATCTCGACACGGCCACTTTCGTCTTATCGCAGTTTCCGAAGGCCGAATCGCGAGTTGCTCTCATTGACTCGGTCACGAGCTCGCGAGTTACTTATGGACAACTCAGTGCCTCGATTCGCTCCCTCGCTTGCGGACTTCACCACGCGCTCGGAGTCAGAAAAGGCGATGTCGTGTTCGTTTTATCGGCGAATTCGATTCTGTATCCAGTTATCTGTTTGGCTGTGTTGTCGGTTGGTGCAGTGATAACAACGGCCAATCCAGTGAATACTGCGTCGGAAATCGCGAAGCAAGTGCGTGATTCAGGAGCGAAACTCGCCGTATCGGCGCCGGAGGAGATTCACAAACTTAATCCGACTGGAATTCCGACGATTCTGACTACTCGGCCTTCCTCTGGCGATGATGTTTTATCGGTGGAAGAGTTGATCGAATCCTGCGCCGACTCGTCTGAGTCATTGCCGAACACGGAAGTGACTCAGTCCGATACGGCGGCGATCTTGTACTCGTCGGGAACGACCGGGACGAGTAAAGGAGTAGTTCTAACGCATTCAAATCTCATCTCCGTTATCGAACTTCTAACCTTGTATGTGGACGCGAGTTCCTCTGAAAACGACGTGTTTTTGTGCTTCATTCCGATGTTTCACATCTACGGCCTCGTCTTCTTCGGCCTCGGACTCTTCTGCAGAGGAATCACCATCATTTTGATGCAGAGATTCAATTTCCAAGCCATGATCGACGCAATTGAAAAATACAAAGTTAACAACATACCAGCGGTACCTCCGGTGATTCTAGGGCTAGTGAAATCGGGCGGTGGTTCGGACTTATCATCGCTGAGACGCATCGGATCCGGCGCTGCACCGCTGGGAAAAGACATAGAAGACGCATTCCGAGAGAAATTTCCATGGGTGGAGCTTCGGCCTGGATATGGCTTAACAGAGAGTACTGGAGCAGCGACTTGCATTATTACAGACACAGACGCCAAAACTCATCCAGGTTCGTGCGGAATGTTGATGCCGAGATTTTCTGCGAAGATCGTAGACATTGAAACAGGGGAGGGACTGCCGCCGATGAAGGAAGGCGAATTGTGGCTGAAGAGTCCAACAATAATGAAAGGATATCTCGGAAATCAGGAAGCAACAGAAGCCACCATGGATGAAGAAGGATGGTTGAAAACAGGGGATCTCGGGTACATAGACGAAGATGGCTTCCTTTACATCGTTGATCGAATCAAGGAGCTGATCAAGCACAATGGATACCAGGTTGCTCCAGCAGAACTGGAAACGATTCTTGTGTGCCATACAGAAATTCTGGATGCTGCCGTTATACCAATGGAGGATGAAGCCGCCGGGCAGATTCCGGTGGCTTGTGTGGTTAAAGCATCCACTAGTCAGCTTACAGAAGACCAAGTCATCCAATTTGTCGCTTCCCAGGTGGCACCTTACAAGAAGGTAAGAGGAGTGAAATTTATCAATGCCATTCCAAGGTCCCTTGCAGGCAAAATTTTGAGGAAGGATCTTGTCTCACAATTCAAACAGCAACAGCTTTTCTCAAAACTC

Coding sequence (CDS)

ATGTCCGGCGAAGAATCGTACACTGCTCCGGCCGCCGGGGAATATCCACGGCAGAATGCTCCTCCGCCGCCGCTTTCAAAGGGCGGTTACGATGTGAATACTGCGGTTTATCATTCACTTGTCAAGTTCGATGAAGCTCGTAAGATCTCCAGCAGGCCAGATCTCGACACGGCCACTTTCGTCTTATCGCAGTTTCCGAAGGCCGAATCGCGAGTTGCTCTCATTGACTCGGTCACGAGCTCGCGAGTTACTTATGGACAACTCAGTGCCTCGATTCGCTCCCTCGCTTGCGGACTTCACCACGCGCTCGGAGTCAGAAAAGGCGATGTCGTGTTCGTTTTATCGGCGAATTCGATTCTGTATCCAGTTATCTGTTTGGCTGTGTTGTCGGTTGGTGCAGTGATAACAACGGCCAATCCAGTGAATACTGCGTCGGAAATCGCGAAGCAAGTGCGTGATTCAGGAGCGAAACTCGCCGTATCGGCGCCGGAGGAGATTCACAAACTTAATCCGACTGGAATTCCGACGATTCTGACTACTCGGCCTTCCTCTGGCGATGATGTTTTATCGGTGGAAGAGTTGATCGAATCCTGCGCCGACTCGTCTGAGTCATTGCCGAACACGGAAGTGACTCAGTCCGATACGGCGGCGATCTTGTACTCGTCGGGAACGACCGGGACGAGTAAAGGAGTAGTTCTAACGCATTCAAATCTCATCTCCGTTATCGAACTTCTAACCTTGTATGTGGACGCGAGTTCCTCTGAAAACGACGTGTTTTTGTGCTTCATTCCGATGTTTCACATCTACGGCCTCGTCTTCTTCGGCCTCGGACTCTTCTGCAGAGGAATCACCATCATTTTGATGCAGAGATTCAATTTCCAAGCCATGATCGACGCAATTGAAAAATACAAAGTTAACAACATACCAGCGGTACCTCCGGTGATTCTAGGGCTAGTGAAATCGGGCGGTGGTTCGGACTTATCATCGCTGAGACGCATCGGATCCGGCGCTGCACCGCTGGGAAAAGACATAGAAGACGCATTCCGAGAGAAATTTCCATGGGTGGAGCTTCGGCCTGGATATGGCTTAACAGAGAGTACTGGAGCAGCGACTTGCATTATTACAGACACAGACGCCAAAACTCATCCAGGTTCGTGCGGAATGTTGATGCCGAGATTTTCTGCGAAGATCGTAGACATTGAAACAGGGGAGGGACTGCCGCCGATGAAGGAAGGCGAATTGTGGCTGAAGAGTCCAACAATAATGAAAGGATATCTCGGAAATCAGGAAGCAACAGAAGCCACCATGGATGAAGAAGGATGGTTGAAAACAGGGGATCTCGGGTACATAGACGAAGATGGCTTCCTTTACATCGTTGATCGAATCAAGGAGCTGATCAAGCACAATGGATACCAGGTTGCTCCAGCAGAACTGGAAACGATTCTTGTGTGCCATACAGAAATTCTGGATGCTGCCGTTATACCAATGGAGGATGAAGCCGCCGGGCAGATTCCGGTGGCTTGTGTGGTTAAAGCATCCACTAGTCAGCTTACAGAAGACCAAGTCATCCAATTTGTCGCTTCCCAGGTGGCACCTTACAAGAAGGTAAGAGGAGTGAAATTTATCAATGCCATTCCAAGGTCCCTTGCAGGCAAAATTTTGAGGAAGGATCTTGTCTCACAATTCAAACAGCAACAGCTTTTCTCAAAACTC

Protein sequence

MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVNTAVYHSLVKFDEARKISSRPDLDTATFVLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSILYPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTTRPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAIEKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPGYGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVKFINAIPRSLAGKILRKDLVSQFKQQQLFSKL

Homology

[Close]

BLAST of MC09g1396 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 3.3e-125
Identity = 249/546 (45.60%), Postives = 354/546 (64.84%), Query Frame = 0


Query: 27  KGGYDVNTAVYHSLVKFDEARKISSRPDLDTATFVLSQFPKAESRVALIDSVTSSRVTYG 86
           + GY  + ++++S     E  ++     +D  TF+ S+      ++A ID+ T   +T+ 
Sbjct: 12  RSGYCKSNSIFYSK---REPVQLPQNHSVDVTTFISSR--AHHGKIAFIDAATGRHLTFP 71

Query: 87  QLSASIRSLACGLHHALGVRKGDVVFVLSANSILYPVICLAVLSVGAVITTANPVNTASE 146
           QL  ++ S+A  L  A+G+RKGDV+ +LS NSI +PV+CLAV+S+GA+ITT NP+NT  E
Sbjct: 72  QLWRAVDSVATCL-SAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPRE 131

Query: 147 IAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTTRPSSGDDVLSVEE----LIESCAD-- 206
           IAKQ+ DS   LA + P+ + K+  + +P ++       D+V S  E    ++ S  +  
Sbjct: 132 IAKQITDSKPVLAFTIPQLVSKIAGSNLPIVII-----DDEVKSSLEKTLNIVSSLGEMM 191

