MC09g1293 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC09g1293
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Cysteine proteinase inhibitor
Location	MC09: 18996639 .. 19000205 (-)
RNA-Seq Expression	MC09g1293
Synteny	MC09g1293

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GTATTTGATATATTATTGATATAAATGAAAATTTTTTGCTAGATTTATAAATTTTAAAAATAGGTAATATATTTTAGAAATACATCGAAGATTTTTTGTTATCAACGATAATTTTTCAAATTTTAAATTACAGAAATTTAATTGTTGCATACTAAATTATAAAATAGTTCACAAATTTAATTTGTGAACGGATTTAAATTAAAAAATGGGCGACTCAACCAATAACCGGTGGGATTTAAATTTGAACTACAAGAAAACGGAAGATTTTTGTACTCGAAAAAAAAAGAAAAGAAAAAAAGATTTAAATGCTTAGCTACGAAGCAACGCTTCGTGGCGCAGTTGCTTGTTTGGCACGAATCCAGATTCTTACATTCGTGGCGCGAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGGTTTCAATTAGGATAATCTACCGTTACAGCGAAGGAATTTCACAAAATCGAAAAAAAATGAGGCGATCGTGATCTCTGTTATGAGAAAGGACTCTTCGATTTTGTTTTCTTCATCTACGCTGTTGCTGCTGTTGCTCTATTGGATCGTACTCGGAGGAAACGCATCAGCTCACTTTTGTAGGCAAGAAGACGATCCACTGCTGATGGCGTCCACACTTGGAGGCGTGCACGATTCTCAGGGAGCTTCCAATTCCGCCGGTGTCGAAGAACTCGCTCGTTTCGCCGTCGACGAACACAACAAGAAAGAGGTAAAGATGTTCTCTCTCTGTAGCTTCTATTTGCTTCGTTCTCGGTTCGAGTGGTTCTTTTGTCGGTTCGGTGTGTTTCTTTCTCGAATTTCATCGATTCTCGTTGATGCGTCTGTGCCGTCAGTGCCTGATCTTTCTCTAGTTCTAGTTGTGCTGTGTTATGGACATCTGTGTGTTAGGCGTCATTTACTTCGCAATGGCGGATCTTGTGTTAACGTGCTCTGCCTTCTGATGCGATCGGTTTCTTTCGGAGTTGATAATTAGTATCACGACGTGGTCTACCAGAGCTGCGCGGTCTTCCTCTTGTTTCGTTTGTTTGGTAGAATTGATTATGGTTATATTCTTATTTTTTTTAGATTCTGGATTGCGTTTTTAGGTTGGAGCTGGGGATATGTGTAATGTCTCTTTAGCTTGAAAAAGTTAATAGATCTTTATGGCTATAATTAATTAGAATCCACATTCAATTTTTCAAAAATCTGCTACACCAGTAGACTTGCCGTAAGATGATGACTGTAACGTGATTGCAAGGCCTTATGCCGACCTTCTCTATAATATCTTTTTTAGGCTGATGGAAACTAGGTCAGCGAATGGTCCCTCCGTTTTGGTTATAATTCGTGGAATTCTGGTGACTTCGAGCTTTCAGTTTACCTTCTAGTCACTTTTAGTTACTTAAACACATCTGGATGCCTTCACCAGAAATTGATGTTTCTTCATTTCTCACATGGAATCACGTCCCAAGTATTTGTACATTAATTTTATTTCAGTTTAAAGAGTCCTCTTTCTATCGTGTCCCAAGTATTTGTATATTGATTTTATCAGTATTCAGGAGAAATTTTATTTCAGTTTAATGGGTTCTTTTTCCACAGCTAACTAAGAAGTTTTTCTTTTGACAAGCATCTGTAATGGATTCAAAGCATTGGAAATTGGGTGTACAGAAGCATAGGAGTGTAGTTGTTTGCAATTTAAAATTCCTCTTGTTCCTTTTTTCTGTTTTCATGCAACTGTAATAACACCCGTATACTGGTCACTTGTGTTATACATGTCTATAATGTAAGTATCATCCTACTCCTTTGCAGAATGCACTTATTGAGTTTGTGAGAGTGGCGAAGGCGCAAGAGCAGGTAGTAGCTGGCACACTGCACCATCTCACTCTTGAGGCTGTTGACGCTGGTAAAAAGAAGCTGTATGAAGCTAAGGTTTGGGTGAAGCCATGGATGAACTTTAAAGAACTGCAAGAGTTCAAGCATGCAGGCGATGTCCCTGCATTTACTCCTTCTGATCTTGGTGCAAAGAAAGGTGGCTGACATTCTTTAACTCATTAATACTGTTTCTTTAAGTTTAACTTCAAATATACAACCAATGTCTGGTATGTATAGAAGTCGTGCACTTGATCTTTCTTTGCATGAGCAGTAATTTTTGGTTCAAGTCATTCATTACTTCTGCTTGTTGGCTGATCAAATATAAGGTTATGTTTTTTATCTTTTCTAATTTACTAAAGGTAAAAATTCTTGCACGTGATCCCAGATCCTTGCATATGAATAAACTTGCATTTGGATTGTAGTGCCTATTTAGTGAGCTCCTTTCCTATATCTCATCTATGTAATTGTGGTGAATCGGTGATGGTAGCTCTATTGCAAAATCATAATCCATCGTTAGCATTAGTATTTCTCACAAGCTTATTAGCGCTGTATGTTTGCAACTTTGCGTTATATTCATATAGACGATAAAAGCTGTAATTTATTTGTCATTCTTGTAACTGGTTTGACAGAAAATCAGTGTTATCTTTTCTATATTACTGACCATGGTTATAAATCAGGCGATCATCCGCAGGGATGGAGAGAAGTGCCCCCGCATGATCCTCAAGTTCAGGATGCAGCACATCATGCTGTTCAGACCATCCAGCAGAGATCTAATTCTCTATTCCCATATGAATTGCTGGAGATCATACATGCAAAGGCAGAGGTCAGTGCATTATGGTCCCTTTCATTTTCTGGATTACTTGCTTCTCTCTTGAAATTTCACATGATTGTGGAGTTATGTTTGTTCTTACTTTTCGCGTAACTGAAACTTATCATTTTTATTTTAGATTTTAGACTTGTGTATGATTGACCATAAAGTTACATTATCTGGTTAATTCGGTCCCCTGAAGGCATCAAGTTTGAATTTTCGTAGGACTCACTCAATTATTTACATTGCTTTCACCCATTTCCTTTAGAGCTTTTATGATTACATCATTTTGATTAGCTCAACTTTAAACGGACTGTCGATTCTATCAAACTAAATACTAGTGATACATTTTACCCGAGAAAAAGGTAGGTGTTTTGTTTGTTAACGTATTGTAAAATAGCCAAATTTCGAACTCCAAGATGTTGTTTATTCATTCCCCATGTGCACTTTATGACAAATTTCTGAACATTCTAAATATAATCGCTAATGGATTCTCACAACATTATGAAAACAGGTGGTTGAAGATTCTGCGAAGTTCGATTTGCACCTAAAGGTCATGAGAGGAAGTAAAGAAGAGAAGTTCAAGGTGAAGGTCCATAAGAACAATGAAGGTAACTTCCTTCTGAATCAGATGGTGGCAGATCAGTCTTAGGGGAGTTTGGAATGCGCAATAATAAAGATGAACAATATACAAGTTTTATGTAATGATAAAACATTTGGATTAGTTAAGCTGTAAACTATGCAAACGTGAACCTTGTCTTTTAAACTTAAATTGCTCCCACCCCTCGAGTCTGGGGATGCCTGTGCCATGAAAAATAGTGGAGCTACCCTGTTTGTTAAGTAATGGAACTCTGGTTTACTTTTTTACTCGGTTTGAAATTAGGGTTGACTAGTTT