Query: 207 SSESLPNT---EVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTLYVDASSSEND 266
             E  PN     V Q DTA +LYSSGTTG SKGVV +H NLI++++ +         E+ 
Sbjct: 192 RKEPSPNRIGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIAMVQTILSRFGTEDGEH- 251

Query: 267 VFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAIEKYKVNNIPAVPPVILG 326
            F+C +PMFHIYGL  F +GL   G TI+++ +F    M+ AIEKY+   +P VPP+++ 
Sbjct: 252 TFICTVPMFHIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMA 311

Query: 327 LVKSG----GGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPGYGLTESTGAATCI 386
           L+K+        DLSSL+ + SG APL K++ + F E +P V +  GYGLTESTG     
Sbjct: 312 LLKNANHIRAKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGAST 371

Query: 387 ITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNQEATE 446
               +++ + G+ GML P   AKIV+ ETGE L   + GELWL+ PTIMKGY  N+EAT 
Sbjct: 372 DCLQESRRY-GTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEATS 431

Query: 447 ATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILVCHTEILDAAV 506
           +T+D EGWL+TGDL YIDEDGF+++VDR+KELIK+ GYQVAPAELE +L+ H EI DAAV
Sbjct: 432 STIDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDAAV 491

Query: 507 IPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVKFINAIPRSLAGKI 560
           IP  D+ AGQ P+A VV+   S L+E  V+ F+A  VAPYK++R V F+ +IP++ +GKI
Sbjct: 492 IPYPDKEAGQFPMAYVVRKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSGKI 544

[Close]

BLAST of MC09g1396 vs. ExPASy Swiss-Prot
Match: Q84P21 (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 441.8 bits (1135), Expect = 1.2e-122
Identity = 239/515 (46.41%), Postives = 343/515 (66.60%), Query Frame = 0


Query: 53  PDLDTATFVLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVF 112
           P LD  TF+ SQ  +   R+A ID+ T   +T+ +L  ++ S+A  L   +G+RKG VV 
Sbjct: 30  PSLDVTTFISSQAHR--GRIAFIDASTGQNLTFTELWRAVESVADCLSE-IGIRKGHVVL 89

Query: 113 VLSANSILYPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPT 172
           +LS NSIL+PV+CL+V+S+GA+ITT NP+NT++EIAKQ++DS   LA +  + + K++  
Sbjct: 90  LLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAA 149

Query: 173 G--IPTIL--TTRPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTS 232
              +P +L    R  S  DV  + E+++   + S +     V Q DTA +LYSSGTTG S
Sbjct: 150 AKKLPIVLMDEERVDSVGDVRRLVEMMKK--EPSGNRVKERVDQDDTATLLYSSGTTGMS 209

Query: 233 KGVVLTHSNLISVIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILM 292
           KGV+ +H NLI++++ +     +   E   F+C +PMFHIYGL  F  GL   G TII++
Sbjct: 210 KGVISSHRNLIAMVQTIVNRFGSDDGEQR-FICTVPMFHIYGLAAFATGLLAYGSTIIVL 269

Query: 293 QRFNFQAMIDAIEKYKVNNIPAVPPVILGLVKSG----GGSDLSSLRRIGSGAAPLGKDI 352
            +F    M+ AI KY+  ++P VPP+++ +V          DLSS+  +  G APL K++
Sbjct: 270 SKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEV 329

Query: 353 EDAFREKFPWVELRPGYGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGE 412
            + F EK+P V++  GYGLTESTG      T  +++ + G+ G L      +IVD  TG+
Sbjct: 330 TEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRY-GTAGKLSASMEGRIVDPVTGQ 389

Query: 413 GLPPMKEGELWLKSPTIMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKE 472
            L P + GELWLK P+IMKGY  N+EAT +T+D EGWL+TGDL YIDEDGF+++VDR+KE
Sbjct: 390 ILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKE 449

Query: 473 LIKHNGYQVAPAELETILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQ 532
           LIK+ GYQVAPAELE +L+ H EI DAAVIP  D+  GQ P+A VV+ + S L+E  +++
Sbjct: 450 LIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIME 509

Query: 533 FVASQVAPYKKVRGVKFINAIPRSLAGKILRKDLV 560
           FVA QVAPYK++R V F+++IP++ +GKILRKDL+
Sbjct: 510 FVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLI 537

[Close]

BLAST of MC09g1396 vs. ExPASy Swiss-Prot
Match: Q9M0X9 (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 5.8e-122
Identity = 241/550 (43.82%), Postives = 344/550 (62.55%), Query Frame = 0


Query: 25  LSKGGYDVNTAVYHSLVKFDEARKISSRPDLDTATFVLSQFPKAESRVALIDSVTSSRVT 84
           + K GY     +Y SL        +   P+    +F+        S++A+ DS T   +T
Sbjct: 1   MEKSGYG-RDGIYRSL---RPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLT 60

Query: 85  YGQLSASIRSLACGLHHALGVRKGDVVFVLSANSILYPVICLAVLSVGAVITTANPVNTA 144
           + QL +++  LA G H  LG+RK DVV + + NS  +P+  LAV ++G V TTANP+ T 
Sbjct: 61  FSQLKSAVARLAHGFHR-LGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTV 120

Query: 145 SEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTIL-------TTRPSSGDDVLSVEELIES 204
           +E++KQ++DS  K+ +S  +   K+    +P +L          P S   +LS + ++E 
Sbjct: 121 NEVSKQIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMEL 180

Query: 205 CADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTLYVDASSSEND 264
               SE  P  E+ QSDTAA+LYSSGTTGTSKGV LTH N I+   ++T+  D     + 
Sbjct: 181 SEPVSE-YPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHG 240

Query: 265 VFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAIEKYKVNNIPAVPPVILG 324
           VFLCF+PMFH++GL         RG  ++ M RF  + ++  IEK++V ++  VPPV L 
Sbjct: 241 VFLCFLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLA 300

Query: 325 LVKSG--GGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPGYGLTESTGAATCIIT 384
           L K       DLSSL+ IGSGAAPLGKD+ +      P V L  GYG+TE+ G  + +  
Sbjct: 301 LSKQSIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVS-VED 360

Query: 385 DTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNQEATEAT 444
               K + GS GML P   A+IV +ETG+  PP ++GE+W++ P +MKGYL N +AT+ T
Sbjct: 361 PRLGKRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKET 420

Query: 445 MDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILVCHTEILDAAVIP 504
           +D++ W+ TGDLGY +EDG LY+VDRIKELIK+ G+QVAPAELE +LV H +ILDA VIP
Sbjct: 421 IDKKSWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIP 480

Query: 505 MEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVKFINAIPRSLAGKILR 564
             DE AG++P+A VV++  S +TE  + +F+A QVAPYK++R V FI+ +P+S AGKILR
Sbjct: 481 FPDEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILR 540

Query: 565 KDLVSQFKQQ 566
           ++LV Q + +
Sbjct: 541 RELVQQVRSK 543

[Close]

BLAST of MC09g1396 vs. ExPASy Swiss-Prot
Match: Q10S72 (4-coumarate--CoA ligase-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL4 PE=2 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 5.1e-118
Identity = 232/516 (44.96%), Postives = 337/516 (65.31%), Query Frame = 0


Query: 53  PDLDTATFVLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGL-HHALGVRKGDVV 112
           P++D  +F+ S+  +    VAL+D+ T  R+T+ +L  ++   A  L  H + +RKG V 
Sbjct: 35  PEVDVVSFLASR--RHSGVVALVDAATGRRITFTELWRAVAGAASALAAHPVSLRKGHVA 94

Query: 113 FVLSANSILYPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKL-- 172
            +LS NS+ +PV  LA +S+GAV+TTANP+NT +EIAKQV D+   LA +  E + KL  
Sbjct: 95  LILSPNSVHFPVAALAAMSLGAVLTTANPLNTPAEIAKQVADARPVLAFTTRELLPKLPR 154

Query: 173 -NPTGIPTILTTR-PSSGDD---VLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGT 232
            +   +  + + R P    D   V ++EE+  +  D +       VTQ D A +LYSSGT
Sbjct: 155 AHDLRVVLLESARLPGDSSDPRIVATIEEISATTPDPARR--KDRVTQDDPATLLYSSGT 214

Query: 233 TGTSKGVVLTHSNLISVIE-LLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGI 292
           TG SKGVV TH +LIS+++ ++T +    S + + FLC +PMFH+YGLV F  GL   G 
Sbjct: 215 TGPSKGVVATHRSLISMVQIIMTRFRLEGSDKTETFLCTVPMFHVYGLVAFATGLLGCGA 274

Query: 293 TIILMQRFNFQAMIDAIEKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKD 352
           T++++ ++    M+ +I  Y V  +P VPP+++ +V       L  +R++ SG APLGK+
Sbjct: 275 TVVVLSKYELPEMLRSINAYGVTYLPLVPPILVAMVAHPKPLPLGQMRKVLSGGAPLGKE 334

Query: 353 IEDAFREKFPWVELRPGYGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETG 412
           + + FREK+P VE+  GYGLTEST       +  +++ + G+ G+L P   AKIVD ++G
Sbjct: 335 LIEGFREKYPQVEILQGYGLTESTAIGASTDSAEESRRY-GTAGLLSPNTEAKIVDPDSG 394

Query: 413 EGLPPMKEGELWLKSPTIMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIK 472
           E LP  + GELW++ P +MKGY  N EAT++T+  +GWLKTGDL YIDEDG+L++VDR+K
Sbjct: 395 EALPVNRTGELWIRGPYVMKGYFKNAEATQSTLTPDGWLKTGDLCYIDEDGYLFVVDRLK 454

Query: 473 ELIKHNGYQVAPAELETILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVI 532
           ELIK+ GYQV PAELE +L+ H E+ D AVIP  D   GQ P+A +V+   S L+E +V+
Sbjct: 455 ELIKYKGYQVPPAELEALLLTHPEVTDVAVIPFPDREVGQFPMAYIVRKKGSNLSEREVM 514

Query: 533 QFVASQVAPYKKVRGVKFINAIPRSLAGKILRKDLV 560
           +FVA QVAPYKKVR V F+  IP++ +GKILRKDL+
Sbjct: 515 EFVAKQVAPYKKVRKVAFVTDIPKNASGKILRKDLI 545

[Close]

BLAST of MC09g1396 vs. ExPASy Swiss-Prot
Match: M4IRL6 (Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 5.1e-118
Identity = 239/521 (45.87%), Postives = 339/521 (65.07%), Query Frame = 0


Query: 54  DLDTATFVLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFV 113
           +L   +FV         + ALIDS T+  +++ Q  + +  ++ G  + LGV+K DVV +
Sbjct: 26  NLSMVSFVFRNSSSYPQKPALIDSDTNETLSFSQFKSMVIKVSHGFLN-LGVQKNDVVLI 85

Query: 114 LSANSILYPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTG 173
            + NSI  PV  L +++ GA+ TT+NP+ T SE++KQV+DS  KL V+ PE   K+    
Sbjct: 86  FAPNSIHVPVCFLGIVASGAIATTSNPLYTVSELSKQVKDSNPKLIVTVPELFEKVKGFN 145

Query: 174 IPTILTTRPSSGDD--------VLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTT 233
           +PTIL   P+S D         VL+  +L+      S+  P  +  QSDTAA+LYSSGTT
Sbjct: 146 LPTIL-IGPNSEDSSPLKSRAKVLTFHDLVTLSGPVSD-FPMVDFKQSDTAALLYSSGTT 205

Query: 234 GTSKGVVLTHSNLISVIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITI 293
           G SKGVVL+H N I+   ++T+  D +   ++VFLCF+PMFH++GL         RG T+
Sbjct: 206 GMSKGVVLSHKNFIASSLMVTMEQDQAGEMHNVFLCFLPMFHVFGLAIITYAQLQRGNTV 265

Query: 294 ILMQRFNFQAMIDAIEKYKVNNIPAVPPVILGLVKSG--GGSDLSSLRRIGSGAAPLGKD 353
           I M RF+ + ++  +EKYKV ++  VPPVIL L K+      DLSSL+ IGSGAAPLGKD
Sbjct: 266 ISMARFDLEKILKDVEKYKVTHLWVVPPVILALTKNSIVKKYDLSSLKHIGSGAAPLGKD 325

Query: 354 IEDAFREKFPWVELRPGYGLTESTGAATCIITDT-DAKTHPGSCGMLMPRFSAKIVDIET 413
           + +   +  P   +  GYG+TE+ G  +  + DT   K H GS GML     A+IV ++T
Sbjct: 326 LMEECAKIVPHGIVAQGYGMTETCGIVS--VEDTRGGKRHTGSAGMLSSGVEAQIVSVDT 385

Query: 414 GEGLPPMKEGELWLKSPTIMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRI 473
            + LPP + GE+W++ P +M+GY  N  AT+ T+D++GW+ TGDLGY DEDG LY+VDRI
Sbjct: 386 LKPLPPNQLGEIWVRGPNMMQGYFNNPRATKLTIDKKGWVHTGDLGYFDEDGHLYVVDRI 445

Query: 474 KELIKHNGYQVAPAELETILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQV 533
           KELIK+ G+QVAPAELE +LV H EILDA VIP  D  AG++PVA VV++  S LTED V
Sbjct: 446 KELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDADAGEVPVAYVVRSPNSSLTEDDV 505

Query: 534 IQFVASQVAPYKKVRGVKFINAIPRSLAGKILRKDLVSQFK 564
            +F+A QVA +K++R V FIN++P+S +GKILR++L+ + +
Sbjct: 506 KKFIAGQVASFKRLRKVTFINSVPKSASGKILRRELIQKVR 541

[Close]

BLAST of MC09g1396 vs. NCBI nr
Match: XP_022138968.1 (4-coumarate--CoA ligase-like 5 [Momordica charantia])

HSP 1 Score: 1103 bits (2853), Expect = 0.0
Identity = 570/571 (99.82%), Postives = 570/571 (99.82%), Query Frame = 0


Query: 1   MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVNTAVYHSLVKFDEARKISSRPDLDTATF 60
           MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVN AVYHSLVKFDEARKISSRPDLDTATF
Sbjct: 1   MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVNIAVYHSLVKFDEARKISSRPDLDTATF 60

Query: 61  VLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSIL 120
           VLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSIL
Sbjct: 61  VLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSIL 120

Query: 121 YPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTT 180
           YPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTT
Sbjct: 121 YPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTT 180

Query: 181 RPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240
           RPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS
Sbjct: 181 RPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240

Query: 241 VIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAI 300
           VIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAI
Sbjct: 241 VIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAI 300

Query: 301 EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPG 360
           EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPG
Sbjct: 301 EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPG 360

Query: 361 YGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPT 420
           YGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPT
Sbjct: 361 YGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPT 420

Query: 421 IMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480
           IMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET
Sbjct: 421 IMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480

Query: 481 ILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVK 540
           ILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVK
Sbjct: 481 ILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVK 540

Query: 541 FINAIPRSLAGKILRKDLVSQFKQQQLFSKL 571
           FINAIPRSLAGKILRKDLVSQFKQQQLFSKL
Sbjct: 541 FINAIPRSLAGKILRKDLVSQFKQQQLFSKL 571

[Close]

BLAST of MC09g1396 vs. NCBI nr
Match: XP_038891768.1 (4-coumarate--CoA ligase-like 5 [Benincasa hispida])

HSP 1 Score: 942 bits (2434), Expect = 0.0
Identity = 489/571 (85.64%), Postives = 528/571 (92.47%), Query Frame = 0


Query: 1   MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVNTAVYHSLVKFDEARKISSRPDLDTATF 60
           MSGEES TAPAA E+  QN+P      GGY+V TAVYHSL++ DEA  IS+R DL+TATF
Sbjct: 1   MSGEESCTAPAAVEFQPQNSP------GGYNVKTAVYHSLIQLDEAPIISTRQDLNTATF 60

Query: 61  VLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSIL 120
           VLSQFP AESRVALIDSVTSSRVTYGQLS SIRSLACGL+HALGVRKGDVVFVLSANS+L
Sbjct: 61  VLSQFPTAESRVALIDSVTSSRVTYGQLSVSIRSLACGLYHALGVRKGDVVFVLSANSVL 120

Query: 121 YPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTT 180
           YPVICLAV+S+GAVITTANP+NTASEI KQVRDSGAKLAVSAPEE+HKL+PTGIPTILTT
Sbjct: 121 YPVICLAVMSIGAVITTANPINTASEIGKQVRDSGAKLAVSAPEELHKLSPTGIPTILTT 180

Query: 181 RPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240
           R S GD  LSVEELIESC++S E LP  EVTQSD AAILYSSGTTGTSKGVVLTHSN+IS
Sbjct: 181 RSSYGD-TLSVEELIESCSESFEPLPKIEVTQSDPAAILYSSGTTGTSKGVVLTHSNIIS 240