mRNA sequence

GTATTTGATATATTATTGATATAAATGAAAATTTTTTGCTAGATTTATAAATTTTAAAAATAGGTAATATATTTTAGAAATACATCGAAGATTTTTTGTTATCAACGATAATTTTTCAAATTTTAAATTACAGAAATTTAATTGTTGCATACTAAATTATAAAATAGTTCACAAATTTAATTTGTGAACGGATTTAAATTAAAAAATGGGCGACTCAACCAATAACCGGTGGGATTTAAATTTGAACTACAAGAAAACGGAAGATTTTTGTACTCGAAAAAAAAAGAAAAGAAAAAAAGATTTAAATGCTTAGCTACGAAGCAACGCTTCGTGGCGCAGTTGCTTGTTTGGCACGAATCCAGATTCTTACATTCGTGGCGCGAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGGTTTCAATTAGGATAATCTACCGTTACAGCGAAGGAATTTCACAAAATCGAAAAAAAATGAGGCGATCGTGATCTCTGTTATGAGAAAGGACTCTTCGATTTTGTTTTCTTCATCTACGCTGTTGCTGCTGTTGCTCTATTGGATCGTACTCGGAGGAAACGCATCAGCTCACTTTTGTAGGCAAGAAGACGATCCACTGCTGATGGCGTCCACACTTGGAGGCGTGCACGATTCTCAGGGAGCTTCCAATTCCGCCGGTGTCGAAGAACTCGCTCGTTTCGCCGTCGACGAACACAACAAGAAAGAGAATGCACTTATTGAGTTTGTGAGAGTGGCGAAGGCGCAAGAGCAGGTAGTAGCTGGCACACTGCACCATCTCACTCTTGAGGCTGTTGACGCTGGTAAAAAGAAGCTGTATGAAGCTAAGGTTTGGGTGAAGCCATGGATGAACTTTAAAGAACTGCAAGAGTTCAAGCATGCAGGCGATGTCCCTGCATTTACTCCTTCTGATCTTGGTGCAAAGAAAGGCGATCATCCGCAGGGATGGAGAGAAGTGCCCCCGCATGATCCTCAAGTTCAGGATGCAGCACATCATGCTGTTCAGACCATCCAGCAGAGATCTAATTCTCTATTCCCATATGAATTGCTGGAGATCATACATGCAAAGGCAGAGGTGGTTGAAGATTCTGCGAAGTTCGATTTGCACCTAAAGGTCATGAGAGGAAGTAAAGAAGAGAAGTTCAAGGTGAAGGTCCATAAGAACAATGAAGGTAACTTCCTTCTGAATCAGATGGTGGCAGATCAGTCTTAGGGGAGTTTGGAATGCGCAATAATAAAGATGAACAATATACAAGTTTTATGTAATGATAAAACATTTGGATTAGTTAAGCTGTAAACTATGCAAACGTGAACCTTGTCTTTTAAACTTAAATTGCTCCCACCCCTCGAGTCTGGGGATGCCTGTGCCATGAAAAATAGTGGAGCTACCCTGTTTGTTAAGTAATGGAACTCTGGTTTACTTTTTTACTCGGTTTGAAATTAGGGTTGACTAGTTT

Coding sequence (CDS)