Query: 241 VIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAI 300
           VIELLT  VD++SS++DVFLCFIPMFHIYGLVFFGLGLFCRGIT +LMQRFNFQ+MI AI
Sbjct: 241 VIELLTWCVDSTSSQHDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIAAI 300

Query: 301 EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPG 360
           EK+K+NNIPAVPPVILGLVKSG GSD SSLRR+GSGAAPLGKD+E+AFREKFPWVELRPG
Sbjct: 301 EKHKINNIPAVPPVILGLVKSGDGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPG 360

Query: 361 YGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPT 420
           YGLTESTGAAT +ITD DAK HPGSCGMLMP F AKIVD+ETGEG+ PMKEGELWLKSPT
Sbjct: 361 YGLTESTGAATWMITDKDAKAHPGSCGMLMPSFFAKIVDVETGEGVAPMKEGELWLKSPT 420

Query: 421 IMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480
           IMKGYLGN+EATEAT+DEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET
Sbjct: 421 IMKGYLGNKEATEATIDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480

Query: 481 ILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVK 540
           IL+ HTEILDAAVIPMEDEAAGQIPVACVVKA TS+LTE+QVIQFVASQVA YKKVRGV+
Sbjct: 481 ILLSHTEILDAAVIPMEDEAAGQIPVACVVKAPTSKLTEEQVIQFVASQVAAYKKVRGVR 540

Query: 541 FINAIPRSLAGKILRKDLVSQFKQQQLFSKL 571
           FI++IPRSLAGKILRKDLVSQFKQQQL SKL
Sbjct: 541 FISSIPRSLAGKILRKDLVSQFKQQQLLSKL 564

[Close]

BLAST of MC09g1396 vs. NCBI nr
Match: XP_022994494.1 (4-coumarate--CoA ligase-like 5 [Cucurbita maxima])

HSP 1 Score: 941 bits (2432), Expect = 0.0
Identity = 488/571 (85.46%), Postives = 526/571 (92.12%), Query Frame = 0


Query: 1   MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVNTAVYHSLVKFDEARKISSRPDLDTATF 60
           MS EES TAPAA E+   N+P      GGYDVNTAVYHS V+ DEA KIS+ PDLDTATF
Sbjct: 1   MSREESCTAPAAVEFRPMNSP------GGYDVNTAVYHSFVQLDEAPKISTSPDLDTATF 60

Query: 61  VLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSIL 120
           VLSQFP AESRVALIDSVTSSRVTYG+LSAS RSLA GL+HALGVRKGDVVFVLSANS+L
Sbjct: 61  VLSQFPTAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVL 120

Query: 121 YPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTT 180
           YPVICLAVLS+GAVITTANPVNTASEI KQV DS AKLA+SAPEE+HKL+PTGIPTILTT
Sbjct: 121 YPVICLAVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTT 180

Query: 181 RPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240
           +PS GD VLSVEELIESC +S E +P T++ QSD AAILYSSGTTGTSKGVVLTHSNLIS
Sbjct: 181 QPSYGD-VLSVEELIESCIESFEQMPETKIAQSDAAAILYSSGTTGTSKGVVLTHSNLIS 240

Query: 241 VIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAI 300
           VIELLT YVDASSS++DVFLCF+PMFHIYGLVFFGLGLFCRG TI+LMQRFNFQ+MIDAI
Sbjct: 241 VIELLTWYVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAI 300

Query: 301 EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPG 360
           E YKVNNIPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKD+E+AFREKFPWVELRPG
Sbjct: 301 ETYKVNNIPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPG 360

Query: 361 YGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPT 420
           YGLTESTGAATC+ITD DA+ HPGSCGMLMPRF AKIVDIETGEGLPPMKEGE+WLKSPT
Sbjct: 361 YGLTESTGAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPT 420

Query: 421 IMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480
           IMK YL N+EATEATMD+EGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET
Sbjct: 421 IMKEYLRNKEATEATMDKEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480

Query: 481 ILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVK 540
           IL+ HTEILDAAVIP EDEAAGQIPVACVVKA + +LTE+QVIQFVASQVA YKK+RGV+
Sbjct: 481 ILLSHTEILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVR 540

Query: 541 FINAIPRSLAGKILRKDLVSQFKQQQLFSKL 571
           F+++IPRSLAGKILRKDLVSQFKQQQ+ SKL
Sbjct: 541 FMSSIPRSLAGKILRKDLVSQFKQQQVLSKL 564

[Close]

BLAST of MC09g1396 vs. NCBI nr
Match: XP_022957594.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 937 bits (2423), Expect = 0.0
Identity = 486/567 (85.71%), Postives = 524/567 (92.42%), Query Frame = 0


Query: 1   MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVNTAVYHSLVKFDEARKISSRPDLDTATF 60
           MS EES TAPAA E+   N+P      GGYDVNTAVYHSLV+FDEA KIS+ PDLDTATF
Sbjct: 1   MSREESCTAPAAVEFRPINSP------GGYDVNTAVYHSLVQFDEAPKISTSPDLDTATF 60

Query: 61  VLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSIL 120
           VLSQFP AESRVALIDSVTSSRVTYG+LSAS RSLA GL+HALGVRKGDVVFVLSANS+L
Sbjct: 61  VLSQFPTAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVL 120

Query: 121 YPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTT 180
           YPVICLAVLS+GAVITTANPVNTASEI KQV DS AKLA+SAPEE+HKL+PTG+PTILTT
Sbjct: 121 YPVICLAVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGVPTILTT 180

Query: 181 RPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240
           RPS GD VLSVEELIESC +S E +P T++ QSDTAAILYSSGTTGTSKGVVLTHSNLIS
Sbjct: 181 RPSYGD-VLSVEELIESCIESFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240

Query: 241 VIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAI 300
           VIELLT YVDASSS++DVFLCF+PMFHIYGLVFFGLGLFCRG TI+LMQRFNFQ+MIDAI
Sbjct: 241 VIELLTWYVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAI 300

Query: 301 EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPG 360
           E YKVNNIPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKD+E+AFREKFPWVELRPG
Sbjct: 301 ETYKVNNIPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPG 360

Query: 361 YGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPT 420
           YGLTESTGAATC+ITD DA+ HPGSCGMLMPRF AKIVDIETGEGLPPMKEGE+WLKSPT
Sbjct: 361 YGLTESTGAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPT 420

Query: 421 IMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480
           IMK YL N+EATEATMD+EGW+KTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET
Sbjct: 421 IMKEYLRNKEATEATMDKEGWMKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480

Query: 481 ILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVK 540
           IL+ HTEILDAAVIP EDEAAGQIPVACVVKA + +LTE+QVIQFVASQVA YKK+RGV+
Sbjct: 481 ILLSHTEILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVR 540

Query: 541 FINAIPRSLAGKILRKDLVSQFKQQQL 567
           F+++IPRSLAGKILRKDLVSQ KQQ L
Sbjct: 541 FMSSIPRSLAGKILRKDLVSQLKQQVL 560

[Close]

BLAST of MC09g1396 vs. NCBI nr
Match: KAG7031989.1 (4-coumarate--CoA ligase-like 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 936 bits (2419), Expect = 0.0
Identity = 485/567 (85.54%), Postives = 523/567 (92.24%), Query Frame = 0


Query: 1   MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVNTAVYHSLVKFDEARKISSRPDLDTATF 60
           MS EES TAPAA E+   N+P      GGYDVNTAVYHSLV+ DEA KIS+ PDLDTATF
Sbjct: 1   MSREESCTAPAAVEFRPMNSP------GGYDVNTAVYHSLVQLDEASKISTSPDLDTATF 60

Query: 61  VLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSIL 120
           VLSQFP AESRVALIDSVTSSRVTYG+LSAS RSLA GL+HALGVRKGDVVFVLSANS+L
Sbjct: 61  VLSQFPTAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVL 120

Query: 121 YPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTT 180
           YPVICLAVLS+GAVITTANPVNTASEI KQV DS AKLA+SAPEE+HKL+PTGIPTILTT
Sbjct: 121 YPVICLAVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTT 180

Query: 181 RPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240
           RPS GD VLSVEELIESC ++ E +P T++ QSDTAAILYSSGTTGTSKGVVLTHSNLIS
Sbjct: 181 RPSYGD-VLSVEELIESCIETFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240

Query: 241 VIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAI 300
           VIELLT YVDASSS++DVFLCF+PMFHIYGLVFFGLGLFCRG TI+LMQRFNFQ+MIDAI
Sbjct: 241 VIELLTWYVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAI 300

Query: 301 EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPG 360
           E YKVNNIPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKD+E+AFREKFPWVELRPG
Sbjct: 301 ETYKVNNIPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPG 360

Query: 361 YGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPT 420
           YGLTESTGAATC+ITD DA+ HPGSCGMLMPRF AKIVDIETGEGLPPMKEGE+WLKSPT
Sbjct: 361 YGLTESTGAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPT 420

Query: 421 IMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480
           IMK YL N+EATEATMD+EGW+KTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET
Sbjct: 421 IMKEYLRNKEATEATMDKEGWMKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480

Query: 481 ILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVK 540
           IL+ HTEILDAAVIP EDEAAGQIPVACVVKA + +LTE+QVIQFVASQVA YKK+RGV+
Sbjct: 481 ILLSHTEILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVR 540

Query: 541 FINAIPRSLAGKILRKDLVSQFKQQQL 567
           F+++IPRSLAGKILRKDLVSQ KQQ L
Sbjct: 541 FMSSIPRSLAGKILRKDLVSQLKQQVL 560

[Close]

BLAST of MC09g1396 vs. ExPASy TrEMBL
Match: A0A6J1CEK1 (4-coumarate--CoA ligase-like 5 OS=Momordica charantia OX=3673 GN=LOC111010017 PE=4 SV=1)

HSP 1 Score: 1103 bits (2853), Expect = 0.0
Identity = 570/571 (99.82%), Postives = 570/571 (99.82%), Query Frame = 0


Query: 1   MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVNTAVYHSLVKFDEARKISSRPDLDTATF 60
           MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVN AVYHSLVKFDEARKISSRPDLDTATF
Sbjct: 1   MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVNIAVYHSLVKFDEARKISSRPDLDTATF 60

Query: 61  VLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSIL 120
           VLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSIL
Sbjct: 61  VLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSIL 120

Query: 121 YPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTT 180
           YPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTT
Sbjct: 121 YPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTT 180

Query: 181 RPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240
           RPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS
Sbjct: 181 RPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240

Query: 241 VIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAI 300
           VIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAI
Sbjct: 241 VIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAI 300

Query: 301 EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPG 360
           EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPG
Sbjct: 301 EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPG 360

Query: 361 YGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPT 420
           YGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPT
Sbjct: 361 YGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPT 420

Query: 421 IMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480
           IMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET
Sbjct: 421 IMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480

Query: 481 ILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVK 540
           ILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVK
Sbjct: 481 ILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVK 540

Query: 541 FINAIPRSLAGKILRKDLVSQFKQQQLFSKL 571
           FINAIPRSLAGKILRKDLVSQFKQQQLFSKL
Sbjct: 541 FINAIPRSLAGKILRKDLVSQFKQQQLFSKL 571

[Close]

BLAST of MC09g1396 vs. ExPASy TrEMBL
Match: A0A6J1K303 (4-coumarate--CoA ligase-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111490173 PE=4 SV=1)

HSP 1 Score: 941 bits (2432), Expect = 0.0
Identity = 488/571 (85.46%), Postives = 526/571 (92.12%), Query Frame = 0


Query: 1   MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVNTAVYHSLVKFDEARKISSRPDLDTATF 60
           MS EES TAPAA E+   N+P      GGYDVNTAVYHS V+ DEA KIS+ PDLDTATF
Sbjct: 1   MSREESCTAPAAVEFRPMNSP------GGYDVNTAVYHSFVQLDEAPKISTSPDLDTATF 60

Query: 61  VLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSIL 120
           VLSQFP AESRVALIDSVTSSRVTYG+LSAS RSLA GL+HALGVRKGDVVFVLSANS+L
Sbjct: 61  VLSQFPTAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVL 120

Query: 121 YPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTT 180
           YPVICLAVLS+GAVITTANPVNTASEI KQV DS AKLA+SAPEE+HKL+PTGIPTILTT
Sbjct: 121 YPVICLAVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGIPTILTT 180

Query: 181 RPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240
           +PS GD VLSVEELIESC +S E +P T++ QSD AAILYSSGTTGTSKGVVLTHSNLIS
Sbjct: 181 QPSYGD-VLSVEELIESCIESFEQMPETKIAQSDAAAILYSSGTTGTSKGVVLTHSNLIS 240

Query: 241 VIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAI 300
           VIELLT YVDASSS++DVFLCF+PMFHIYGLVFFGLGLFCRG TI+LMQRFNFQ+MIDAI
Sbjct: 241 VIELLTWYVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAI 300

Query: 301 EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPG 360
           E YKVNNIPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKD+E+AFREKFPWVELRPG
Sbjct: 301 ETYKVNNIPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPG 360

Query: 361 YGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPT 420
           YGLTESTGAATC+ITD DA+ HPGSCGMLMPRF AKIVDIETGEGLPPMKEGE+WLKSPT
Sbjct: 361 YGLTESTGAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPT 420

Query: 421 IMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480
           IMK YL N+EATEATMD+EGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET
Sbjct: 421 IMKEYLRNKEATEATMDKEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480

Query: 481 ILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVK 540
           IL+ HTEILDAAVIP EDEAAGQIPVACVVKA + +LTE+QVIQFVASQVA YKK+RGV+
Sbjct: 481 ILLSHTEILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVR 540

Query: 541 FINAIPRSLAGKILRKDLVSQFKQQQLFSKL 571
           F+++IPRSLAGKILRKDLVSQFKQQQ+ SKL
Sbjct: 541 FMSSIPRSLAGKILRKDLVSQFKQQQVLSKL 564

[Close]

BLAST of MC09g1396 vs. ExPASy TrEMBL
Match: A0A6J1H2E6 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458939 PE=4 SV=1)

HSP 1 Score: 937 bits (2423), Expect = 0.0
Identity = 486/567 (85.71%), Postives = 524/567 (92.42%), Query Frame = 0


Query: 1   MSGEESYTAPAAGEYPRQNAPPPPLSKGGYDVNTAVYHSLVKFDEARKISSRPDLDTATF 60
           MS EES TAPAA E+   N+P      GGYDVNTAVYHSLV+FDEA KIS+ PDLDTATF
Sbjct: 1   MSREESCTAPAAVEFRPINSP------GGYDVNTAVYHSLVQFDEAPKISTSPDLDTATF 60

Query: 61  VLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSIL 120
           VLSQFP AESRVALIDSVTSSRVTYG+LSAS RSLA GL+HALGVRKGDVVFVLSANS+L
Sbjct: 61  VLSQFPTAESRVALIDSVTSSRVTYGKLSASTRSLAYGLYHALGVRKGDVVFVLSANSVL 120

Query: 121 YPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTT 180
           YPVICLAVLS+GAVITTANPVNTASEI KQV DS AKLA+SAPEE+HKL+PTG+PTILTT
Sbjct: 121 YPVICLAVLSIGAVITTANPVNTASEIGKQVSDSCAKLAISAPEELHKLSPTGVPTILTT 180

Query: 181 RPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240
           RPS GD VLSVEELIESC +S E +P T++ QSDTAAILYSSGTTGTSKGVVLTHSNLIS
Sbjct: 181 RPSYGD-VLSVEELIESCIESFEQMPKTKIAQSDTAAILYSSGTTGTSKGVVLTHSNLIS 240

Query: 241 VIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAI 300
           VIELLT YVDASSS++DVFLCF+PMFHIYGLVFFGLGLFCRG TI+LMQRFNFQ+MIDAI
Sbjct: 241 VIELLTWYVDASSSQDDVFLCFVPMFHIYGLVFFGLGLFCRGTTIVLMQRFNFQSMIDAI 300

Query: 301 EKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPG 360
           E YKVNNIPAVPPVILGLVKS GGSDLSSLRRIGSGAAPLGKD+E+AFREKFPWVELRPG
Sbjct: 301 ETYKVNNIPAVPPVILGLVKSSGGSDLSSLRRIGSGAAPLGKDVEEAFREKFPWVELRPG 360

Query: 361 YGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPT 420
           YGLTESTGAATC+ITD DA+ HPGSCGMLMPRF AKIVDIETGEGLPPMKEGE+WLKSPT
Sbjct: 361 YGLTESTGAATCMITDKDARAHPGSCGMLMPRFCAKIVDIETGEGLPPMKEGEVWLKSPT 420

Query: 421 IMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480
           IMK YL N+EATEATMD+EGW+KTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET
Sbjct: 421 IMKEYLRNKEATEATMDKEGWMKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELET 480

Query: 481 ILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVK 540
           IL+ HTEILDAAVIP EDEAAGQIPVACVVKA + +LTE+QVIQFVASQVA YKK+RGV+
Sbjct: 481 ILLSHTEILDAAVIPAEDEAAGQIPVACVVKAPSCKLTEEQVIQFVASQVAAYKKIRGVR 540