ATGAGAAAGGACTCTTCGATTTTGTTTTCTTCATCTACGCTGTTGCTGCTGTTGCTCTATTGGATCGTACTCGGAGGAAACGCATCAGCTCACTTTTGTAGGCAAGAAGACGATCCACTGCTGATGGCGTCCACACTTGGAGGCGTGCACGATTCTCAGGGAGCTTCCAATTCCGCCGGTGTCGAAGAACTCGCTCGTTTCGCCGTCGACGAACACAACAAGAAAGAGAATGCACTTATTGAGTTTGTGAGAGTGGCGAAGGCGCAAGAGCAGGTAGTAGCTGGCACACTGCACCATCTCACTCTTGAGGCTGTTGACGCTGGTAAAAAGAAGCTGTATGAAGCTAAGGTTTGGGTGAAGCCATGGATGAACTTTAAAGAACTGCAAGAGTTCAAGCATGCAGGCGATGTCCCTGCATTTACTCCTTCTGATCTTGGTGCAAAGAAAGGCGATCATCCGCAGGGATGGAGAGAAGTGCCCCCGCATGATCCTCAAGTTCAGGATGCAGCACATCATGCTGTTCAGACCATCCAGCAGAGATCTAATTCTCTATTCCCATATGAATTGCTGGAGATCATACATGCAAAGGCAGAGGTGGTTGAAGATTCTGCGAAGTTCGATTTGCACCTAAAGGTCATGAGAGGAAGTAAAGAAGAGAAGTTCAAGGTGAAGGTCCATAAGAACAATGAAGGTAACTTCCTTCTGAATCAGATGGTGGCAGATCAGTCTTAG

Protein sequence

MRKDSSILFSSSTLLLLLLYWIVLGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSAGVEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWVKPWMNFKELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQRSNSLFPYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNFLLNQMVADQS

Homology

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BLAST of MC09g1293 vs. ExPASy Swiss-Prot
Match: Q0JNR2 (Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0270100 PE=2 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 3.7e-78
Identity = 147/229 (64.19%), Postives = 179/229 (78.17%), Query Frame = 0


Query: 10  SSSTLLLLLLYWIVLGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSAGVEELARFAV 69
           SS+   L L   + +   A+A F        +    LGG HD+  A+NS   + LARFAV
Sbjct: 12  SSAAAPLPLFLLLAVAAAAAALFLVGSASLAMAGHVLGGAHDAPSAANSVETDALARFAV 71

Query: 70  DEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWVKPWMNFKELQ 129
           DEHNK+ENAL+EFVRV +A+EQVVAGTLHHLTLEA++AG+KK+YEAKVWVKPW++FKELQ
Sbjct: 72  DEHNKRENALLEFVRVVEAKEQVVAGTLHHLTLEALEAGRKKVYEAKVWVKPWLDFKELQ 131

Query: 130 EFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQRSNSLFPYEL 189
           EF++ GD   FT +DLGAKKG H  GWR+VP HDP V+DAA HAV++IQQRSNSLFPYEL
Sbjct: 132 EFRNTGDATTFTNADLGAKKGGHEPGWRDVPVHDPVVKDAADHAVKSIQQRSNSLFPYEL 191

Query: 190 LEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNFLLNQM 239
           LEI+ AKAEVVED AKFD+ +K+ RG+KEEKFK +VHKN EG F+LNQM
Sbjct: 192 LEIVRAKAEVVEDFAKFDILMKLKRGNKEEKFKAEVHKNLEGAFVLNQM 240

[Close]

BLAST of MC09g1293 vs. ExPASy Swiss-Prot
Match: Q8H0X6 (Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=2)

HSP 1 Score: 273.5 bits (698), Expect = 2.3e-72
Identity = 145/230 (63.04%), Postives = 172/230 (74.78%), Query Frame = 0


Query: 7   ILFSSSTLLLLLLYWIVLGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSAGVEELAR 66
           I+F S +L +  L    LG      FC +E       + +GGV D     NS  VE LAR
Sbjct: 10  IVFFSLSLFISSLIASDLG------FCNEE------MALVGGVGDVPANQNSGEVESLAR 69

Query: 67  FAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWVKPWMNFK 126
           FAVDEHNKKENAL+EF RV KA+EQVVAGTLHHLTLE ++AG+KKLYEAKVWVKPW+NFK
Sbjct: 70  FAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFK 129

Query: 127 ELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQRSNSLFP 186
           ELQEFK A D PA T SDLG K+G+H  GWREVP  DP+V+  A  AV+TIQQRSNSLFP
Sbjct: 130 ELQEFKPASDAPAITSSDLGCKQGEHESGWREVPGDDPEVKHVAEQAVKTIQQRSNSLFP 189

Query: 187 YELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNFLLN 237
           YELLE++HAKAEV  ++AK+++ LK+ RG KEEKFKV+VHKN+EG   LN
Sbjct: 190 YELLEVVHAKAEVTGEAAKYNMLLKLKRGEKEEKFKVEVHKNHEGALHLN 227

[Close]

BLAST of MC09g1293 vs. ExPASy Swiss-Prot
Match: Q8LC76 (Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=2)

HSP 1 Score: 209.9 bits (533), Expect = 3.2e-53
Identity = 111/228 (48.68%), Postives = 162/228 (71.05%), Query Frame = 0


Query: 10  SSSTLLLLLLYWIVLGGNASAHFCRQED---DPLLMASTLGGVHDSQGASNSA-GVEELA 69
           +S  ++L+ +  ++L G      C +E    +  ++   LGG  DS+   N    ++++A
Sbjct: 6   ASMCMMLICVSLVLLSGFGQFVICSEEKGTYNDNVVKMKLGGFSDSKNDWNGGKEIDDIA 65

Query: 70  RFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWVKPWMNF 129
            FAV EHN++ENA++E  RV KA EQVVAG L+ LTLE ++AG+KK+YEAKVWVKPWMNF
Sbjct: 66  LFAVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEVIEAGEKKIYEAKVWVKPWMNF 125