Query: 541 FINAIPRSLAGKILRKDLVSQFKQQQL 567
           F+++IPRSLAGKILRKDLVSQ KQQ L
Sbjct: 541 FMSSIPRSLAGKILRKDLVSQLKQQVL 560

[Close]

BLAST of MC09g1396 vs. ExPASy TrEMBL
Match: A0A1S3BFZ3 (4-coumarate--CoA ligase-like 5 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489425 PE=4 SV=1)

HSP 1 Score: 936 bits (2419), Expect = 0.0
Identity = 491/573 (85.69%), Postives = 525/573 (91.62%), Query Frame = 0


Query: 1   MSGEESY-TAPAAGEYPRQNAPPPPLSKGGYDVNTAVYHSLVKFDEARKISSRPDLDTAT 60
           MS EES  T PAA E+  +N+P      GGY+VNTAVYHSL+ FDEA  IS+R DLDTAT
Sbjct: 1   MSDEESCSTGPAAMEFQPRNSP------GGYNVNTAVYHSLIHFDEAPVISTRSDLDTAT 60

Query: 61  FVLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSI 120
           +VLSQFP AESRVALIDSVTS RVTYGQL  SIRSLACGL+HALGVRKGDVVFVLSAN +
Sbjct: 61  YVLSQFPTAESRVALIDSVTSLRVTYGQLGVSIRSLACGLYHALGVRKGDVVFVLSANCV 120

Query: 121 LYPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILT 180
           LYPVICLAVLS+GAVITTANP+NTASEI KQVRDSGAKLAVSAPEE+ KL PTGIPTILT
Sbjct: 121 LYPVICLAVLSIGAVITTANPINTASEIGKQVRDSGAKLAVSAPEELQKLRPTGIPTILT 180

Query: 181 TRPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLI 240
           TR SS  D LSVEELIESC++SSE LP TEVTQSDTAAILYSSGTTGTSKGVVLTHSNLI
Sbjct: 181 TR-SSFSDALSVEELIESCSESSEPLPKTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLI 240

Query: 241 SVIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDA 300
           SVIELLT  VD++SS++DVFLCFIPMFHIYGLVFFGLGLFCRGIT +LMQRFNFQ+MIDA
Sbjct: 241 SVIELLTWCVDSTSSQHDVFLCFIPMFHIYGLVFFGLGLFCRGITTVLMQRFNFQSMIDA 300

Query: 301 IEKYKVNNIPAVPPVILGLVKSGGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRP 360
           IEKYK+NNIPAVPPVILGLVKSGGGSD SSLRR+GSGAAPLGKD+E+AFREKFPWVELRP
Sbjct: 301 IEKYKINNIPAVPPVILGLVKSGGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRP 360

Query: 361 GYGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSP 420
           GYGLTESTGAAT +ITD DAK HPGSCGMLMP F  KIVD+ETGEGLPPMK+GELWLKSP
Sbjct: 361 GYGLTESTGAATWMITDKDAKAHPGSCGMLMPSFYGKIVDVETGEGLPPMKKGELWLKSP 420

Query: 421 TIMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELE 480
           TIMK YLGN+EATEAT+DEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELE
Sbjct: 421 TIMKEYLGNREATEATLDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELE 480

Query: 481 TILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGV 540
           TIL+ HTEILDAAVIP+EDEAAGQIPVACVVKA +  LTE QVIQFVASQVAPYKKVRGV
Sbjct: 481 TILLSHTEILDAAVIPIEDEAAGQIPVACVVKAPSCVLTEQQVIQFVASQVAPYKKVRGV 540

Query: 541 KFINAIPRSLAGKILRKDLVSQFKQQQ-LFSKL 571
           +FI+AIPRSLAGKILRKDLVSQFKQQQ LFSKL
Sbjct: 541 RFISAIPRSLAGKILRKDLVSQFKQQQQLFSKL 566

[Close]

BLAST of MC09g1396 vs. ExPASy TrEMBL
Match: A0A0A0KRI4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622450 PE=4 SV=1)

HSP 1 Score: 905 bits (2339), Expect = 0.0
Identity = 471/550 (85.64%), Postives = 506/550 (92.00%), Query Frame = 0


Query: 23  PPLSKGGYDVNTAVYHSLVKFDEARKISSRPDLDTATFVLSQFPKAESRVALIDSVTSSR 82
           P  S GGY+VNTAVYHSL+  DEA  IS+R DLDTAT+VLSQFP AESRVALIDSVTS R
Sbjct: 11  PQSSPGGYNVNTAVYHSLLHLDEAPVISTRSDLDTATYVLSQFPTAESRVALIDSVTSLR 70

Query: 83  VTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSILYPVICLAVLSVGAVITTANPVN 142
           VTYGQLS SIRSLACGL+HALGVRKGDVVFVLSAN +LYPVICLAVLS+GAVITTANP+N
Sbjct: 71  VTYGQLSVSIRSLACGLYHALGVRKGDVVFVLSANCVLYPVICLAVLSIGAVITTANPMN 130

Query: 143 TASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTTRPSSGDDVLSVEELIESCADSS 202
           T SEI KQVRDSGAKLAVSAPEE+ KL+PTGIPTILTTR S GD  LSVEELIESC++SS
Sbjct: 131 TESEIGKQVRDSGAKLAVSAPEELQKLSPTGIPTILTTRSSYGD-ALSVEELIESCSESS 190

Query: 203 -ESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTLYVDASSSENDVFLC 262
            E LP  EVT SDTAAILYSSGTTGTSKGVVLTHSNLISVIE+LT  VD++SS++DVFLC
Sbjct: 191 SEPLPKAEVTPSDTAAILYSSGTTGTSKGVVLTHSNLISVIEILTWCVDSTSSQHDVFLC 250

Query: 263 FIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAIEKYKVNNIPAVPPVILGLVKS 322
           FIPMFHIYGLVFFGLGLF RGIT +LM RFNFQ+MIDAIEKYK+NNIPAVPPVILGLVKS
Sbjct: 251 FIPMFHIYGLVFFGLGLFRRGITTVLMPRFNFQSMIDAIEKYKINNIPAVPPVILGLVKS 310

Query: 323 GGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPGYGLTESTGAATCIITDTDAKT 382
            GGSD SSLRR+GSGAAPLGKD+E+AFREKFPWVELRPGYGLTESTGAAT +ITD DAK 
Sbjct: 311 DGGSDFSSLRRVGSGAAPLGKDVEEAFREKFPWVELRPGYGLTESTGAATWMITDKDAKA 370

Query: 383 HPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNQEATEATMDEEGW 442
           HPGSCGMLMP F  KIVD+ETGEGLPPMK+GELWLKSPTIMK YLGN+EATEAT+DEEGW
Sbjct: 371 HPGSCGMLMPSFYGKIVDVETGEGLPPMKKGELWLKSPTIMKEYLGNREATEATIDEEGW 430

Query: 443 LKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILVCHTEILDAAVIPMEDEAA 502
           LKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETIL+ HTEILDAAVIP+EDEAA
Sbjct: 431 LKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILLSHTEILDAAVIPIEDEAA 490

Query: 503 GQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVKFINAIPRSLAGKILRKDLVSQ 562
           GQIPVACVVKA + +L+E QVIQFVASQVAPYKKVRGV+FI+AIPRSLAGKILRKDLVSQ
Sbjct: 491 GQIPVACVVKAPSCELSEQQVIQFVASQVAPYKKVRGVRFISAIPRSLAGKILRKDLVSQ 550

Query: 563 FKQQQLFSKL 571
           FKQ QL SKL
Sbjct: 551 FKQLQLLSKL 559

[Close]

BLAST of MC09g1396 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 441.8 bits (1135), Expect = 8.3e-124
Identity = 239/515 (46.41%), Postives = 343/515 (66.60%), Query Frame = 0


Query: 53  PDLDTATFVLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVF 112
           P LD  TF+ SQ  +   R+A ID+ T   +T+ +L  ++ S+A  L   +G+RKG VV 
Sbjct: 30  PSLDVTTFISSQAHR--GRIAFIDASTGQNLTFTELWRAVESVADCLSE-IGIRKGHVVL 89

Query: 113 VLSANSILYPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPT 172
           +LS NSIL+PV+CL+V+S+GA+ITT NP+NT++EIAKQ++DS   LA +  + + K++  
Sbjct: 90  LLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAA 149