Query: 130 KELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQRSNSLF 189
           K+LQEFK+   +P+FT SDLG K   +   WR V  ++P+VQ+AA HA++++QQ+SNSLF
Sbjct: 126 KQLQEFKNI--IPSFTISDLGFKPDGNGFDWRSVSTNNPEVQEAAKHAMKSLQQKSNSLF 185

Query: 190 PYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNF 234
           PY+L++II A+A+VVE+  KF+L LK+ RG+K EKF V+V K+  G +
Sbjct: 186 PYKLIDIILARAKVVEERVKFELLLKLERGNKLEKFMVEVMKDQTGKY 231

[Close]

BLAST of MC09g1293 vs. ExPASy Swiss-Prot
Match: Q06445 (Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 7.6e-31
Identity = 64/96 (66.67%), Postives = 77/96 (80.21%), Query Frame = 0


Query: 44  STLGGVHDSQGASNSAGVEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLE 103
           + LGG  D  G  NS  ++ LARFAV+EHNKK+NAL+EF RV  AQ+QVV+GTL+ +TLE
Sbjct: 2   AALGGNRDVAGNQNSLEIDSLARFAVEEHNKKQNALLEFGRVVSAQQQVVSGTLYTITLE 61

Query: 104 AVDAGKKKLYEAKVWVKPWMNFKELQEFKHAGDVPA 140
           A D G+KK+YEAKVW KPW+NFKELQEFKH GD PA
Sbjct: 62  AKDGGQKKVYEAKVWEKPWLNFKELQEFKHVGDAPA 97

[Close]

BLAST of MC09g1293 vs. ExPASy Swiss-Prot
Match: Q41906 (Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=CYS3 PE=2 SV=2)

HSP 1 Score: 134.8 bits (338), Expect = 1.3e-30
Identity = 64/118 (54.24%), Postives = 83/118 (70.34%), Query Frame = 0


Query: 20  YWIV---LGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSAGVEELARFAVDEHNKKE 79
           +WIV   L G      CR E+        LGGVHD +G  NS  +E LARFA+ EHNK++
Sbjct: 6   FWIVTLLLCGTIQLAICRSEEKSTEKTMMLGGVHDLRGNQNSGEIESLARFAIQEHNKQQ 65

Query: 80  NALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWVKPWMNFKELQEFKHA 135
           N ++EF ++ KA+EQVVAGT++HLTLEA +  + K +EAKVWVKPWMNFK+LQEFK +
Sbjct: 66  NKILEFKKIVKAREQVVAGTMYHLTLEAKEGDQTKNFEAKVWVKPWMNFKQLQEFKES 123

[Close]

BLAST of MC09g1293 vs. NCBI nr
Match: XP_022139142.1 (cysteine proteinase inhibitor 12 [Momordica charantia])

HSP 1 Score: 486 bits (1250), Expect = 4.99e-173
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0


Query: 1   MRKDSSILFSSSTLLLLLLYWIVLGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSAG 60
           MRKDSSILFSSSTLLLLLLYWIVLGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSAG
Sbjct: 1   MRKDSSILFSSSTLLLLLLYWIVLGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSAG 60

Query: 61  VEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWVK 120
           VEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWVK
Sbjct: 61  VEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWVK 120

Query: 121 PWMNFKELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQR 180
           PWMNFKELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQR
Sbjct: 121 PWMNFKELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQR 180

Query: 181 SNSLFPYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNFLLNQMVA 240
           SNSLFPYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNFLLNQMVA
Sbjct: 181 SNSLFPYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNFLLNQMVA 240

Query: 241 DQS 243
           DQS
Sbjct: 241 DQS 243

[Close]

BLAST of MC09g1293 vs. NCBI nr
Match: XP_022957234.1 (cysteine proteinase inhibitor 12-like [Cucurbita moschata])

HSP 1 Score: 421 bits (1081), Expect = 2.98e-147
Identity = 208/244 (85.25%), Postives = 225/244 (92.21%), Query Frame = 0


Query: 1   MRKDSSILFSSST-LLLLLLYWIVLGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSA 60
           M+ DSSI FSSST LLLLLL WIVL GNASAHFC Q+DDPLLMASTLGGVHDSQGASNSA
Sbjct: 1   MKNDSSIWFSSSTFLLLLLLSWIVLAGNASAHFCAQQDDPLLMASTLGGVHDSQGASNSA 60

Query: 61  GVEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWV 120
             +ELARFAVDEHNKKEN+L+EFVRV KA+EQVVAGTLHHLT+E  DAGKKKLYEAK+WV
Sbjct: 61  DFDELARFAVDEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVADAGKKKLYEAKIWV 120

Query: 121 KPWMNFKELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQ 180
           K WMNFKELQEFKHAGD P+F+PSDLGAKKGDHPQGWREVPPHDP VQDAA HA++TIQQ
Sbjct: 121 KSWMNFKELQEFKHAGDAPSFSPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQ 180

Query: 181 RSNSLFPYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNFLLNQMV 240
           RSNSL PYELLEI+HAKAEV+ED+AKFDL LK+ RGSKEEKFKV+VHKNNEGNFLLNQMV
Sbjct: 181 RSNSLLPYELLEIVHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMV 240

Query: 241 ADQS 243
            DQS
Sbjct: 241 PDQS 244

[Close]

BLAST of MC09g1293 vs. NCBI nr
Match: XP_023548422.1 (cysteine proteinase inhibitor 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 419 bits (1077), Expect = 1.21e-146
Identity = 207/244 (84.84%), Postives = 226/244 (92.62%), Query Frame = 0


Query: 1   MRKDSSILFSSSTLLLLLLY-WIVLGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSA 60
           M+ DSSI FSSSTLLLLLL  WIVL GNASAHFC Q+D+PLLMASTLGGVHDSQGASNSA
Sbjct: 1   MKNDSSIWFSSSTLLLLLLLSWIVLAGNASAHFCAQQDNPLLMASTLGGVHDSQGASNSA 60