Query: 173 G--IPTIL--TTRPSSGDDVLSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTS 232
              +P +L    R  S  DV  + E+++   + S +     V Q DTA +LYSSGTTG S
Sbjct: 150 AKKLPIVLMDEERVDSVGDVRRLVEMMKK--EPSGNRVKERVDQDDTATLLYSSGTTGMS 209

Query: 233 KGVVLTHSNLISVIELLTLYVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILM 292
           KGV+ +H NLI++++ +     +   E   F+C +PMFHIYGL  F  GL   G TII++
Sbjct: 210 KGVISSHRNLIAMVQTIVNRFGSDDGEQR-FICTVPMFHIYGLAAFATGLLAYGSTIIVL 269

Query: 293 QRFNFQAMIDAIEKYKVNNIPAVPPVILGLVKSG----GGSDLSSLRRIGSGAAPLGKDI 352
            +F    M+ AI KY+  ++P VPP+++ +V          DLSS+  +  G APL K++
Sbjct: 270 SKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEV 329

Query: 353 EDAFREKFPWVELRPGYGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGE 412
            + F EK+P V++  GYGLTESTG      T  +++ + G+ G L      +IVD  TG+
Sbjct: 330 TEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRY-GTAGKLSASMEGRIVDPVTGQ 389

Query: 413 GLPPMKEGELWLKSPTIMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKE 472
            L P + GELWLK P+IMKGY  N+EAT +T+D EGWL+TGDL YIDEDGF+++VDR+KE
Sbjct: 390 ILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKE 449

Query: 473 LIKHNGYQVAPAELETILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQ 532
           LIK+ GYQVAPAELE +L+ H EI DAAVIP  D+  GQ P+A VV+ + S L+E  +++
Sbjct: 450 LIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIME 509

Query: 533 FVASQVAPYKKVRGVKFINAIPRSLAGKILRKDLV 560
           FVA QVAPYK++R V F+++IP++ +GKILRKDL+
Sbjct: 510 FVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLI 537

[Close]

BLAST of MC09g1396 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 439.5 bits (1129), Expect = 4.1e-123
Identity = 241/550 (43.82%), Postives = 344/550 (62.55%), Query Frame = 0


Query: 25  LSKGGYDVNTAVYHSLVKFDEARKISSRPDLDTATFVLSQFPKAESRVALIDSVTSSRVT 84
           + K GY     +Y SL        +   P+    +F+        S++A+ DS T   +T
Sbjct: 1   MEKSGYG-RDGIYRSL---RPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLT 60

Query: 85  YGQLSASIRSLACGLHHALGVRKGDVVFVLSANSILYPVICLAVLSVGAVITTANPVNTA 144
           + QL +++  LA G H  LG+RK DVV + + NS  +P+  LAV ++G V TTANP+ T 
Sbjct: 61  FSQLKSAVARLAHGFHR-LGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTV 120

Query: 145 SEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTIL-------TTRPSSGDDVLSVEELIES 204
           +E++KQ++DS  K+ +S  +   K+    +P +L          P S   +LS + ++E 
Sbjct: 121 NEVSKQIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMEL 180

Query: 205 CADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTLYVDASSSEND 264
               SE  P  E+ QSDTAA+LYSSGTTGTSKGV LTH N I+   ++T+  D     + 
Sbjct: 181 SEPVSE-YPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHG 240

Query: 265 VFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAIEKYKVNNIPAVPPVILG 324
           VFLCF+PMFH++GL         RG  ++ M RF  + ++  IEK++V ++  VPPV L 
Sbjct: 241 VFLCFLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLA 300

Query: 325 LVKSG--GGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPGYGLTESTGAATCIIT 384
           L K       DLSSL+ IGSGAAPLGKD+ +      P V L  GYG+TE+ G  + +  
Sbjct: 301 LSKQSIVKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVS-VED 360

Query: 385 DTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNQEATEAT 444
               K + GS GML P   A+IV +ETG+  PP ++GE+W++ P +MKGYL N +AT+ T
Sbjct: 361 PRLGKRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKET 420

Query: 445 MDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILVCHTEILDAAVIP 504
           +D++ W+ TGDLGY +EDG LY+VDRIKELIK+ G+QVAPAELE +LV H +ILDA VIP
Sbjct: 421 IDKKSWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIP 480

Query: 505 MEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVKFINAIPRSLAGKILR 564
             DE AG++P+A VV++  S +TE  + +F+A QVAPYK++R V FI+ +P+S AGKILR
Sbjct: 481 FPDEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILR 540

Query: 565 KDLVSQFKQQ 566
           ++LV Q + +
Sbjct: 541 RELVQQVRSK 543

[Close]

BLAST of MC09g1396 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 417.9 bits (1073), Expect = 1.3e-116
Identity = 235/556 (42.27%), Postives = 347/556 (62.41%), Query Frame = 0


Query: 15  YPRQNAPPPPLSKGGYDVNTAVYHSLVKFDEARKISSRPDL--DTATFVLSQFPKAESRV 74
           YP ++    P  + G+  + + ++S     + + +S  P+L  D  TF+ SQ      + 
Sbjct: 3   YPERSLIVDP--RSGFCKSNSTFYS-----KRQPLSLPPNLSRDVTTFISSQ--PHRGKT 62

Query: 75  ALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVFVLSANSILYPVICLAVLSVG 134
           A ID+ T   +T+  L  ++  +A  L+H +G+R+GDVV +LS NSI  PV+CL+V+S+G
Sbjct: 63  AFIDAATGQCLTFSDLWRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLG 122

Query: 135 AVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPTGIPTILTTRPSSGDDVLSVE 194
           AV TTAN +NT+ EI+KQ+ DS   L  +  +   KL P  I  +LT       ++ S  
Sbjct: 123 AVFTTANTLNTSGEISKQIADSNPTLVFTTRQLAPKL-PVAISVVLTD-DEVYQELTSAI 182

Query: 195 ELIESCADSSESLPNTE-----VTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTL 254
            ++   ++  +  P+ +     V Q DTA +LYSSGTTG SKGV+ +H NL + +     
Sbjct: 183 RVVGILSEMVKKEPSGQRVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHV---AR 242

Query: 255 YVDASSSENDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAIEKYKVNN 314
           ++  +   +D+F+C +PMFH YGL+ F +G    G T+++++RF    M+DA+EK++   
Sbjct: 243 FISDNLKRDDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATA 302

Query: 315 IPAVPPVILGLVKSG----GGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPGYGL 374
           +   PPV++ ++          DLSSL+ +  G APL K++ + F EK+P V++  GY L
Sbjct: 303 LALAPPVLVAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYAL 362

Query: 375 TESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPTIMK 434
           TES G      +  +++ + G+ G L     A+IVD  TG  +   + GELWLK P+I K
Sbjct: 363 TESNGGGAFTNSAEESRRY-GTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISK 422

Query: 435 GYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILV 494
           GY  NQEAT  T++ EGWLKTGDL YIDEDGFL++VDR+KELIK+ GYQV PAELE +L+
Sbjct: 423 GYFKNQEATNETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLI 482

Query: 495 CHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVKFIN 554
            H +ILDAAVIP  D+ AGQ P+A VV+   S L+E QVI F++ QVAPYKK+R V FIN
Sbjct: 483 THPDILDAAVIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFIN 542

Query: 555 AIPRSLAGKILRKDLV 560
           +IP++ +GK LRKDL+
Sbjct: 543 SIPKTASGKTLRKDLI 543

[Close]

BLAST of MC09g1396 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 416.0 bits (1068), Expect = 4.9e-116
Identity = 239/547 (43.69%), Postives = 350/547 (63.99%), Query Frame = 0


Query: 27  KGGYDVNTAVYHSLVKFDEARKISSRPDLDTATFVLSQFPKAESRVALIDSVTSSRVTYG 86
           K G+  +T++++S     E   +     LD  +F+ SQ      +   +D+VT  R+++ 
Sbjct: 24  KSGFCESTSIFYSK---REPMALPPNQFLDVTSFIASQ--PHRGKTVFVDAVTGRRLSFP 83

Query: 87  QLSASIRSLACGLHHALGVRKGDVVFVLSANSILYPVICLAVLSVGAVITTANPVNTASE 146
           +L   +  +A G  +ALGVRKG+VV +LS NSIL+P++ L+V+S+GA+ITTANP+NT+ E
Sbjct: 84  ELWLGVERVA-GCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDE 143

Query: 147 IAKQVRDSGAKLAVSAPEEIHKL---NPTGIPTIL-----TTRPSSGDDVLSV---EELI 206
           I+KQ+ DS   LA +  + + KL   +   +P +L         S GD V  V   E +I
Sbjct: 144 ISKQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMI 203