Query: 61  GVEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWV 120
            V+ELARFAVDEHNKKEN+L+EFVRV KA+EQVVAGTLHHLT+E  DAGKKKLYEAK+WV
Sbjct: 61  DVDELARFAVDEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVADAGKKKLYEAKIWV 120

Query: 121 KPWMNFKELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQ 180
           K WMNFKELQEFKHAGD P+F+PSDLGAKKGDHPQGWREVPPHDP VQDAA HA++TIQQ
Sbjct: 121 KSWMNFKELQEFKHAGDAPSFSPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQ 180

Query: 181 RSNSLFPYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNFLLNQMV 240
           RSNSL PYELLEI+HAKAEV+ED+AKFDL LK+ RGSKEEKFKV+VHKN+EGNFLLNQMV
Sbjct: 181 RSNSLLPYELLEIVHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNSEGNFLLNQMV 240

Query: 241 ADQS 243
            DQS
Sbjct: 241 PDQS 244

[Close]

BLAST of MC09g1293 vs. NCBI nr
Match: XP_022986762.1 (cysteine proteinase inhibitor 12-like [Cucurbita maxima])

HSP 1 Score: 417 bits (1073), Expect = 4.93e-146
Identity = 208/244 (85.25%), Postives = 224/244 (91.80%), Query Frame = 0


Query: 1   MRKDSSILFSSST-LLLLLLYWIVLGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSA 60
           M+ DSSI FSSST LLLLLL WIVL GNASAHFC Q+DDPLLMASTLGGVHDSQGASNSA
Sbjct: 1   MKNDSSIWFSSSTFLLLLLLSWIVLAGNASAHFCAQQDDPLLMASTLGGVHDSQGASNSA 60

Query: 61  GVEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWV 120
            V+ELARFAVDEHNKKENAL+EFVRV KA+EQVVAGTLHHLT+E  DAGKKKLYEAK+WV
Sbjct: 61  DVDELARFAVDEHNKKENALLEFVRVVKAKEQVVAGTLHHLTVEVADAGKKKLYEAKIWV 120

Query: 121 KPWMNFKELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQ 180
           K WMNFKELQEFKHAGD P+F+PSDLGAKKGDHPQGWREVP HDP VQDAA HA++ IQQ
Sbjct: 121 KSWMNFKELQEFKHAGDAPSFSPSDLGAKKGDHPQGWREVPLHDPHVQDAAQHALRIIQQ 180

Query: 181 RSNSLFPYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNFLLNQMV 240
           RSNSL PYELLEI+HAKAEV+ED+AKFDL LK+ RGSKEEKFKV+VHKNNEGNFLLNQMV
Sbjct: 181 RSNSLLPYELLEIVHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMV 240

Query: 241 ADQS 243
            DQS
Sbjct: 241 PDQS 244

[Close]

BLAST of MC09g1293 vs. NCBI nr
Match: KAG7031902.1 (Cysteine proteinase inhibitor 12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 417 bits (1071), Expect = 9.95e-146
Identity = 205/244 (84.02%), Postives = 224/244 (91.80%), Query Frame = 0


Query: 1   MRKDSSILFSSST-LLLLLLYWIVLGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSA 60
           M+ DSSI FSSST LLLLLL WIVL GNA+AHFC Q+DDPLLMASTLGGVHD QGASNSA
Sbjct: 1   MKNDSSIWFSSSTFLLLLLLSWIVLAGNAAAHFCAQQDDPLLMASTLGGVHDCQGASNSA 60

Query: 61  GVEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWV 120
            ++ELARFAVDEHNKKEN+ +EFVRV KA+EQVVAGTLHHLT+E  DAGKKKLYEAK+WV
Sbjct: 61  DLDELARFAVDEHNKKENSFLEFVRVVKAKEQVVAGTLHHLTVEVADAGKKKLYEAKIWV 120

Query: 121 KPWMNFKELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQ 180
           K WMNFKELQEFKHAGD P+F+PSDLGAKKGDHPQGWREVPPHDP VQDAA HA++TIQQ
Sbjct: 121 KSWMNFKELQEFKHAGDAPSFSPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQ 180

Query: 181 RSNSLFPYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNFLLNQMV 240
           RSNSL PYELLEI+HAKAEV+ED+AKFDL LK+ RGSKEEKFKV+VHKNNEGNFLLNQMV
Sbjct: 181 RSNSLLPYELLEIVHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMV 240

Query: 241 ADQS 243
            DQS
Sbjct: 241 PDQS 244

[Close]

BLAST of MC09g1293 vs. ExPASy TrEMBL
Match: A0A6J1CD67 (Cysteine proteinase inhibitor OS=Momordica charantia OX=3673 GN=LOC111010119 PE=3 SV=1)

HSP 1 Score: 486 bits (1250), Expect = 2.41e-173
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0


Query: 1   MRKDSSILFSSSTLLLLLLYWIVLGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSAG 60
           MRKDSSILFSSSTLLLLLLYWIVLGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSAG
Sbjct: 1   MRKDSSILFSSSTLLLLLLYWIVLGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSAG 60

Query: 61  VEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWVK 120
           VEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWVK
Sbjct: 61  VEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWVK 120

Query: 121 PWMNFKELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQR 180
           PWMNFKELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQR
Sbjct: 121 PWMNFKELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQR 180

Query: 181 SNSLFPYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNFLLNQMVA 240
           SNSLFPYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNFLLNQMVA
Sbjct: 181 SNSLFPYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNFLLNQMVA 240

Query: 241 DQS 243
           DQS
Sbjct: 241 DQS 243

[Close]

BLAST of MC09g1293 vs. ExPASy TrEMBL
Match: A0A6J1H1C9 (Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111458681 PE=3 SV=1)

HSP 1 Score: 421 bits (1081), Expect = 1.44e-147
Identity = 208/244 (85.25%), Postives = 225/244 (92.21%), Query Frame = 0


Query: 1   MRKDSSILFSSST-LLLLLLYWIVLGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSA 60
           M+ DSSI FSSST LLLLLL WIVL GNASAHFC Q+DDPLLMASTLGGVHDSQGASNSA
Sbjct: 1   MKNDSSIWFSSSTFLLLLLLSWIVLAGNASAHFCAQQDDPLLMASTLGGVHDSQGASNSA 60

Query: 61  GVEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWV 120
             +ELARFAVDEHNKKEN+L+EFVRV KA+EQVVAGTLHHLT+E  DAGKKKLYEAK+WV
Sbjct: 61  DFDELARFAVDEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVADAGKKKLYEAKIWV 120

Query: 121 KPWMNFKELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQ 180
           K WMNFKELQEFKHAGD P+F+PSDLGAKKGDHPQGWREVPPHDP VQDAA HA++TIQQ
Sbjct: 121 KSWMNFKELQEFKHAGDAPSFSPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHALRTIQQ 180

Query: 181 RSNSLFPYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNFLLNQMV 240
           RSNSL PYELLEI+HAKAEV+ED+AKFDL LK+ RGSKEEKFKV+VHKNNEGNFLLNQMV
Sbjct: 181 RSNSLLPYELLEIVHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMV 240

Query: 241 ADQS 243
            DQS
Sbjct: 241 PDQS 244

[Close]

BLAST of MC09g1293 vs. ExPASy TrEMBL
Match: A0A6J1J8G5 (Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111484419 PE=3 SV=1)

HSP 1 Score: 417 bits (1073), Expect = 2.39e-146
Identity = 208/244 (85.25%), Postives = 224/244 (91.80%), Query Frame = 0


Query: 1   MRKDSSILFSSST-LLLLLLYWIVLGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSA 60
           M+ DSSI FSSST LLLLLL WIVL GNASAHFC Q+DDPLLMASTLGGVHDSQGASNSA
Sbjct: 1   MKNDSSIWFSSSTFLLLLLLSWIVLAGNASAHFCAQQDDPLLMASTLGGVHDSQGASNSA 60

Query: 61  GVEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWV 120
            V+ELARFAVDEHNKKENAL+EFVRV KA+EQVVAGTLHHLT+E  DAGKKKLYEAK+WV
Sbjct: 61  DVDELARFAVDEHNKKENALLEFVRVVKAKEQVVAGTLHHLTVEVADAGKKKLYEAKIWV 120

Query: 121 KPWMNFKELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQ 180
           K WMNFKELQEFKHAGD P+F+PSDLGAKKGDHPQGWREVP HDP VQDAA HA++ IQQ
Sbjct: 121 KSWMNFKELQEFKHAGDAPSFSPSDLGAKKGDHPQGWREVPLHDPHVQDAAQHALRIIQQ 180

Query: 181 RSNSLFPYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNFLLNQMV 240
           RSNSL PYELLEI+HAKAEV+ED+AKFDL LK+ RGSKEEKFKV+VHKNNEGNFLLNQMV
Sbjct: 181 RSNSLLPYELLEIVHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNFLLNQMV 240

Query: 241 ADQS 243
            DQS
Sbjct: 241 PDQS 244

[Close]

BLAST of MC09g1293 vs. ExPASy TrEMBL
Match: A0A5D3CW86 (Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002960 PE=3 SV=1)

HSP 1 Score: 399 bits (1024), Expect = 8.63e-139
Identity = 204/250 (81.60%), Postives = 222/250 (88.80%), Query Frame = 0


Query: 1   MRKDSSILFS--SSTLLLLLLY----WI-VLGGNASAHFCRQEDDPLLMASTLGGVHDSQ 60
           M   S+I FS  SSTL LLLL     WI VL GNASAHFC ++DDPLLMASTLGGVHDSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSAGVEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLY 120
           GASNS  V+ELARFAV+EHNKKEN+L+EFVRV KA+EQVVAGTLHHLT+E V+AGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKPWMNFKELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHA 180
           EAKVWVK WMNFKELQEFKHAGDVP+ +PSDLGAKKGDHPQGWREVPPHDP VQDAA HA
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180

Query: 181 VQTIQQRSNSLFPYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNF 240
           ++TIQQRSNSL PYELLEIIHAKAEV+ED+AKFDL LK+ RGSKEEKFKV+VHKNNEGNF
Sbjct: 181 LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF 240

Query: 241 LLNQMVADQS 243
           LLNQMV D S
Sbjct: 241 LLNQMVQDHS 250

[Close]

BLAST of MC09g1293 vs. ExPASy TrEMBL
Match: A0A1S3BEK2 (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103488820 PE=3 SV=1)

HSP 1 Score: 399 bits (1024), Expect = 8.63e-139
Identity = 204/250 (81.60%), Postives = 222/250 (88.80%), Query Frame = 0


Query: 1   MRKDSSILFS--SSTLLLLLLY----WI-VLGGNASAHFCRQEDDPLLMASTLGGVHDSQ 60
           M   S+I FS  SSTL LLLL     WI VL GNASAHFC ++DDPLLMASTLGGVHDSQ
Sbjct: 1   MNNHSTISFSPLSSTLSLLLLLLFFSWIIVLAGNASAHFCAEQDDPLLMASTLGGVHDSQ 60