Query: 207 ESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGVVLTHSNLISVIELLTLYVDASSSE 266
           E+  + SES     V Q DTAA+LYSSGTTGTSKGV+L+H NLI++++       A    
Sbjct: 204 ET--EPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYR----ARFGL 263

Query: 267 NDVFLCFIPMFHIYGLVFFGLGLFCRGITIILMQRFNFQAMIDAIEKYKVNNIPAVPPVI 326
               +C IPM HI+G   F  GL   G TI+++ +F+   ++ A+E ++ + +  VPP++
Sbjct: 264 EQRTICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIV 323

Query: 327 LGLVKS----GGGSDLSSLRRIGSGAAPLGKDIEDAFREKFPWVELRPGYGLTESTGAAT 386
           + +V          DLSSL  + +G APL +++ + F E +P V++  GYGLTEST  A 
Sbjct: 324 VAMVNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAA 383

Query: 387 CIITDTDAKTHPGSCGMLMPRFSAKIVDIETGEGLPPMKEGELWLKSPTIMKGYLGNQEA 446
            +    + K + G+ G+L P    KIVD +TG  L   + GELW++SPT+MKGY  N+EA
Sbjct: 384 SMFNKEETKRY-GASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEA 443

Query: 447 TEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKELIKHNGYQVAPAELETILVCHTEILDA 506
           T +T+D EGWLKTGDL YID DGF+++VDR+KELIK NGYQVAPAELE +L+ H EI DA
Sbjct: 444 TASTIDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADA 503

Query: 507 AVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQFVASQVAPYKKVRGVKFINAIPRSLAG 559
           AVIP+ D  AGQ P+A +V+   S L+E +++ FVA QV+PYKK+R V F+ +IP++ +G
Sbjct: 504 AVIPIPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSG 557

[Close]

BLAST of MC09g1396 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 409.5 bits (1051), Expect = 4.6e-114
Identity = 221/514 (43.00%), Postives = 333/514 (64.79%), Query Frame = 0


Query: 53  PDLDTATFVLSQFPKAESRVALIDSVTSSRVTYGQLSASIRSLACGLHHALGVRKGDVVF 112
           P+LD  + + S   K     ALIDS+T   +++ +L   ++S+A G++H LGVR+GDVV 
Sbjct: 45  PNLDAVSALFSH--KHHGDTALIDSLTGFSISHTELQIMVQSMAAGIYHVLGVRQGDVVS 104

Query: 113 VLSANSILYPVICLAVLSVGAVITTANPVNTASEIAKQVRDSGAKLAVSAPEEIHKLNPT 172
           ++  NS+ +P+I L+++S+GA++TT NP ++  EI KQV +    LA ++ E + KL+  
Sbjct: 105 LVLPNSVYFPMIFLSLISLGAIVTTMNPSSSLGEIKKQVSECSVGLAFTSTENVEKLSSL 164

Query: 173 GIPTILTTRPSSGDDV-LSVEELIESCADSSESLPNTEVTQSDTAAILYSSGTTGTSKGV 232
           G+  I  +     D + +   +      +S   +P   + Q D AAI+YSSGTTG SKGV
Sbjct: 165 GVSVISVSESYDFDSIRIENPKFYSIMKESFGFVPKPLIKQDDVAAIMYSSGTTGASKGV 224

Query: 233 VLTHSNLISVIELLTLYVDASSSE----NDVFLCFIPMFHIYGLVFFGLGLFCRGITIIL 292
           +LTH NLI+ +EL   + +AS  E    ++V+L  +P+ HIYGL  F +GL   G TI++
Sbjct: 225 LLTHRNLIASMELFVRF-EASQYEYPGSSNVYLAALPLCHIYGLSLFVMGLLSLGSTIVV 284

Query: 293 MQRFNFQAMIDAIEKYKVNNIPAVPPVILGLVKSGG---GSDLSSLRRIGSGAAPLGKDI 352
           M+RF+   +++ IE++K+ + P VPP+++ L K      G    SL+++ SGAAPL +  
Sbjct: 285 MKRFDASDVVNVIERFKITHFPVVPPMLMALTKKAKGVCGEVFKSLKQVSSGAAPLSRKF 344

Query: 353 EDAFREKFPWVELRPGYGLTESTGAATCIITDTDAKTHPGSCGMLMPRFSAKIVDIETGE 412
            + F +  P V+L  GYG+TEST   T    +++  +   S G+L P   AK+VD  +G 
Sbjct: 345 IEDFLQTLPHVDLIQGYGMTESTAVGTRGF-NSEKLSRYSSVGLLAPNMQAKVVDWSSGS 404

Query: 413 GLPPMKEGELWLKSPTIMKGYLGNQEATEATMDEEGWLKTGDLGYIDEDGFLYIVDRIKE 472
            LPP   GELW++ P +MKGYL N +AT+ ++ E+ WL+TGD+ Y DEDG+L+IVDRIKE
Sbjct: 405 FLPPGNRGELWIQGPGVMKGYLNNPKATQMSIVEDSWLRTGDIAYFDEDGYLFIVDRIKE 464

Query: 473 LIKHNGYQVAPAELETILVCHTEILDAAVIPMEDEAAGQIPVACVVKASTSQLTEDQVIQ 532
           +IK+ G+Q+APA+LE +LV H  I+DAAV    +E  G+IPVA VV+   + L+E+ VI 
Sbjct: 465 IIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGEIPVAFVVRRQETTLSEEDVIS 524

Query: 533 FVASQVAPYKKVRGVKFINAIPRSLAGKILRKDL 559
           +VASQVAPY+KVR V  +N+IP+S  GKILRK+L
Sbjct: 525 YVASQVAPYRKVRKVVMVNSIPKSPTGKILRKEL 554

The following BLAST results are available for this feature:

BLAST of MC09g1396 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
M4IQR7	3.3e-125	45.60	Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1	[more]
Q84P21	1.2e-122	46.41	4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=...	[more]
Q9M0X9	5.8e-122	43.82	4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=...	[more]
Q10S72	5.1e-118	44.96	4-coumarate--CoA ligase-like 4 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL4...	[more]
M4IRL6	5.1e-118	45.87	Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1	[more]

BLAST of MC09g1396 vs. NCBI nr


Match Name	Identity	Description
XP_022138968.1	99.82	4-coumarate--CoA ligase-like 5 [Momordica charantia]	[more]
XP_038891768.1	85.64	4-coumarate--CoA ligase-like 5 [Benincasa hispida]	[more]
XP_022994494.1	85.46	4-coumarate--CoA ligase-like 5 [Cucurbita maxima]	[more]
XP_022957594.1	85.71	4-coumarate--CoA ligase 1-like [Cucurbita moschata]	[more]
KAG7031989.1	85.54	4-coumarate--CoA ligase-like 5 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MC09g1396 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1CEK1	99.82	4-coumarate--CoA ligase-like 5 OS=Momordica charantia OX=3673 GN=LOC111010017 PE...	[more]
A0A6J1K303	85.46	4-coumarate--CoA ligase-like 5 OS=Cucurbita maxima OX=3661 GN=LOC111490173 PE=4 ...	[more]
A0A6J1H2E6	85.71	4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458939 PE=...	[more]
A0A1S3BFZ3	85.69	4-coumarate--CoA ligase-like 5 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10348942...	[more]
A0A0A0KRI4	85.64	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622450 PE=4 SV=1	[more]

BLAST of MC09g1396 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G20510.1	8.3e-124	46.41	OPC-8:0 CoA ligase1	[more]
AT4G05160.1	4.1e-123	43.82	AMP-dependent synthetase and ligase family protein	[more]
AT1G20500.1	1.3e-116	42.27	AMP-dependent synthetase and ligase family protein	[more]
AT1G20480.1	4.9e-116	43.69	AMP-dependent synthetase and ligase family protein	[more]
AT4G19010.1	4.6e-114	43.00	AMP-dependent synthetase and ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 42..463 e-value: 4.0E-128 score: 429.8
None	No IPR available	GENE3D	3.30.300.30		coord: 464..568 e-value: 1.8E-27 score: 98.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..27
None	No IPR available	PANTHER	PTHR24096:SF376	AMP-DEPENDENT SYNTHETASE AND LIGASE FAMILY PROTEIN-RELATED	coord: 22..564
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 22..564
None	No IPR available	CDD	cd05904	4CL	coord: 49..558 e-value: 0.0 score: 742.515
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 62..564
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 69..467 e-value: 5.8E-103 score: 344.7
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 477..552 e-value: 2.6E-12 score: 47.5
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 218..229

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g1396.1	MC09g1396.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009698	phenylpropanoid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016207	4-coumarate-CoA ligase activity