Query: 61  GASNSAGVEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLY 120
           GASNS  V+ELARFAV+EHNKKEN+L+EFVRV KA+EQVVAGTLHHLT+E V+AGKKKLY
Sbjct: 61  GASNSTDVDELARFAVEEHNKKENSLLEFVRVVKAKEQVVAGTLHHLTVEVVEAGKKKLY 120

Query: 121 EAKVWVKPWMNFKELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHA 180
           EAKVWVK WMNFKELQEFKHAGDVP+ +PSDLGAKKGDHPQGWREVPPHDP VQDAA HA
Sbjct: 121 EAKVWVKSWMNFKELQEFKHAGDVPSISPSDLGAKKGDHPQGWREVPPHDPHVQDAAQHA 180

Query: 181 VQTIQQRSNSLFPYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNF 240
           ++TIQQRSNSL PYELLEIIHAKAEV+ED+AKFDL LK+ RGSKEEKFKV+VHKNNEGNF
Sbjct: 181 LRTIQQRSNSLVPYELLEIIHAKAEVIEDAAKFDLLLKLKRGSKEEKFKVEVHKNNEGNF 240

Query: 241 LLNQMVADQS 243
           LLNQMV D S
Sbjct: 241 LLNQMVQDHS 250

[Close]

BLAST of MC09g1293 vs. TAIR 10
Match: AT3G12490.2 (cystatin B )

HSP 1 Score: 273.5 bits (698), Expect = 1.7e-73
Identity = 145/230 (63.04%), Postives = 172/230 (74.78%), Query Frame = 0


Query: 7   ILFSSSTLLLLLLYWIVLGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSAGVEELAR 66
           I+F S +L +  L    LG      FC +E       + +GGV D     NS  VE LAR
Sbjct: 10  IVFFSLSLFISSLIASDLG------FCNEE------MALVGGVGDVPANQNSGEVESLAR 69

Query: 67  FAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWVKPWMNFK 126
           FAVDEHNKKENAL+EF RV KA+EQVVAGTLHHLTLE ++AG+KKLYEAKVWVKPW+NFK
Sbjct: 70  FAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFK 129

Query: 127 ELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQRSNSLFP 186
           ELQEFK A D PA T SDLG K+G+H  GWREVP  DP+V+  A  AV+TIQQRSNSLFP
Sbjct: 130 ELQEFKPASDAPAITSSDLGCKQGEHESGWREVPGDDPEVKHVAEQAVKTIQQRSNSLFP 189

Query: 187 YELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNFLLN 237
           YELLE++HAKAEV  ++AK+++ LK+ RG KEEKFKV+VHKN+EG   LN
Sbjct: 190 YELLEVVHAKAEVTGEAAKYNMLLKLKRGEKEEKFKVEVHKNHEGALHLN 227

[Close]

BLAST of MC09g1293 vs. TAIR 10
Match: AT3G12490.1 (cystatin B )

HSP 1 Score: 273.5 bits (698), Expect = 1.7e-73
Identity = 135/191 (70.68%), Postives = 157/191 (82.20%), Query Frame = 0


Query: 46  LGGVHDSQGASNSAGVEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAV 105
           +GGV D     NS  VE LARFAVDEHNKKENAL+EF RV KA+EQVVAGTLHHLTLE +
Sbjct: 4   VGGVGDVPANQNSGEVESLARFAVDEHNKKENALLEFARVVKAKEQVVAGTLHHLTLEIL 63

Query: 106 DAGKKKLYEAKVWVKPWMNFKELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQ 165
           +AG+KKLYEAKVWVKPW+NFKELQEFK A D PA T SDLG K+G+H  GWREVP  DP+
Sbjct: 64  EAGQKKLYEAKVWVKPWLNFKELQEFKPASDAPAITSSDLGCKQGEHESGWREVPGDDPE 123

Query: 166 VQDAAHHAVQTIQQRSNSLFPYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKV 225
           V+  A  AV+TIQQRSNSLFPYELLE++HAKAEV  ++AK+++ LK+ RG KEEKFKV+V
Sbjct: 124 VKHVAEQAVKTIQQRSNSLFPYELLEVVHAKAEVTGEAAKYNMLLKLKRGEKEEKFKVEV 183

Query: 226 HKNNEGNFLLN 237
           HKN+EG   LN
Sbjct: 184 HKNHEGALHLN 194

[Close]

BLAST of MC09g1293 vs. TAIR 10
Match: AT5G05110.1 (Cystatin/monellin family protein )

HSP 1 Score: 209.9 bits (533), Expect = 2.3e-54
Identity = 111/228 (48.68%), Postives = 162/228 (71.05%), Query Frame = 0


Query: 10  SSSTLLLLLLYWIVLGGNASAHFCRQED---DPLLMASTLGGVHDSQGASNSA-GVEELA 69
           +S  ++L+ +  ++L G      C +E    +  ++   LGG  DS+   N    ++++A
Sbjct: 6   ASMCMMLICVSLVLLSGFGQFVICSEEKGTYNDNVVKMKLGGFSDSKNDWNGGKEIDDIA 65

Query: 70  RFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWVKPWMNF 129
            FAV EHN++ENA++E  RV KA EQVVAG L+ LTLE ++AG+KK+YEAKVWVKPWMNF
Sbjct: 66  LFAVQEHNRRENAVLELARVLKATEQVVAGKLYRLTLEVIEAGEKKIYEAKVWVKPWMNF 125

Query: 130 KELQEFKHAGDVPAFTPSDLGAKKGDHPQGWREVPPHDPQVQDAAHHAVQTIQQRSNSLF 189
           K+LQEFK+   +P+FT SDLG K   +   WR V  ++P+VQ+AA HA++++QQ+SNSLF
Sbjct: 126 KQLQEFKNI--IPSFTISDLGFKPDGNGFDWRSVSTNNPEVQEAAKHAMKSLQQKSNSLF 185

Query: 190 PYELLEIIHAKAEVVEDSAKFDLHLKVMRGSKEEKFKVKVHKNNEGNF 234
           PY+L++II A+A+VVE+  KF+L LK+ RG+K EKF V+V K+  G +
Sbjct: 186 PYKLIDIILARAKVVEERVKFELLLKLERGNKLEKFMVEVMKDQTGKY 231

[Close]

BLAST of MC09g1293 vs. TAIR 10
Match: AT2G40880.1 (cystatin A )

HSP 1 Score: 134.8 bits (338), Expect = 9.2e-32
Identity = 64/118 (54.24%), Postives = 83/118 (70.34%), Query Frame = 0


Query: 20  YWIV---LGGNASAHFCRQEDDPLLMASTLGGVHDSQGASNSAGVEELARFAVDEHNKKE 79
           +WIV   L G      CR E+        LGGVHD +G  NS  +E LARFA+ EHNK++
Sbjct: 6   FWIVTLLLCGTIQLAICRSEEKSTEKTMMLGGVHDLRGNQNSGEIESLARFAIQEHNKQQ 65

Query: 80  NALIEFVRVAKAQEQVVAGTLHHLTLEAVDAGKKKLYEAKVWVKPWMNFKELQEFKHA 135
           N ++EF ++ KA+EQVVAGT++HLTLEA +  + K +EAKVWVKPWMNFK+LQEFK +
Sbjct: 66  NKILEFKKIVKAREQVVAGTMYHLTLEAKEGDQTKNFEAKVWVKPWMNFKQLQEFKES 123

[Close]

BLAST of MC09g1293 vs. TAIR 10
Match: AT5G12140.1 (cystatin-1 )

HSP 1 Score: 101.7 bits (252), Expect = 8.7e-22
Identity = 50/92 (54.35%), Postives = 61/92 (66.30%), Query Frame = 0


Query: 46  LGGVHDSQGASNSAGVEELARFAVDEHNKKENALIEFVRVAKAQEQVVAGTLHHLTLEAV 105
           +GGV D    +N   VE LARFAVDEHNK EN  +E+ R+  A+ QVVAGT+HHLT+E  
Sbjct: 10  VGGVRDIDANANDLQVESLARFAVDEHNKNENLTLEYKRLLGAKTQVVAGTMHHLTVEVA 69

Query: 106 DAGKKKLYEAKVWVKPWMNFKELQEFKHAGDV 138
           D    K+YEAKV  K W N K+L+ F H  DV
Sbjct: 70  DGETNKVYEAKVLEKAWENLKQLESFNHLHDV 101

The following BLAST results are available for this feature:

BLAST of MC09g1293 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0JNR2	3.7e-78	64.19	Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]
Q8H0X6	2.3e-72	63.04	Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=...	[more]
Q8LC76	3.2e-53	48.68	Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=...	[more]
Q06445	7.6e-31	66.67	Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1	[more]
Q41906	1.3e-30	54.24	Cysteine proteinase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=CYS3 PE=2 SV=...	[more]

BLAST of MC09g1293 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139142.1	4.99e-173	100.00	cysteine proteinase inhibitor 12 [Momordica charantia]	[more]
XP_022957234.1	2.98e-147	85.25	cysteine proteinase inhibitor 12-like [Cucurbita moschata]	[more]
XP_023548422.1	1.21e-146	84.84	cysteine proteinase inhibitor 12-like [Cucurbita pepo subsp. pepo]	[more]
XP_022986762.1	4.93e-146	85.25	cysteine proteinase inhibitor 12-like [Cucurbita maxima]	[more]
KAG7031902.1	9.95e-146	84.02	Cysteine proteinase inhibitor 12 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MC09g1293 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CD67	2.41e-173	100.00	Cysteine proteinase inhibitor OS=Momordica charantia OX=3673 GN=LOC111010119 PE=...	[more]
A0A6J1H1C9	1.44e-147	85.25	Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111458681 PE=3...	[more]
A0A6J1J8G5	2.39e-146	85.25	Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111484419 PE=3 S...	[more]
A0A5D3CW86	8.63e-139	81.60	Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3BEK2	8.63e-139	81.60	Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103488820 PE=3 SV=1	[more]

BLAST of MC09g1293 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12490.2	1.7e-73	63.04	cystatin B	[more]
AT3G12490.1	1.7e-73	70.68	cystatin B	[more]
AT5G05110.1	2.3e-54	48.68	Cystatin/monellin family protein	[more]
AT2G40880.1	9.2e-32	54.24	cystatin A	[more]
AT5G12140.1	8.7e-22	54.35	cystatin-1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	SMART	SM00043	CY_4	coord: 151..242 e-value: 0.16 score: 9.5 coord: 44..134 e-value: 4.5E-31 score: 119.2
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 59..133 e-value: 1.5E-20 score: 73.3
IPR000010	Cystatin domain	PFAM	PF00031	Cystatin	coord: 157..227 e-value: 2.1E-5 score: 24.7
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 47..133 e-value: 4.28782E-23 score: 88.3
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 155..200 e-value: 1.4343E-5 score: 40.9204
None	No IPR available	GENE3D	3.10.450.10		coord: 42..140 e-value: 1.6E-42 score: 146.1
None	No IPR available	GENE3D	3.10.450.10		coord: 148..238 e-value: 6.3E-13 score: 50.6
None	No IPR available	PANTHER	PTHR11413:SF103	CYSTEINE PROTEINASE INHIBITOR 6	coord: 45..239
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 48..136
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 148..234
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 45..239
IPR018073	Proteinase inhibitor I25, cystatin, conserved site	PROSITE	PS00287	CYSTATIN	coord: 90..103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC09g1293.1	MC09g1293.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity